Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr05

Orthogroup ID

OG0003857

Physical Location & Seq

Genomic Coordinates Reverse (-)

5382317 .. 5394312

Gene Structure 11996 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD05G1033400.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3510 bp

ATGGCTGCTACATCTGAAAGATGGTTTGATCGGCTCCAGTTCTCCTCATTGTTTGGGCCGCCCCCGCCGGACAACCTACGACGGAAGGCAGAAATTACTGCATATGTGGACTACTTTGGTCAGTTTACATCAGAACAGTTTCCTGAGGATGTTGCCGAACTGATCCGCAACCGTTATCCATCAGAGGTGAAGCGTCTCTTTGATGATGTTCTAGCTATGTTTGTTCTTCATCACCCTGAGCATGGGCATGCTGTTATCCTTCCAATTATTTCATGCATCATTGATGGTACCCTGGCCTATAAGAGCAGTGGCCCTCCATTTGCTTCGTTCATATCATTGGTCTGCCCAAGCAGTGAGGAGTATTCTGAGCAGTGGGCTTTGGCATGTGGGGAGATTTTGCGAATTTTAACTCATTACAATCGCCCTATATACAAGGCGGAACAACAGAATAATGAAACAGAAAGAAGCAGCAGTGGAAGCCATGCCACAACAAGTGACAATGTTGATGGTGAATCCAGCCATTTACCTTTGGTACAACAAGAGAAGAAACCCATTAGGCCATTGTCTCCTTGGATTACTGATATATTGCTTGCTGCACCTCTAGGCATCAGAAGTGATTACTTCCGCTGGTGCAGTGGGGTTATGGGAAAATATGCTGCTGGAGAACTCAAGCCACCATCAACTGCTTCTTCTCGTGGATCTGGAAAACATCCACAGCTCATGCCATCAACTCCAAGGTGGGCTGTTGCAAACGGTGCTGGAGTTATATTAAGTGTTTGTGACGAGGAAGTTGCTCGCTACGAGACTGCTACTTTGACAGCAGTCGCTGTCCCTGCACTTCTTCTTCCTCCTCCAACTACAGCTTTGGATGAACACCTAGTGGCAGGGCTACCAGCTCTTGAGCCTTATGCACGTTTATTTCATAGGTATTATGCCATTGCTACTCCAAGTGCCACCCAAAGACTTCTTCTTGGACTTCTAGAAGCACCACCATCCTGGGCTCCTGATGCACTTGATGCGGCTGTACAGCTTGTGGAACTACTTCGTGCTGCTGAGGATTATGCATCTGGCATAAGGCTTCCTAGGAACTGGATGCATTTACATTTCTTGCGGGCAATTGGGACTGCAATGTCCATGCGAGCAGGTATAGCAGCTGATGCTGCAGCAGCTTTACTTTTCCGAATACTCTCACAGCCTGCATTGCTTTTCCCTCCACTAAGACAAGTTGATGGAGTTGAAGTTCAGCATGAACCTTTGGGTGGTTACATCTCAAGCTACAAGAAACAGATCGAACTTCCTGCTGCGGAAGCAACAATTGAAGCTACTGCCCAAGGGATTGCTTCTATGCTCTGTGCCCATGGGCCTGAGGTTGAATGGAGAATTTGTACCATATGGGAAGCTGCCTACGGCCTCATTCCTTTAAGTTCCTCTGCAGTTGACCTTCCTGAGATTATTGTTGCCACTCCATTACAACCTCCGATTTTATCATGGAATCTGTATATACCGCTCCTCAAGGTCTTGGAGTATCTTCCTCGTGGAAGTCCTTCTGAGGCATGTCTTATGAAGATATTTGTTGCCACTGTTGAAGCGATTCTTCAGAGAACATTTCCGTCCGAGTCCTCTAGAGAGCAAAACCGGAAAACAAGGTACCTTTTTGGTATGGGGCCAGCCTCAAAAAACCTGGCTGTTGCAGAGCTGCGTACAATGGTTCATTCACTATTCTTAGAATCATGTGCCTCAGTAGAGCTTGCTTCACGTCTACTTTTTGTTGTGTTAACTGTATGCGTCAGTCATGAAGCCCAGTCCAATGGTAGCAAGAAAGCAAGAGTTGATGAAAGCTTCCCACCAGATGAGAGCATTGAAGAATCAGAAAAGATGTCTGGTATGCAGAGAGACAGAACTAAAATGACCAAAAAACAAGGGCCAGTAGCAGCATTTGATTCTTATGTTCTGGCTGCTGTTTGTGCTCTTGCATGTGAGCTTCAGTTGTTCCCTTTGATTTCAAAGGGGATTAATCATAGTCACTCTAAAAATGCAAAAAATGTAGCGAAGCCTGCCAAAGTAAATGTATGCACTACTGAGTTTCGAAGTAGTGTAGACTCGGCAGTTTGTCACACACGCAGAATATTATCAATCTTGGAAGCACTTTTTTTGCTGAAGCCATCTACAATTGGCACTTCGTGGAGTTACAGTTCAAATGAGATAGTTGCAGCAGCTATGGTTGCTGCTCATGTTTCTGAGTTATTTAGATGGTCGAAAGCTTGCATGCATGCGCTGTCTGTCTTAATGCGCTGTAAGTGGGACACTGAAATTTGCTCCAGGGCATCATCACTGTACAACCTTATTGATTTCCACAGCAAGGCTGTTGCATCTATTGTTAACAAGGCTGAACCGTTGGAAGCACGCTTAATGCAAGTTCCAATTTGGAGGGATTCCCTTGTGTGTTTTGAAGGCAGAAAACTCACTCAAGGTGGAAACAGTAGATGCACTATTGTCGGGCAGCCATCTGCCTTGCATTGTGAGGATTTGTCACATTCAGAATCTAAACAGAAATCTGTTGGTGTGTCACGTTCAAATGAGGGGTCTGGAAATACCTTTGGTAAAGGTGTTGCAAGTTTCCCATTAGATGCTTCGGATTTGGCCAACTTTCTTACCATGGACAGGCATATAGGATTTAATTGCAGTGCACAGGTTCTTCTTAGATCAGTACTCGCAGAGAAACAAGAGCTCTGTTTCTCGGTTGTCTCACTGTTGTGGTACAAGTTAATTGCAGCTCCTGAAACTCAACCTTCTGCAGAAAGCACTTCTGCTCAACAAGGATGGCGGCAGGTCGTTGATGCATTATGCAATGTTGTGTCAGCAACACCAAAAAAAGCAGCCACAGCAATCGTTCTTCAGGCGGAGAGGGAACTGCAGCCTTGGATTGCCAAAGATGATGATCAAGGTCAGAAGATGTGGCGAATCAACCAGCGGATTGTGAAATTGATTGTGGAGTTGATGAGAATTCATGATAGCCCAGAGTCATTGGTAATTTTGTCAAGTGCCTCAGATCTACTTTTGCGTGCCACCGATGGGATGCTTGTTGATGGAGAAGCTTGCACCTTACCTCAACTGGAGCTACTGGAAGCAACAGCTAGAGCAATTCAGCCTATGCTAGAGTGGGGAGAATCTGGGCTGGCGGTCTCTGATGGCCTTTCAAACCTGTTAAAGTGTCGTCTGCCAGCTACCATCCGCTGCCTTTCTCATCCTAGTGCACATGTTCGTGCTCTGAGCACATCAGTTCTCCGCGATATCTTGCAAGCCAGTTCAATAAGACCCAACCCTAATCCGGTGGAGATAAATGGTATCCATGGTCCGTCGTATAAGTATTTTAACTTAGATGTCATTGACTGGCAAGCTGATATTGAAAAGTGCTTGACATGGGAAGCACATAGCAGACTCGCGACTGGAATGCATATTAAATTTCTGGATACTGCCGCAAAAGAACTGGGTTGTAGTATTTCCATATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0000302 GO:0003006 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005737 GO:0006355 GO:0006950 GO:0006979 GO:0007275 GO:0007623 GO:0008150 GO:0009266 GO:0009314 GO:0009409 GO:0009416 GO:0009628 GO:0009636 GO:0009637 GO:0009639 GO:0009791 GO:0009889 GO:0009908 GO:0010035 GO:0010218 GO:0010378 GO:0010468 GO:0010556 GO:0019219 GO:0019222 GO:0022414 GO:0031323 GO:0031326 GO:0031974 GO:0031981 GO:0032501 GO:0032502 GO:0042221 GO:0042493 GO:0042542 GO:0042752 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044464 GO:0046677 GO:0048367 GO:0048511 GO:0048518 GO:0048578 GO:0048580 GO:0048582 GO:0048583 GO:0048584 GO:0048586 GO:0048608 GO:0048731 GO:0048856 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051094 GO:0051171 GO:0051239 GO:0051240 GO:0051252 GO:0060255 GO:0061458 GO:0065007 GO:0070013 GO:0080090 GO:0080167 GO:0090567 GO:1901700 GO:1903506 GO:2000026 GO:2000028 GO:2000112 GO:2000241 GO:2000243 GO:2001141

KEGG Pathways

Metabolic & Signaling

ko04712 map04712

Pfam Domains

Protein Families

No domains found.

