Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr10

Orthogroup ID

OG0003869

Physical Location & Seq

Genomic Coordinates Forward (+)

2986408 .. 2987809

Gene Structure 1402 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD10G1023900.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 711 bp

ATGCTGCCGAAAGGAAGAAAAGTTTCAGGGCGAGGAGAGGCCGTGGCGGCGAACTACGCGTTCGGGCCACTCGAAGATGACGTCATCATCAAACACAGACTTCTTACTCGGACCACCACCACGAGAGGCGAACCACCATTGAAGAAGCTCCAGAAGAAGTTCACGTCCCTGTTCGTCGAGCTCGACAAGAACGATGACAACTTCGCCGACTGCGACAAGCTCGCCAAGGCTTTCCTCCAGGAGCTCAACACGTTCGAGATTCCGCTCCTCAAGAGCAAAGCCGTCGTGGATGCGAATCTCCGGGAGAAGCACAATTTTGACGAGCTGAGGGAGGAGATTAACCGGCAGATTGTGCAGGCGAAGACCGACATTGAGATGCTCAAGAAGCAGCTAGAGGAGAGTAAGATCGAAAGAAGGCACAAGGAGGAGTGTGAGTCGATCAGAAAATTGATTTCTACGCAGCCGCCGCGGTCGGAGACGCTCAAGATCATATCAGATTTGGAGAAGGAGATTGCGGCATTGGATGCGGAGAACACCGCGAGTTCACGGACGCTGGAGCTGAGGAAGAAGCAGTTTGCTCTGCTCCTGCATGTGGTGGATGAGTTGCAGAATACCATAGAGGAAGAGCAGAGGAGTTTAATTGAGGAGAAAGAGCAGAAAAATGGAATGGAGGATGCAACTGGGGGCTCAGAACCAATGCATGTTGACTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000346 GO:0000347 GO:0000445 GO:0000781 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005694 GO:0006403 GO:0006405 GO:0006406 GO:0006611 GO:0006810 GO:0006886 GO:0006913 GO:0008104 GO:0008150 GO:0008152 GO:0010467 GO:0015031 GO:0015833 GO:0015931 GO:0031503 GO:0032991 GO:0033036 GO:0034613 GO:0042886 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044446 GO:0044464 GO:0045184 GO:0046907 GO:0050657 GO:0050658 GO:0051028 GO:0051168 GO:0051169 GO:0051179 GO:0051234 GO:0051236 GO:0051641 GO:0051649 GO:0070727 GO:0071166 GO:0071426 GO:0071427 GO:0071702 GO:0071704 GO:0071705 GO:0098687

KEGG Pathways

Metabolic & Signaling

ko03013 map03013

Pfam Domains

Protein Families

THOC7

Protein Analysis

237

Amino Acids

27.05

Weight (kDa)

5.6

Isoelectric Point (pI)

54.3

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
THOC7	PF05615	27 - 162	3.2e-33	Tho complex subunit 7

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0003869)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G02950 AT3G02950 AT5G16790 AT5G16790
fragaria_vesca	FvH4_2g13990 FvH4_4g00100
malus_domestica	MD00G1125800.v1.1 MD05G1022700.v1.1 MD10G1023900.v1.1
prunus_persica	Prupe.8G026900_v2.0.a1 Prupe.8G026900_v2.0.a1 Prupe.8G027200_v2.0.a1 Prupe.8G027200_v2.0.a1 Prupe.8G027200_v2.0.a1 Prupe.8G027400_v2.0.a1
pyrus_communis	pycom10g01580 pycom11g17910
rosa_chinensis	RchiOBHm_Chr6g0275831
rosa_laevigata	RLG00000013429
rosa_multiflora	Rmu_sc0002030.1_g000025 Rmu_sc0006781.1_g000001 Rmu_sc0006781.1_g000002
rosa_roxburghii	Rroxscaffold_7G00192820
rosa_rugosa	Rorug06G0096300
rosa_samantha	Rh6AG208500 Rh6AG210200 Rh6BG211100 Rh6BG212500 Rh6CG214600 Rh6DG204500
rosa_wichuraiana	Rw0G021610 Rw0G021620 Rw6G018170 Rw6G018300

