Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr12

Orthogroup ID

OG0003973

Physical Location & Seq

Genomic Coordinates Reverse (-)

30455815 .. 30460560

Gene Structure 4746 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD12G1229100.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3012 bp

ATGGAAGAGAAAGGTTTGAACTTGGAAGAATCAGAGTTGAGGCAGAGAAAGGTGGCCCTGCAATTTAAGGACCTTTTGGACTCTATTGATTCCATCCGGGACGAGATCCTCAGTCTAGTTCAAGAGGTCCAGGATAAGGAGAAGCAAAGTGGAGTTTTGCGGAGCCAAGTCAAAGCCAAATCCGAGGAATTGCATGGACTTGAGAGGTTGATTGAAGAGAAATCAAATGAGGTTGAATCACTTAAGTCCTCCTTGTGCGACTTTCAGTCATCGATCGAAGAGCACAATGAGGAGCTTTCTGCTAAGGAGAAGCGTTTGGAAGAAGTTCAGAGATTGGTTGGAGAGAAAGAGAGGGAGTATGATTCGATCAAAAAAGGGATTGAACATGGAAAAAGTAAATTGAACTGGTATGAGGAAGCTGTGGAAGAGAAGTCAAAATTGGTTGAGTCGAAGGAGGAAAAATTGAAAGGGGTTCTACGAATGTTAAAGAAGTATGATGAGGACATTGAGTTTAGAAAGATGCGATTAAATGGGATTCGGGGGTCAATTGAGGAGCGGAAGAAAGAATTTGCTTTGAAAAAAGAGCAAACTAAAGAGGCGGAAAGAAAGGTTGATCAATGTGACAGAGAGATGAAGGATAAAGAGAAGAAACTTAGTTCGATTCGGAAATCGATTGAGGAGTCCTATAAGAAAATTGAATCGAGAGAGAAAATAATTGATGCAATGAATTTGAAAGTGAAAGATTTTTCTTTGCTGAAGAAATCCATGGAGGAGTGGTCCACTAAACTCGAACTGAAAGAGCGAGAATTTGAGTCAAAAGTTAAGGATCTTACTTTCATTCAAACGAGAGTGAATGAATGCCTCAATGAGGTTCAGTTGAAAGAACAACATTTCGATTCACTTGAAAAATTGATTCGAGAAGGAAAAAAGAATTTGGATTCACAAGAAAAGTCGTTACAAGAATGCTCCAATGGTCTTGAATTAAAAGAGAGGCAGCTGGAGCAGCGGGCTAAAGAACTTGAATTGAAACAACAAGATATTGTTTCGATTCGAAATTCTGCCTCTGATAACAGTGTCATTGTTTCTTCTTCCACAAGTAATCAGTCCAGCATCAATAGGGATGGTGAAGGCTTGCAGTTACTCATGAAAGAGAGTTTGAAGAGAATTGATTCATTGAGTAGTGAATTGTCAGCTTTTCTTCAAGCGTCATCAGACCCAGCAAAATTGGTTTTGGACGCAATGGAAGGGTTTTACCCTTCAAATTCGACTGAGGACAACATGGGGCTTAATTATGATTTGATAGTTATTAGAAGGAGTTGTGTTCTTTTGCTACAGGAGTTGAAGAGGTTAACACCACAGATCAATCCTCTGGTGAGAGAAGAAGCAATGAAGTTGGCGGATGACTGGAAAGAAAAGATGACGGTGGTGGCTGAGAATAGCTTAGAGGTTTTGGGTCTTTTGTGGCTTATTACGGCATTTGAACTGACCTCAACTTATAATGCGAGAGAGCTTCAAAGTCTTCTTGCTTTAGTTTCTCAGCCAGAACATGCACCTCAACTGCGCCAGGCCCTTGGCATGACACATAAGGCATCTGATAGTGCTCTTTCTATCCCTATCAAAATTGAGGAACCAGAGCCCTCAACAGTCAGAAATGTGACTGCTTCTCCTTCTCCAATTCTTCAACTAAATGCGAATGCCCCCACCGATGCAAGGAATTCGCAGGGGCGTCCAAATGAGCATTTAAGTGGGAATGATTCAGCTAATAATGAATTGCTTGATGTTTTACGACTGCCGGTAGACCGAGAAAAAATTGTTTTGAACTTGATGCAAAAATCTCTTCATCAGTACTGGAGCAATAGAAATGTTGGTTTCATAGACAATCTCGTGAAGACCTACATTCCCATGTTGAATGAGCTGATGATAACCTCATCAAATGTTGCCCTCGATGTGAAAAAAGAGGCAACAAGCCTAGCAGTCCAGTGGAAAGCAAAAATGAGAATAGATAAGGAAAATTCATTGGAGATTTTGGGTTTTTTGCAATTTTTGGATGCATATGGATTGCTTTCGACCTTGAGTGTAGATAATACTGTGATGCTTCTTGGGATTATATGTCAGAATAAACCGGTCCTACAATTATGCCAGATACTTAGTTTTGCAGATAAGATCCCTGGTTTGATTTGGAATCTGATTGAAAGGAAGCAGCCGATTAAGGCTGTTAGATTGATTTGCACCTTCAAGTTAACTGACAGGTTCCCGCCAGTGGTACTCTTAAGTAAATATGTGGAGGATGTAAGGAAGTCTTTCCGTGCAATATTACAGGGAAAGAAAACAATTTATGAAAAGGATAATGTAATAAACAACCTAATAGCTGATCTGAGGGGTGTCCTTTGTTGCATAAAGGATCACAATCTTGAGTCCGAATACCCTTATAAGGACATTGAATTGGAAATAGTTCAGCTGGAAAGTCTAAAGGAGAATTTGAGACGCTTGCCAACGGCTATTGCCTCCACTTGTGAACAACAAGAACATGAAAGAAGGAAGAGATGCAGTACTAGTTCTGCCCCCCAGTTTCAACCACCAGAGAAACGTCAAAATAGAAGCCATCCAACAGCTGTAGCAGCTGCTGCTAGATCCTGCACATCTTTCCGTTTGCAGTTAAATCCGTCATCTCAGCTATTTGGAAATAATGGACACCCTGGGCAGTTTAGTATGGCTGCCAACCACCATGAAACTGGTGGCAGTTTTGGTTCTATGCAGTTAACTTCGTCGTCTCAGCTGTATGGAAACTATGGACACCCTTTTCAGTTTGCTATGGACCATGAAATTGGTGCTAATTTTGGTCGCATGCAGTTCAGTTCGTCATCTCAGCCGGCTGAAAATTTTGGATTCCCTCCCTTGGCTTCCAACGAGCATGGGATTGGTGGTAATTTCAGTGCTGTGCAGGATGCCGGTTTACATAACTCTAATCAGTTCTCTCCCGGCGCTCATGGTTCTGACCCTAGCGAAACCTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Frigida

