pycom12g21670

Serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr12
OG0004456
Physical Location & Seq
Reverse (-)
22798920 .. 22804790
5871 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom12g21670.7

Sequence Viewer

Length: 2535 bp
ATGAAGCACATTTTCAAGAAGCTTCACATAGGGAGCAACCACGACCCGACCCGATCGAACGAGGCCGCAGCGGCGGTCTCCTCCTCCTCCTCCGCCGTAGCCTCGCTGTCGTGCGCCTCCGATCACCGGGCGGCGTCAGGTCAGACGTCCGGAAATGCTCCGGCGAGTCCTTCGCCGACTCCTGCGGGAACCGTGGCAGCTGGGGGAGGTGGCACTGTGGAGACGACGACTGTGAATCGACCGGACTATATGATGTCGGAAGAGGAGTATCAGGTCCAGCTCGCCCTGGCGATCAGCGCTTCGAATTCGGACTTTAGGGATGATCCGGAGAAGGATCAGATCCGCGCGGCGACGCTGCTGAGCTTGGGCGGCAATCCGATCGATTCTTCGAGAGATAAGGTGGAGCCGGCGGCTGAGACCTTGTCGCGGCAGTATTGGGAGTACAATGTGCTAGACTATGAGGAGAAAGTGGTTGATGGATTTTATGATGTATATGGACTTTCCACAGATTCAGCAATTCAAGGAAAAATGCCTTCTCTCACAAATCTTGAAACAAACCTTGGGAGTTCTGGATTTGAAGTTTCATTAGTAAATCGAACAGTTGATCCTGTCTTGGAAGAGTTGGTGCAAATTGCACAGTGCATTGCTTTAGACTGTCCTGTCACCAACGTTGGTGTTTTGGTTCAGAGGCTTGCTGAACTCGTTAGTGAACGTATGGGTGGGCCTGTAAAGGATGCAAATGTTGTCTTAGCGAGATGGATGGAAAGAAGCACAGAGTTGAGGACATCTCTGCAAACAAGTGTATTGCCTATTGGGTCCATAACCATTGGCCTTTCCCGACATCGTGCTTTGCTTTTCAAGGTACTAGCTGACAATATCAAGATGCCCTGCAGACTGCTTAAGGGTGTTCATTACACTGGTGCTGAGGATGGTGCTGTTAATGTAATAAAGTTGGAGGATGACAGGGAGTTTTTGGTGGATCTAATGGCTGATCCTGGAACACTCATACCAACTGACACCCAAATTTCAAAGGATGCTACCTTTACGCCGTACAATCCAAACTTGAACAAACTTCCTACTTTCCATTCTTATATCGACACAGATATCGCTTACCCAGGACCAAAACCATTACATGGAGAGGGAAGCAGCCAAAACTCGGCAGTTGAAAGTAGTTCAGGTCTGGAGAGAAGACCAAGCTCAGATAAGGGAGAATCATTTCCAACATTTTCGGGTGCAAGTAGTGAGAGTGGAGTTGGTTCTTCAGGAATACCGAATAAAGCAACTCAGTTAGATCAGCTTTCCTCATCAGCCTCCAAAAAATCACGGTACAACAGAGGGAATCGTGGGGCTCATGCAATTGATGGTGGTGTGAGGATGAATGTAAATATAGTTCCATGTACTCAAAACAACTCTGAGGACCCAAAGAACCTTTTTGCTGATCTTAACCCATTCCAAATAAAAGGACCTGGGAAAGCTTCTATGTATAACAAACCTGCGGAGAATAAAGTTGAAGAGCTTCAGAGACAAAATAATAATCTTGCTTCTGGTCGACCTCCTGCATCTTTGATGTGGAAAAATAAATATGCTTTCAATGAAGTCCCTAAAAGAAAGGAGTATGATGGTATCCTCCCAAAAATTAATCGTGAGCCTAATGGTTACAATCCGTCATCTTCAGCTTCGACCAGCTCTACTGTATCTGAACAGGTCAAACCTGGTGGTTTCAATTCAACTGCTCATTCAAATATGTTTGACAGAGATGGTGATGCCAAAAATTTTAAAGGCGAACAACCCAGGGCTCCGAACTATTTACAGAACAATGAAACTGGTTTCAATGATCCTAGAAAGTTCACACATGACAGATTTATGGAATCCAATTTGAAACTGAAGGATCCAGAAAGTTGTAACTCATCATTTGATTCCATTTCAAGCAGGGTTGAATATCATTCTAGCACTAGCTCATCATTTGAGGATGTTGACGTAGGTGAATGTGAAATTCCGTGGGAAGACCTGGTTATTGGAGAAAGGATTGGACTAGGTTCATATGGTGAGGTATACCATGCTGATTGGCATGGCACGGAAGTTGCTGTGAAGAAGTTCTTAGACCAGGATTTCTCGGGTGCTGCTTTGGCTGAGTTCAAAAGAGAAGTACGAATAATGCGTAGGTTGCGTCATCCAAACGTTGTGCTTTTCATGGGGGCTGTTACACGTCCTCCAAACCTCTCTATCATTTCTGAGTTTCTTCCGAGGGGAAGTCTATATCGGATTATTCATCGTCCTCATTGTCAAATTGACGAGAAACGTAGAATAAAGATGGCTCTGGATGTGGCAAGGGGTATGAATTGCTTGCATGCTAGTACACCGACTATTGTTCACCGGGATTTGAAGTCTCCGAATCTGCTGGTTGATAAGAACTGGAATGTGAAGGTATCACGTCTTTTAACTGTTGGTTCTCTTGTTTCTTGTGTGCGATTTTGGTTTGTCACGCTTGAAACACAACACTTTCTTGTCGTCCAAATCAACTGCTGGAACGCCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000165 GO:0000186 GO:0001101 GO:0001932 GO:0001934 GO:0002229 GO:0002239 GO:0002831 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0004709 GO:0004712 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005768 GO:0005769 GO:0005783 GO:0005794 GO:0005798 GO:0005802 GO:0005829 GO:0005886 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0006952 GO:0007154 GO:0007165 GO:0008150 GO:0008152 GO:0008219 GO:0009414 GO:0009415 GO:0009605 GO:0009607 GO:0009617 GO:0009620 GO:0009628 GO:0009719 GO:0009723 GO:0009725 GO:0009787 GO:0009788 GO:0009893 GO:0009966 GO:0009967 GO:0009968 GO:0009987 GO:0010033 GO:0010035 GO:0010562 GO:0010604 GO:0010646 GO:0010647 GO:0010648 GO:0012505 GO:0012506 GO:0012510 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019220 GO:0019222 GO:0019538 GO:0023014 GO:0023051 GO:0023052 GO:0023056 GO:0023057 GO:0030133 GO:0030135 GO:0030136 GO:0030140 GO:0030658 GO:0030659 GO:0030660 GO:0030662 GO:0030665 GO:0031090 GO:0031323 GO:0031325 GO:0031347 GO:0031399 GO:0031401 GO:0031410 GO:0031982 GO:0031984 GO:0032101 GO:0032147 GO:0032268 GO:0032270 GO:0033674 GO:0035556 GO:0036211 GO:0042221 GO:0042325 GO:0042327 GO:0043085 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043405 GO:0043406 GO:0043408 GO:0043410 GO:0043412 GO:0043549 GO:0043900 GO:0044093 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044431 GO:0044433 GO:0044444 GO:0044446 GO:0044464 GO:0045859 GO:0045860 GO:0045937 GO:0046777 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048583 GO:0048584 GO:0048585 GO:0050789 GO:0050790 GO:0050794 GO:0050896 GO:0051171 GO:0051173 GO:0051174 GO:0051246 GO:0051247 GO:0051338 GO:0051347 GO:0051704 GO:0051707 GO:0051716 GO:0060255 GO:0065007 GO:0065009 GO:0071704 GO:0071900 GO:0071902 GO:0071944 GO:0080090 GO:0080134 GO:0097708 GO:0098542 GO:0098588 GO:0098791 GO:0098805 GO:0140096 GO:1900150 GO:1900424 GO:1901419 GO:1901420 GO:1901564 GO:1901700 GO:1902531 GO:1902533 GO:1905957 GO:1905958 GO:2000031 GO:2001023 GO:2001038
