Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr04

Orthogroup ID

OG0006222

Physical Location & Seq

Genomic Coordinates Forward (+)

30286606 .. 30292883

Gene Structure 6278 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD04G1221700.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2133 bp

ATGTGGCTTAAGCCCGCACCATCGTGCTTCTCTATGCTAAAACCCTACTGCTCTGCAACTCCTCCACCACCACCACCGCCGACCACCGCCTTAAAAGCCCTAATGGCGAGAAAAACCACGGAGGAATCCGACGTTGGCATCTTCTGCTACATCTCTCAGCTCCCCGGATTTCGCGGCGTCTTAAAGCAAAGGTATTCGGATTTTATGGTAAACGAAGTAGATAAGGAGGGAAATGTTGTTCATTTAACCAACTTGGATGCGCCGGTAGAGGCAGTTAAGGAAAGTGGAACAACGACAAGTGATGGTGCAAGTAAAGATTATACTACCGAAATTGAATCGTTTAGAGCTCTTGCTGATCCTAATGATGCTGAGCGCTTGGAAACTCTTATCAACCAAATTAATTCCAGTAGTGATGATGGTGTTATGCCTATTGTTCTTTCCCCAGATTATGATAAATCTCATCGAACGCACTTCAAGTTCCTTGTCACCGACACAGTTGATGGACCGGATGCTTCATCAAAATGCATCCGGGTGAGAATAAATTCAGGAGGACAGAGCAACAGAGGCAGAAATTCCAGAAAAAGAAAAGAAAGAGGTGATAAACCTTTCGATAGCAGGGGTTCAGAGGATTGGTCGGAGCACGTTGGCAAGTTCCTTAGGTTTCATCTTTACAAGGAGAACAAGGATACACAGGAAGCCTTAGGAGTTATAGGAAAAATGCTTGGTGTCCAGCCAAGGTCCTTTGGGTTTGCGGGTACAAAGGATAAGCGTGCTGTCACAACTCAGAGGGTAACCGTTTTTAAGAAGCTTGCAAGTAAATTAGCTGCTCTTAATGATAGGTTGATCGGTATCAAAGTAGGTGACTTCTGTTATGTGACAGAAGGACTTGTCCTTGGCCAACTCTTGGGAAATCGGTTTACAATTACATTGAGAGGAGTTGTTGCAGATTCTGAAGATACCATCAAAGCCTCCGCAAGTGCCTTGGGAAAGCAGGGATTTATTAACTACTTTGGTTTACAACGATTTGGAAGCGGTTCTGTGCCAACTCACCGTATTGGAGCTTCCCTACTCAGAGGAGAGTGGAAATCTACCGTAAGCATGTTCCTTGATCCAAGAGAAGGGGATATCCTTTTTGTGTTTGTAGGTGTTGCACGAGAATACTATAAGGAAACTAATGACATTGAAGGGACCCTGAGGAAATTACCTCGACATCTGGTTTCTGAAAGAGCTATATTGCAGTGTCTGAAGAAATGCCCCGGAAACTACTTGCAGGCCTTGAAAGCTATTCCTAGAACTTTGAGGATGATGTATGTGCATAGTTACCAAAGCTATTTGTGGAACAATGCGGCAAGTATGAGAGTGAAGAAATATGGAACTGACCGAGTTGTGTTGGGAGACTTGGTATATTGTAAAGGAAATGAAACTGAAAAGGTGACTGAAGTTGTTACTTCTGAGTGCATAGATGAAAATGGTGATGATATGCTTGATCCTAATGATTTAGGTGATATAGCTGAAACAAATCTTCCTGAGGAAAAGCTTAATCTTGTGAAGGCTGTAACTGCAGAAGATATCCGGAGTGGAATTTATACAATTGAGGATGTCGTCCTTCCTATGCCCGGTTCAAGGGTCATTTTTCCAGAAAATGATATCGCTGATGTCTTCCATGATTTGGCAAAGAAGGATGCAATCAGCTTGACAGAGAGCGTACATAATGTCAAGGAATTCTCAATAACAAGTGTGACAGGAAATTATAGGCGGGTCTTCCAAAAACCAATGGACTTTGAATGGGAAATACTAAAATATGTTGATGGTAATGTACCTTTGGTGGATACGGACTTGGACAAAATTTCCAAAGCGAAACCTGCTAAGGTAGACAAAGAGGATCCGTCCAGTGTAAATGGAGATGGAAGTTTGCATGACAGCGCAAAACAGTCAGAGTGCATTGACAATGATATTGCGGACACCGGAGAAAAGGTGCTGGAAGTTGGATCTCTCAGCGACACTAATCCTCAGGAAACTCAGATGGCTCTCAAGTTGAGCTTTACTCTCCCAGCTTCATGTTATGCAACAATGGCAATCCGAGAGCTTCTGAAGACATCGACATCTGTTGCGTTTCACAAAACGCTAAACTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000154 GO:0000455 GO:0001522 GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005730 GO:0005732 GO:0005737 GO:0006139 GO:0006364 GO:0006396 GO:0006399 GO:0006400 GO:0006725 GO:0006807 GO:0008033 GO:0008150 GO:0008152 GO:0009451 GO:0009982 GO:0009987 GO:0010467 GO:0016070 GO:0016071 GO:0016072 GO:0016073 GO:0016556 GO:0016853 GO:0016866 GO:0022613 GO:0031118 GO:0031119 GO:0031120 GO:0031429 GO:0031974 GO:0031981 GO:0032991 GO:0034470 GO:0034641 GO:0034660 GO:0040031 GO:0042254 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043412 GO:0044085 GO:0044237 GO:0044238 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044452 GO:0044464 GO:0046483 GO:0070013 GO:0071704 GO:0071840 GO:0072588 GO:0090304 GO:1901360 GO:1902494 GO:1990481 GO:1990904

