Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr04

Orthogroup ID

OG0003973

Physical Location & Seq

Genomic Coordinates Reverse (-)

29761499 .. 29765685

Gene Structure 4187 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD04G1214400.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2427 bp

ATGAAAACGATCGTGTCCGGTTCGGATGAACCGAAGTTGAAGCAGGGGAATCTGGCCCTGCAGTGGAAACACTTCTTGGACCACTTTGACTCGCTCAAGACCCGGTTTGAGGAGCTCCAGGACCGTTTTTACTCGGAGCGGAACTCGCTCCAGACACGGCAGCGGGAGGTTGAGGAGCGCGAGAAGGGGTTGGAGGCCAAAGTTTTGAACTTTAACTCGGAAATGGCGTCTAAAGCTCAGGAATTGTATGAAATCGAGAGATTGGCGAAAGAGAAAAGAAGAGAGGTTGGTAGTAAGGAAAAATGGTTGTTGGAAAACGAGGAATCGGTTAGGGAGAAGGAGAGGGAACGTGATTTGATAAAAAATGATATCGAAGAGCGAACGGAGAAATTGAATTCGGTTGTGAAAAGTTTGGATGAGAAGTCGAGAGAGCTGAAGATGAAGAAGGAGGTCGCTGAGTTGAACGGAAGGCGATTAAATGCGATTAAGGGGTCTATTGACGATGTCATGAAAGATTTTGTTTCGAAACAAGAGCAGGTTAAAGCGGCGCATAAATCTCTCGAAGAATGTCATAAGGAGATTCAATCGAAAAGGACGATACTTGGTGCCGTTGAAAAATCGATAAGGGAATGTTACCGTACACTCGAATCCAAAGAGAAAAGCATCAGGGCAGTGGAGTTGAAACAACAACAATTTGAGTTGATGGTTCAAGAGTGCCAGAAGCATTTGGATTCACAAGAAAAGTTGTTGCAACAACGTTGCCATGGACTCGAAATGAAAGAGAGGCAACTCGAAGAGCAGGTCAGAGTGTTTGAATCTAAACAACGAGAATTTGATTTGATGGTTAAAGAACGCCAAAAATGTTTGGATTTGCAAGAAAAGTTGTTACAAGAAGGTTGCCACGGACTTGAAAAGAAAGAAAGGCGACTTGAAGAAAAGGTCAGAGAGTTTGAATCGAATAAAAAAGAATTTGATTCGATGATTCAAGAACGCCAACAACATTTGGATTCTGAAGAGAAGTTGTTACAAGAAGGTTCCCATGGACTTAAAATGAAAGAGAGGCAGCTTGAAGAACAGGTCAGAAAGCTCGAATCAGAACAAAAACAATTTGAATCGCTGCAAAAATCCAAGGAAGACATCCAAAACCTGAAATCCAAGGAGAAGACTAATTGTTCCAGGGATGGCAAATGCTTGCAGGTTCTCATGAATGAGAATTGGACGAGAACTGATCTTGTATGTGGCGAAATCGCAGCTTGTCTTCAGGCGTCATCTGACCCAGCAAAGCTCGTTTTGGATGCAATGCAAGGGTTTTATCCTTCAAATCAGACAGTGGACACCACTGAGTTTGCCTTTGATTTGACAGTTATTAGGAGGAGTTGTATTTTTTTGTTACAGCAGTTGAAGAGATTCTCACCACAAATCAACCCTCAGGTGAGAAAAGAAGCAAGGGCTTTGGCAGCTGAATGGAAGGGTAAGATGACTGCGGCGACTGGGAATGGATTGGAGATTTTGGGTTTTTTGGAGCTTGTTGCTGTCTATGAAATCACAACTGTTTATGATTCAAAGGAGCTTCAGAGTCTTCTTGGTACAGTGGCTGAGCATGAGCAAGGAACTGAGCTATGCCAGGCCCTTGGTATCACAACTAGCAACAGCGTTTCTTTCCCCATCAAAATTGAGGAACAAGAATCTTCACAACTTATAAATGTCGCAGCTTTATCTCCTCTGGATCTTCAACCGAGTGCAACCACAGATACAAGGAATTTGCAGGGCTCTACAGATGAGCATTTAAGTGAGAAAGAGTCCGTACAGAACGAAATGCTTCTTGATCTACAAATGTCAATGGACCCAGCAGAACTTGTTTTAAAGTTGATTAAAGAATCTTTGGCTCAATGCTGGAGAAAAAGAGATGTTGGCTGTGAATCAGCTGTCATGGAGAATAGCATTTCCCTGTTGAATGATCTAACGGAAGTCTCCATGAAAATTGGGCCAGATGTTAAAAAAAATGCAATAGAGCTAGCAGTCCAGTGGAAAGCAAAAATGAGAGTTGATTCTGAAAATTCAATTGAGATTTTGGGGTTTTTGCACTTTATAGCTACATATGGATTGGTTTCTACCTTTAACAGAGATGAGATTGTAAAGCTTCTTGGGACGATTTGTCTGCACAAAAAGGCTTTGGAATCATGCCAGGCAATTGGTTTTGCAGATATGATCCCTGATTTTGTTCAAGATCTCATTGAAAGGAAGCAACTGATTGAAGCTGTTAGATTGATTTGCGGCTTCAACTTATCTGCCAGGTTCCCTCCGGTACCACTCTTAAAAGAATACTTAAAAGAATATGTGGAAGGTGCAAGGCAGTCTTACTGCACAAAATGGCTGGTAAAAAGATCACTTGATGAAAGGGTACATACATCTTTGGACTTCCAATAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Frigida