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EDR1 Pkinase_Tyr

Protein Analysis

845

Amino Acids

92.55

Weight (kDa)

6.1

Isoelectric Point (pI)

42.16

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 659
AatII GACGTC 1 cut(s) 149
Acc36I ACCTGC 1 cut(s) 1501
AccB7I CCANNNNNTGG 1 cut(s) 1133
AccI GTMKAC 2 cut(s) 1549, 2052
AccII CGCG 3 cut(s) 345, 347, 427
AccIII TCCGGA 2 cut(s) 149, 325
AclI AACGTT 2 cut(s) 669, 2178
AclWI GGATC 9 cut(s) 317, 334, 342, 599, 986, 987, 1829, 1883, 1896
AcsI RAATTY 4 cut(s) 304, 1023, 1771, 1992
AcuI CTGAAG 4 cut(s) 1245, 1502, 1656, 1904
AcyI GRCGYC 2 cut(s) 134, 146
AfaI GTAC 7 cut(s) 443, 864, 1052, 1328, 1399, 2148, 2356
AfeI AGCGCT 1 cut(s) 298
AfiI CCNNNNNNNGG 4 cut(s) 126, 426, 1133, 1156
AflII CTTAAG 1 cut(s) 899
AflIII ACRYGT 1 cut(s) 2204
AjiI CACGTC 2 cut(s) 2207, 2432
AjnI CCWGG 8 cut(s) 285, 994, 1114, 1465, 1711, 1790, 2007, 2103
AjuI GAANNNNNNNTTGG 2 cut(s) 543, 575
AloI GAACNNNNNNTCC 2 cut(s) 589, 621
Alw26I GTCTC 5 cut(s) 82, 215, 410, 1516, 2391
AlwI GGATC 9 cut(s) 317, 334, 342, 599, 986, 987, 1829, 1883, 1896
Ama87I CYCGRG 1 cut(s) 2113
Aor13HI TCCGGA 2 cut(s) 149, 325
Aor51HI AGCGCT 1 cut(s) 298
AoxI GGCC 3 cut(s) 63, 722, 829
ApeKI GCWGC 5 cut(s) 68, 197, 355, 1146, 2120
ApoI RAATTY 4 cut(s) 304, 1023, 1771, 1992
AseI ATTAAT 1 cut(s) 1638
Asp700I GAANNNNTTC 3 cut(s) 1215, 1515, 2093
AspLEI GCGC 3 cut(s) 116, 299, 347
AspS9I GGNCC 6 cut(s) 274, 722, 816, 1118, 1417, 1463
AsuC2I CCSGG 2 cut(s) 128, 2375
AsuHPI GGTGA 6 cut(s) 116, 655, 1772, 1994, 2057, 2363
AsuII TTCGAA 1 cut(s) 302
AvaI CYCGRG 1 cut(s) 2113
AvaII GGWCC 5 cut(s) 274, 816, 1118, 1417, 1463
BamHI GGATCC 1 cut(s) 1888
BanII GRGCYC 2 cut(s) 1351, 1798
BbsI GAAGAC 2 cut(s) 1195, 2010
BbvCI CCTCAGC 1 cut(s) 924
BbvI GCAGC 5 cut(s) 80, 209, 342, 1158, 2107
BccI CCATC 8 cut(s) 470, 750, 754, 923, 1355, 1613, 1751, 2305
BceAI ACGGC 2 cut(s) 80, 1033
BcgI CGANNNNNNTGC 2 cut(s) 361, 395
BciT130I CCWGG 8 cut(s) 287, 996, 1116, 1467, 1713, 1792, 2009, 2105