Protein Analysis

1170

Amino Acids

128.21

Weight (kDa)

6.26

Isoelectric Point (pI)

52.16

Instability Index

Protein Domains (Pfam)

No Pfam domains detected for this protein.

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0003857)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G22770
fragaria_vesca	FvH4_2g13030 FvH4_2g13030 FvH4_2g13030 FvH4_2g13030 FvH4_2g13030
malus_domestica	MD05G1033400.v1.1 MD10G1034200.v1.1
prunus_persica	Prupe.8G040600_v2.0.a1 Prupe.8G040600_v2.0.a1 Prupe.8G040600_v2.0.a1 Prupe.8G040600_v2.0.a1 Prupe.8G040600_v2.0.a1 Prupe.8G040600_v2.0.a1
pyrus_communis	pycom05g02420 pycom10g02270
rosa_chinensis	RchiOBHm_Chr6g0274251
rosa_laevigata	RLG00000013569
rosa_multiflora	Rmu_sc0005132.1_g000012 Rmu_sc0007258.1_g000001 Rmu_sc0041613.1_g000001 Rmu_sc0041918.1_g000001
rosa_roxburghii	Rroxscaffold_7G00194400
rosa_rugosa	Rorug06G0080200 Rorug06G0080200 Rorug06G0080200 Rorug06G0080200 Rorug06G0080300 Rorug06G0080400
rosa_samantha	Rh6AG195200 Rh6BG199600 Rh6CG199700 Rh6DG191100
rosa_wichuraiana	Rw6G017080

