MD05G1060000.v1.1

Protein BREAST CANCER SUSCEPTIBILITY 1 homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0008716
Physical Location & Seq
Forward (+)
10464036 .. 10466677
2642 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1060000.v1.1.491

Sequence Viewer

Length: 1173 bp
ATGTGCTTTCTGTCTTGCATCAGAAGAATCAGAGGTCACTACTATAATGGCAAGCCTGTCGCTGCGGATCACAATGGAGGACTCAAGGTTATACATGCACACAGGACCTGCGCTGAATGGGCTCCCAATGTCTACTTTGAAGAAGATACTGCTATTAATCTTGAAGCTGAATTAACTAGGAGTCGTAAAATTAAATGTTCTTGCTGCGCAATTAAAGGAGCAGCTCTTGGGTGCTACGAGAAGAGTTGTCGTAGGAGCTTTCATGTCACCTGTGCAAAGTTGATGCCTCAATGTCGATGGGATACTGACAATTTTGTTATGTTATGCCCCGTTCATGCCTCCTCTAAGTTGCCAAATGAAAGTCCTGAATCTCAAGCAACGAGGAAGAAGAGCAATCCTAAAAAACAACCGAATGTTGAATGTCCTAAAGTTGCTGCTAAACAAGACAGCAATACCCCTCCAGATCAGAAGTTTTGTGGACCGTCCAAGAAATTGGTTCTTTGTTGTTCATCCCTCACAAATGCAGAGAGGGAGTCTGTCGCTGGATTTGAAAGATTATCTGGACTTATGGTTTTGAAGAATTGGGATTCTAATGTTACACATGTTATTGCATCGACAGATGAAAATGGAGCATGCAAAAGGACCCTCAAAGTTTTGATGGGTATCTTGGAGGGGAAGTGGATCCTGAATGTGGAGTGGATTAATGCTTGCACAGAGGCCATGAAACTAGTTGATGAGGAGCCTTATGAAATCAAGGTCGACATTTATGGAATCAGGGATGGTCCTCGACTTGGTAGACTAAGGCTGCAGAACAAGCAACCAAAGCTTTTTGATGGATTCAAGTTTTACTTCATGGGAGACTTTCTACCTTCGTACAAGGGGTATCTACAAGACCTTGTAATAGCTGCCGGAGGAACGATTCTGCAAAGAAAGCCTGTTCCAGAGGGCCAAAAAGCCTTGTCAGCCGGTTCCCCCAAATGTCAAACCTACATAATTTACAGCCTCGAGCTGCCTGATCAATGTCATCGTAGCAAGAAGAGTACGATTTTCAACACTAGGCAGGCTGATGCAAAGGCTTTGGCCAGTTCCGATGGAGCCATGGCAGTGAGCAATTCATGGGTTTTGAACTCCATTGCCGCCTGCAAGTTACAAAATCTTTCTGATTTCGAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000151 GO:0000152 GO:0000724 GO:0000725 GO:0003674 GO:0003824 GO:0004842 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005886 GO:0006139 GO:0006259 GO:0006281 GO:0006302 GO:0006310 GO:0006355 GO:0006357 GO:0006464 GO:0006725 GO:0006807 GO:0006950 GO:0006974 GO:0008150 GO:0008152 GO:0009314 GO:0009628 GO:0009889 GO:0009890 GO:0009891 GO:0009892 GO:0009893 GO:0009987 GO:0010212 GO:0010332 GO:0010468 GO:0010556 GO:0010557 GO:0010564 GO:0010565 GO:0010604 GO:0010605 GO:0010628 GO:0010638 GO:0010639 GO:0016020 GO:0016567 GO:0016740 GO:0019216 GO:0019217 GO:0019219 GO:0019222 GO:0019538 GO:0019787 GO:0031056 GO:0031057 GO:0031058 GO:0031323 GO:0031324 GO:0031325 GO:0031326 GO:0031327 GO:0031328 GO:0031399 GO:0031400 GO:0031401 GO:0031436 GO:0032268 GO:0032269 GO:0032270 GO:0032446 GO:0032991 GO:0033043 GO:0033044 GO:0033554 GO:0034641 GO:0035065 GO:0035066 GO:0035067 GO:0036211 GO:0042127 GO:0042304 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044464 GO:0045717 GO:0045787 GO:0045833 GO:0045893 GO:0045922 GO:0045935 GO:0045944 GO:0046483 GO:0046890 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0050789 GO:0050794 GO:0050896 GO:0051055 GO:0051128 GO:0051129 GO:0051130 GO:0051171 GO:0051172 GO:0051173 GO:0051246 GO:0051247 GO:0051248 GO:0051252 GO:0051254 GO:0051716 GO:0051726 GO:0060255 GO:0062012 GO:0062014 GO:0065007 GO:0070531 GO:0070647 GO:0071156 GO:0071158 GO:0071214 GO:0071478 GO:0071479 GO:0071480 GO:0071704 GO:0071944 GO:0080090 GO:0090068 GO:0090304 GO:0104004 GO:0140096 GO:1901360 GO:1901564 GO:1901983 GO:1901984 GO:1901985 GO:1902275 GO:1902494 GO:1902680 GO:1903506 GO:1903508 GO:1905268 GO:1905269 GO:1990234 GO:2000112 GO:2000756 GO:2000757 GO:2000758 GO:2001141 GO:2001251 GO:2001252
KEGG Pathways
Metabolic & Signaling
ko03440 map03440
Pfam Domains
Protein Families
BRCT BRCT_2 DYW_deaminase PPR zf-C3HC4_2 zf-HC5HC2H zf-RING_2

