Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr10

Orthogroup ID

OG0003857

Physical Location & Seq

Genomic Coordinates Reverse (-)

4402658 .. 4412305

Gene Structure 9648 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD10G1034200.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3513 bp

ATGGCTGCTACATCTGAAAGATGGTTTGATCGGCTGCAGTTCTCCTCATTGTTTGGGCCGCCCCCGCAGGACGCCCTACGACGGAAGGCACAAATTACTGCGTATGTGGACTACTTTGGTCAGTTTACATCAGAACAGTTTCCTGAGGATATTGCTGAGCTGATCCGCAACCGTTATCCATCAGAGGTGAAGCGTCTCTTTGATGATGTTCTAGCTATGTTTGTCCTTCATCACCCTGAGCATGGGCATGCTGTTATCCTTCCAATTATTTCATGCATCATTGATGGTACCCTGGCGTATAAGAGCAGTAGCCCTCCGTTTGCTTCGTTCATATCATTAGTCTGCCCAAGCAGTGAGAAAGAGTATTCTGAGCAGTGGGCTTTGGCATGTGGGGAGATTTTGCGAATTTTAACTCATTACAATCGGCCCATATACAAGGTGGAACAACAGAATTGTGAAACAGAAAGAAGCAGTAGTGGAAGCCATGCCACAACAAGTGACTCAGTTGATGGTGAATCCAGCCGTATACCTTTGGTACAACAAGAGAAGAAACCCATAAGGCCTTTGTCTCCTTGGATTACTGATATATTGCTTGCTGCACCTCTTGGCATCAGAAGTGATTACTTCCGCTGGTGCAGTGGGGTTATGGGAAAATATGCTGCTGGAGAGCTCAAGCCGCCATCAACTGCTTCTTCTCGTGGATCTGGAAAACATCCGCAGCTCATGCCATCAACTCCAAGGTGGGCTGTTGCAAACGGTGCTGGAGTTATATTAAGTGTTTGTGACGAGGAAGTTGCTCGCTATGAGACTGCTACTTTGACAGCAGTCGCTGTCCCTGCACTTCTTCTTCCTCCTCCAACTACAGCTTTGGATGAACATCTAGTGGCAGGGCTACCAGCTCTTGAGCCATATGCACGCTTATTTCATAGGTATTATGCCATTGCTACTCCAAGTGCCACTCAAAGACTTCTTCTTGGACTTCTAGAAGCACCACCATCCTGGGCTCCTGATGCACTTGATGCAGCTGTACAGTTAGTGGAACTCCTTCGTGCTGCCGAGGATTATGCATCTGGCATAAGGCTTCCTAGGAACTGGATGCATTTGCATTTCTTGCGGGCAATTGGGACTGCAATGTCCATGAGAGCAGGTATAGCAGCTGATGCTGCAGCAGCTTTACTTTTCCGAATACTCTCACAGCCTGCATTGCTTTTCCCTCCACTAAGACAAGTTGATGGAGTTGAAGTTCAGCATGAGCCCTTGGGTGGTTACATCTCAAGCTACAAGAAACAGATTGAACTTCCTGCGGCGGAAGCAACAATTGAAGCTACTGCCCAAGGGATTGCTTCTATGCTTTGTGCCCATGGGCCTGATGTTGAATGGCGAATTTGTACCATATGGGAAGCTGCCTATGGCCTCATTCCTTTAAGTTCCTCTGCAGTTGACCTTCCTGAGATTATTGTTGCCACTCCATTACAACCTCCAATTTTATCGTGGAATCTGTATATACCCCTCCTTAAGGTCTTGGAGTATCTTCCTCGTGGAAGTCCTTCTGAGGCATGTCTTATGAAGATATTTGTTGCCACTGTTGAAGCGATTCTTCAGAGAACATTTCCGTCTGAGTCCTCTAGAGAGCAAAACCGGAAAACAAGGTACCTTTTTGGCTTGGGGTCGGCCTCAAAAAACCTGGCTGTGGCAGAGCTGCGTACAATGGTTCATTCACTATTCTTAGAATCCTGTGCCTCAGTAGAGCTTGCTTCACGTTTACTTTTTGTTGTGTTAACCGTATGTGTCAGTCATGAAGCACAGTCAAATGGTAGCAAGAAAGCAAGAGTTGATGAAAGTTTCCCACCAGATGAGAGCATTGAAGAATCAGAAAAGATGTCTGATAAGCAGAGAGACAGAACTAAAAAGACCAAAAAACAAGGGCCAGTAGCAGCATTTGATTCTTATGTTCTGGCTGCTGTTTGTGCTCTTGCATGTGAGCTTCAGTTGTTCCCTTTGATTTCAAAGGGGACTAATCATGCTCACTCTAAAAATGGAAAAAATGTAGCGAAGCCTGCCAAAGTAAATGTATGCACTAATGAGTTTCAAAGTAGTGTAGACTCAGCAGTTTGTCACACACGCAGAATATTATCGATCTTGGAGGCACTTTTTTTGCTGAAGCCATCTACTATTGGCACTTCATGGAGTTACAGTTCAAATGAGATAGTTGCAGCAGCTATGGTTGCCGCTCATGTTTCTGAGTTATTTAGATGGTCGAAAGCTTGCATGCATGCGCTGTCTGTCTTGATGCGCTGTAAGTGGGACAGTGAAATTTCCTCCAGGGCAGCATCACTATACAACCTTATTGATTTCCACAGCAAGGCTGTTGCATCTATAGTTAACAAGGCTGAACCATTGGAAGCACACTTAATGCAAGTTCCAATTTGGAGGGATTCCTTCGTGTGTTTTGAAGGCAGAAAACTCAGTCAAGGTGGAAACAGTAGATGCTCTAATGTAGGTCAGCCATCTGCCTTGCAATGTGAGGATTCTTCACATTCAGAATCTAAACGCAAATCTGATATTGCATCATGTTCAAATGAGGGGTCCGGAAATACTATTGGTAAAGGTGTTGCAAGTTTCCCATTAGATGCTTCGGATTTGGCCAACTTTCTTACTAAGGACAGGCATATAGGATTTAGTTGCAGTGCACAGGTTCTTCTTAGATCCGTACTCACAGAGAAACAAGAGCTGTGTTTCTCAGTTGTCTCACTGTTGTGGTACAAGTTAATTGCAGCTCCTGAAACCCAACCTTCTGCAGAAAGTACTTCTGCTCAACAAGGATGGCGGCAGGTTGTTGATGCATTATGCAATGTTGTCTCAGCAACACCAAAAAAAGCAGCCACAGCAGTTGTTCTTCAGGCGGAGAGGGAACTGCAACCTTGGATTGCCAAAGATGATGATCAAGGTCAGAAGATGTGGAGAATCAACCAGCGAATTGTGAAATTGATTGTGGAGTTAATGAGAATTCATGATAGCCCGGAGTCATTGGTAATTTTGTCAAGTGCCTCAGATCTACTTTTGCGTGCCACCGATGGGATGCTTGTTGATGGAGAAGCTTGCACCTTGCCCCAACTGGAGCTACTGGAAGCAACAGCTAGAGCAATTCAGCCTATGCTAGAGTGGGGAGAATCTGGGCTAGCAGTCGCAGATGGCCTTTCAAACCTGCTAAAGTGTCGTCTGCCAGCCACCATCCGATGCCTTTCTCATCCCAGTGCACATGTGCGTGCTCTGAGCACATCAGTTCTTCGCGATATCTTGCAAGCCAGTTCAATTAGACCTAACCCTAATCCGGTGGAGATAGATGGTATCCATGGTCCGTCCTATAAGTATTTTAACTTAGATGTCATTGACTGGCAAGGTGACATAGAAAAGTGCTTGACATGGGAAGCTCATAGCCGACTTGCGACTGGAATGCAGATTAAATTTCTTGATACTGCCGCAAAGGAATTGGGTTGTAGTATTTCCTTGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0000302 GO:0003006 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005737 GO:0006355 GO:0006950 GO:0006979 GO:0007275 GO:0007623 GO:0008150 GO:0009266 GO:0009314 GO:0009409 GO:0009416 GO:0009628 GO:0009636 GO:0009637 GO:0009639 GO:0009791 GO:0009889 GO:0009908 GO:0010035 GO:0010218 GO:0010378 GO:0010468 GO:0010556 GO:0019219 GO:0019222 GO:0022414 GO:0031323 GO:0031326 GO:0031974 GO:0031981 GO:0032501 GO:0032502 GO:0042221 GO:0042493 GO:0042542 GO:0042752 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044464 GO:0046677 GO:0048367 GO:0048511 GO:0048518 GO:0048578 GO:0048580 GO:0048582 GO:0048583 GO:0048584 GO:0048586 GO:0048608 GO:0048731 GO:0048856 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051094 GO:0051171 GO:0051239 GO:0051240 GO:0051252 GO:0060255 GO:0061458 GO:0065007 GO:0070013 GO:0080090 GO:0080167 GO:0090567 GO:1901700 GO:1903506 GO:2000026 GO:2000028 GO:2000112 GO:2000241 GO:2000243 GO:2001141