Protein Analysis

809

Amino Acids

93.41

Weight (kDa)

5.72

Isoelectric Point (pI)

55.53

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Frigida	PF07899	393 - 547	2.9e-31	Frigida-like protein
Frigida	PF07899	593 - 787	1e-43	Frigida-like protein

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0003973)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G27220 AT5G27220 AT5G27220 AT5G27220
fragaria_vesca	FvH4_6g04390
malus_domestica	MD04G1214300.v1.1 MD04G1214400.v1.1 MD04G1214500.v1.1 MD12G1228900.v1.1 MD12G1229100.v1.1
prunus_persica	Prupe.6G334100_v2.0.a1 Prupe.6G334200_v2.0.a1 Prupe.6G334200_v2.0.a1 Prupe.6G334200_v2.0.a1 Prupe.6G334400_v2.0.a1 Prupe.6G334500_v2.0.a1
pyrus_communis	pycom04g18950 pycom12g21350 pycom12g21360
rosa_chinensis	RchiOBHm_Chr3g0453001
rosa_laevigata	RLG00000025551
rosa_multiflora	Rmu_co8146304.1_g000001 Rmu_co8342113.1_g000001 Rmu_sc0005888.1_g000003
rosa_roxburghii	Rroxscaffold_6G00430100
rosa_rugosa	Rorug02G0645100 Rorug02G0645200 Rorug02G0645300
rosa_samantha	Rh3AG047500 Rh3BG050000 Rh3CG048800 Rh3DG049600
rosa_wichuraiana	Rw3G003720

