Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr05

Orthogroup ID

OG0003869

Physical Location & Seq

Genomic Coordinates Forward (+)

3829378 .. 3830009

Gene Structure 632 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD05G1022700.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 519 bp

ATGACAACTTCGCCGACTGCGATAAAGCTCGCCAAAGCTTTCCTGCAGGAGCTCAACACGTTCGAGATTCCGCTCCTCAAGAGCAAGGCCGTCGTGCATGCGAATCTCCGGGAGAAGCACAACTTCGACGAGCTGAGGGAGGAGATTAACCGGCAGATTGTGCAGGCGAAGACCGACATTGAGACGCTCAAGGAGCAGCTCGAGGAGAGTAAGATCGAAAGAATGCACAAGGAGGAATGTGAGTCGATCAGGAAATTGATTGCTACGCAGCCGCCGCGGTCGGAGACGCTCAAGATCATTTCAGATTCGGAGAAGGAGATTATGGCATTGGATGCGGAGAACACCCCAAGTTGGCGGACGCTGGATCTGAGAAAGAAGCAGTTTGCTCTGCTCCTGCATGTGGTGGATGAGTTGCAGAATACCGTAGAGGAAGAACAGAGGAGTTTAATTGAGGAGAAAGAGCAGAAAAATGCAATGGAAGGTGCAGCTGGGGGCTCGGAACCCATGCATGTTGATTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000346 GO:0000347 GO:0000445 GO:0000781 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005694 GO:0006403 GO:0006405 GO:0006406 GO:0006611 GO:0006810 GO:0006886 GO:0006913 GO:0008104 GO:0008150 GO:0008152 GO:0010467 GO:0015031 GO:0015833 GO:0015931 GO:0031503 GO:0032991 GO:0033036 GO:0034613 GO:0042886 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044446 GO:0044464 GO:0045184 GO:0046907 GO:0050657 GO:0050658 GO:0051028 GO:0051168 GO:0051169 GO:0051179 GO:0051234 GO:0051236 GO:0051641 GO:0051649 GO:0070727 GO:0071166 GO:0071426 GO:0071427 GO:0071702 GO:0071704 GO:0071705 GO:0098687

KEGG Pathways

Metabolic & Signaling

ko03013 map03013

Pfam Domains

Protein Families

THOC7

Protein Analysis

173

Amino Acids

19.8

Weight (kDa)

5.2

Isoelectric Point (pI)

69.03

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
THOC7	PF05615	8 - 98	4e-22	Tho complex subunit 7

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0003869)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G02950 AT3G02950 AT5G16790 AT5G16790
fragaria_vesca	FvH4_2g13990 FvH4_4g00100
malus_domestica	MD00G1125800.v1.1 MD05G1022700.v1.1 MD10G1023900.v1.1
prunus_persica	Prupe.8G026900_v2.0.a1 Prupe.8G026900_v2.0.a1 Prupe.8G027200_v2.0.a1 Prupe.8G027200_v2.0.a1 Prupe.8G027200_v2.0.a1 Prupe.8G027400_v2.0.a1
pyrus_communis	pycom10g01580 pycom11g17910
rosa_chinensis	RchiOBHm_Chr6g0275831
rosa_laevigata	RLG00000013429
rosa_multiflora	Rmu_sc0002030.1_g000025 Rmu_sc0006781.1_g000001 Rmu_sc0006781.1_g000002
rosa_roxburghii	Rroxscaffold_7G00192820
rosa_rugosa	Rorug06G0096300
rosa_samantha	Rh6AG208500 Rh6AG210200 Rh6BG211100 Rh6BG212500 Rh6CG214600 Rh6DG204500
rosa_wichuraiana	Rw0G021610 Rw0G021620 Rw6G018170 Rw6G018300