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	1 cut(s)	84
AccBSI	CCGCTC	1 cut(s)	265
AccII	CGCG	3 cut(s)	59, 469, 539
AciI	CCGC	8 cut(s)	47, 263, 464, 467, 469, 515, 527, 537
AcyI	GRCGYC	1 cut(s)	81
AfiI	CCNNNNNNNGG	1 cut(s)	592
AflIII	ACRYGT	2 cut(s)	57, 249
AgsI	TTSAA	1 cut(s)	142
AjiI	CACGTC	1 cut(s)	165
AjnI	CCWGG	1 cut(s)	237
AluBI	AGCT	7 cut(s)	148, 181, 220, 244, 325, 391, 559
AluI	AGCT	7 cut(s)	148, 181, 220, 244, 325, 391, 559
Alw21I	GWGCWC	2 cut(s)	183, 246
Alw26I	GTCTC	1 cut(s)	470
AoxI	GGCC	2 cut(s)	39, 65
ApeKI	GCWGC	3 cut(s)	4, 388, 460
ArsI	GACNNNNNNTTYG	2 cut(s)	66, 98
Asp700I	GAANNNNTTC	2 cut(s)	22, 158
AspS9I	GGNCC	2 cut(s)	65, 111
AsuC2I	CCSGG	1 cut(s)	302
AvaII	GGWCC	1 cut(s)	111
BanII	GRGCYC	3 cut(s)	183, 246, 689
BauI	CACGAG	1 cut(s)	121
BbsI	GAAGAC	1 cut(s)	368
Bbv12I	GWGCWC	2 cut(s)	183, 246
BbvCI	CCTCAGC	2 cut(s)	326, 560
BbvI	GCAGC	2 cut(s)	400, 472
BceAI	ACGGC	2 cut(s)	26, 266
BcgI	CGANNNNNNTGC	2 cut(s)	265, 299
BciT130I	CCWGG	1 cut(s)	239
BcnI	CCSGG	1 cut(s)	302
BcoDI	GTCTC	1 cut(s)	470
BfaI	CTAG	2 cut(s)	392, 709
BglI	GCCNNNNNGGC	1 cut(s)	47
BisI	GCNGC	7 cut(s)	5, 48, 389, 461, 464, 467, 516
BlsI	GCNGC	7 cut(s)	6, 49, 390, 462, 465, 468, 517
Bme1390I	CCNGG	2 cut(s)	239, 302
Bme18I	GGWCC	1 cut(s)	111
BmgBI	CACGTC	1 cut(s)	165
BmgT120I	GGNCC	2 cut(s)	65, 111
BmrFI	CCNGG	2 cut(s)	239, 302
BmrI	ACTGGG	1 cut(s)	690
BmsI	GCATC	4 cut(s)	280, 366, 514, 664
BmuI	ACTGGG	1 cut(s)	690
BpiI	GAAGAC	1 cut(s)	368
BpmI	CTGGAG	3 cut(s)	134, 221, 575
Bpu10I	CCTNAGC	2 cut(s)	326, 560
BpuEI	CTTGAG	3 cut(s)	254, 365, 467
BpuMI	CCSGG	1 cut(s)	302
BsaBI	GATNNNNATC	1 cut(s)	294
BsaHI	GRCGYC	1 cut(s)	81
BsaJI	CCNNGG	3 cut(s)	42, 225, 467
BsaXI	ACNNNNNCTCC	4 cut(s)	27, 57, 419, 449
Bsc4I	CCNNNNNNNGG	1 cut(s)	592
Bse118I	RCCGGY	1 cut(s)	342
Bse1I	ACTGG	1 cut(s)	685
Bse8I	GATNNNNATC	1 cut(s)	294
BseBI	CCWGG	1 cut(s)	239
BseDI	CCNNGG	3 cut(s)	42, 225, 467
BseGI	GGATG	4 cut(s)	295, 529, 604, 679
BseJI	GATNNNNATC	1 cut(s)	294
BseLI	CCNNNNNNNGG	1 cut(s)	592
BseMII	CTCAG	3 cut(s)	317, 551, 702
BseNI	ACTGG	1 cut(s)	685
BseRI	GAGGAG	7 cut(s)	48, 257, 347, 410, 440, 646, 659