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1700
Acc36I	ACCTGC	3 cut(s)	526, 790, 1186
Acc65I	GGTACC	1 cut(s)	2305
AccB1I	GGYRCC	2 cut(s)	605, 2305
AccBSI	CCGCTC	1 cut(s)	139
AccII	CGCG	1 cut(s)	180
AciI	CCGC	5 cut(s)	139, 163, 545, 1484, 2274
AclI	AACGTT	1 cut(s)	757
AclWI	GGATC	2 cut(s)	1734, 2203
AcsI	RAATTY	5 cut(s)	394, 830, 968, 1759, 2056
AcuI	CTGAAG	4 cut(s)	455, 1032, 1244, 1556
AcyI	GRCGYC	2 cut(s)	227, 1265
AfaI	GTAC	5 cut(s)	640, 1588, 1806, 2307, 2403
AfiI	CCNNNNNNNGG	2 cut(s)	63, 109
AhdI	GACNNNNNGTC	1 cut(s)	2154
AjnI	CCWGG	5 cut(s)	117, 1175, 1623, 2184, 2291
AjuI	GAANNNNNNNTTGG	2 cut(s)	59, 91
Alw21I	GWGCWC	1 cut(s)	117
Alw26I	GTCTC	1 cut(s)	1975
AlwI	GGATC	2 cut(s)	1734, 2203
AlwNI	CAGNNNCTG	1 cut(s)	1595
AoxI	GGCC	4 cut(s)	54, 195, 1626, 1985
ApeKI	GCWGC	6 cut(s)	160, 1063, 1117, 1250, 1457, 1709
ApoI	RAATTY	5 cut(s)	394, 830, 968, 1759, 2056
ArsI	GACNNNNNNTTYG	2 cut(s)	786, 818
Asp700I	GAANNNNTTC	3 cut(s)	71, 1406, 1818
Asp718I	GGTACC	1 cut(s)	2305
AspLEI	GCGC	2 cut(s)	180, 550
AspS9I	GGNCC	6 cut(s)	55, 79, 121, 1627, 1843, 1985
AsuC2I	CCSGG	1 cut(s)	103
AsuHPI	GGTGA	2 cut(s)	1404, 1444
AsuII	TTCGAA	1 cut(s)	524
AsuNHI	GCTAGC	1 cut(s)	2014
AvaII	GGWCC	3 cut(s)	79, 121, 1843
AxyI	CCTNAGG	1 cut(s)	1428
BanI	GGYRCC	2 cut(s)	605, 2305
BanII	GRGCYC	2 cut(s)	117, 1772
BbsI	GAAGAC	4 cut(s)	1140, 1169, 1250, 1571
Bbv12I	GWGCWC	1 cut(s)	117
BbvI	GCAGC	6 cut(s)	172, 1075, 1104, 1262, 1469, 1721
BccI	CCATC	4 cut(s)	697, 835, 1175, 1673
BceAI	ACGGC	2 cut(s)	173, 593
BcgI	CGANNNNNNTGC	2 cut(s)	2140, 2174
BciT130I	CCWGG	5 cut(s)	119, 1177, 1625, 2186, 2293
BcnI	CCSGG	1 cut(s)	103
BcoDI	GTCTC	1 cut(s)	1975
BfaI	CTAG	2 cut(s)	1644, 2015
BfmI	CTRYAG	2 cut(s)	59, 1773
BfuAI	ACCTGC	3 cut(s)	526, 790, 1186
BglII	AGATCT	1 cut(s)	2227
BisI	GCNGC	9 cut(s)	161, 546, 1064, 1118, 1251, 1458, 1485, 1710, 2275
BlpI	GCTNAGC	1 cut(s)	1596
BlsI	GCNGC	9 cut(s)	162, 547, 1065, 1119, 1252, 1459, 1486, 1711, 2276
Bme1390I	CCNGG	6 cut(s)	103, 119, 1177, 1625, 2186, 2293
Bme18I	GGWCC	3 cut(s)	79, 121, 1843
BmeRI	GACNNNNNGTC	1 cut(s)	2154
BmgT120I	GGNCC	6 cut(s)	55, 79, 121, 1627, 1843, 1985
