MD12G1234900.v1.1

Serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0004456
Physical Location & Seq
Reverse (-)
30972476 .. 30987983
15508 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1234900.v1.1.491

Sequence Viewer

Length: 2916 bp
ATGAAGCACATTTTCAAGAAGCTTCACATAGGGAGCAACCACGACCCGACCCGATCGAACGAGGCCGCCGCCGCCGCGGCCTCCTCCTCCTCCGTAGCCTCGCCGTCGTGCGCCTCCGATCACCGGACCGCGTCGGGTCAGATTTCCGGAAACGCTCCGGCGAGTCCTTCATCGTCTTCGCCGACGCCGGCGGGAACCGTGGCAGCTGGGGGAGGTGGCACTGTGGAGTCGACGACTGTGAATCGGTCGGACTTTATGATGTCGGAAGAGGAGTATCAGGTCCAGCTCGCCCTGGCGATCAGCGCTTCGAATTCGAACTTTAGGGATGATCCGGAGAAGGATCAGATCCGCGCGGCGACGCTGCTGAGCTTGGGCGGCAATCCGATCGATTCTTCAAGAGATAAGGTTGAGGCGGCGGCGGAGACCTTGTCGCGGCACTATTGGGAGTACAATGTGCTAGACTATGAGGAGAAAGTGGTTGATGGATTTTATGATGTATATGGACTTTCCACAGATTCAGCAATTCAAGGAAAAATGCCTTCTCTCACAAATCTTGAAACAAACTTTGGGAGTTCTGGATTTGAAGTTTCATTAGTAAATCGAACAGTTGATCCTGTCTTGGAAGAGTTGGTGCAAATTGCACAGTGTATTGCTTTAGACTGTCCTGTCACCAACGTTGGTGTTTTGGTTCTGAGGCTTGCTGAACTCGTTAGCGAACGTATGGGTGGGCCTGTAAAGGATGCAAATGTTGTCTTAGCGAGATGGATGGAAAGAAGCACAGAGTTGAGGACATCTCTGCAAACAAGTGTATTGCCTATTGGGTCCATAACCATTGGCCTTTCCCGACATCGTGCTTTGCTTTTCAAGGTACTAGCTGACAATATCAAGATGCCCTGCAGACTGCTTAAGGGTGTTCATTACACTGGTGCTGAGGATGGTGCTGTTAATGTAATAAAGTTGGAGGATGACAGGGAGTTTTTGGTGGATCTGATGGCTGATCCTGGAACACTCGTACCAACTGACACCCAAATTTCAAAGGACGCTACCTTTAAGCCATACAATCCAAACTTGACCAAACTTCCTACTTTCCATTCTTATATCGACACAGATATCGCTTACCCAGGACCAAAACCATTACATGGAGAGGGAAGCAGCCAAAACACAGCAGTTGAAAGTAGTTCAGGTCTGGAGAGAAGACCAAGCTCAGATAAGGGAGAATCATTTCCAACATTTTCGGGTGCAAGTAGTGAGAGTGGAGTTGGTTCTTCAGGAATACCAAATAAAGCAACTCAGTTAGATCAGCTTTCCTCATCAGCCTCCAAAAAATCACAGTACAACAGAGGTAATCGTGGGGCTCATGCAATTGATGGTGGTATGAGGATGAATGTAAATATAGTTCCATGTACTCAAAACAACTCTGAGGACCCAAAGAACCTTTTTGCTGATCTTAACCCATTCCAAATAAAAGGACCTGGGAAAGCTTCTATGTATAACAAACCTGCGGAGAAAAAAGTTGAAGAGCTTCAGAGACAAAATAATAATCTTGCTTCTGGTCGACCTCCTGCATCTTTGATGTGGAAAAATAAATATGCTTTCAATGAAGTCCCTAAAAGAAAGGAGTATGATGGTATCCTCCCAAAAATTAATCGTGAGCCTAATGGTTACAATCCGTCATCTTCAGCTTCGACCAGCTCTACTGTATCTGAACAGGTCAATCCTGGTGGTTTCAAATCAACCGCTCATTCAAATATGTTTGACAGAGATGGTGATGCCAAAATTTTTAAAGGGGAACAACCCAGGGCTCCGAACTATTTACAGAACAACACAGTAGATTCTAAAGAACATCAGAACATTGAAACTGGTTTCAATGATCCTAGAAAGTTCACACATGACAGATTTATGGAATCCAATTTGAAACTGAAGGATCCAGAAAGTTGTAACTCATCATTTGATTCCATTACAAGCAGGGTTGAATATCATTCTAGCACTAGCTCATCATTTGAGGATGTTGACGTAGGTGAATGTGAAATTCCATGGGAAGACCTGGTTATTGGAGAAAGGATTGGACTAGGTTCATATGGTGAGGTATACCATGCTGATTGGCATGGCACGGAAGTTGCCGTGAAGAAGTTCTTAGACCAGGATTTCTCGGGTGCTGCTTTGGCTGAGTTCAAAAGAGAAGTACGAATAATGCGTAGATTGCGTCATCCAAACGTTGTGCTTTTCATGGGGGCTGTTACACGTCCTCCAAACCTCTCTATCATTTCTGAGTTTCTTCCGAGGGGAAGTCTATATCGGATTATTCATCGTCCTCATTGTCAAATTGACGAGAAACGTAGAATAAAGATGGCTCTGGATGTGGCAAGGGGTATGAATTGCTTGCATGCCAGTACACCGACTATTGTTCACCGGGATTTGAAGTCTCCGAATCTGCTGGTTGATAAGAACTGGAATGTGAAGGTGTGCGATTTTGGTTTGTCACGCTTGAAACACAACACTTTCTTGTCGTCCAAATCAACTGCTGGAACGCCTGAGTGGATGGCACCTGAAGTTCTCCGCAATGAAAACTCAAATGAAAAGTGTGACGTATATAGCTTTGGAGTCATTTTGTGGGAGCTTGCAACTCTAAAACTGCCGTGGAGTGGGATGAACCCAATGCAGGTTGTCGGTGCTGTGGGTTTCCAGAACCGTCGCCTTGAGATCCCCAAGGAACTTGATCCCCTTGTTGCAAGGATAATTTGGGAATGTTGGCAGACCGATCCGAACTTGCGCCCCTCGTTCTCACAGCTCACGGTAGCTCTCAAGCCCTTGCAGCGACTTGTCATTCCGTCGAATCTGGACGACCCGAGCTCACATCTCCGGCAAGAAATCTCAGTAAATTCTACGCCTTTAAACGCCTCATTCTCTCAACTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000165 GO:0000186 GO:0001101 GO:0001932 GO:0001934 GO:0002229 GO:0002239 GO:0002831 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0004709 GO:0004712 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005768 GO:0005769 GO:0005783 GO:0005794 GO:0005798 GO:0005802 GO:0005829 GO:0005886 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0006952 GO:0007154 GO:0007165 GO:0008150 GO:0008152 GO:0008219 GO:0009414 GO:0009415 GO:0009605 GO:0009607 GO:0009617 GO:0009620 GO:0009628 GO:0009719 GO:0009723 GO:0009725 GO:0009787 GO:0009788 GO:0009893 GO:0009966 GO:0009967 GO:0009968 GO:0009987 GO:0010033 GO:0010035 GO:0010562 GO:0010604 GO:0010646 GO:0010647 GO:0010648 GO:0012505 GO:0012506 GO:0012510 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019220 GO:0019222 GO:0019538 GO:0023014 GO:0023051 GO:0023052 GO:0023056 GO:0023057 GO:0030133 GO:0030135 GO:0030136 GO:0030140 GO:0030658 GO:0030659 GO:0030660 GO:0030662 GO:0030665 GO:0031090 GO:0031323 GO:0031325 GO:0031347 GO:0031399 GO:0031401 GO:0031410 GO:0031982 GO:0031984 GO:0032101 GO:0032147 GO:0032268 GO:0032270 GO:0033674 GO:0035556 GO:0036211 GO:0042221 GO:0042325 GO:0042327 GO:0043085 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043405 GO:0043406 GO:0043408 GO:0043410 GO:0043412 GO:0043549 GO:0043900 GO:0044093 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044431 GO:0044433 GO:0044444 GO:0044446 GO:0044464 GO:0045859 GO:0045860 GO:0045937 GO:0046777 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048583 GO:0048584 GO:0048585 GO:0050789 GO:0050790 GO:0050794 GO:0050896 GO:0051171 GO:0051173 GO:0051174 GO:0051246 GO:0051247 GO:0051338 GO:0051347 GO:0051704 GO:0051707 GO:0051716 GO:0060255 GO:0065007 GO:0065009 GO:0071704 GO:0071900 GO:0071902 GO:0071944 GO:0080090 GO:0080134 GO:0097708 GO:0098542 GO:0098588 GO:0098791 GO:0098805 GO:0140096 GO:1900150 GO:1900424 GO:1901419 GO:1901420 GO:1901564 GO:1901700 GO:1902531 GO:1902533 GO:1905957 GO:1905958 GO:2000031 GO:2001023 GO:2001038
