Basic Information

Type: gene

Biological Identity

Species

rosa_roxburghii

Chromosome

GWHEROQ00000006

Orthogroup ID

OG0006222

Physical Location & Seq

Genomic Coordinates Reverse (-)

49370155 .. 49378145

Gene Structure 7991 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rroxscaffold_6G00429000.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1665 bp

ATGTGGCTTAACCCTGTATCGTCGTGCTTCCCGAATCTAAAACCCTACTCCTACTCCCCTGCAACTCCTCCACCACCACCAACCACCACCGTGAAAGCTCTGATGGCCATGAAGACTACGGAGGAATCCGACGTCGGAATATTCTGTTACATCTCTCAGCTCCCTGGCTTTCGCGGCATTCTTAAGCAAAGGTATTCTGATTTTATGGTGAATGAAGTCGATACCGAAGGAAATGTGGTTCATTTGACCAACTTGGAAGCTCCACCAGAGGTAGTGAAGGAAAATGGAGCGAAGACGCAGGATGCTGCAAGTAAAGATTATAGTGCTGAAATCGAAAAGTTTAGGTCTCTTGCTGGTGGTGAGGATGTTGAGCGATTGGAAGCTTTGATCAGCCAGATTAATTCTGGAAGTGAAGATGGTGTTATGCCTATTGTTCTTTCGCCAGACTATGATAAAGCTCATCGAACGGCAGTGCATAATTTTTTTAAGGAGAACTTAAAGTTCCTTGTCACCGATGCAGTGGATGGACCGGATGCTTCGTCAAAATGCATCCGTGTAAGACTAAATTCAGGAGGGCAGAATGGCAGAGGCAGGAATTCGAAAAAGCGAAAAGAAAGGGGTGATAAACCATTTGATAGCAGAGGTACTGATGACTGGTCAGAGCATGTTGCCAAGTTCCTAAGTTCTTCTTCAGCTTACTCAAAGATTCTTCCAATGTCAATGTTCAGGTTTCATCTTTACAAGGAGAACAAGGATACACAGGAAGCTTTAGGAATTATAGGAAAGATGCTTGGAATACAGCCAAGGGCATTTGGGTTTGCGGGTACAAAGGATAAGCGTGCTGTTTCAAGTCAAAGGGTAAGAACTGACCGAGTTGTGGTGGGAGACTTGGTATATTGTAAAGGAAATGAAACTGAGAAGGTGACAGGAATTGTTAGTTCTGAGTGTGTGGATGAAAATGTTAATGTTGAAAACTGTGATGATACATTTGATCCTAGTCATTTGGATGACATATCAGAAACAAATATTCCTGAGGCGAGGGTTAATGTTGTAAAGGCTGTAACTGCAGAAGATATCGACAACGGGAATTACACGATTGATGATATTGTCCTTCCTCTGCCAGGATCAAGAGTCATCCTTCCAGAAAATGATATTGCTGCTGTTTTTCATGATCTAGCAAAGAAGGATGCGATCAGCTTGACAGAGAGCGTGCATAATGTCAAGGAATTCTCAATAACGAATGTGACTGGAAGTTACAGGCGGGTCTTCCAAAAACCAATGGACTTTGAATGGGAATTACTTAAGTATATTGATGGCAATATACCATTGGTGGAGACAGACTTTGAAAAAATTGACAAAACCAAGCCTGTGAATGTACACAAAGAGGACCCAACAAGTGTAAATGGAGATGGAAGTTTACATGATAGCATCAAACAGTCAGAGTGCTCTGCCAATGATATAGAACTGCAGAAAAGAGATATTGAGGTGGAAGACAAGGGAGAAAAATTGCCACAAGTTGAATCTCTCGGTGGCACTAATCCTCAGGGAACTCAGATTGCTCTGAAGTTGAGCTTTACTCTCCCATCATCTTGTTATGCAACAATGGCCATCAGAGAGCTACTGAAGACTTCGACATCTGTTGCTTTTCACAAAACCTTGAACTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000154 GO:0000455 GO:0001522 GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005730 GO:0005732 GO:0005737 GO:0006139 GO:0006364 GO:0006396 GO:0006399 GO:0006400 GO:0006725 GO:0006807 GO:0008033 GO:0008150 GO:0008152 GO:0009451 GO:0009982 GO:0009987 GO:0010467 GO:0016070 GO:0016071 GO:0016072 GO:0016073 GO:0016556 GO:0016853 GO:0016866 GO:0022613 GO:0031118 GO:0031119 GO:0031120 GO:0031429 GO:0031974 GO:0031981 GO:0032991 GO:0034470 GO:0034641 GO:0034660 GO:0040031 GO:0042254 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043412 GO:0044085 GO:0044237 GO:0044238 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044452 GO:0044464 GO:0046483 GO:0070013 GO:0071704 GO:0071840 GO:0072588 GO:0090304 GO:1901360 GO:1902494 GO:1990481 GO:1990904

