Basic Information

Type: gene

Biological Identity

Species

rosa_laevigata

Chromosome

Chr5

Orthogroup ID

OG0006222

Physical Location & Seq

Genomic Coordinates Forward (+)

47313527 .. 47320878

Gene Structure 7352 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

RLM00000025646

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2142 bp

ATGTGGCTTAACCCTGTATCGTCGTGCTTCCCGAATCTAAAACCCTACTCCTACTCCTCTGCAACTCCTCCACCACCACCAACCACCATCGTGAAAGCTCTGATGGCCATGAAGACTACGGAGGAATCCGACGTCGGAATATTCTGTTACATCTCTCAGCTCCCTGGCTTTCGTGGCATTCTTAAGCAAAGGTATTCTGATTTTATGGTGAATGAAGTCGATACCGAAGGAAATGTGGTTCATTTGACCAACTTGGAAGCTCCACCAGAGGTGGTGAGGGAAAATGGAGCGGAGACGCAGGATAGTGCAAGGAAAGATTATAGTGCTGAAATTGAAAAGTTTAGGTCTCTTGCTGGTGGTGAGGATGTTGAGCGATTGGAAGGTTTGATCAGTCAGATTAATTCAGGAAGTGAAGATGGCGTTATGCCTATTGTGCTTTCCCCGGACTATGATAAAGCTCATCGAACGGCAGTGCATAATTTTTTTAAGGAGAACTTAAAGTTCCTTGTCACCGACGCAGTGGATGGACCAGATGCTTCGTCAAAATGCATCCGTGTAAGACTAAATTCAGGAGGGCAGAATGGCAGAGGCAGGAATTCGAAAAAGCGAAAAGAAAGGGGTGATAAACCATTTGATAGCAGAGGTACTGATGACTGGTCAGATCATGTTGGCAAGTTCCTAAGGTTTCATCTTTACAAGGAGAACAAGGATACACAGGAAGCCTTAGGAATTATAGGAAAGATGCTTGGTATACAGCCAAGGGCATTTGGGTTTGCGGGTACAAAGGATAAGCGAGCTGTTTCAAGTCAAAGGGTAACCGTCTTTAAGCAGTTTGCAAGTAGATTAGCTGCTCTTAATGATAGGTTGATTGGTATCAAAGTAGGCAACTTTTGCTATGTCAAAGATGGACTTTCTCTTGGCCAACTCCTGGGAAACCGCTTTACAATTACATTGAGAGGAGTTGTTGCAGATTCTGAAGATACCATCCAGTCATCTGCAAATGCCTTGGGAAAACAGGGATTTGTTAACTACTTTGGTTTACAAAGATTTGGAAGTGGTTCAGTTCCAACACACCTTATTGGAGCTTCACTACTGAGAGGAGAGTGGAAATCTACTGCACGGAAATACTACAAGGAAACTAATGATATTGACGGGACCTTGAGGAAATTACCTCGACATCTTGTTGCTGAAATTGCTATTCTGCAGTGTCTGAAGAAATGTCCTGGAAACTACTCTCAGGCTCTGAAAGCTATTCCCAGAACTTTGAGGATGATGTATGTACATAGTTACCAGAGCTATTTGTGGAACCATGCAGCAAGTATGAGAGTGCAAAAATATGGAACTGACCGAGTTGTGGTGGGAGACTTGGTATATTGTAAAGGAAATGAAACTGAGAAGGTGACAGGAATTGTTACTTCTGAGTGTGTGGATGAAAATGTTAATGATGAAAACTGTGATGATACATTAGATCCTAGTCATTTGGATGACATATCAGAAACAAATATTCCTGAGGCGAGGGTTAATCTTGTAAAGGCTGTAACTGCAGAAGATATCGACAATGGGAATTACACGGTTGATGATATTGTCCTTCCTCTGCCAGGATCAAGGGTCATCTTTCCAGAAAATGATATTGCTGCTGTTTTTCATGATCTATCAAAGAGGGATGCGATCAGCTTGACAGAGAGCGTACATAATGTCAAGGAATTCTCAATAACGAGTGTAACTGGAAGTTACAGGCGGGTCTTCCAAAGACCAATGGACTTTGAATGGGAACTACTAAAGTATATTGATGGCAATGTACCATTGGTGGAGACAGACTTTGAAAAAATTGCTAAAACCAAGCCTGTGAATGTACACAAAGAGGACCCAACAAGTGTAAATGGAGATGGAAGTTTACATGATAGCATCAAACAGTCAGAGTGCTCTGCCAATGATATAGAACTGCAAAAAAGAGATATTGAGGGGGAAGACAAGGGAGAAAAGTTGCCACAAGTTGAATCTCTCAGTGGCACTAATCCTCAGGGAACTCAGATTGCTCTCAAGTTGAGCTTTACTCTCCCATCATCTTGTTATGCAACAATGGCCATCAGAGAGCTACTGAAGACTTCGACATCTGTTGCTTTTCACAAAACCTTGAACTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000154 GO:0000455 GO:0001522 GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005730 GO:0005732 GO:0005737 GO:0006139 GO:0006364 GO:0006396 GO:0006399 GO:0006400 GO:0006725 GO:0006807 GO:0008033 GO:0008150 GO:0008152 GO:0009451 GO:0009982 GO:0009987 GO:0010467 GO:0016070 GO:0016071 GO:0016072 GO:0016073 GO:0016556 GO:0016853 GO:0016866 GO:0022613 GO:0031118 GO:0031119 GO:0031120 GO:0031429 GO:0031974 GO:0031981 GO:0032991 GO:0034470 GO:0034641 GO:0034660 GO:0040031 GO:0042254 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043412 GO:0044085 GO:0044237 GO:0044238 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044452 GO:0044464 GO:0046483 GO:0070013 GO:0071704 GO:0071840 GO:0072588 GO:0090304 GO:1901360 GO:1902494 GO:1990481 GO:1990904

