Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr04

Orthogroup ID

OG0006466

Physical Location & Seq

Genomic Coordinates Forward (+)

32133686 .. 32135724

Gene Structure 2039 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD04G1246200.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 561 bp

ATGGCTCTGCAGTCAGCCGCACTCTCTCCTCGCTCCGTCGGCGCATCCACATCGCTACCCTGCGCCGCCAAGTACCCGATCATTTGCTCCTCCTCTGCTTCGAGTTCGAGCTGCTTCCTTTCGCAGAGGAAGAATGCGGCTGCGGTTGGAATTGGATTGAGTAAGAGGAAGATGGGCTCCTGGGGGTGTACGGTAAGGTCGAACTTGGACACTGTCGGTCCCACTGTGAATGTGGGCCAAGTCACGGAGGTCGACAAGGACACCTTCTGGCCCATAGTCAAGGCCGCCGGCGACAAGACCGTCGTCCTCGACATGTATACCCAATGGTGTGGACCTTGCAAAGTGATAGCTCCAAAATTTCAAGAATTGTCCAAGAAGTATCTTGATGTCGTATTTTTAAAGCTTGATTGTAGCCAAGAAAATAAGCCATTGGCGAAGGAGCTTGGGATAAGGGTGGTGCCAACTTTCAAGATTTTGAAGGACAACAAGGTTGTAAAAGAGGTGAAGGGGGCCAAATTCGACGATTTAGTTGTTGCTATAGACGCTGTTAGATCGAGCTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004791 GO:0004857 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0006109 GO:0006950 GO:0006979 GO:0008047 GO:0008150 GO:0008152 GO:0009314 GO:0009416 GO:0009507 GO:0009532 GO:0009534 GO:0009536 GO:0009570 GO:0009579 GO:0009628 GO:0009636 GO:0009642 GO:0009987 GO:0015035 GO:0015036 GO:0016209 GO:0016491 GO:0016651 GO:0016667 GO:0016668 GO:0016671 GO:0019222 GO:0019725 GO:0019904 GO:0030234 GO:0031976 GO:0031984 GO:0033554 GO:0034599 GO:0042221 GO:0042592 GO:0043085 GO:0043086 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044092 GO:0044093 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0045454 GO:0047134 GO:0050789 GO:0050790 GO:0050794 GO:0050896 GO:0051716 GO:0055114 GO:0065007 GO:0065008 GO:0065009 GO:0070887 GO:0080090 GO:0097237 GO:0098754 GO:0098772 GO:0098869 GO:1990748

KEGG Pathways

Metabolic & Signaling

ko04621 ko05418 map04621 map05418

Pfam Domains

Protein Families

Thioredoxin

Protein Analysis

187

Amino Acids

19.96

Weight (kDa)

9.35

Isoelectric Point (pI)

37.37

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Thioredoxin	PF00085	81 - 180	3.4e-23	Thioredoxin

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Tree File

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Publication-ready

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Orthologous Genes (Group: OG0006466)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G02730 AT5G16400
fragaria_vesca	FvH4_6g00380 FvH4_6g00380
malus_domestica	MD04G1246200.v1.1 MD12G1263900.v1.1
prunus_persica	Prupe.6G362500_v2.0.a1 Prupe.6G362500_v2.0.a1
pyrus_communis	pycom12g23990 pycom15g06310
rosa_chinensis	RchiOBHm_Chr3g0447721
rosa_laevigata	RLG00000025978
rosa_multiflora	Rmu_sc0003550.1_g000001 Rmu_ssc0000391.1_g000003 Rmu_ssc0000391.1_g000009
rosa_roxburghii	Rroxscaffold_6G00425920
rosa_rugosa	Rorug02G0604800 Rorug02G0604900 Rorug02G0605000
rosa_samantha	Rh3AG004600 Rh3BG004100 Rh3BG004300 Rh3CG004100 Rh3CG004600 Rh3DG004500
rosa_wichuraiana	Rw3G000340