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

TruD

Protein Analysis

711

Amino Acids

78.68

Weight (kDa)

6.05

Isoelectric Point (pI)

31.19

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
TruD	PF01142	212 - 486	1.4e-66	tRNA pseudouridine synthase D (TruD)

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0006222)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G04820
fragaria_vesca	FvH4_6g03330 FvH4_6g03330 FvH4_6g03330 FvH4_6g03330 FvH4_6g03330 FvH4_6g03330 FvH4_6g03330
malus_domestica	MD04G1221700.v1.1 MD12G1237800.v1.1
prunus_persica	Prupe.6G340900_v2.0.a1
pyrus_communis	pycom04g19610 pycom04g19630 pycom12g21860 pycom12g21870
rosa_chinensis	RchiOBHm_Chr3g0451831
rosa_laevigata	RLG00000025646
rosa_multiflora	Rmu_sc0006510.1_g000014
rosa_roxburghii	Rroxscaffold_164G00436290 Rroxscaffold_6G00429000
rosa_rugosa	Rorug02G0636600 Rorug02G0636600
rosa_samantha	Rh3AG038100 Rh3BG039300 Rh3CG037800 Rh3DG038600
rosa_wichuraiana	Rw3G002840

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	1 cut(s)	1870
AccB7I	CCANNNNNTGG	2 cut(s)	904, 1669
AccI	GTMKAC	1 cut(s)	1872
AccII	CGCG	1 cut(s)	174
AccIII	TCCGGA	1 cut(s)	1572
AclWI	GGATC	6 cut(s)	350, 1103, 1481, 1877, 1890, 1996
AcoI	YGGCCR	1 cut(s)	895
AcsI	RAATTY	5 cut(s)	541, 571, 1581, 1721, 1845
AcuI	CTGAAG	4 cut(s)	972, 1265, 1458, 2111
AcyI	GRCGYC	1 cut(s)	177
AfaI	GTAC	3 cut(s)	757, 1707, 1818
AfeI	AGCGCT	1 cut(s)	374
AfiI	CCNNNNNNNGG	5 cut(s)	904, 1118, 1616, 1623, 1669
AflII	CTTAAG	1 cut(s)	8
AgsI	TTSAA	6 cut(s)	335, 475, 1184, 1279, 1623, 1784
AjuI	GAANNNNNNNTTGG	2 cut(s)	117, 149
AleI	CACNNNNGTG	1 cut(s)	22
AloI	GAACNNNNNNTCC	2 cut(s)	222, 254
Alw21I	GWGCWC	2 cut(s)	349, 642
Alw26I	GTCTC	1 cut(s)	1389
AlwI	GGATC	6 cut(s)	350, 1103, 1481, 1877, 1890, 1996
AlwNI	CAGNNNCTG	2 cut(s)	950, 1243
Aor13HI	TCCGGA	1 cut(s)	1572
Aor51HI	AGCGCT	1 cut(s)	374
AoxI	GGCC	2 cut(s)	895, 1272
ApeKI	GCWGC	1 cut(s)	824
ApoI	RAATTY	5 cut(s)	541, 571, 1581, 1721, 1845
AseI	ATTAAT	1 cut(s)	399
Asp700I	GAANNNNTTC	2 cut(s)	541, 1033
AspLEI	GCGC	3 cut(s)	262, 375, 1925
AspS9I	GGNCC	3 cut(s)	503, 738, 1188