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

TruD

Protein Analysis

714

Amino Acids

79.12

Weight (kDa)

6.17

Isoelectric Point (pI)

33.26

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
TruD	PF01142	221 - 474	2.1e-59	tRNA pseudouridine synthase D (TruD)

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0006222)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G04820
fragaria_vesca	FvH4_6g03330 FvH4_6g03330 FvH4_6g03330 FvH4_6g03330 FvH4_6g03330 FvH4_6g03330 FvH4_6g03330
malus_domestica	MD04G1221700.v1.1 MD12G1237800.v1.1
prunus_persica	Prupe.6G340900_v2.0.a1
pyrus_communis	pycom04g19610 pycom04g19630 pycom12g21860 pycom12g21870
rosa_chinensis	RchiOBHm_Chr3g0451831
rosa_laevigata	RLG00000025646
rosa_multiflora	Rmu_sc0006510.1_g000014
rosa_roxburghii	Rroxscaffold_164G00436290 Rroxscaffold_6G00429000
rosa_rugosa	Rorug02G0636600 Rorug02G0636600
rosa_samantha	Rh3AG038100 Rh3BG039300 Rh3CG037800 Rh3DG038600
rosa_wichuraiana	Rw3G002840

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	1 cut(s)	135
AccB7I	CCANNNNNTGG	1 cut(s)	928
AccBSI	CCGCTC	1 cut(s)	290
AccI	GTMKAC	1 cut(s)	751
AciI	CCGC	4 cut(s)	290, 776, 937, 1738
AclWI	GGATC	2 cut(s)	1463, 1609
AcoI	YGGCCR	3 cut(s)	105, 919, 2082
AcsI	RAATTY	3 cut(s)	565, 595, 1703
AcuI	CTGAAG	3 cut(s)	996, 1232, 2120
AcyI	GRCGYC	1 cut(s)	132
AfaI	GTAC	6 cut(s)	646, 781, 1281, 1689, 1800, 1854
AfiI	CCNNNNNNNGG	3 cut(s)	928, 1354, 1598
AflII	CTTAAG	1 cut(s)	182
AgsI	TTSAA	6 cut(s)	335, 804, 1766, 1823, 1997, 2137
AjnI	CCWGG	4 cut(s)	163, 927, 1222, 1597
AleI	CACNNNNGTG	1 cut(s)	89
AloI	GAACNNNNNNTCC	2 cut(s)	222, 254
Alw21I	GWGCWC	1 cut(s)	1925
Alw26I	GTCTC	4 cut(s)	287, 351, 1356, 1805
AlwI	GGATC	2 cut(s)	1463, 1609
AlwNI	CAGNNNCTG	3 cut(s)	974, 1210, 1243
AoxI	GGCC	3 cut(s)	105, 919, 2082
ApeKI	GCWGC	3 cut(s)	848, 1313, 1634
ApoI	RAATTY	3 cut(s)	565, 595, 1703
AseI	ATTAAT	1 cut(s)	399
Asp700I	GAANNNNTTC	1 cut(s)	1057
AspS9I	GGNCC	3 cut(s)	527, 1155, 1864
AsuC2I	CCSGG	1 cut(s)	443
AsuHPI	GGTGA	6 cut(s)	220, 286, 371, 502, 632, 1411
AsuII	TTCGAA	1 cut(s)	599