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EDR1 Pkinase_Tyr

Protein Analysis

972

Amino Acids

106.74

Weight (kDa)

6.14

Isoelectric Point (pI)

44.31

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EDR1_CTR1_ARMC3_pept PF14381 140 - 338 1.4e-73 EDR1/CTR1/ARMC3, peptidase-like
PK_Tyr_Ser-Thr PF07714 683 - 932 1.3e-62 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 684 - 930 6.5e-51 Protein kinase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 665
Acc36I ACCTGC 2 cut(s) 1507, 2650
AccB1I GGYRCC 1 cut(s) 2542
AccB7I CCANNNNNTGG 1 cut(s) 1139
AccBSI CCGCTC 1 cut(s) 1739
AccI GTMKAC 3 cut(s) 230, 1555, 2088
AccII CGCG 5 cut(s) 77, 131, 351, 353, 433
AccIII TCCGGA 2 cut(s) 146, 331
AclI AACGTT 2 cut(s) 675, 2214
AcsI RAATTY 5 cut(s) 310, 1029, 1776, 2028, 2878
AcuI CTGAAG 5 cut(s) 1251, 1508, 1662, 1940, 2568
AcyI GRCGYC 1 cut(s) 185
AfaI GTAC 7 cut(s) 449, 870, 1014, 1334, 1405, 2184, 2392
AfeI AGCGCT 1 cut(s) 304
AfiI CCNNNNNNNGG 5 cut(s) 123, 432, 1139, 2642, 2659
AflII CTTAAG 1 cut(s) 905
AflIII ACRYGT 1 cut(s) 2240
AjiI CACGTC 1 cut(s) 2243
AjnI CCWGG 8 cut(s) 291, 1000, 1120, 1471, 1717, 1796, 2043, 2139
AjuI GAANNNNNNNTTGG 2 cut(s) 549, 581
AloI GAACNNNNNNTCC 2 cut(s) 595, 627
Alw21I GWGCWC 1 cut(s) 2852
Alw26I GTCTC 3 cut(s) 416, 1522, 2427
Ama87I CYCGRG 2 cut(s) 2149, 2845
Aor13HI TCCGGA 2 cut(s) 146, 331
Aor51HI AGCGCT 1 cut(s) 304
AoxI GGCC 4 cut(s) 63, 78, 728, 835
ApeKI GCWGC 5 cut(s) 203, 361, 1152, 2156, 2812
ApoI RAATTY 5 cut(s) 310, 1029, 1776, 2028, 2878
AseI ATTAAT 1 cut(s) 1644
Asp700I GAANNNNTTC 3 cut(s) 1221, 1521, 2129
AspLEI GCGC 4 cut(s) 113, 305, 353, 2772
AspS9I GGNCC 7 cut(s) 126, 280, 728, 822, 1124, 1423, 1469
AsuC2I CCSGG 1 cut(s) 2411
AsuHPI GGTGA 6 cut(s) 113, 661, 1778, 2030, 2093, 2399
AsuII TTCGAA 2 cut(s) 308, 314
AvaI CYCGRG 2 cut(s) 2149, 2845
AvaII GGWCC 6 cut(s) 126, 280, 822, 1124, 1423, 1469
BaeI ACNNNNGTAYC 2 cut(s) 996, 1029
BamHI GGATCC 1 cut(s) 1924
BanI GGYRCC 1 cut(s) 2542
BanII GRGCYC 3 cut(s) 1357, 1804, 2852
BbsI GAAGAC 3 cut(s) 168, 1201, 2046
Bbv12I GWGCWC 1 cut(s) 2852
BbvCI CCTCAGC 1 cut(s) 930
BbvI GCAGC 5 cut(s) 215, 348, 1164, 2143, 2824
