Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr12

Orthogroup ID

OG0006222

Physical Location & Seq

Genomic Coordinates Forward (+)

31127772 .. 31134282

Gene Structure 6511 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD12G1237800.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2043 bp

ATGTGGCTTGAGCCCGCACCATCATGCTTCTCTATGCTAAAACCCTACTGCTCTGCTACTCCTCCACCACCACCGCTAACCACCGCCTTAAAAGCCCTAGTGGCGACAACCACGGAGGAATCCGACGTCGGAATCTTCTGCTACATCTCTCAGCTCCCTGGATTTCGCGGCATTTTAAAGCAAAGGTATTCGGATTTTATGGTAAACGAAGTAGATAAGGAGGGAAATGTTGTTCATTTGACCAACTTGGATGCGCCGGTGGAGGCAGTTCAGGAAAGTGGAACAACGACAGGTGATGGTACAAGTAAAGATTATACGACCGAAATTGAATCATTTAGAGCTCTTGCTGGTCCTAATGATGCTGAGCGCTTGGAAACTTTTATCAAACAAATTAATTCCGGTGGCGAAGATGGTGTTATGCCTATTGTTCTTTCCCCAGATTATGATAAATCTCATCGAACGAGACGTAGACATGACACTTGTGCAATCAGAGAGCACTTCAAGTTCCTTGTCACTGACACAGTAGATGGACCAGATGCTTCATCAAAATGCATCCGGGTGAGGATAAATTCAGGTGGACAGAACAGCAGAGGCAGAAATTCCAGAAAACGAAAAGAAAGAGTTGATAAACCTTTTGATAGCAGGGGTTCAGAGGATTGGTCAGAGGATGTTGGCAAATTCCTTAGGTTTCATCTTTACAAGGAGAACAAGGATACACAGGAAGCCTTAGGAGTTATAGGAAAGATGCTTGGTGTCCAGCCAAGGTCCTTTGGGTTTGCGGGTACAAAGGATAAGCGTGCTGTCACAACTCAGAGGGTAACGGTTTTTAAGAAGCTTGCAAGTAAATTAGCTGCTCTTAATGATAGGTTGATCGGTATCAAAGTGGGTGACTTCTGTTATGTGACCAAAGGACTTGTCCTTGGCCAACTCTTGGGAAATCGGTTTACAATTACATTGAGAGGAGTTGTTGCAGATTCGGAAGATACCATCAAAGCCTCCGCAAGTGCCTTGGGAAAGCAGGGATTTATTAACTACTTCGGTTTACAACGATTTGGAAGCGGTTCTGTGCCAACTCACCTTATTGGAGTGTCACTACTCAGAGGAGAGTGGAAATCTACCATAAGCATGTTCCTTGATCCAAGAGAAGGGGATATCCTTTTGTGCTTCTGGGTGTTGAATGTGATTACAGAGGCACGAGAATATTATAAGGAAACTAATGATATTGAAGGGACCCTGAGGAAATTACCTCGACATCTGGTTTCTGAAAGAGCAATATTGCAGTGTCTGAAGAAATGTCCTGGAAACTACTTGCAGGCGTTGAGAGCTATTCCTAGAACTTTGAGGATGATGTATGTACATAGTTACCAAAGCTATTTGTGGAACCATGCAGCGAGTATGAGAGTGAAGAAATATGGAACTGACCAAGTTGTGTTGGGAGACTTGGTATATTGTAAAGGAAATGAGACTGAAAAAGTGACAGAAGTTGTTAGTTCTGAGTGCGTAGATGAAAATGGTGATGATACACTTGATCCTAATGATTTAGGCGATATAGCTGACACAAATCTTCCTGAGGAAAAGCTTAGTCTTGTGAAGGCTGTAACTGCAGAAGATATCCAGAGTGGAAATTATACAATTGAGGATGTTGTCCTTCCTATGCCCGGTTCAAGGGTCATTTTTCCAGAAAATGATATTGCTGATGTCTTTCATGATTTGGCAAAGAAGGATGAAATCAGCTTGACAGAGAGCGTACATGATGTCAAGGAATTCTCAATAACAAGCGTGACTGGAAATTATAGACGCATCTTCCAAAAACCAATGGACTTTGAATGGGAGATACTAAAATACAGTGATGGTACTATACCTCTGGTGGACACCGACTTGGACAAAATTGCCAAAACCAAACCCGCTAGCGTAGACAAAGAGGATCCAGCCAGTCTAAATGGAGACGGAAGTTTGCATGACAACGCAAAACAGACAGAGTGCGTTGACAATGATATCGGGGGACAACAGAGAAAAGTTGCCGGAAGTTGGATCCCTCAGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000154 GO:0000455 GO:0001522 GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005730 GO:0005732 GO:0005737 GO:0006139 GO:0006364 GO:0006396 GO:0006399 GO:0006400 GO:0006725 GO:0006807 GO:0008033 GO:0008150 GO:0008152 GO:0009451 GO:0009982 GO:0009987 GO:0010467 GO:0016070 GO:0016071 GO:0016072 GO:0016073 GO:0016556 GO:0016853 GO:0016866 GO:0022613 GO:0031118 GO:0031119 GO:0031120 GO:0031429 GO:0031974 GO:0031981 GO:0032991 GO:0034470 GO:0034641 GO:0034660 GO:0040031 GO:0042254 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043412 GO:0044085 GO:0044237 GO:0044238 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044452 GO:0044464 GO:0046483 GO:0070013 GO:0071704 GO:0071840 GO:0072588 GO:0090304 GO:1901360 GO:1902494 GO:1990481 GO:1990904