BmiI	GGNNCC	5 cut(s)	607, 1036, 1845, 2297, 2307
BmrFI	CCNGG	6 cut(s)	103, 119, 1177, 1625, 2186, 2293
BmrI	ACTGGG	1 cut(s)	1500
BmsI	GCATC	2 cut(s)	672, 1285
BmtI	GCTAGC	1 cut(s)	2018
BmuI	ACTGGG	1 cut(s)	1500
BpiI	GAAGAC	4 cut(s)	1140, 1169, 1250, 1571
BplI	GAGNNNNNCTC	2 cut(s)	128, 160
BpmI	CTGGAG	3 cut(s)	101, 134, 1915
Bpu10I	CCTNAGC	1 cut(s)	237
Bpu1102I	GCTNAGC	1 cut(s)	1596
Bpu14I	TTCGAA	1 cut(s)	524
BpuEI	CTTGAG	1 cut(s)	80
BpuMI	CCSGG	1 cut(s)	103
Bsa29I	ATCGAT	1 cut(s)	620
BsaHI	GRCGYC	2 cut(s)	227, 1265
BsaJI	CCNNGG	7 cut(s)	763, 901, 1039, 1128, 1155, 1176, 1630
BsaWI	WCCGGW	2 cut(s)	17, 2302
BsaXI	ACNNNNNCTCC	2 cut(s)	1363, 1393
Bsc4I	CCNNNNNNNGG	2 cut(s)	63, 109
Bse1I	ACTGG	2 cut(s)	1495, 2023
Bse21I	CCTNAGG	1 cut(s)	1428
Bse3DI	GCAATG	1 cut(s)	1305
BseBI	CCWGG	5 cut(s)	119, 1177, 1625, 2186, 2293
BseCI	ATCGAT	1 cut(s)	620
BseDI	CCNNGG	7 cut(s)	763, 901, 1039, 1128, 1155, 1176, 1630
BseGI	GGATG	5 cut(s)	31, 421, 1137, 1186, 1300
BseLI	CCNNNNNNNGG	2 cut(s)	63, 109
BseMI	GCAATG	1 cut(s)	1305
BseMII	CTCAG	6 cut(s)	251, 447, 1332, 1442, 1587, 1605
BseNI	ACTGG	2 cut(s)	1495, 2023
BseRI	GAGGAG	4 cut(s)	125, 188, 1387, 1710
BseXI	GCAGC	6 cut(s)	172, 1075, 1104, 1262, 1469, 1721
BseYI	CCCAGC	2 cut(s)	1276, 1846
BsgI	GTGCAG	2 cut(s)	2144, 2347
Bsh1236I	CGCG	1 cut(s)	180
Bsh1285I	CGRYCG	1 cut(s)	12
BshFI	GGCC	4 cut(s)	56, 197, 1628, 1987
BshNI	GGYRCC	2 cut(s)	605, 2305
BshVI	ATCGAT	1 cut(s)	620
BsiEI	CGRYCG	1 cut(s)	12
BsiHKAI	GWGCWC	1 cut(s)	117
BsiSI	CCGG	3 cut(s)	18, 103, 2303
BslFI	GGGAC	1 cut(s)	2161
BslI	CCNNNNNNNGG	2 cut(s)	63, 109
BsmAI	GTCTC	1 cut(s)	1975
BsmFI	GGGAC	1 cut(s)	2161
BsnI	GGCC	4 cut(s)	56, 197, 1628, 1987
Bsp119I	TTCGAA	1 cut(s)	524
Bsp1286I	GDGCHC	2 cut(s)	117, 1772
Bsp143I	GATC	8 cut(s)	9, 1228, 1726, 1825, 1957, 2208, 2227, 2384
Bsp1720I	GCTNAGC	1 cut(s)	1596
Bsp19I	CCATGG	2 cut(s)	763, 1039
BspACI	CCGC	5 cut(s)	139, 163, 545, 1484, 2274
BspANI	GGCC	4 cut(s)	56, 197, 1628, 1987
BspCNI	CTCAG	6 cut(s)	250, 448, 1333, 1441, 1588, 1606
BspDI	ATCGAT	1 cut(s)	620
BspFNI	CGCG	1 cut(s)	180
BspHI	TCATGA	2 cut(s)	507, 1203
BspLI	GGNNCC	5 cut(s)	607, 1036, 1845, 2297, 2307