Protein Analysis

1004

Amino Acids

113.88

Weight (kDa)

5.93

Isoelectric Point (pI)

50.19

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Frigida	PF07899	372 - 532	3.5e-33	Frigida-like protein
Frigida	PF07899	580 - 829	7.3e-49	Frigida-like protein

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0003973)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G27220 AT5G27220 AT5G27220 AT5G27220
fragaria_vesca	FvH4_6g04390
malus_domestica	MD04G1214300.v1.1 MD04G1214400.v1.1 MD04G1214500.v1.1 MD12G1228900.v1.1 MD12G1229100.v1.1
prunus_persica	Prupe.6G334100_v2.0.a1 Prupe.6G334200_v2.0.a1 Prupe.6G334200_v2.0.a1 Prupe.6G334200_v2.0.a1 Prupe.6G334400_v2.0.a1 Prupe.6G334500_v2.0.a1
pyrus_communis	pycom04g18950 pycom12g21350 pycom12g21360
rosa_chinensis	RchiOBHm_Chr3g0453001
rosa_laevigata	RLG00000025551
rosa_multiflora	Rmu_co8146304.1_g000001 Rmu_co8342113.1_g000001 Rmu_sc0005888.1_g000003
rosa_roxburghii	Rroxscaffold_6G00430100
rosa_rugosa	Rorug02G0645100 Rorug02G0645200 Rorug02G0645300
rosa_samantha	Rh3AG047500 Rh3BG050000 Rh3CG048800 Rh3DG049600
rosa_wichuraiana	Rw3G003720