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	1129
Acc36I	ACCTGC	2 cut(s)	1133, 2818
Acc65I	GGTACC	2 cut(s)	287, 1647
AccB1I	GGYRCC	2 cut(s)	287, 1647
AccBSI	CCGCTC	1 cut(s)	1507
AccI	GTMKAC	2 cut(s)	1759, 2097
AccII	CGCG	2 cut(s)	3288, 3443
AclWI	GGATC	2 cut(s)	157, 706
AcoI	YGGCCR	1 cut(s)	2640
AcsI	RAATTY	5 cut(s)	402, 1380, 2310, 3003, 3461
AcuI	CTGAAG	4 cut(s)	1580, 1967, 2177, 2879
AdeI	CACNNNGTG	2 cut(s)	880, 2181
AfaI	GTAC	9 cut(s)	289, 534, 1026, 1387, 1649, 1702, 2336, 2709, 2759
AfiI	CCNNNNNNNGG	6 cut(s)	242, 1259, 2359, 2661, 3072, 3328
AflIII	ACRYGT	1 cut(s)	3256
AjiI	CACGTC	1 cut(s)	1757
AjnI	CCWGG	4 cut(s)	291, 995, 1680, 2318
AjuI	GAANNNNNNNTTGG	2 cut(s)	951, 983
AleI	CACNNNNGTG	1 cut(s)	2752
AloI	GAACNNNNNNTCC	4 cut(s)	1224, 1256, 2176, 2208
Alw21I	GWGCWC	6 cut(s)	1969, 2689, 2730, 3256, 3268, 3275
Alw26I	GTCTC	6 cut(s)	200, 570, 797, 1887, 2749, 3187
Alw44I	GTGCAC	2 cut(s)	2685, 3252
AlwI	GGATC	2 cut(s)	157, 706
AlwNI	CAGNNNCTG	2 cut(s)	827, 2777
ApaLI	GTGCAC	2 cut(s)	2685, 3252
ApoI	RAATTY	5 cut(s)	402, 1380, 2310, 3003, 3461
ArsI	GACNNNNNNTTYG	4 cut(s)	796, 828, 2567, 2599
AseI	ATTAAT	1 cut(s)	2013
Asp700I	GAANNNNTTC	4 cut(s)	138, 1041, 1543, 1590
Asp718I	GGTACC	2 cut(s)	287, 1647
AspA2I	CCTAGG	1 cut(s)	1082
AspLEI	GCGC	2 cut(s)	2275, 2292
AspS9I	GGNCC	6 cut(s)	56, 311, 1361, 1664, 1922, 3353
AsuHPI	GGTGA	3 cut(s)	199, 224, 521
AsuII	TTCGAA	1 cut(s)	2086
AvaII	GGWCC	1 cut(s)	3353
AvrII	CCTAGG	1 cut(s)	1082
AxyI	CCTNAGG	2 cut(s)	144, 1365
BaeGI	GKGCMC	3 cut(s)	1357, 2689, 3256
BalI	TGGCCA	1 cut(s)	2642
BanI	GGYRCC	2 cut(s)	287, 1647
BanII	GRGCYC	2 cut(s)	1003, 2730
BauI	CACGAG	2 cut(s)	693, 1533
Bbv12I	GWGCWC	6 cut(s)	1969, 2689, 2730, 3256, 3268, 3275
BbvCI	CCTCAGC	1 cut(s)	1053
BceAI	ACGGC	1 cut(s)	1423
BcgI	CGANNNNNNTGC	8 cut(s)	773, 807, 1280, 1314, 2476, 2510, 3433, 3467
BciT130I	CCWGG	4 cut(s)	293, 997, 1682, 2320
BciVI	GTATCC	2 cut(s)	3356, 3463
BclI	TGATCA	1 cut(s)	2938
BcoDI	GTCTC	6 cut(s)	200, 570, 797, 1887, 2749, 3187
BfaI	CTAG	9 cut(s)	212, 602, 878, 980, 1083, 1623, 3135, 3155, 3249
BfmI	CTRYAG	5 cut(s)	858, 1161, 1431, 2793, 2912
BfuAI	ACCTGC	2 cut(s)	1133, 2818
BfuI	GTATCC	2 cut(s)	3356, 3463
BglII	AGATCT	1 cut(s)	3049
BlnI	CCTAGG	1 cut(s)	1082
BmcAI	AGTACT	1 cut(s)	2709
Bme1390I	CCNGG	4 cut(s)	293, 997, 1682, 2320
Bme18I	GGWCC	1 cut(s)	3353
BmgBI	CACGTC	1 cut(s)	1757
BmgT120I	GGNCC	6 cut(s)	56, 311, 1361, 1664, 1922, 3353
BmiI	GGNNCC	5 cut(s)	35, 289, 1002, 1649, 1665
BmrFI	CCNGG	4 cut(s)	293, 997, 1682, 2320
BmrI	ACTGGG	2 cut(s)	1795, 3497
BmuI	ACTGGG	2 cut(s)	1795, 3497
