MD10G1256100.v1.1

Protein BREAST CANCER SUSCEPTIBILITY 1 homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0008716
Physical Location & Seq
Reverse (-)
35053220 .. 35058750
5531 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1256100.v1.1.491

Sequence Viewer

Length: 3057 bp
ATGGAGACTCCTCCGGCCCACCTCGAGAAGATCGGCAGAGAACTCAAGTGCCCCATTTGTTTGAGTCTGCTGAGTTCTGCGGTTTCACTCAATTGCAATCATCTCTTCTGCAACTGTTGCATTGTGAAGTCGATGAAATCGGGTTCCAATTGTCCTGTTTGTAAAGTCCCATATCGGCGTAGAGAGGTTCGCCCGGCTCCTCACATGGATAATTTGGTTAGTATTTACAAGAGCATGGAAGATGATTTGGGAATTAATATATTTGTCACTCAGAATGCACCCCCAACTAAATCATCAGACGGAAAACATCATGCTGAAGATGATTTTAGCCACGGCTTTCAGGATAGAGCGCAGAAACAGAAAACCTTAAGAGGAAAAAGATCCAGGAAAACTAAGGCCAACCTGGAAAGTTCTGGTTCTATTTCAATTAAACCTTCATTTCCAAACAAGAAAAGGGTCCAGGTTCCACAGTCTCCTCTTTCAGAAACCCCAACCCGCCATGAAAAGTTAGAAGGTGATTTAATGGCTGATCGAGTGAAGCAGAGTTCAAAAACACTAAAGGAGAAGCCTGTTCTAAATGAGAAGGGAGAACTGGTTCTGTCACCTTTCTTTTGGTTGAATGACAAAGATGTAGAAAATTTGAGTCAGCATTCTTCCGGTGATCAACTTAGCGATCTTCCATCTCCAAATGTTCCCACATTCAGTGATATCAAAGACTCTGATGATGAAAATTCTGTTAGGTCCCTACCGGGAGAAGTGCACAGCACATCTTCTAATGCTGCAGATCTATTTGATAGTGAGATGTTTGAATGGACACAAAGAGCTTGCTCTCCTGAACTCTTCCCAAGTCCTGAGATGCAGGTTGCAGACGATGAACACGATAGAGTTCAAGAGAAGGAATCAAAAGAAGTCTCACAAAACAAGAAATCAGATGAACTTTCTGTAAAAAGTGCAAGGTCTAAAAGAAACAAAAAAAGTAATGGAAATATGGATTTTCTGCCAGATCTTCCCACTGCTGGAACTAAAGATGTTAGTCATGAAGTTGTTAGTAATGAATCTGACAAGCGAGTTAGGAAGACAAGGAATCTCATGAAAAGAAAATGTGCTACAAGCCATACAGATCCAGGAACAAATATTAGTGTCACTTCAAAGGGATCTGAGGTATTTTATGAAGAACAAGCATGTGAGAATAACGGTGGTTCTTTAGCTAAGACTTGCAAAAGGAGTAAGAAGGGACATTCATCTGTTGTAACCAAGCCCACATCTGAAAATGTTCATGTCCTCTCTACCAGAGCTGATAGTCGAATTAATGGTGATGAAAATAGGGTAACTGAATCACCTACTTCAATAGGAAAAAATGCTGGCAGTGATGAACTGTCCAGTAAGAAGGCTGGAAATATCTATAAGGAGGTCAATGACAAGAACAAGATTGACCGCCCAGTAAGGTCAAAAAAACAAAAGATGGTCTCCATGAAAAAGACAATGCTTGAGGATGTATCAGAAATACAGAAGCAAGCAAATAAGGATACTCCTACTGAGTTGTCTCCTACAAATCTTCCCACAGATGATAACCAGAAAGCCTCTGAATCCAGGAATAAATCCAGAAAACTTGCTAGAAAAGCCAAGTCAGGTGATCGAGAACTGAAAAGTAAGAAAAAGCTGAAAGCTTCTTCTGATGGCAACTCAAAGGATGATACTTCTGTTGAGATTGGAGAAGGTCATGGGAATGTTAACGAAAATGGAAATGAAACCGCTGAGAAGAGCAAGGACAATCATAATGTTATAACTGATCAATCACCAGTGCAGAAGCTTCTTTCATTTACAAACAATGTGGTTTTGAAGAGATGTGAAGCTGTTCCTAGTAATATCCAGTGTGCTTTCTGTCTTTCATCAGAAGAATCAGAGGCTTCTGGAGAGATAGTCCACTATTGTAATGGCAAGCCTGTTGCTGCGGATCACGATGGAAGACTCAAGGTTATACATGTACACAGGACCTGTGCTGAATGGGCTCCTAATGTCTATTTTGAAGATGATATTGCTATTAATCTTGAAGCTGAATTAACTAGGAGTCGTAAAATTAAATGTTCTTGCTGCGCAATCAAAGGAGCTGCTCTTGGGTGCTGTGAGAAGAGTTGTCGTAGGAGCTTTCATGTTACCTGTGCAAAGCTGATGCCTCAATGTCGCTGGGATACTGATAACTTTGTTATGTTATGCCCCATTCATGCCTCCTCCAATTTGCCAAATGAAAGCCCTGGATCTCAAGCAAGGAGGAAGAAGAATCCTAGAAAACAACCTAATGTTGAACGTCCTAAAGTTGCTGCTAAACAAGACAGCAATATCCCCCCAGATCAGAAGTTTTGTGGATCGTCCAAGAAATTGATTCTTTGTTGTTCATCTCTCACAAATGCAGAGAGGGAATCTGTCTCTGGATTTGAAAGATTATCTGGACTTACGGTTTTGAAGAATTGGGATTCTAGTGTTACACATATTATTGCATCGACAGATGAAAATGGAGCATGCAGAAGAACCCTCAAAGTTTTGATGGGTATCTTGGAGGGGAAGTGGATTCTGAGTGTGGAGTGGATTAATGCTTGCACAGAAGCAATGAAACTAGTTGATGAGGAGCCTTATGAAATCAACGTTGACATTTATGGAATCAGGGATGGTCCTCGACTTGGTAGACTAAGGCTGCAGAACAAGCAACCAAAACTTTTTGAGGGGTTCAAGTTTTACTTCATGGGAGACTTCATACCTTCATACAAGGGGTATTTACAAGACCTTGTAATAGCTGCTGGAGGAACGATTTTGCAAAGAAAACCTGTTCCAGAGGGCCCGAAAGCCTTGTCAGCCGGTTCCCCCAAATGTCAAACCTTCATAATTTACAGCCTCGAGCTACCTGACCAATGTCATTGCAGCAAGAAGAGTACAATTTTCAACACTAGGCAGGCTGATGCGAAGGCTTTGGCCACCTCCACTGGAGCCAAGGTAGCAAGCAACTCATGGGTATTGAACTCCATTGCTGCCTGCAAGTTACAAAATCTCTCTGATTCCAAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000151 GO:0000152 GO:0000724 GO:0000725 GO:0003674 GO:0003824 GO:0004842 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005886 GO:0006139 GO:0006259 GO:0006281 GO:0006302 GO:0006310 GO:0006355 GO:0006357 GO:0006464 GO:0006725 GO:0006807 GO:0006950 GO:0006974 GO:0008150 GO:0008152 GO:0009314 GO:0009628 GO:0009889 GO:0009890 GO:0009891 GO:0009892 GO:0009893 GO:0009987 GO:0010212 GO:0010332 GO:0010468 GO:0010556 GO:0010557 GO:0010564 GO:0010565 GO:0010604 GO:0010605 GO:0010628 GO:0010638 GO:0010639 GO:0016020 GO:0016567 GO:0016740 GO:0019216 GO:0019217 GO:0019219 GO:0019222 GO:0019538 GO:0019787 GO:0031056 GO:0031057 GO:0031058 GO:0031323 GO:0031324 GO:0031325 GO:0031326 GO:0031327 GO:0031328 GO:0031399 GO:0031400 GO:0031401 GO:0031436 GO:0032268 GO:0032269 GO:0032270 GO:0032446 GO:0032991 GO:0033043 GO:0033044 GO:0033554 GO:0034641 GO:0035065 GO:0035066 GO:0035067 GO:0036211 GO:0042127 GO:0042304 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044464 GO:0045717 GO:0045787 GO:0045833 GO:0045893 GO:0045922 GO:0045935 GO:0045944 GO:0046483 GO:0046890 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0050789 GO:0050794 GO:0050896 GO:0051055 GO:0051128 GO:0051129 GO:0051130 GO:0051171 GO:0051172 GO:0051173 GO:0051246 