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccBSI	CCGCTC	1 cut(s)	73
AccII	CGCG	1 cut(s)	277
AciI	CCGC	6 cut(s)	71, 272, 275, 277, 335, 355
AclWI	GGATC	1 cut(s)	372
AfiI	CCNNNNNNNGG	2 cut(s)	351, 400
AflIII	ACRYGT	1 cut(s)	57
AluBI	AGCT	6 cut(s)	28, 38, 52, 133, 199, 488
AluI	AGCT	6 cut(s)	28, 38, 52, 133, 199, 488
Alw21I	GWGCWC	1 cut(s)	54
Alw26I	GTCTC	2 cut(s)	176, 278
AlwI	GGATC	1 cut(s)	372
Ama87I	CYCGRG	1 cut(s)	200
AoxI	GGCC	1 cut(s)	87
ApeKI	GCWGC	3 cut(s)	196, 268, 485
AsuC2I	CCSGG	1 cut(s)	110
AvaI	CYCGRG	1 cut(s)	200
BanII	GRGCYC	2 cut(s)	54, 497
BbsI	GAAGAC	1 cut(s)	176
Bbv12I	GWGCWC	1 cut(s)	54
BbvCI	CCTCAGC	1 cut(s)	134
BbvI	GCAGC	3 cut(s)	208, 280, 497
BceAI	ACGGC	1 cut(s)	74
BcgI	CGANNNNNNTGC	2 cut(s)	73, 107
BcnI	CCSGG	1 cut(s)	110
BcoDI	GTCTC	2 cut(s)	176, 278
BfmI	CTRYAG	1 cut(s)	44
BisI	GCNGC	5 cut(s)	197, 269, 272, 275, 486
BlsI	GCNGC	5 cut(s)	198, 270, 273, 276, 487
Bme1390I	CCNGG	1 cut(s)	110
BmeT110I	CYCGRG	1 cut(s)	200
BmiI	GGNNCC	1 cut(s)	501
BmrFI	CCNGG	1 cut(s)	110
BmsI	GCATC	1 cut(s)	322
BpiI	GAAGAC	1 cut(s)	176
Bpu10I	CCTNAGC	1 cut(s)	134
BpuEI	CTTGAG	3 cut(s)	62, 173, 275
BpuMI	CCSGG	1 cut(s)	110
BsaJI	CCNNGG	1 cut(s)	275
Bsc4I	CCNNNNNNNGG	2 cut(s)	351, 400
Bse118I	RCCGGY	1 cut(s)	150
Bse3DI	GCAATG	1 cut(s)	480
BseDI	CCNNGG	1 cut(s)	275
BseGI	GGATG	2 cut(s)	337, 412
BseLI	CCNNNNNNNGG	2 cut(s)	351, 400
BseMI	GCAATG	1 cut(s)	480
BseMII	CTCAG	2 cut(s)	125, 359
BseRI	GAGGAG	5 cut(s)	65, 155, 218, 454, 467
BseXI	GCAGC	3 cut(s)	208, 280, 497
BseYI	CCCAGC	1 cut(s)	488
BsgI	GTGCAG	2 cut(s)	182, 504
Bsh1236I	CGCG	1 cut(s)	277
Bsh1285I	CGRYCG	1 cut(s)	281
BshFI	GGCC	1 cut(s)	89
BsiEI	CGRYCG	1 cut(s)	281
BsiHKAI	GWGCWC	1 cut(s)	54
BsiHKCI	CYCGRG	1 cut(s)	200
BsiSI	CCGG	2 cut(s)	109, 151
BslI	CCNNNNNNNGG	2 cut(s)	351, 400
BsmAI	GTCTC	2 cut(s)	176, 278
BsmBI	CGTCTC	2 cut(s)	176, 278
BsmI	GAATGC	1 cut(s)	228
BsnI	GGCC	1 cut(s)	89
BsoBI	CYCGRG	1 cut(s)	200
Bsp1286I	GDGCHC	2 cut(s)	54, 497
Bsp143I	GATC	4 cut(s)	213, 246, 294, 364
BspACI	CCGC	6 cut(s)	71, 272, 275, 277, 335, 355
BspANI	GGCC	1 cut(s)	89
BspCNI	CTCAG	2 cut(s)	126, 360
BspFNI	CGCG	1 cut(s)	277
BspLI	GGNNCC	1 cut(s)	501
BspMAI	CTGCAG	1 cut(s)	48
BspPI	GGATC	1 cut(s)	372
BsrBI	CCGCTC	1 cut(s)	73
BsrDI	GCAATG	1 cut(s)	480
BsrFI	RCCGGY	1 cut(s)	150
BssAI	RCCGGY	1 cut(s)	150
BssECI	CCNNGG	1 cut(s)	275
BssMI	GATC	4 cut(s)	213, 246, 294, 364
Bst4CI	ACNGT	1 cut(s)	424
BstAPI	GCANNNNNTGC	2 cut(s)	160, 332
BstC8I	GCNNGC	3 cut(s)	30, 99, 165