BciVI GTATCC 1 cut(s) 1634
BcnI CCSGG 2 cut(s) 128, 2375
BcoDI GTCTC 5 cut(s) 82, 215, 410, 1516, 2391
BfaI CTAG 7 cut(s) 452, 866, 1839, 1947, 1953, 2033, 2352
BfmI CTRYAG 1 cut(s) 889
BfoI RGCGCY 1 cut(s) 300
BfrI CTTAAG 1 cut(s) 899
BfuAI ACCTGC 1 cut(s) 1501
BfuI GTATCC 1 cut(s) 1634
BglI GCCNNNNNGGC 1 cut(s) 71
BlpI GCTNAGC 1 cut(s) 359
Bme18I GGWCC 5 cut(s) 274, 816, 1118, 1417, 1463
BmeT110I CYCGRG 1 cut(s) 2113
BmgBI CACGTC 2 cut(s) 2207, 2432
BmgT120I GGNCC 6 cut(s) 274, 722, 816, 1118, 1417, 1463
BmiI GGNNCC 6 cut(s) 190, 405, 817, 1419, 1797, 1890
BmsI GCATC 5 cut(s) 724, 873, 1024, 1568, 1753
BpiI GAAGAC 2 cut(s) 1195, 2010
BplI GAGNNNNNCTC 2 cut(s) 772, 804
BpmI CTGGAG 1 cut(s) 1202
Bpu10I CCTNAGC 1 cut(s) 924
Bpu1102I GCTNAGC 1 cut(s) 359
Bpu14I TTCGAA 1 cut(s) 302
BpuMI CCSGG 2 cut(s) 128, 2375
Bsa29I ATCGAT 1 cut(s) 381
BsaHI GRCGYC 2 cut(s) 134, 146
BsaI GGTCTC 2 cut(s) 82, 410
BsaJI CCNNGG 9 cut(s) 192, 285, 559, 1114, 1466, 1790, 1791, 1997, 2243
BsaWI WCCGGW 3 cut(s) 149, 241, 325
BsaXI ACNNNNNCTCC 2 cut(s) 2194, 2224
Bsc4I CCNNNNNNNGG 4 cut(s) 126, 426, 1133, 1156
Bse118I RCCGGY 1 cut(s) 406
Bse1I ACTGG 3 cut(s) 922, 1828, 2417
Bse3DI GCAATG 1 cut(s) 642
BseAI TCCGGA 2 cut(s) 149, 325
BseBI CCWGG 8 cut(s) 287, 996, 1116, 1467, 1713, 1792, 2009, 2105
BseCI ATCGAT 1 cut(s) 381
BseDI CCNNGG 9 cut(s) 192, 285, 559, 1114, 1466, 1790, 1791, 1997, 2243
BseLI CCNNNNNNNGG 4 cut(s) 126, 426, 1133, 1156
BseMI GCAATG 1 cut(s) 642
BseMII CTCAG 8 cut(s) 350, 405, 915, 1212, 1298, 1404, 2121, 2223
BseNI ACTGG 3 cut(s) 922, 1828, 2417
BseRI GAGGAG 6 cut(s) 70, 73, 76, 79, 278, 476
BseXI GCAGC 5 cut(s) 80, 209, 342, 1158, 2107
BseYI CCCAGC 1 cut(s) 200
Bsh1236I CGCG 3 cut(s) 345, 347, 427
Bsh1285I CGRYCG 3 cut(s) 56, 242, 381
BshFI GGCC 3 cut(s) 65, 724, 831
BshVI ATCGAT 1 cut(s) 381
BsiEI CGRYCG 3 cut(s) 56, 242, 381
BsiHKCI CYCGRG 1 cut(s) 2113
BsiSI CCGG 7 cut(s) 127, 150, 161, 242, 326, 407, 2374
BslFI GGGAC 1 cut(s) 1583
BslI CCNNNNNNNGG 4 cut(s) 126, 426, 1133, 1156
BsmAI GTCTC 5 cut(s) 82, 215, 410, 1516, 2391
BsmBI CGTCTC 1 cut(s) 215
BsmFI GGGAC 1 cut(s) 1583
BsnI GGCC 3 cut(s) 65, 724, 831
Bso31I GGTCTC 2 cut(s) 82, 410
BsoBI CYCGRG 1 cut(s) 2113
Bsp119I TTCGAA 1 cut(s) 302
