Rw5G010260

Protein BREAST CANCER SUSCEPTIBILITY 1 homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0008716
Physical Location & Seq
Forward (+)
11264060 .. 11269874
5815 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G010260.1

Sequence Viewer

Length: 3273 bp
ATGGGCAATCTGGCGCACCTCGAGAAAATGGGCAGAGAACTCAAATGCCCAATATGTTTGAGTCTGTTTAGTTCAGCCGTTTCGCTGAATTGCAATCATGTCTTCTGCAACTCTTGTATTGTGAAATCAATGAAATTGGGTTCCAATTGCCCTGTTTGTAAGATCCCATATCAGCGGAGAGAGGTACGCCCTGCTCCTCATATGGACAATTTGGTTAGTATTTACAAAAGCATGGAGGATGCTTCAGGTATTAACATGTTTATGACTCAAAGTGTGCCGTCAGCTAAATCATCAGATGGAAAGCAGCAAGCTGAAGATGAGAACCCTGATGATCAAGATCCTCCTAAACTTTCTCAGAATAGAGCTGGGAACGGGAGAACCGTAAGAGGGAAAAGATCATGGAAAACTAAATCCAATCTAAAGAACTCTGAATCTGTTTCAGTTAAGCCTTCATTTCCAACCAAGAAAAGGGTTCAGGTTCCACAATGTCCTCCTTCAGAAACCCCTACACGGCCTAAACAGTTACCGGGTGGTTTTATTGATGAAAAGTCAAAGGAGGAGTCTACAACACTAAAGGAGAAGCCTGTTTTAAATGAAAAGGGAGAACCGGTGCTTTCACCTTTCTTTTGGTTGAGTGACAAAGATGCTGAAAATTTGAGTCAGCCTTCTAGTGGTGATCAGCTTAATGATATTCCATCTCCCAATGTTCCTACTTTCAGTGATATCAAGGAGTCAGATGATGAAGACTGTTCTAGATTGAGCCCACTGGGCAAAGTGCAGGGGAAATCTTCCAATGTTGCTGATTTTTTTGATAGTGAAATGTTCGAATGGACGCAAAGGCCGTGCTCCCCTGAGCTCTTTGCAAGTCCTTCTAAGATGCAGGTTGTGGATAGTGATCAAATTGATAGAATTCAAGTGAAGGAACTAAAAGAAGCTTTACTAAACAAGAAAATAGGTGAACAAGTCACTGCTGAAAAAGAAAGGTCCAGAAATTCTAGACAGAGTAGTGGAAACTTAATCTTGCTGCCTGATCTTCCACATGATAAAACTGAAGATGCTAATCTTCAATTTCTTTGCAAACCATCTAACAAGAAAGGCAGACAAGCAAGAAAAATAGCTAAAAGAAAGTGTGCTACAAGTCATATGGACCTGGTTGAGAATAGTAGTGCTAATTTGAAGGGTTCTGAAGATTTTTATCAAGAACACGGCTGCAAGAATAGTGACAGTTCTATGGCCAAGGCTGGCAAAAGGAATAAGAAAGCTCATCCGTCTCGGCTCGCAACTAAGCCAACATGTGAAAGTGTTTGCATCCTCTCTATTGGTGCTGAAAGGCAGAATGAAGGCAGTGCTGAGAAGGGGTTAACTGAATTACCTACTTCACTAGAAAAGAATGAAGGCAGTGATGAAGCTTTTGCCCAGGAGAAGGCTAAGAAAATATGTACGGAGATAAATGCCAAGAACCAGTGTCGCTCTGTAAGGTCAAGGAAACAAAAGGTGGTCTCTATGCCCAACATACTCGATGAGGTATCGGAAATCAAGAAGCAAGCAAATAAGAATACTACAACTGAGTCGTCCCATGTAACTGTCCCTACAGATGATAACAGGAAAGTCTCTAACGTCAAGAATGTATCCACAAAACTTGCTAGCGAAGCCAAGTCATCTGATCGAGAATTGAAATTCAACAAAAGGGTCAGAGTTTCCTCTGGTGATGACTCAAAGGATAAGGAGGTATCAGAGATCAAGAAGCAAGCAAATAAGAATGCTCCAACTGAGTTGCCCCGTAGAAATCTCCCTACAAATGTTAGCAGGGAACTATCAGGAGTCAGAAAATGTGCTAGAGAAGCCAAGTCATGTGATCGAAAAACTACCAAAAAGTTGAGAGTTTCCTTTGATGACAATTCAAAAGATGAGGCTGTTATTGAGATTCGAGAGGGTCAACATAATGTTAATGAAAATGGTAACCAACCCTCTGAGAAGAGCAAAGGCAATTCCAATGCTAGAATTTTTACTGACCGATCATCAATGCAGAAGCTTCCTACATTGAAGAATGATGTGGTTTTGAGGAGATGTGATGCAGTTACTAGTAACATTCAATGTGCCTTTTGTCTTTCATCAGAAGAATCAGAGGCATCAGGAGAAATGGTTCACTATTATAATGGCAAGCCTGTTGCCGCAGATCACAATGGAGGGTCCAAGGTTATACATTCACACAGGAACTGCACTGAATGGGCTCCCAACGTATATTTTGAAGATGATATTGCTATGAATCTTGAAGCTGAGCTAACTAGAAGTCGTAGAATTAAATGTTGTTGCTGTGGAATTAAAGGAGCATCTCTTGGGTGTTATGAGAAGAGTTGTCGTAAGAGCTTTCATGTTAGCTGTGCAAAGATGACTCCTGAATGTCGATGGGATTCTGATAATTTTGTTATGTTATGCCCCATTCATGCCTCTTCTAAGTTGCCAAATGAAAGTTCTGAATCTGAAGCAAGGAGGAAGAAGAGCAACCCTAGAAAACAACCTGGTGCTGATCATAATAAAGTTGCTGTTAAACAAGACAGCAATACCTGTCAGGATCTGAAATTTTGTGGATCATCCAAGAAATTGGTTCTCTGTTGTTCAACTCTCACAAATGCAGAGCAGGAATATGTTGCAGAATTTGAAAAATTATCTGGAGTTACAGTCTTAAAGAAGTGGGACTCTAGTATCACACATATTGTTGCGCCAATAGATGAAAATGGAGCATGCAGAAGAACCCTCAAAGTATTGTTGGGAATCTTGGAGGGGAAGTGGATACTGAGCATGGGATGGATTAAAGCTTGCATGGAAGCCATGAAACTAGTGAATGAGGAGCCTTATGAAATTAGCGTTGACATTTATGGAATCAGGGATGGCCCTCGACTTGGTAGACTAAGACTGCAGAACAAGGAACCAAAGCTTTTTGATGGGCTTAAGTTTTACTTCATGGGAGATTTTGTACCTTCATACAAGGGGTATCTACAAGACCTTGTAATAGCTGCTGGAGGAACTGTTTTGCACAGAAAGCCTGTTCCAGAGAGCCAGAAAGGGTCGTCTGGAAGTCCCCTCGAATGTCGAACCTTCATTATCTTTAGTCTTGAGCTACCTGATCAATGTCATCCTAGCAAGAAGGGTACCATTTTCAACCAAAGGCAGGCGGATGCAAAGTCTTTGGCAAGTTCTGCTGCAGCCAAGGTAGTAAGCAATTCATGGATTTTGAACTCTGTTGCAGCCTGCAAGTTACAAAATCTCTCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000151 GO:0000152 GO:0000724 GO:0000725 GO:0003674 GO:0003824 GO:0004842 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005886 GO:0006139 GO:0006259 GO:0006281 GO:0006302 GO:0006310 GO:0006355 GO:0006357 GO:0006464 GO:0006725 GO:0006807 GO:0006950 GO:0006974 GO:0008150 GO:0008152 GO:0009314 GO:0009628 GO:0009889 GO:0009890 GO:0009891 GO:0009892 GO:0009893 GO:0009987 GO:0010212 GO:0010332 GO:0010468 GO:0010556 GO:0010557 GO:0010564 GO:0010565 GO:0010604 GO:0010605 GO:0010628 GO:0010638 GO:0010639 GO:0016020 GO:0016567 GO:0016740 GO:0019216 GO:0019217 GO:0019219 GO:0019222 GO:0019538 GO:0019787 GO:0031056 GO:0031057 GO:0031058 GO:0031323 GO:0031324 GO:0031325 GO:0031326 GO:0031327 GO:0031328 GO:0031399 GO:0031400 GO:0031401 GO:0031436 GO:0032268 GO:0032269 GO:0032270 GO:0032446 GO:0032991 GO:0033043 GO:0033044 GO:0033554 GO:0034641 GO:0035065 GO:0035066 GO:0035067 GO:0036211 GO:0042127 GO:0042304 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044464 GO:0045717 GO:0045787 GO:0045833 GO:0045893 GO:0045922 GO:0045935 GO:0045944 GO:0046483 GO:0046890 