BstDEI	CTNAG	2 cut(s)	134, 368
BstDSI	CCRYGG	1 cut(s)	275
BstF5I	GGATG	2 cut(s)	337, 412
BstFNI	CGCG	1 cut(s)	277
BstKTI	GATC	4 cut(s)	216, 249, 297, 367
BstMAI	GTCTC	2 cut(s)	176, 278
BstMBI	GATC	4 cut(s)	213, 246, 294, 364
BstMCI	CGRYCG	1 cut(s)	281
BstMWI	GCNNNNNNNGC	4 cut(s)	160, 193, 274, 332
BstNSI	RCATGY	3 cut(s)	101, 401, 512
BstSCI	CCNGG	1 cut(s)	108
BstSFI	CTRYAG	1 cut(s)	44
BstUI	CGCG	1 cut(s)	277
BstV1I	GCAGC	3 cut(s)	208, 280, 497
BstV2I	GAAGAC	1 cut(s)	176
BstX2I	RGATCY	1 cut(s)	364
BstYI	RGATCY	1 cut(s)	364
BsuRI	GGCC	1 cut(s)	89
BtgI	CCRYGG	1 cut(s)	275
BtsCI	GGATG	2 cut(s)	337, 412
Cac8I	GCNNGC	3 cut(s)	30, 99, 165
Cfr10I	RCCGGY	1 cut(s)	150
Cfr42I	CCGCGG	1 cut(s)	278
CseI	GACGC	3 cut(s)	193, 295, 367
CviAII	CATG	4 cut(s)	98, 398, 505, 509
CviJI	RGCY	9 cut(s)	28, 38, 52, 89, 133, 199, 271, 488, 495
CviKI_1	RGCY	9 cut(s)	28, 38, 52, 89, 133, 199, 271, 488, 495
DdeI	CTNAG	2 cut(s)	134, 368
DpnI	GATC	4 cut(s)	215, 248, 296, 366
DpnII	GATC	4 cut(s)	213, 246, 294, 364
EciI	GGCGGA	1 cut(s)	370
Ecl136II	GAGCTC	1 cut(s)	52
Eco24I	GRGCYC	2 cut(s)	54, 497
Eco53kI	GAGCTC	1 cut(s)	52
Eco88I	CYCGRG	1 cut(s)	200
EcoICRI	GAGCTC	1 cut(s)	52
EcoT22I	ATGCAT	1 cut(s)	510
EcoT38I	GRGCYC	2 cut(s)	54, 497
Esp3I	CGTCTC	2 cut(s)	176, 278
FaeI	CATG	4 cut(s)	101, 401, 508, 512
FaiI	YATR	5 cut(s)	99, 323, 399, 506, 510
FalI	AAGNNNNNCTT	2 cut(s)	107, 139
FatI	CATG	4 cut(s)	97, 397, 504, 508
Fnu4HI	GCNGC	5 cut(s)	197, 269, 272, 275, 486
FokI	GGATG	2 cut(s)	344, 419
FriOI	GRGCYC	2 cut(s)	54, 497
Fsp4HI	GCNGC	5 cut(s)	197, 269, 272, 275, 486
GluI	GCNGC	5 cut(s)	197, 269, 272, 275, 486
GsaI	CCCAGC	1 cut(s)	492
HaeIII	GGCC	1 cut(s)	89
HapII	CCGG	2 cut(s)	109, 151
HgaI	GACGC	3 cut(s)	193, 295, 367
Hin1II	CATG	4 cut(s)	101, 401, 508, 512
HindIII	AAGCTT	1 cut(s)	36
HinfI	GANTC	4 cut(s)	67, 103, 242, 305
HpaII	CCGG	2 cut(s)	109, 151
Hpy188I	TCNGA	5 cut(s)	283, 304, 310, 369, 499
Hpy188III	TCNNGA	4 cut(s)	64, 79, 250, 292
Hpy99I	CGWCG	2 cut(s)	95, 131
HpyAV	CCTTC	2 cut(s)	307, 473
HpyCH4III	ACNGT	1 cut(s)	424
HpyCH4IV	ACGT	1 cut(s)	59
HpyCH4V	TGCA	9 cut(s)	46, 97, 163, 226, 397, 415, 473, 485, 508
HpyF10VI	GCNNNNNNNGC	4 cut(s)	160, 193, 274, 332
HpyF3I	CTNAG	2 cut(s)	134, 368
HpySE526I	ACGT	1 cut(s)	59
Hsp92II	CATG	4 cut(s)	101, 401, 508, 512
KspI	CCGCGG	1 cut(s)	278
Kzo9I	GATC	4 cut(s)	213, 246, 294, 364
LmnI	GCTCC	4 cut(s)	49, 78, 193, 396
LpnPI	CCDG	9 cut(s)	32, 56, 122, 149, 164, 235, 347, 407, 474
Lsp1109I	GCAGC	3 cut(s)	208, 280, 497
