MD04G1217700.v1.1

Serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr04
OG0004456
Physical Location & Seq
Reverse (-)
30024670 .. 30036916
12247 bp
Loading structure...
UTR
Exon/CDS
Intron
MD04G1217700.v1.1.491

Sequence Viewer

Length: 2880 bp
ATGAAGCACATTTTCAAGAAGCTTCACATAGGAAGCAACCACGACCCGACCCGATCGAACGAGGCCGCGGCGGCTTCCCCGTCCACCGTGGCCTCGCCCTCGCTCGGTTCCGATCACCATACGGTGTCCGATCAGACGTCAGGGAACGCTCCGGCGAGTCCTTCGTCGTCTTCGCCGACGCCGGCGGGGACCTTGCCGGCTGGGGGAGGTGGCTCTGTGGAGTCGACGATGACTGTGAACCGACCGGACTATATGATGTCGGAAGAGGAGTTTCAAGTCCAGCTTGCTTTAGCGATCAGCGCTTCGAATTCGGACTATCGGGATGATCCGGAGAAGGATCAGATCCGGGCGGCGATGCTGCTGAGCTTGGGCCGCAATCGGATCGAGTCTTCGAGAGATAAGGTTGAGGCGGCGGCGGAGACTTTGTCGCGGCACTATTGGGAGTTCAATGCGCTAGACTATGAGGAGAAAGTGGTTGATGGATTTTATGATGTATATGGACTTTCCCCAGATTCAGCAATTCAAGGAAAAATGCCATCTCTCACAAATCTTGAAGCAAACCTTGGGAGTTCTGGATTTGAAGTTTCAGTAGTAAATCGAAAAGTTGATCCTGTCTTGGAAGAGTTGGTGCAAATTGCACAGTGTATTGCTTTAGATTGTCCTGTCACCAATGTTGGTGTTTTAGTTCAGAGGCTTGCTGAACTCGTTAGCGAACGTATGGGTGGGCCTGTAAAGGATGCAAATGTTGTATTAGCGAGATGGATGGAAAGAAGCACAGAATTGAGGACATCAATGCAAACTAGTGTACTGCCTATTGGATCCATTACTATTGGTCTCTCCCGACATCGGGCTTTGCTTTTCAAGGTACTAGCTGACAATATCGAGATGCCCTGTAGACTGCTTAAGGGAGTTCATTACACTGGTGCTGAGGACGGTGCAGTTAATGTAATAAAGTTGGAGGATGACAGGGAGTTTTTGGTGGATCTGATGGCTGATCCTGGAACACTCGTGCCAACTGACATCCAAAGTTCAAAGGACACTACCTTTAAGCCATATAATCCAAACTTGAACAAACTTCCTACTTTACATTCTTATGTCGACACAGATATCGCTTACCCAGGACCAAAGCCATTACATGGAGAGGGAAGCAGCCAAAACTCTGCACTTGAAAGTAGTTCAGGTCTGGAGAGAAGACCAAGTTCAGGGAAAGGAGAATCATTTCCAACATTCTCAGGTGCAAGTAGTGACAGTGGAGTTGGTTCTTCTGGAATACCGAATAGAACAACTCAGTTAGATCAGCTTTCCTCATCAGCCTTCGAAAATTCAAAGTACAACAGAGGGAATCGTGGGGCTCATGCAGGTGATGGTGGTATGAGGATGAATGTAAATGTAGTTCCATATACTCAAAACAACTCTGAGGACCCAAAGAACCTTTTTGCTGATCTTAACCCATTCCAAATAAAAGGACCCGGGAAAGCTTCTATGTATAACAAACCGGTGGAGAATAAAGTTGAAGAGCTTCAGAAAAAACAAAATAATCTTGCTTCTGGTCGACCTCCTGCATCATTGATGTGGAAAAATAAATATGCTTTCAATGAAGTCCCTAAAAGAAAGGAGTATGATGGCATCTTCCCAAAAATAAATCGTGAGCCTAATGGTTACAATTCATCATCTTCAGCTTCGACCAGCTCTACTGTTGCTGAACAGATCAATCCTGGTGGTTTCGGATCAACTGCTCGTTCAAATATGTTTGATAGAGATGGCGATGCCAAAAGTTTTAAGGGGGAACAACCCAGGGCTCCGAACTATTTACAGAACAACACAGTAGATGCTACATATCAGAACAATGAAATTGGTTCCCATGATCCTAGAAAGTTCACGCATGACAGATTTATGGAATCCAATTTGAAACTGAAGGATCCAGAAAGTTGTAACTCGTCATTTGATTCCATTTCAAGCAGGGTTGAATATCATTCTAGCTCATCATTTGAGGATGTTGACGTAGGTGAATGTGAAATTCCATGGGAAGACCTGGTTATTGGAGAAAGGATTGGACTAGGTTCATATGGAGAGGTATACCATGCTGATTGGCATGGCACGGAAGTTGCTGTGAAGAAGTTCCTAGATCAGGACTTCTCGGGTGCTGCTTTGGCTGAGTTCAAAAGAGAAGTACGAATAATGCGGAGATTGCGTCATCCAAACGTTGTGCTTTTCATGGGGGCTGTTACACGTCCTCCAAACCTCTCTATCATTACAGAGTTCCTTCCAAGGGGAAGTCTATATCGGATTATCCATCGTCCTCATTGTCAAATTGATGAGAAACGTAGAATAAAGATGGCTCTTGATGTGGCAAGGGGTATGAATTGCTTGCATGCCAGTACACCGACGATTGTCCACCGGGATTTGAAGTCTCCAAATCTGCTGGTTGATAAGAACTGGAATGTGAAGGTTGGTGATTTTGGATTGTCGCGCTTGAAACACAACACTTTCTTGTCGTCCAAATCAACTGCTGGAACGCCTGAGTGGATGTCACCTGAAGTTCTCCGCAACGAAAACTCAAATGAAAAGTGCGACGTATTTAGCTTTGGAGTCATTTTGTGGGAGCTTGCAACTCTAAAATTGCCTTGGAGTGGCATGAACCCAATGCAAGTCGTCGGTGCAGTGGGTTTCCAGAATCGTCGCCTTGAGATCCCCAAGGAACTTGATCCCCATGTTGCAAGGATAATTTGGGAATGTTGGCAGACCGAACCAAACTTGCGCCCCTCGTTCTCAGAGCTCACGGTAGCTCTCAAGCCCTTGCAGCGGCTTGTCATTCCGTCGAATCTGGACCACCCGAGCTCACCTCTCCGGCAAGAAATCTCAGTAAATTATACACCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000165 GO:0000186 GO:0001101 GO:0001932 GO:0001934 GO:0002229 GO:0002239 GO:0002831 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0004709 GO:0004712 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005768 GO:0005769 GO:0005783 GO:0005794 GO:0005798 GO:0005802 GO:0005829 GO:0005886 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0006952 GO:0007154 GO:0007165 GO:0008150 GO:0008152 GO:0008219 GO:0009414 GO:0009415 GO:0009605 GO:0009607 GO:0009617 GO:0009620 GO:0009628 GO:0009719 GO:0009723 GO:0009725 GO:0009787 GO:0009788 GO:0009893 GO:0009966 GO:0009967 GO:0009968 GO:0009987 GO:0010033 GO:0010035 GO:0010562 GO:0010604 GO:0010646 GO:0010647 GO:0010648 GO:0012505 GO:0012506 GO:0012510 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019220 GO:0019222 GO:0019538 GO:0023014 GO:0023051 GO:0023052 GO:0023056 GO:0023057 GO:0030133 GO:0030135 GO:0030136 GO:0030140 GO:0030658 GO:0030659 GO:0030660 GO:0030662 GO:0030665 GO:0031090 GO:0031323 GO:0031325 GO:0031347 GO:0031399 GO:0031401 GO:0031410 GO:0031982 GO:0031984 GO:0032101 GO:0032147 GO:0032268 GO:0032270 GO:0033674 GO:0035556 GO:0036211 GO:0042221 GO:0042325 GO:0042327 GO:0043085 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043405 GO:0043406 GO:0043408 GO:0043410 GO:0043412 GO:0043549 GO:0043900 GO:0044093 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044431 GO:0044433 GO:0044444 GO:0044446 GO:0044464 GO:0045859 GO:0045860 GO:0045937 GO:0046777 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048583 GO:0048584 GO:0048585 GO:0050789 GO:0050790 GO:0050794 GO:0050896 GO:0051171 GO:0051173 GO:0051174 GO:0051246 GO:0051247 GO:0051338 GO:0051347 GO:0051704 GO:0051707 GO:0051716 GO:0060255 GO:0065007 GO:0065009 GO:0071704 GO:0071900 GO:0071902 GO:0071944 GO:0080090 GO:0080134 GO:0097708 GO:0098542 GO:0098588 GO:0098791 GO:0098805 GO:0140096 GO:1900150 GO:1900424 GO:1901419 GO:1901420 GO:1901564 GO:1901700 GO:1902531 GO:1902533 GO:1905957 GO:1905958 GO:2000031 GO:2001023 GO:2001038