AsuC2I	CCSGG	4 cut(s)	165, 530, 1257, 1617
AsuHPI	GGTGA	8 cut(s)	478, 544, 608, 872, 1040, 1444, 1484, 1514
AvaII	GGWCC	3 cut(s)	503, 738, 1188
AxyI	CCTNAGG	5 cut(s)	656, 700, 1193, 1527, 2010
BalI	TGGCCA	1 cut(s)	897
BamHI	GGATCC	1 cut(s)	1882
BanII	GRGCYC	1 cut(s)	349
BarI	GAAGNNNNNNTAC	2 cut(s)	1045, 1077
BauI	CACGAG	1 cut(s)	1152
BbsI	GAAGAC	3 cut(s)	1651, 1753, 2099
Bbv12I	GWGCWC	2 cut(s)	349, 642
BbvI	GCAGC	1 cut(s)	811
BccI	CCATC	8 cut(s)	28, 296, 410, 494, 968, 1802, 1898, 2017
BciVI	GTATCC	2 cut(s)	679, 1822
BcnI	CCSGG	4 cut(s)	165, 530, 1257, 1617
BcoDI	GTCTC	1 cut(s)	1389
BfaI	CTAG	2 cut(s)	1290, 2131
BfmI	CTRYAG	1 cut(s)	1560
BfoI	RGCGCY	1 cut(s)	376
BfrI	CTTAAG	1 cut(s)	8
BfuAI	ACCTGC	1 cut(s)	1870
BfuI	GTATCC	2 cut(s)	679, 1822
BglI	GCCNNNNNGGC	1 cut(s)	104
BisI	GCNGC	3 cut(s)	175, 825, 1347
BlpI	GCTNAGC	1 cut(s)	369
BlsI	GCNGC	3 cut(s)	176, 826, 1348
Bme1390I	CCNGG	4 cut(s)	165, 530, 1257, 1617
Bme18I	GGWCC	3 cut(s)	503, 738, 1188
BmgT120I	GGNCC	3 cut(s)	503, 738, 1188
BmiI	GGNNCC	3 cut(s)	1189, 1190, 1884
BmrFI	CCNGG	4 cut(s)	165, 530, 1257, 1617
BmsI	GCATC	6 cut(s)	147, 247, 355, 499, 534, 1672
BpiI	GAAGAC	3 cut(s)	1651, 1753, 2099
BplI	GAGNNNNNCTC	2 cut(s)	2029, 2061
Bpu10I	CCTNAGC	1 cut(s)	1866
Bpu1102I	GCTNAGC	1 cut(s)	369
BpuEI	CTTGAG	1 cut(s)	2015
BpuMI	CCSGG	4 cut(s)	165, 530, 1257, 1617
BsaBI	GATNNNNATC	1 cut(s)	848
BsaHI	GRCGYC	1 cut(s)	177
BsaJI	CCNNGG	6 cut(s)	117, 163, 734, 892, 981, 1255
BsaWI	WCCGGW	3 cut(s)	505, 1572, 1964
BsaXI	ACNNNNNCTCC	4 cut(s)	1386, 1416, 1959, 1989
Bsc4I	CCNNNNNNNGG	5 cut(s)	904, 1118, 1616, 1623, 1669
Bse118I	RCCGGY	1 cut(s)	262
Bse1I	ACTGG	2 cut(s)	405, 1890
Bse21I	CCTNAGG	5 cut(s)	656, 700, 1193, 1527, 2010
Bse8I	GATNNNNATC	1 cut(s)	848
BseAI	TCCGGA	1 cut(s)	1572
BseDI	CCNNGG	6 cut(s)	117, 163, 734, 892, 981, 1255
BseGI	GGATG	6 cut(s)	262, 514, 525, 1308, 1603, 1687
BseJI	GATNNNNATC	1 cut(s)	848
BseLI	CCNNNNNNNGG	5 cut(s)	904, 1118, 1616, 1623, 1669
BseNI	ACTGG	2 cut(s)	405, 1890
BseRI	GAGGAG	3 cut(s)	51, 948, 1089
BseXI	GCAGC	1 cut(s)	811
BseYI	CCCAGC	1 cut(s)	2050