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

TruD

Protein Analysis

554

Amino Acids

61.29

Weight (kDa)

5.22

Isoelectric Point (pI)

38.27

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
TruD	PF01142	244 - 289	3.6e-08	tRNA pseudouridine synthase D (TruD)

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0006222)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G04820
fragaria_vesca	FvH4_6g03330 FvH4_6g03330 FvH4_6g03330 FvH4_6g03330 FvH4_6g03330 FvH4_6g03330 FvH4_6g03330
malus_domestica	MD04G1221700.v1.1 MD12G1237800.v1.1
prunus_persica	Prupe.6G340900_v2.0.a1
pyrus_communis	pycom04g19610 pycom04g19630 pycom12g21860 pycom12g21870
rosa_chinensis	RchiOBHm_Chr3g0451831
rosa_laevigata	RLG00000025646
rosa_multiflora	Rmu_sc0006510.1_g000014
rosa_roxburghii	Rroxscaffold_164G00436290 Rroxscaffold_6G00429000
rosa_rugosa	Rorug02G0636600 Rorug02G0636600
rosa_samantha	Rh3AG038100 Rh3BG039300 Rh3CG037800 Rh3DG038600
rosa_wichuraiana	Rw3G002840

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	1 cut(s)	135
AccII	CGCG	1 cut(s)	174
AciI	CCGC	3 cut(s)	174, 821, 1261
AclWI	GGATC	2 cut(s)	986, 1132
AcoI	YGGCCR	2 cut(s)	105, 1605
AcsI	RAATTY	3 cut(s)	565, 595, 1226
AcuI	CTGAAG	3 cut(s)	675, 1583, 1643
AcyI	GRCGYC	1 cut(s)	132
AfaI	GTAC	3 cut(s)	646, 826, 1377
AfiI	CCNNNNNNNGG	2 cut(s)	877, 1121
AflII	CTTAAG	2 cut(s)	182, 1301
AgsI	TTSAA	6 cut(s)	849, 971, 1289, 1346, 1520, 1660
AjnI	CCWGG	2 cut(s)	163, 1120
AleI	CACNNNNGTG	1 cut(s)	89
AloI	GAACNNNNNNTCC	2 cut(s)	222, 254
Alw21I	GWGCWC	1 cut(s)	1448
Alw26I	GTCTC	3 cut(s)	351, 879, 1328
AlwI	GGATC	2 cut(s)	986, 1132
AoxI	GGCC	2 cut(s)	105, 1605
ApeKI	GCWGC	2 cut(s)	305, 1157
ApoI	RAATTY	3 cut(s)	565, 595, 1226
AseI	ATTAAT	1 cut(s)	399
AspS9I	GGNCC	2 cut(s)	527, 1387
AsuHPI	GGTGA	5 cut(s)	220, 371, 502, 632, 934
AsuII	TTCGAA	1 cut(s)	599
AvaII	GGWCC	2 cut(s)	527, 1387
AxyI	CCTNAGG	2 cut(s)	1032, 1542
BalI	TGGCCA	2 cut(s)	107, 1607
BbsI	GAAGAC	5 cut(s)	119, 299, 1258, 1497, 1631
Bbv12I	GWGCWC	1 cut(s)	1448
BbvI	GCAGC	2 cut(s)	292, 1144
BccI	CCATC	7 cut(s)	97, 410, 518, 1307, 1403, 1591, 1616
BceAI	ACGGC	1 cut(s)	483
BciT130I	CCWGG	2 cut(s)	165, 1122
BciVI	GTATCC	1 cut(s)	748