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0050789 GO:0050794 GO:0050896 GO:0051055 GO:0051128 GO:0051129 GO:0051130 GO:0051171 GO:0051172 GO:0051173 GO:0051246 GO:0051247 GO:0051248 GO:0051252 GO:0051254 GO:0051716 GO:0051726 GO:0060255 GO:0062012 GO:0062014 GO:0065007 GO:0070531 GO:0070647 GO:0071156 GO:0071158 GO:0071214 GO:0071478 GO:0071479 GO:0071480 GO:0071704 GO:0071944 GO:0080090 GO:0090068 GO:0090304 GO:0104004 GO:0140096 GO:1901360 GO:1901564 GO:1901983 GO:1901984 GO:1901985 GO:1902275 GO:1902494 GO:1902680 GO:1903506 GO:1903508 GO:1905268 GO:1905269 GO:1990234 GO:2000112 GO:2000756 GO:2000757 GO:2000758 GO:2001141 GO:2001251 GO:2001252
KEGG Pathways
Metabolic & Signaling
ko03440 map03440
Pfam Domains
Protein Families
BRCT BRCT_2 DYW_deaminase PPR zf-C3HC4_2 zf-HC5HC2H zf-RING_2

Protein Analysis

1090

Amino Acids

121.0

Weight (kDa)

8.96

Isoelectric Point (pI)

52.36

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-RING_6 PF14835 4 - 69 1.7e-06 zf-RING of BARD1-type protein
zf-C3HC4_3 PF13920 13 - 59 1.5e-06 Zinc finger, C3HC4 type (RING finger)
zf-C3HC4_2 PF13923 15 - 53 3e-10 Zinc finger, C3HC4 type (RING finger)
zf-RING_2 PF13639 15 - 54 2.8e-07 Ring finger domain
zf-RING_UBOX PF13445 16 - 48 8.4e-07 RING-type zinc-finger
zf-C3HC4 PF00097 16 - 53 1.3e-07 Zinc finger, C3HC4 type (RING finger)
zf-C3HC4_4 PF15227 16 - 53 4.5e-06 zinc finger of C3HC4-type, RING
zf-HC5HC2H_2 PF13832 735 - 797 9.1e-06 PHD-zinc-finger like domain
zf-HC5HC2H PF13771 737 - 815 3.8e-09 PHD-like zinc-binding domain
BRCT PF00533 867 - 941 1.7e-07 BRCA1 C Terminus (BRCT) domain
BRCT_2 PF16589 978 - 1089 1.8e-07 BRCT domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 2154
Acc36I ACCTGC 1 cut(s) 871
Acc65I GGTACC 1 cut(s) 3149
AccB1I GGYRCC 1 cut(s) 3149
AccI GTMKAC 2 cut(s) 563, 2905
AciI CCGC 3 cut(s) 175, 2172, 3173
AclWI GGATC 4 cut(s) 157, 332, 2580, 2596
AcoI YGGCCR 1 cut(s) 1233
AcsI RAATTY 7 cut(s) 652, 909, 991, 1676, 2001, 2579, 2654
AcuI CTGAAG 6 cut(s) 228, 333, 480, 1071, 1206, 2502
AfaI GTAC 4 cut(s) 186, 1441, 2976, 3151
AfiI CCNNNNNNNGG 7 cut(s) 387, 468, 510, 671, 1423, 2900, 3169
AflII CTTAAG 1 cut(s) 2948
AflIII ACRYGT 2 cut(s) 255, 1292
AgeI ACCGGT 1 cut(s) 607
AhlI ACTAGT 2 cut(s) 2081, 2836
AjnI CCWGG 3 cut(s) 1149, 1416, 2518
AjuI GAANNNNNNNTTGG 2 cut(s) 2455, 2487