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	299
AccB1I	GGYRCC	1 cut(s)	457
AccI	GTMKAC	2 cut(s)	252, 317
AciI	CCGC	5 cut(s)	18, 66, 137, 143, 285
AcsI	RAATTY	2 cut(s)	356, 515
AfaI	GTAC	2 cut(s)	74, 190
AfiI	CCNNNNNNNGG	1 cut(s)	244
AflIII	ACRYGT	1 cut(s)	312
AgsI	TTSAA	3 cut(s)	362, 469, 478
AjnI	CCWGG	1 cut(s)	179
AluBI	AGCT	5 cut(s)	111, 350, 403, 442, 558
AluI	AGCT	5 cut(s)	111, 350, 403, 442, 558
AoxI	GGCC	4 cut(s)	235, 269, 282, 510
ApeKI	GCWGC	2 cut(s)	111, 140
ApoI	RAATTY	2 cut(s)	356, 515
AspLEI	GCGC	2 cut(s)	44, 65
AspS9I	GGNCC	5 cut(s)	218, 235, 270, 332, 510
AsuHPI	GGTGA	1 cut(s)	514
AvaII	GGWCC	2 cut(s)	218, 332
BanI	GGYRCC	1 cut(s)	457
BanII	GRGCYC	1 cut(s)	179
BbvI	GCAGC	2 cut(s)	98, 127
BccI	CCATC	1 cut(s)	166
BcgI	CGANNNNNNTGC	2 cut(s)	33, 67
BciT130I	CCWGG	1 cut(s)	181
BfmI	CTRYAG	2 cut(s)	8, 537
BisI	GCNGC	6 cut(s)	18, 66, 112, 138, 141, 285
BlsI	GCNGC	6 cut(s)	19, 67, 113, 139, 142, 286
Bme1390I	CCNGG	1 cut(s)	181
Bme18I	GGWCC	2 cut(s)	218, 332
BmgT120I	GGNCC	5 cut(s)	218, 235, 270, 332, 510
BmiI	GGNNCC	4 cut(s)	178, 220, 459, 511
BmrFI	CCNGG	1 cut(s)	181
BmsI	GCATC	1 cut(s)	53
BoxI	GACNNNNGTC	1 cut(s)	302
BsaJI	CCNNGG	1 cut(s)	180
Bsc4I	CCNNNNNNNGG	1 cut(s)	244
Bse118I	RCCGGY	1 cut(s)	287
BseBI	CCWGG	1 cut(s)	181
BseDI	CCNNGG	1 cut(s)	180
BseGI	GGATG	1 cut(s)	44
BseLI	CCNNNNNNNGG	1 cut(s)	244
BseRI	GAGGAG	3 cut(s)	18, 79, 82
BseXI	GCAGC	2 cut(s)	98, 127
BshFI	GGCC	4 cut(s)	237, 271, 284, 512
BshNI	GGYRCC	1 cut(s)	457
BsiSI	CCGG	1 cut(s)	288
BslFI	GGGAC	1 cut(s)	204
BslI	CCNNNNNNNGG	1 cut(s)	244
BsmFI	GGGAC	1 cut(s)	204
BsmI	GAATGC	1 cut(s)	139
BsnI	GGCC	4 cut(s)	237, 271, 284, 512
Bsp1286I	GDGCHC	1 cut(s)	179
Bsp143I	GATC	2 cut(s)	78, 551
BspACI	CCGC	5 cut(s)	18, 66, 137, 143, 285
BspANI	GGCC	4 cut(s)	237, 271, 284, 512
BspLI	GGNNCC	4 cut(s)	178, 220, 459, 511
BspMAI	CTGCAG	1 cut(s)	12
BspT107I	GGYRCC	1 cut(s)	457
BsrFI	RCCGGY	1 cut(s)	287
BssAI	RCCGGY	1 cut(s)	287
BssECI	CCNNGG	1 cut(s)	180
BssMI	GATC	2 cut(s)	78, 551
BssNAI	GTATAC	1 cut(s)	318
Bst1107I	GTATAC	1 cut(s)	318
Bst2UI	CCWGG	1 cut(s)	181
Bst4CI	ACNGT	4 cut(s)	193, 214, 226, 301
BstC8I	GCNNGC	1 cut(s)	289
BstF5I	GGATG	1 cut(s)	44
BstHHI	GCGC	2 cut(s)	44, 65
BstKTI	GATC	2 cut(s)	81, 554