GO:0051247 GO:0051248 GO:0051252 GO:0051254 GO:0051716 GO:0051726 GO:0060255 GO:0062012 GO:0062014 GO:0065007 GO:0070531 GO:0070647 GO:0071156 GO:0071158 GO:0071214 GO:0071478 GO:0071479 GO:0071480 GO:0071704 GO:0071944 GO:0080090 GO:0090068 GO:0090304 GO:0104004 GO:0140096 GO:1901360 GO:1901564 GO:1901983 GO:1901984 GO:1901985 GO:1902275 GO:1902494 GO:1902680 GO:1903506 GO:1903508 GO:1905268 GO:1905269 GO:1990234 GO:2000112 GO:2000756 GO:2000757 GO:2000758 GO:2001141 GO:2001251 GO:2001252
KEGG Pathways
Metabolic & Signaling
ko03440 map03440
Pfam Domains
Protein Families
BRCT BRCT_2 DYW_deaminase PPR zf-C3HC4_2 zf-HC5HC2H zf-RING_2

Protein Analysis

1019

Amino Acids

112.73

Weight (kDa)

8.87

Isoelectric Point (pI)

48.41

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-C3HC4_2 PF13923 16 - 54 2.6e-08 Zinc finger, C3HC4 type (RING finger)
zf-RING_2 PF13639 16 - 55 3.6e-06 Ring finger domain
zf-C3HC4 PF00097 17 - 54 1.2e-06 Zinc finger, C3HC4 type (RING finger)
zf-HC5HC2H_2 PF13832 661 - 723 2.8e-06 PHD-zinc-finger like domain
zf-HC5HC2H PF13771 663 - 741 5.1e-10 PHD-like zinc-binding domain
BRCT PF00533 792 - 865 2.7e-09 BRCA1 C Terminus (BRCT) domain
BRCT_2 PF16589 903 - 1016 1.7e-07 BRCT domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1784
Acc16I TGCGCA 1 cut(s) 2095
Acc36I ACCTGC 1 cut(s) 850
AccI GTMKAC 1 cut(s) 2680
AciI CCGC 5 cut(s) 80, 496, 1435, 1752, 1952
AclI AACGTT 1 cut(s) 2640
AclWI GGATC 6 cut(s) 375, 1115, 1162, 1962, 2263, 2371
AcoI YGGCCR 1 cut(s) 2964
AcsI RAATTY 2 cut(s) 637, 730
AcuI CTGAAG 1 cut(s) 336
AfaI GTAC 2 cut(s) 1986, 2926
AfiI CCNNNNNNNGG 2 cut(s) 1016, 2675
AflII CTTAAG 1 cut(s) 367
AflIII ACRYGT 1 cut(s) 1981
AhlI ACTAGT 1 cut(s) 2611
AjnI CCWGG 6 cut(s) 383, 402, 459, 1123, 1589, 2251
AloI GAACNNNNNNTCC 2 cut(s) 579, 611
Alw21I GWGCWC 1 cut(s) 762
Alw26I GTCTC 6 cut(s) 477, 916, 1471, 1548, 2428, 2736
Alw44I GTGCAC 1 cut(s) 758
AlwI GGATC 6 cut(s) 375, 1115, 1162, 1962, 2263, 2371
AlwNI CAGNNNCTG 1 cut(s) 1995
Ama87I CYCGRG 2 cut(s) 23, 2888
AoxI GGCC 4 cut(s) 15, 396, 2830, 2964
ApaI GGGCCC 1 cut(s) 2834
ApaLI GTGCAC 1 cut(s) 758
ApeKI GCWGC 9 cut(s) 779, 1949, 2091, 2108, 2318, 2689, 2789, 2913, 3020
ApoI RAATTY 2 cut(s) 637, 730
ArsI GACNNNNNNTTYG 2 cut(s) 2828, 2860
AseI ATTAAT 4 cut(s) 255, 1308, 2043, 2586