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

TruD

Protein Analysis

681

Amino Acids

75.63

Weight (kDa)

5.72

Isoelectric Point (pI)

32.29

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
TruD	PF01142	221 - 496	9.4e-67	tRNA pseudouridine synthase D (TruD)

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0006222)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G04820
fragaria_vesca	FvH4_6g03330 FvH4_6g03330 FvH4_6g03330 FvH4_6g03330 FvH4_6g03330 FvH4_6g03330 FvH4_6g03330
malus_domestica	MD04G1221700.v1.1 MD12G1237800.v1.1
prunus_persica	Prupe.6G340900_v2.0.a1
pyrus_communis	pycom04g19610 pycom04g19630 pycom12g21860 pycom12g21870
rosa_chinensis	RchiOBHm_Chr3g0451831
rosa_laevigata	RLG00000025646
rosa_multiflora	Rmu_sc0006510.1_g000014
rosa_roxburghii	Rroxscaffold_164G00436290 Rroxscaffold_6G00429000
rosa_rugosa	Rorug02G0636600 Rorug02G0636600
rosa_samantha	Rh3AG038100 Rh3BG039300 Rh3CG037800 Rh3DG038600
rosa_wichuraiana	Rw3G002840

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1206
AatII	GACGTC	1 cut(s)	129
AccB7I	CCANNNNNTGG	1 cut(s)	931
AccI	GTMKAC	2 cut(s)	469, 1914
AccII	CGCG	1 cut(s)	168
AciI	CCGC	8 cut(s)	15, 74, 84, 168, 779, 999, 1059, 1905
AclWI	GGATC	6 cut(s)	1130, 1523, 1919, 1932, 2026, 2039
AcoI	YGGCCR	1 cut(s)	922
AcsI	RAATTY	4 cut(s)	568, 598, 677, 1763
AcuI	CTGAAG	1 cut(s)	1307
AcyI	GRCGYC	1 cut(s)	126
AfaI	GTAC	5 cut(s)	301, 784, 1356, 1749, 1855
AfeI	AGCGCT	1 cut(s)	368
AfiI	CCNNNNNNNGG	5 cut(s)	931, 1145, 1658, 1665, 2028
AgsI	TTSAA	6 cut(s)	329, 502, 1177, 1226, 1665, 1826
AjnI	CCWGG	2 cut(s)	157, 1297
AloI	GAACNNNNNNTCC	2 cut(s)	216, 248
AluBI	AGCT	9 cut(s)	154, 341, 835, 851, 1325, 1371, 1553, 1579, 1734
AluI	AGCT	9 cut(s)	154, 341, 835, 851, 1325, 1371, 1553, 1579, 1734
Alw21I	GWGCWC	2 cut(s)	343, 498
Alw26I	GTCTC	4 cut(s)	457, 1431, 1457, 1938
AlwI	GGATC	6 cut(s)	1130, 1523, 1919, 1932, 2026, 2039
AlwNI	CAGNNNCTG	1 cut(s)	1285
Aor51HI	AGCGCT	1 cut(s)	368
AoxI	GGCC	1 cut(s)	922
ApeKI	GCWGC	2 cut(s)	851, 1388
ApoI	RAATTY	4 cut(s)	568, 598, 677, 1763
ArsI	GACNNNNNNTTYG	2 cut(s)	900, 932
AseI	ATTAAT	1 cut(s)	393
Asp700I	GAANNNNTTC	1 cut(s)	1060
AspLEI	GCGC	2 cut(s)	256, 369