BceAI ACGGC 3 cut(s) 88, 2105, 2620
BcgI CGANNNNNNTGC 2 cut(s) 367, 401
BciT130I CCWGG 8 cut(s) 293, 1002, 1122, 1473, 1719, 1798, 2045, 2141
BciVI GTATCC 1 cut(s) 1640
BcnI CCSGG 1 cut(s) 2411
BcoDI GTCTC 3 cut(s) 416, 1522, 2427
BfaI CTAG 6 cut(s) 458, 872, 1875, 1983, 1989, 2069
BfmI CTRYAG 1 cut(s) 895
BfoI RGCGCY 1 cut(s) 306
BfrI CTTAAG 1 cut(s) 905
BfuAI ACCTGC 2 cut(s) 1507, 2650
BfuI GTATCC 1 cut(s) 1640
BglI GCCNNNNNGGC 1 cut(s) 77
BlpI GCTNAGC 1 cut(s) 365
Bme1390I CCNGG 9 cut(s) 293, 1002, 1122, 1473, 1719, 1798, 2045, 2141, 2411
Bme18I GGWCC 6 cut(s) 126, 280, 822, 1124, 1423, 1469
BmeT110I CYCGRG 2 cut(s) 2149, 2845
BmgBI CACGTC 1 cut(s) 2243
BmgT120I GGNCC 7 cut(s) 126, 280, 728, 822, 1124, 1423, 1469
BmiI GGNNCC 6 cut(s) 196, 823, 1425, 1803, 1926, 2544
BmrFI CCNGG 9 cut(s) 293, 1002, 1122, 1473, 1719, 1798, 2045, 2141, 2411
BmsI GCATC 4 cut(s) 730, 879, 1574, 1759
BpiI GAAGAC 3 cut(s) 168, 1201, 2046
BplI GAGNNNNNCTC 2 cut(s) 778, 810
BpmI CTGGAG 1 cut(s) 1208
Bpu10I CCTNAGC 1 cut(s) 930
Bpu1102I GCTNAGC 1 cut(s) 365
Bpu14I TTCGAA 2 cut(s) 308, 314
BpuEI CTTGAG 2 cut(s) 2717, 2786
BpuMI CCSGG 1 cut(s) 2411
Bsa29I ATCGAT 1 cut(s) 387
BsaHI GRCGYC 1 cut(s) 185
BsaI GGTCTC 1 cut(s) 416
BsaWI WCCGGW 3 cut(s) 123, 146, 331
BsaXI ACNNNNNCTCC 4 cut(s) 413, 443, 2230, 2260
Bsc4I CCNNNNNNNGG 5 cut(s) 123, 432, 1139, 2642, 2659
Bse118I RCCGGY 1 cut(s) 187
Bse1I ACTGG 4 cut(s) 928, 1864, 2388, 2453
Bse3DI GCAATG 1 cut(s) 2566
BseAI TCCGGA 2 cut(s) 146, 331
BseBI CCWGG 8 cut(s) 293, 1002, 1122, 1473, 1719, 1798, 2045, 2141
BseCI ATCGAT 1 cut(s) 387
BseLI CCNNNNNNNGG 5 cut(s) 123, 432, 1139, 2642, 2659
BseMI GCAATG 1 cut(s) 2566
BseNI ACTGG 4 cut(s) 928, 1864, 2388, 2453
BseRI GAGGAG 5 cut(s) 73, 76, 79, 284, 482
BseXI GCAGC 5 cut(s) 215, 348, 1164, 2143, 2824
BseYI CCCAGC 1 cut(s) 206
Bsh1236I CGCG 5 cut(s) 77, 131, 351, 353, 433
Bsh1285I CGRYCG 3 cut(s) 56, 248, 387
BshFI GGCC 4 cut(s) 65, 80, 730, 837
BshNI GGYRCC 1 cut(s) 2542
BshVI ATCGAT 1 cut(s) 387
BsiEI CGRYCG 3 cut(s) 56, 248, 387
BsiHKAI GWGCWC 1 cut(s) 2852
BsiHKCI CYCGRG 2 cut(s) 2149, 2845
BsiSI CCGG 7 cut(s) 124, 147, 158, 188, 332, 2410, 2860
BslFI GGGAC 1 cut(s) 1589
BslI CCNNNNNNNGG 5 cut(s) 123, 432, 1139, 2642, 2659
BsmAI GTCTC 3 cut(s) 416, 1522, 2427
BsmFI GGGAC 1 cut(s) 1589
BsnI GGCC 4 cut(s) 65, 80, 730, 837
Bso31I GGTCTC 1 cut(s) 416
BsoBI CYCGRG 2 cut(s) 2149, 2845
Bsp119I TTCGAA 2 cut(s) 308, 314
Bsp1286I GDGCHC 3 cut(s) 1357, 1804, 2852