Bsp1286I GDGCHC 2 cut(s) 1351, 1798
Bsp13I TCCGGA 2 cut(s) 149, 325
Bsp1720I GCTNAGC 1 cut(s) 359
BspANI GGCC 3 cut(s) 65, 724, 831
BspCNI CTCAG 8 cut(s) 351, 406, 916, 1211, 1297, 1405, 2122, 2224
BspDI ATCGAT 1 cut(s) 381
BspEI TCCGGA 2 cut(s) 149, 325
BspFNI CGCG 3 cut(s) 345, 347, 427
BspLI GGNNCC 6 cut(s) 190, 405, 817, 1419, 1797, 1890
BspMAI CTGCAG 1 cut(s) 893
BspMI ACCTGC 1 cut(s) 1501
BspPI GGATC 9 cut(s) 317, 334, 342, 599, 986, 987, 1829, 1883, 1896
BspQI GCTCTTC 1 cut(s) 1506
BspT104I TTCGAA 1 cut(s) 302
BspTI CTTAAG 1 cut(s) 899
BspTNI GGTCTC 2 cut(s) 82, 410
BsrDI GCAATG 1 cut(s) 642
BsrFI RCCGGY 1 cut(s) 406
BsrI ACTGG 3 cut(s) 922, 1828, 2417
BssAI RCCGGY 1 cut(s) 406
BssECI CCNNGG 9 cut(s) 192, 285, 559, 1114, 1466, 1790, 1791, 1997, 2243
BssNAI GTATAC 1 cut(s) 2053
BssNI GRCGYC 2 cut(s) 134, 146
BssT1I CCWWGG 1 cut(s) 559
Bst1107I GTATAC 1 cut(s) 2053
Bst2UI CCWGG 8 cut(s) 287, 996, 1116, 1467, 1713, 1792, 2009, 2105
Bst4CI ACNGT 9 cut(s) 193, 217, 232, 601, 639, 656, 1326, 1693, 2443
Bst6I CTCTTC 3 cut(s) 255, 612, 1506
BstACI GRCGYC 2 cut(s) 134, 146
BstAFI CTTAAG 1 cut(s) 899
BstBI TTCGAA 1 cut(s) 302
BstC8I GCNNGC 5 cut(s) 282, 408, 693, 2345, 2349
BstDSI CCRYGG 2 cut(s) 192, 1997
BstFNI CGCG 3 cut(s) 345, 347, 427
BstH2I RGCGCY 1 cut(s) 300
BstHHI GCGC 3 cut(s) 116, 299, 347
BstMAI GTCTC 5 cut(s) 82, 215, 410, 1516, 2391
BstMCI CGRYCG 3 cut(s) 56, 242, 381
BstMWI GCNNNNNNNGC 5 cut(s) 71, 296, 369, 2126, 2164
BstNI CCWGG 8 cut(s) 287, 996, 1116, 1467, 1713, 1792, 2009, 2105
BstNSI RCATGY 1 cut(s) 2351
BstSFI CTRYAG 1 cut(s) 889
BstUI CGCG 3 cut(s) 345, 347, 427
BstV1I GCAGC 5 cut(s) 80, 209, 342, 1158, 2107
BstV2I GAAGAC 2 cut(s) 1195, 2010
BstX2I RGATCY 3 cut(s) 339, 979, 1888
BstYI RGATCY 3 cut(s) 339, 979, 1888
BstZ17I GTATAC 1 cut(s) 2053
Bsu15I ATCGAT 1 cut(s) 381
BsuI GTATCC 1 cut(s) 1634
BsuRI GGCC 3 cut(s) 65, 724, 831
BsuTUI ATCGAT 1 cut(s) 381
BtgI CCRYGG 2 cut(s) 192, 1997
BtrI CACGTC 2 cut(s) 2207, 2432
BtsIMutI CAGTG 3 cut(s) 213, 644, 915
BveI ACCTGC 1 cut(s) 1501
Cac8I GCNNGC 5 cut(s) 282, 408, 693, 2345, 2349
CfoI GCGC 3 cut(s) 116, 299, 347
Cfr10I RCCGGY 1 cut(s) 406
Cfr13I GGNCC 6 cut(s) 274, 722, 816, 1118, 1417, 1463
ClaI ATCGAT 1 cut(s) 381
CseI GACGC 3 cut(s) 123, 361, 2156
CsiI ACCWGGT 2 cut(s) 1711, 2007