AvaII	GGWCC	3 cut(s)	527, 1155, 1864
AxyI	CCTNAGG	4 cut(s)	680, 724, 1509, 2019
BalI	TGGCCA	3 cut(s)	107, 921, 2084
BbsI	GAAGAC	4 cut(s)	119, 1735, 1974, 2108
Bbv12I	GWGCWC	1 cut(s)	1925
BbvI	GCAGC	3 cut(s)	835, 1325, 1621
BceAI	ACGGC	1 cut(s)	483
BciT130I	CCWGG	4 cut(s)	165, 929, 1224, 1599
BciVI	GTATCC	1 cut(s)	703
BclI	TGATCA	1 cut(s)	387
BcnI	CCSGG	1 cut(s)	443
BcoDI	GTCTC	4 cut(s)	287, 351, 1356, 1805
BfaI	CTAG	2 cut(s)	1473, 2140
BfmI	CTRYAG	2 cut(s)	1202, 1542
BfrI	CTTAAG	1 cut(s)	182
BfuI	GTATCC	1 cut(s)	703
BisI	GCNGC	3 cut(s)	849, 1314, 1635
BlsI	GCNGC	3 cut(s)	850, 1315, 1636
Bme1390I	CCNGG	5 cut(s)	165, 443, 929, 1224, 1599
Bme18I	GGWCC	3 cut(s)	527, 1155, 1864
BmgT120I	GGNCC	3 cut(s)	527, 1155, 1864
BmiI	GGNNCC	3 cut(s)	1156, 1307, 1866
BmrFI	CCNGG	5 cut(s)	165, 443, 929, 1224, 1599
BmsI	GCATC	5 cut(s)	523, 558, 732, 1654, 1914
BpiI	GAAGAC	4 cut(s)	119, 1735, 1974, 2108
BplI	GAGNNNNNCTC	2 cut(s)	2038, 2070
Bpu14I	TTCGAA	1 cut(s)	599
BpuEI	CTTGAG	2 cut(s)	1180, 2024
BpuMI	CCSGG	1 cut(s)	443
BsaBI	GATNNNNATC	1 cut(s)	872
BsaHI	GRCGYC	1 cut(s)	132
BsaI	GGTCTC	1 cut(s)	351
BsaJI	CCNNGG	5 cut(s)	163, 441, 758, 928, 1005
BsaXI	ACNNNNNCTCC	4 cut(s)	38, 68, 1353, 1383
Bsc4I	CCNNNNNNNGG	3 cut(s)	928, 1354, 1598
Bse1I	ACTGG	3 cut(s)	659, 988, 1729
Bse21I	CCTNAGG	4 cut(s)	680, 724, 1509, 2019
Bse3DI	GCAATG	1 cut(s)	1801
Bse8I	GATNNNNATC	1 cut(s)	872
BseBI	CCWGG	4 cut(s)	165, 929, 1224, 1599
BseDI	CCNNGG	5 cut(s)	163, 441, 758, 928, 1005
BseGI	GGATG	8 cut(s)	370, 529, 549, 984, 1275, 1435, 1489, 1669
BseJI	GATNNNNATC	1 cut(s)	872
BseLI	CCNNNNNNNGG	3 cut(s)	928, 1354, 1598
BseMI	GCAATG	1 cut(s)	1801
BseMII	CTCAG	9 cut(s)	170, 1085, 1250, 1383, 1410, 1500, 2017, 2033, 2042
BseNI	ACTGG	3 cut(s)	659, 988, 1729
BseRI	GAGGAG	4 cut(s)	46, 57, 972, 1113
BseXI	GCAGC	3 cut(s)	835, 1325, 1621
BsgI	GTGCAG	1 cut(s)	1101
BshFI	GGCC	3 cut(s)	107, 921, 2084
BsiHKAI	GWGCWC	1 cut(s)	1925
BsiSI	CCGG	1 cut(s)	443
BslFI	GGGAC	1 cut(s)	1168
BslI	CCNNNNNNNGG	3 cut(s)	928, 1354, 1598
BsmAI	GTCTC	4 cut(s)	287, 351, 1356, 1805
BsmBI	CGTCTC	1 cut(s)	287