Bsp13I TCCGGA 2 cut(s) 146, 331
Bsp1720I GCTNAGC 1 cut(s) 365
Bsp19I CCATGG 1 cut(s) 2033
BspANI GGCC 4 cut(s) 65, 80, 730, 837
BspDI ATCGAT 1 cut(s) 387
BspEI TCCGGA 2 cut(s) 146, 331
BspFNI CGCG 5 cut(s) 77, 131, 351, 353, 433
BspLI GGNNCC 6 cut(s) 196, 823, 1425, 1803, 1926, 2544
BspMAI CTGCAG 1 cut(s) 899
BspMI ACCTGC 2 cut(s) 1507, 2650
BspQI GCTCTTC 1 cut(s) 1512
BspT104I TTCGAA 2 cut(s) 308, 314
BspT107I GGYRCC 1 cut(s) 2542
BspTI CTTAAG 1 cut(s) 905
BspTNI GGTCTC 1 cut(s) 416
BsrBI CCGCTC 1 cut(s) 1739
BsrDI GCAATG 1 cut(s) 2566
BsrFI RCCGGY 1 cut(s) 187
BsrI ACTGG 4 cut(s) 928, 1864, 2388, 2453
BssAI RCCGGY 1 cut(s) 187
BssNAI GTATAC 1 cut(s) 2089
BssNI GRCGYC 1 cut(s) 185
BssT1I CCWWGG 2 cut(s) 2033, 2706
Bst1107I GTATAC 1 cut(s) 2089
Bst2UI CCWGG 8 cut(s) 293, 1002, 1122, 1473, 1719, 1798, 2045, 2141
Bst6I CTCTTC 3 cut(s) 261, 618, 1512
BstACI GRCGYC 1 cut(s) 185
BstAFI CTTAAG 1 cut(s) 905
BstBI TTCGAA 2 cut(s) 308, 314
BstC8I GCNNGC 6 cut(s) 189, 288, 699, 2381, 2385, 2619
BstDSI CCRYGG 4 cut(s) 75, 198, 2033, 2636
BstFNI CGCG 5 cut(s) 77, 131, 351, 353, 433
BstH2I RGCGCY 1 cut(s) 306
BstHHI GCGC 4 cut(s) 113, 305, 353, 2772
BstMAI GTCTC 3 cut(s) 416, 1522, 2427
BstMCI CGRYCG 3 cut(s) 56, 248, 387
BstMWI GCNNNNNNNGC 8 cut(s) 71, 74, 77, 302, 375, 2162, 2200, 2812
BstNI CCWGG 8 cut(s) 293, 1002, 1122, 1473, 1719, 1798, 2045, 2141
BstNSI RCATGY 1 cut(s) 2387
BstSCI CCNGG 9 cut(s) 291, 1000, 1120, 1471, 1717, 1796, 2043, 2139, 2409
BstSFI CTRYAG 1 cut(s) 895
BstUI CGCG 5 cut(s) 77, 131, 351, 353, 433
BstV1I GCAGC 5 cut(s) 215, 348, 1164, 2143, 2824
BstV2I GAAGAC 3 cut(s) 168, 1201, 2046
BstX2I RGATCY 4 cut(s) 345, 985, 1924, 2700
BstYI RGATCY 4 cut(s) 345, 985, 1924, 2700
BstZ17I GTATAC 1 cut(s) 2089
Bsu15I ATCGAT 1 cut(s) 387
BsuI GTATCC 1 cut(s) 1640
BsuRI GGCC 4 cut(s) 65, 80, 730, 837
BsuTUI ATCGAT 1 cut(s) 387
BtgI CCRYGG 4 cut(s) 75, 198, 2033, 2636
BtrI CACGTC 1 cut(s) 2243
BtsIMutI CAGTG 3 cut(s) 219, 650, 921
BveI ACCTGC 2 cut(s) 1507, 2650
Cac8I GCNNGC 6 cut(s) 189, 288, 699, 2381, 2385, 2619
CfoI GCGC 4 cut(s) 113, 305, 353, 2772
Cfr10I RCCGGY 1 cut(s) 187
Cfr13I GGNCC 7 cut(s) 126, 280, 728, 822, 1124, 1423, 1469
Cfr42I CCGCGG 1 cut(s) 78
ClaI ATCGAT 1 cut(s) 387
CpoI CGGWCCG 1 cut(s) 126
CseI GACGC 5 cut(s) 120, 193, 367, 1049, 2192
CsiI ACCWGGT 1 cut(s) 2043
Csp6I GTAC 7 cut(s) 448, 869, 1013, 1333, 1404, 2183, 2391
CspCI CAANNNNNGTGG 2 cut(s) 1702, 1737
CspI CGGWCCG 1 cut(s) 126