AspS9I	GGNCC	4 cut(s)	350, 530, 765, 1230
AsuC2I	CCSGG	2 cut(s)	557, 1659
AsuHPI	GGTGA	5 cut(s)	305, 571, 899, 1067, 1526
AsuNHI	GCTAGC	1 cut(s)	1907
AvaII	GGWCC	4 cut(s)	350, 530, 765, 1230
AxyI	CCTNAGG	4 cut(s)	683, 727, 1235, 1569
BalI	TGGCCA	1 cut(s)	924
BamHI	GGATCC	2 cut(s)	1924, 2031
BanII	GRGCYC	2 cut(s)	15, 343
BauI	CACGAG	1 cut(s)	1194
Bbv12I	GWGCWC	2 cut(s)	343, 498
BbvI	GCAGC	2 cut(s)	838, 1400
BccI	CCATC	6 cut(s)	28, 290, 404, 521, 995, 1844
BciT130I	CCWGG	2 cut(s)	159, 1299
BciVI	GTATCC	1 cut(s)	706
BcnI	CCSGG	2 cut(s)	557, 1659
BcoDI	GTCTC	4 cut(s)	457, 1431, 1457, 1938
BfaI	CTAG	3 cut(s)	98, 1332, 1908
BfmI	CTRYAG	1 cut(s)	1602
BfoI	RGCGCY	1 cut(s)	370
BfuI	GTATCC	1 cut(s)	706
BglI	GCCNNNNNGGC	1 cut(s)	101
BisI	GCNGC	3 cut(s)	169, 852, 1389
BlpI	GCTNAGC	1 cut(s)	363
BlsI	GCNGC	3 cut(s)	170, 853, 1390
Bme1390I	CCNGG	4 cut(s)	159, 557, 1299, 1659
Bme18I	GGWCC	4 cut(s)	350, 530, 765, 1230
BmgT120I	GGNCC	4 cut(s)	350, 530, 765, 1230
BmiI	GGNNCC	5 cut(s)	1231, 1232, 1382, 1926, 2033
BmrFI	CCNGG	4 cut(s)	159, 557, 1299, 1659
BmsI	GCATC	6 cut(s)	241, 349, 526, 561, 735, 1809
BmtI	GCTAGC	1 cut(s)	1911
Bpu1102I	GCTNAGC	1 cut(s)	363
BpuEI	CTTGAG	1 cut(s)	29
BpuMI	CCSGG	2 cut(s)	557, 1659
BsaBI	GATNNNNATC	1 cut(s)	875
BsaHI	GRCGYC	1 cut(s)	126
BsaJI	CCNNGG	5 cut(s)	111, 157, 761, 919, 1008
BsaWI	WCCGGW	1 cut(s)	398
BsaXI	ACNNNNNCTCC	2 cut(s)	1428, 1458
Bsc4I	CCNNNNNNNGG	5 cut(s)	931, 1145, 1658, 1665, 2028
Bse118I	RCCGGY	1 cut(s)	256
Bse1I	ACTGG	2 cut(s)	1789, 1932
Bse21I	CCTNAGG	4 cut(s)	683, 727, 1235, 1569
Bse8I	GATNNNNATC	1 cut(s)	875
BseBI	CCWGG	2 cut(s)	159, 1299
BseDI	CCNNGG	5 cut(s)	111, 157, 761, 919, 1008
BseGI	GGATG	6 cut(s)	256, 552, 673, 1350, 1645, 1729
BseJI	GATNNNNATC	1 cut(s)	875
BseLI	CCNNNNNNNGG	5 cut(s)	931, 1145, 1658, 1665, 2028
BseMII	CTCAG	7 cut(s)	164, 354, 824, 1111, 1226, 1485, 1560
BseNI	ACTGG	2 cut(s)	1789, 1932
BseRI	GAGGAG	3 cut(s)	51, 975, 1116
BseXI	GCAGC	2 cut(s)	838, 1400
Bsh1236I	CGCG	1 cut(s)	168
Bsh1285I	CGRYCG	1 cut(s)	321
BshFI	GGCC	1 cut(s)	924
BsiEI	CGRYCG	1 cut(s)	321
BsiHKAI	GWGCWC	2 cut(s)	343, 498
BsiSI	CCGG	5 cut(s)	257, 399, 556, 1659, 2022
BslFI	GGGAC	2 cut(s)	1243, 2016
