MD04G1236700.v1.1

Small ubiquitin-related modifier

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr04
OG0011425
Physical Location & Seq
Forward (+)
31540065 .. 31543810
3746 bp
Loading structure...
UTR
Exon/CDS
Intron
MD04G1236700.v1.1.491

Sequence Viewer

Length: 351 bp
ATGTTACAGAACACTTGTTTGAACTTTGTGGGAATGGACGATCACGTACCAGCAATCACCAGTGAACCGCTCGTCAGGCTTTCTCCTGAGAAAGACGGGGATCCTATGATTCTTCTCAAGGTGCAGAGCCAACAGGGTTCTGTCATCTCCTACATGACCGATCTCCATATGCATCTTAAACATGTAACTCGAGATTACTGCAGACGTCTAAATGTGAGCTTTGAAGTGACACGTTTCCTCTATGATGGAAGAAGGGTCCAACAAACCGATACCCCTGCAAAGCTCGAGATGGAGGAGGATGATGCTATTGATGCAATGGTGGATATGATTGGTGGCGGAGGAGGCTATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03013 ko05418 map03013 map05418
Pfam Domains
Protein Families
Rad60-SLD

Protein Analysis

117

Amino Acids

13.1

Weight (kDa)

4.87

Isoelectric Point (pI)

