MD05G1014300.v1.1

Belongs to the iron ascorbate-dependent oxidoreductase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0006926
Physical Location & Seq
Forward (+)
2675960 .. 2682144
6185 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1014300.v1.1.491

Sequence Viewer

Length: 534 bp
ATGGATATCAAACTTGTAGGGCGTGGAGAATACGACCGAGCCACAGAGATAAAAGCTTTCGATGATACAAAAGCCGGTGTGAAAGGCCTCGTTGATGCTGGCACAGTAAATGTACCCAAGATATTCATCCGTCCACCCAATGAGCAAACCCAGTTTGCCACGTGTCACCCACCCAACATGCAAATTCCTGTCATCGATCTCCACGGCATCGATGATAGTGATGACCTCCGCAAGCAGATTGTGGATGAAGTTCGGATCGCGTCCAAAACATGGGGATTCTTCCAAGTGGTTAATCATGGAGTTCCAGTGAGTGTGCTGGAAGATATGATCCATGGAGTAATCAAGTTTAATGAGCAGGATCTTGATGCTAAGAAAGTGTGGTATAGCCGTGATCGAGCAAAAACCGTCAGATTTAATAGCAACTATGATCTGTACCACGCACGAGCAGCTAATTGGAGAGACACTCTGTCTTGTACCAGGCCCGCAGTTGATGATCCCAAACAACTCCCTTCAGTTTGCAGGGATGATGGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
2OG-FeII_Oxy DIOX_N

Protein Analysis

178

Amino Acids

20.02

Weight (kDa)

5.94

Isoelectric Point (pI)