AloI GAACNNNNNNTCC 2 cut(s) 2127, 2159
Alw21I GWGCWC 2 cut(s) 848, 858
Alw26I GTCTC 3 cut(s) 1275, 1504, 1615
AlwI GGATC 4 cut(s) 157, 332, 2580, 2596
AlwNI CAGNNNCTG 2 cut(s) 2217, 2575
Ama87I CYCGRG 1 cut(s) 20
AoxI GGCC 4 cut(s) 512, 839, 1233, 2890
ApeKI GCWGC 7 cut(s) 304, 1024, 1209, 3014, 3200, 3203, 3245
ApoI RAATTY 7 cut(s) 652, 909, 991, 1676, 2001, 2579, 2654
ArsI GACNNNNNNTTYG 2 cut(s) 545, 577
AsiGI ACCGGT 1 cut(s) 607
Asp700I GAANNNNTTC 1 cut(s) 2142
Asp718I GGTACC 1 cut(s) 3149
AspLEI GCGC 2 cut(s) 16, 2722
AspS9I GGNCC 4 cut(s) 984, 1147, 2190, 2891
AsuC2I CCSGG 1 cut(s) 528
AsuHPI GGTGA 4 cut(s) 609, 686, 968, 1718
AsuII TTCGAA 1 cut(s) 825
AsuNHI GCTAGC 1 cut(s) 1643
AvaI CYCGRG 1 cut(s) 20
AvaII GGWCC 3 cut(s) 984, 1147, 2190
BaeI ACNNNNGTAYC 2 cut(s) 2686, 2719
BalI TGGCCA 1 cut(s) 1235
BanI GGYRCC 1 cut(s) 3149
BanII GRGCYC 3 cut(s) 764, 858, 2233
BbsI GAAGAC 2 cut(s) 94, 750
Bbv12I GWGCWC 2 cut(s) 848, 858
BbvI GCAGC 7 cut(s) 316, 1011, 1196, 3001, 3187, 3215, 3257
BccI CCATC 7 cut(s) 290, 703, 1090, 2400, 2799, 2882, 2936
BceAI ACGGC 5 cut(s) 62, 262, 527, 826, 1222
BciT130I CCWGG 3 cut(s) 1151, 1418, 2520
BciVI GTATCC 2 cut(s) 1639, 2784
BclI TGATCA 5 cut(s) 331, 676, 895, 2527, 3124
BcnI CCSGG 1 cut(s) 528
BcoDI GTCTC 3 cut(s) 1275, 1504, 1615
BcuI ACTAGT 2 cut(s) 2081, 2836
BfmI CTRYAG 3 cut(s) 1590, 2915, 3201
BfrI CTTAAG 1 cut(s) 2948
BfuAI ACCTGC 1 cut(s) 871
BfuI GTATCC 2 cut(s) 1639, 2784
BglI GCCNNNNNGGC 1 cut(s) 768
BisI GCNGC 8 cut(s) 305, 1025, 1210, 2172, 3015, 3201, 3204, 3246
BlpI GCTNAGC 1 cut(s) 2277
BlsI GCNGC 8 cut(s) 306, 1026, 1211, 2173, 3016, 3202, 3205, 3247
Bme1390I CCNGG 4 cut(s) 528, 1151, 1418, 2520
Bme18I GGWCC 3 cut(s) 984, 1147, 2190
BmeT110I CYCGRG 1 cut(s) 20
BmgT120I GGNCC 4 cut(s) 984, 1147, 2190, 2891
BmiI GGNNCC 7 cut(s) 142, 480, 2191, 2232, 2850, 2928, 3151
BmrFI CCNGG 4 cut(s) 528, 1151, 1418, 2520
BmrI ACTGGG 1 cut(s) 776
BmsI GCATC 9 cut(s) 229, 634, 867, 1045, 1317, 2062, 2138, 2339, 3166
BmtI GCTAGC 1 cut(s) 1647
BmuI ACTGGG 1 cut(s) 776
BpiI GAAGAC 2 cut(s) 94, 750
BpmI CTGGAG 2 cut(s) 2691, 3039
Bpu10I CCTNAGC 1 cut(s) 852
Bpu1102I GCTNAGC 1 cut(s) 2277
Bpu14I TTCGAA 1 cut(s) 825
BpuEI CTTGAG 1 cut(s) 3134
BpuMI CCSGG 1 cut(s) 528
BsaBI GATNNNNATC 4 cut(s) 336, 894, 1059, 1194
BsaI GGTCTC 1 cut(s) 1504
BsaJI CCNNGG 4 cut(s) 1236, 1416, 2193, 3207