Csp6I GTAC 7 cut(s) 442, 863, 1051, 1327, 1398, 2147, 2355
CspCI CAANNNNNGTGG 2 cut(s) 1696, 1731
CviAII CATG 8 cut(s) 1133, 1352, 1395, 1853, 2057, 2069, 2191, 2348
CviQI GTAC 7 cut(s) 442, 863, 1051, 1327, 1398, 2147, 2355
DraI TTTAAA 1 cut(s) 1777
DrdI GACNNNNNNGTC 1 cut(s) 659
DseDI GACNNNNNNGTC 1 cut(s) 659
Eam1104I CTCTTC 3 cut(s) 255, 612, 1506
EarI CTCTTC 3 cut(s) 255, 612, 1506
EciI GGCGGA 1 cut(s) 82
Eco130I CCWWGG 1 cut(s) 559
Eco24I GRGCYC 2 cut(s) 1351, 1798
Eco31I GGTCTC 2 cut(s) 82, 410
Eco32I GATATC 1 cut(s) 1105
Eco47I GGWCC 5 cut(s) 274, 816, 1118, 1417, 1463
Eco47III AGCGCT 1 cut(s) 298
Eco57I CTGAAG 4 cut(s) 1245, 1502, 1656, 1904
Eco88I CYCGRG 1 cut(s) 2113
EcoO109I RGGNCCY 2 cut(s) 1417, 1463
EcoRI GAATTC 1 cut(s) 304
EcoRII CCWGG 8 cut(s) 285, 994, 1114, 1465, 1711, 1790, 2007, 2103
EcoRV GATATC 1 cut(s) 1105
EcoT14I CCWWGG 1 cut(s) 559
EcoT38I GRGCYC 2 cut(s) 1351, 1798
ErhI CCWWGG 1 cut(s) 559
Esp3I CGTCTC 1 cut(s) 215
FaeI CATG 8 cut(s) 1136, 1355, 1398, 1856, 2060, 2072, 2194, 2351
FalI AAGNNNNNCTT 2 cut(s) 2081, 2113
FaqI GGGAC 1 cut(s) 1583
FatI CATG 8 cut(s) 1132, 1351, 1394, 1852, 2056, 2068, 2190, 2347
FauI CCCGC 1 cut(s) 178
FauNDI CATATG 1 cut(s) 2041
FblI GTMKAC 2 cut(s) 1549, 2052
FriOI GRGCYC 2 cut(s) 1351, 1798
FspBI CTAG 7 cut(s) 452, 866, 1839, 1947, 1953, 2033, 2352
GlaI GCGC 3 cut(s) 115, 298, 346
GsaI CCCAGC 1 cut(s) 204
GsuI CTGGAG 1 cut(s) 1202
HaeII RGCGCY 1 cut(s) 300
HaeIII GGCC 3 cut(s) 65, 724, 831
HapII CCGG 7 cut(s) 127, 150, 161, 242, 326, 407, 2374
HgaI GACGC 3 cut(s) 123, 361, 2156
HhaI GCGC 3 cut(s) 116, 299, 347
Hin1I GRCGYC 2 cut(s) 134, 146
Hin1II CATG 8 cut(s) 1136, 1355, 1398, 1856, 2060, 2072, 2194, 2351
Hin6I GCGC 3 cut(s) 114, 297, 345
HinP1I GCGC 3 cut(s) 114, 297, 345
HincII GTYRAC 2 cut(s) 1550, 1975
HindII GTYRAC 2 cut(s) 1550, 1975
HindIII AAGCTT 2 cut(s) 20, 1473
HpaII CCGG 7 cut(s) 127, 150, 161, 242, 326, 407, 2374
HphI GGTGA 6 cut(s) 116, 655, 1772, 1994, 2057, 2363
Hpy166II GTNNAC 7 cut(s) 710, 1550, 1848, 1975, 2053, 2357, 2371
Hpy8I GTNNAC 7 cut(s) 710, 1550, 1848, 1975, 2053, 2357, 2371
Hpy99I CGWCG 2 cut(s) 229, 355
HpyAV CCTTC 5 cut(s) 180, 325, 543, 1879, 2416
HpyCH4III ACNGT 9 cut(s) 193, 217, 232, 601, 639, 656, 1326, 1693, 2443