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EDR1 Pkinase_Tyr

Protein Analysis

960

Amino Acids

105.77

Weight (kDa)

6.0

Isoelectric Point (pI)

46.44

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EDR1_CTR1_ARMC3_pept PF14381 139 - 337 1.8e-73 EDR1/CTR1/ARMC3, peptidase-like
PK_Tyr_Ser-Thr PF07714 679 - 928 1.9e-62 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 680 - 926 1.4e-49 Protein kinase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1349
AatII GACGTC 1 cut(s) 140
Acc36I ACCTGC 1 cut(s) 1349
AccB7I CCANNNNNTGG 1 cut(s) 1136
AccI GTMKAC 5 cut(s) 224, 895, 1098, 1552, 2076
AccII CGCG 3 cut(s) 68, 430, 2470
AccIII TCCGGA 1 cut(s) 328
AclI AACGTT 1 cut(s) 2202
AcsI RAATTY 3 cut(s) 307, 1321, 2016
AcuI CTGAAG 4 cut(s) 1505, 1659, 1934, 2556
AcyI GRCGYC 2 cut(s) 137, 179
AfaI GTAC 5 cut(s) 807, 867, 1331, 2172, 2380
AfeI AGCGCT 1 cut(s) 301
AflII CTTAAG 1 cut(s) 902
AflIII ACRYGT 1 cut(s) 2228
AgeI ACCGGT 1 cut(s) 1495
AhlI ACTAGT 1 cut(s) 800
AjiI CACGTC 1 cut(s) 2231
AjnI CCWGG 5 cut(s) 997, 1117, 1714, 1793, 2031
AjuI GAANNNNNNNTTGG 2 cut(s) 546, 578
Alw21I GWGCWC 2 cut(s) 2778, 2840
Alw26I GTCTC 3 cut(s) 413, 839, 2415
Ama87I CYCGRG 3 cut(s) 1469, 2137, 2833
Aor13HI TCCGGA 1 cut(s) 328
Aor51HI AGCGCT 1 cut(s) 301
AoxI GGCC 4 cut(s) 63, 90, 370, 725
ApeKI GCWGC 4 cut(s) 358, 1149, 2144, 2800
ApoI RAATTY 3 cut(s) 307, 1321, 2016
AsiGI ACCGGT 1 cut(s) 1495
Asp700I GAANNNNTTC 3 cut(s) 1218, 1518, 2117
AspLEI GCGC 4 cut(s) 302, 454, 2472, 2760
AspS9I GGNCC 7 cut(s) 189, 370, 725, 1121, 1420, 1466, 2827
AsuC2I CCSGG 4 cut(s) 347, 1470, 1471, 2399
AsuHPI GGTGA 7 cut(s) 107, 658, 1373, 2018, 2465, 2523, 2832
AsuII TTCGAA 2 cut(s) 305, 1317
AvaI CYCGRG 3 cut(s) 1469, 2137, 2833
AvaII GGWCC 5 cut(s) 189, 1121, 1420, 1466, 2827
BamHI GGATCC 2 cut(s) 818, 1918
BanII GRGCYC 4 cut(s) 1354, 1801, 2778, 2840
BauI CACGAG 1 cut(s) 1007
BbsI GAAGAC 4 cut(s) 162, 381, 1198, 2034
Bbv12I GWGCWC 2 cut(s) 2778, 2840
BbvCI CCTCAGC 1 cut(s) 927
BbvI GCAGC 4 cut(s) 345, 1161, 2131, 2812
BcgI CGANNNNNNTGC 2 cut(s) 364, 398
BciT130I CCWGG 5 cut(s) 999, 1119, 1716, 1795, 2033