BclI	TGATCA	1 cut(s)	387
BcoDI	GTCTC	3 cut(s)	351, 879, 1328
BfaI	CTAG	3 cut(s)	996, 1175, 1663
BfmI	CTRYAG	2 cut(s)	1065, 1466
BfrI	CTTAAG	2 cut(s)	182, 1301
BfuI	GTATCC	1 cut(s)	748
BisI	GCNGC	3 cut(s)	175, 306, 1158
BlsI	GCNGC	3 cut(s)	176, 307, 1159
Bme1390I	CCNGG	2 cut(s)	165, 1122
Bme18I	GGWCC	2 cut(s)	527, 1387
BmgT120I	GGNCC	2 cut(s)	527, 1387
BmiI	GGNNCC	1 cut(s)	1389
BmrFI	CCNGG	2 cut(s)	165, 1122
BmsI	GCATC	7 cut(s)	292, 505, 523, 558, 777, 1177, 1437
BpiI	GAAGAC	5 cut(s)	119, 299, 1258, 1497, 1631
BplI	GAGNNNNNCTC	2 cut(s)	1561, 1593
Bpu14I	TTCGAA	1 cut(s)	599
BsaHI	GRCGYC	1 cut(s)	132
BsaI	GGTCTC	1 cut(s)	351
BsaJI	CCNNGG	2 cut(s)	163, 803
BsaWI	WCCGGW	1 cut(s)	529
BsaXI	ACNNNNNCTCC	4 cut(s)	38, 68, 876, 906
Bsc4I	CCNNNNNNNGG	2 cut(s)	877, 1121
Bse1I	ACTGG	2 cut(s)	659, 1252
Bse21I	CCTNAGG	2 cut(s)	1032, 1542
BseBI	CCWGG	2 cut(s)	165, 1122
BseDI	CCNNGG	2 cut(s)	163, 803
BseGI	GGATG	9 cut(s)	307, 370, 529, 538, 549, 958, 1012, 1134, 1192
BseLI	CCNNNNNNNGG	2 cut(s)	877, 1121
BseMII	CTCAG	6 cut(s)	170, 906, 933, 1023, 1556, 1565
BseNI	ACTGG	2 cut(s)	659, 1252
BseRI	GAGGAG	1 cut(s)	57
BseXI	GCAGC	2 cut(s)	292, 1144
Bsh1236I	CGCG	1 cut(s)	174
BshFI	GGCC	2 cut(s)	107, 1607
BsiHKAI	GWGCWC	1 cut(s)	1448
BsiSI	CCGG	1 cut(s)	530
BslI	CCNNNNNNNGG	2 cut(s)	877, 1121
BsmAI	GTCTC	3 cut(s)	351, 879, 1328
BsmI	GAATGC	1 cut(s)	177
BsnI	GGCC	2 cut(s)	107, 1607
Bso31I	GGTCTC	1 cut(s)	351
Bsp119I	TTCGAA	1 cut(s)	599
Bsp1286I	GDGCHC	1 cut(s)	1448
Bsp1407I	TGTACA	1 cut(s)	1375
Bsp143I	GATC	5 cut(s)	387, 991, 1124, 1171, 1191
BspACI	CCGC	3 cut(s)	174, 821, 1261
BspANI	GGCC	2 cut(s)	107, 1607
BspCNI	CTCAG	6 cut(s)	169, 907, 934, 1024, 1555, 1564
BspFNI	CGCG	1 cut(s)	174
BspHI	TCATGA	1 cut(s)	1168
BspLI	GGNNCC	1 cut(s)	1389
BspMAI	CTGCAG	2 cut(s)	1069, 1470
BspPI	GGATC	2 cut(s)	986, 1132
BspT104I	TTCGAA	1 cut(s)	599
BspTI	CTTAAG	2 cut(s)	182, 1301
BspTNI	GGTCTC	1 cut(s)	351
BsrGI	TGTACA	1 cut(s)	1375
BsrI	ACTGG	2 cut(s)	659, 1252
BssECI	CCNNGG	2 cut(s)	163, 803
BssMI	GATC	5 cut(s)	387, 991, 1124, 1171, 1191
BssNI	GRCGYC	1 cut(s)	132
BssT1I	CCWWGG	1 cut(s)	803
Bst2UI	CCWGG	2 cut(s)	165, 1122
Bst4CI	ACNGT	3 cut(s)	91, 977, 1437