HpyCH4IV ACGT 8 cut(s) 146, 669, 712, 1977, 2178, 2206, 2299, 2431
HpyF10VI GCNNNNNNNGC 5 cut(s) 71, 296, 369, 2126, 2164
HpySE526I ACGT 8 cut(s) 146, 669, 712, 1977, 2178, 2206, 2299, 2431
Hsp92I GRCGYC 2 cut(s) 134, 146
Hsp92II CATG 8 cut(s) 1136, 1355, 1398, 1856, 2060, 2072, 2194, 2351
HspAI GCGC 3 cut(s) 114, 297, 345
Kpn2I TCCGGA 2 cut(s) 149, 325
KroI GCCGGC 1 cut(s) 406
KroNI GCCGGC 1 cut(s) 408
LguI GCTCTTC 1 cut(s) 1506
LmnI GCTCC 4 cut(s) 33, 163, 403, 1801
Lsp1109I GCAGC 5 cut(s) 80, 209, 342, 1158, 2107
LweI GCATC 5 cut(s) 724, 873, 1024, 1568, 1753
MabI ACCWGGT 2 cut(s) 1711, 2007
MaeI CTAG 7 cut(s) 452, 866, 1839, 1947, 1953, 2033, 2352
MaeII ACGT 8 cut(s) 146, 669, 712, 1977, 2178, 2206, 2299, 2431
MaeIII GTNAC 5 cut(s) 661, 1655, 1901, 2200, 2479
MfeI CAATTG 1 cut(s) 1356
MflI RGATCY 3 cut(s) 339, 979, 1888
MhlI GDGCHC 2 cut(s) 1351, 1798
MlyI GAGTC 2 cut(s) 172, 175
MmeI TCCRAC 3 cut(s) 237, 933, 1244
MroI TCCGGA 2 cut(s) 149, 325
MroNI GCCGGC 1 cut(s) 406
MroXI GAANNNNTTC 3 cut(s) 1215, 1515, 2093
MseI TTAA 6 cut(s) 900, 939, 1443, 1638, 1776, 2438
MspA1I CMGCKG 2 cut(s) 71, 200
MspCI CTTAAG 1 cut(s) 899
MspI CCGG 7 cut(s) 127, 150, 161, 242, 326, 407, 2374
MunI CAATTG 1 cut(s) 1356
MvaI CCWGG 8 cut(s) 287, 996, 1116, 1467, 1713, 1792, 2009, 2105
MvnI CGCG 3 cut(s) 345, 347, 427
MwoI GCNNNNNNNGC 5 cut(s) 71, 296, 369, 2126, 2164
NaeI GCCGGC 1 cut(s) 408
NciI CCSGG 2 cut(s) 128, 2375
NdeI CATATG 1 cut(s) 2041
NgoMIV GCCGGC 1 cut(s) 406
NlaIII CATG 8 cut(s) 1136, 1355, 1398, 1856, 2060, 2072, 2194, 2351
NlaIV GGNNCC 6 cut(s) 190, 405, 817, 1419, 1797, 1890
NmeAIII GCCGAG 1 cut(s) 1136
NmuCI GTSAC 2 cut(s) 661, 2479
NspI RCATGY 1 cut(s) 2351
NspV TTCGAA 1 cut(s) 302
PaeI GCATGC 1 cut(s) 2351
PasI CCCWGGG 1 cut(s) 1791
PciSI GCTCTTC 1 cut(s) 1506
PcsI WCGNNNNNNNCGW 1 cut(s) 2155
PdiI GCCGGC 1 cut(s) 408
PdmI GAANNNNTTC 3 cut(s) 1215, 1515, 2093
PfeI GAWTC 8 cut(s) 235, 383, 509, 1211, 1339, 1868, 1916, 2392
PflFI GACNNNGTC 1 cut(s) 421
PflMI CCANNNNNTGG 1 cut(s) 1133
PfoI TCCNGGA 1 cut(s) 994
Ple19I CGATCG 2 cut(s) 56, 381
PleI GAGTC 2 cut(s) 172, 174
PpsI GAGTC 2 cut(s) 172, 174
PpuMI RGGWCCY 2 cut(s) 1417, 1463
PshBI ATTAAT 1 cut(s) 1638
Psp1406I AACGTT 2 cut(s) 669, 2178
Psp5II RGGWCCY 2 cut(s) 1417, 1463