Protein Analysis

391

Amino Acids

43.43

Weight (kDa)

8.94

Isoelectric Point (pI)

45.6

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-HC5HC2H_2 PF13832 32 - 94 1.2e-07 PHD-zinc-finger like domain
zf-HC5HC2H PF13771 34 - 112 4.6e-11 PHD-like zinc-binding domain
BRCT PF00533 164 - 237 4e-09 BRCA1 C Terminus (BRCT) domain
BRCT_2 PF16589 275 - 390 3.2e-08 BRCT domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 208
Acc36I ACCTGC 1 cut(s) 116
AccI GTMKAC 3 cut(s) 132, 759, 796
AciI CCGC 2 cut(s) 65, 1137
AclWI GGATC 3 cut(s) 75, 676, 689
AcoI YGGCCR 1 cut(s) 1080
AfaI GTAC 2 cut(s) 875, 1042
AfiI CCNNNNNNNGG 2 cut(s) 691, 791
AflIII ACRYGT 1 cut(s) 601
AgsI TTSAA 8 cut(s) 140, 164, 419, 551, 577, 841, 1051, 1126
AhlI ACTAGT 1 cut(s) 727
AluBI AGCT 6 cut(s) 167, 224, 258, 826, 905, 1009
AluI AGCT 6 cut(s) 167, 224, 258, 826, 905, 1009
Alw26I GTCTC 1 cut(s) 852
AlwI GGATC 3 cut(s) 75, 676, 689
AlwNI CAGNNNCTG 1 cut(s) 108
Ama87I CYCGRG 1 cut(s) 1004
AoxI GGCC 3 cut(s) 717, 946, 1080
ApeKI GCWGC 7 cut(s) 62, 204, 221, 434, 805, 905, 1009
AseI ATTAAT 2 cut(s) 156, 702
AspLEI GCGC 2 cut(s) 113, 209
AspS9I GGNCC 5 cut(s) 105, 479, 642, 782, 946
AsuHPI GGTGA 1 cut(s) 259
AsuII TTCGAA 1 cut(s) 1167
AvaI CYCGRG 1 cut(s) 1004
AvaII GGWCC 4 cut(s) 105, 479, 642, 782
BalI TGGCCA 1 cut(s) 1082
BamHI GGATCC 1 cut(s) 681
BanII GRGCYC 1 cut(s) 124
BbvI GCAGC 7 cut(s) 49, 191, 233, 421, 792, 892, 996
BccI CCATC 5 cut(s) 291, 652, 773, 827, 1085
BcgI CGANNNNNNTGC 2 cut(s) 40, 74
BciVI GTATCC 1 cut(s) 295
BclI TGATCA 1 cut(s) 1015
BcoDI GTCTC 1 cut(s) 852
BcuI ACTAGT 1 cut(s) 727
BfaI CTAG 3 cut(s) 177, 728, 1056
BfmI CTRYAG 1 cut(s) 806
BfuAI ACCTGC 1 cut(s) 116
BfuI GTATCC 1 cut(s) 295
BisI GCNGC 8 cut(s) 63, 205, 222, 435, 806, 906, 1010, 1137
BlsI GCNGC 8 cut(s) 64, 206, 223, 436, 807, 907, 1011, 1138
Bme18I GGWCC 4 cut(s) 105, 479, 642, 782
BmeT110I CYCGRG 1 cut(s) 1004
BmgT120I GGNCC 5 cut(s) 105, 479, 642, 782, 946
BmiI GGNNCC 6 cut(s) 123, 644, 683, 741, 970, 1096