BsmFI	GGGAC	1 cut(s)	1168
BsmI	GAATGC	1 cut(s)	177
BsnI	GGCC	3 cut(s)	107, 921, 2084
Bso31I	GGTCTC	1 cut(s)	351
Bsp119I	TTCGAA	1 cut(s)	599
Bsp1286I	GDGCHC	1 cut(s)	1925
Bsp1407I	TGTACA	2 cut(s)	1279, 1852
Bsp143I	GATC	6 cut(s)	387, 661, 1468, 1601, 1648, 1668
BspACI	CCGC	4 cut(s)	290, 776, 937, 1738
BspANI	GGCC	3 cut(s)	107, 921, 2084
BspCNI	CTCAG	9 cut(s)	169, 1086, 1249, 1384, 1411, 1501, 2016, 2032, 2041
BspHI	TCATGA	1 cut(s)	1645
BspLI	GGNNCC	3 cut(s)	1156, 1307, 1866
BspMAI	CTGCAG	2 cut(s)	1206, 1546
BspPI	GGATC	2 cut(s)	1463, 1609
BspT104I	TTCGAA	1 cut(s)	599
BspTI	CTTAAG	1 cut(s)	182
BspTNI	GGTCTC	1 cut(s)	351
BsrBI	CCGCTC	1 cut(s)	290
BsrDI	GCAATG	1 cut(s)	1801
BsrGI	TGTACA	2 cut(s)	1279, 1852
BsrI	ACTGG	3 cut(s)	659, 988, 1729
BssECI	CCNNGG	5 cut(s)	163, 441, 758, 928, 1005
BssMI	GATC	6 cut(s)	387, 661, 1468, 1601, 1648, 1668
BssNAI	GTATAC	1 cut(s)	752
BssNI	GRCGYC	1 cut(s)	132
BssT1I	CCWWGG	2 cut(s)	758, 1005
Bst1107I	GTATAC	1 cut(s)	752
Bst2UI	CCWGG	4 cut(s)	165, 929, 1224, 1599
Bst4CI	ACNGT	4 cut(s)	820, 1454, 1573, 1914
BstACI	GRCGYC	1 cut(s)	132
BstAFI	CTTAAG	1 cut(s)	182
BstAPI	GCANNNNNTGC	1 cut(s)	891
BstAUI	TGTACA	2 cut(s)	1279, 1852
BstBI	TTCGAA	1 cut(s)	599
BstC8I	GCNNGC	1 cut(s)	795
BstEII	GGTNACC	1 cut(s)	814
BstENI	CCTNNNNNAGG	1 cut(s)	1596
BstF5I	GGATG	8 cut(s)	370, 529, 549, 984, 1275, 1435, 1489, 1669
BstKTI	GATC	6 cut(s)	390, 664, 1471, 1604, 1651, 1671
BstMAI	GTCTC	4 cut(s)	287, 351, 1356, 1805
BstMBI	GATC	6 cut(s)	387, 661, 1468, 1601, 1648, 1668
BstMWI	GCNNNNNNNGC	8 cut(s)	104, 174, 433, 891, 1193, 1247, 1541, 2081
BstNI	CCWGG	4 cut(s)	165, 929, 1224, 1599
BstPI	GGTNACC	1 cut(s)	814
BstSCI	CCNGG	5 cut(s)	163, 441, 927, 1222, 1597
BstSFI	CTRYAG	2 cut(s)	1202, 1542
BstV1I	GCAGC	3 cut(s)	835, 1325, 1621
BstV2I	GAAGAC	4 cut(s)	119, 1735, 1974, 2108
BstX2I	RGATCY	1 cut(s)	1468
BstYI	RGATCY	1 cut(s)	1468
BstZ17I	GTATAC	1 cut(s)	752
Bsu36I	CCTNAGG	4 cut(s)	680, 724, 1509, 2019
BsuI	GTATCC	1 cut(s)	703
BsuRI	GGCC	3 cut(s)	107, 921, 2084
BtsCI	GGATG	8 cut(s)	370, 529, 549, 984, 1275, 1435, 1489, 1669