Psp6I CCWGG 8 cut(s) 285, 994, 1114, 1465, 1711, 1790, 2007, 2103
PspFI CCCAGC 1 cut(s) 200
PspGI CCWGG 8 cut(s) 285, 994, 1114, 1465, 1711, 1790, 2007, 2103
PspN4I GGNNCC 6 cut(s) 190, 405, 817, 1419, 1797, 1890
PspPI GGNCC 6 cut(s) 274, 722, 816, 1118, 1417, 1463
PspPPI RGGWCCY 2 cut(s) 1417, 1463
PsrI GAACNNNNNNTAC 2 cut(s) 1374, 1406
PstI CTGCAG 1 cut(s) 893
PsuI RGATCY 3 cut(s) 339, 979, 1888
PsyI GACNNNGTC 1 cut(s) 421
PvuI CGATCG 2 cut(s) 56, 381
PvuII CAGCTG 1 cut(s) 200
RsaI GTAC 7 cut(s) 443, 864, 1052, 1328, 1399, 2148, 2356
RsaNI GTAC 7 cut(s) 442, 863, 1051, 1327, 1398, 2147, 2355
SalI GTCGAC 1 cut(s) 1548
SapI GCTCTTC 1 cut(s) 1506
SaqAI TTAA 6 cut(s) 900, 939, 1443, 1638, 1776, 2438
Sau96I GGNCC 6 cut(s) 274, 722, 816, 1118, 1417, 1463
SchI GAGTC 2 cut(s) 172, 175
SduI GDGCHC 2 cut(s) 1351, 1798
SexAI ACCWGGT 2 cut(s) 1711, 2007
SfaNI GCATC 5 cut(s) 724, 873, 1024, 1568, 1753
SfcI CTRYAG 1 cut(s) 889
SfuI TTCGAA 1 cut(s) 302
SinI GGWCC 5 cut(s) 274, 816, 1118, 1417, 1463
SmlI CTYRAG 1 cut(s) 899
SmoI CTYRAG 1 cut(s) 899
SphI GCATGC 1 cut(s) 2351
SspMI CTAG 7 cut(s) 452, 866, 1839, 1947, 1953, 2033, 2352
StyI CCWWGG 1 cut(s) 559
TaaI ACNGT 9 cut(s) 193, 217, 232, 601, 639, 656, 1326, 1693, 2443
TaiI ACGT 8 cut(s) 149, 672, 715, 1980, 2181, 2209, 2302, 2434
TaqI TCGA 9 cut(s) 56, 238, 302, 381, 389, 595, 1095, 1549, 1679
TatI WGTACW 3 cut(s) 441, 1397, 2354
TauI GCSGC 7 cut(s) 68, 74, 134, 350, 372, 413, 430
TfiI GAWTC 8 cut(s) 235, 383, 509, 1211, 1339, 1868, 1916, 2392
Tru1I TTAA 6 cut(s) 900, 939, 1443, 1638, 1776, 2438
Tru9I TTAA 6 cut(s) 900, 939, 1443, 1638, 1776, 2438
TscAI CASTG 3 cut(s) 220, 644, 922
TseFI GTSAC 2 cut(s) 661, 2479
TseI GCWGC 5 cut(s) 68, 197, 355, 1146, 2120
Tsp45I GTSAC 2 cut(s) 661, 2479
TspGWI ACGGA 3 cut(s) 1653, 1986, 2090
TspRI CASTG 3 cut(s) 220, 644, 922
Tth111I GACNNNGTC 1 cut(s) 421
Van91I CCANNNNNTGG 1 cut(s) 1133
Vha464I CTTAAG 1 cut(s) 899
VpaK11BI GGWCC 5 cut(s) 274, 816, 1118, 1417, 1463
VspI ATTAAT 1 cut(s) 1638
XapI RAATTY 4 cut(s) 304, 1023, 1771, 1992
XceI RCATGY 1 cut(s) 2351
XmiI GTMKAC 2 cut(s) 1549, 2052
XmnI GAANNNNTTC 3 cut(s) 1215, 1515, 2093
XspI CTAG 7 cut(s) 452, 866, 1839, 1947, 1953, 2033, 2352
ZraI GACGTC 1 cut(s) 147
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.