BstMBI	GATC	2 cut(s)	78, 551
BstMWI	GCNNNNNNNGC	2 cut(s)	39, 542
BstNI	CCWGG	1 cut(s)	181
BstNSI	RCATGY	1 cut(s)	316
BstPAI	GACNNNNGTC	1 cut(s)	302
BstSCI	CCNGG	1 cut(s)	179
BstSFI	CTRYAG	2 cut(s)	8, 537
BstV1I	GCAGC	2 cut(s)	98, 127
BstXI	CCANNNNNNTGG	1 cut(s)	329
BstZ17I	GTATAC	1 cut(s)	318
BsuRI	GGCC	4 cut(s)	237, 271, 284, 512
BtgZI	GCGATG	1 cut(s)	36
BtsCI	GGATG	1 cut(s)	44
BtsIMutI	CAGTG	2 cut(s)	210, 222
Cac8I	GCNNGC	1 cut(s)	289
CfoI	GCGC	2 cut(s)	44, 65
Cfr10I	RCCGGY	1 cut(s)	287
Cfr13I	GGNCC	5 cut(s)	218, 235, 270, 332, 510
CseI	GACGC	1 cut(s)	551
Csp6I	GTAC	2 cut(s)	73, 189
CviAII	CATG	1 cut(s)	313
CviQI	GTAC	2 cut(s)	73, 189
DpnI	GATC	2 cut(s)	80, 553
DpnII	GATC	2 cut(s)	78, 551
DraI	TTTAAA	1 cut(s)	399
DrdI	GACNNNNNNGTC	1 cut(s)	299
DseDI	GACNNNNNNGTC	1 cut(s)	299
Eco24I	GRGCYC	1 cut(s)	179
Eco47I	GGWCC	2 cut(s)	218, 332
EcoRII	CCWGG	1 cut(s)	179
EcoT38I	GRGCYC	1 cut(s)	179
FaeI	CATG	1 cut(s)	316
FaiI	YATR	4 cut(s)	275, 314, 318, 539
FalI	AAGNNNNNCTT	2 cut(s)	248, 280
FaqI	GGGAC	1 cut(s)	204
FatI	CATG	1 cut(s)	312
FblI	GTMKAC	2 cut(s)	252, 317
Fnu4HI	GCNGC	6 cut(s)	18, 66, 112, 138, 141, 285
FokI	GGATG	1 cut(s)	31
FriOI	GRGCYC	1 cut(s)	179
Fsp4HI	GCNGC	6 cut(s)	18, 66, 112, 138, 141, 285
GlaI	GCGC	2 cut(s)	43, 64
GluI	GCNGC	6 cut(s)	18, 66, 112, 138, 141, 285
HaeIII	GGCC	4 cut(s)	237, 271, 284, 512
HapII	CCGG	1 cut(s)	288
HgaI	GACGC	1 cut(s)	551
HhaI	GCGC	2 cut(s)	44, 65
Hin1II	CATG	1 cut(s)	316
Hin6I	GCGC	2 cut(s)	42, 63
HinP1I	GCGC	2 cut(s)	42, 63
HincII	GTYRAC	1 cut(s)	253
HindII	GTYRAC	1 cut(s)	253
HindIII	AAGCTT	1 cut(s)	401
HpaII	CCGG	1 cut(s)	288
HphI	GGTGA	1 cut(s)	514
Hpy166II	GTNNAC	4 cut(s)	189, 253, 318, 332
Hpy188III	TCNNGA	3 cut(s)	362, 383, 469
Hpy8I	GTNNAC	4 cut(s)	189, 253, 318, 332
Hpy99I	CGWCG	3 cut(s)	41, 305, 524
HpyAV	CCTTC	4 cut(s)	274, 430, 472, 499
HpyCH4III	ACNGT	4 cut(s)	193, 214, 226, 301
HpyCH4V	TGCA	2 cut(s)	10, 339
HpyF10VI	GCNNNNNNNGC	2 cut(s)	39, 542
Hsp92II	CATG	1 cut(s)	316
HspAI	GCGC	2 cut(s)	42, 63
KroI	GCCGGC	1 cut(s)	287
KroNI	GCCGGC	1 cut(s)	289
Kzo9I	GATC	2 cut(s)	78, 551
LmnI	GCTCC	5 cut(s)	38, 92, 182, 355, 439
LpnPI	CCDG	5 cut(s)	73, 166, 193, 253, 301
Lsp1109I	GCAGC	2 cut(s)	98, 127
LweI	GCATC	1 cut(s)	53
MaeIII	GTNAC	1 cut(s)	241