BoxI	GACNNNNGTC	1 cut(s)	821
BpmI	CTGGAG	5 cut(s)	20, 681, 780, 2302, 3134
Bpu10I	CCTNAGC	2 cut(s)	237, 1053
Bpu14I	TTCGAA	1 cut(s)	2086
BpuEI	CTTGAG	5 cut(s)	653, 920, 1255, 1496, 2451
BsaBI	GATNNNNATC	1 cut(s)	2937
BsaI	GGTCTC	1 cut(s)	3187
BsaWI	WCCGGW	2 cut(s)	1635, 3328
BsaXI	ACNNNNNCTCC	6 cut(s)	1224, 1254, 1369, 1399, 2176, 2206
Bsc4I	CCNNNNNNNGG	6 cut(s)	242, 1259, 2359, 2661, 3072, 3328
Bse21I	CCTNAGG	2 cut(s)	144, 1365
Bse3DI	GCAATG	4 cut(s)	936, 1134, 1199, 2854
Bse8I	GATNNNNATC	1 cut(s)	2937
BseBI	CCWGG	4 cut(s)	293, 997, 1682, 2320
BseGI	GGATG	9 cut(s)	154, 709, 874, 992, 1098, 2825, 3081, 3228, 3244
BseJI	GATNNNNATC	1 cut(s)	2937
BseLI	CCNNNNNNNGG	6 cut(s)	242, 1259, 2359, 2661, 3072, 3328
BseMI	GCAATG	4 cut(s)	936, 1134, 1199, 2854
BseRI	GAGGAG	4 cut(s)	34, 371, 840, 1499
BseSI	GKGCMC	3 cut(s)	1357, 2689, 3256
BsgI	GTGCAG	3 cut(s)	579, 652, 819
Bsh1236I	CGCG	2 cut(s)	3288, 3443
BshNI	GGYRCC	2 cut(s)	287, 1647
BsiHKAI	GWGCWC	6 cut(s)	1969, 2689, 2730, 3256, 3268, 3275
BsiSI	CCGG	3 cut(s)	68, 1636, 3329
BslFI	GGGAC	3 cut(s)	815, 1135, 2315
BslI	CCNNNNNNNGG	6 cut(s)	242, 1259, 2359, 2661, 3072, 3328
BsmAI	GTCTC	6 cut(s)	200, 570, 797, 1887, 2749, 3187
BsmBI	CGTCTC	1 cut(s)	200
BsmFI	GGGAC	3 cut(s)	815, 1135, 2315
BsmI	GAATGC	1 cut(s)	3456
Bso31I	GGTCTC	1 cut(s)	3187
Bsp119I	TTCGAA	1 cut(s)	2086
Bsp1286I	GDGCHC	8 cut(s)	1003, 1357, 1969, 2689, 2730, 3256, 3268, 3275
Bsp1407I	TGTACA	2 cut(s)	1024, 2334
Bsp143I	GATC	7 cut(s)	28, 162, 698, 1287, 2702, 2938, 3049
Bsp19I	CCATGG	3 cut(s)	1357, 2655, 3349
Bsp68I	TCGCGA	1 cut(s)	3443
BspFNI	CGCG	2 cut(s)	3288, 3443
BspHI	TCATGA	2 cut(s)	1792, 3007
BspLI	GGNNCC	5 cut(s)	35, 289, 1002, 1649, 1665
BspMAI	CTGCAG	4 cut(s)	1165, 1435, 2797, 2916
BspMI	ACCTGC	2 cut(s)	1133, 2818
BspPI	GGATC	2 cut(s)	157, 706
BspT104I	TTCGAA	1 cut(s)	2086
BspT107I	GGYRCC	2 cut(s)	287, 1647
BspTNI	GGTCTC	1 cut(s)	3187
BsrBI	CCGCTC	1 cut(s)	1507
BsrDI	GCAATG	4 cut(s)	936, 1134, 1199, 2854
BsrGI	TGTACA	2 cut(s)	1024, 2334
BssMI	GATC	7 cut(s)	28, 162, 698, 1287, 2702, 2938, 3049
BssSI	CACGAG	2 cut(s)	693, 1533
BssT1I	CCWWGG	8 cut(s)	569, 734, 1082, 1330, 1357, 2655, 2918, 3349
Bst2BI	CACGAG	2 cut(s)	693, 1533
Bst2UI	CCWGG	4 cut(s)	293, 997, 1682, 2320
BstAPI	GCANNNNNTGC	3 cut(s)	755, 1016, 1157
BstAUI	TGTACA	2 cut(s)	1024, 2334
BstBI	TTCGAA	1 cut(s)	2086
BstDSI	CCRYGG	3 cut(s)	1357, 2655, 3349
BstF5I	GGATG	9 cut(s)	154, 709, 874, 992, 1098, 2825, 3081, 3228, 3244
BstFNI	CGCG	2 cut(s)	3288, 3443
BstHHI	GCGC	2 cut(s)	2275, 2292
BstKTI	GATC	7 cut(s)	31, 165, 701, 1290, 2705, 2941, 3052