KEGG Pathways

Metabolic & Signaling

ko04712 map04712

Pfam Domains

Protein Families

No domains found.

Protein Analysis

1171

Amino Acids

128.17

Weight (kDa)

6.27

Isoelectric Point (pI)

51.67

Instability Index

Protein Domains (Pfam)

No Pfam domains detected for this protein.

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0003857)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G22770
fragaria_vesca	FvH4_2g13030 FvH4_2g13030 FvH4_2g13030 FvH4_2g13030 FvH4_2g13030
malus_domestica	MD05G1033400.v1.1 MD10G1034200.v1.1
prunus_persica	Prupe.8G040600_v2.0.a1 Prupe.8G040600_v2.0.a1 Prupe.8G040600_v2.0.a1 Prupe.8G040600_v2.0.a1 Prupe.8G040600_v2.0.a1 Prupe.8G040600_v2.0.a1
pyrus_communis	pycom05g02420 pycom10g02270
rosa_chinensis	RchiOBHm_Chr6g0274251
rosa_laevigata	RLG00000013569
rosa_multiflora	Rmu_sc0005132.1_g000012 Rmu_sc0007258.1_g000001 Rmu_sc0041613.1_g000001 Rmu_sc0041918.1_g000001
rosa_roxburghii	Rroxscaffold_7G00194400
rosa_rugosa	Rorug06G0080200 Rorug06G0080200 Rorug06G0080200 Rorug06G0080200 Rorug06G0080300 Rorug06G0080400
rosa_samantha	Rh6AG195200 Rh6BG199600 Rh6CG199700 Rh6DG191100
rosa_wichuraiana	Rw6G017080

