pycom05g25830

Protein BREAST CANCER SUSCEPTIBILITY 1 homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr5
OG0008716
Physical Location & Seq
Reverse (-)
27150557 .. 27155754
5198 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom05g25830.5

Sequence Viewer

Length: 3069 bp
ATGGAGACTCCTCCGCCCCACCTCGAGAAAATGGGCAGAGAACTCAAGTGCCCCATCTGCGCTTGCATTGTGAAGTCGATGAAATCGGGTTCCAATTGTCCTGTTTGTAAAATTCCATATCGGCGTAGGGGGTACGATTGTTTTATGTGCAATCCATCATTCTTATCAATAATTTTTGTTTTAGAGGTTCGCCCGGCTCCTCACATGGATAATTTGGTTAGTATTTACAAGAGCATGGAAGATGCTTCGGGAATTAATATATTTGTCACTCAGAATGCACCCTCAACTAAATCATCAGACGGAAAACAGCAAGCTGAAGATGATTTTAGCCATGGCGAGCATGATCTTCCCGAGTTTCAGGATAGAGCTCAGAAACAGAAAACCATAAGAGGAAAAGGATCCAGGAATGCTAAGGCCAACCTGAAAAGTTCTGGTTCTATTTCAATCAAACCTTCATTTCCAAACAAGAAAAGGGTTCAGGTTCCACAGTGTCCTATTTCAGAAACCCCAACTCGCCATCAAAAGTTAGAAGGTGATTTAATGGCTGATCGAGCTAAGCAGAGTTCAAAAACACTAAAGGAGAAGCCTGTTCTAAATGAAAAGGGAGAACCGGTGCTGTCACCTTTCTTTTGGTTGAATGACAAAGATGTAGAAAGTTTGAGTCAGTTTTCTGTCGGTGATCAACTTACCGATCTTCCATCTCCAAATGTTCCTGCATTCAGTGATATCAAAGACTCAGATGATGAAAACTCTGTTAGATTGACCCAACCGGGGGAAGTGCACGGTACATCTTCTCATGCTGCAGATTTATTTGATAGTGAGATTTTTGAATGGACACAAATACCTTGCTCTCCTGAACTCTTCCCAAGTCCTCCTAAGATGCAGGTTGGAGAGGATGACAACGATAGAGTTCAATTGAAGGAACTAAAAGAAGTTTCACAAAACAAGAAATCAGTTGAGCTTTCTGCTAAAAGTGCAAGGTCTAAAAGAAACAAAAAAAGTACTGGAAATATGGATTTTCTGCCAGATCTTCCAGCTGCTGGAACTAAAGCTGTTACTAATGAATCTGACAAGCGAGGTAGGAAGGCAAAGAATCTCACCAAAAGAAAGTGTGCTACAAGTCATACAGATCCAGGAACAGATATTAATGTCACTTTGAAGGGATCTGAGGTATTTTATGAAGGACAAGCATGTGAGAATTACGGTAGTTCTTTGGCCAAGACTCGCAAAAGAAGTAAGAAGGAACATTCATCTGTTGTTGTAACCAAGCCCACATCTGAAAATGTTCATGTCCTCTCTACCAGAGCTGATAGTCGAATTAATGGTGAAGAAAATGGGATAACTGCATCACCTACTTCATTGGGAAAGAATGCAGGCGGTGATGAACCATCCAGTAAGAAAGCTGGAAAAATGTGTAGGGAGGTCAATGACAAGAACAAGATTGACCGCCCAGTGAGGTCAAAAAAACAAAAGATGGTCTCCATGCAAAAGACAATGCTTGAGGCATCAGAAATACAGAAGCAAGCAAATAAGGATACTCCTACTGAGTTGTCTCTTATCAATCTTCCTACAGATGATAACCAGAAAGCCTCTGAATCCTGGAATAATTCCAGAAAATTTGCTAGAACAGCCAAGTTGGGTGATCAAGAACTGAAAAGGAACAAAAAGGTGAAAGTTTCTTCTGATGACAACTCAAAGGACGATACTTCTTTTGGGATTGGAGAAGGTCATGGGCATGTTAATGAAAATGGAAATCAACCTGCTGAGAAGAGCAAGGGAAATTGTAATGTTACAACTAATCAATCACCAGTGCAGAAGCTTCCTTCATTGACAAAGAATGTGGTCTTGAAGAGATGTGAAGCTATTCCTAGTAAAATCCAATGTGCTTTCTGTCTTTCATCAGAAGAATCAGAGGCTACTGGAGAGATAGGTCACTACTATAATGGCAAGCCTGTTGCTGCGGATCACAATGGAGGACTCAAGGTTATACATGCACACAGGATCTGCGCTGAATGGGCTCCCAATGTCTACTTTGAAGAAGATATTGCTATTAATCTTGAAGCTGAATTAACTAGGAGTCGAAAAATTAAATGTTCTTGCTGCGCAATTAAAGGAGCAGCTCTTGGGTGCTATGAGAAGAGTTGTCGTAGGAGCTTTCATGTCACCTGTGCAAAGTTGATGCCTCAATGTCTATGGGATACTGACAATTTTGTTATGTTATGCCCTCTTCATGCCTCCTCGAAGTTGCCAAATGAAAGTCCTGAATCTCAAGCAACGAGGAAGAAAAGCAATCCTAAAAAACAACCTAATGTTGAATGTCCTAAAGTTGCTGCTAAACAACACAGCAATACCCCTCCAGATCAGAAGTTTTGTGGACCGTCCAAGAAATTGGTTCTTTGTTGTTCATCCCTCACAAATGCAGAGAGGGAGTTTGTCTCTGGATTTGAAAGATTATCTGGACTTACGGTTTTGAAGAATTGGGATTCTAATGTTACACATGTTATTGCGTCGACAGATGAAAATGGAGCATGCAGAAGGACCCTCAAAGTTTTGATGGGTATCTTGGAGGGGAAGTGGATCCTGAATGTGGAGTGGATTAATGCTTGCACAGAGGCCATGAAACTAGTTGATGAGGAGCCTTATGAAATCAACGTCGACATTTATGGAATCAGGGATGGTCCTCGACTTGGTAGACTAAGGCTGCAGAACAAGCAACCAAAGCTTTTTGATGGATTCAAGTTTTACTTCATGGGAGACTTTAAGCCTTCATACAAGGGGTATCTACAAGACCTTGTAATAGCTGCCGGAGGAACGATTCTGCAAAGAAAGCCTGTTCCAGAGGGCCCAAAAGCCTTGTCAGCCGGTTCCCCCAAATGTCAAACCTACATAATTTACAGCCTCGAGCTACCCGATCAATGTCATCGTAGCAAGAAGAGTACGATTTTCAACACTAGGCAGGCCGATGCAAAGGCTTTGGCCAGTTCCGCTGTAGCTGTGGCAGTGAGCAATTCATGGGTTTTGAACTCCATTGCTGCCTGCAAGTTACAAAATCTTTCTGATTTCGAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000151 GO:0000152 GO:0000724 GO:0000725 GO:0003674 GO:0003824 GO:0004842 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005886 GO:0006139 GO:0006259 GO:0006281 GO:0006302 GO:0006310 GO:0006355 GO:0006357 GO:0006464 GO:0006725 GO:0006807 GO:0006950 GO:0006974 GO:0008150 GO:0008152 GO:0009314 GO:0009628 GO:0009889 GO:0009890 GO:0009891 GO:0009892 GO:0009893 GO:0009987 GO:0010212 GO:0010332 GO:0010468 GO:0010556 GO:0010557 GO:0010564 GO:0010565 GO:0010604 GO:0010605 GO:0010628 GO:0010638 GO:0010639 GO:0016020 GO:0016567 GO:0016740 GO:0019216 GO:0019217 GO:0019219 GO:0019222 GO:0019538 GO:0019787 GO:0031056 GO:0031057 GO:0031058 GO:0031323 GO:0031324 GO:0031325 GO:0031326 GO:0031327 GO:0031328 GO:0031399 GO:0031400 GO:0031401 GO:0031436 GO:0032268 GO:0032269 GO:0032270 GO:0032446 GO:0032991 GO:0033043 GO:0033044 GO:0033554 GO:0034641 GO:0035065 GO:0035066 GO:0035067 GO:0036211 GO:0042127 GO:0042304 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044464 GO:0045717 GO:0045787 GO:0045833 GO:0045893 GO:0045922 GO:0045935 GO:0045944 GO:0046483 GO:0046890 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0050789 GO:0050794 GO:0050896 GO:0051055 GO:0051128 GO:0051129 GO:0051130 GO:0051171 GO:0051172 GO:0051173 GO:0051246 GO:0051247 GO:0051248 GO:0051252 GO:0051254 GO:0051716 GO:0051726 GO:0060255 GO:0062012 GO:0062014 GO:0065007 GO:0070531 GO:0070647 GO:0071156 GO:0071158 GO:0071214 GO:0071478 GO:0071479 GO:0071480 GO:0071704 GO:0071944 GO:0080090 GO:0090068 GO:0090304 GO:0104004 GO:0140096 GO:1901360 GO:1901564 GO:1901983 GO:1901984 GO:1901985 GO:1902275 GO:1902494 GO:1902680 GO:1903506 GO:1903508 GO:1905268 GO:1905269 GO:1990234 GO:2000112 GO:2000756 GO:2000757 GO:2000758 GO:2001141 GO:2001251 GO:2001252
KEGG Pathways
Metabolic & Signaling
ko03440 map03440
Pfam Domains
Protein Families
BRCT BRCT_2 DYW_deaminase PPR zf-C3HC4_2 zf-HC5HC2H zf-RING_2