BsaWI WCCGGW 1 cut(s) 607
Bsc4I CCNNNNNNNGG 7 cut(s) 387, 468, 510, 671, 1423, 2900, 3169
Bse118I RCCGGY 1 cut(s) 607
Bse1I ACTGG 2 cut(s) 771, 1462
Bse8I GATNNNNATC 4 cut(s) 336, 894, 1059, 1194
BseBI CCWGG 3 cut(s) 1151, 1418, 2520
BseDI CCNNGG 4 cut(s) 1236, 1416, 2193, 3207
BseGI GGATG 8 cut(s) 244, 1264, 1308, 2591, 2810, 2893, 3133, 3181
BseJI GATNNNNATC 4 cut(s) 336, 894, 1059, 1194
BseLI CCNNNNNNNGG 7 cut(s) 387, 468, 510, 671, 1423, 2900, 3169
BseMII CTCAG 8 cut(s) 368, 843, 1341, 1557, 1761, 1962, 2268, 2786
BseNI ACTGG 2 cut(s) 771, 1462
BseRI GAGGAG 4 cut(s) 186, 572, 2077, 2861
BseXI GCAGC 7 cut(s) 316, 1011, 1196, 3001, 3187, 3215, 3257
BseYI CCCAGC 1 cut(s) 365
BsgI GTGCAG 2 cut(s) 797, 2203
BshFI GGCC 4 cut(s) 514, 841, 1235, 2892
BshNI GGYRCC 1 cut(s) 3149
BshTI ACCGGT 1 cut(s) 607
BsiHKAI GWGCWC 2 cut(s) 848, 858
BsiHKCI CYCGRG 1 cut(s) 20
BsiSI CCGG 2 cut(s) 527, 608
BslFI GGGAC 4 cut(s) 1558, 1571, 2708, 3063
BslI CCNNNNNNNGG 7 cut(s) 387, 468, 510, 671, 1423, 2900, 3169
BsmAI GTCTC 3 cut(s) 1275, 1504, 1615
BsmBI CGTCTC 1 cut(s) 1275
BsmFI GGGAC 4 cut(s) 1558, 1571, 2708, 3063
BsmI GAATGC 1 cut(s) 1765
BsnI GGCC 4 cut(s) 514, 841, 1235, 2892
Bso31I GGTCTC 1 cut(s) 1504
BsoBI CYCGRG 1 cut(s) 20
Bsp119I TTCGAA 1 cut(s) 825
Bsp1286I GDGCHC 4 cut(s) 764, 848, 858, 2233
Bsp1720I GCTNAGC 1 cut(s) 2277
BspACI CCGC 3 cut(s) 175, 2172, 3173
BspANI GGCC 4 cut(s) 514, 841, 1235, 2892
BspCNI CTCAG 8 cut(s) 367, 844, 1342, 1558, 1762, 1963, 2269, 2787
BspLI GGNNCC 7 cut(s) 142, 480, 2191, 2232, 2850, 2928, 3151
BspMAI CTGCAG 2 cut(s) 2919, 3205
BspMI ACCTGC 1 cut(s) 871
BspOI GCTAGC 1 cut(s) 1647
BspPI GGATC 4 cut(s) 157, 332, 2580, 2596
BspQI GCTCTTC 2 cut(s) 1970, 2492
BspT104I TTCGAA 1 cut(s) 825
BspT107I GGYRCC 1 cut(s) 3149
BspTI CTTAAG 1 cut(s) 2948
BspTNI GGTCTC 1 cut(s) 1504
BsrFI RCCGGY 1 cut(s) 607
BsrI ACTGG 2 cut(s) 771, 1462
BssAI RCCGGY 1 cut(s) 607
BssECI CCNNGG 4 cut(s) 1236, 1416, 2193, 3207
BssT1I CCWWGG 3 cut(s) 1236, 2193, 3207
Bst2UI CCWGG 3 cut(s) 1151, 1418, 2520
Bst4CI ACNGT 7 cut(s) 382, 522, 749, 1226, 1585, 2680, 3028
Bst6I CTCTTC 4 cut(s) 1970, 2345, 2455, 2492
BstAFI CTTAAG 1 cut(s) 2948
BstAPI GCANNNNNTGC 1 cut(s) 3197
BstBI TTCGAA 1 cut(s) 825
BstEII GGTNACC 1 cut(s) 1958
BstF5I GGATG 8 cut(s) 244, 1264, 1308, 2591, 2810, 2893, 3133, 3181
BstHHI GCGC 2 cut(s) 16, 2722