BtsI	GCAGTG	3 cut(s)	477, 525, 1211
BtsIMutI	CAGTG	4 cut(s)	477, 525, 1211, 2011
Cac8I	GCNNGC	1 cut(s)	795
CaiI	CAGNNNCTG	3 cut(s)	974, 1210, 1243
CciI	TCATGA	1 cut(s)	1645
Cfr13I	GGNCC	3 cut(s)	527, 1155, 1864
CseI	GACGC	2 cut(s)	304, 524
Csp6I	GTAC	6 cut(s)	645, 780, 1280, 1688, 1799, 1853
CviAII	CATG	5 cut(s)	109, 665, 1310, 1646, 1898
CviQI	GTAC	6 cut(s)	645, 780, 1280, 1688, 1799, 1853
DpnI	GATC	6 cut(s)	389, 663, 1470, 1603, 1650, 1670
DpnII	GATC	6 cut(s)	387, 661, 1468, 1601, 1648, 1668
EaeI	YGGCCR	3 cut(s)	105, 919, 2082
Eco130I	CCWWGG	2 cut(s)	758, 1005
Eco31I	GGTCTC	1 cut(s)	351
Eco32I	GATATC	1 cut(s)	1552
Eco47I	GGWCC	3 cut(s)	527, 1155, 1864
Eco57I	CTGAAG	3 cut(s)	996, 1232, 2120
Eco81I	CCTNAGG	4 cut(s)	680, 724, 1509, 2019
Eco91I	GGTNACC	1 cut(s)	814
EcoNI	CCTNNNNNAGG	1 cut(s)	1596
EcoO109I	RGGNCCY	2 cut(s)	1155, 1864
EcoO65I	GGTNACC	1 cut(s)	814
EcoRI	GAATTC	2 cut(s)	595, 1703
EcoRII	CCWGG	4 cut(s)	163, 927, 1222, 1597
EcoRV	GATATC	1 cut(s)	1552
EcoT14I	CCWWGG	2 cut(s)	758, 1005
EcoT22I	ATGCAT	1 cut(s)	551
ErhI	CCWWGG	2 cut(s)	758, 1005
Esp3I	CGTCTC	1 cut(s)	287
FaeI	CATG	5 cut(s)	112, 668, 1313, 1649, 1901
FalI	AAGNNNNNCTT	6 cut(s)	479, 511, 894, 926, 2033, 2065
FaqI	GGGAC	1 cut(s)	1168
FatI	CATG	5 cut(s)	108, 664, 1309, 1645, 1897
FauI	CCCGC	2 cut(s)	769, 1731
FbaI	TGATCA	1 cut(s)	387
FblI	GTMKAC	1 cut(s)	751
Fnu4HI	GCNGC	3 cut(s)	849, 1314, 1635
FokI	GGATG	8 cut(s)	377, 536, 536, 971, 1282, 1442, 1496, 1676
Fsp4HI	GCNGC	3 cut(s)	849, 1314, 1635
FspBI	CTAG	2 cut(s)	1473, 2140
GluI	GCNGC	3 cut(s)	849, 1314, 1635
HaeIII	GGCC	3 cut(s)	107, 921, 2084
HapII	CCGG	1 cut(s)	443
HgaI	GACGC	2 cut(s)	304, 524
Hin1I	GRCGYC	1 cut(s)	132
Hin1II	CATG	5 cut(s)	112, 668, 1313, 1649, 1901
HincII	GTYRAC	1 cut(s)	1027
HindII	GTYRAC	1 cut(s)	1027
HinfI	GANTC	4 cut(s)	34, 125, 971, 1997
HpaI	GTTAAC	1 cut(s)	1027
HpaII	CCGG	1 cut(s)	443
HphI	GGTGA	6 cut(s)	220, 286, 371, 502, 632, 1411
Hpy166II	GTNNAC	5 cut(s)	752, 1027, 1040, 1855, 1895
Hpy188III	TCNNGA	7 cut(s)	31, 91, 405, 570, 1508, 1619, 1646
