MD04G1220300.v1.1

Belongs to the eukaryotic ribosomal protein eS24 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr04
OG0004025
Physical Location & Seq
Reverse (-)
30141535 .. 30143692
2158 bp
Loading structure...
UTR
Exon/CDS
Intron
MD04G1220300.v1.1.491

Sequence Viewer

Length: 411 bp
ATGGCGGACAAGGCAGTCACCATCCGAACCAGGAAGTTCATGACCAACCGCCTTCTCTCTCGCAAGCAATTCGTCATCGATGTTCTTCATCCAGGAAGGCCAAATGTTTCAAAGGCTGAGCTGAAGGAGAAGCTTGCAAGGCTCTATGAGGTCAAGGACCCGAACGCAATCTTTGTGTTCAAGTTCAGGACTCACTTTGGTGGTGGCAAATCTACTGGATTTGGATTGATTTATGACTCTGTTGAAAATGCGAAGAAGTACGAGCCCAAGTACAGGTTGATCAGGAACGGACTTGATACCAAGGTAGAGAAGTCAAGGAAGCAGTTGAAGGAGAGAAAGAACAGAGCCAAGAAGATCCGAGGAGTAAAGAAGACCAAGGCATCAGATGCTGCTAAGTCAAAGAAGAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03010 map03010
Pfam Domains
Protein Families
Ribosomal_S24e

Protein Analysis

137

Amino Acids

15.71

Weight (kDa)

10.64

Isoelectric Point (pI)