BstMAI	GTCTC	6 cut(s)	200, 570, 797, 1887, 2749, 3187
BstMBI	GATC	7 cut(s)	28, 162, 698, 1287, 2702, 2938, 3049
BstNI	CCWGG	4 cut(s)	293, 997, 1682, 2320
BstNSI	RCATGY	7 cut(s)	251, 387, 1557, 1977, 2269, 2273, 3260
BstPAI	GACNNNNGTC	1 cut(s)	821
BstSCI	CCNGG	4 cut(s)	291, 995, 1680, 2318
BstSFI	CTRYAG	5 cut(s)	858, 1161, 1431, 2793, 2912
BstSLI	GKGCMC	3 cut(s)	1357, 2689, 3256
BstUI	CGCG	2 cut(s)	3288, 3443
BstX2I	RGATCY	2 cut(s)	698, 3049
BstXI	CCANNNNNNTGG	2 cut(s)	996, 1808
BstYI	RGATCY	2 cut(s)	698, 3049
Bsu36I	CCTNAGG	2 cut(s)	144, 1365
BsuI	GTATCC	2 cut(s)	3356, 3463
BtgI	CCRYGG	3 cut(s)	1357, 2655, 3349
BtrI	CACGTC	1 cut(s)	1757
BtsCI	GGATG	9 cut(s)	154, 709, 874, 992, 1098, 2825, 3081, 3228, 3244
BtsI	GCAGTG	6 cut(s)	313, 358, 377, 478, 640, 2689
BtuMI	TCGCGA	1 cut(s)	3443
BveI	ACCTGC	2 cut(s)	1133, 2818
CaiI	CAGNNNCTG	2 cut(s)	827, 2777
CciI	TCATGA	2 cut(s)	1792, 3007
CfoI	GCGC	2 cut(s)	2275, 2292
Cfr13I	GGNCC	6 cut(s)	56, 311, 1361, 1664, 1922, 3353
CseI	GACGC	2 cut(s)	182, 1774
Csp6I	GTAC	9 cut(s)	288, 533, 1025, 1386, 1648, 1701, 2335, 2708, 2758
CviQI	GTAC	9 cut(s)	288, 533, 1025, 1386, 1648, 1701, 2335, 2708, 2758
DpnI	GATC	7 cut(s)	30, 164, 700, 1289, 2704, 2940, 3051
DpnII	GATC	7 cut(s)	28, 162, 698, 1287, 2702, 2938, 3049
DraIII	CACNNNGTG	2 cut(s)	880, 2181
DrdI	GACNNNNNNGTC	1 cut(s)	1129
DseDI	GACNNNNNNGTC	1 cut(s)	1129
EaeI	YGGCCR	1 cut(s)	2640
EciI	GGCGGA	2 cut(s)	452, 2915
Ecl136II	GAGCTC	1 cut(s)	2728
Eco130I	CCWWGG	8 cut(s)	569, 734, 1082, 1330, 1357, 2655, 2918, 3349
Eco24I	GRGCYC	2 cut(s)	1003, 2730
Eco31I	GGTCTC	1 cut(s)	3187
Eco32I	GATATC	1 cut(s)	3292
Eco47I	GGWCC	1 cut(s)	3353
Eco53kI	GAGCTC	1 cut(s)	2728
Eco57I	CTGAAG	4 cut(s)	1580, 1967, 2177, 2879
Eco81I	CCTNAGG	2 cut(s)	144, 1365
EcoICRI	GAGCTC	1 cut(s)	2728
EcoRI	GAATTC	1 cut(s)	3003
EcoRII	CCWGG	4 cut(s)	291, 995, 1680, 2318
EcoRV	GATATC	1 cut(s)	3292
EcoT14I	CCWWGG	8 cut(s)	569, 734, 1082, 1330, 1357, 2655, 2918, 3349
EcoT22I	ATGCAT	6 cut(s)	278, 1066, 1098, 2271, 2842, 3456
EcoT38I	GRGCYC	2 cut(s)	1003, 2730
ErhI	CCWWGG	8 cut(s)	569, 734, 1082, 1330, 1357, 2655, 2918, 3349
Esp3I	CGTCTC	1 cut(s)	200
FalI	AAGNNNNNCTT	4 cut(s)	182, 214, 1578, 1610
FaqI	GGGAC	3 cut(s)	815, 1135, 2315
FauI	CCCGC	2 cut(s)	72, 1104
FauNDI	CATATG	3 cut(s)	103, 1391, 3506
FbaI	TGATCA	1 cut(s)	2938
FblI	GTMKAC	2 cut(s)	1759, 2097
FokI	GGATG	9 cut(s)	161, 696, 881, 979, 1105, 2832, 3088, 3215, 3231
FriOI	GRGCYC	2 cut(s)	1003, 2730
FspBI	CTAG	9 cut(s)	212, 602, 878, 980, 1083, 1623, 3135, 3155, 3249
GlaI	GCGC	2 cut(s)	2274, 2291