Hpy8I	GTNNAC	5 cut(s)	752, 1027, 1040, 1855, 1895
Hpy99I	CGWCG	4 cut(s)	25, 134, 137, 518
HpyAV	CCTTC	4 cut(s)	221, 374, 1390, 1598
HpyCH4III	ACNGT	4 cut(s)	820, 1454, 1573, 1914
HpyCH4IV	ACGT	1 cut(s)	132
HpyF10VI	GCNNNNNNNGC	8 cut(s)	104, 174, 433, 891, 1193, 1247, 1541, 2081
HpySE526I	ACGT	1 cut(s)	132
Hsp92I	GRCGYC	1 cut(s)	132
Hsp92II	CATG	5 cut(s)	112, 668, 1313, 1649, 1901
Ksp22I	TGATCA	1 cut(s)	387
KspAI	GTTAAC	1 cut(s)	1027
Kzo9I	GATC	6 cut(s)	387, 661, 1468, 1601, 1648, 1668
LmnI	GCTCC	4 cut(s)	165, 265, 287, 1082
Lsp1109I	GCAGC	3 cut(s)	835, 1325, 1621
LweI	GCATC	5 cut(s)	523, 558, 732, 1654, 1914
MaeI	CTAG	2 cut(s)	1473, 2140
MaeII	ACGT	1 cut(s)	132
MaeIII	GTNAC	9 cut(s)	146, 508, 814, 1286, 1399, 1411, 1537, 1720, 1730
MalI	GATC	6 cut(s)	389, 663, 1470, 1603, 1650, 1670
MbiI	CCGCTC	1 cut(s)	290
MboI	GATC	6 cut(s)	387, 661, 1468, 1601, 1648, 1668
MboII	GAAGA	8 cut(s)	124, 425, 989, 1225, 1559, 1735, 1979, 2113
MflI	RGATCY	1 cut(s)	1468
MhlI	GDGCHC	1 cut(s)	1925
MlsI	TGGCCA	3 cut(s)	107, 921, 2084
MluNI	TGGCCA	3 cut(s)	107, 921, 2084
MmeI	TCCRAC	3 cut(s)	115, 153, 1091
Mox20I	TGGCCA	3 cut(s)	107, 921, 2084
Mph1103I	ATGCAT	1 cut(s)	551
MroXI	GAANNNNTTC	1 cut(s)	1057
MscI	TGGCCA	3 cut(s)	107, 921, 2084
MslI	CAYNNNNRTG	2 cut(s)	89, 1482
Msp20I	TGGCCA	3 cut(s)	107, 921, 2084
MspCI	CTTAAG	1 cut(s)	182
MspI	CCGG	1 cut(s)	443
MspR9I	CCNGG	5 cut(s)	165, 443, 929, 1224, 1599
Mva1269I	GAATGC	1 cut(s)	177
MvaI	CCWGG	4 cut(s)	165, 929, 1224, 1599
MwoI	GCNNNNNNNGC	8 cut(s)	104, 174, 433, 891, 1193, 1247, 1541, 2081
NciI	CCSGG	1 cut(s)	443
NdeII	GATC	6 cut(s)	387, 661, 1468, 1601, 1648, 1668
NlaIII	CATG	5 cut(s)	112, 668, 1313, 1649, 1901
NlaIV	GGNNCC	3 cut(s)	1156, 1307, 1866
NmuCI	GTSAC	2 cut(s)	508, 1399
NsiI	ATGCAT	1 cut(s)	551
NspV	TTCGAA	1 cut(s)	599
OliI	CACNNNNGTG	1 cut(s)	89
PagI	TCATGA	1 cut(s)	1645
PcsI	WCGNNNNNNNCGW	1 cut(s)	29
PctI	GAATGC	1 cut(s)	177
PdmI	GAANNNNTTC	1 cut(s)	1057
PfeI	GAWTC	4 cut(s)	34, 125, 971, 1997
PflMI	CCANNNNNTGG	1 cut(s)	928
PfoI	TCCNGGA	1 cut(s)	1222
PkrI	GCNGC	3 cut(s)	850, 1315, 1636