BseXI	GCAGC	2 cut(s)	400, 472
BsgI	GTGCAG	1 cut(s)	374
Bsh1236I	CGCG	3 cut(s)	59, 469, 539
Bsh1285I	CGRYCG	1 cut(s)	473
BshFI	GGCC	2 cut(s)	41, 67
BsiEI	CGRYCG	1 cut(s)	473
BsiHKAI	GWGCWC	2 cut(s)	183, 246
BsiSI	CCGG	2 cut(s)	301, 343
BslFI	GGGAC	1 cut(s)	151
BslI	CCNNNNNNNGG	1 cut(s)	592
BsmAI	GTCTC	1 cut(s)	470
BsmBI	CGTCTC	1 cut(s)	470
BsmFI	GGGAC	1 cut(s)	151
BsnI	GGCC	2 cut(s)	41, 67
Bsp1286I	GDGCHC	3 cut(s)	183, 246, 689
Bsp143I	GATC	3 cut(s)	405, 438, 486
BspACI	CCGC	8 cut(s)	47, 263, 464, 467, 469, 515, 527, 537
BspANI	GGCC	2 cut(s)	41, 67
BspCNI	CTCAG	3 cut(s)	318, 552, 701
BspFNI	CGCG	3 cut(s)	59, 469, 539
BspQI	GCTCTTC	1 cut(s)	618
BsrBI	CCGCTC	1 cut(s)	265
BsrFI	RCCGGY	1 cut(s)	342
BsrI	ACTGG	1 cut(s)	685
BssAI	RCCGGY	1 cut(s)	342
BssECI	CCNNGG	3 cut(s)	42, 225, 467
BssMI	GATC	3 cut(s)	405, 438, 486
BssNI	GRCGYC	1 cut(s)	81
BssSI	CACGAG	1 cut(s)	121
BssT1I	CCWWGG	1 cut(s)	225
Bst2BI	CACGAG	1 cut(s)	121
Bst2UI	CCWGG	1 cut(s)	239
Bst6I	CTCTTC	1 cut(s)	618
BstACI	GRCGYC	1 cut(s)	81
BstAPI	GCANNNNNTGC	2 cut(s)	352, 524
BstC8I	GCNNGC	2 cut(s)	222, 357
BstDEI	CTNAG	3 cut(s)	326, 560, 688
BstDSI	CCRYGG	2 cut(s)	42, 467
BstF5I	GGATG	4 cut(s)	295, 529, 604, 679
BstFNI	CGCG	3 cut(s)	59, 469, 539
BstKTI	GATC	3 cut(s)	408, 441, 489
BstMAI	GTCTC	1 cut(s)	470
BstMBI	GATC	3 cut(s)	405, 438, 486
BstMCI	CGRYCG	1 cut(s)	473
BstMWI	GCNNNNNNNGC	6 cut(s)	47, 56, 352, 385, 466, 524
BstNI	CCWGG	1 cut(s)	239
BstNSI	RCATGY	2 cut(s)	593, 704
BstSCI	CCNGG	2 cut(s)	237, 300
BstUI	CGCG	3 cut(s)	59, 469, 539
BstV1I	GCAGC	2 cut(s)	400, 472
BstV2I	GAAGAC	1 cut(s)	368
BsuRI	GGCC	2 cut(s)	41, 67
BtgI	CCRYGG	2 cut(s)	42, 467
BtrI	CACGTC	1 cut(s)	165
BtsCI	GGATG	4 cut(s)	295, 529, 604, 679
Cac8I	GCNNGC	2 cut(s)	222, 357
Cfr10I	RCCGGY	1 cut(s)	342
Cfr13I	GGNCC	2 cut(s)	65, 111
Cfr42I	CCGCGG	1 cut(s)	470
CseI	GACGC	2 cut(s)	487, 559
CviAII	CATG	2 cut(s)	590, 701
DdeI	CTNAG	3 cut(s)	326, 560, 688
DpnI	GATC	3 cut(s)	407, 440, 488
DpnII	GATC	3 cut(s)	405, 438, 486
Eam1104I	CTCTTC	1 cut(s)	618
EarI	CTCTTC	1 cut(s)	618
Ecl136II	GAGCTC	2 cut(s)	181, 244
Eco130I	CCWWGG	1 cut(s)	225