BcnI CCSGG 4 cut(s) 347, 1470, 1471, 2399
BcoDI GTCTC 3 cut(s) 413, 839, 2415
BcuI ACTAGT 1 cut(s) 800
BfaI CTAG 7 cut(s) 455, 801, 869, 1869, 1977, 2057, 2123
BfmI CTRYAG 1 cut(s) 892
BfoI RGCGCY 1 cut(s) 303
BfrI CTTAAG 1 cut(s) 902
BfuAI ACCTGC 1 cut(s) 1349
BglI GCCNNNNNGGC 1 cut(s) 71
BlpI GCTNAGC 1 cut(s) 362
Bme1390I CCNGG 9 cut(s) 347, 999, 1119, 1470, 1471, 1716, 1795, 2033, 2399
Bme18I GGWCC 5 cut(s) 189, 1121, 1420, 1466, 2827
BmeT110I CYCGRG 3 cut(s) 1469, 2137, 2833
BmgBI CACGTC 1 cut(s) 2231
BmgT120I GGNCC 7 cut(s) 189, 370, 725, 1121, 1420, 1466, 2827
BmiI GGNNCC 8 cut(s) 109, 190, 820, 1422, 1468, 1800, 1858, 1920
BmrFI CCNGG 9 cut(s) 347, 999, 1119, 1470, 1471, 1716, 1795, 2033, 2399
BmsI GCATC 7 cut(s) 345, 727, 876, 1571, 1635, 1756, 1819
BoxI GACNNNNGTC 1 cut(s) 2390
BpiI GAAGAC 4 cut(s) 162, 381, 1198, 2034
BplI GAGNNNNNCTC 2 cut(s) 2827, 2859
BpmI CTGGAG 1 cut(s) 1205
Bpu10I CCTNAGC 1 cut(s) 927
Bpu1102I GCTNAGC 1 cut(s) 362
Bpu14I TTCGAA 2 cut(s) 305, 1317
BpuEI CTTGAG 2 cut(s) 2705, 2774
BpuMI CCSGG 4 cut(s) 347, 1470, 1471, 2399
BsaHI GRCGYC 2 cut(s) 137, 179
BsaI GGTCTC 1 cut(s) 839
BsaWI WCCGGW 3 cut(s) 244, 328, 1495
BsaXI ACNNNNNCTCC 6 cut(s) 410, 440, 2176, 2206, 2218, 2248
Bse118I RCCGGY 3 cut(s) 181, 196, 1495
Bse1I ACTGG 3 cut(s) 925, 2376, 2441
BseAI TCCGGA 1 cut(s) 328
BseBI CCWGG 5 cut(s) 999, 1119, 1716, 1795, 2033
BseGI GGATG 9 cut(s) 328, 742, 768, 967, 1020, 1383, 1999, 2194, 2532
BseMII CTCAG 9 cut(s) 353, 918, 1245, 1301, 1407, 2145, 2511, 2784, 2874
BseNI ACTGG 3 cut(s) 925, 2376, 2441
BseRI GAGGAG 2 cut(s) 281, 479
BseXI GCAGC 4 cut(s) 345, 1161, 2131, 2812
BseYI CCCAGC 1 cut(s) 200
BsgI GTGCAG 3 cut(s) 957, 1146, 2679
Bsh1236I CGCG 3 cut(s) 68, 430, 2470
Bsh1285I CGRYCG 2 cut(s) 56, 245
BshFI GGCC 4 cut(s) 65, 92, 372, 727
BshTI ACCGGT 1 cut(s) 1495
BsiEI CGRYCG 2 cut(s) 56, 245
BsiHKAI GWGCWC 2 cut(s) 2778, 2840
BsiHKCI CYCGRG 3 cut(s) 1469, 2137, 2833
BslFI GGGAC 2 cut(s) 202, 1586
BsmAI GTCTC 3 cut(s) 413, 839, 2415
BsmFI GGGAC 2 cut(s) 202, 1586
BsnI GGCC 4 cut(s) 65, 92, 372, 727
Bso31I GGTCTC 1 cut(s) 839
BsoBI CYCGRG 3 cut(s) 1469, 2137, 2833
Bsp119I TTCGAA 2 cut(s) 305, 1317
Bsp1286I GDGCHC 4 cut(s) 1354, 1801, 2778, 2840
Bsp13I TCCGGA 1 cut(s) 328
Bsp1720I GCTNAGC 1 cut(s) 362
Bsp19I CCATGG 1 cut(s) 2021
BspANI GGCC 4 cut(s) 65, 92, 372, 727
BspCNI CTCAG 9 cut(s) 354, 919, 1244, 1300, 1408, 2146, 2512, 2783, 2873
BspEI TCCGGA 1 cut(s) 328