PpuMI	RGGWCCY	2 cut(s)	1155, 1864
PshBI	ATTAAT	1 cut(s)	399
Psp5II	RGGWCCY	2 cut(s)	1155, 1864
Psp6I	CCWGG	4 cut(s)	163, 927, 1222, 1597
PspEI	GGTNACC	1 cut(s)	814
PspGI	CCWGG	4 cut(s)	163, 927, 1222, 1597
PspN4I	GGNNCC	3 cut(s)	1156, 1307, 1866
PspPI	GGNCC	3 cut(s)	527, 1155, 1864
PspPPI	RGGWCCY	2 cut(s)	1155, 1864
PstI	CTGCAG	2 cut(s)	1206, 1546
PstNI	CAGNNNCTG	3 cut(s)	974, 1210, 1243
PsuI	RGATCY	1 cut(s)	1468
RsaI	GTAC	6 cut(s)	646, 781, 1281, 1689, 1800, 1854
RsaNI	GTAC	6 cut(s)	645, 780, 1280, 1688, 1799, 1853
RseI	CAYNNNNRTG	2 cut(s)	89, 1482
SatI	GCNGC	3 cut(s)	849, 1314, 1635
Sau3AI	GATC	6 cut(s)	387, 661, 1468, 1601, 1648, 1668
Sau96I	GGNCC	3 cut(s)	527, 1155, 1864
ScrFI	CCNGG	5 cut(s)	165, 443, 929, 1224, 1599
SduI	GDGCHC	1 cut(s)	1925
SfaNI	GCATC	5 cut(s)	523, 558, 732, 1654, 1914
SfcI	CTRYAG	2 cut(s)	1202, 1542
SfuI	TTCGAA	1 cut(s)	599
SinI	GGWCC	3 cut(s)	527, 1155, 1864
SmiMI	CAYNNNNRTG	2 cut(s)	89, 1482
SmlI	CTYRAG	3 cut(s)	182, 1159, 2039
SmoI	CTYRAG	3 cut(s)	182, 1159, 2039
SsiI	CCGC	4 cut(s)	290, 776, 937, 1738
SspI	AATATT	2 cut(s)	141, 1504
SspMI	CTAG	2 cut(s)	1473, 2140
StyD4I	CCNGG	5 cut(s)	163, 441, 927, 1222, 1597
StyI	CCWWGG	2 cut(s)	758, 1005
TaaI	ACNGT	4 cut(s)	820, 1454, 1573, 1914
TaiI	ACGT	1 cut(s)	135
TaqI	TCGA	6 cut(s)	219, 463, 599, 1174, 1554, 2108
TaqII	GACCGA	1 cut(s)	1362
TatI	WGTACW	2 cut(s)	1279, 1852
TfiI	GAWTC	4 cut(s)	34, 125, 971, 1997
TscAI	CASTG	4 cut(s)	477, 525, 1211, 2011
TseFI	GTSAC	2 cut(s)	508, 1399
TseI	GCWGC	3 cut(s)	848, 1313, 1634
Tsp45I	GTSAC	2 cut(s)	508, 1399
TspDTI	ATGAA	8 cut(s)	125, 228, 230, 677, 1401, 1446, 1461, 1634
TspGWI	ACGGA	3 cut(s)	134, 542, 1135
TspRI	CASTG	4 cut(s)	477, 525, 1211, 2011
Van91I	CCANNNNNTGG	1 cut(s)	928
Vha464I	CTTAAG	1 cut(s)	182
VpaK11BI	GGWCC	3 cut(s)	527, 1155, 1864
VspI	ATTAAT	1 cut(s)	399
XagI	CCTNNNNNAGG	1 cut(s)	1596
XapI	RAATTY	3 cut(s)	565, 595, 1703
XmiI	GTMKAC	1 cut(s)	751
XmnI	GAANNNNTTC	1 cut(s)	1057
XspI	CTAG	2 cut(s)	1473, 2140
ZraI	GACGTC	1 cut(s)	133
Zsp2I	ATGCAT	1 cut(s)	551

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.