Protein Analysis

1023

Amino Acids

112.9

Weight (kDa)

8.93

Isoelectric Point (pI)

44.27

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 2104
Acc36I ACCTGC 2 cut(s) 874, 1768
AccB7I CCANNNNNTGG 1 cut(s) 1040
AccI GTMKAC 4 cut(s) 2028, 2510, 2655, 2692
AciI CCGC 5 cut(s) 14, 1377, 1447, 1961, 2985
AclWI GGATC 8 cut(s) 393, 406, 1124, 1171, 1971, 2009, 2572, 2585
AcoI YGGCCR 2 cut(s) 1215, 2976
AcsI RAATTY 2 cut(s) 111, 1616
AcuI CTGAAG 1 cut(s) 336
AfaI GTAC 4 cut(s) 134, 787, 1003, 2938
AfiI CCNNNNNNNGG 5 cut(s) 771, 772, 1040, 2587, 2687
AflIII ACRYGT 1 cut(s) 2497
AgeI ACCGGT 1 cut(s) 610
AhlI ACTAGT 1 cut(s) 2623
AjnI CCWGG 3 cut(s) 401, 1132, 1598
Alw21I GWGCWC 2 cut(s) 370, 783
Alw26I GTCTC 4 cut(s) 1483, 1557, 2440, 2748
Alw44I GTGCAC 1 cut(s) 779
AlwI GGATC 8 cut(s) 393, 406, 1124, 1171, 1971, 2009, 2572, 2585
AlwNI CAGNNNCTG 2 cut(s) 1040, 2004
Ama87I CYCGRG 3 cut(s) 23, 350, 2900
AoxI GGCC 6 cut(s) 414, 1215, 2613, 2842, 2958, 2976
ApaI GGGCCC 1 cut(s) 2846
ApaLI GTGCAC 1 cut(s) 779
ApeKI GCWGC 9 cut(s) 800, 1037, 1958, 2100, 2117, 2330, 2701, 2801, 3032
ApoI RAATTY 2 cut(s) 111, 1616
ArsI GACNNNNNNTTYG 2 cut(s) 2840, 2872
AseI ATTAAT 5 cut(s) 255, 1146, 1320, 2052, 2598
AsiGI ACCGGT 1 cut(s) 610
Asp700I GAANNNNTTC 2 cut(s) 1284, 1863
AspLEI GCGC 3 cut(s) 62, 2009, 2105
AspS9I GGNCC 5 cut(s) 2375, 2538, 2678, 2842, 2843
AsuC2I CCSGG 2 cut(s) 194, 771
AsuII TTCGAA 1 cut(s) 3063
AvaI CYCGRG 3 cut(s) 23, 350, 2900
AvaII GGWCC 3 cut(s) 2375, 2538, 2678
BaeGI GKGCMC 3 cut(s) 53, 783, 2846
BaeI ACNNNNGTAYC 2 cut(s) 124, 157
BalI TGGCCA 2 cut(s) 1217, 2978
BamHI GGATCC 2 cut(s) 398, 2577
BanII GRGCYC 3 cut(s) 370, 2020, 2846
Bbv12I GWGCWC 2 cut(s) 370, 783
BbvI GCAGC 9 cut(s) 787, 1024, 1945, 2087, 2129, 2317, 2688, 2788, 3019
BccI CCATC 9 cut(s) 62, 163, 525, 706, 1396, 1468, 2548, 2669, 2723
BciT130I CCWGG 3 cut(s) 403, 1134, 1600
BciVI GTATCC 2 cut(s) 1528, 2191
BclI TGATCA 2 cut(s) 679, 1642