BspFNI CGCG 3 cut(s) 68, 430, 2470
BspLI GGNNCC 8 cut(s) 109, 190, 820, 1422, 1468, 1800, 1858, 1920
BspMI ACCTGC 1 cut(s) 1349
BspQI GCTCTTC 1 cut(s) 1509
BspT104I TTCGAA 2 cut(s) 305, 1317
BspTI CTTAAG 1 cut(s) 902
BspTNI GGTCTC 1 cut(s) 839
BsrFI RCCGGY 3 cut(s) 181, 196, 1495
BsrI ACTGG 3 cut(s) 925, 2376, 2441
BssAI RCCGGY 3 cut(s) 181, 196, 1495
BssNAI GTATAC 1 cut(s) 2077
BssNI GRCGYC 2 cut(s) 137, 179
BssSI CACGAG 1 cut(s) 1007
BssT1I CCWWGG 5 cut(s) 562, 2021, 2267, 2624, 2694
Bst1107I GTATAC 1 cut(s) 2077
Bst2BI CACGAG 1 cut(s) 1007
Bst2UI CCWGG 5 cut(s) 999, 1119, 1716, 1795, 2033
Bst4CI ACNGT 9 cut(s) 88, 124, 235, 642, 935, 1250, 1696, 1825, 2782
Bst6I CTCTTC 3 cut(s) 258, 615, 1509
BstACI GRCGYC 2 cut(s) 137, 179
BstAFI CTTAAG 1 cut(s) 902
BstBI TTCGAA 2 cut(s) 305, 1317
BstC8I GCNNGC 7 cut(s) 183, 198, 285, 696, 2369, 2373, 2607
BstDEI CTNAG 9 cut(s) 362, 927, 1231, 1287, 1416, 2154, 2520, 2770, 2860
BstDSI CCRYGG 3 cut(s) 66, 87, 2021
BstENI CCTNNNNNAGG 1 cut(s) 2126
BstF5I GGATG 9 cut(s) 328, 742, 768, 967, 1020, 1383, 1999, 2194, 2532
BstFNI CGCG 3 cut(s) 68, 430, 2470
BstH2I RGCGCY 1 cut(s) 303
BstHHI GCGC 4 cut(s) 302, 454, 2472, 2760
BstMAI GTCTC 3 cut(s) 413, 839, 2415
BstMCI CGRYCG 2 cut(s) 56, 245
BstMWI GCNNNNNNNGC 6 cut(s) 71, 299, 372, 2150, 2188, 2800
BstNI CCWGG 5 cut(s) 999, 1119, 1716, 1795, 2033
BstNSI RCATGY 1 cut(s) 2375
BstPAI GACNNNNGTC 1 cut(s) 2390
BstSCI CCNGG 9 cut(s) 345, 997, 1117, 1468, 1469, 1714, 1793, 2031, 2397
BstSFI CTRYAG 1 cut(s) 892
BstUI CGCG 3 cut(s) 68, 430, 2470
BstV1I GCAGC 4 cut(s) 345, 1161, 2131, 2812
BstV2I GAAGAC 4 cut(s) 162, 381, 1198, 2034
BstX2I RGATCY 5 cut(s) 342, 818, 982, 1918, 2688
BstYI RGATCY 5 cut(s) 342, 818, 982, 1918, 2688
BstZ17I GTATAC 1 cut(s) 2077
BsuRI GGCC 4 cut(s) 65, 92, 372, 727
BtgI CCRYGG 3 cut(s) 66, 87, 2021
BtgZI GCGATG 2 cut(s) 368, 1779
BtrI CACGTC 1 cut(s) 2231
BtsCI GGATG 9 cut(s) 328, 742, 768, 967, 1020, 1383, 1999, 2194, 2532
BtsI GCAGTG 1 cut(s) 2667
BtsIMutI CAGTG 4 cut(s) 647, 918, 1255, 2667
BveI ACCTGC 1 cut(s) 1349
Cac8I GCNNGC 7 cut(s) 183, 198, 285, 696, 2369, 2373, 2607
CfoI GCGC 4 cut(s) 302, 454, 2472, 2760
Cfr10I RCCGGY 3 cut(s) 181, 196, 1495
Cfr13I GGNCC 7 cut(s) 189, 370, 725, 1121, 1420, 1466, 2827
Cfr42I CCGCGG 1 cut(s) 69
Cfr9I CCCGGG 1 cut(s) 1469
CseI GACGC 2 cut(s) 187, 2180
CsiI ACCWGGT 1 cut(s) 2031
Csp6I GTAC 5 cut(s) 806, 866, 1330, 2171, 2379
CspAI ACCGGT 1 cut(s) 1495