Eco24I	GRGCYC	3 cut(s)	183, 246, 689
Eco47I	GGWCC	1 cut(s)	111
Eco53kI	GAGCTC	2 cut(s)	181, 244
EcoICRI	GAGCTC	2 cut(s)	181, 244
EcoRII	CCWGG	1 cut(s)	237
EcoT14I	CCWWGG	1 cut(s)	225
EcoT22I	ATGCAT	1 cut(s)	702
EcoT38I	GRGCYC	3 cut(s)	183, 246, 689
ErhI	CCWWGG	1 cut(s)	225
Esp3I	CGTCTC	1 cut(s)	470
FaeI	CATG	2 cut(s)	593, 704
FaiI	YATR	4 cut(s)	491, 591, 617, 702
FaqI	GGGAC	1 cut(s)	151
FatI	CATG	2 cut(s)	589, 700
Fnu4HI	GCNGC	7 cut(s)	5, 48, 389, 461, 464, 467, 516
FokI	GGATG	4 cut(s)	302, 536, 611, 686
FriOI	GRGCYC	3 cut(s)	183, 246, 689
Fsp4HI	GCNGC	7 cut(s)	5, 48, 389, 461, 464, 467, 516
FspBI	CTAG	2 cut(s)	392, 709
GluI	GCNGC	7 cut(s)	5, 48, 389, 461, 464, 467, 516
GsuI	CTGGAG	3 cut(s)	134, 221, 575
HaeIII	GGCC	2 cut(s)	41, 67
HapII	CCGG	2 cut(s)	301, 343
HgaI	GACGC	2 cut(s)	487, 559
Hin1I	GRCGYC	1 cut(s)	81
Hin1II	CATG	2 cut(s)	593, 704
HincII	GTYRAC	1 cut(s)	706
HindII	GTYRAC	1 cut(s)	706
HinfI	GANTC	3 cut(s)	259, 295, 434
HpaII	CCGG	2 cut(s)	301, 343
Hpy166II	GTNNAC	3 cut(s)	162, 545, 706
Hpy188I	TCNGA	5 cut(s)	111, 443, 475, 496, 691
Hpy188III	TCNNGA	5 cut(s)	151, 256, 271, 382, 484
Hpy8I	GTNNAC	3 cut(s)	162, 545, 706
Hpy99I	CGWCG	2 cut(s)	179, 287
HpyAV	CCTTC	2 cut(s)	408, 499
HpyCH4IV	ACGT	3 cut(s)	81, 164, 251
HpyCH4V	TGCA	5 cut(s)	355, 589, 607, 677, 700
HpyF10VI	GCNNNNNNNGC	6 cut(s)	47, 56, 352, 385, 466, 524
HpyF3I	CTNAG	3 cut(s)	326, 560, 688
HpySE526I	ACGT	3 cut(s)	81, 164, 251
Hsp92I	GRCGYC	1 cut(s)	81
Hsp92II	CATG	2 cut(s)	593, 704
KspI	CCGCGG	1 cut(s)	470
Kzo9I	GATC	3 cut(s)	405, 438, 486
LguI	GCTCTTC	1 cut(s)	618
LmnI	GCTCC	5 cut(s)	153, 241, 270, 556, 588
Lsp1109I	GCAGC	2 cut(s)	400, 472
LweI	GCATC	4 cut(s)	280, 366, 514, 664
MaeI	CTAG	2 cut(s)	392, 709
MaeII	ACGT	3 cut(s)	81, 164, 251
MalI	GATC	3 cut(s)	407, 440, 488
MbiI	CCGCTC	1 cut(s)	265
MboI	GATC	3 cut(s)	405, 438, 486
MboII	GAAGA	7 cut(s)	27, 86, 154, 166, 373, 577, 635
MhlI	GDGCHC	3 cut(s)	183, 246, 689
MluCI	AATT	3 cut(s)	313, 446, 639
MluI	ACGCGT	1 cut(s)	57
MlyI	GAGTC	1 cut(s)	443
MmeI	TCCRAC	1 cut(s)	453
Mph1103I	ATGCAT	1 cut(s)	702
MroXI	GAANNNNTTC	2 cut(s)	22, 158