BcnI CCSGG 2 cut(s) 194, 771
BcoDI GTCTC 4 cut(s) 1483, 1557, 2440, 2748
BcuI ACTAGT 1 cut(s) 2623
BfaI CTAG 5 cut(s) 1623, 1869, 2073, 2624, 2952
BfmI CTRYAG 4 cut(s) 801, 1569, 2702, 2988
BfuAI ACCTGC 2 cut(s) 874, 1768
BfuI GTATCC 2 cut(s) 1528, 2191
BglII AGATCT 1 cut(s) 1027
BisI GCNGC 9 cut(s) 801, 1038, 1959, 2101, 2118, 2331, 2702, 2802, 3033
BlpI GCTNAGC 1 cut(s) 555
BlsI GCNGC 9 cut(s) 802, 1039, 1960, 2102, 2119, 2332, 2703, 2803, 3034
BmcAI AGTACT 1 cut(s) 1003
Bme1390I CCNGG 5 cut(s) 194, 403, 771, 1134, 1600
Bme18I GGWCC 3 cut(s) 2375, 2538, 2678
BmeT110I CYCGRG 3 cut(s) 23, 350, 2900
BmgT120I GGNCC 5 cut(s) 2375, 2538, 2678, 2842, 2843
BmrFI CCNGG 5 cut(s) 194, 403, 771, 1134, 1600
BmrI ACTGGG 1 cut(s) 1445
BmsI GCATC 6 cut(s) 232, 870, 1355, 1514, 2169, 2953
BmuI ACTGGG 1 cut(s) 1445
BplI GAGNNNNNCTC 2 cut(s) 2420, 2452
BpmI CTGGAG 2 cut(s) 1941, 2340
Bpu10I CCTNAGC 1 cut(s) 411
Bpu1102I GCTNAGC 1 cut(s) 555
Bpu14I TTCGAA 1 cut(s) 3063
BpuEI CTTGAG 4 cut(s) 29, 1520, 1964, 2253
BpuMI CCSGG 2 cut(s) 194, 771
BsaBI GATNNNNATC 1 cut(s) 2558
BsaI GGTCTC 1 cut(s) 1483
BsaJI CCNNGG 2 cut(s) 331, 770
BsaWI WCCGGW 1 cut(s) 610
BsaXI ACNNNNNCTCC 4 cut(s) 597, 627, 2558, 2588
Bsc4I CCNNNNNNNGG 5 cut(s) 771, 772, 1040, 2587, 2687
Bse118I RCCGGY 2 cut(s) 610, 2861
Bse1I ACTGG 6 cut(s) 1009, 1392, 1451, 1808, 1924, 2979
Bse3DI GCAATG 1 cut(s) 3027
Bse8I GATNNNNATC 1 cut(s) 2558
BseBI CCWGG 3 cut(s) 403, 1134, 1600
BseDI CCNNGG 2 cut(s) 331, 770
BseGI GGATG 4 cut(s) 901, 1388, 2405, 2680
BseJI GATNNNNATC 1 cut(s) 2558
BseLI CCNNNNNNNGG 5 cut(s) 771, 772, 1040, 2587, 2687
BseMI GCAATG 1 cut(s) 3027
BseMII CTCAG 6 cut(s) 284, 383, 750, 1158, 1536, 1755
BseNI ACTGG 6 cut(s) 1009, 1392, 1451, 1808, 1924, 2979
BseRI GAGGAG 3 cut(s) 189, 2227, 2648
BseSI GKGCMC 3 cut(s) 53, 783, 2846
BseXI GCAGC 9 cut(s) 787, 1024, 1945, 2087, 2129, 2317, 2688, 2788, 3019
BsgI GTGCAG 1 cut(s) 1832