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	1132
Acc36I	ACCTGC	3 cut(s)	1136, 2821, 3213
Acc65I	GGTACC	2 cut(s)	287, 1650
AccB1I	GGYRCC	2 cut(s)	287, 1650
AccBSI	CCGCTC	1 cut(s)	2231
AccI	GTMKAC	2 cut(s)	526, 2100
AccII	CGCG	1 cut(s)	3291
AccIII	TCCGGA	1 cut(s)	2588
AclWI	GGATC	3 cut(s)	157, 709, 2700
AcoI	YGGCCR	1 cut(s)	2643
AcsI	RAATTY	5 cut(s)	405, 1383, 2313, 3006, 3464
AcuI	CTGAAG	4 cut(s)	1583, 1970, 2180, 2882
AcyI	GRCGYC	1 cut(s)	72
AfaI	GTAC	9 cut(s)	289, 537, 1029, 1390, 1652, 1705, 2712, 2762, 2806
AfiI	CCNNNNNNNGG	8 cut(s)	81, 242, 1262, 1516, 1690, 2362, 3075, 3331
AflII	CTTAAG	1 cut(s)	1514
AflIII	ACRYGT	1 cut(s)	3259
AjnI	CCWGG	4 cut(s)	291, 998, 1683, 2321
AleI	CACNNNNGTG	1 cut(s)	2755
AloI	GAACNNNNNNTCC	4 cut(s)	1227, 1259, 2179, 2211
Alw21I	GWGCWC	6 cut(s)	672, 1972, 2692, 3259, 3271, 3278
Alw26I	GTCTC	6 cut(s)	200, 573, 800, 1890, 2752, 2863
Alw44I	GTGCAC	2 cut(s)	2688, 3255
AlwI	GGATC	3 cut(s)	157, 709, 2700
AlwNI	CAGNNNCTG	2 cut(s)	830, 2780
Aor13HI	TCCGGA	1 cut(s)	2588
AoxI	GGCC	9 cut(s)	56, 425, 560, 1364, 1411, 1671, 1925, 2643, 3193
ApaLI	GTGCAC	2 cut(s)	2688, 3255
ApoI	RAATTY	5 cut(s)	405, 1383, 2313, 3006, 3464
ArsI	GACNNNNNNTTYG	4 cut(s)	799, 831, 2570, 2602
Asp700I	GAANNNNTTC	4 cut(s)	138, 1044, 1546, 1593
Asp718I	GGTACC	2 cut(s)	287, 1650
AspA2I	CCTAGG	1 cut(s)	1085
AspLEI	GCGC	2 cut(s)	2278, 2295
AspS9I	GGNCC	6 cut(s)	56, 426, 1364, 1925, 2586, 3356
AsuC2I	CCSGG	1 cut(s)	3020
AsuHPI	GGTGA	4 cut(s)	199, 224, 524, 3413
AsuNHI	GCTAGC	1 cut(s)	3178
AvaII	GGWCC	2 cut(s)	2586, 3356
AvrII	CCTAGG	1 cut(s)	1085
AxyI	CCTNAGG	1 cut(s)	144
BaeGI	GKGCMC	3 cut(s)	1360, 2692, 3259
BalI	TGGCCA	1 cut(s)	2645
BanI	GGYRCC	2 cut(s)	287, 1650
BanII	GRGCYC	3 cut(s)	672, 1006, 1257
BauI	CACGAG	2 cut(s)	696, 1536
Bbv12I	GWGCWC	6 cut(s)	672, 1972, 2692, 3259, 3271, 3278
BceAI	ACGGC	1 cut(s)	507
BcgI	CGANNNNNNTGC	6 cut(s)	776, 810, 1046, 1080, 3436, 3470
BciT130I	CCWGG	4 cut(s)	293, 1000, 1685, 2323
BciVI	GTATCC	1 cut(s)	3359
BclI	TGATCA	1 cut(s)	2941
BcnI	CCSGG	1 cut(s)	3020
BcoDI	GTCTC	6 cut(s)	200, 573, 800, 1890, 2752, 2863
BfaI	CTAG	8 cut(s)	212, 881, 983, 1086, 1626, 3138, 3158, 3179
BfmI	CTRYAG	6 cut(s)	35, 861, 1164, 1434, 2376, 2796
BfrI	CTTAAG	1 cut(s)	1514
BfuAI	ACCTGC	3 cut(s)	1136, 2821, 3213
BfuI	GTATCC	1 cut(s)	3359
BglII	AGATCT	1 cut(s)	3052
BlnI	CCTAGG	1 cut(s)	1085
BlpI	GCTNAGC	1 cut(s)	156
BmcAI	AGTACT	1 cut(s)	2806
Bme1390I	CCNGG	5 cut(s)	293, 1000, 1685, 2323, 3020
Bme18I	GGWCC	2 cut(s)	2586, 3356
BmgT120I	GGNCC	6 cut(s)	56, 426, 1364, 1925, 2586, 3356
BmiI	GGNNCC	4 cut(s)	289, 1005, 1652, 2587
BmrFI	CCNGG	5 cut(s)	293, 1000, 1685, 2323, 3020
BmrI	ACTGGG	1 cut(s)	3246
BmtI	GCTAGC	1 cut(s)	3182
BmuI	ACTGGG	1 cut(s)	3246
BoxI	GACNNNNGTC	1 cut(s)	824
BpmI	CTGGAG	4 cut(s)	684, 783, 2305, 3137
Bpu10I	CCTNAGC	1 cut(s)	237