BspMAI	CTGCAG	1 cut(s)	63
BspMI	ACCTGC	3 cut(s)	526, 790, 1186
BspOI	GCTAGC	1 cut(s)	2018
BspPI	GGATC	2 cut(s)	1734, 2203
BspQI	GCTCTTC	2 cut(s)	369, 789
BspT104I	TTCGAA	1 cut(s)	524
BspT107I	GGYRCC	2 cut(s)	605, 2305
BsrBI	CCGCTC	1 cut(s)	139
BsrDI	GCAATG	1 cut(s)	1305
BsrI	ACTGG	2 cut(s)	1495, 2023
BssECI	CCNNGG	7 cut(s)	763, 901, 1039, 1128, 1155, 1176, 1630
BssMI	GATC	8 cut(s)	9, 1228, 1726, 1825, 1957, 2208, 2227, 2384
BssNI	GRCGYC	2 cut(s)	227, 1265
BssT1I	CCWWGG	5 cut(s)	763, 1039, 1128, 1155, 1630
Bst2UI	CCWGG	5 cut(s)	119, 1177, 1625, 2186, 2293
Bst4CI	ACNGT	6 cut(s)	125, 638, 1330, 1363, 1552, 1591
Bst6I	CTCTTC	5 cut(s)	274, 369, 789, 1008, 1397
BstACI	GRCGYC	2 cut(s)	227, 1265
BstBI	TTCGAA	1 cut(s)	524
BstC8I	GCNNGC	2 cut(s)	1193, 2016
BstDEI	CTNAG	6 cut(s)	237, 456, 1341, 1428, 1596, 1614
BstDSI	CCRYGG	3 cut(s)	763, 901, 1039
BstF5I	GGATG	5 cut(s)	31, 421, 1137, 1186, 1300
BstFNI	CGCG	1 cut(s)	180
BstHHI	GCGC	2 cut(s)	180, 550
BstKTI	GATC	8 cut(s)	12, 1231, 1729, 1828, 1960, 2211, 2230, 2387
BstMAI	GTCTC	1 cut(s)	1975
BstMBI	GATC	8 cut(s)	9, 1228, 1726, 1825, 1957, 2208, 2227, 2384
BstMCI	CGRYCG	1 cut(s)	12
BstMWI	GCNNNNNNNGC	3 cut(s)	145, 233, 1457
BstNI	CCWGG	5 cut(s)	119, 1177, 1625, 2186, 2293
BstSCI	CCNGG	6 cut(s)	101, 117, 1175, 1623, 2184, 2291
BstSFI	CTRYAG	2 cut(s)	59, 1773
BstUI	CGCG	1 cut(s)	180
BstV1I	GCAGC	6 cut(s)	172, 1075, 1104, 1262, 1469, 1721
BstV2I	GAAGAC	4 cut(s)	1140, 1169, 1250, 1571
BstX2I	RGATCY	2 cut(s)	1726, 2227
BstXI	CCANNNNNNTGG	2 cut(s)	1631, 2192
BstYI	RGATCY	2 cut(s)	1726, 2227
Bsu15I	ATCGAT	1 cut(s)	620
Bsu36I	CCTNAGG	1 cut(s)	1428
BsuRI	GGCC	4 cut(s)	56, 197, 1628, 1987
BsuTUI	ATCGAT	1 cut(s)	620
BtgI	CCRYGG	3 cut(s)	763, 901, 1039
BtsCI	GGATG	5 cut(s)	31, 421, 1137, 1186, 1300
BtsI	GCAGTG	2 cut(s)	68, 678
BtsIMutI	CAGTG	6 cut(s)	68, 678, 1335, 1338, 1596, 2030
BveI	ACCTGC	3 cut(s)	526, 790, 1186
Cac8I	GCNNGC	2 cut(s)	1193, 2016
CaiI	CAGNNNCTG	1 cut(s)	1595
CciI	TCATGA	2 cut(s)	507, 1203
CfoI	GCGC	2 cut(s)	180, 550
Cfr13I	GGNCC	6 cut(s)	55, 79, 121, 1627, 1843, 1985
ClaI	ATCGAT	1 cut(s)	620
CseI	GACGC	2 cut(s)	216, 1254