Bsh1236I	CGCG	1 cut(s)	174
BshFI	GGCC	2 cut(s)	897, 1274
BsiHKAI	GWGCWC	2 cut(s)	349, 642
BsiSI	CCGG	8 cut(s)	165, 263, 506, 529, 1257, 1573, 1617, 1965
BslFI	GGGAC	1 cut(s)	1201
BslI	CCNNNNNNNGG	5 cut(s)	904, 1118, 1616, 1623, 1669
BsmAI	GTCTC	1 cut(s)	1389
BsmFI	GGGAC	1 cut(s)	1201
BsnI	GGCC	2 cut(s)	897, 1274
Bsp1286I	GDGCHC	2 cut(s)	349, 642
Bsp13I	TCCGGA	1 cut(s)	1572
Bsp143I	GATC	6 cut(s)	355, 843, 1108, 1486, 1882, 1988
Bsp1720I	GCTNAGC	1 cut(s)	369
BspANI	GGCC	2 cut(s)	897, 1274
BspEI	TCCGGA	1 cut(s)	1572
BspFNI	CGCG	1 cut(s)	174
BspLI	GGNNCC	3 cut(s)	1189, 1190, 1884
BspMAI	CTGCAG	1 cut(s)	1564
BspMI	ACCTGC	1 cut(s)	1870
BspPI	GGATC	6 cut(s)	350, 1103, 1481, 1877, 1890, 1996
BspTI	CTTAAG	1 cut(s)	8
BsrFI	RCCGGY	1 cut(s)	262
BsrI	ACTGG	2 cut(s)	405, 1890
BssAI	RCCGGY	1 cut(s)	262
BssECI	CCNNGG	6 cut(s)	117, 163, 734, 892, 981, 1255
BssMI	GATC	6 cut(s)	355, 843, 1108, 1486, 1882, 1988
BssNI	GRCGYC	1 cut(s)	177
BssSI	CACGAG	1 cut(s)	1152
BssT1I	CCWWGG	3 cut(s)	734, 892, 981
Bst2BI	CACGAG	1 cut(s)	1152
Bst4CI	ACNGT	5 cut(s)	496, 796, 1052, 1093, 1932
BstACI	GRCGYC	1 cut(s)	177
BstAFI	CTTAAG	1 cut(s)	8
BstAPI	GCANNNNNTGC	1 cut(s)	144
BstC8I	GCNNGC	4 cut(s)	15, 771, 810, 1272
BstDSI	CCRYGG	1 cut(s)	117
BstEII	GGTNACC	1 cut(s)	790
BstF5I	GGATG	6 cut(s)	262, 514, 525, 1308, 1603, 1687
BstFNI	CGCG	1 cut(s)	174
BstH2I	RGCGCY	1 cut(s)	376
BstHHI	GCGC	3 cut(s)	262, 375, 1925
BstKTI	GATC	6 cut(s)	358, 846, 1111, 1489, 1885, 1991
BstMAI	GTCTC	1 cut(s)	1389
BstMBI	GATC	6 cut(s)	355, 843, 1108, 1486, 1882, 1988
BstMWI	GCNNNNNNNGC	8 cut(s)	95, 104, 144, 564, 1280, 1559, 1862, 2072
BstNSI	RCATGY	1 cut(s)	1102
BstPI	GGTNACC	1 cut(s)	790
BstSCI	CCNGG	4 cut(s)	163, 528, 1255, 1615
BstSFI	CTRYAG	1 cut(s)	1560
BstUI	CGCG	1 cut(s)	174
BstV1I	GCAGC	1 cut(s)	811
BstV2I	GAAGAC	3 cut(s)	1651, 1753, 2099
BstX2I	RGATCY	2 cut(s)	1882, 1988
BstYI	RGATCY	2 cut(s)	1882, 1988
Bsu36I	CCTNAGG	5 cut(s)	656, 700, 1193, 1527, 2010
BsuI	GTATCC	2 cut(s)	679, 1822
BsuRI	GGCC	2 cut(s)	897, 1274
BtgI	CCRYGG	1 cut(s)	117
BtsCI	GGATG	6 cut(s)	262, 514, 525, 1308, 1603, 1687
BtsI	GCAGTG	1 cut(s)	1244