BshFI GGCC 6 cut(s) 416, 1217, 2615, 2844, 2960, 2978
BshTI ACCGGT 1 cut(s) 610
BsiHKAI GWGCWC 2 cut(s) 370, 783
BsiHKCI CYCGRG 3 cut(s) 23, 350, 2900
BsiSI CCGG 5 cut(s) 194, 611, 770, 2805, 2862
BslI CCNNNNNNNGG 5 cut(s) 771, 772, 1040, 2587, 2687
BsmAI GTCTC 4 cut(s) 1483, 1557, 2440, 2748
BsmI GAATGC 4 cut(s) 280, 412, 716, 1375
BsnI GGCC 6 cut(s) 416, 1217, 2615, 2844, 2960, 2978
Bso31I GGTCTC 1 cut(s) 1483
BsoBI CYCGRG 3 cut(s) 23, 350, 2900
Bsp119I TTCGAA 1 cut(s) 3063
Bsp120I GGGCCC 1 cut(s) 2842
Bsp1286I GDGCHC 5 cut(s) 53, 370, 783, 2020, 2846
Bsp1720I GCTNAGC 1 cut(s) 555
Bsp19I CCATGG 1 cut(s) 331
BspACI CCGC 5 cut(s) 14, 1377, 1447, 1961, 2985
BspANI GGCC 6 cut(s) 416, 1217, 2615, 2844, 2960, 2978
BspCNI CTCAG 6 cut(s) 283, 382, 749, 1159, 1537, 1756
BspMAI CTGCAG 2 cut(s) 805, 2706
BspMI ACCTGC 2 cut(s) 874, 1768
BspPI GGATC 8 cut(s) 393, 406, 1124, 1171, 1971, 2009, 2572, 2585
BspQI GCTCTTC 1 cut(s) 1763
BspT104I TTCGAA 1 cut(s) 3063
BspTNI GGTCTC 1 cut(s) 1483
BsrDI GCAATG 1 cut(s) 3027
BsrFI RCCGGY 2 cut(s) 610, 2861
BsrI ACTGG 6 cut(s) 1009, 1392, 1451, 1808, 1924, 2979
BssAI RCCGGY 2 cut(s) 610, 2861
BssECI CCNNGG 2 cut(s) 331, 770
BssT1I CCWWGG 1 cut(s) 331
Bst2UI CCWGG 3 cut(s) 403, 1134, 1600
Bst4CI ACNGT 5 cut(s) 489, 785, 1205, 2379, 2467
Bst6I CTCTTC 6 cut(s) 866, 1763, 1844, 2132, 2232, 2927
BstBI TTCGAA 1 cut(s) 3063
BstDSI CCRYGG 1 cut(s) 331
BstF5I GGATG 4 cut(s) 901, 1388, 2405, 2680
BstHHI GCGC 3 cut(s) 62, 2009, 2105
BstMAI GTCTC 4 cut(s) 1483, 1557, 2440, 2748
BstNI CCWGG 3 cut(s) 403, 1134, 1600
BstNSI RCATGY 5 cut(s) 1194, 1739, 1994, 2501, 2532
BstSCI CCNGG 5 cut(s) 192, 401, 769, 1132, 1598
BstSFI CTRYAG 4 cut(s) 801, 1569, 2702, 2988
BstSLI GKGCMC 3 cut(s) 53, 783, 2846
BstV1I GCAGC 9 cut(s) 787, 1024, 1945, 2087, 2129, 2317, 2688, 2788, 3019
BstX2I RGATCY 6 cut(s) 398, 1027, 1129, 1163, 2001, 2577
BstXI CCANNNNNNTGG 1 cut(s) 2389
BstYI RGATCY 6 cut(s) 398, 1027, 1129, 1163, 2001, 2577