BstACI	GRCGYC	1 cut(s)	132
BstAFI	CTTAAG	2 cut(s)	182, 1301
BstAUI	TGTACA	1 cut(s)	1375
BstBI	TTCGAA	1 cut(s)	599
BstC8I	GCNNGC	2 cut(s)	840, 1211
BstDEI	CTNAG	7 cut(s)	156, 680, 915, 942, 1032, 1542, 1551
BstENI	CCTNNNNNAGG	1 cut(s)	1119
BstF5I	GGATG	9 cut(s)	307, 370, 529, 538, 549, 958, 1012, 1134, 1192
BstFNI	CGCG	1 cut(s)	174
BstKTI	GATC	5 cut(s)	390, 994, 1127, 1174, 1194
BstMAI	GTCTC	3 cut(s)	351, 879, 1328
BstMBI	GATC	5 cut(s)	387, 991, 1124, 1171, 1191
BstMWI	GCNNNNNNNGC	4 cut(s)	104, 174, 1064, 1604
BstNI	CCWGG	2 cut(s)	165, 1122
BstNSI	RCATGY	1 cut(s)	668
BstSCI	CCNGG	2 cut(s)	163, 1120
BstSFI	CTRYAG	2 cut(s)	1065, 1466
BstUI	CGCG	1 cut(s)	174
BstV1I	GCAGC	2 cut(s)	292, 1144
BstV2I	GAAGAC	5 cut(s)	119, 299, 1258, 1497, 1631
Bsu36I	CCTNAGG	2 cut(s)	1032, 1542
BsuI	GTATCC	1 cut(s)	748
BsuRI	GGCC	2 cut(s)	107, 1607
BtsCI	GGATG	9 cut(s)	307, 370, 529, 538, 549, 958, 1012, 1134, 1192
BtsI	GCAGTG	2 cut(s)	477, 525
BtsIMutI	CAGTG	2 cut(s)	477, 525
Cac8I	GCNNGC	2 cut(s)	840, 1211
CciI	TCATGA	1 cut(s)	1168
Cfr13I	GGNCC	2 cut(s)	527, 1387
CseI	GACGC	1 cut(s)	304
Csp6I	GTAC	3 cut(s)	645, 825, 1376
CviAII	CATG	4 cut(s)	109, 665, 1169, 1421
CviQI	GTAC	3 cut(s)	645, 825, 1376
DdeI	CTNAG	7 cut(s)	156, 680, 915, 942, 1032, 1542, 1551
DpnI	GATC	5 cut(s)	389, 993, 1126, 1173, 1193
DpnII	GATC	5 cut(s)	387, 991, 1124, 1171, 1191
EaeI	YGGCCR	2 cut(s)	105, 1605
Eco130I	CCWWGG	1 cut(s)	803
Eco31I	GGTCTC	1 cut(s)	351
Eco32I	GATATC	1 cut(s)	1075
Eco47I	GGWCC	2 cut(s)	527, 1387
Eco57I	CTGAAG	3 cut(s)	675, 1583, 1643
Eco81I	CCTNAGG	2 cut(s)	1032, 1542
EcoNI	CCTNNNNNAGG	1 cut(s)	1119
EcoO109I	RGGNCCY	1 cut(s)	1387
EcoRI	GAATTC	2 cut(s)	595, 1226
EcoRII	CCWGG	2 cut(s)	163, 1120
EcoRV	GATATC	1 cut(s)	1075
EcoT14I	CCWWGG	1 cut(s)	803
EcoT22I	ATGCAT	1 cut(s)	551
ErhI	CCWWGG	1 cut(s)	803
FaeI	CATG	4 cut(s)	112, 668, 1172, 1424
FalI	AAGNNNNNCTT	6 cut(s)	479, 511, 673, 705, 1556, 1588
FatI	CATG	4 cut(s)	108, 664, 1168, 1420
FauI	CCCGC	2 cut(s)	814, 1254
FbaI	TGATCA	1 cut(s)	387
Fnu4HI	GCNGC	3 cut(s)	175, 306, 1158
FokI	GGATG	9 cut(s)	314, 377, 536, 536, 545, 965, 1019, 1121, 1199
Fsp4HI	GCNGC	3 cut(s)	175, 306, 1158
FspBI	CTAG	3 cut(s)	996, 1175, 1663