Bpu1102I	GCTNAGC	1 cut(s)	156
BpuEI	CTTGAG	3 cut(s)	656, 923, 1258
BpuMI	CCSGG	1 cut(s)	3020
Bsa29I	ATCGAT	1 cut(s)	2135
BsaBI	GATNNNNATC	2 cut(s)	876, 2940
BsaHI	GRCGYC	1 cut(s)	72
BsaWI	WCCGGW	3 cut(s)	1638, 2588, 3331
BsaXI	ACNNNNNCTCC	4 cut(s)	1227, 1257, 2179, 2209
Bsc4I	CCNNNNNNNGG	8 cut(s)	81, 242, 1262, 1516, 1690, 2362, 3075, 3331
Bse1I	ACTGG	8 cut(s)	1097, 1928, 3120, 3129, 3252, 3306, 3398, 3454
Bse21I	CCTNAGG	1 cut(s)	144
Bse3DI	GCAATG	5 cut(s)	939, 1137, 1202, 2525, 2857
Bse8I	GATNNNNATC	2 cut(s)	876, 2940
BseAI	TCCGGA	1 cut(s)	2588
BseBI	CCWGG	4 cut(s)	293, 1000, 1685, 2323
BseCI	ATCGAT	1 cut(s)	2135
BseGI	GGATG	8 cut(s)	712, 877, 995, 1101, 2828, 3084, 3231, 3247
BseJI	GATNNNNATC	2 cut(s)	876, 2940
BseLI	CCNNNNNNNGG	8 cut(s)	81, 242, 1262, 1516, 1690, 2362, 3075, 3331
BseMI	GCAATG	5 cut(s)	939, 1137, 1202, 2525, 2857
BseNI	ACTGG	8 cut(s)	1097, 1928, 3120, 3129, 3252, 3306, 3398, 3454
BseRI	GAGGAG	2 cut(s)	34, 843
BseSI	GKGCMC	3 cut(s)	1360, 2692, 3259
BsgI	GTGCAG	3 cut(s)	582, 655, 822
Bsh1236I	CGCG	1 cut(s)	3291
BshFI	GGCC	9 cut(s)	58, 427, 562, 1366, 1413, 1673, 1927, 2645, 3195
BshNI	GGYRCC	2 cut(s)	287, 1650
BshVI	ATCGAT	1 cut(s)	2135
BsiHKAI	GWGCWC	6 cut(s)	672, 1972, 2692, 3259, 3271, 3278
BsiSI	CCGG	4 cut(s)	1639, 2589, 3020, 3332
BslFI	GGGAC	4 cut(s)	818, 1138, 2026, 2318
BslI	CCNNNNNNNGG	8 cut(s)	81, 242, 1262, 1516, 1690, 2362, 3075, 3331
BsmAI	GTCTC	6 cut(s)	200, 573, 800, 1890, 2752, 2863
BsmBI	CGTCTC	1 cut(s)	200
BsmFI	GGGAC	4 cut(s)	818, 1138, 2026, 2318
BsmI	GAATGC	1 cut(s)	3459
BsnI	GGCC	9 cut(s)	58, 427, 562, 1366, 1413, 1673, 1927, 2645, 3195
Bsp1286I	GDGCHC	9 cut(s)	672, 1006, 1257, 1360, 1972, 2692, 3259, 3271, 3278
Bsp13I	TCCGGA	1 cut(s)	2588
Bsp1407I	TGTACA	1 cut(s)	1027
Bsp143I	GATC	7 cut(s)	28, 162, 701, 2136, 2705, 2941, 3052
Bsp1720I	GCTNAGC	1 cut(s)	156
Bsp19I	CCATGG	2 cut(s)	1360, 3352
Bsp68I	TCGCGA	1 cut(s)	3291
BspANI	GGCC	9 cut(s)	58, 427, 562, 1366, 1413, 1673, 1927, 2645, 3195
BspDI	ATCGAT	1 cut(s)	2135
BspEI	TCCGGA	1 cut(s)	2588
BspFNI	CGCG	1 cut(s)	3291
BspHI	TCATGA	2 cut(s)	1795, 3010
BspLI	GGNNCC	4 cut(s)	289, 1005, 1652, 2587
BspMAI	CTGCAG	4 cut(s)	39, 1168, 1438, 2800
BspMI	ACCTGC	3 cut(s)	1136, 2821, 3213
BspOI	GCTAGC	1 cut(s)	3182
BspPI	GGATC	3 cut(s)	157, 709, 2700
BspT107I	GGYRCC	2 cut(s)	287, 1650
BspTI	CTTAAG	1 cut(s)	1514
BsrBI	CCGCTC	1 cut(s)	2231
BsrDI	GCAATG	5 cut(s)	939, 1137, 1202, 2525, 2857
BsrGI	TGTACA	1 cut(s)	1027
BsrI	ACTGG	8 cut(s)	1097, 1928, 3120, 3129, 3252, 3306, 3398, 3454
BssMI	GATC	7 cut(s)	28, 162, 701, 2136, 2705, 2941, 3052
BssNAI	GTATAC	1 cut(s)	527
BssNI	GRCGYC	1 cut(s)	72
BssSI	CACGAG	2 cut(s)	696, 1536
BssT1I	CCWWGG	8 cut(s)	572, 737, 1085, 1257, 1333, 1360, 2921, 3352
Bst1107I	GTATAC	1 cut(s)	527
Bst2BI	CACGAG	2 cut(s)	696, 1536
Bst2UI	CCWGG	4 cut(s)	293, 1000, 1685, 2323