CspCI CAANNNNNGTGG 2 cut(s) 1699, 1734
CviQI GTAC 5 cut(s) 806, 866, 1330, 2171, 2379
DdeI CTNAG 9 cut(s) 362, 927, 1231, 1287, 1416, 2154, 2520, 2770, 2860
Eam1104I CTCTTC 3 cut(s) 258, 615, 1509
EarI CTCTTC 3 cut(s) 258, 615, 1509
EciI GGCGGA 1 cut(s) 431
Ecl136II GAGCTC 2 cut(s) 2776, 2838
Eco130I CCWWGG 5 cut(s) 562, 2021, 2267, 2624, 2694
Eco24I GRGCYC 4 cut(s) 1354, 1801, 2778, 2840
Eco31I GGTCTC 1 cut(s) 839
Eco32I GATATC 1 cut(s) 1108
Eco47I GGWCC 5 cut(s) 189, 1121, 1420, 1466, 2827
Eco47III AGCGCT 1 cut(s) 301
Eco53kI GAGCTC 2 cut(s) 2776, 2838
Eco57I CTGAAG 4 cut(s) 1505, 1659, 1934, 2556
Eco88I CYCGRG 3 cut(s) 1469, 2137, 2833
EcoICRI GAGCTC 2 cut(s) 2776, 2838
EcoNI CCTNNNNNAGG 1 cut(s) 2126
EcoO109I RGGNCCY 3 cut(s) 189, 1420, 1466
EcoRI GAATTC 1 cut(s) 307
EcoRII CCWGG 5 cut(s) 997, 1117, 1714, 1793, 2031
EcoRV GATATC 1 cut(s) 1108
EcoT14I CCWWGG 5 cut(s) 562, 2021, 2267, 2624, 2694
EcoT38I GRGCYC 4 cut(s) 1354, 1801, 2778, 2840
ErhI CCWWGG 5 cut(s) 562, 2021, 2267, 2624, 2694
FalI AAGNNNNNCTT 4 cut(s) 267, 299, 546, 578
FaqI GGGAC 2 cut(s) 202, 1586
FauI CCCGC 1 cut(s) 178
FauNDI CATATG 1 cut(s) 2065
FblI GTMKAC 5 cut(s) 224, 895, 1098, 1552, 2076
FokI GGATG 9 cut(s) 335, 749, 775, 974, 1007, 1390, 2006, 2181, 2539
FriOI GRGCYC 4 cut(s) 1354, 1801, 2778, 2840
FspBI CTAG 7 cut(s) 455, 801, 869, 1869, 1977, 2057, 2123
GlaI GCGC 4 cut(s) 301, 453, 2471, 2759
GsaI CCCAGC 1 cut(s) 204
GsuI CTGGAG 1 cut(s) 1205
HaeII RGCGCY 1 cut(s) 303
HaeIII GGCC 4 cut(s) 65, 92, 372, 727
HgaI GACGC 2 cut(s) 187, 2180
HhaI GCGC 4 cut(s) 302, 454, 2472, 2760
Hin1I GRCGYC 2 cut(s) 137, 179
Hin6I GCGC 4 cut(s) 300, 452, 2470, 2758
HinP1I GCGC 4 cut(s) 300, 452, 2470, 2758
HincII GTYRAC 4 cut(s) 225, 1099, 1553, 1999
HindII GTYRAC 4 cut(s) 225, 1099, 1553, 1999
HindIII AAGCTT 2 cut(s) 20, 1476
HphI GGTGA 7 cut(s) 107, 658, 1373, 2018, 2465, 2523, 2832
Hpy99I CGWCG 8 cut(s) 169, 181, 229, 2389, 2576, 2657, 2682, 2821
HpyAV CCTTC 6 cut(s) 171, 328, 1324, 1909, 2273, 2440
HpyCH4III ACNGT 9 cut(s) 88, 124, 235, 642, 935, 1250, 1696, 1825, 2782
HpyCH4IV ACGT 7 cut(s) 137, 715, 2001, 2202, 2230, 2323, 2574
HpyF10VI GCNNNNNNNGC 6 cut(s) 71, 299, 372, 2150, 2188, 2800
HpyF3I CTNAG 9 cut(s) 362, 927, 1231, 1287, 1416, 2154, 2520, 2770, 2860
HpySE526I ACGT 7 cut(s) 137, 715, 2001, 2202, 2230, 2323, 2574
Hsp92I GRCGYC 2 cut(s) 137, 179
HspAI GCGC 4 cut(s) 300, 452, 2470, 2758
Kpn2I TCCGGA 1 cut(s) 328