BmsI GCATC 4 cut(s) 27, 273, 620, 1057
BpmI CTGGAG 1 cut(s) 444
Bpu14I TTCGAA 1 cut(s) 1167
BpuEI CTTGAG 2 cut(s) 68, 357
BsaBI GATNNNNATC 1 cut(s) 662
BsaJI CCNNGG 1 cut(s) 1098
BsaXI ACNNNNNCTCC 2 cut(s) 662, 692
Bsc4I CCNNNNNNNGG 2 cut(s) 691, 791
Bse118I RCCGGY 1 cut(s) 965
Bse1I ACTGG 1 cut(s) 1083
Bse3DI GCAATG 1 cut(s) 1131
Bse8I GATNNNNATC 1 cut(s) 662
BseDI CCNNGG 1 cut(s) 1098
BseGI GGATG 2 cut(s) 509, 784
BseJI GATNNNNATC 1 cut(s) 662
BseLI CCNNNNNNNGG 2 cut(s) 691, 791
BseMI GCAATG 1 cut(s) 1131
BseNI ACTGG 1 cut(s) 1083
BseRI GAGGAG 2 cut(s) 331, 752
BseXI GCAGC 7 cut(s) 49, 191, 233, 421, 792, 892, 996
BshFI GGCC 3 cut(s) 719, 948, 1082
BsiHKCI CYCGRG 1 cut(s) 1004
BsiSI CCGG 2 cut(s) 909, 966
BslI CCNNNNNNNGG 2 cut(s) 691, 791
BsmAI GTCTC 1 cut(s) 852
BsnI GGCC 3 cut(s) 719, 948, 1082
BsoBI CYCGRG 1 cut(s) 1004
Bsp119I TTCGAA 1 cut(s) 1167
Bsp1286I GDGCHC 1 cut(s) 124
Bsp143I GATC 4 cut(s) 67, 463, 681, 1015
Bsp19I CCATGG 1 cut(s) 1098
BspACI CCGC 2 cut(s) 65, 1137
BspANI GGCC 3 cut(s) 719, 948, 1082
BspLI GGNNCC 6 cut(s) 123, 644, 683, 741, 970, 1096
BspMAI CTGCAG 1 cut(s) 810
BspMI ACCTGC 1 cut(s) 116
BspPI GGATC 3 cut(s) 75, 676, 689
BspQI GCTCTTC 1 cut(s) 383
BspT104I TTCGAA 1 cut(s) 1167
BsrDI GCAATG 1 cut(s) 1131
BsrFI RCCGGY 1 cut(s) 965
BsrI ACTGG 1 cut(s) 1083
BssAI RCCGGY 1 cut(s) 965
BssECI CCNNGG 1 cut(s) 1098
BssMI GATC 4 cut(s) 67, 463, 681, 1015
BssT1I CCWWGG 1 cut(s) 1098
Bst4CI ACNGT 1 cut(s) 483
Bst6I CTCTTC 3 cut(s) 236, 383, 1031
BstBI TTCGAA 1 cut(s) 1167
BstC8I GCNNGC 5 cut(s) 53, 634, 709, 1062, 1141
BstDEI CTNAG 2 cut(s) 345, 800
BstDSI CCRYGG 1 cut(s) 1098
BstF5I GGATG 2 cut(s) 509, 784
BstHHI GCGC 2 cut(s) 113, 209
BstKTI GATC 4 cut(s) 70, 466, 684, 1018
BstMAI GTCTC 1 cut(s) 852
BstMBI GATC 4 cut(s) 67, 463, 681, 1015
BstMWI GCNNNNNNNGC 5 cut(s) 119, 814, 823, 931, 962
BstNSI RCATGY 3 cut(s) 98, 605, 636
BstSFI CTRYAG 1 cut(s) 806
BstV1I GCAGC 7 cut(s) 49, 191, 233, 421, 792, 892, 996