Csp6I	GTAC	5 cut(s)	639, 1587, 1805, 2306, 2402
CspCI	CAANNNNNGTGG	2 cut(s)	1327, 1362
CviAII	CATG	8 cut(s)	508, 764, 1040, 1204, 1601, 1930, 1975, 2181
CviQI	GTAC	5 cut(s)	639, 1587, 1805, 2306, 2402
DdeI	CTNAG	6 cut(s)	237, 456, 1341, 1428, 1596, 1614
DpnI	GATC	8 cut(s)	11, 1230, 1728, 1827, 1959, 2210, 2229, 2386
DpnII	GATC	8 cut(s)	9, 1228, 1726, 1825, 1957, 2208, 2227, 2384
DraI	TTTAAA	1 cut(s)	1863
DriI	GACNNNNNGTC	1 cut(s)	2154
Eam1104I	CTCTTC	5 cut(s)	274, 369, 789, 1008, 1397
Eam1105I	GACNNNNNGTC	1 cut(s)	2154
EarI	CTCTTC	5 cut(s)	274, 369, 789, 1008, 1397
Ecl136II	GAGCTC	1 cut(s)	115
Eco130I	CCWWGG	5 cut(s)	763, 1039, 1128, 1155, 1630
Eco24I	GRGCYC	2 cut(s)	117, 1772
Eco32I	GATATC	1 cut(s)	370
Eco47I	GGWCC	3 cut(s)	79, 121, 1843
Eco53kI	GAGCTC	1 cut(s)	115
Eco57I	CTGAAG	4 cut(s)	455, 1032, 1244, 1556
Eco81I	CCTNAGG	1 cut(s)	1428
EcoICRI	GAGCTC	1 cut(s)	115
EcoO109I	RGGNCCY	1 cut(s)	1627
EcoRI	GAATTC	1 cut(s)	394
EcoRII	CCWGG	5 cut(s)	117, 1175, 1623, 2184, 2291
EcoRV	GATATC	1 cut(s)	370
EcoT14I	CCWWGG	5 cut(s)	763, 1039, 1128, 1155, 1630
EcoT38I	GRGCYC	2 cut(s)	117, 1772
ErhI	CCWWGG	5 cut(s)	763, 1039, 1128, 1155, 1630
FaeI	CATG	8 cut(s)	511, 767, 1043, 1207, 1604, 1933, 1978, 2184
FalI	AAGNNNNNCTT	2 cut(s)	2341, 2373
FaqI	GGGAC	1 cut(s)	2161
FatI	CATG	8 cut(s)	507, 763, 1039, 1203, 1600, 1929, 1974, 2180
FauI	CCCGC	1 cut(s)	156
FauNDI	CATATG	1 cut(s)	2098
Fnu4HI	GCNGC	9 cut(s)	161, 546, 1064, 1118, 1251, 1458, 1485, 1710, 2275
FokI	GGATG	5 cut(s)	38, 428, 1124, 1193, 1307
FriOI	GRGCYC	2 cut(s)	117, 1772
Fsp4HI	GCNGC	9 cut(s)	161, 546, 1064, 1118, 1251, 1458, 1485, 1710, 2275
FspBI	CTAG	2 cut(s)	1644, 2015
GlaI	GCGC	2 cut(s)	179, 549
GluI	GCNGC	9 cut(s)	161, 546, 1064, 1118, 1251, 1458, 1485, 1710, 2275
GsaI	CCCAGC	2 cut(s)	1280, 1850
GsuI	CTGGAG	3 cut(s)	101, 134, 1915
HaeIII	GGCC	4 cut(s)	56, 197, 1628, 1987
HapII	CCGG	3 cut(s)	18, 103, 2303
HgaI	GACGC	2 cut(s)	216, 1254
HhaI	GCGC	2 cut(s)	180, 550
Hin1I	GRCGYC	2 cut(s)	227, 1265
Hin1II	CATG	8 cut(s)	511, 767, 1043, 1207, 1604, 1933, 1978, 2184
Hin6I	GCGC	2 cut(s)	178, 548
HinP1I	GCGC	2 cut(s)	178, 548