BtsIMutI	CAGTG	2 cut(s)	1244, 1897
BveI	ACCTGC	1 cut(s)	1870
Cac8I	GCNNGC	4 cut(s)	15, 771, 810, 1272
CaiI	CAGNNNCTG	2 cut(s)	950, 1243
CfoI	GCGC	3 cut(s)	262, 375, 1925
Cfr10I	RCCGGY	1 cut(s)	262
Cfr13I	GGNCC	3 cut(s)	503, 738, 1188
CseI	GACGC	1 cut(s)	166
Csp6I	GTAC	3 cut(s)	756, 1706, 1817
CviAII	CATG	4 cut(s)	1099, 1664, 1916, 2058
CviQI	GTAC	3 cut(s)	756, 1706, 1817
DpnI	GATC	6 cut(s)	357, 845, 1110, 1488, 1884, 1990
DpnII	GATC	6 cut(s)	355, 843, 1108, 1486, 1882, 1988
EaeI	YGGCCR	1 cut(s)	895
Ecl136II	GAGCTC	1 cut(s)	347
Eco130I	CCWWGG	3 cut(s)	734, 892, 981
Eco147I	AGGCCT	1 cut(s)	1274
Eco24I	GRGCYC	1 cut(s)	349
Eco32I	GATATC	3 cut(s)	1126, 1570, 1648
Eco47I	GGWCC	3 cut(s)	503, 738, 1188
Eco47III	AGCGCT	1 cut(s)	374
Eco53kI	GAGCTC	1 cut(s)	347
Eco57I	CTGAAG	4 cut(s)	972, 1265, 1458, 2111
Eco81I	CCTNAGG	5 cut(s)	656, 700, 1193, 1527, 2010
Eco91I	GGTNACC	1 cut(s)	790
EcoICRI	GAGCTC	1 cut(s)	347
EcoO109I	RGGNCCY	2 cut(s)	738, 1188
EcoO65I	GGTNACC	1 cut(s)	790
EcoRI	GAATTC	1 cut(s)	1721
EcoRV	GATATC	3 cut(s)	1126, 1570, 1648
EcoT14I	CCWWGG	3 cut(s)	734, 892, 981
EcoT22I	ATGCAT	1 cut(s)	527
EcoT38I	GRGCYC	1 cut(s)	349
ErhI	CCWWGG	3 cut(s)	734, 892, 981
FaeI	CATG	4 cut(s)	1102, 1667, 1919, 2061
FalI	AAGNNNNNCTT	2 cut(s)	2024, 2056
FaqI	GGGAC	1 cut(s)	1201
FatI	CATG	4 cut(s)	1098, 1663, 1915, 2057
FauI	CCCGC	3 cut(s)	22, 745, 1749
FblI	GTMKAC	1 cut(s)	1872
Fnu4HI	GCNGC	3 cut(s)	175, 825, 1347
FokI	GGATG	6 cut(s)	269, 512, 521, 1315, 1610, 1694
FriOI	GRGCYC	1 cut(s)	349
Fsp4HI	GCNGC	3 cut(s)	175, 825, 1347
FspBI	CTAG	2 cut(s)	1290, 2131
GlaI	GCGC	3 cut(s)	261, 374, 1924
GluI	GCNGC	3 cut(s)	175, 825, 1347
GsaI	CCCAGC	1 cut(s)	2054
HaeII	RGCGCY	1 cut(s)	376
HaeIII	GGCC	2 cut(s)	897, 1274
HapII	CCGG	8 cut(s)	165, 263, 506, 529, 1257, 1573, 1617, 1965
HgaI	GACGC	1 cut(s)	166
HhaI	GCGC	3 cut(s)	262, 375, 1925
Hin1I	GRCGYC	1 cut(s)	177
Hin1II	CATG	4 cut(s)	1102, 1667, 1919, 2061
Hin6I	GCGC	3 cut(s)	260, 373, 1923
HinP1I	GCGC	3 cut(s)	260, 373, 1923
HindIII	AAGCTT	2 cut(s)	806, 1535
HinfI	GANTC	3 cut(s)	125, 335, 947