BstX2I RGATCY 1 cut(s) 681
BstXI CCANNNNNNTGG 1 cut(s) 493
BstYI RGATCY 1 cut(s) 681
BsuI GTATCC 1 cut(s) 295
BsuRI GGCC 3 cut(s) 719, 948, 1082
BtgI CCRYGG 1 cut(s) 1098
BtsCI GGATG 2 cut(s) 509, 784
BtsI GCAGTG 1 cut(s) 1110
BtsIMutI CAGTG 1 cut(s) 1110
BveI ACCTGC 1 cut(s) 116
Cac8I GCNNGC 5 cut(s) 53, 634, 709, 1062, 1141
CaiI CAGNNNCTG 1 cut(s) 108
CfoI GCGC 2 cut(s) 113, 209
Cfr10I RCCGGY 1 cut(s) 965
Cfr13I GGNCC 5 cut(s) 105, 479, 642, 782, 946
Csp6I GTAC 2 cut(s) 874, 1041
CviAII CATG 9 cut(s) 95, 263, 335, 602, 633, 721, 853, 1099, 1116
CviQI GTAC 2 cut(s) 874, 1041
DdeI CTNAG 2 cut(s) 345, 800
DpnI GATC 4 cut(s) 69, 465, 683, 1017
DpnII GATC 4 cut(s) 67, 463, 681, 1015
EaeI YGGCCR 1 cut(s) 1080
Eam1104I CTCTTC 3 cut(s) 236, 383, 1031
EarI CTCTTC 3 cut(s) 236, 383, 1031
Eco130I CCWWGG 1 cut(s) 1098
Eco24I GRGCYC 1 cut(s) 124
Eco47I GGWCC 4 cut(s) 105, 479, 642, 782
Eco88I CYCGRG 1 cut(s) 1004
EcoO109I RGGNCCY 2 cut(s) 105, 642
EcoT14I CCWWGG 1 cut(s) 1098
EcoT38I GRGCYC 1 cut(s) 124
ErhI CCWWGG 1 cut(s) 1098
FaeI CATG 9 cut(s) 98, 266, 338, 605, 636, 724, 856, 1102, 1119
FalI AAGNNNNNCTT 2 cut(s) 833, 865
FatI CATG 9 cut(s) 94, 262, 334, 601, 632, 720, 852, 1098, 1115
FbaI TGATCA 1 cut(s) 1015
FblI GTMKAC 3 cut(s) 132, 759, 796
Fnu4HI GCNGC 8 cut(s) 63, 205, 222, 435, 806, 906, 1010, 1137
FokI GGATG 2 cut(s) 496, 791
FriOI GRGCYC 1 cut(s) 124
Fsp4HI GCNGC 8 cut(s) 63, 205, 222, 435, 806, 906, 1010, 1137
FspBI CTAG 3 cut(s) 177, 728, 1056
FspI TGCGCA 1 cut(s) 208
GlaI GCGC 2 cut(s) 112, 208
GluI GCNGC 8 cut(s) 63, 205, 222, 435, 806, 906, 1010, 1137
GsuI CTGGAG 1 cut(s) 444
HaeIII GGCC 3 cut(s) 719, 948, 1082
HapII CCGG 2 cut(s) 909, 966
HhaI GCGC 2 cut(s) 113, 209
Hin1II CATG 9 cut(s) 98, 266, 338, 605, 636, 724, 856, 1102, 1119
Hin6I GCGC 2 cut(s) 111, 207
HinP1I GCGC 2 cut(s) 111, 207
HincII GTYRAC 1 cut(s) 760
HindII GTYRAC 1 cut(s) 760
HindIII AAGCTT 1 cut(s) 824
HinfI GANTC 9 cut(s) 27, 81, 181, 368, 533, 587, 771, 837, 919