BslI	CCNNNNNNNGG	5 cut(s)	931, 1145, 1658, 1665, 2028
BsmAI	GTCTC	4 cut(s)	457, 1431, 1457, 1938
BsmBI	CGTCTC	2 cut(s)	457, 1938
BsmFI	GGGAC	2 cut(s)	1243, 2016
BsnI	GGCC	1 cut(s)	924
Bsp1286I	GDGCHC	3 cut(s)	15, 343, 498
Bsp1407I	TGTACA	1 cut(s)	1354
Bsp143I	GATC	5 cut(s)	870, 1135, 1528, 1924, 2031
Bsp1720I	GCTNAGC	1 cut(s)	363
BspACI	CCGC	8 cut(s)	15, 74, 84, 168, 779, 999, 1059, 1905
BspANI	GGCC	1 cut(s)	924
BspCNI	CTCAG	7 cut(s)	163, 355, 823, 1110, 1227, 1486, 1561
BspFNI	CGCG	1 cut(s)	168
BspHI	TCATGA	1 cut(s)	1705
BspLI	GGNNCC	5 cut(s)	1231, 1232, 1382, 1926, 2033
BspMAI	CTGCAG	1 cut(s)	1606
BspOI	GCTAGC	1 cut(s)	1911
BspPI	GGATC	6 cut(s)	1130, 1523, 1919, 1932, 2026, 2039
BsrFI	RCCGGY	1 cut(s)	256
BsrGI	TGTACA	1 cut(s)	1354
BsrI	ACTGG	2 cut(s)	1789, 1932
BssAI	RCCGGY	1 cut(s)	256
BssECI	CCNNGG	5 cut(s)	111, 157, 761, 919, 1008
BssMI	GATC	5 cut(s)	870, 1135, 1528, 1924, 2031
BssNI	GRCGYC	1 cut(s)	126
BssSI	CACGAG	1 cut(s)	1194
BssT1I	CCWWGG	3 cut(s)	761, 919, 1008
Bst2BI	CACGAG	1 cut(s)	1194
Bst2UI	CCWGG	2 cut(s)	159, 1299
Bst4CI	ACNGT	3 cut(s)	523, 823, 1847
BstACI	GRCGYC	1 cut(s)	126
BstAUI	TGTACA	1 cut(s)	1354
BstC8I	GCNNGC	5 cut(s)	15, 798, 837, 1314, 1909
BstDSI	CCRYGG	1 cut(s)	111
BstF5I	GGATG	6 cut(s)	256, 552, 673, 1350, 1645, 1729
BstFNI	CGCG	1 cut(s)	168
BstH2I	RGCGCY	1 cut(s)	370
BstHHI	GCGC	2 cut(s)	256, 369
BstKTI	GATC	5 cut(s)	873, 1138, 1531, 1927, 2034
BstMAI	GTCTC	4 cut(s)	457, 1431, 1457, 1938
BstMBI	GATC	5 cut(s)	870, 1135, 1528, 1924, 2031
BstMCI	CGRYCG	1 cut(s)	321
BstMWI	GCNNNNNNNGC	4 cut(s)	92, 101, 1322, 1601
BstNI	CCWGG	2 cut(s)	159, 1299
BstNSI	RCATGY	1 cut(s)	1129
BstSCI	CCNGG	4 cut(s)	157, 555, 1297, 1657
BstSFI	CTRYAG	1 cut(s)	1602
BstUI	CGCG	1 cut(s)	168
BstV1I	GCAGC	2 cut(s)	838, 1400
BstX2I	RGATCY	2 cut(s)	1924, 2031
BstYI	RGATCY	2 cut(s)	1924, 2031
Bsu36I	CCTNAGG	4 cut(s)	683, 727, 1235, 1569
BsuI	GTATCC	1 cut(s)	706
BsuRI	GGCC	1 cut(s)	924
BtgI	CCRYGG	1 cut(s)	111
BtsCI	GGATG	6 cut(s)	256, 552, 673, 1350, 1645, 1729
BtsI	GCAGTG	1 cut(s)	1286
BtsIMutI	CAGTG	3 cut(s)	513, 1286, 1852
Cac8I	GCNNGC	5 cut(s)	15, 798, 837, 1314, 1909
CaiI	CAGNNNCTG	1 cut(s)	1285
CciI	TCATGA	1 cut(s)	1705