HpaII	CCGG	8 cut(s)	165, 263, 506, 529, 1257, 1573, 1617, 1965
HphI	GGTGA	8 cut(s)	478, 544, 608, 872, 1040, 1444, 1484, 1514
Hpy166II	GTNNAC	4 cut(s)	211, 918, 1016, 1873
Hpy188III	TCNNGA	6 cut(s)	546, 576, 1526, 1573, 1637, 2012
Hpy8I	GTNNAC	4 cut(s)	211, 918, 1016, 1873
Hpy99I	CGWCG	1 cut(s)	134
HpyAV	CCTTC	6 cut(s)	875, 1112, 1178, 1543, 1616, 1672
HpyCH4III	ACNGT	5 cut(s)	496, 796, 1052, 1093, 1932
HpyCH4IV	ACGT	2 cut(s)	132, 642
HpyF10VI	GCNNNNNNNGC	8 cut(s)	95, 104, 144, 564, 1280, 1559, 1862, 2072
HpySE526I	ACGT	2 cut(s)	132, 642
Hsp92I	GRCGYC	1 cut(s)	177
Hsp92II	CATG	4 cut(s)	1102, 1667, 1919, 2061
HspAI	GCGC	3 cut(s)	260, 373, 1923
KflI	GGGWCCC	1 cut(s)	1188
Kpn2I	TCCGGA	1 cut(s)	1572
Kzo9I	GATC	6 cut(s)	355, 843, 1108, 1486, 1882, 1988
LmnI	GCTCC	3 cut(s)	165, 637, 1058
Lsp1109I	GCAGC	1 cut(s)	811
LweI	GCATC	6 cut(s)	147, 247, 355, 499, 534, 1672
MaeI	CTAG	2 cut(s)	1290, 2131
MaeII	ACGT	2 cut(s)	132, 642
MalI	GATC	6 cut(s)	357, 845, 1110, 1488, 1884, 1990
MboI	GATC	6 cut(s)	355, 843, 1108, 1486, 1882, 1988
MboII	GAAGA	9 cut(s)	133, 965, 1258, 1375, 1514, 1577, 1651, 1753, 2104
MfeI	CAATTG	1 cut(s)	1590
MflI	RGATCY	2 cut(s)	1882, 1988
MhlI	GDGCHC	2 cut(s)	349, 642
MlsI	TGGCCA	1 cut(s)	897
MluNI	TGGCCA	1 cut(s)	897
MmeI	TCCRAC	3 cut(s)	153, 615, 1966
Mox20I	TGGCCA	1 cut(s)	897
Mph1103I	ATGCAT	1 cut(s)	527
MroI	TCCGGA	1 cut(s)	1572
MroXI	GAANNNNTTC	2 cut(s)	541, 1033
MscI	TGGCCA	1 cut(s)	897
MslI	CAYNNNNRTG	3 cut(s)	22, 520, 530
Msp20I	TGGCCA	1 cut(s)	897
MspCI	CTTAAG	1 cut(s)	8
MspI	CCGG	8 cut(s)	165, 263, 506, 529, 1257, 1573, 1617, 1965
MspR9I	CCNGG	4 cut(s)	165, 530, 1257, 1617
MunI	CAATTG	1 cut(s)	1590
MvnI	CGCG	1 cut(s)	174
MwoI	GCNNNNNNNGC	8 cut(s)	95, 104, 144, 564, 1280, 1559, 1862, 2072
NciI	CCSGG	4 cut(s)	165, 530, 1257, 1617
NdeII	GATC	6 cut(s)	355, 843, 1108, 1486, 1882, 1988
NlaIII	CATG	4 cut(s)	1102, 1667, 1919, 2061
NlaIV	GGNNCC	3 cut(s)	1189, 1190, 1884
NmuCI	GTSAC	6 cut(s)	484, 775, 860, 874, 1432, 1738
NsiI	ATGCAT	1 cut(s)	527
NspI	RCATGY	1 cut(s)	1102
OliI	CACNNNNGTG	1 cut(s)	22
PceI	AGGCCT	1 cut(s)	1274
PdmI	GAANNNNTTC	2 cut(s)	541, 1033