CviAII CATG 9 cut(s) 1139, 1358, 1401, 1889, 2034, 2093, 2105, 2227, 2384
CviQI GTAC 7 cut(s) 448, 869, 1013, 1333, 1404, 2183, 2391
DraI TTTAAA 2 cut(s) 1783, 2892
DrdI GACNNNNNNGTC 1 cut(s) 665
DseDI GACNNNNNNGTC 1 cut(s) 665
Eam1104I CTCTTC 3 cut(s) 261, 618, 1512
EarI CTCTTC 3 cut(s) 261, 618, 1512
EciI GGCGGA 1 cut(s) 434
Ecl136II GAGCTC 1 cut(s) 2850
Eco130I CCWWGG 2 cut(s) 2033, 2706
Eco24I GRGCYC 3 cut(s) 1357, 1804, 2852
Eco31I GGTCTC 1 cut(s) 416
Eco32I GATATC 1 cut(s) 1111
Eco47I GGWCC 6 cut(s) 126, 280, 822, 1124, 1423, 1469
Eco47III AGCGCT 1 cut(s) 304
Eco53kI GAGCTC 1 cut(s) 2850
Eco57I CTGAAG 5 cut(s) 1251, 1508, 1662, 1940, 2568
Eco88I CYCGRG 2 cut(s) 2149, 2845
EcoICRI GAGCTC 1 cut(s) 2850
EcoO109I RGGNCCY 2 cut(s) 1423, 1469
EcoRI GAATTC 1 cut(s) 310
EcoRII CCWGG 8 cut(s) 291, 1000, 1120, 1471, 1717, 1796, 2043, 2139
EcoRV GATATC 1 cut(s) 1111
EcoT14I CCWWGG 2 cut(s) 2033, 2706
EcoT38I GRGCYC 3 cut(s) 1357, 1804, 2852
ErhI CCWWGG 2 cut(s) 2033, 2706
FaeI CATG 9 cut(s) 1142, 1361, 1404, 1892, 2037, 2096, 2108, 2230, 2387
FalI AAGNNNNNCTT 2 cut(s) 2117, 2149
FaqI GGGAC 1 cut(s) 1589
FatI CATG 9 cut(s) 1138, 1357, 1400, 1888, 2033, 2092, 2104, 2226, 2383
FauI CCCGC 1 cut(s) 184
FauNDI CATATG 1 cut(s) 2077
FblI GTMKAC 3 cut(s) 230, 1555, 2088
FriOI GRGCYC 3 cut(s) 1357, 1804, 2852
FspBI CTAG 6 cut(s) 458, 872, 1875, 1983, 1989, 2069
GlaI GCGC 4 cut(s) 112, 304, 352, 2771
GsaI CCCAGC 1 cut(s) 210
GsuI CTGGAG 1 cut(s) 1208
HaeII RGCGCY 1 cut(s) 306
HaeIII GGCC 4 cut(s) 65, 80, 730, 837
HapII CCGG 7 cut(s) 124, 147, 158, 188, 332, 2410, 2860
HgaI GACGC 5 cut(s) 120, 193, 367, 1049, 2192
HhaI GCGC 4 cut(s) 113, 305, 353, 2772
Hin1I GRCGYC 1 cut(s) 185
Hin1II CATG 9 cut(s) 1142, 1361, 1404, 1892, 2037, 2096, 2108, 2230, 2387
Hin6I GCGC 4 cut(s) 111, 303, 351, 2770
HinP1I GCGC 4 cut(s) 111, 303, 351, 2770
HincII GTYRAC 3 cut(s) 231, 1556, 2011
HindII GTYRAC 3 cut(s) 231, 1556, 2011
HindIII AAGCTT 2 cut(s) 20, 1479
HpaII CCGG 7 cut(s) 124, 147, 158, 188, 332, 2410, 2860
HphI GGTGA 6 cut(s) 113, 661, 1778, 2030, 2093, 2399
Hpy166II GTNNAC 7 cut(s) 231, 1556, 1884, 2011, 2089, 2393, 2407
Hpy8I GTNNAC 7 cut(s) 231, 1556, 1884, 2011, 2089, 2393, 2407
Hpy99I CGWCG 7 cut(s) 109, 136, 187, 235, 361, 2694, 2833
HpyAV CCTTC 5 cut(s) 177, 331, 549, 1915, 2452
HpyCH4IV ACGT 7 cut(s) 675, 718, 2013, 2214, 2242, 2335, 2586
HpyF10VI GCNNNNNNNGC 8 cut(s) 71, 74, 77, 302, 375, 2162, 2200, 2812
HpySE526I ACGT 7 cut(s) 675, 718, 2013, 2214, 2242, 2335, 2586