Asp700I GAANNNNTTC 3 cut(s) 594, 1272, 1854
AspLEI GCGC 2 cut(s) 352, 2096
AspS9I GGNCC 7 cut(s) 16, 457, 741, 1992, 2666, 2830, 2831
AsuC2I CCSGG 2 cut(s) 194, 750
AsuHPI GGTGA 7 cut(s) 527, 594, 671, 1325, 1329, 1643, 1788
AvaI CYCGRG 2 cut(s) 23, 2888
AvaII GGWCC 4 cut(s) 457, 741, 1992, 2666
BaeGI GKGCMC 3 cut(s) 53, 762, 2834
BaeI ACNNNNGTAYC 2 cut(s) 1686, 1719
BalI TGGCCA 1 cut(s) 2966
BanII GRGCYC 2 cut(s) 2011, 2834
BbsI GAAGAC 2 cut(s) 1082, 1972
Bbv12I GWGCWC 1 cut(s) 762
BbvI GCAGC 9 cut(s) 766, 1936, 2078, 2095, 2305, 2676, 2776, 2925, 3007
BccI CCATC 6 cut(s) 688, 1456, 1670, 1955, 2536, 2657
BceAI ACGGC 1 cut(s) 349
BciT130I CCWGG 6 cut(s) 385, 404, 461, 1125, 1591, 2253
BciVI GTATCC 2 cut(s) 1519, 2182
BclI TGATCA 2 cut(s) 661, 1789
BcnI CCSGG 2 cut(s) 194, 750
BcoDI GTCTC 6 cut(s) 477, 916, 1471, 1548, 2428, 2736
BcuI ACTAGT 1 cut(s) 2611
BfaI CTAG 7 cut(s) 1614, 1860, 2064, 2283, 2475, 2612, 2940
BfmI CTRYAG 2 cut(s) 780, 2690
BfrI CTTAAG 1 cut(s) 367
BfuAI ACCTGC 1 cut(s) 850
BfuI GTATCC 2 cut(s) 1519, 2182
BglII AGATCT 2 cut(s) 784, 1003
BisI GCNGC 9 cut(s) 780, 1950, 2092, 2109, 2319, 2690, 2790, 2914, 3021
BlsI GCNGC 9 cut(s) 781, 1951, 2093, 2110, 2320, 2691, 2791, 2915, 3022
Bme1390I CCNGG 8 cut(s) 194, 385, 404, 461, 750, 1125, 1591, 2253
Bme18I GGWCC 4 cut(s) 457, 741, 1992, 2666
BmeT110I CYCGRG 2 cut(s) 23, 2888
BmgT120I GGNCC 7 cut(s) 16, 457, 741, 1992, 2666, 2830, 2831
BmrFI CCNGG 8 cut(s) 194, 385, 404, 461, 750, 1125, 1591, 2253
BmrI ACTGGG 1 cut(s) 1433
BmsI GCATC 4 cut(s) 846, 2160, 2504, 2941
BmuI ACTGGG 1 cut(s) 1433
BoxI GACNNNNGTC 1 cut(s) 2904
BpiI GAAGAC 2 cut(s) 1082, 1972
BpmI CTGGAG 3 cut(s) 1932, 2814, 2997
BpuEI CTTGAG 4 cut(s) 29, 1508, 1955, 2244
BpuMI CCSGG 2 cut(s) 194, 750
BsaBI GATNNNNATC 1 cut(s) 2546
BsaI GGTCTC 1 cut(s) 1471
BsaJI CCNNGG 3 cut(s) 331, 2251, 2982
BsaWI WCCGGW 1 cut(s) 656
BsaXI ACNNNNNCTCC 8 cut(s) 579, 609, 1905, 1935, 2546, 2576, 2970, 3000
Bsc4I CCNNNNNNNGG 2 cut(s) 1016, 2675
Bse118I RCCGGY 1 cut(s) 2849
Bse1I ACTGG 6 cut(s) 597, 1380, 1439, 1799, 1870, 2980
Bse3DI GCAATG 3 cut(s) 2610, 2908, 3015
Bse8I GATNNNNATC 1 cut(s) 2546
BseBI CCWGG 6 cut(s) 385, 404, 461, 1125, 1591, 2253
BseDI CCNNGG 3 cut(s) 331, 2251, 2982
BseGI GGATG 3 cut(s) 1498, 1696, 2668
BseJI GATNNNNATC 1 cut(s) 2546
BseLI CCNNNNNNNGG 2 cut(s) 1016, 2675
BseMI GCAATG 3 cut(s) 2610, 2908, 3015
BseMII CTCAG 7 cut(s) 62, 284, 843, 1149, 1527, 1746, 2561
BseNI ACTGG 6 cut(s) 597, 1380, 1439, 1799, 1870, 2980
BseRI GAGGAG 4 cut(s) 189, 465, 2218, 2636
BseSI GKGCMC 3 cut(s) 53, 762, 2834
BseXI GCAGC 9 cut(s) 766, 1936, 2078, 2095, 2305, 2676, 2776, 2925, 3007
BseYI CCCAGC 1 cut(s) 2184
BsgI GTGCAG 1 cut(s) 1823
BshFI GGCC 4 cut(s) 17, 398, 2832, 2966
BsiHKAI GWGCWC 1 cut(s) 762
BsiHKCI CYCGRG 2 cut(s) 23, 2888
BsiSI CCGG 5 cut(s) 14, 194, 657, 749, 2850
BslFI GGGAC 3 cut(s) 152, 727, 1248
BslI CCNNNNNNNGG 2 cut(s) 1016, 2675
BsmAI GTCTC 6 cut(s) 477, 916, 1471, 1548, 2428, 2736
BsmFI GGGAC 3 cut(s) 152, 727, 1248
BsmI GAATGC 2 cut(s) 280, 649
BsnI GGCC 4 cut(s) 17, 398, 2832, 2966
Bso31I GGTCTC 1 cut(s) 1471
BsoBI CYCGRG 2 cut(s) 23, 2888
Bsp120I GGGCCC 1 cut(s) 2830
Bsp1286I GDGCHC 4 cut(s) 53, 762, 2011, 2834
Bsp1407I TGTACA 1 cut(s) 1984
BspACI CCGC 5 cut(s) 80, 496, 1435, 1752, 1952
BspANI GGCC 4 cut(s) 17, 398, 2832, 2966
BspCNI CTCAG 7 cut(s) 63, 283, 844, 1150, 1528, 1747, 2562
BspHI TCATGA 2 cut(s) 1036, 1089
BspMAI CTGCAG 2 cut(s) 784, 2694
BspMI ACCTGC 1 cut(s) 850
BspPI GGATC 6 cut(s) 375, 1115, 1162, 1962, 2263, 2371
BspQI GCTCTTC 1 cut(s) 1754
BspTI CTTAAG 1 cut(s) 367
BspTNI GGTCTC 1 cut(s) 1471
BsrDI GCAATG 3 cut(s) 2610, 2908, 3015
BsrFI RCCGGY 1 cut(s) 2849
BsrGI TGTACA 1 cut(s) 1984
BsrI ACTGG 6 cut(s) 597, 1380, 1439, 1799, 1870, 2980
BssAI RCCGGY 1 cut(s) 2849
BssECI CCNNGG 3 cut(s) 331, 2251, 2982
BssT1I CCWWGG 1 cut(s) 2982
Bst2UI CCWGG 6 cut(s) 385, 404, 461, 1125, 1591, 2253
Bst4CI ACNGT 5 cut(s) 116, 471, 1196, 1377, 2455
Bst6I CTCTTC 6 cut(s) 110, 845, 1754, 1835, 2123, 2915
BstAFI CTTAAG 1 cut(s) 367
BstAPI GCANNNNNTGC 1 cut(s) 117
BstAUI TGTACA 1 cut(s) 1984
BstC8I GCNNGC 9 cut(s) 826, 1363, 1515, 1940, 2518, 2593, 2946, 2992, 3025
BstDSI CCRYGG 1 cut(s) 331
BstF5I GGATG 3 cut(s) 1498, 1696, 2668
BstHHI GCGC 2 cut(s) 352, 2096
BstMAI GTCTC 6 cut(s) 477, 916, 1471, 1548, 2428, 2736
BstMWI GCNNNNNNNGC 6 cut(s) 117, 1619, 2006, 2698, 2846, 2987
BstNI CCWGG 6 cut(s) 385, 404, 461, 1125, 1591, 2253
BstNSI RCATGY 3 cut(s) 1185, 1985, 2520
BstPAI GACNNNNGTC 1 cut(s) 2904
BstSCI CCNGG 8 cut(s) 192, 383, 402, 459, 748, 1123, 1589, 2251
BstSFI CTRYAG 2 cut(s) 780, 2690
BstSLI GKGCMC 3 cut(s) 53, 762, 2834