10.05

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribosomal_S24e PF01282 6 - 97 6.8e-39 Ribosomal protein S24e
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 14
AciI CCGC 2 cut(s) 5, 49
AclWI GGATC 1 cut(s) 349
AcuI CTGAAG 1 cut(s) 143
AfaI GTAC 2 cut(s) 260, 272
AfiI CCNNNNNNNGG 1 cut(s) 273
AgsI TTSAA 4 cut(s) 111, 181, 245, 328
AjnI CCWGG 2 cut(s) 29, 91
AleI CACNNNNGTG 1 cut(s) 198
AluBI AGCT 2 cut(s) 121, 133
AluI AGCT 2 cut(s) 121, 133
AlwI GGATC 1 cut(s) 349
AlwNI CAGNNNCTG 1 cut(s) 389
AoxI GGCC 1 cut(s) 98
ApeKI GCWGC 1 cut(s) 389
AspS9I GGNCC 1 cut(s) 157
AsuHPI GGTGA 1 cut(s) 10
AvaII GGWCC 1 cut(s) 157
BaeI ACNNNNGTAYC 2 cut(s) 288, 321
BanII GRGCYC 1 cut(s) 267
BbsI GAAGAC 1 cut(s) 377
BbvI GCAGC 1 cut(s) 376
BccI CCATC 1 cut(s) 29
BcgI CGANNNNNNTGC 2 cut(s) 52, 86
BciT130I CCWGG 2 cut(s) 31, 93
BclI TGATCA 1 cut(s) 279
BisI GCNGC 1 cut(s) 390
BlpI GCTNAGC 1 cut(s) 117
BlsI GCNGC 1 cut(s) 391
Bme1390I CCNGG 2 cut(s) 31, 93
Bme18I GGWCC 1 cut(s) 157
BmgT120I GGNCC 1 cut(s) 157
BmiI GGNNCC 1 cut(s) 159
BmrFI CCNGG 2 cut(s) 31, 93
BmsI GCATC 2 cut(s) 376, 389
BpiI GAAGAC 1 cut(s) 377
Bpu1102I GCTNAGC 1 cut(s) 117
Bsa29I ATCGAT 1 cut(s) 78
BsaJI CCNNGG 3 cut(s) 300, 358, 375
Bsc4I CCNNNNNNNGG 1 cut(s) 273
Bse1I ACTGG 1 cut(s) 220
BseBI CCWGG 2 cut(s) 31, 93
BseCI ATCGAT 1 cut(s) 78
BseDI CCNNGG 3 cut(s) 300, 358, 375
BseGI GGATG 2 cut(s) 21, 88
BseLI CCNNNNNNNGG 1 cut(s) 273
BseMII CTCAG 1 cut(s) 108
BseNI ACTGG 1 cut(s) 220
BseRI GAGGAG 1 cut(s) 375
BseXI GCAGC 1 cut(s) 376
BshFI GGCC 1 cut(s) 100
BshVI ATCGAT 1 cut(s) 78
BslI CCNNNNNNNGG 1 cut(s) 273
BsnI GGCC 1 cut(s) 100
Bsp1286I GDGCHC 1 cut(s) 267
Bsp143I GATC 2 cut(s) 279, 354
Bsp1720I GCTNAGC 1 cut(s) 117
BspACI CCGC 2 cut(s) 5, 49
BspANI GGCC 1 cut(s) 100
BspCNI CTCAG 1 cut(s) 109
BspDI ATCGAT 1 cut(s) 78
BspHI TCATGA 1 cut(s) 39
BspLI GGNNCC 1 cut(s) 159
BspPI GGATC 1 cut(s) 349
BsrI ACTGG 1 cut(s) 220
BssECI CCNNGG 3 cut(s) 300, 358, 375
BssMI GATC 2 cut(s) 279, 354
BssT1I CCWWGG 2 cut(s) 300, 375
Bst2UI CCWGG 2 cut(s) 31, 93
BstAPI GCANNNNNTGC 1 cut(s) 386
BstC8I GCNNGC 2 cut(s) 65, 135
BstDEI CTNAG 2 cut(s) 117, 393
BstF5I GGATG 2 cut(s) 21, 88
BstKTI GATC 2 cut(s) 282, 357
BstMBI GATC 2 cut(s) 279, 354
BstMWI GCNNNNNNNGC 3 cut(s) 11, 139, 386
BstNI CCWGG 2 cut(s) 31, 93
BstSCI CCNGG 2 cut(s) 29, 91
BstV1I GCAGC 1 cut(s) 376
BstV2I GAAGAC 1 cut(s) 377
BstX2I RGATCY 1 cut(s) 354
BstYI RGATCY 1 cut(s) 354
Bsu15I ATCGAT 1 cut(s) 78
BsuRI GGCC 1 cut(s) 100
BsuTUI ATCGAT 1 cut(s) 78
BtsCI GGATG 2 cut(s) 21, 88
Cac8I GCNNGC 2 cut(s) 65, 135
CaiI CAGNNNCTG 1 cut(s) 389
CciI TCATGA 1 cut(s) 39
Cfr13I GGNCC 1 cut(s) 157
ClaI ATCGAT 1 cut(s) 78
Csp6I GTAC 2 cut(s) 259, 271
CviAII CATG 1 cut(s) 40
CviJI RGCY 7 cut(s) 100, 116, 121, 133, 142, 265, 347
CviKI_1 RGCY 7 cut(s) 100, 116, 121, 133, 142, 265, 347
CviQI GTAC 2 cut(s) 259, 271