CfoI	GCGC	2 cut(s)	256, 369
Cfr10I	RCCGGY	1 cut(s)	256
Cfr13I	GGNCC	4 cut(s)	350, 530, 765, 1230
CseI	GACGC	1 cut(s)	1806
Csp6I	GTAC	5 cut(s)	300, 783, 1355, 1748, 1854
CviAII	CATG	7 cut(s)	24, 473, 1126, 1385, 1706, 1751, 1958
CviQI	GTAC	5 cut(s)	300, 783, 1355, 1748, 1854
DpnI	GATC	5 cut(s)	872, 1137, 1530, 1926, 2033
DpnII	GATC	5 cut(s)	870, 1135, 1528, 1924, 2031
DraI	TTTAAA	1 cut(s)	177
EaeI	YGGCCR	1 cut(s)	922
Ecl136II	GAGCTC	1 cut(s)	341
Eco130I	CCWWGG	3 cut(s)	761, 919, 1008
Eco24I	GRGCYC	2 cut(s)	15, 343
Eco32I	GATATC	3 cut(s)	1153, 1612, 1996
Eco47I	GGWCC	4 cut(s)	350, 530, 765, 1230
Eco47III	AGCGCT	1 cut(s)	368
Eco53kI	GAGCTC	1 cut(s)	341
Eco57I	CTGAAG	1 cut(s)	1307
Eco81I	CCTNAGG	4 cut(s)	683, 727, 1235, 1569
EcoICRI	GAGCTC	1 cut(s)	341
EcoO109I	RGGNCCY	2 cut(s)	765, 1230
EcoRI	GAATTC	1 cut(s)	1763
EcoRII	CCWGG	2 cut(s)	157, 1297
EcoRV	GATATC	3 cut(s)	1153, 1612, 1996
EcoT14I	CCWWGG	3 cut(s)	761, 919, 1008
EcoT22I	ATGCAT	1 cut(s)	554
EcoT38I	GRGCYC	2 cut(s)	15, 343
ErhI	CCWWGG	3 cut(s)	761, 919, 1008
Esp3I	CGTCTC	2 cut(s)	457, 1938
FaeI	CATG	7 cut(s)	27, 476, 1129, 1388, 1709, 1754, 1961
FaqI	GGGAC	2 cut(s)	1243, 2016
FatI	CATG	7 cut(s)	23, 472, 1125, 1384, 1705, 1750, 1957
FauI	CCCGC	3 cut(s)	22, 772, 1912
FblI	GTMKAC	2 cut(s)	469, 1914
Fnu4HI	GCNGC	3 cut(s)	169, 852, 1389
FokI	GGATG	6 cut(s)	263, 539, 680, 1357, 1652, 1736
FriOI	GRGCYC	2 cut(s)	15, 343
Fsp4HI	GCNGC	3 cut(s)	169, 852, 1389
FspBI	CTAG	3 cut(s)	98, 1332, 1908
GlaI	GCGC	2 cut(s)	255, 368
GluI	GCNGC	3 cut(s)	169, 852, 1389
HaeII	RGCGCY	1 cut(s)	370
HaeIII	GGCC	1 cut(s)	924
HapII	CCGG	5 cut(s)	257, 399, 556, 1659, 2022
HgaI	GACGC	1 cut(s)	1806
HhaI	GCGC	2 cut(s)	256, 369
Hin1I	GRCGYC	1 cut(s)	126
Hin1II	CATG	7 cut(s)	27, 476, 1129, 1388, 1709, 1754, 1961
Hin6I	GCGC	2 cut(s)	254, 367
HinP1I	GCGC	2 cut(s)	254, 367
HincII	GTYRAC	1 cut(s)	1987
HindII	GTYRAC	1 cut(s)	1987
HindIII	AAGCTT	2 cut(s)	833, 1577
HinfI	GANTC	4 cut(s)	119, 132, 329, 974
HpaII	CCGG	5 cut(s)	257, 399, 556, 1659, 2022
HphI	GGTGA	5 cut(s)	305, 571, 899, 1067, 1526
Hpy166II	GTNNAC	8 cut(s)	205, 470, 578, 945, 1043, 1870, 1915, 1987
Hpy188III	TCNNGA	6 cut(s)	272, 603, 1568, 1615, 1679, 1706