27.42

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DIOX_N PF14226 62 - 168 4.4e-18 non-haem dioxygenase in morphine synthesis N-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 270
AccII CGCG 1 cut(s) 260
AciI CCGC 2 cut(s) 229, 483
AclWI GGATC 4 cut(s) 263, 322, 366, 488
AcsI RAATTY 1 cut(s) 183
AcuI CTGAAG 1 cut(s) 495
AcvI CACGTG 1 cut(s) 162
AfaI GTAC 3 cut(s) 114, 434, 475
AfiI CCNNNNNNNGG 1 cut(s) 270
AflIII ACRYGT 1 cut(s) 161
AhdI GACNNNNNGTC 1 cut(s) 466
AjnI CCWGG 1 cut(s) 476
AluBI AGCT 2 cut(s) 56, 449
AluI AGCT 2 cut(s) 56, 449
Alw26I GTCTC 1 cut(s) 453
AlwI GGATC 4 cut(s) 263, 322, 366, 488
AoxI GGCC 2 cut(s) 85, 479
ApeKI GCWGC 1 cut(s) 446
ApoI RAATTY 1 cut(s) 183
AspS9I GGNCC 1 cut(s) 480
AsuHPI GGTGA 1 cut(s) 158
BauI CACGAG 1 cut(s) 441
BbrPI CACGTG 1 cut(s) 162
BbvI GCAGC 1 cut(s) 458
BccI CCATC 1 cut(s) 521
BceAI ACGGC 2 cut(s) 220, 372
BciT130I CCWGG 1 cut(s) 478
BcoDI GTCTC 1 cut(s) 453
BfaI CTAG 1 cut(s) 532
BisI GCNGC 1 cut(s) 447
BlsI GCNGC 1 cut(s) 448
Bme1390I CCNGG 1 cut(s) 478
BmeRI GACNNNNNGTC 1 cut(s) 466
BmgT120I GGNCC 1 cut(s) 480
BmrFI CCNGG 1 cut(s) 478
BmrI ACTGGG 1 cut(s) 145
BmsI GCATC 3 cut(s) 85, 216, 355
BmuI ACTGGG 1 cut(s) 145
BplI GAGNNNNNCTC 2 cut(s) 448, 480
Bsa29I ATCGAT 2 cut(s) 195, 210
BsaAI YACGTR 1 cut(s) 162
BsaBI GATNNNNATC 1 cut(s) 125
BsaJI CCNNGG 2 cut(s) 202, 331
BsaXI ACNNNNNCTCC 2 cut(s) 291, 321
Bsc4I CCNNNNNNNGG 1 cut(s) 270
Bse118I RCCGGY 1 cut(s) 74
Bse1I ACTGG 2 cut(s) 151, 305
Bse8I GATNNNNATC 1 cut(s) 125
BseBI CCWGG 1 cut(s) 478
BseCI ATCGAT 2 cut(s) 195, 210
BseDI CCNNGG 2 cut(s) 202, 331
BseGI GGATG 3 cut(s) 126, 250, 529
BseJI GATNNNNATC 1 cut(s) 125
BseLI CCNNNNNNNGG 1 cut(s) 270
BseNI ACTGG 2 cut(s) 151, 305
BseXI GCAGC 1 cut(s) 458
Bsh1236I CGCG 1 cut(s) 260
Bsh1285I CGRYCG 1 cut(s) 37
BshFI GGCC 2 cut(s) 87, 481
BshVI ATCGAT 2 cut(s) 195, 210
BsiEI CGRYCG 1 cut(s) 37
BsiSI CCGG 1 cut(s) 75
BslI CCNNNNNNNGG 1 cut(s) 270
BsmAI GTCTC 1 cut(s) 453
BsnI GGCC 2 cut(s) 87, 481
Bsp143I GATC 7 cut(s) 196, 255, 327, 358, 391, 427, 493
Bsp19I CCATGG 1 cut(s) 331
BspACI CCGC 2 cut(s) 229, 483
BspANI GGCC 2 cut(s) 87, 481
BspDI ATCGAT 2 cut(s) 195, 210
BspFNI CGCG 1 cut(s) 260
BspPI GGATC 4 cut(s) 263, 322, 366, 488
BsrFI RCCGGY 1 cut(s) 74
BsrI ACTGG 2 cut(s) 151, 305
BssAI RCCGGY 1 cut(s) 74
BssECI CCNNGG 2 cut(s) 202, 331
BssMI GATC 7 cut(s) 196, 255, 327, 358, 391, 427, 493
BssSI CACGAG 1 cut(s) 441
BssT1I CCWWGG 1 cut(s) 331
Bst2BI CACGAG 1 cut(s) 441
Bst2UI CCWGG 1 cut(s) 478
Bst4CI ACNGT 2 cut(s) 106, 406
BstBAI YACGTR 1 cut(s) 162
BstC8I GCNNGC 3 cut(s) 100, 233, 483
BstDEI CTNAG 1 cut(s) 369
BstDSI CCRYGG 2 cut(s) 202, 331
BstF5I GGATG 3 cut(s) 126, 250, 529
BstFNI CGCG 1 cut(s) 260
BstKTI GATC 7 cut(s) 199, 258, 330, 361, 394, 430, 496
BstMAI GTCTC 1 cut(s) 453
BstMBI GATC 7 cut(s) 196, 255, 327, 358, 391, 427, 493
BstMCI CGRYCG 1 cut(s) 37
BstMWI GCNNNNNNNGC 1 cut(s) 446
BstNI CCWGG 1 cut(s) 478
BstNSI RCATGY 1 cut(s) 181
BstSCI CCNGG 1 cut(s) 476
BstUI CGCG 1 cut(s) 260
BstV1I GCAGC 1 cut(s) 458
BstX2I RGATCY 1 cut(s) 358
BstYI RGATCY 1 cut(s) 358
Bsu15I ATCGAT 2 cut(s) 195, 210
BsuRI GGCC 2 cut(s) 87, 481
BsuTUI ATCGAT 2 cut(s) 195, 210
BtgI CCRYGG 2 cut(s) 202, 331
BtsCI GGATG 3 cut(s) 126, 250, 529
BtsIMutI CAGTG 1 cut(s) 312
Cac8I GCNNGC 3 cut(s) 100, 233, 483
Cfr10I RCCGGY 1 cut(s) 74
Cfr13I GGNCC 1 cut(s) 480
ClaI ATCGAT 2 cut(s) 195, 210
CseI GACGC 1 cut(s) 249
Csp6I GTAC 3 cut(s) 113, 433, 474
CviAII CATG 4 cut(s) 178, 270, 296, 332