GsuI	CTGGAG	5 cut(s)	20, 681, 780, 2302, 3134
HapII	CCGG	3 cut(s)	68, 1636, 3329
HgaI	GACGC	2 cut(s)	182, 1774
HhaI	GCGC	2 cut(s)	2275, 2292
Hin6I	GCGC	2 cut(s)	2273, 2290
HinP1I	GCGC	2 cut(s)	2273, 2290
HincII	GTYRAC	3 cut(s)	1438, 1776, 2380
HindII	GTYRAC	3 cut(s)	1438, 1776, 2380
HindIII	AAGCTT	3 cut(s)	1837, 2259, 3093
HpaI	GTTAAC	2 cut(s)	1776, 2380
HpaII	CCGG	3 cut(s)	68, 1636, 3329
HphI	GGTGA	3 cut(s)	199, 224, 521
Hpy166II	GTNNAC	9 cut(s)	109, 126, 1438, 1760, 1776, 2098, 2380, 2687, 3254
Hpy8I	GTNNAC	9 cut(s)	109, 126, 1438, 1760, 1776, 2098, 2380, 2687, 3254
Hpy99I	CGWCG	2 cut(s)	84, 3361
HpyAV	CCTTC	6 cut(s)	79, 269, 1451, 1554, 2444, 2799
HpyCH4IV	ACGT	3 cut(s)	913, 1756, 2569
HpySE526I	ACGT	3 cut(s)	913, 1756, 2569
HspAI	GCGC	2 cut(s)	2273, 2290
KpnI	GGTACC	2 cut(s)	291, 1651
Ksp22I	TGATCA	1 cut(s)	2938
KspAI	GTTAAC	2 cut(s)	1776, 2380
Kzo9I	GATC	7 cut(s)	28, 162, 698, 1287, 2702, 2938, 3049
LmnI	GCTCC	6 cut(s)	39, 1006, 1512, 2321, 2779, 3115
MaeI	CTAG	9 cut(s)	212, 602, 878, 980, 1083, 1623, 3135, 3155, 3249
MaeII	ACGT	3 cut(s)	913, 1756, 2569
MaeIII	GTNAC	8 cut(s)	494, 779, 1262, 2021, 2111, 2186, 2529, 2565
MalI	GATC	7 cut(s)	30, 164, 700, 1289, 2704, 2940, 3051
MbiI	CCGCTC	1 cut(s)	1507
MboI	GATC	7 cut(s)	28, 162, 698, 1287, 2702, 2938, 3049
MfeI	CAATTG	3 cut(s)	1116, 1314, 2169
MflI	RGATCY	2 cut(s)	698, 3049
MhlI	GDGCHC	8 cut(s)	1003, 1357, 1969, 2689, 2730, 3256, 3268, 3275
MlsI	TGGCCA	1 cut(s)	2642
MluNI	TGGCCA	1 cut(s)	2642
MlyI	GAGTC	4 cut(s)	1625, 2093, 3029, 3432
MmeI	TCCRAC	2 cut(s)	878, 2376
Mox20I	TGGCCA	1 cut(s)	2642
Mph1103I	ATGCAT	6 cut(s)	278, 1066, 1098, 2271, 2842, 3456
MroXI	GAANNNNTTC	4 cut(s)	138, 1041, 1543, 1590
MscI	TGGCCA	1 cut(s)	2642
MslI	CAYNNNNRTG	4 cut(s)	246, 2752, 3249, 3261
Msp20I	TGGCCA	1 cut(s)	2642
MspA1I	CMGCKG	4 cut(s)	627, 1154, 2971, 3234
MspI	CCGG	3 cut(s)	68, 1636, 3329
MspR9I	CCNGG	4 cut(s)	293, 997, 1682, 2320
MunI	CAATTG	3 cut(s)	1116, 1314, 2169
Mva1269I	GAATGC	1 cut(s)	3456
MvaI	CCWGG	4 cut(s)	293, 997, 1682, 2320
MvnI	CGCG	2 cut(s)	3288, 3443
NcoI	CCATGG	3 cut(s)	1357, 2655, 3349
NdeI	CATATG	3 cut(s)	103, 1391, 3506
NdeII	GATC	7 cut(s)	28, 162, 698, 1287, 2702, 2938, 3049
NlaIV	GGNNCC	5 cut(s)	35, 289, 1002, 1649, 1665
NmeAIII	GCCGAG	1 cut(s)	2081
NmuCI	GTSAC	6 cut(s)	494, 779, 2021, 2111, 2529, 2565
NruI	TCGCGA	1 cut(s)	3443
NsiI	ATGCAT	6 cut(s)	278, 1066, 1098, 2271, 2842, 3456
NspI	RCATGY	7 cut(s)	251, 387, 1557, 1977, 2269, 2273, 3260
NspV	TTCGAA	1 cut(s)	2086
OliI	CACNNNNGTG	1 cut(s)	2752
PaeI	GCATGC	3 cut(s)	251, 2269, 2273