LweI	GCATC	1 cut(s)	322
MaeII	ACGT	1 cut(s)	59
MalI	GATC	4 cut(s)	215, 248, 296, 366
MbiI	CCGCTC	1 cut(s)	73
MboI	GATC	4 cut(s)	213, 246, 294, 364
MboII	GAAGA	2 cut(s)	181, 443
MflI	RGATCY	1 cut(s)	364
MhlI	GDGCHC	2 cut(s)	54, 497
MluCI	AATT	2 cut(s)	254, 447
MlyI	GAGTC	1 cut(s)	251
MmeI	TCCRAC	1 cut(s)	261
MnlI	CCTC	8 cut(s)	86, 129, 133, 196, 226, 421, 432, 445
Mph1103I	ATGCAT	1 cut(s)	510
MseI	TTAA	2 cut(s)	147, 446
MspA1I	CMGCKG	2 cut(s)	277, 488
MspI	CCGG	2 cut(s)	109, 151
MspR9I	CCNGG	1 cut(s)	110
Mva1269I	GAATGC	1 cut(s)	228
MvnI	CGCG	1 cut(s)	277
MwoI	GCNNNNNNNGC	4 cut(s)	160, 193, 274, 332
NciI	CCSGG	1 cut(s)	110
NdeII	GATC	4 cut(s)	213, 246, 294, 364
NlaIII	CATG	4 cut(s)	101, 401, 508, 512
NlaIV	GGNNCC	1 cut(s)	501
NsiI	ATGCAT	1 cut(s)	510
NspI	RCATGY	3 cut(s)	101, 401, 512
PaeI	GCATGC	1 cut(s)	101
PaeR7I	CTCGAG	1 cut(s)	200
PcsI	WCGNNNNNNNCGW	1 cut(s)	17
PctI	GAATGC	1 cut(s)	228
PfeI	GAWTC	3 cut(s)	67, 103, 305
PfoI	TCCNGGA	1 cut(s)	108
PkrI	GCNGC	5 cut(s)	198, 270, 273, 276, 487
PleI	GAGTC	1 cut(s)	250
PpsI	GAGTC	1 cut(s)	250
Psp124BI	GAGCTC	1 cut(s)	54
PspFI	CCCAGC	1 cut(s)	488
PspN4I	GGNNCC	1 cut(s)	501
PspXI	VCTCGAGB	1 cut(s)	200
PstI	CTGCAG	1 cut(s)	48
PsuI	RGATCY	1 cut(s)	364
PvuII	CAGCTG	1 cut(s)	488
SacI	GAGCTC	1 cut(s)	54
SacII	CCGCGG	1 cut(s)	278
SaqAI	TTAA	2 cut(s)	147, 446
SatI	GCNGC	5 cut(s)	197, 269, 272, 275, 486
Sau3AI	GATC	4 cut(s)	213, 246, 294, 364
SbfI	CCTGCAGG	1 cut(s)	48
SchI	GAGTC	1 cut(s)	251
ScrFI	CCNGG	1 cut(s)	110
SdaI	CCTGCAGG	1 cut(s)	48
SduI	GDGCHC	2 cut(s)	54, 497
SetI	ASST	8 cut(s)	30, 40, 54, 62, 135, 201, 484, 490
SfaNI	GCATC	1 cut(s)	322
SfcI	CTRYAG	1 cut(s)	44
Sfr274I	CTCGAG	1 cut(s)	200
Sfr303I	CCGCGG	1 cut(s)	278
SgrBI	CCGCGG	1 cut(s)	278
SlaI	CTCGAG	1 cut(s)	200
SmlI	CTYRAG	4 cut(s)	77, 188, 200, 290
SmoI	CTYRAG	4 cut(s)	77, 188, 200, 290
SphI	GCATGC	1 cut(s)	101
Sse8387I	CCTGCAGG	1 cut(s)	48
Sse9I	AATT	2 cut(s)	254, 447
SsiI	CCGC	6 cut(s)	71, 272, 275, 277, 335, 355
SstI	GAGCTC	1 cut(s)	54
StyD4I	CCNGG	1 cut(s)	108
TaaI	ACNGT	1 cut(s)	424
TaiI	ACGT	1 cut(s)	62
TaqI	TCGA	5 cut(s)	63, 126, 201, 216, 245
TaqII	GACCGA	1 cut(s)	188
TasI	AATT	2 cut(s)	254, 447
TauI	GCSGC	2 cut(s)	274, 277
TfiI	GAWTC	3 cut(s)	67, 103, 305
Tru1I	TTAA	2 cut(s)	147, 446
Tru9I	TTAA	2 cut(s)	147, 446
TseI	GCWGC	3 cut(s)	196, 268, 485
XceI	RCATGY	3 cut(s)	101, 401, 512
XhoI	CTCGAG	1 cut(s)	200
Zsp2I	ATGCAT	1 cut(s)	510

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.