CviJI RGCY 8 cut(s) 41, 56, 74, 87, 387, 449, 481, 531
CviKI_1 RGCY 8 cut(s) 41, 56, 74, 87, 387, 449, 481, 531
CviQI GTAC 3 cut(s) 113, 433, 474
DdeI CTNAG 1 cut(s) 369
DpnI GATC 7 cut(s) 198, 257, 329, 360, 393, 429, 495
DpnII GATC 7 cut(s) 196, 255, 327, 358, 391, 427, 493
DriI GACNNNNNGTC 1 cut(s) 466
Eam1105I GACNNNNNGTC 1 cut(s) 466
Eco130I CCWWGG 1 cut(s) 331
Eco147I AGGCCT 1 cut(s) 87
Eco32I GATATC 1 cut(s) 7
Eco57I CTGAAG 1 cut(s) 495
Eco72I CACGTG 1 cut(s) 162
EcoRII CCWGG 1 cut(s) 476
EcoRV GATATC 1 cut(s) 7
EcoT14I CCWWGG 1 cut(s) 331
ErhI CCWWGG 1 cut(s) 331
FaeI CATG 4 cut(s) 181, 273, 299, 335
FaiI YATR 7 cut(s) 179, 271, 297, 326, 333, 384, 426
FatI CATG 4 cut(s) 177, 269, 295, 331
FauI CCCGC 1 cut(s) 490
Fnu4HI GCNGC 1 cut(s) 447
FokI GGATG 2 cut(s) 113, 257
Fsp4HI GCNGC 1 cut(s) 447
FspBI CTAG 1 cut(s) 532
GluI GCNGC 1 cut(s) 447
HaeIII GGCC 2 cut(s) 87, 481
HapII CCGG 1 cut(s) 75
HgaI GACGC 1 cut(s) 249
Hin1II CATG 4 cut(s) 181, 273, 299, 335
HindIII AAGCTT 1 cut(s) 54
HinfI GANTC 1 cut(s) 276
HpaII CCGG 1 cut(s) 75
HphI GGTGA 1 cut(s) 158
Hpy166II GTNNAC 1 cut(s) 134
Hpy188I TCNGA 2 cut(s) 255, 410
Hpy188III TCNNGA 1 cut(s) 362
Hpy8I GTNNAC 1 cut(s) 134
HpyAV CCTTC 1 cut(s) 519
HpyCH4III ACNGT 2 cut(s) 106, 406
HpyCH4IV ACGT 1 cut(s) 161
HpyCH4V TGCA 2 cut(s) 181, 519
HpyF10VI GCNNNNNNNGC 1 cut(s) 446
HpyF3I CTNAG 1 cut(s) 369
HpySE526I ACGT 1 cut(s) 161
Hsp92II CATG 4 cut(s) 181, 273, 299, 335
Kzo9I GATC 7 cut(s) 196, 255, 327, 358, 391, 427, 493
Lsp1109I GCAGC 1 cut(s) 458
LweI GCATC 3 cut(s) 85, 216, 355
MaeI CTAG 1 cut(s) 532
MaeII ACGT 1 cut(s) 161
MaeIII GTNAC 1 cut(s) 164
MalI GATC 7 cut(s) 198, 257, 329, 360, 393, 429, 495
MboI GATC 7 cut(s) 196, 255, 327, 358, 391, 427, 493
MboII GAAGA 2 cut(s) 271, 332
MflI RGATCY 1 cut(s) 358
MluCI AATT 2 cut(s) 183, 451
MnlI CCTC 2 cut(s) 98, 236
MseI TTAA 3 cut(s) 291, 348, 414
MspI CCGG 1 cut(s) 75
MspR9I CCNGG 1 cut(s) 478
MvaI CCWGG 1 cut(s) 478
MvnI CGCG 1 cut(s) 260
MwoI GCNNNNNNNGC 1 cut(s) 446
NcoI CCATGG 1 cut(s) 331
NdeII GATC 7 cut(s) 196, 255, 327, 358, 391, 427, 493
NlaIII CATG 4 cut(s) 181, 273, 299, 335
NmuCI GTSAC 1 cut(s) 164
NspI RCATGY 1 cut(s) 181
PceI AGGCCT 1 cut(s) 87
PfeI GAWTC 1 cut(s) 276
PflMI CCANNNNNTGG 1 cut(s) 270
PkrI GCNGC 1 cut(s) 448
PmaCI CACGTG 1 cut(s) 162
PmlI CACGTG 1 cut(s) 162
Ppu21I YACGTR 1 cut(s) 162
Psp6I CCWGG 1 cut(s) 476
PspCI CACGTG 1 cut(s) 162
PspGI CCWGG 1 cut(s) 476
PspPI GGNCC 1 cut(s) 480
PsuI RGATCY 1 cut(s) 358
RsaI GTAC 3 cut(s) 114, 434, 475
RsaNI GTAC 3 cut(s) 113, 433, 474
SaqAI TTAA 3 cut(s) 291, 348, 414
SatI GCNGC 1 cut(s) 447
Sau3AI GATC 7 cut(s) 196, 255, 327, 358, 391, 427, 493
Sau96I GGNCC 1 cut(s) 480
ScrFI CCNGG 1 cut(s) 478
SetI ASST 4 cut(s) 58, 164, 228, 451
SfaNI GCATC 3 cut(s) 85, 216, 355
Sse9I AATT 2 cut(s) 183, 451
SseBI AGGCCT 1 cut(s) 87
SsiI CCGC 2 cut(s) 229, 483
SspMI CTAG 1 cut(s) 532
StuI AGGCCT 1 cut(s) 87
StyD4I CCNGG 1 cut(s) 476
StyI CCWWGG 1 cut(s) 331
TaaI ACNGT 2 cut(s) 106, 406
TaiI ACGT 1 cut(s) 164
TaqI TCGA 4 cut(s) 60, 195, 210, 394
TaqII GACCGA 1 cut(s) 51
TasI AATT 2 cut(s) 183, 451
TfiI GAWTC 1 cut(s) 276
Tru1I TTAA 3 cut(s) 291, 348, 414
Tru9I TTAA 3 cut(s) 291, 348, 414
TscAI CASTG 1 cut(s) 312
TseFI GTSAC 1 cut(s) 164
TseI GCWGC 1 cut(s) 446
Tsp45I GTSAC 1 cut(s) 164
TspDTI ATGAA 2 cut(s) 115, 261
TspGWI ACGGA 1 cut(s) 119
TspRI CASTG 1 cut(s) 312
Van91I CCANNNNNTGG 1 cut(s) 270
XapI RAATTY 1 cut(s) 183
XceI RCATGY 1 cut(s) 181
XspI CTAG 1 cut(s) 532
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.