PagI	TCATGA	2 cut(s)	1792, 3007
PciI	ACATGT	1 cut(s)	3256
PctI	GAATGC	1 cut(s)	3456
PdmI	GAANNNNTTC	4 cut(s)	138, 1041, 1543, 1590
PleI	GAGTC	4 cut(s)	1624, 2093, 3028, 3432
PpsI	GAGTC	4 cut(s)	1624, 2093, 3028, 3432
PscI	ACATGT	1 cut(s)	3256
PshAI	GACNNNNGTC	1 cut(s)	821
PshBI	ATTAAT	1 cut(s)	2013
Psp124BI	GAGCTC	1 cut(s)	2730
Psp6I	CCWGG	4 cut(s)	291, 995, 1680, 2318
PspGI	CCWGG	4 cut(s)	291, 995, 1680, 2318
PspN4I	GGNNCC	5 cut(s)	35, 289, 1002, 1649, 1665
PspPI	GGNCC	6 cut(s)	56, 311, 1361, 1664, 1922, 3353
PstI	CTGCAG	4 cut(s)	1165, 1435, 2797, 2916
PstNI	CAGNNNCTG	2 cut(s)	827, 2777
PsuI	RGATCY	2 cut(s)	698, 3049
PvuII	CAGCTG	1 cut(s)	1154
RruI	TCGCGA	1 cut(s)	3443
RsaI	GTAC	9 cut(s)	289, 534, 1026, 1387, 1649, 1702, 2336, 2709, 2759
RsaNI	GTAC	9 cut(s)	288, 533, 1025, 1386, 1648, 1701, 2335, 2708, 2758
RseI	CAYNNNNRTG	4 cut(s)	246, 2752, 3249, 3261
SacI	GAGCTC	1 cut(s)	2730
Sau3AI	GATC	7 cut(s)	28, 162, 698, 1287, 2702, 2938, 3049
Sau96I	GGNCC	6 cut(s)	56, 311, 1361, 1664, 1922, 3353
ScaI	AGTACT	1 cut(s)	2709
SchI	GAGTC	4 cut(s)	1625, 2093, 3029, 3432
ScrFI	CCNGG	4 cut(s)	293, 997, 1682, 2320
SduI	GDGCHC	8 cut(s)	1003, 1357, 1969, 2689, 2730, 3256, 3268, 3275
SfcI	CTRYAG	5 cut(s)	858, 1161, 1431, 2793, 2912
SfuI	TTCGAA	1 cut(s)	2086
SinI	GGWCC	1 cut(s)	3353
SmiMI	CAYNNNNRTG	4 cut(s)	246, 2752, 3249, 3261
SmlI	CTYRAG	5 cut(s)	668, 899, 1270, 1511, 2466
SmoI	CTYRAG	5 cut(s)	668, 899, 1270, 1511, 2466
SphI	GCATGC	3 cut(s)	251, 2269, 2273
SspI	AATATT	1 cut(s)	2127
SspMI	CTAG	9 cut(s)	212, 602, 878, 980, 1083, 1623, 3135, 3155, 3249
SstI	GAGCTC	1 cut(s)	2730
StyD4I	CCNGG	4 cut(s)	291, 995, 1680, 2318
StyI	CCWWGG	8 cut(s)	569, 734, 1082, 1330, 1357, 2655, 2918, 3349
TaiI	ACGT	3 cut(s)	916, 1759, 2572
TaqI	TCGA	3 cut(s)	1290, 2086, 2255
TatI	WGTACW	3 cut(s)	1024, 2334, 2707
TauI	GCSGC	4 cut(s)	61, 1022, 2827, 3479
TseFI	GTSAC	6 cut(s)	494, 779, 2021, 2111, 2529, 2565
Tsp45I	GTSAC	6 cut(s)	494, 779, 2021, 2111, 2529, 2565
TspGWI	ACGGA	3 cut(s)	97, 1599, 3345
VneI	GTGCAC	2 cut(s)	2685, 3252
VpaK11BI	GGWCC	1 cut(s)	3353
VspI	ATTAAT	1 cut(s)	2013
XapI	RAATTY	5 cut(s)	402, 1380, 2310, 3003, 3461
XbaI	TCTAGA	2 cut(s)	979, 1622
XceI	RCATGY	7 cut(s)	251, 387, 1557, 1977, 2269, 2273, 3260
XcmI	CCANNNNNNNNNTGG	1 cut(s)	567
XmaJI	CCTAGG	1 cut(s)	1082
XmiI	GTMKAC	2 cut(s)	1759, 2097
XmnI	GAANNNNTTC	4 cut(s)	138, 1041, 1543, 1590
XspI	CTAG	9 cut(s)	212, 602, 878, 980, 1083, 1623, 3135, 3155, 3249
ZrmI	AGTACT	1 cut(s)	2709
Zsp2I	ATGCAT	6 cut(s)	278, 1066, 1098, 2271, 2842, 3456

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.