BstMAI GTCTC 3 cut(s) 1275, 1504, 1615
BstMWI GCNNNNNNNGC 5 cut(s) 768, 1649, 1841, 3040, 3197
BstNI CCWGG 3 cut(s) 1151, 1418, 2520
BstNSI RCATGY 3 cut(s) 259, 1296, 2745
BstPI GGTNACC 1 cut(s) 1958
BstSCI CCNGG 4 cut(s) 526, 1149, 1416, 2518
BstSFI CTRYAG 3 cut(s) 1590, 2915, 3201
BstV1I GCAGC 7 cut(s) 316, 1011, 1196, 3001, 3187, 3215, 3257
BstV2I GAAGAC 2 cut(s) 94, 750
BstX2I RGATCY 3 cut(s) 162, 337, 2572
BstXI CCANNNNNNTGG 1 cut(s) 2602
BstYI RGATCY 3 cut(s) 162, 337, 2572
BsuI GTATCC 2 cut(s) 1639, 2784
BsuRI GGCC 4 cut(s) 514, 841, 1235, 2892
BtsCI GGATG 8 cut(s) 244, 1264, 1308, 2591, 2810, 2893, 3133, 3181
BtsI GCAGTG 3 cut(s) 966, 1351, 1405
BtsIMutI CAGTG 7 cut(s) 724, 764, 966, 1351, 1405, 1469, 2220
BveI ACCTGC 1 cut(s) 871
CaiI CAGNNNCTG 2 cut(s) 2217, 2575
CfoI GCGC 2 cut(s) 16, 2722
Cfr10I RCCGGY 1 cut(s) 607
Cfr13I GGNCC 4 cut(s) 984, 1147, 2190, 2891
CseI GACGC 1 cut(s) 841
CsiI ACCWGGT 2 cut(s) 1149, 2518
Csp6I GTAC 4 cut(s) 185, 1440, 2975, 3150
CspAI ACCGGT 1 cut(s) 607
CspCI CAANNNNNGTGG 2 cut(s) 1621, 1656
CviQI GTAC 4 cut(s) 185, 1440, 2975, 3150
DraI TTTAAA 1 cut(s) 591
EaeI YGGCCR 1 cut(s) 1233
Eam1104I CTCTTC 4 cut(s) 1970, 2345, 2455, 2492
EarI CTCTTC 4 cut(s) 1970, 2345, 2455, 2492
EciI GGCGGA 1 cut(s) 3188
Ecl136II GAGCTC 1 cut(s) 856
Eco130I CCWWGG 3 cut(s) 1236, 2193, 3207
Eco24I GRGCYC 3 cut(s) 764, 858, 2233
Eco31I GGTCTC 1 cut(s) 1504
Eco32I GATATC 1 cut(s) 724
Eco47I GGWCC 3 cut(s) 984, 1147, 2190
Eco53kI GAGCTC 1 cut(s) 856
Eco57I CTGAAG 6 cut(s) 228, 333, 480, 1071, 1206, 2502
Eco88I CYCGRG 1 cut(s) 20
Eco91I GGTNACC 1 cut(s) 1958
EcoICRI GAGCTC 1 cut(s) 856
EcoO65I GGTNACC 1 cut(s) 1958
EcoRI GAATTC 1 cut(s) 909
EcoRII CCWGG 3 cut(s) 1149, 1416, 2518
EcoRV GATATC 1 cut(s) 724
EcoT14I CCWWGG 3 cut(s) 1236, 2193, 3207
EcoT38I GRGCYC 3 cut(s) 764, 858, 2233
ErhI CCWWGG 3 cut(s) 1236, 2193, 3207
Esp3I CGTCTC 1 cut(s) 1275
FalI AAGNNNNNCTT 2 cut(s) 2942, 2974
FaqI GGGAC 4 cut(s) 1558, 1571, 2708, 3063
FauNDI CATATG 2 cut(s) 201, 1143
FbaI TGATCA 5 cut(s) 331, 676, 895, 2527, 3124
FblI GTMKAC 2 cut(s) 563, 2905
Fnu4HI GCNGC 8 cut(s) 305, 1025, 1210, 2172, 3015, 3201, 3204, 3246
FokI GGATG 8 cut(s) 251, 1251, 1295, 2578, 2817, 2900, 3120, 3188
FriOI GRGCYC 3 cut(s) 764, 858, 2233
Fsp4HI GCNGC 8 cut(s) 305, 1025, 1210, 2172, 3015, 3201, 3204, 3246
GlaI GCGC 2 cut(s) 15, 2721