KroI GCCGGC 2 cut(s) 181, 196
KroNI GCCGGC 2 cut(s) 183, 198
KspI CCGCGG 1 cut(s) 69
LguI GCTCTTC 1 cut(s) 1509
LmnI GCTCC 3 cut(s) 154, 1804, 2602
Lsp1109I GCAGC 4 cut(s) 345, 1161, 2131, 2812
LweI GCATC 7 cut(s) 345, 727, 876, 1571, 1635, 1756, 1819
MabI ACCWGGT 1 cut(s) 2031
MaeI CTAG 7 cut(s) 455, 801, 869, 1869, 1977, 2057, 2123
MaeII ACGT 7 cut(s) 137, 715, 2001, 2202, 2230, 2323, 2574
MaeIII GTNAC 6 cut(s) 664, 1244, 1658, 1931, 2224, 2529
MflI RGATCY 5 cut(s) 342, 818, 982, 1918, 2688
MhlI GDGCHC 4 cut(s) 1354, 1801, 2778, 2840
MlyI GAGTC 4 cut(s) 166, 230, 395, 2598
MmeI TCCRAC 3 cut(s) 240, 936, 1247
MreI CGCCGGCG 1 cut(s) 181
MroI TCCGGA 1 cut(s) 328
MroNI GCCGGC 2 cut(s) 181, 196
MroXI GAANNNNTTC 3 cut(s) 1218, 1518, 2117
MseI TTAA 5 cut(s) 903, 942, 1047, 1446, 1779
MslI CAYNNNNRTG 3 cut(s) 1092, 1359, 1570
MspA1I CMGCKG 2 cut(s) 68, 2803
MspCI CTTAAG 1 cut(s) 902
MspR9I CCNGG 9 cut(s) 347, 999, 1119, 1470, 1471, 1716, 1795, 2033, 2399
MvaI CCWGG 5 cut(s) 999, 1119, 1716, 1795, 2033
MvnI CGCG 3 cut(s) 68, 430, 2470
MwoI GCNNNNNNNGC 6 cut(s) 71, 299, 372, 2150, 2188, 2800
NaeI GCCGGC 2 cut(s) 183, 198
NciI CCSGG 4 cut(s) 347, 1470, 1471, 2399
NcoI CCATGG 1 cut(s) 2021
NdeI CATATG 1 cut(s) 2065
NgoMIV GCCGGC 2 cut(s) 181, 196
NlaIV GGNNCC 8 cut(s) 109, 190, 820, 1422, 1468, 1800, 1858, 1920
NmuCI GTSAC 3 cut(s) 664, 1244, 2529
NspI RCATGY 1 cut(s) 2375
NspV TTCGAA 2 cut(s) 305, 1317
PaeI GCATGC 1 cut(s) 2375
PaqCI CACCTGC 1 cut(s) 1349
PasI CCCWGGG 1 cut(s) 1794
PciSI GCTCTTC 1 cut(s) 1509
PcsI WCGNNNNNNNCGW 1 cut(s) 173
PdiI GCCGGC 2 cut(s) 183, 198
PdmI GAANNNNTTC 3 cut(s) 1218, 1518, 2117
PfeI GAWTC 7 cut(s) 512, 1214, 1342, 1898, 1946, 2674, 2821
PflFI GACNNNGTC 1 cut(s) 424
PflMI CCANNNNNTGG 1 cut(s) 1136
PfoI TCCNGGA 1 cut(s) 997
PinAI ACCGGT 1 cut(s) 1495
Ple19I CGATCG 1 cut(s) 56
PleI GAGTC 4 cut(s) 165, 229, 394, 2597
PpsI GAGTC 4 cut(s) 165, 229, 394, 2597
PpuMI RGGWCCY 3 cut(s) 189, 1420, 1466
PshAI GACNNNNGTC 1 cut(s) 2390
Psp124BI GAGCTC 2 cut(s) 2778, 2840
Psp1406I AACGTT 1 cut(s) 2202
Psp5II RGGWCCY 3 cut(s) 189, 1420, 1466
Psp6I CCWGG 5 cut(s) 997, 1117, 1714, 1793, 2031
PspFI CCCAGC 1 cut(s) 200
PspGI CCWGG 5 cut(s) 997, 1117, 1714, 1793, 2031
PspN4I GGNNCC 8 cut(s) 109, 190, 820, 1422, 1468, 1800, 1858, 1920
PspPI GGNCC 7 cut(s) 189, 370, 725, 1121, 1420, 1466, 2827
PspPPI RGGWCCY 3 cut(s) 189, 1420, 1466
PsrI GAACNNNNNNTAC 2 cut(s) 1377, 1409