Hpy8I	GTNNAC	8 cut(s)	205, 470, 578, 945, 1043, 1870, 1915, 1987
Hpy99I	CGWCG	2 cut(s)	128, 131
HpyAV	CCTTC	5 cut(s)	1139, 1220, 1585, 1658, 1714
HpyCH4III	ACNGT	3 cut(s)	523, 823, 1847
HpyCH4IV	ACGT	2 cut(s)	126, 466
HpyCH4V	TGCA	9 cut(s)	485, 552, 839, 971, 1279, 1312, 1388, 1604, 1957
HpyF10VI	GCNNNNNNNGC	4 cut(s)	92, 101, 1322, 1601
HpySE526I	ACGT	2 cut(s)	126, 466
Hsp92I	GRCGYC	1 cut(s)	126
Hsp92II	CATG	7 cut(s)	27, 476, 1129, 1388, 1709, 1754, 1961
HspAI	GCGC	2 cut(s)	254, 367
KflI	GGGWCCC	1 cut(s)	1230
Kzo9I	GATC	5 cut(s)	870, 1135, 1528, 1924, 2031
LmnI	GCTCC	1 cut(s)	159
Lsp1109I	GCAGC	2 cut(s)	838, 1400
LweI	GCATC	6 cut(s)	241, 349, 526, 561, 735, 1809
MaeI	CTAG	3 cut(s)	98, 1332, 1908
MaeII	ACGT	2 cut(s)	126, 466
MalI	GATC	5 cut(s)	872, 1137, 1530, 1926, 2033
MboI	GATC	5 cut(s)	870, 1135, 1528, 1924, 2031
MboII	GAAGA	8 cut(s)	127, 419, 992, 1300, 1417, 1556, 1619, 1795
MfeI	CAATTG	1 cut(s)	1632
MflI	RGATCY	2 cut(s)	1924, 2031
MhlI	GDGCHC	3 cut(s)	15, 343, 498
MlsI	TGGCCA	1 cut(s)	924
MluNI	TGGCCA	1 cut(s)	924
MmeI	TCCRAC	3 cut(s)	109, 147, 2009
Mox20I	TGGCCA	1 cut(s)	924
Mph1103I	ATGCAT	1 cut(s)	554
MroXI	GAANNNNTTC	1 cut(s)	1060
MscI	TGGCCA	1 cut(s)	924
MseI	TTAA	6 cut(s)	89, 176, 393, 828, 858, 1029
MslI	CAYNNNNRTG	4 cut(s)	22, 547, 557, 1124
Msp20I	TGGCCA	1 cut(s)	924
MspI	CCGG	5 cut(s)	257, 399, 556, 1659, 2022
MspR9I	CCNGG	4 cut(s)	159, 557, 1299, 1659
MunI	CAATTG	1 cut(s)	1632
MvaI	CCWGG	2 cut(s)	159, 1299
MvnI	CGCG	1 cut(s)	168
MwoI	GCNNNNNNNGC	4 cut(s)	92, 101, 1322, 1601
NciI	CCSGG	2 cut(s)	557, 1659
NdeII	GATC	5 cut(s)	870, 1135, 1528, 1924, 2031
NheI	GCTAGC	1 cut(s)	1907
NlaIII	CATG	7 cut(s)	27, 476, 1129, 1388, 1709, 1754, 1961
NlaIV	GGNNCC	5 cut(s)	1231, 1232, 1382, 1926, 2033
NmuCI	GTSAC	7 cut(s)	511, 802, 887, 901, 1089, 1474, 1780
NsiI	ATGCAT	1 cut(s)	554
NspI	RCATGY	1 cut(s)	1129
PagI	TCATGA	1 cut(s)	1705
PcsI	WCGNNNNNNNCGW	1 cut(s)	463
PdmI	GAANNNNTTC	1 cut(s)	1060
PfeI	GAWTC	4 cut(s)	119, 132, 329, 974
PflMI	CCANNNNNTGG	1 cut(s)	931
PfoI	TCCNGGA	1 cut(s)	1297
PkrI	GCNGC	3 cut(s)	170, 853, 1390
PpuMI	RGGWCCY	2 cut(s)	765, 1230
PshBI	ATTAAT	1 cut(s)	393
PsiI	TTATAA	1 cut(s)	1206
Psp124BI	GAGCTC	1 cut(s)	343