Hsp92I GRCGYC 1 cut(s) 185
Hsp92II CATG 9 cut(s) 1142, 1361, 1404, 1892, 2037, 2096, 2108, 2230, 2387
HspAI GCGC 4 cut(s) 111, 303, 351, 2770
Kpn2I TCCGGA 2 cut(s) 146, 331
KroI GCCGGC 1 cut(s) 187
KroNI GCCGGC 1 cut(s) 189
KspI CCGCGG 1 cut(s) 78
LguI GCTCTTC 1 cut(s) 1512
LmnI GCTCC 4 cut(s) 33, 160, 1807, 2614
Lsp1109I GCAGC 5 cut(s) 215, 348, 1164, 2143, 2824
LweI GCATC 4 cut(s) 730, 879, 1574, 1759
MabI ACCWGGT 1 cut(s) 2043
MaeI CTAG 6 cut(s) 458, 872, 1875, 1983, 1989, 2069
MaeII ACGT 7 cut(s) 675, 718, 2013, 2214, 2242, 2335, 2586
MaeIII GTNAC 6 cut(s) 667, 1661, 1937, 2236, 2478, 2582
MbiI CCGCTC 1 cut(s) 1739
MfeI CAATTG 1 cut(s) 1362
MflI RGATCY 4 cut(s) 345, 985, 1924, 2700
MhlI GDGCHC 3 cut(s) 1357, 1804, 2852
MlyI GAGTC 3 cut(s) 172, 236, 2610
MmeI TCCRAC 4 cut(s) 228, 243, 939, 1250
MreI CGCCGGCG 1 cut(s) 187
MroI TCCGGA 2 cut(s) 146, 331
MroNI GCCGGC 1 cut(s) 187
MroXI GAANNNNTTC 3 cut(s) 1221, 1521, 2129
MseI TTAA 7 cut(s) 906, 945, 1050, 1449, 1644, 1782, 2891
MspA1I CMGCKG 2 cut(s) 77, 206
MspCI CTTAAG 1 cut(s) 905
MspI CCGG 7 cut(s) 124, 147, 158, 188, 332, 2410, 2860
MspR9I CCNGG 9 cut(s) 293, 1002, 1122, 1473, 1719, 1798, 2045, 2141, 2411
MunI CAATTG 1 cut(s) 1362
MvaI CCWGG 8 cut(s) 293, 1002, 1122, 1473, 1719, 1798, 2045, 2141
MvnI CGCG 5 cut(s) 77, 131, 351, 353, 433
MwoI GCNNNNNNNGC 8 cut(s) 71, 74, 77, 302, 375, 2162, 2200, 2812
NaeI GCCGGC 1 cut(s) 189
NciI CCSGG 1 cut(s) 2411
NcoI CCATGG 1 cut(s) 2033
NdeI CATATG 1 cut(s) 2077
NgoMIV GCCGGC 1 cut(s) 187
NlaIII CATG 9 cut(s) 1142, 1361, 1404, 1892, 2037, 2096, 2108, 2230, 2387
NlaIV GGNNCC 6 cut(s) 196, 823, 1425, 1803, 1926, 2544
NmuCI GTSAC 3 cut(s) 667, 2478, 2582
NspI RCATGY 1 cut(s) 2387
NspV TTCGAA 2 cut(s) 308, 314
PaeI GCATGC 1 cut(s) 2387
PasI CCCWGGG 1 cut(s) 1797
PciSI GCTCTTC 1 cut(s) 1512
PcsI WCGNNNNNNNCGW 2 cut(s) 179, 2191
PdiI GCCGGC 1 cut(s) 189
PdmI GAANNNNTTC 3 cut(s) 1221, 1521, 2129
PfeI GAWTC 9 cut(s) 241, 389, 515, 1217, 1832, 1904, 1952, 2428, 2833
PflFI GACNNNGTC 2 cut(s) 130, 427
PflMI CCANNNNNTGG 1 cut(s) 1139
PfoI TCCNGGA 1 cut(s) 1000
Ple19I CGATCG 2 cut(s) 56, 387
PleI GAGTC 3 cut(s) 171, 235, 2609
PpsI GAGTC 3 cut(s) 171, 235, 2609
PpuMI RGGWCCY 2 cut(s) 1423, 1469
PshBI ATTAAT 1 cut(s) 1644
Psp124BI GAGCTC 1 cut(s) 2852
Psp1406I AACGTT 2 cut(s) 675, 2214
Psp5II RGGWCCY 2 cut(s) 1423, 1469
Psp6I CCWGG 8 cut(s) 291, 1000, 1120, 1471, 1717, 1796, 2043, 2139
PspFI CCCAGC 1 cut(s) 206