PfeI	GAWTC	3 cut(s)	125, 335, 947
PflMI	CCANNNNNTGG	2 cut(s)	904, 1669
PkrI	GCNGC	3 cut(s)	176, 826, 1348
PpuMI	RGGWCCY	2 cut(s)	738, 1188
PshBI	ATTAAT	1 cut(s)	399
Psp124BI	GAGCTC	1 cut(s)	349
Psp5II	RGGWCCY	2 cut(s)	738, 1188
PspEI	GGTNACC	1 cut(s)	790
PspFI	CCCAGC	1 cut(s)	2050
PspN4I	GGNNCC	3 cut(s)	1189, 1190, 1884
PspPI	GGNCC	3 cut(s)	503, 738, 1188
PspPPI	RGGWCCY	2 cut(s)	738, 1188
PstI	CTGCAG	1 cut(s)	1564
PstNI	CAGNNNCTG	2 cut(s)	950, 1243
PsuI	RGATCY	2 cut(s)	1882, 1988
RsaI	GTAC	3 cut(s)	757, 1707, 1818
RsaNI	GTAC	3 cut(s)	756, 1706, 1817
RseI	CAYNNNNRTG	3 cut(s)	22, 520, 530
SacI	GAGCTC	1 cut(s)	349
SatI	GCNGC	3 cut(s)	175, 825, 1347
Sau3AI	GATC	6 cut(s)	355, 843, 1108, 1486, 1882, 1988
Sau96I	GGNCC	3 cut(s)	503, 738, 1188
ScrFI	CCNGG	4 cut(s)	165, 530, 1257, 1617
SduI	GDGCHC	2 cut(s)	349, 642
SfaNI	GCATC	6 cut(s)	147, 247, 355, 499, 534, 1672
SfcI	CTRYAG	1 cut(s)	1560
SinI	GGWCC	3 cut(s)	503, 738, 1188
SmiMI	CAYNNNNRTG	3 cut(s)	22, 520, 530
SmlI	CTYRAG	2 cut(s)	8, 2030
SmoI	CTYRAG	2 cut(s)	8, 2030
SseBI	AGGCCT	1 cut(s)	1274
SspMI	CTAG	2 cut(s)	1290, 2131
SstI	GAGCTC	1 cut(s)	349
StuI	AGGCCT	1 cut(s)	1274
StyD4I	CCNGG	4 cut(s)	163, 528, 1255, 1615
StyI	CCWWGG	3 cut(s)	734, 892, 981
TaaI	ACNGT	5 cut(s)	496, 796, 1052, 1093, 1932
TaiI	ACGT	2 cut(s)	135, 645
TaqI	TCGA	4 cut(s)	463, 609, 1207, 2099
TaqII	GACCGA	1 cut(s)	1395
TauI	GCSGC	2 cut(s)	177, 1349
TfiI	GAWTC	3 cut(s)	125, 335, 947
TscAI	CASTG	2 cut(s)	1244, 1897
TseFI	GTSAC	6 cut(s)	484, 775, 860, 874, 1432, 1738
TseI	GCWGC	1 cut(s)	824
Tsp45I	GTSAC	6 cut(s)	484, 775, 860, 874, 1432, 1738
TspDTI	ATGAA	6 cut(s)	230, 504, 653, 1434, 1479, 2046
TspGWI	ACGGA	3 cut(s)	134, 1847, 1875
TspRI	CASTG	2 cut(s)	1244, 1897
Van91I	CCANNNNNTGG	2 cut(s)	904, 1669
Vha464I	CTTAAG	1 cut(s)	8
VpaK11BI	GGWCC	3 cut(s)	503, 738, 1188
VspI	ATTAAT	1 cut(s)	399
XapI	RAATTY	5 cut(s)	541, 571, 1581, 1721, 1845
XceI	RCATGY	1 cut(s)	1102
XmiI	GTMKAC	1 cut(s)	1872
XmnI	GAANNNNTTC	2 cut(s)	541, 1033
XspI	CTAG	2 cut(s)	1290, 2131
Zsp2I	ATGCAT	1 cut(s)	527

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.