PsuI RGATCY 5 cut(s) 342, 818, 982, 1918, 2688
PsyI GACNNNGTC 1 cut(s) 424
PvuI CGATCG 1 cut(s) 56
RsaI GTAC 5 cut(s) 807, 867, 1331, 2172, 2380
RsaNI GTAC 5 cut(s) 806, 866, 1330, 2171, 2379
RseI CAYNNNNRTG 3 cut(s) 1092, 1359, 1570
SacI GAGCTC 2 cut(s) 2778, 2840
SacII CCGCGG 1 cut(s) 69
SalI GTCGAC 3 cut(s) 223, 1097, 1551
SapI GCTCTTC 1 cut(s) 1509
SaqAI TTAA 5 cut(s) 903, 942, 1047, 1446, 1779
Sau96I GGNCC 7 cut(s) 189, 370, 725, 1121, 1420, 1466, 2827
SchI GAGTC 4 cut(s) 166, 230, 395, 2598
ScrFI CCNGG 9 cut(s) 347, 999, 1119, 1470, 1471, 1716, 1795, 2033, 2399
SduI GDGCHC 4 cut(s) 1354, 1801, 2778, 2840
SexAI ACCWGGT 1 cut(s) 2031
SfaNI GCATC 7 cut(s) 345, 727, 876, 1571, 1635, 1756, 1819
SfcI CTRYAG 1 cut(s) 892
Sfr303I CCGCGG 1 cut(s) 69
SfuI TTCGAA 2 cut(s) 305, 1317
SgrAI CRCCGGYG 1 cut(s) 181
SgrBI CCGCGG 1 cut(s) 69
SinI GGWCC 5 cut(s) 189, 1121, 1420, 1466, 2827
SmaI CCCGGG 1 cut(s) 1471
SmiMI CAYNNNNRTG 3 cut(s) 1092, 1359, 1570
SmlI CTYRAG 3 cut(s) 902, 2684, 2789
SmoI CTYRAG 3 cut(s) 902, 2684, 2789
SpeI ACTAGT 1 cut(s) 800
SphI GCATGC 1 cut(s) 2375
SspMI CTAG 7 cut(s) 455, 801, 869, 1869, 1977, 2057, 2123
SstI GAGCTC 2 cut(s) 2778, 2840
StyD4I CCNGG 9 cut(s) 345, 997, 1117, 1468, 1469, 1714, 1793, 2031, 2397
StyI CCWWGG 5 cut(s) 562, 2021, 2267, 2624, 2694
TaaI ACNGT 9 cut(s) 88, 124, 235, 642, 935, 1250, 1696, 1825, 2782
TaiI ACGT 7 cut(s) 140, 718, 2004, 2205, 2233, 2326, 2577
TaqII GACCGA 1 cut(s) 2759
TatI WGTACW 3 cut(s) 805, 1329, 2378
TauI GCSGC 9 cut(s) 68, 71, 74, 353, 375, 413, 416, 433, 2806
TfiI GAWTC 7 cut(s) 512, 1214, 1342, 1898, 1946, 2674, 2821
Tru1I TTAA 5 cut(s) 903, 942, 1047, 1446, 1779
Tru9I TTAA 5 cut(s) 903, 942, 1047, 1446, 1779
TscAI CASTG 4 cut(s) 647, 925, 1255, 2667
TseFI GTSAC 3 cut(s) 664, 1244, 2529
TseI GCWGC 4 cut(s) 358, 1149, 2144, 2800
Tsp45I GTSAC 3 cut(s) 664, 1244, 2529
TspGWI ACGGA 2 cut(s) 2114, 2805
TspMI CCCGGG 1 cut(s) 1469
TspRI CASTG 4 cut(s) 647, 925, 1255, 2667
Tth111I GACNNNGTC 1 cut(s) 424
Van91I CCANNNNNTGG 1 cut(s) 1136
Vha464I CTTAAG 1 cut(s) 902
VpaK11BI GGWCC 5 cut(s) 189, 1121, 1420, 1466, 2827
XagI CCTNNNNNAGG 1 cut(s) 2126
XapI RAATTY 3 cut(s) 307, 1321, 2016
XceI RCATGY 1 cut(s) 2375
XmaI CCCGGG 1 cut(s) 1469
XmiI GTMKAC 5 cut(s) 224, 895, 1098, 1552, 2076
XmnI GAANNNNTTC 3 cut(s) 1218, 1518, 2117
XspI CTAG 7 cut(s) 455, 801, 869, 1869, 1977, 2057, 2123
ZraI GACGTC 1 cut(s) 138
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.