PspGI CCWGG 8 cut(s) 291, 1000, 1120, 1471, 1717, 1796, 2043, 2139
PspN4I GGNNCC 6 cut(s) 196, 823, 1425, 1803, 1926, 2544
PspPI GGNCC 7 cut(s) 126, 280, 728, 822, 1124, 1423, 1469
PspPPI RGGWCCY 2 cut(s) 1423, 1469
PsrI GAACNNNNNNTAC 2 cut(s) 1380, 1412
PstI CTGCAG 1 cut(s) 899
PsuI RGATCY 4 cut(s) 345, 985, 1924, 2700
PsyI GACNNNGTC 2 cut(s) 130, 427
PvuI CGATCG 2 cut(s) 56, 387
PvuII CAGCTG 1 cut(s) 206
RsaI GTAC 7 cut(s) 449, 870, 1014, 1334, 1405, 2184, 2392
RsaNI GTAC 7 cut(s) 448, 869, 1013, 1333, 1404, 2183, 2391
Rsr2I CGGWCCG 1 cut(s) 126
RsrII CGGWCCG 1 cut(s) 126
SacI GAGCTC 1 cut(s) 2852
SacII CCGCGG 1 cut(s) 78
SalI GTCGAC 2 cut(s) 229, 1554
SapI GCTCTTC 1 cut(s) 1512
SaqAI TTAA 7 cut(s) 906, 945, 1050, 1449, 1644, 1782, 2891
Sau96I GGNCC 7 cut(s) 126, 280, 728, 822, 1124, 1423, 1469
SchI GAGTC 3 cut(s) 172, 236, 2610
ScrFI CCNGG 9 cut(s) 293, 1002, 1122, 1473, 1719, 1798, 2045, 2141, 2411
SduI GDGCHC 3 cut(s) 1357, 1804, 2852
SexAI ACCWGGT 1 cut(s) 2043
SfaNI GCATC 4 cut(s) 730, 879, 1574, 1759
SfcI CTRYAG 1 cut(s) 895
Sfr303I CCGCGG 1 cut(s) 78
SfuI TTCGAA 2 cut(s) 308, 314
SgrAI CRCCGGYG 1 cut(s) 187
SgrBI CCGCGG 1 cut(s) 78
SinI GGWCC 6 cut(s) 126, 280, 822, 1124, 1423, 1469
SmlI CTYRAG 3 cut(s) 905, 2696, 2801
SmoI CTYRAG 3 cut(s) 905, 2696, 2801
SphI GCATGC 1 cut(s) 2387
SspMI CTAG 6 cut(s) 458, 872, 1875, 1983, 1989, 2069
SstI GAGCTC 1 cut(s) 2852
StyD4I CCNGG 9 cut(s) 291, 1000, 1120, 1471, 1717, 1796, 2043, 2139, 2409
StyI CCWWGG 2 cut(s) 2033, 2706
TaiI ACGT 7 cut(s) 678, 721, 2016, 2217, 2245, 2338, 2589
TaqII GACCGA 2 cut(s) 234, 2771
TatI WGTACW 4 cut(s) 447, 1332, 1403, 2390
TfiI GAWTC 9 cut(s) 241, 389, 515, 1217, 1832, 1904, 1952, 2428, 2833
Tru1I TTAA 7 cut(s) 906, 945, 1050, 1449, 1644, 1782, 2891
Tru9I TTAA 7 cut(s) 906, 945, 1050, 1449, 1644, 1782, 2891
TscAI CASTG 3 cut(s) 226, 650, 928
TseFI GTSAC 3 cut(s) 667, 2478, 2582
TseI GCWGC 5 cut(s) 203, 361, 1152, 2156, 2812
Tsp45I GTSAC 3 cut(s) 667, 2478, 2582
TspGWI ACGGA 4 cut(s) 82, 1659, 2126, 2817
TspRI CASTG 3 cut(s) 226, 650, 928
Tth111I GACNNNGTC 2 cut(s) 130, 427
Van91I CCANNNNNTGG 1 cut(s) 1139
Vha464I CTTAAG 1 cut(s) 905
VpaK11BI GGWCC 6 cut(s) 126, 280, 822, 1124, 1423, 1469
VspI ATTAAT 1 cut(s) 1644
XapI RAATTY 5 cut(s) 310, 1029, 1776, 2028, 2878
XceI RCATGY 1 cut(s) 2387
XmiI GTMKAC 3 cut(s) 230, 1555, 2088
XmnI GAANNNNTTC 3 cut(s) 1221, 1521, 2129
XspI CTAG 6 cut(s) 458, 872, 1875, 1983, 1989, 2069
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.