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	1497, 2430
AccBSI	CCGCTC	1 cut(s)	556
AccI	GTMKAC	1 cut(s)	1797
AciI	CCGC	6 cut(s)	160, 556, 599, 1006, 1397, 2255
AclWI	GGATC	5 cut(s)	100, 834, 2158, 2409, 2625
AcuI	CTGAAG	1 cut(s)	776
AcyI	GRCGYC	1 cut(s)	1726
AfaI	GTAC	3 cut(s)	1847, 2265, 2551
AfiI	CCNNNNNNNGG	6 cut(s)	136, 868, 1710, 2260, 2431, 2896
AflII	CTTAAG	2 cut(s)	242, 2269
AgeI	ACCGGT	1 cut(s)	2122
AhdI	GACNNNNNGTC	1 cut(s)	265
AhlI	ACTAGT	1 cut(s)	2552
AjnI	CCWGG	4 cut(s)	129, 1563, 2167, 2695
AjuI	GAANNNNNNNTTGG	2 cut(s)	422, 454
Alw21I	GWGCWC	2 cut(s)	285, 1603
Alw26I	GTCTC	2 cut(s)	2476, 2775
AlwI	GGATC	5 cut(s)	100, 834, 2158, 2409, 2625
AlwNI	CAGNNNCTG	1 cut(s)	2624
AoxI	GGCC	2 cut(s)	54, 1566
ApeKI	GCWGC	7 cut(s)	994, 1003, 2200, 2618, 2621, 2624, 2714
AsiGI	ACCGGT	1 cut(s)	2122
Asp700I	GAANNNNTTC	4 cut(s)	324, 1168, 2300, 2451
AspLEI	GCGC	2 cut(s)	1563, 2984
AspS9I	GGNCC	7 cut(s)	55, 70, 127, 777, 1567, 2125, 2815
AsuC2I	CCSGG	2 cut(s)	98, 2979
AsuHPI	GGTGA	2 cut(s)	1136, 1384
AsuII	TTCGAA	1 cut(s)	1051
AvaII	GGWCC	5 cut(s)	70, 127, 777, 2125, 2815
BanII	GRGCYC	1 cut(s)	1638
BauI	CACGAG	1 cut(s)	1883
BbsI	GAAGAC	2 cut(s)	1511, 1895
Bbv12I	GWGCWC	2 cut(s)	285, 1603
BbvI	GCAGC	7 cut(s)	1006, 1015, 2212, 2608, 2611, 2630, 2701
BccI	CCATC	3 cut(s)	101, 1115, 2610
BceAI	ACGGC	2 cut(s)	1488, 2511
BcgI	CGANNNNNNTGC	2 cut(s)	1698, 1732
BciT130I	CCWGG	4 cut(s)	131, 1565, 2169, 2697
BclI	TGATCA	1 cut(s)	613
BcnI	CCSGG	2 cut(s)	98, 2979
BcoDI	GTCTC	2 cut(s)	2476, 2775
BcuI	ACTAGT	1 cut(s)	2552
BfaI	CTAG	6 cut(s)	116, 1970, 2553, 2628, 3000, 3010
BfmI	CTRYAG	2 cut(s)	1331, 2613
BfoI	RGCGCY	1 cut(s)	2985
BfrI	CTTAAG	2 cut(s)	242, 2269
BisI	GCNGC	7 cut(s)	995, 1004, 2201, 2619, 2622, 2625, 2715
BlsI	GCNGC	7 cut(s)	996, 1005, 2202, 2620, 2623, 2626, 2716
BmcAI	AGTACT	2 cut(s)	1847, 2551
Bme1390I	CCNGG	6 cut(s)	98, 131, 1565, 2169, 2697, 2979
Bme18I	GGWCC	5 cut(s)	70, 127, 777, 2125, 2815
BmeRI	GACNNNNNGTC	1 cut(s)	265
BmgT120I	GGNCC	7 cut(s)	55, 70, 127, 777, 1567, 2125, 2815
BmiI	GGNNCC	3 cut(s)	164, 1629, 2252
BmrFI	CCNGG	6 cut(s)	98, 131, 1565, 2169, 2697, 2979