BstACI	GRCGYC	1 cut(s)	72
BstAFI	CTTAAG	1 cut(s)	1514
BstAPI	GCANNNNNTGC	5 cut(s)	724, 758, 1019, 1111, 1160
BstAUI	TGTACA	1 cut(s)	1027
BstDSI	CCRYGG	2 cut(s)	1360, 3352
BstENI	CCTNNNNNAGG	1 cut(s)	1514
BstF5I	GGATG	8 cut(s)	712, 877, 995, 1101, 2828, 3084, 3231, 3247
BstFNI	CGCG	1 cut(s)	3291
BstHHI	GCGC	2 cut(s)	2278, 2295
BstKTI	GATC	7 cut(s)	31, 165, 704, 2139, 2708, 2944, 3055
BstMAI	GTCTC	6 cut(s)	200, 573, 800, 1890, 2752, 2863
BstMBI	GATC	7 cut(s)	28, 162, 701, 2136, 2705, 2941, 3052
BstNI	CCWGG	4 cut(s)	293, 1000, 1685, 2323
BstNSI	RCATGY	7 cut(s)	251, 390, 1560, 1980, 2272, 2276, 3263
BstPAI	GACNNNNGTC	1 cut(s)	824
BstSCI	CCNGG	5 cut(s)	291, 998, 1683, 2321, 3018
BstSFI	CTRYAG	6 cut(s)	35, 861, 1164, 1434, 2376, 2796
BstSLI	GKGCMC	3 cut(s)	1360, 2692, 3259
BstUI	CGCG	1 cut(s)	3291
BstX2I	RGATCY	3 cut(s)	701, 2705, 3052
BstXI	CCANNNNNNTGG	1 cut(s)	999
BstYI	RGATCY	3 cut(s)	701, 2705, 3052
BstZ17I	GTATAC	1 cut(s)	527
Bsu15I	ATCGAT	1 cut(s)	2135
Bsu36I	CCTNAGG	1 cut(s)	144
BsuI	GTATCC	1 cut(s)	3359
BsuRI	GGCC	9 cut(s)	58, 427, 562, 1366, 1413, 1673, 1927, 2645, 3195
BsuTUI	ATCGAT	1 cut(s)	2135
BtgI	CCRYGG	2 cut(s)	1360, 3352
BtsCI	GGATG	8 cut(s)	712, 877, 995, 1101, 2828, 3084, 3231, 3247
BtsI	GCAGTG	4 cut(s)	358, 380, 643, 2692
BtsIMutI	CAGTG	8 cut(s)	358, 380, 643, 1581, 2314, 2692, 2750, 3259
BtuMI	TCGCGA	1 cut(s)	3291
BveI	ACCTGC	3 cut(s)	1136, 2821, 3213
CaiI	CAGNNNCTG	2 cut(s)	830, 2780
CciI	TCATGA	2 cut(s)	1795, 3010
CfoI	GCGC	2 cut(s)	2278, 2295
Cfr13I	GGNCC	6 cut(s)	56, 426, 1364, 1925, 2586, 3356
ClaI	ATCGAT	1 cut(s)	2135
CseI	GACGC	2 cut(s)	80, 182
Csp6I	GTAC	9 cut(s)	288, 536, 1028, 1389, 1651, 1704, 2711, 2761, 2805
CspCI	CAANNNNNGTGG	2 cut(s)	2872, 2907
CviQI	GTAC	9 cut(s)	288, 536, 1028, 1389, 1651, 1704, 2711, 2761, 2805
DpnI	GATC	7 cut(s)	30, 164, 703, 2138, 2707, 2943, 3054
DpnII	GATC	7 cut(s)	28, 162, 701, 2136, 2705, 2941, 3052
DrdI	GACNNNNNNGTC	1 cut(s)	1132
DseDI	GACNNNNNNGTC	1 cut(s)	1132
EaeI	YGGCCR	1 cut(s)	2643
EciI	GGCGGA	2 cut(s)	1322, 2918
Ecl136II	GAGCTC	1 cut(s)	670
Eco130I	CCWWGG	8 cut(s)	572, 737, 1085, 1257, 1333, 1360, 2921, 3352
Eco147I	AGGCCT	1 cut(s)	562
Eco24I	GRGCYC	3 cut(s)	672, 1006, 1257
Eco32I	GATATC	1 cut(s)	3295
Eco47I	GGWCC	2 cut(s)	2586, 3356
Eco53kI	GAGCTC	1 cut(s)	670
Eco57I	CTGAAG	4 cut(s)	1583, 1970, 2180, 2882
Eco81I	CCTNAGG	1 cut(s)	144
EcoICRI	GAGCTC	1 cut(s)	670
EcoNI	CCTNNNNNAGG	1 cut(s)	1514
EcoRI	GAATTC	1 cut(s)	3006
EcoRII	CCWGG	4 cut(s)	291, 998, 1683, 2321
EcoRV	GATATC	1 cut(s)	3295
EcoT14I	CCWWGG	8 cut(s)	572, 737, 1085, 1257, 1333, 1360, 2921, 3352
EcoT22I	ATGCAT	5 cut(s)	278, 1069, 1101, 2274, 2845
EcoT38I	GRGCYC	3 cut(s)	672, 1006, 1257
ErhI	CCWWGG	8 cut(s)	572, 737, 1085, 1257, 1333, 1360, 2921, 3352
Esp3I	CGTCTC	1 cut(s)	200
FalI	AAGNNNNNCTT	4 cut(s)	182, 214, 1581, 1613