GluI	GCNGC	3 cut(s)	175, 306, 1158
HaeIII	GGCC	2 cut(s)	107, 1607
HapII	CCGG	1 cut(s)	530
HgaI	GACGC	1 cut(s)	304
Hin1I	GRCGYC	1 cut(s)	132
Hin1II	CATG	4 cut(s)	112, 668, 1172, 1424
HindIII	AAGCTT	2 cut(s)	381, 765
HinfI	GANTC	5 cut(s)	34, 125, 706, 1131, 1520
HpaII	CCGG	1 cut(s)	530
HphI	GGTGA	5 cut(s)	220, 371, 502, 632, 934
Hpy166II	GTNNAC	2 cut(s)	1378, 1418
Hpy188III	TCNNGA	7 cut(s)	31, 405, 570, 1031, 1128, 1142, 1169
Hpy8I	GTNNAC	2 cut(s)	1378, 1418
Hpy99I	CGWCG	3 cut(s)	25, 134, 137
HpyAV	CCTTC	6 cut(s)	221, 271, 913, 1121, 1148, 1177
HpyCH4III	ACNGT	3 cut(s)	91, 977, 1437
HpyCH4IV	ACGT	1 cut(s)	132
HpyCH4V	TGCA	9 cut(s)	62, 308, 475, 518, 549, 1067, 1213, 1468, 1598
HpyF10VI	GCNNNNNNNGC	4 cut(s)	104, 174, 1064, 1604
HpyF3I	CTNAG	7 cut(s)	156, 680, 915, 942, 1032, 1542, 1551
HpySE526I	ACGT	1 cut(s)	132
Hsp92I	GRCGYC	1 cut(s)	132
Hsp92II	CATG	4 cut(s)	112, 668, 1172, 1424
Ksp22I	TGATCA	1 cut(s)	387
Kzo9I	GATC	5 cut(s)	387, 991, 1124, 1171, 1191
LmnI	GCTCC	3 cut(s)	165, 265, 287
Lsp1109I	GCAGC	2 cut(s)	292, 1144
LweI	GCATC	7 cut(s)	292, 505, 523, 558, 777, 1177, 1437
MaeI	CTAG	3 cut(s)	996, 1175, 1663
MaeII	ACGT	1 cut(s)	132
MaeIII	GTNAC	6 cut(s)	146, 508, 922, 1060, 1243, 1253
MalI	GATC	5 cut(s)	389, 993, 1126, 1173, 1193
MboI	GATC	5 cut(s)	387, 991, 1124, 1171, 1191
MhlI	GDGCHC	1 cut(s)	1448
MlsI	TGGCCA	2 cut(s)	107, 1607
MluNI	TGGCCA	2 cut(s)	107, 1607
MlyI	GAGTC	1 cut(s)	1140
MmeI	TCCRAC	2 cut(s)	115, 153
Mox20I	TGGCCA	2 cut(s)	107, 1607
Mph1103I	ATGCAT	1 cut(s)	551
MscI	TGGCCA	2 cut(s)	107, 1607
MseI	TTAA	8 cut(s)	9, 183, 399, 486, 497, 963, 1044, 1302
MslI	CAYNNNNRTG	2 cut(s)	89, 1005
Msp20I	TGGCCA	2 cut(s)	107, 1607
MspCI	CTTAAG	2 cut(s)	182, 1301
MspI	CCGG	1 cut(s)	530
MspR9I	CCNGG	2 cut(s)	165, 1122
Mva1269I	GAATGC	1 cut(s)	177
MvaI	CCWGG	2 cut(s)	165, 1122
MvnI	CGCG	1 cut(s)	174
MwoI	GCNNNNNNNGC	4 cut(s)	104, 174, 1064, 1604
NdeII	GATC	5 cut(s)	387, 991, 1124, 1171, 1191
NlaIII	CATG	4 cut(s)	112, 668, 1172, 1424
NlaIV	GGNNCC	1 cut(s)	1389
NmuCI	GTSAC	3 cut(s)	508, 922, 1243
NsiI	ATGCAT	1 cut(s)	551
NspI	RCATGY	1 cut(s)	668
NspV	TTCGAA	1 cut(s)	599
OliI	CACNNNNGTG	1 cut(s)	89
PagI	TCATGA	1 cut(s)	1168