BmrI	ACTGGG	1 cut(s)	2560
BmuI	ACTGGG	1 cut(s)	2560
BpiI	GAAGAC	2 cut(s)	1511, 1895
BpmI	CTGGAG	2 cut(s)	1019, 1870
Bpu10I	CCTNAGC	1 cut(s)	303
Bpu14I	TTCGAA	1 cut(s)	1051
BpuEI	CTTGAG	3 cut(s)	221, 2092, 2432
BpuMI	CCSGG	2 cut(s)	98, 2979
Bsa29I	ATCGAT	2 cut(s)	272, 671
BsaBI	GATNNNNATC	1 cut(s)	2406
BsaHI	GRCGYC	1 cut(s)	1726
BsaJI	CCNNGG	7 cut(s)	183, 765, 1570, 2167, 2695, 2696, 2895
BsaWI	WCCGGW	1 cut(s)	2122
BsaXI	ACNNNNNCTCC	4 cut(s)	761, 791, 1648, 1678
Bsc4I	CCNNNNNNNGG	6 cut(s)	136, 868, 1710, 2260, 2431, 2896
Bse118I	RCCGGY	4 cut(s)	1792, 2122, 2869, 2948
Bse1I	ACTGG	7 cut(s)	410, 1409, 1853, 1978, 2258, 2566, 2737
Bse3DI	GCAATG	3 cut(s)	729, 1245, 1392
Bse8I	GATNNNNATC	1 cut(s)	2406
BseBI	CCWGG	4 cut(s)	131, 1565, 2169, 2697
BseCI	ATCGAT	2 cut(s)	272, 671
BseDI	CCNNGG	7 cut(s)	183, 765, 1570, 2167, 2695, 2696, 2895
BseGI	GGATG	7 cut(s)	93, 1126, 1405, 2053, 2293, 2602, 2950
BseJI	GATNNNNATC	1 cut(s)	2406
BseLI	CCNNNNNNNGG	6 cut(s)	136, 868, 1710, 2260, 2431, 2896
BseMI	GCAATG	3 cut(s)	729, 1245, 1392
BseMII	CTCAG	8 cut(s)	124, 1260, 1422, 1550, 2366, 2684, 2786, 2879
BseNI	ACTGG	7 cut(s)	410, 1409, 1853, 1978, 2258, 2566, 2737
BseRI	GAGGAG	4 cut(s)	305, 566, 692, 785
BseXI	GCAGC	7 cut(s)	1006, 1015, 2212, 2608, 2611, 2630, 2701
BseYI	CCCAGC	1 cut(s)	1216
BsgI	GTGCAG	2 cut(s)	2620, 2960
Bsh1285I	CGRYCG	1 cut(s)	276
BshFI	GGCC	2 cut(s)	56, 1568
BshTI	ACCGGT	1 cut(s)	2122
BshVI	ATCGAT	2 cut(s)	272, 671
BsiEI	CGRYCG	1 cut(s)	276
BsiHKAI	GWGCWC	2 cut(s)	285, 1603
BsiSI	CCGG	6 cut(s)	97, 1793, 2123, 2870, 2949, 2979
BslFI	GGGAC	1 cut(s)	113
BslI	CCNNNNNNNGG	6 cut(s)	136, 868, 1710, 2260, 2431, 2896
BsmAI	GTCTC	2 cut(s)	2476, 2775
BsmBI	CGTCTC	2 cut(s)	2476, 2775
BsmFI	GGGAC	1 cut(s)	113
BsmI	GAATGC	3 cut(s)	863, 968, 1699
BsnI	GGCC	2 cut(s)	56, 1568
Bsp119I	TTCGAA	1 cut(s)	1051
Bsp1286I	GDGCHC	3 cut(s)	285, 1603, 1638
Bsp19I	CCATGG	1 cut(s)	765
BspACI	CCGC	6 cut(s)	160, 556, 599, 1006, 1397, 2255
BspANI	GGCC	2 cut(s)	56, 1568
BspCNI	CTCAG	8 cut(s)	123, 1261, 1423, 1549, 2367, 2683, 2785, 2878
BspDI	ATCGAT	2 cut(s)	272, 671
BspHI	TCATGA	1 cut(s)	1143