BstV1I GCAGC 9 cut(s) 766, 1936, 2078, 2095, 2305, 2676, 2776, 2925, 3007
BstV2I GAAGAC 2 cut(s) 1082, 1972
BstX2I RGATCY 6 cut(s) 380, 784, 1003, 1120, 1154, 2255
BstYI RGATCY 6 cut(s) 380, 784, 1003, 1120, 1154, 2255
BsuI GTATCC 2 cut(s) 1519, 2182
BsuRI GGCC 4 cut(s) 17, 398, 2832, 2966
BtgI CCRYGG 1 cut(s) 331
BtsCI GGATG 3 cut(s) 1498, 1696, 2668
BtsI GCAGTG 2 cut(s) 1011, 1372
BtsIMutI CAGTG 6 cut(s) 709, 1011, 1372, 1806, 1877, 2973
BveI ACCTGC 1 cut(s) 850
Cac8I GCNNGC 9 cut(s) 826, 1363, 1515, 1940, 2518, 2593, 2946, 2992, 3025
CaiI CAGNNNCTG 1 cut(s) 1995
CciI TCATGA 2 cut(s) 1036, 1089
CfoI GCGC 2 cut(s) 352, 2096
Cfr10I RCCGGY 1 cut(s) 2849
Cfr13I GGNCC 7 cut(s) 16, 457, 741, 1992, 2666, 2830, 2831
Csp6I GTAC 2 cut(s) 1985, 2925
CspCI CAANNNNNGTGG 2 cut(s) 1812, 1847
CviQI GTAC 2 cut(s) 1985, 2925
EaeI YGGCCR 1 cut(s) 2964
Eam1104I CTCTTC 6 cut(s) 110, 845, 1754, 1835, 2123, 2915
EarI CTCTTC 6 cut(s) 110, 845, 1754, 1835, 2123, 2915
Eco130I CCWWGG 1 cut(s) 2982
Eco24I GRGCYC 2 cut(s) 2011, 2834
Eco31I GGTCTC 1 cut(s) 1471
Eco32I GATATC 1 cut(s) 709
Eco47I GGWCC 4 cut(s) 457, 741, 1992, 2666
Eco57I CTGAAG 1 cut(s) 336
Eco88I CYCGRG 2 cut(s) 23, 2888
EcoO109I RGGNCCY 3 cut(s) 741, 1992, 2830
EcoRII CCWGG 6 cut(s) 383, 402, 459, 1123, 1589, 2251
EcoRV GATATC 1 cut(s) 709
EcoT14I CCWWGG 1 cut(s) 2982
EcoT38I GRGCYC 2 cut(s) 2011, 2834
ErhI CCWWGG 1 cut(s) 2982
FalI AAGNNNNNCTT 2 cut(s) 2717, 2749
FaqI GGGAC 3 cut(s) 152, 727, 1248
FauI CCCGC 1 cut(s) 503
FbaI TGATCA 2 cut(s) 661, 1789
FblI GTMKAC 1 cut(s) 2680
Fnu4HI GCNGC 9 cut(s) 780, 1950, 2092, 2109, 2319, 2690, 2790, 2914, 3021
FokI GGATG 3 cut(s) 1505, 1703, 2675
FriOI GRGCYC 2 cut(s) 2011, 2834
Fsp4HI GCNGC 9 cut(s) 780, 1950, 2092, 2109, 2319, 2690, 2790, 2914, 3021
FspBI CTAG 7 cut(s) 1614, 1860, 2064, 2283, 2475, 2612, 2940
FspI TGCGCA 1 cut(s) 2095
GlaI GCGC 2 cut(s) 351, 2095
GluI GCNGC 9 cut(s) 780, 1950, 2092, 2109, 2319, 2690, 2790, 2914, 3021
GsaI CCCAGC 1 cut(s) 2188
GsuI CTGGAG 3 cut(s) 1932, 2814, 2997
HaeIII GGCC 4 cut(s) 17, 398, 2832, 2966
HapII CCGG 5 cut(s) 14, 194, 657, 749, 2850
HhaI GCGC 2 cut(s) 352, 2096
Hin6I GCGC 2 cut(s) 350, 2094
HinP1I GCGC 2 cut(s) 350, 2094
HincII GTYRAC 2 cut(s) 1732, 2644
HindII GTYRAC 2 cut(s) 1732, 2644
HindIII AAGCTT 2 cut(s) 1665, 1808
HpaI GTTAAC 1 cut(s) 1732
HpaII CCGG 5 cut(s) 14, 194, 657, 749, 2850