HindIII	AAGCTT	1 cut(s)	2138
HpaII	CCGG	3 cut(s)	18, 103, 2303
HphI	GGTGA	2 cut(s)	1404, 1444
Hpy166II	GTNNAC	2 cut(s)	641, 1333
Hpy8I	GTNNAC	2 cut(s)	641, 1333
HpyAV	CCTTC	9 cut(s)	178, 331, 439, 462, 887, 1025, 1326, 1462, 2336
HpyCH4III	ACNGT	6 cut(s)	125, 638, 1330, 1363, 1552, 1591
HpyCH4IV	ACGT	2 cut(s)	349, 757
HpyF10VI	GCNNNNNNNGC	3 cut(s)	145, 233, 1457
HpyF3I	CTNAG	6 cut(s)	237, 456, 1341, 1428, 1596, 1614
HpySE526I	ACGT	2 cut(s)	349, 757
Hsp92I	GRCGYC	2 cut(s)	227, 1265
Hsp92II	CATG	8 cut(s)	511, 767, 1043, 1207, 1604, 1933, 1978, 2184
HspAI	GCGC	2 cut(s)	178, 548
KpnI	GGTACC	1 cut(s)	2309
Kzo9I	GATC	8 cut(s)	9, 1228, 1726, 1825, 1957, 2208, 2227, 2384
LguI	GCTCTTC	2 cut(s)	369, 789
LmnI	GCTCC	7 cut(s)	112, 120, 136, 153, 175, 1522, 1567
Lsp1109I	GCAGC	6 cut(s)	172, 1075, 1104, 1262, 1469, 1721
LweI	GCATC	2 cut(s)	672, 1285
MaeI	CTAG	2 cut(s)	1644, 2015
MaeII	ACGT	2 cut(s)	349, 757
MaeIII	GTNAC	4 cut(s)	632, 885, 1023, 1389
MalI	GATC	8 cut(s)	11, 1230, 1728, 1827, 1959, 2210, 2229, 2386
MbiI	CCGCTC	1 cut(s)	139
MboI	GATC	8 cut(s)	9, 1228, 1726, 1825, 1957, 2208, 2227, 2384
MfeI	CAATTG	2 cut(s)	2061, 2190
MflI	RGATCY	2 cut(s)	1726, 2227
MhlI	GDGCHC	2 cut(s)	117, 1772
MlyI	GAGTC	4 cut(s)	83, 762, 1585, 1808
MmeI	TCCRAC	2 cut(s)	171, 291
MroXI	GAANNNNTTC	3 cut(s)	71, 1406, 1818
MslI	CAYNNNNRTG	1 cut(s)	1788
MspA1I	CMGCKG	3 cut(s)	163, 1460, 1925
MspI	CCGG	3 cut(s)	18, 103, 2303
MspR9I	CCNGG	6 cut(s)	103, 119, 1177, 1625, 2186, 2293
MunI	CAATTG	2 cut(s)	2061, 2190
MvaI	CCWGG	5 cut(s)	119, 1177, 1625, 2186, 2293
MvnI	CGCG	1 cut(s)	180
MwoI	GCNNNNNNNGC	3 cut(s)	145, 233, 1457
NciI	CCSGG	1 cut(s)	103
NcoI	CCATGG	2 cut(s)	763, 1039
NdeI	CATATG	1 cut(s)	2098
NdeII	GATC	8 cut(s)	9, 1228, 1726, 1825, 1957, 2208, 2227, 2384
NheI	GCTAGC	1 cut(s)	2014
NlaIII	CATG	8 cut(s)	511, 767, 1043, 1207, 1604, 1933, 1978, 2184
NlaIV	GGNNCC	5 cut(s)	607, 1036, 1845, 2297, 2307
NspV	TTCGAA	1 cut(s)	524
PagI	TCATGA	2 cut(s)	507, 1203
PciSI	GCTCTTC	2 cut(s)	369, 789
PcsI	WCGNNNNNNNCGW	3 cut(s)	29, 224, 593
PdmI	GAANNNNTTC	3 cut(s)	71, 1406, 1818
PflFI	GACNNNGTC	1 cut(s)	503
PfoI	TCCNGGA	1 cut(s)	117