BspLI	GGNNCC	3 cut(s)	164, 1629, 2252
BspPI	GGATC	5 cut(s)	100, 834, 2158, 2409, 2625
BspQI	GCTCTTC	1 cut(s)	273
BspT104I	TTCGAA	1 cut(s)	1051
BspTI	CTTAAG	2 cut(s)	242, 2269
BsrBI	CCGCTC	1 cut(s)	556
BsrDI	GCAATG	3 cut(s)	729, 1245, 1392
BsrFI	RCCGGY	4 cut(s)	1792, 2122, 2869, 2948
BsrI	ACTGG	7 cut(s)	410, 1409, 1853, 1978, 2258, 2566, 2737
BssAI	RCCGGY	4 cut(s)	1792, 2122, 2869, 2948
BssECI	CCNNGG	7 cut(s)	183, 765, 1570, 2167, 2695, 2696, 2895
BssNI	GRCGYC	1 cut(s)	1726
BssSI	CACGAG	1 cut(s)	1883
BssT1I	CCWWGG	3 cut(s)	765, 1570, 2895
Bst2BI	CACGAG	1 cut(s)	1883
Bst2UI	CCWGG	4 cut(s)	131, 1565, 2169, 2697
Bst4CI	ACNGT	4 cut(s)	1073, 1423, 1645, 2089
Bst6I	CTCTTC	7 cut(s)	210, 273, 420, 1154, 1337, 1842, 2534
BstACI	GRCGYC	1 cut(s)	1726
BstAFI	CTTAAG	2 cut(s)	242, 2269
BstAPI	GCANNNNNTGC	1 cut(s)	2624
BstBI	TTCGAA	1 cut(s)	1051
BstC8I	GCNNGC	5 cut(s)	1008, 1133, 2489, 2846, 2871
BstDSI	CCRYGG	1 cut(s)	765
BstENI	CCTNNNNNAGG	1 cut(s)	866
BstF5I	GGATG	7 cut(s)	93, 1126, 1405, 2053, 2293, 2602, 2950
BstH2I	RGCGCY	1 cut(s)	2985
BstHHI	GCGC	2 cut(s)	1563, 2984
BstMAI	GTCTC	2 cut(s)	2476, 2775
BstMCI	CGRYCG	1 cut(s)	276
BstMWI	GCNNNNNNNGC	4 cut(s)	1000, 1003, 2618, 2624
BstNI	CCWGG	4 cut(s)	131, 1565, 2169, 2697
BstNSI	RCATGY	2 cut(s)	1550, 2848
BstSCI	CCNGG	6 cut(s)	96, 129, 1563, 2167, 2695, 2977
BstSFI	CTRYAG	2 cut(s)	1331, 2613
BstV1I	GCAGC	7 cut(s)	1006, 1015, 2212, 2608, 2611, 2630, 2701
BstV2I	GAAGAC	2 cut(s)	1511, 1895
BstX2I	RGATCY	4 cut(s)	105, 826, 2163, 2630
BstXI	CCANNNNNNTGG	3 cut(s)	1571, 2732, 2825
BstYI	RGATCY	4 cut(s)	105, 826, 2163, 2630
Bsu15I	ATCGAT	2 cut(s)	272, 671
BsuRI	GGCC	2 cut(s)	56, 1568
BsuTUI	ATCGAT	2 cut(s)	272, 671
BtgI	CCRYGG	1 cut(s)	765
BtsCI	GGATG	7 cut(s)	93, 1126, 1405, 2053, 2293, 2602, 2950
BtsIMutI	CAGTG	4 cut(s)	1078, 1985, 2265, 2938
Cac8I	GCNNGC	5 cut(s)	1008, 1133, 2489, 2846, 2871
CaiI	CAGNNNCTG	1 cut(s)	2624
CciI	TCATGA	1 cut(s)	1143
CfoI	GCGC	2 cut(s)	1563, 2984
Cfr10I	RCCGGY	4 cut(s)	1792, 2122, 2869, 2948
Cfr13I	GGNCC	7 cut(s)	55, 70, 127, 777, 1567, 2125, 2815
ClaI	ATCGAT	2 cut(s)	272, 671
CseI	GACGC	4 cut(s)	1194, 1244, 1715, 2493