56.4

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rad60-SLD PF11976 37 - 107 5.4e-13 Ubiquitin-2 like Rad60 SUMO-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 208
AccBSI CCGCTC 1 cut(s) 70
AciI CCGC 2 cut(s) 68, 336
AclWI GGATC 2 cut(s) 95, 108
AcyI GRCGYC 1 cut(s) 205
AfaI GTAC 1 cut(s) 48
AflIII ACRYGT 2 cut(s) 181, 230
AgsI TTSAA 2 cut(s) 22, 224
AluBI AGCT 2 cut(s) 219, 283
AluI AGCT 2 cut(s) 219, 283
AlwI GGATC 2 cut(s) 95, 108
Ama87I CYCGRG 2 cut(s) 189, 284
AspS9I GGNCC 1 cut(s) 256
AsuHPI GGTGA 1 cut(s) 49
AvaI CYCGRG 2 cut(s) 189, 284
AvaII GGWCC 1 cut(s) 256
BamHI GGATCC 1 cut(s) 100
BccI CCATC 2 cut(s) 239, 283
BcgI CGANNNNNNTGC 4 cut(s) 180, 214, 257, 291
BfmI CTRYAG 1 cut(s) 199
Bme18I GGWCC 1 cut(s) 256
BmeT110I CYCGRG 2 cut(s) 189, 284
BmgT120I GGNCC 1 cut(s) 256
BmiI GGNNCC 2 cut(s) 102, 257
BmsI GCATC 3 cut(s) 181, 292, 301
BpuEI CTTGAG 1 cut(s) 101
BsaAI YACGTR 1 cut(s) 46
BsaHI GRCGYC 1 cut(s) 205
Bse1I ACTGG 1 cut(s) 60
Bse3DI GCAATG 1 cut(s) 321
BseGI GGATG 1 cut(s) 304
BseMI GCAATG 1 cut(s) 321
BseMII CTCAG 1 cut(s) 78
BseNI ACTGG 1 cut(s) 60
BseRI GAGGAG 1 cut(s) 308
BsgI GTGCAG 1 cut(s) 143
BsiHKCI CYCGRG 2 cut(s) 189, 284
BsoBI CYCGRG 2 cut(s) 189, 284
Bsp143I GATC 3 cut(s) 40, 100, 160
BspACI CCGC 2 cut(s) 68, 336
BspCNI CTCAG 1 cut(s) 79
BspLI GGNNCC 2 cut(s) 102, 257
BspMAI CTGCAG 1 cut(s) 203
BspPI GGATC 2 cut(s) 95, 108
BsrBI CCGCTC 1 cut(s) 70
BsrDI GCAATG 1 cut(s) 321
BsrI ACTGG 1 cut(s) 60
BssMI GATC 3 cut(s) 40, 100, 160
BssNI GRCGYC 1 cut(s) 205
BstACI GRCGYC 1 cut(s) 205
BstBAI YACGTR 1 cut(s) 46
BstDEI CTNAG 1 cut(s) 87
BstF5I GGATG 1 cut(s) 304
BstKTI GATC 3 cut(s) 43, 103, 163
BstMBI GATC 3 cut(s) 40, 100, 160
BstMWI GCNNNNNNNGC 3 cut(s) 76, 311, 342
BstNSI RCATGY 1 cut(s) 185
BstSFI CTRYAG 1 cut(s) 199
BstX2I RGATCY 1 cut(s) 100
BstYI RGATCY 1 cut(s) 100
BtsCI GGATG 1 cut(s) 304
BtsIMutI CAGTG 1 cut(s) 67
Cfr13I GGNCC 1 cut(s) 256
Csp6I GTAC 1 cut(s) 47
CviAII CATG 2 cut(s) 154, 182
CviJI RGCY 5 cut(s) 79, 129, 219, 283, 345
CviKI_1 RGCY 5 cut(s) 79, 129, 219, 283, 345
CviQI GTAC 1 cut(s) 47
DdeI CTNAG 1 cut(s) 87
DpnI GATC 3 cut(s) 42, 102, 162
DpnII GATC 3 cut(s) 40, 100, 160
EciI GGCGGA 1 cut(s) 351
Eco47I GGWCC 1 cut(s) 256
Eco88I CYCGRG 2 cut(s) 189, 284
EcoT22I ATGCAT 1 cut(s) 174
FaeI CATG 2 cut(s) 157, 185
FaiI YATR 7 cut(s) 107, 155, 168, 170, 183, 243, 326
FatI CATG 2 cut(s) 153, 181
FauNDI CATATG 1 cut(s) 168
FokI GGATG 1 cut(s) 311
Hin1I GRCGYC 1 cut(s) 205
Hin1II CATG 2 cut(s) 157, 185
HinfI GANTC 1 cut(s) 109
HphI GGTGA 1 cut(s) 49
Hpy166II GTNNAC 1 cut(s) 65
Hpy188III TCNNGA 3 cut(s) 86, 191, 286
Hpy8I GTNNAC 1 cut(s) 65
HpyAV CCTTC 1 cut(s) 246
HpyCH4IV ACGT 3 cut(s) 45, 205, 232
HpyCH4V TGCA 5 cut(s) 124, 172, 201, 278, 314
HpyF10VI GCNNNNNNNGC 3 cut(s) 76, 311, 342
HpyF3I CTNAG 1 cut(s) 87
HpySE526I ACGT 3 cut(s) 45, 205, 232
Hsp92I GRCGYC 1 cut(s) 205
Hsp92II CATG 2 cut(s) 157, 185
Kzo9I GATC 3 cut(s) 40, 100, 160
LpnPI CCDG 6 cut(s) 61, 63, 73, 99, 119, 288
LweI GCATC 3 cut(s) 181, 292, 301
MaeII ACGT 3 cut(s) 45, 205, 232
MaeIII GTNAC 3 cut(s) 3, 184, 226
MalI GATC 3 cut(s) 42, 102, 162
MbiI CCGCTC 1 cut(s) 70
MboI GATC 3 cut(s) 40, 100, 160
MboII GAAGA 2 cut(s) 104, 261
MflI RGATCY 1 cut(s) 100
MmeI TCCRAC 1 cut(s) 283
MnlI CCTC 5 cut(s) 248, 286, 289, 332, 335
Mph1103I ATGCAT 1 cut(s) 174
MseI TTAA 2 cut(s) 177, 349
MwoI GCNNNNNNNGC 3 cut(s) 76, 311, 342
NdeI CATATG 1 cut(s) 168
NdeII GATC 3 cut(s) 40, 100, 160
NlaIII CATG 2 cut(s) 157, 185
NlaIV GGNNCC 2 cut(s) 102, 257
NmuCI GTSAC 1 cut(s) 226
NsiI ATGCAT 1 cut(s) 174
NspI RCATGY 1 cut(s) 185
PaeR7I CTCGAG 2 cut(s) 189, 284
PciI ACATGT 1 cut(s) 181
PfeI GAWTC 1 cut(s) 109
Ppu21I YACGTR 1 cut(s) 46
PscI ACATGT 1 cut(s) 181
PspN4I GGNNCC 2 cut(s) 102, 257
PspPI GGNCC 1 cut(s) 256
PstI CTGCAG 1 cut(s) 203
PsuI RGATCY 1 cut(s) 100
RsaI GTAC 1 cut(s) 48
RsaNI GTAC 1 cut(s) 47
SaqAI TTAA 2 cut(s) 177, 349
Sau3AI GATC 3 cut(s) 40, 100, 160
Sau96I GGNCC 1 cut(s) 256
SetI ASST 6 cut(s) 48, 123, 208, 221, 235, 285
SfaNI GCATC 3 cut(s) 181, 292, 301
SfcI CTRYAG 1 cut(s) 199
Sfr274I CTCGAG 2 cut(s) 189, 284
SinI GGWCC 1 cut(s) 256
SlaI CTCGAG 2 cut(s) 189, 284
SmlI CTYRAG 3 cut(s) 116, 189, 284
SmoI CTYRAG 3 cut(s) 116, 189, 284
SsiI CCGC 2 cut(s) 68, 336
TaiI ACGT 3 cut(s) 48, 208, 235
TaqI TCGA 2 cut(s) 190, 285
TaqII GACCGA 1 cut(s) 173
TfiI GAWTC 1 cut(s) 109
Tru1I TTAA 2 cut(s) 177, 349
Tru9I TTAA 2 cut(s) 177, 349
TscAI CASTG 1 cut(s) 67
TseFI GTSAC 1 cut(s) 226
Tsp45I GTSAC 1 cut(s) 226
TspRI CASTG 1 cut(s) 67
VpaK11BI GGWCC 1 cut(s) 256
XceI RCATGY 1 cut(s) 185
XhoI CTCGAG 2 cut(s) 189, 284
ZraI GACGTC 1 cut(s) 206
Zsp2I ATGCAT 1 cut(s) 174
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.