FaqI	GGGAC	4 cut(s)	818, 1138, 2026, 2318
FauI	CCCGC	2 cut(s)	72, 1107
FauNDI	CATATG	2 cut(s)	910, 1394
FbaI	TGATCA	1 cut(s)	2941
FblI	GTMKAC	2 cut(s)	526, 2100
FokI	GGATG	8 cut(s)	699, 884, 982, 1108, 2835, 3091, 3218, 3234
FriOI	GRGCYC	3 cut(s)	672, 1006, 1257
FspBI	CTAG	8 cut(s)	212, 881, 983, 1086, 1626, 3138, 3158, 3179
GlaI	GCGC	2 cut(s)	2277, 2294
GsuI	CTGGAG	4 cut(s)	684, 783, 2305, 3137
HaeIII	GGCC	9 cut(s)	58, 427, 562, 1366, 1413, 1673, 1927, 2645, 3195
HapII	CCGG	4 cut(s)	1639, 2589, 3020, 3332
HgaI	GACGC	2 cut(s)	80, 182
HhaI	GCGC	2 cut(s)	2278, 2295
Hin1I	GRCGYC	1 cut(s)	72
Hin6I	GCGC	2 cut(s)	2276, 2293
HinP1I	GCGC	2 cut(s)	2276, 2293
HincII	GTYRAC	3 cut(s)	1441, 1779, 2383
HindII	GTYRAC	3 cut(s)	1441, 1779, 2383
HindIII	AAGCTT	2 cut(s)	2262, 3096
HpaI	GTTAAC	2 cut(s)	1779, 2383
HpaII	CCGG	4 cut(s)	1639, 2589, 3020, 3332
HphI	GGTGA	4 cut(s)	199, 224, 524, 3413
Hpy99I	CGWCG	1 cut(s)	84
HpyAV	CCTTC	9 cut(s)	79, 236, 269, 1055, 1454, 1557, 2447, 2449, 2802
HpyCH4IV	ACGT	1 cut(s)	1759
HpySE526I	ACGT	1 cut(s)	1759
Hsp92I	GRCGYC	1 cut(s)	72
HspAI	GCGC	2 cut(s)	2276, 2293
Kpn2I	TCCGGA	1 cut(s)	2588
KpnI	GGTACC	2 cut(s)	291, 1654
Ksp22I	TGATCA	1 cut(s)	2941
KspAI	GTTAAC	2 cut(s)	1779, 2383
Kzo9I	GATC	7 cut(s)	28, 162, 701, 2136, 2705, 2941, 3052
LmnI	GCTCC	3 cut(s)	1009, 2782, 3118
MaeI	CTAG	8 cut(s)	212, 881, 983, 1086, 1626, 3138, 3158, 3179
MaeII	ACGT	1 cut(s)	1759
MaeIII	GTNAC	6 cut(s)	497, 782, 1265, 2114, 2189, 3401
MalI	GATC	7 cut(s)	30, 164, 703, 2138, 2707, 2943, 3054
MbiI	CCGCTC	1 cut(s)	2231
MboI	GATC	7 cut(s)	28, 162, 701, 2136, 2705, 2941, 3052
MfeI	CAATTG	2 cut(s)	1119, 1317
MflI	RGATCY	3 cut(s)	701, 2705, 3052
MhlI	GDGCHC	9 cut(s)	672, 1006, 1257, 1360, 1972, 2692, 3259, 3271, 3278
MlsI	TGGCCA	1 cut(s)	2645
MluNI	TGGCCA	1 cut(s)	2645
MlyI	GAGTC	4 cut(s)	494, 1628, 2096, 3032
MmeI	TCCRAC	1 cut(s)	881
Mox20I	TGGCCA	1 cut(s)	2645
Mph1103I	ATGCAT	5 cut(s)	278, 1069, 1101, 2274, 2845
MroI	TCCGGA	1 cut(s)	2588
MroXI	GAANNNNTTC	4 cut(s)	138, 1044, 1546, 1593
MscI	TGGCCA	1 cut(s)	2645
MslI	CAYNNNNRTG	4 cut(s)	246, 2755, 3252, 3264
Msp20I	TGGCCA	1 cut(s)	2645
MspA1I	CMGCKG	3 cut(s)	630, 1025, 1157
MspCI	CTTAAG	1 cut(s)	1514
MspI	CCGG	4 cut(s)	1639, 2589, 3020, 3332
MspR9I	CCNGG	5 cut(s)	293, 1000, 1685, 2323, 3020
MunI	CAATTG	2 cut(s)	1119, 1317
Mva1269I	GAATGC	1 cut(s)	3459
MvaI	CCWGG	4 cut(s)	293, 1000, 1685, 2323
MvnI	CGCG	1 cut(s)	3291
NciI	CCSGG	1 cut(s)	3020
NcoI	CCATGG	2 cut(s)	1360, 3352
NdeI	CATATG	2 cut(s)	910, 1394
NdeII	GATC	7 cut(s)	28, 162, 701, 2136, 2705, 2941, 3052
NheI	GCTAGC	1 cut(s)	3178
NlaIV	GGNNCC	4 cut(s)	289, 1005, 1652, 2587
NmeAIII	GCCGAG	1 cut(s)	1081
NmuCI	GTSAC	4 cut(s)	497, 782, 2114, 3401
NruI	TCGCGA	1 cut(s)	3291
NsiI	ATGCAT	5 cut(s)	278, 1069, 1101, 2274, 2845
NspI	RCATGY	7 cut(s)	251, 390, 1560, 1980, 2272, 2276, 3263