Csp6I	GTAC	3 cut(s)	1846, 2264, 2550
CspAI	ACCGGT	1 cut(s)	2122
CviQI	GTAC	3 cut(s)	1846, 2264, 2550
DriI	GACNNNNNGTC	1 cut(s)	265
Eam1104I	CTCTTC	7 cut(s)	210, 273, 420, 1154, 1337, 1842, 2534
Eam1105I	GACNNNNNGTC	1 cut(s)	265
EarI	CTCTTC	7 cut(s)	210, 273, 420, 1154, 1337, 1842, 2534
EciI	GGCGGA	2 cut(s)	614, 1412
Eco130I	CCWWGG	3 cut(s)	765, 1570, 2895
Eco24I	GRGCYC	1 cut(s)	1638
Eco47I	GGWCC	5 cut(s)	70, 127, 777, 2125, 2815
Eco57I	CTGAAG	1 cut(s)	776
EcoNI	CCTNNNNNAGG	1 cut(s)	866
EcoO109I	RGGNCCY	2 cut(s)	70, 1567
EcoRI	GAATTC	1 cut(s)	1714
EcoRII	CCWGG	4 cut(s)	129, 1563, 2167, 2695
EcoT14I	CCWWGG	3 cut(s)	765, 1570, 2895
EcoT22I	ATGCAT	1 cut(s)	2053
EcoT38I	GRGCYC	1 cut(s)	1638
ErhI	CCWWGG	3 cut(s)	765, 1570, 2895
Esp3I	CGTCTC	2 cut(s)	2476, 2775
FalI	AAGNNNNNCTT	4 cut(s)	236, 268, 2284, 2316
FaqI	GGGAC	1 cut(s)	113
FauI	CCCGC	2 cut(s)	999, 2262
FauNDI	CATATG	1 cut(s)	2053
FbaI	TGATCA	1 cut(s)	613
FblI	GTMKAC	1 cut(s)	1797
Fnu4HI	GCNGC	7 cut(s)	995, 1004, 2201, 2619, 2622, 2625, 2715
FokI	GGATG	7 cut(s)	80, 1133, 1412, 2060, 2300, 2589, 2957
FriOI	GRGCYC	1 cut(s)	1638
Fsp4HI	GCNGC	7 cut(s)	995, 1004, 2201, 2619, 2622, 2625, 2715
FspBI	CTAG	6 cut(s)	116, 1970, 2553, 2628, 3000, 3010
GlaI	GCGC	2 cut(s)	1562, 2983
GluI	GCNGC	7 cut(s)	995, 1004, 2201, 2619, 2622, 2625, 2715
GsaI	CCCAGC	1 cut(s)	1220
GsuI	CTGGAG	2 cut(s)	1019, 1870
HaeII	RGCGCY	1 cut(s)	2985
HaeIII	GGCC	2 cut(s)	56, 1568
HapII	CCGG	6 cut(s)	97, 1793, 2123, 2870, 2949, 2979
HgaI	GACGC	4 cut(s)	1194, 1244, 1715, 2493
HhaI	GCGC	2 cut(s)	1563, 2984
Hin1I	GRCGYC	1 cut(s)	1726
Hin6I	GCGC	2 cut(s)	1561, 2982
HinP1I	GCGC	2 cut(s)	1561, 2982
HincII	GTYRAC	3 cut(s)	1350, 2241, 2760
HindII	GTYRAC	3 cut(s)	1350, 2241, 2760
HpaI	GTTAAC	3 cut(s)	1350, 2241, 2760
HpaII	CCGG	6 cut(s)	97, 1793, 2123, 2870, 2949, 2979
HphI	GGTGA	2 cut(s)	1136, 1384
Hpy166II	GTNNAC	7 cut(s)	780, 1350, 1798, 2241, 2516, 2760, 2954
Hpy188III	TCNNGA	7 cut(s)	122, 702, 917, 977, 1144, 1885, 2411
Hpy8I	GTNNAC	7 cut(s)	780, 1350, 1798, 2241, 2516, 2760, 2954
Hpy99I	CGWCG	1 cut(s)	2770
HpyCH4III	ACNGT	4 cut(s)	1073, 1423, 1645, 2089