MseI	TTAA	2 cut(s)	339, 638
MslI	CAYNNNNRTG	1 cut(s)	374
MspA1I	CMGCKG	1 cut(s)	469
MspI	CCGG	2 cut(s)	301, 343
MspR9I	CCNGG	2 cut(s)	239, 302
MvaI	CCWGG	1 cut(s)	239
MvnI	CGCG	3 cut(s)	59, 469, 539
MwoI	GCNNNNNNNGC	6 cut(s)	47, 56, 352, 385, 466, 524
NciI	CCSGG	1 cut(s)	302
NdeII	GATC	3 cut(s)	405, 438, 486
NlaIII	CATG	2 cut(s)	593, 704
NsiI	ATGCAT	1 cut(s)	702
NspI	RCATGY	2 cut(s)	593, 704
PciSI	GCTCTTC	1 cut(s)	618
PcsI	WCGNNNNNNNCGW	5 cut(s)	69, 78, 180, 189, 210
PdmI	GAANNNNTTC	2 cut(s)	22, 158
PfeI	GAWTC	2 cut(s)	259, 295
PfoI	TCCNGGA	2 cut(s)	237, 300
PkrI	GCNGC	7 cut(s)	6, 49, 390, 462, 465, 468, 517
PleI	GAGTC	1 cut(s)	442
PpsI	GAGTC	1 cut(s)	442
Psp124BI	GAGCTC	2 cut(s)	183, 246
Psp6I	CCWGG	1 cut(s)	237
PspGI	CCWGG	1 cut(s)	237
PspPI	GGNCC	2 cut(s)	65, 111
RseI	CAYNNNNRTG	1 cut(s)	374
SacI	GAGCTC	2 cut(s)	183, 246
SacII	CCGCGG	1 cut(s)	470
SapI	GCTCTTC	1 cut(s)	618
SaqAI	TTAA	2 cut(s)	339, 638
SatI	GCNGC	7 cut(s)	5, 48, 389, 461, 464, 467, 516
Sau3AI	GATC	3 cut(s)	405, 438, 486
Sau96I	GGNCC	2 cut(s)	65, 111
SchI	GAGTC	1 cut(s)	443
ScrFI	CCNGG	2 cut(s)	239, 302
SduI	GDGCHC	3 cut(s)	183, 246, 689
SfaNI	GCATC	4 cut(s)	280, 366, 514, 664
Sfr303I	CCGCGG	1 cut(s)	470
SgrBI	CCGCGG	1 cut(s)	470
SinI	GGWCC	1 cut(s)	111
SmiMI	CAYNNNNRTG	1 cut(s)	374
SmlI	CTYRAG	3 cut(s)	269, 380, 482
SmoI	CTYRAG	3 cut(s)	269, 380, 482
Sse9I	AATT	3 cut(s)	313, 446, 639
SsiI	CCGC	8 cut(s)	47, 263, 464, 467, 469, 515, 527, 537
SspMI	CTAG	2 cut(s)	392, 709
SstI	GAGCTC	2 cut(s)	183, 246
StyD4I	CCNGG	2 cut(s)	237, 300
StyI	CCWWGG	1 cut(s)	225
TaiI	ACGT	3 cut(s)	84, 167, 254
TaqI	TCGA	6 cut(s)	72, 177, 183, 255, 408, 437
TaqII	GACCGA	1 cut(s)	380
TasI	AATT	3 cut(s)	313, 446, 639
TauI	GCSGC	4 cut(s)	50, 466, 469, 518
TfiI	GAWTC	2 cut(s)	259, 295
Tru1I	TTAA	2 cut(s)	339, 638
Tru9I	TTAA	2 cut(s)	339, 638
TseI	GCWGC	3 cut(s)	4, 388, 460
TspGWI	ACGGA	1 cut(s)	562
VpaK11BI	GGWCC	1 cut(s)	111
XceI	RCATGY	2 cut(s)	593, 704
XmnI	GAANNNNTTC	2 cut(s)	22, 158
XspI	CTAG	2 cut(s)	392, 709
ZraI	GACGTC	1 cut(s)	82
Zsp2I	ATGCAT	1 cut(s)	702

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.