HpaII CCGG 2 cut(s) 909, 966
HphI GGTGA 1 cut(s) 259
Hpy166II GTNNAC 4 cut(s) 133, 479, 760, 797
Hpy188I TCNGA 5 cut(s) 23, 32, 468, 1090, 1162
Hpy188III TCNNGA 6 cut(s) 161, 365, 461, 561, 685, 941
Hpy8I GTNNAC 4 cut(s) 133, 479, 760, 797
HpyAV CCTTC 1 cut(s) 879
HpyCH4III ACNGT 1 cut(s) 483
HpyF10VI GCNNNNNNNGC 5 cut(s) 119, 814, 823, 931, 962
HpyF3I CTNAG 2 cut(s) 345, 800
Hsp92II CATG 9 cut(s) 98, 266, 338, 605, 636, 724, 856, 1102, 1119
HspAI GCGC 2 cut(s) 111, 207
Ksp22I TGATCA 1 cut(s) 1015
Kzo9I GATC 4 cut(s) 67, 463, 681, 1015
LguI GCTCTTC 1 cut(s) 383
LmnI GCTCC 6 cut(s) 127, 218, 255, 629, 739, 1094
Lsp1109I GCAGC 7 cut(s) 49, 191, 233, 421, 792, 892, 996
LweI GCATC 4 cut(s) 27, 273, 620, 1057
MaeI CTAG 3 cut(s) 177, 728, 1056
MaeIII GTNAC 4 cut(s) 35, 265, 595, 1146
MalI GATC 4 cut(s) 69, 465, 683, 1017
MboI GATC 4 cut(s) 67, 463, 681, 1015
MboII GAAGA 8 cut(s) 36, 152, 155, 253, 397, 400, 589, 1048
MflI RGATCY 1 cut(s) 681
MhlI GDGCHC 1 cut(s) 124
MlsI TGGCCA 1 cut(s) 1082
MluCI AATT 8 cut(s) 170, 189, 210, 310, 491, 580, 993, 1111
MluNI TGGCCA 1 cut(s) 1082
MlyI GAGTC 3 cut(s) 75, 190, 542
Mox20I TGGCCA 1 cut(s) 1082
MscI TGGCCA 1 cut(s) 1082
MseI TTAA 5 cut(s) 156, 173, 192, 213, 702
MslI CAYNNNNRTG 1 cut(s) 1103
Msp20I TGGCCA 1 cut(s) 1082
MspI CCGG 2 cut(s) 909, 966
MwoI GCNNNNNNNGC 5 cut(s) 119, 814, 823, 931, 962
NcoI CCATGG 1 cut(s) 1098
NdeII GATC 4 cut(s) 67, 463, 681, 1015
NlaIII CATG 9 cut(s) 98, 266, 338, 605, 636, 724, 856, 1102, 1119
NlaIV GGNNCC 6 cut(s) 123, 644, 683, 741, 970, 1096
NmuCI GTSAC 2 cut(s) 35, 265
NsbI TGCGCA 1 cut(s) 208
NspI RCATGY 3 cut(s) 98, 605, 636
NspV TTCGAA 1 cut(s) 1167
PaeI GCATGC 1 cut(s) 636
PaeR7I CTCGAG 1 cut(s) 1004
PciI ACATGT 1 cut(s) 601
PciSI GCTCTTC 1 cut(s) 383
PfeI GAWTC 6 cut(s) 27, 368, 587, 771, 837, 919
PkrI GCNGC 8 cut(s) 64, 206, 223, 436, 807, 907, 1011, 1138
PleI GAGTC 3 cut(s) 75, 189, 541
PpsI GAGTC 3 cut(s) 75, 189, 541
PpuMI RGGWCCY 2 cut(s) 105, 642