HpyCH4IV	ACGT	1 cut(s)	2587
HpyF10VI	GCNNNNNNNGC	4 cut(s)	1000, 1003, 2618, 2624
HpySE526I	ACGT	1 cut(s)	2587
Hsp92I	GRCGYC	1 cut(s)	1726
HspAI	GCGC	2 cut(s)	1561, 2982
KroI	GCCGGC	1 cut(s)	2869
KroNI	GCCGGC	1 cut(s)	2871
Ksp22I	TGATCA	1 cut(s)	613
KspAI	GTTAAC	3 cut(s)	1350, 2241, 2760
LguI	GCTCTTC	1 cut(s)	273
LmnI	GCTCC	6 cut(s)	162, 292, 553, 971, 1000, 1851
Lsp1109I	GCAGC	7 cut(s)	1006, 1015, 2212, 2608, 2611, 2630, 2701
MaeI	CTAG	6 cut(s)	116, 1970, 2553, 2628, 3000, 3010
MaeII	ACGT	1 cut(s)	2587
MaeIII	GTNAC	4 cut(s)	620, 954, 1137, 1654
MbiI	CCGCTC	1 cut(s)	556
MfeI	CAATTG	1 cut(s)	546
MflI	RGATCY	4 cut(s)	105, 826, 2163, 2630
MhlI	GDGCHC	3 cut(s)	285, 1603, 1638
MlyI	GAGTC	5 cut(s)	74, 455, 689, 821, 2423
MmeI	TCCRAC	3 cut(s)	319, 2630, 2928
Mph1103I	ATGCAT	1 cut(s)	2053
MroNI	GCCGGC	1 cut(s)	2869
MroXI	GAANNNNTTC	4 cut(s)	324, 1168, 2300, 2451
MslI	CAYNNNNRTG	2 cut(s)	1743, 1901
MspA1I	CMGCKG	6 cut(s)	997, 1006, 2458, 2612, 2621, 2776
MspCI	CTTAAG	2 cut(s)	242, 2269
MspI	CCGG	6 cut(s)	97, 1793, 2123, 2870, 2949, 2979
MspR9I	CCNGG	6 cut(s)	98, 131, 1565, 2169, 2697, 2979
MunI	CAATTG	1 cut(s)	546
Mva1269I	GAATGC	3 cut(s)	863, 968, 1699
MvaI	CCWGG	4 cut(s)	131, 1565, 2169, 2697
MwoI	GCNNNNNNNGC	4 cut(s)	1000, 1003, 2618, 2624
NaeI	GCCGGC	1 cut(s)	2871
NciI	CCSGG	2 cut(s)	98, 2979
NcoI	CCATGG	1 cut(s)	765
NdeI	CATATG	1 cut(s)	2053
NgoMIV	GCCGGC	1 cut(s)	2869
NlaIV	GGNNCC	3 cut(s)	164, 1629, 2252
NmuCI	GTSAC	2 cut(s)	620, 1654
NsiI	ATGCAT	1 cut(s)	2053
NspI	RCATGY	2 cut(s)	1550, 2848
NspV	TTCGAA	1 cut(s)	1051
PaeI	GCATGC	1 cut(s)	2848
PagI	TCATGA	1 cut(s)	1143
PasI	CCCWGGG	1 cut(s)	2696
PciSI	GCTCTTC	1 cut(s)	273
PctI	GAATGC	3 cut(s)	863, 968, 1699
PdiI	GCCGGC	1 cut(s)	2871
PdmI	GAANNNNTTC	4 cut(s)	324, 1168, 2300, 2451
PfoI	TCCNGGA	2 cut(s)	96, 129
PinAI	ACCGGT	1 cut(s)	2122
PkrI	GCNGC	7 cut(s)	996, 1005, 2202, 2620, 2623, 2626, 2716
Ple19I	CGATCG	1 cut(s)	276
PleI	GAGTC	5 cut(s)	74, 454, 688, 820, 2422
PpsI	GAGTC	5 cut(s)	74, 454, 688, 820, 2422
PpuMI	RGGWCCY	1 cut(s)	70
PsiI	TTATAA	2 cut(s)	1497, 2430
Psp5II	RGGWCCY	1 cut(s)	70