PkrI	GCNGC	9 cut(s)	162, 547, 1065, 1119, 1252, 1459, 1486, 1711, 2276
Ple19I	CGATCG	1 cut(s)	12
PleI	GAGTC	4 cut(s)	83, 762, 1584, 1807
PpsI	GAGTC	4 cut(s)	83, 762, 1584, 1807
PsiI	TTATAA	1 cut(s)	1700
Psp124BI	GAGCTC	1 cut(s)	117
Psp1406I	AACGTT	1 cut(s)	757
Psp6I	CCWGG	5 cut(s)	117, 1175, 1623, 2184, 2291
PspFI	CCCAGC	2 cut(s)	1276, 1846
PspGI	CCWGG	5 cut(s)	117, 1175, 1623, 2184, 2291
PspN4I	GGNNCC	5 cut(s)	607, 1036, 1845, 2297, 2307
PspPI	GGNCC	6 cut(s)	55, 79, 121, 1627, 1843, 1985
PstI	CTGCAG	1 cut(s)	63
PstNI	CAGNNNCTG	1 cut(s)	1595
PsuI	RGATCY	2 cut(s)	1726, 2227
PsyI	GACNNNGTC	1 cut(s)	503
PvuI	CGATCG	1 cut(s)	12
PvuII	CAGCTG	2 cut(s)	1460, 1925
RsaI	GTAC	5 cut(s)	640, 1588, 1806, 2307, 2403
RsaNI	GTAC	5 cut(s)	639, 1587, 1805, 2306, 2402
RseI	CAYNNNNRTG	1 cut(s)	1788
SacI	GAGCTC	1 cut(s)	117
SapI	GCTCTTC	2 cut(s)	369, 789
SatI	GCNGC	9 cut(s)	161, 546, 1064, 1118, 1251, 1458, 1485, 1710, 2275
Sau3AI	GATC	8 cut(s)	9, 1228, 1726, 1825, 1957, 2208, 2227, 2384
Sau96I	GGNCC	6 cut(s)	55, 79, 121, 1627, 1843, 1985
SchI	GAGTC	4 cut(s)	83, 762, 1585, 1808
ScrFI	CCNGG	6 cut(s)	103, 119, 1177, 1625, 2186, 2293
SduI	GDGCHC	2 cut(s)	117, 1772
SfaNI	GCATC	2 cut(s)	672, 1285
SfcI	CTRYAG	2 cut(s)	59, 1773
SfuI	TTCGAA	1 cut(s)	524
SinI	GGWCC	3 cut(s)	79, 121, 1843
SmiMI	CAYNNNNRTG	1 cut(s)	1788
SmlI	CTYRAG	1 cut(s)	95
SmoI	CTYRAG	1 cut(s)	95
SsiI	CCGC	5 cut(s)	139, 163, 545, 1484, 2274
SspMI	CTAG	2 cut(s)	1644, 2015
SstI	GAGCTC	1 cut(s)	117
StyD4I	CCNGG	6 cut(s)	101, 117, 1175, 1623, 2184, 2291
StyI	CCWWGG	5 cut(s)	763, 1039, 1128, 1155, 1630
TaaI	ACNGT	6 cut(s)	125, 638, 1330, 1363, 1552, 1591
TaiI	ACGT	2 cut(s)	352, 760
TauI	GCSGC	3 cut(s)	548, 1487, 2277
TscAI	CASTG	6 cut(s)	68, 678, 1335, 1345, 1596, 2030
TseI	GCWGC	6 cut(s)	160, 1063, 1117, 1250, 1457, 1709
TspGWI	ACGGA	5 cut(s)	398, 480, 918, 1792, 1979
TspRI	CASTG	6 cut(s)	68, 678, 1335, 1345, 1596, 2030
Tth111I	GACNNNGTC	1 cut(s)	503
VpaK11BI	GGWCC	3 cut(s)	79, 121, 1843
XapI	RAATTY	5 cut(s)	394, 830, 968, 1759, 2056
XmnI	GAANNNNTTC	3 cut(s)	71, 1406, 1818
XspI	CTAG	2 cut(s)	1644, 2015

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.