PscI ACATGT 1 cut(s) 601
PshBI ATTAAT 2 cut(s) 156, 702
Psp5II RGGWCCY 2 cut(s) 105, 642
PspN4I GGNNCC 6 cut(s) 123, 644, 683, 741, 970, 1096
PspPI GGNCC 5 cut(s) 105, 479, 642, 782, 946
PspPPI RGGWCCY 2 cut(s) 105, 642
PspXI VCTCGAGB 1 cut(s) 1004
PstI CTGCAG 1 cut(s) 810
PstNI CAGNNNCTG 1 cut(s) 108
PsuI RGATCY 1 cut(s) 681
RsaI GTAC 2 cut(s) 875, 1042
RsaNI GTAC 2 cut(s) 874, 1041
RseI CAYNNNNRTG 1 cut(s) 1103
SalI GTCGAC 1 cut(s) 758
SapI GCTCTTC 1 cut(s) 383
SaqAI TTAA 5 cut(s) 156, 173, 192, 213, 702
SatI GCNGC 8 cut(s) 63, 205, 222, 435, 806, 906, 1010, 1137
Sau3AI GATC 4 cut(s) 67, 463, 681, 1015
Sau96I GGNCC 5 cut(s) 105, 479, 642, 782, 946
SchI GAGTC 3 cut(s) 75, 190, 542
SduI GDGCHC 1 cut(s) 124
SfaNI GCATC 4 cut(s) 27, 273, 620, 1057
SfcI CTRYAG 1 cut(s) 806
Sfr274I CTCGAG 1 cut(s) 1004
SfuI TTCGAA 1 cut(s) 1167
SinI GGWCC 4 cut(s) 105, 479, 642, 782
SlaI CTCGAG 1 cut(s) 1004
SmiMI CAYNNNNRTG 1 cut(s) 1103
SmlI CTYRAG 3 cut(s) 83, 372, 1004
SmoI CTYRAG 3 cut(s) 83, 372, 1004
SpeI ACTAGT 1 cut(s) 727
SphI GCATGC 1 cut(s) 636
Sse9I AATT 8 cut(s) 170, 189, 210, 310, 491, 580, 993, 1111
SsiI CCGC 2 cut(s) 65, 1137
SspMI CTAG 3 cut(s) 177, 728, 1056
StyI CCWWGG 1 cut(s) 1098
TaaI ACNGT 1 cut(s) 483
TaqI TCGA 6 cut(s) 295, 614, 759, 787, 1005, 1167
TasI AATT 8 cut(s) 170, 189, 210, 310, 491, 580, 993, 1111
TauI GCSGC 1 cut(s) 1139
TfiI GAWTC 6 cut(s) 27, 368, 587, 771, 837, 919
Tru1I TTAA 5 cut(s) 156, 173, 192, 213, 702
Tru9I TTAA 5 cut(s) 156, 173, 192, 213, 702
TscAI CASTG 1 cut(s) 1110
TseFI GTSAC 2 cut(s) 35, 265
TseI GCWGC 7 cut(s) 62, 204, 221, 434, 805, 905, 1009
Tsp45I GTSAC 2 cut(s) 35, 265
TspDTI ATGAA 9 cut(s) 251, 323, 372, 498, 636, 737, 762, 841, 1104
TspRI CASTG 1 cut(s) 1110
VpaK11BI GGWCC 4 cut(s) 105, 479, 642, 782
VspI ATTAAT 2 cut(s) 156, 702
XceI RCATGY 3 cut(s) 98, 605, 636
XhoI CTCGAG 1 cut(s) 1004
XmiI GTMKAC 3 cut(s) 132, 759, 796
XspI CTAG 3 cut(s) 177, 728, 1056
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.