OliI	CACNNNNGTG	1 cut(s)	2755
PaeI	GCATGC	3 cut(s)	251, 2272, 2276
PagI	TCATGA	2 cut(s)	1795, 3010
PceI	AGGCCT	1 cut(s)	562
PciI	ACATGT	1 cut(s)	3259
PctI	GAATGC	1 cut(s)	3459
PdmI	GAANNNNTTC	4 cut(s)	138, 1044, 1546, 1593
PleI	GAGTC	4 cut(s)	494, 1627, 2096, 3031
PpsI	GAGTC	4 cut(s)	494, 1627, 2096, 3031
PscI	ACATGT	1 cut(s)	3259
PshAI	GACNNNNGTC	1 cut(s)	824
Psp124BI	GAGCTC	1 cut(s)	672
Psp6I	CCWGG	4 cut(s)	291, 998, 1683, 2321
PspGI	CCWGG	4 cut(s)	291, 998, 1683, 2321
PspN4I	GGNNCC	4 cut(s)	289, 1005, 1652, 2587
PspPI	GGNCC	6 cut(s)	56, 426, 1364, 1925, 2586, 3356
PstI	CTGCAG	4 cut(s)	39, 1168, 1438, 2800
PstNI	CAGNNNCTG	2 cut(s)	830, 2780
PsuI	RGATCY	3 cut(s)	701, 2705, 3052
PvuII	CAGCTG	2 cut(s)	1025, 1157
RruI	TCGCGA	1 cut(s)	3291
RsaI	GTAC	9 cut(s)	289, 537, 1029, 1390, 1652, 1705, 2712, 2762, 2806
RsaNI	GTAC	9 cut(s)	288, 536, 1028, 1389, 1651, 1704, 2711, 2761, 2805
RseI	CAYNNNNRTG	4 cut(s)	246, 2755, 3252, 3264
SacI	GAGCTC	1 cut(s)	672
Sau3AI	GATC	7 cut(s)	28, 162, 701, 2136, 2705, 2941, 3052
Sau96I	GGNCC	6 cut(s)	56, 426, 1364, 1925, 2586, 3356
ScaI	AGTACT	1 cut(s)	2806
SchI	GAGTC	4 cut(s)	494, 1628, 2096, 3032
ScrFI	CCNGG	5 cut(s)	293, 1000, 1685, 2323, 3020
SduI	GDGCHC	9 cut(s)	672, 1006, 1257, 1360, 1972, 2692, 3259, 3271, 3278
SfcI	CTRYAG	6 cut(s)	35, 861, 1164, 1434, 2376, 2796
SinI	GGWCC	2 cut(s)	2586, 3356
SmiMI	CAYNNNNRTG	4 cut(s)	246, 2755, 3252, 3264
SmlI	CTYRAG	4 cut(s)	671, 902, 1273, 1514
SmoI	CTYRAG	4 cut(s)	671, 902, 1273, 1514
SphI	GCATGC	3 cut(s)	251, 2272, 2276
SseBI	AGGCCT	1 cut(s)	562
SspI	AATATT	1 cut(s)	2130
SspMI	CTAG	8 cut(s)	212, 881, 983, 1086, 1626, 3138, 3158, 3179
SstI	GAGCTC	1 cut(s)	672
StuI	AGGCCT	1 cut(s)	562
StyD4I	CCNGG	5 cut(s)	291, 998, 1683, 2321, 3018
StyI	CCWWGG	8 cut(s)	572, 737, 1085, 1257, 1333, 1360, 2921, 3352
TaiI	ACGT	1 cut(s)	1762
TaqI	TCGA	2 cut(s)	2135, 2258
TatI	WGTACW	2 cut(s)	1027, 2804
TauI	GCSGC	6 cut(s)	61, 679, 1307, 2231, 2830, 3482
TscAI	CASTG	8 cut(s)	358, 380, 643, 1588, 2314, 2692, 2757, 3259
TseFI	GTSAC	4 cut(s)	497, 782, 2114, 3401
Tsp45I	GTSAC	4 cut(s)	497, 782, 2114, 3401
TspGWI	ACGGA	5 cut(s)	97, 306, 1602, 2698, 3348
TspRI	CASTG	8 cut(s)	358, 380, 643, 1588, 2314, 2692, 2757, 3259
Vha464I	CTTAAG	1 cut(s)	1514
VneI	GTGCAC	2 cut(s)	2688, 3255
VpaK11BI	GGWCC	2 cut(s)	2586, 3356
XagI	CCTNNNNNAGG	1 cut(s)	1514
XapI	RAATTY	5 cut(s)	405, 1383, 2313, 3006, 3464
XbaI	TCTAGA	2 cut(s)	982, 1625
XceI	RCATGY	7 cut(s)	251, 390, 1560, 1980, 2272, 2276, 3263
XcmI	CCANNNNNNNNNTGG	2 cut(s)	436, 1488
XmaJI	CCTAGG	1 cut(s)	1085
XmiI	GTMKAC	2 cut(s)	526, 2100
XmnI	GAANNNNTTC	4 cut(s)	138, 1044, 1546, 1593
XspI	CTAG	8 cut(s)	212, 881, 983, 1086, 1626, 3138, 3158, 3179
ZrmI	AGTACT	1 cut(s)	2806
Zsp2I	ATGCAT	5 cut(s)	278, 1069, 1101, 2274, 2845

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.