GluI GCNGC 8 cut(s) 305, 1025, 1210, 2172, 3015, 3201, 3204, 3246
GsaI CCCAGC 1 cut(s) 369
GsuI CTGGAG 2 cut(s) 2691, 3039
HaeIII GGCC 4 cut(s) 514, 841, 1235, 2892
HapII CCGG 2 cut(s) 527, 608
HgaI GACGC 1 cut(s) 841
HhaI GCGC 2 cut(s) 16, 2722
Hin6I GCGC 2 cut(s) 14, 2720
HinP1I GCGC 2 cut(s) 14, 2720
HincII GTYRAC 3 cut(s) 1362, 1937, 2869
HindII GTYRAC 3 cut(s) 1362, 1937, 2869
HindIII AAGCTT 5 cut(s) 933, 1407, 2030, 2814, 2933
HpaI GTTAAC 1 cut(s) 1362
HpaII CCGG 2 cut(s) 527, 608
HphI GGTGA 4 cut(s) 609, 686, 968, 1718
Hpy166II GTNNAC 7 cut(s) 564, 959, 1362, 1937, 2146, 2869, 2906
Hpy8I GTNNAC 7 cut(s) 564, 959, 1362, 1937, 2146, 2869, 2906
HpyCH4III ACNGT 7 cut(s) 382, 522, 749, 1226, 1585, 2680, 3028
HpyCH4IV ACGT 2 cut(s) 1617, 2238
HpyF10VI GCNNNNNNNGC 5 cut(s) 768, 1649, 1841, 3040, 3197
HpySE526I ACGT 2 cut(s) 1617, 2238
HspAI GCGC 2 cut(s) 14, 2720
KpnI GGTACC 1 cut(s) 3153
Ksp22I TGATCA 5 cut(s) 331, 676, 895, 2527, 3124
KspAI GTTAAC 1 cut(s) 1362
LguI GCTCTTC 2 cut(s) 1970, 2492
LmnI GCTCC 7 cut(s) 199, 851, 1768, 2236, 2327, 2738, 2848
Lsp1109I GCAGC 7 cut(s) 316, 1011, 1196, 3001, 3187, 3215, 3257
LweI GCATC 9 cut(s) 229, 634, 867, 1045, 1317, 2062, 2138, 2339, 3166
MabI ACCWGGT 2 cut(s) 1149, 2518
MaeII ACGT 2 cut(s) 1617, 2238
MfeI CAATTG 1 cut(s) 145
MflI RGATCY 3 cut(s) 162, 337, 2572
MhlI GDGCHC 4 cut(s) 764, 848, 858, 2233
MlsI TGGCCA 1 cut(s) 1235
MluNI TGGCCA 1 cut(s) 1235
MmeI TCCRAC 2 cut(s) 482, 1790
Mox20I TGGCCA 1 cut(s) 1235
MroXI GAANNNNTTC 1 cut(s) 2142
MscI TGGCCA 1 cut(s) 1235
MslI CAYNNNNRTG 1 cut(s) 260
Msp20I TGGCCA 1 cut(s) 1235
MspA1I CMGCKG 1 cut(s) 175
MspCI CTTAAG 1 cut(s) 2948
MspI CCGG 2 cut(s) 527, 608
MspR9I CCNGG 4 cut(s) 528, 1151, 1418, 2520
MunI CAATTG 1 cut(s) 145
Mva1269I GAATGC 1 cut(s) 1765
MvaI CCWGG 3 cut(s) 1151, 1418, 2520
MwoI GCNNNNNNNGC 5 cut(s) 768, 1649, 1841, 3040, 3197
NciI CCSGG 1 cut(s) 528
NdeI CATATG 2 cut(s) 201, 1143
NheI GCTAGC 1 cut(s) 1643
NlaIV GGNNCC 7 cut(s) 142, 480, 2191, 2232, 2850, 2928, 3151
NmeAIII GCCGAG 1 cut(s) 1252
NmuCI GTSAC 3 cut(s) 635, 964, 1220
NspI RCATGY 3 cut(s) 259, 1296, 2745
NspV TTCGAA 1 cut(s) 825
PaeI GCATGC 1 cut(s) 2745
PaeR7I CTCGAG 1 cut(s) 20
PciI ACATGT 2 cut(s) 255, 1292
PciSI GCTCTTC 2 cut(s) 1970, 2492
PcsI WCGNNNNNNNCGW 2 cut(s) 378, 839
PctI GAATGC 1 cut(s) 1765
PdmI GAANNNNTTC 1 cut(s) 2142