DdeI CTNAG 2 cut(s) 117, 393
DpnI GATC 2 cut(s) 281, 356
DpnII GATC 2 cut(s) 279, 354
DrdI GACNNNNNNGTC 1 cut(s) 14
DseDI GACNNNNNNGTC 1 cut(s) 14
EciI GGCGGA 1 cut(s) 20
Eco130I CCWWGG 2 cut(s) 300, 375
Eco24I GRGCYC 1 cut(s) 267
Eco47I GGWCC 1 cut(s) 157
Eco57I CTGAAG 1 cut(s) 143
EcoO109I RGGNCCY 1 cut(s) 157
EcoRII CCWGG 2 cut(s) 29, 91
EcoT14I CCWWGG 2 cut(s) 300, 375
EcoT38I GRGCYC 1 cut(s) 267
ErhI CCWWGG 2 cut(s) 300, 375
FaeI CATG 1 cut(s) 43
FaiI YATR 3 cut(s) 41, 147, 234
FatI CATG 1 cut(s) 39
FbaI TGATCA 1 cut(s) 279
Fnu4HI GCNGC 1 cut(s) 390
FokI GGATG 2 cut(s) 8, 75
FriOI GRGCYC 1 cut(s) 267
Fsp4HI GCNGC 1 cut(s) 390
GluI GCNGC 1 cut(s) 390
HaeIII GGCC 1 cut(s) 100
Hin1II CATG 1 cut(s) 43
HindIII AAGCTT 1 cut(s) 131
HinfI GANTC 2 cut(s) 190, 236
HphI GGTGA 1 cut(s) 10
Hpy188I TCNGA 3 cut(s) 26, 359, 385
Hpy188III TCNNGA 3 cut(s) 40, 187, 283
HpyAV CCTTC 4 cut(s) 62, 90, 118, 322
HpyCH4V TGCA 1 cut(s) 137
HpyF10VI GCNNNNNNNGC 3 cut(s) 11, 139, 386
HpyF3I CTNAG 2 cut(s) 117, 393
Hsp92II CATG 1 cut(s) 43
Ksp22I TGATCA 1 cut(s) 279
Kzo9I GATC 2 cut(s) 279, 354
LpnPI CCDG 8 cut(s) 16, 43, 78, 105, 172, 201, 259, 268
Lsp1109I GCAGC 1 cut(s) 376
LweI GCATC 2 cut(s) 376, 389
MaeIII GTNAC 1 cut(s) 16
MalI GATC 2 cut(s) 281, 356
MboI GATC 2 cut(s) 279, 354
MboII GAAGA 4 cut(s) 77, 265, 364, 382
MflI RGATCY 1 cut(s) 354
MhlI GDGCHC 1 cut(s) 267
MluCI AATT 1 cut(s) 68
MlyI GAGTC 2 cut(s) 184, 230
MnlI CCTC 2 cut(s) 142, 353
MslI CAYNNNNRTG 1 cut(s) 198
MspR9I CCNGG 2 cut(s) 31, 93
MvaI CCWGG 2 cut(s) 31, 93
MwoI GCNNNNNNNGC 3 cut(s) 11, 139, 386
NdeII GATC 2 cut(s) 279, 354
NlaIII CATG 1 cut(s) 43
NlaIV GGNNCC 1 cut(s) 159
NmuCI GTSAC 1 cut(s) 16
OliI CACNNNNGTG 1 cut(s) 198
PagI TCATGA 1 cut(s) 39
PfoI TCCNGGA 1 cut(s) 91
PkrI GCNGC 1 cut(s) 391
PleI GAGTC 2 cut(s) 184, 230
PpsI GAGTC 2 cut(s) 184, 230
PpuMI RGGWCCY 1 cut(s) 157
Psp5II RGGWCCY 1 cut(s) 157
Psp6I CCWGG 2 cut(s) 29, 91
PspGI CCWGG 2 cut(s) 29, 91
PspN4I GGNNCC 1 cut(s) 159
PspPI GGNCC 1 cut(s) 157
PspPPI RGGWCCY 1 cut(s) 157
PstNI CAGNNNCTG 1 cut(s) 389
PsuI RGATCY 1 cut(s) 354
RsaI GTAC 2 cut(s) 260, 272
RsaNI GTAC 2 cut(s) 259, 271
RseI CAYNNNNRTG 1 cut(s) 198
SatI GCNGC 1 cut(s) 390
Sau3AI GATC 2 cut(s) 279, 354
Sau96I GGNCC 1 cut(s) 157
SchI GAGTC 2 cut(s) 184, 230
ScrFI CCNGG 2 cut(s) 31, 93
SduI GDGCHC 1 cut(s) 267
SetI ASST 5 cut(s) 123, 135, 153, 278, 306
SfaNI GCATC 2 cut(s) 376, 389
SinI GGWCC 1 cut(s) 157
SmiMI CAYNNNNRTG 1 cut(s) 198
Sse9I AATT 1 cut(s) 68
SsiI CCGC 2 cut(s) 5, 49
StyD4I CCNGG 2 cut(s) 29, 91
StyI CCWWGG 2 cut(s) 300, 375
TaqI TCGA 1 cut(s) 78
TasI AATT 1 cut(s) 68
TatI WGTACW 1 cut(s) 270
TseFI GTSAC 1 cut(s) 16
TseI GCWGC 1 cut(s) 389
Tsp45I GTSAC 1 cut(s) 16
TspDTI ATGAA 2 cut(s) 28, 77
TspGWI ACGGA 1 cut(s) 303
VpaK11BI GGWCC 1 cut(s) 157
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.