MalI	GATC	2 cut(s)	80, 553
MboI	GATC	2 cut(s)	78, 551
MboII	GAAGA	2 cut(s)	142, 181
MhlI	GDGCHC	1 cut(s)	179
MluCI	AATT	4 cut(s)	150, 356, 365, 515
MmeI	TCCRAC	1 cut(s)	127
MnlI	CCTC	8 cut(s)	39, 100, 103, 120, 159, 241, 317, 493
MreI	CGCCGGCG	1 cut(s)	287
MroNI	GCCGGC	1 cut(s)	287
MseI	TTAA	1 cut(s)	398
MspI	CCGG	1 cut(s)	288
MspR9I	CCNGG	1 cut(s)	181
Mva1269I	GAATGC	1 cut(s)	139
MvaI	CCWGG	1 cut(s)	181
MwoI	GCNNNNNNNGC	2 cut(s)	39, 542
NaeI	GCCGGC	1 cut(s)	289
NdeII	GATC	2 cut(s)	78, 551
NgoMIV	GCCGGC	1 cut(s)	287
NlaIII	CATG	1 cut(s)	316
NlaIV	GGNNCC	4 cut(s)	178, 220, 459, 511
NmuCI	GTSAC	1 cut(s)	241
NspI	RCATGY	1 cut(s)	316
PciI	ACATGT	1 cut(s)	312
PcsI	WCGNNNNNNNCGW	1 cut(s)	197
PctI	GAATGC	1 cut(s)	139
PdiI	GCCGGC	1 cut(s)	289
PflFI	GACNNNGTC	1 cut(s)	212
PkrI	GCNGC	6 cut(s)	19, 67, 113, 139, 142, 286
PscI	ACATGT	1 cut(s)	312
PshAI	GACNNNNGTC	1 cut(s)	302
Psp6I	CCWGG	1 cut(s)	179
PspGI	CCWGG	1 cut(s)	179
PspN4I	GGNNCC	4 cut(s)	178, 220, 459, 511
PspPI	GGNCC	5 cut(s)	218, 235, 270, 332, 510
PstI	CTGCAG	1 cut(s)	12
PsyI	GACNNNGTC	1 cut(s)	212
RsaI	GTAC	2 cut(s)	74, 190
RsaNI	GTAC	2 cut(s)	73, 189
SalI	GTCGAC	1 cut(s)	251
SaqAI	TTAA	1 cut(s)	398
SatI	GCNGC	6 cut(s)	18, 66, 112, 138, 141, 285
Sau3AI	GATC	2 cut(s)	78, 551
Sau96I	GGNCC	5 cut(s)	218, 235, 270, 332, 510
ScrFI	CCNGG	1 cut(s)	181
SduI	GDGCHC	1 cut(s)	179
SfaNI	GCATC	1 cut(s)	53
SfcI	CTRYAG	2 cut(s)	8, 537
SgrAI	CRCCGGYG	1 cut(s)	287
SinI	GGWCC	2 cut(s)	218, 332
Sse9I	AATT	4 cut(s)	150, 356, 365, 515
SsiI	CCGC	5 cut(s)	18, 66, 137, 143, 285
StyD4I	CCNGG	1 cut(s)	179
TaaI	ACNGT	4 cut(s)	193, 214, 226, 301
TaqI	TCGA	7 cut(s)	101, 107, 200, 252, 309, 519, 554
TaqII	GACCGA	1 cut(s)	206
TasI	AATT	4 cut(s)	150, 356, 365, 515
TauI	GCSGC	4 cut(s)	20, 68, 140, 287
Tru1I	TTAA	1 cut(s)	398
Tru9I	TTAA	1 cut(s)	398
TscAI	CASTG	2 cut(s)	217, 229
TseFI	GTSAC	1 cut(s)	241
TseI	GCWGC	2 cut(s)	111, 140
Tsp45I	GTSAC	1 cut(s)	241
TspGWI	ACGGA	2 cut(s)	25, 260
TspRI	CASTG	2 cut(s)	217, 229
Tth111I	GACNNNGTC	1 cut(s)	212
VpaK11BI	GGWCC	2 cut(s)	218, 332
XapI	RAATTY	2 cut(s)	356, 515
XceI	RCATGY	1 cut(s)	316
XcmI	CCANNNNNNNNNTGG	1 cut(s)	229
XmiI	GTMKAC	2 cut(s)	252, 317

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.