Psp6I	CCWGG	4 cut(s)	129, 1563, 2167, 2695
PspFI	CCCAGC	1 cut(s)	1216
PspGI	CCWGG	4 cut(s)	129, 1563, 2167, 2695
PspN4I	GGNNCC	3 cut(s)	164, 1629, 2252
PspPI	GGNCC	7 cut(s)	55, 70, 127, 777, 1567, 2125, 2815
PspPPI	RGGWCCY	1 cut(s)	70
PstNI	CAGNNNCTG	1 cut(s)	2624
PsuI	RGATCY	4 cut(s)	105, 826, 2163, 2630
PvuI	CGATCG	1 cut(s)	276
PvuII	CAGCTG	5 cut(s)	997, 2458, 2612, 2621, 2776
RsaI	GTAC	3 cut(s)	1847, 2265, 2551
RsaNI	GTAC	3 cut(s)	1846, 2264, 2550
RseI	CAYNNNNRTG	2 cut(s)	1743, 1901
SapI	GCTCTTC	1 cut(s)	273
SatI	GCNGC	7 cut(s)	995, 1004, 2201, 2619, 2622, 2625, 2715
Sau96I	GGNCC	7 cut(s)	55, 70, 127, 777, 1567, 2125, 2815
ScaI	AGTACT	2 cut(s)	1847, 2551
SchI	GAGTC	5 cut(s)	74, 455, 689, 821, 2423
ScrFI	CCNGG	6 cut(s)	98, 131, 1565, 2169, 2697, 2979
SduI	GDGCHC	3 cut(s)	285, 1603, 1638
SfcI	CTRYAG	2 cut(s)	1331, 2613
SfuI	TTCGAA	1 cut(s)	1051
SinI	GGWCC	5 cut(s)	70, 127, 777, 2125, 2815
SmiMI	CAYNNNNRTG	2 cut(s)	1743, 1901
SmlI	CTYRAG	5 cut(s)	200, 242, 2071, 2269, 2411
SmoI	CTYRAG	5 cut(s)	200, 242, 2071, 2269, 2411
SpeI	ACTAGT	1 cut(s)	2552
SphI	GCATGC	1 cut(s)	2848
SsiI	CCGC	6 cut(s)	160, 556, 599, 1006, 1397, 2255
SspI	AATATT	1 cut(s)	2313
SspMI	CTAG	6 cut(s)	116, 1970, 2553, 2628, 3000, 3010
StyD4I	CCNGG	6 cut(s)	96, 129, 1563, 2167, 2695, 2977
StyI	CCWWGG	3 cut(s)	765, 1570, 2895
TaaI	ACNGT	4 cut(s)	1073, 1423, 1645, 2089
TaiI	ACGT	1 cut(s)	2590
TaqII	GACCGA	1 cut(s)	1815
TatI	WGTACW	2 cut(s)	1845, 2549
TscAI	CASTG	4 cut(s)	1078, 1985, 2265, 2938
TseFI	GTSAC	2 cut(s)	620, 1654
TseI	GCWGC	7 cut(s)	994, 1003, 2200, 2618, 2621, 2624, 2714
Tsp45I	GTSAC	2 cut(s)	620, 1654
TspGWI	ACGGA	3 cut(s)	2294, 2636, 2652
TspRI	CASTG	4 cut(s)	1078, 1985, 2265, 2938
Vha464I	CTTAAG	2 cut(s)	242, 2269
VpaK11BI	GGWCC	5 cut(s)	70, 127, 777, 2125, 2815
XagI	CCTNNNNNAGG	1 cut(s)	866
XceI	RCATGY	2 cut(s)	1550, 2848
XcmI	CCANNNNNNNNNTGG	2 cut(s)	2729, 2732
XmiI	GTMKAC	1 cut(s)	1797
XmnI	GAANNNNTTC	4 cut(s)	324, 1168, 2300, 2451
XspI	CTAG	6 cut(s)	116, 1970, 2553, 2628, 3000, 3010
ZrmI	AGTACT	2 cut(s)	1847, 2551
Zsp2I	ATGCAT	1 cut(s)	2053

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.