HphI GGTGA 7 cut(s) 527, 594, 671, 1325, 1329, 1643, 1788
Hpy166II GTNNAC 6 cut(s) 760, 1732, 1924, 1987, 2644, 2681
Hpy8I GTNNAC 6 cut(s) 760, 1732, 1924, 1987, 2644, 2681
HpyCH4III ACNGT 5 cut(s) 116, 471, 1196, 1377, 2455
HpyCH4IV ACGT 2 cut(s) 2305, 2640
HpyF10VI GCNNNNNNNGC 6 cut(s) 117, 1619, 2006, 2698, 2846, 2987
HpySE526I ACGT 2 cut(s) 2305, 2640
HspAI GCGC 2 cut(s) 350, 2094
Ksp22I TGATCA 2 cut(s) 661, 1789
KspAI GTTAAC 1 cut(s) 1732
LguI GCTCTTC 1 cut(s) 1754
LmnI GCTCC 7 cut(s) 202, 2014, 2105, 2142, 2513, 2623, 2978
Lsp1109I GCAGC 9 cut(s) 766, 1936, 2078, 2095, 2305, 2676, 2776, 2925, 3007
LweI GCATC 4 cut(s) 846, 2160, 2504, 2941
MaeI CTAG 7 cut(s) 1614, 1860, 2064, 2283, 2475, 2612, 2940
MaeII ACGT 2 cut(s) 2305, 2640
MaeIII GTNAC 8 cut(s) 265, 600, 1141, 1249, 1327, 2152, 2479, 3030
MfeI CAATTG 2 cut(s) 91, 148
MflI RGATCY 6 cut(s) 380, 784, 1003, 1120, 1154, 2255
MhlI GDGCHC 4 cut(s) 53, 762, 2011, 2834
MlsI TGGCCA 1 cut(s) 2966
MluNI TGGCCA 1 cut(s) 2966
MlyI GAGTC 5 cut(s) 73, 652, 710, 1962, 2077
Mox20I TGGCCA 1 cut(s) 2966
MroXI GAANNNNTTC 3 cut(s) 594, 1272, 1854
MscI TGGCCA 1 cut(s) 2966
MslI CAYNNNNRTG 1 cut(s) 1725
Msp20I TGGCCA 1 cut(s) 2966
MspA1I CMGCKG 1 cut(s) 1754
MspCI CTTAAG 1 cut(s) 367
MspI CCGG 5 cut(s) 14, 194, 657, 749, 2850
MspR9I CCNGG 8 cut(s) 194, 385, 404, 461, 750, 1125, 1591, 2253
MunI CAATTG 2 cut(s) 91, 148
Mva1269I GAATGC 2 cut(s) 280, 649
MvaI CCWGG 6 cut(s) 385, 404, 461, 1125, 1591, 2253
MwoI GCNNNNNNNGC 6 cut(s) 117, 1619, 2006, 2698, 2846, 2987
NciI CCSGG 2 cut(s) 194, 750
NmuCI GTSAC 3 cut(s) 265, 600, 1141
NsbI TGCGCA 1 cut(s) 2095
NspI RCATGY 3 cut(s) 1185, 1985, 2520
PaeI GCATGC 1 cut(s) 2520
PaeR7I CTCGAG 2 cut(s) 23, 2888
PagI TCATGA 2 cut(s) 1036, 1089
PciI ACATGT 1 cut(s) 1981
PciSI GCTCTTC 1 cut(s) 1754
PcsI WCGNNNNNNNCGW 1 cut(s) 876
PctI GAATGC 2 cut(s) 280, 649
PdmI GAANNNNTTC 3 cut(s) 594, 1272, 1854
PfoI TCCNGGA 3 cut(s) 383, 1123, 1589
PkrI GCNGC 9 cut(s) 781, 1951, 2093, 2110, 2320, 2691, 2791, 2915, 3022
PleI GAGTC 5 cut(s) 72, 651, 710, 1962, 2076
PpsI GAGTC 5 cut(s) 72, 651, 710, 1962, 2076
PpuMI RGGWCCY 2 cut(s) 741, 1992
PscI ACATGT 1 cut(s) 1981
PshAI GACNNNNGTC 1 cut(s) 2904
PshBI ATTAAT 4 cut(s) 255, 1308, 2043, 2586
PsiI TTATAA 1 cut(s) 1784
Psp1406I AACGTT 1 cut(s) 2640