PcsI	WCGNNNNNNNCGW	1 cut(s)	29
PctI	GAATGC	1 cut(s)	177
PfeI	GAWTC	4 cut(s)	34, 125, 706, 1520
PkrI	GCNGC	3 cut(s)	176, 307, 1159
PleI	GAGTC	1 cut(s)	1139
PpsI	GAGTC	1 cut(s)	1139
PpuMI	RGGWCCY	1 cut(s)	1387
PshBI	ATTAAT	1 cut(s)	399
Psp5II	RGGWCCY	1 cut(s)	1387
Psp6I	CCWGG	2 cut(s)	163, 1120
PspGI	CCWGG	2 cut(s)	163, 1120
PspN4I	GGNNCC	1 cut(s)	1389
PspPI	GGNCC	2 cut(s)	527, 1387
PspPPI	RGGWCCY	1 cut(s)	1387
PstI	CTGCAG	2 cut(s)	1069, 1470
RsaI	GTAC	3 cut(s)	646, 826, 1377
RsaNI	GTAC	3 cut(s)	645, 825, 1376
RseI	CAYNNNNRTG	2 cut(s)	89, 1005
SaqAI	TTAA	8 cut(s)	9, 183, 399, 486, 497, 963, 1044, 1302
SatI	GCNGC	3 cut(s)	175, 306, 1158
Sau3AI	GATC	5 cut(s)	387, 991, 1124, 1171, 1191
Sau96I	GGNCC	2 cut(s)	527, 1387
SchI	GAGTC	1 cut(s)	1140
ScrFI	CCNGG	2 cut(s)	165, 1122
SduI	GDGCHC	1 cut(s)	1448
SfaNI	GCATC	7 cut(s)	292, 505, 523, 558, 777, 1177, 1437
SfcI	CTRYAG	2 cut(s)	1065, 1466
SfuI	TTCGAA	1 cut(s)	599
SinI	GGWCC	2 cut(s)	527, 1387
SmiMI	CAYNNNNRTG	2 cut(s)	89, 1005
SmlI	CTYRAG	2 cut(s)	182, 1301
SmoI	CTYRAG	2 cut(s)	182, 1301
SsiI	CCGC	3 cut(s)	174, 821, 1261
SspI	AATATT	2 cut(s)	141, 1027
SspMI	CTAG	3 cut(s)	996, 1175, 1663
StyD4I	CCNGG	2 cut(s)	163, 1120
StyI	CCWWGG	1 cut(s)	803
TaaI	ACNGT	3 cut(s)	91, 977, 1437
TaiI	ACGT	1 cut(s)	135
TaqI	TCGA	6 cut(s)	219, 333, 463, 599, 1077, 1631
TaqII	GACCGA	1 cut(s)	885
TatI	WGTACW	1 cut(s)	1375
TauI	GCSGC	1 cut(s)	177
TfiI	GAWTC	4 cut(s)	34, 125, 706, 1520
Tru1I	TTAA	8 cut(s)	9, 183, 399, 486, 497, 963, 1044, 1302
Tru9I	TTAA	8 cut(s)	9, 183, 399, 486, 497, 963, 1044, 1302
TscAI	CASTG	2 cut(s)	477, 525
TseFI	GTSAC	3 cut(s)	508, 922, 1243
TseI	GCWGC	2 cut(s)	305, 1157
Tsp45I	GTSAC	3 cut(s)	508, 922, 1243
TspDTI	ATGAA	7 cut(s)	125, 228, 230, 722, 924, 969, 1157
TspGWI	ACGGA	2 cut(s)	134, 542
TspRI	CASTG	2 cut(s)	477, 525
Vha464I	CTTAAG	2 cut(s)	182, 1301
VpaK11BI	GGWCC	2 cut(s)	527, 1387
VspI	ATTAAT	1 cut(s)	399
XagI	CCTNNNNNAGG	1 cut(s)	1119
XapI	RAATTY	3 cut(s)	565, 595, 1226
XceI	RCATGY	1 cut(s)	668
XcmI	CCANNNNNNNNNTGG	1 cut(s)	401
XspI	CTAG	3 cut(s)	996, 1175, 1663
ZraI	GACGTC	1 cut(s)	133
Zsp2I	ATGCAT	1 cut(s)	551

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.