Psp5II	RGGWCCY	2 cut(s)	765, 1230
Psp6I	CCWGG	2 cut(s)	157, 1297
PspGI	CCWGG	2 cut(s)	157, 1297
PspN4I	GGNNCC	5 cut(s)	1231, 1232, 1382, 1926, 2033
PspPI	GGNCC	4 cut(s)	350, 530, 765, 1230
PspPPI	RGGWCCY	2 cut(s)	765, 1230
PstI	CTGCAG	1 cut(s)	1606
PstNI	CAGNNNCTG	1 cut(s)	1285
PsuI	RGATCY	2 cut(s)	1924, 2031
RsaI	GTAC	5 cut(s)	301, 784, 1356, 1749, 1855
RsaNI	GTAC	5 cut(s)	300, 783, 1355, 1748, 1854
RseI	CAYNNNNRTG	4 cut(s)	22, 547, 557, 1124
SacI	GAGCTC	1 cut(s)	343
SaqAI	TTAA	6 cut(s)	89, 176, 393, 828, 858, 1029
SatI	GCNGC	3 cut(s)	169, 852, 1389
Sau3AI	GATC	5 cut(s)	870, 1135, 1528, 1924, 2031
Sau96I	GGNCC	4 cut(s)	350, 530, 765, 1230
ScrFI	CCNGG	4 cut(s)	159, 557, 1299, 1659
SduI	GDGCHC	3 cut(s)	15, 343, 498
SfaNI	GCATC	6 cut(s)	241, 349, 526, 561, 735, 1809
SfcI	CTRYAG	1 cut(s)	1602
SgrAI	CRCCGGYG	1 cut(s)	256
SinI	GGWCC	4 cut(s)	350, 530, 765, 1230
SmiMI	CAYNNNNRTG	4 cut(s)	22, 547, 557, 1124
SmlI	CTYRAG	1 cut(s)	8
SmoI	CTYRAG	1 cut(s)	8
SsiI	CCGC	8 cut(s)	15, 74, 84, 168, 779, 999, 1059, 1905
SspI	AATATT	2 cut(s)	1202, 1275
SspMI	CTAG	3 cut(s)	98, 1332, 1908
SstI	GAGCTC	1 cut(s)	343
StyD4I	CCNGG	4 cut(s)	157, 555, 1297, 1657
StyI	CCWWGG	3 cut(s)	761, 919, 1008
TaaI	ACNGT	3 cut(s)	523, 823, 1847
TaiI	ACGT	2 cut(s)	129, 469
TaqI	TCGA	2 cut(s)	457, 1249
TaqII	GACCGA	1 cut(s)	335
TatI	WGTACW	1 cut(s)	1354
TauI	GCSGC	1 cut(s)	171
TfiI	GAWTC	4 cut(s)	119, 132, 329, 974
Tru1I	TTAA	6 cut(s)	89, 176, 393, 828, 858, 1029
Tru9I	TTAA	6 cut(s)	89, 176, 393, 828, 858, 1029
TscAI	CASTG	3 cut(s)	520, 1286, 1852
TseFI	GTSAC	7 cut(s)	511, 802, 887, 901, 1089, 1474, 1780
TseI	GCWGC	2 cut(s)	851, 1388
Tsp45I	GTSAC	7 cut(s)	511, 802, 887, 901, 1089, 1474, 1780
TspDTI	ATGAA	6 cut(s)	224, 531, 680, 1521, 1694, 1740
TspGWI	ACGGA	2 cut(s)	128, 1962
TspRI	CASTG	3 cut(s)	520, 1286, 1852
Van91I	CCANNNNNTGG	1 cut(s)	931
VpaK11BI	GGWCC	4 cut(s)	350, 530, 765, 1230
VspI	ATTAAT	1 cut(s)	393
XapI	RAATTY	4 cut(s)	568, 598, 677, 1763
XceI	RCATGY	1 cut(s)	1129
XmiI	GTMKAC	2 cut(s)	469, 1914
XmnI	GAANNNNTTC	1 cut(s)	1060
XspI	CTAG	3 cut(s)	98, 1332, 1908
ZraI	GACGTC	1 cut(s)	127
Zsp2I	ATGCAT	1 cut(s)	554

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.