Psp5II RGGWCCY 2 cut(s) 741, 1992
Psp6I CCWGG 6 cut(s) 383, 402, 459, 1123, 1589, 2251
PspFI CCCAGC 1 cut(s) 2184
PspGI CCWGG 6 cut(s) 383, 402, 459, 1123, 1589, 2251
PspOMI GGGCCC 1 cut(s) 2830
PspPI GGNCC 7 cut(s) 16, 457, 741, 1992, 2666, 2830, 2831
PspPPI RGGWCCY 2 cut(s) 741, 1992
PspXI VCTCGAGB 1 cut(s) 2888
PstI CTGCAG 2 cut(s) 784, 2694
PstNI CAGNNNCTG 1 cut(s) 1995
PsuI RGATCY 6 cut(s) 380, 784, 1003, 1120, 1154, 2255
RsaI GTAC 2 cut(s) 1986, 2926
RsaNI GTAC 2 cut(s) 1985, 2925
RseI CAYNNNNRTG 1 cut(s) 1725
SapI GCTCTTC 1 cut(s) 1754
SatI GCNGC 9 cut(s) 780, 1950, 2092, 2109, 2319, 2690, 2790, 2914, 3021
Sau96I GGNCC 7 cut(s) 16, 457, 741, 1992, 2666, 2830, 2831
SchI GAGTC 5 cut(s) 73, 652, 710, 1962, 2077
ScrFI CCNGG 8 cut(s) 194, 385, 404, 461, 750, 1125, 1591, 2253
SduI GDGCHC 4 cut(s) 53, 762, 2011, 2834
SfaNI GCATC 4 cut(s) 846, 2160, 2504, 2941
SfcI CTRYAG 2 cut(s) 780, 2690
Sfr274I CTCGAG 2 cut(s) 23, 2888
SinI GGWCC 4 cut(s) 457, 741, 1992, 2666
SlaI CTCGAG 2 cut(s) 23, 2888
SmiMI CAYNNNNRTG 1 cut(s) 1725
SmlI CTYRAG 7 cut(s) 23, 44, 367, 1487, 1970, 2259, 2888
SmoI CTYRAG 7 cut(s) 23, 44, 367, 1487, 1970, 2259, 2888
SpeI ACTAGT 1 cut(s) 2611
SphI GCATGC 1 cut(s) 2520
SsiI CCGC 5 cut(s) 80, 496, 1435, 1752, 1952
SspI AATATT 1 cut(s) 1135
SspMI CTAG 7 cut(s) 1614, 1860, 2064, 2283, 2475, 2612, 2940
StyD4I CCNGG 8 cut(s) 192, 383, 402, 459, 748, 1123, 1589, 2251
StyI CCWWGG 1 cut(s) 2982
TaaI ACNGT 5 cut(s) 116, 471, 1196, 1377, 2455
TaiI ACGT 2 cut(s) 2308, 2643
TaqI TCGA 8 cut(s) 24, 131, 532, 1303, 1636, 2498, 2671, 2889
TatI WGTACW 2 cut(s) 1984, 2924
TscAI CASTG 6 cut(s) 709, 1018, 1372, 1806, 1877, 2980
TseFI GTSAC 3 cut(s) 265, 600, 1141
TseI GCWGC 9 cut(s) 779, 1949, 2091, 2108, 2318, 2689, 2789, 2913, 3020
Tsp45I GTSAC 3 cut(s) 265, 600, 1141
TspGWI ACGGA 1 cut(s) 315
TspRI CASTG 6 cut(s) 709, 1018, 1372, 1806, 1877, 2980
Vha464I CTTAAG 1 cut(s) 367
VneI GTGCAC 1 cut(s) 758
VpaK11BI GGWCC 4 cut(s) 457, 741, 1992, 2666
VspI ATTAAT 4 cut(s) 255, 1308, 2043, 2586
XapI RAATTY 2 cut(s) 637, 730
XceI RCATGY 3 cut(s) 1185, 1985, 2520
XcmI CCANNNNNNNNNTGG 1 cut(s) 1931
XhoI CTCGAG 2 cut(s) 23, 2888
XmiI GTMKAC 1 cut(s) 2680
XmnI GAANNNNTTC 3 cut(s) 594, 1272, 1854
XspI CTAG 7 cut(s) 1614, 1860, 2064, 2283, 2475, 2612, 2940
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.