PfeI GAWTC 8 cut(s) 431, 1924, 2120, 2266, 2411, 2477, 2772, 2880
PinAI ACCGGT 1 cut(s) 607
PkrI GCNGC 8 cut(s) 306, 1026, 1211, 2173, 3016, 3202, 3205, 3247
PscI ACATGT 2 cut(s) 255, 1292
PsiI TTATAA 1 cut(s) 2154
Psp124BI GAGCTC 1 cut(s) 858
Psp6I CCWGG 3 cut(s) 1149, 1416, 2518
PspEI GGTNACC 1 cut(s) 1958
PspFI CCCAGC 1 cut(s) 365
PspGI CCWGG 3 cut(s) 1149, 1416, 2518
PspN4I GGNNCC 7 cut(s) 142, 480, 2191, 2232, 2850, 2928, 3151
PspPI GGNCC 4 cut(s) 984, 1147, 2190, 2891
PstI CTGCAG 2 cut(s) 2919, 3205
PstNI CAGNNNCTG 2 cut(s) 2217, 2575
PsuI RGATCY 3 cut(s) 162, 337, 2572
RsaI GTAC 4 cut(s) 186, 1441, 2976, 3151
RsaNI GTAC 4 cut(s) 185, 1440, 2975, 3150
RseI CAYNNNNRTG 1 cut(s) 260
SacI GAGCTC 1 cut(s) 858
SapI GCTCTTC 2 cut(s) 1970, 2492
SatI GCNGC 8 cut(s) 305, 1025, 1210, 2172, 3015, 3201, 3204, 3246
Sau96I GGNCC 4 cut(s) 984, 1147, 2190, 2891
ScrFI CCNGG 4 cut(s) 528, 1151, 1418, 2520
SduI GDGCHC 4 cut(s) 764, 848, 858, 2233
SexAI ACCWGGT 2 cut(s) 1149, 2518
SfaNI GCATC 9 cut(s) 229, 634, 867, 1045, 1317, 2062, 2138, 2339, 3166
SfcI CTRYAG 3 cut(s) 1590, 2915, 3201
Sfr274I CTCGAG 1 cut(s) 20
SfuI TTCGAA 1 cut(s) 825
SinI GGWCC 3 cut(s) 984, 1147, 2190
SlaI CTCGAG 1 cut(s) 20
SmiMI CAYNNNNRTG 1 cut(s) 260
SmlI CTYRAG 3 cut(s) 20, 2948, 3113
SmoI CTYRAG 3 cut(s) 20, 2948, 3113
SpeI ACTAGT 2 cut(s) 2081, 2836
SphI GCATGC 1 cut(s) 2745
SsiI CCGC 3 cut(s) 175, 2172, 3173
SstI GAGCTC 1 cut(s) 858
StyD4I CCNGG 4 cut(s) 526, 1149, 1416, 2518
StyI CCWWGG 3 cut(s) 1236, 2193, 3207
TaaI ACNGT 7 cut(s) 382, 522, 749, 1226, 1585, 2680, 3028
TaiI ACGT 2 cut(s) 1620, 2241
TaqII GACCGA 1 cut(s) 2028
TauI GCSGC 1 cut(s) 2174
TfiI GAWTC 8 cut(s) 431, 1924, 2120, 2266, 2411, 2477, 2772, 2880
TscAI CASTG 7 cut(s) 724, 771, 973, 1351, 1405, 1469, 2227
TseFI GTSAC 3 cut(s) 635, 964, 1220
TseI GCWGC 7 cut(s) 304, 1024, 1209, 3014, 3200, 3203, 3245
Tsp45I GTSAC 3 cut(s) 635, 964, 1220
TspGWI ACGGA 2 cut(s) 1257, 1457
TspRI CASTG 7 cut(s) 724, 771, 973, 1351, 1405, 1469, 2227
Vha464I CTTAAG 1 cut(s) 2948
VpaK11BI GGWCC 3 cut(s) 984, 1147, 2190
XapI RAATTY 7 cut(s) 652, 909, 991, 1676, 2001, 2579, 2654
XbaI TCTAGA 2 cut(s) 752, 995
XceI RCATGY 3 cut(s) 259, 1296, 2745
XhoI CTCGAG 1 cut(s) 20
XmiI GTMKAC 2 cut(s) 563, 2905
XmnI GAANNNNTTC 1 cut(s) 2142
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.