BsuI GTATCC 2 cut(s) 1528, 2191
BsuRI GGCC 6 cut(s) 416, 1217, 2615, 2844, 2960, 2978
BtgI CCRYGG 1 cut(s) 331
BtsCI GGATG 4 cut(s) 901, 1388, 2405, 2680
BtsI GCAGTG 1 cut(s) 3006
BtsIMutI CAGTG 5 cut(s) 494, 727, 1458, 1815, 3006
BveI ACCTGC 2 cut(s) 874, 1768
CaiI CAGNNNCTG 2 cut(s) 1040, 2004
CfoI GCGC 3 cut(s) 62, 2009, 2105
Cfr10I RCCGGY 2 cut(s) 610, 2861
Cfr13I GGNCC 5 cut(s) 2375, 2538, 2678, 2842, 2843
CseI GACGC 1 cut(s) 2496
Csp6I GTAC 4 cut(s) 133, 786, 1002, 2937
CspAI ACCGGT 1 cut(s) 610
CspCI CAANNNNNGTGG 2 cut(s) 1821, 1856
CviQI GTAC 4 cut(s) 133, 786, 1002, 2937
EaeI YGGCCR 2 cut(s) 1215, 2976
Eam1104I CTCTTC 6 cut(s) 866, 1763, 1844, 2132, 2232, 2927
EarI CTCTTC 6 cut(s) 866, 1763, 1844, 2132, 2232, 2927
Ecl136II GAGCTC 1 cut(s) 368
Eco130I CCWWGG 1 cut(s) 331
Eco24I GRGCYC 3 cut(s) 370, 2020, 2846
Eco31I GGTCTC 1 cut(s) 1483
Eco32I GATATC 1 cut(s) 727
Eco47I GGWCC 3 cut(s) 2375, 2538, 2678
Eco53kI GAGCTC 1 cut(s) 368
Eco57I CTGAAG 1 cut(s) 336
Eco88I CYCGRG 3 cut(s) 23, 350, 2900
EcoICRI GAGCTC 1 cut(s) 368
EcoO109I RGGNCCY 2 cut(s) 2538, 2842
EcoRII CCWGG 3 cut(s) 401, 1132, 1598
EcoRV GATATC 1 cut(s) 727
EcoT14I CCWWGG 1 cut(s) 331
EcoT38I GRGCYC 3 cut(s) 370, 2020, 2846
ErhI CCWWGG 1 cut(s) 331
FalI AAGNNNNNCTT 2 cut(s) 2729, 2761
FbaI TGATCA 2 cut(s) 679, 1642
FblI GTMKAC 4 cut(s) 2028, 2510, 2655, 2692
Fnu4HI GCNGC 9 cut(s) 801, 1038, 1959, 2101, 2118, 2331, 2702, 2802, 3033
FokI GGATG 4 cut(s) 908, 1375, 2392, 2687
FriOI GRGCYC 3 cut(s) 370, 2020, 2846
Fsp4HI GCNGC 9 cut(s) 801, 1038, 1959, 2101, 2118, 2331, 2702, 2802, 3033
FspBI CTAG 5 cut(s) 1623, 1869, 2073, 2624, 2952
FspI TGCGCA 1 cut(s) 2104
GlaI GCGC 3 cut(s) 61, 2008, 2104
GluI GCNGC 9 cut(s) 801, 1038, 1959, 2101, 2118, 2331, 2702, 2802, 3033
GsuI CTGGAG 2 cut(s) 1941, 2340
HaeIII GGCC 6 cut(s) 416, 1217, 2615, 2844, 2960, 2978
HapII CCGG 5 cut(s) 194, 611, 770, 2805, 2862
HgaI GACGC 1 cut(s) 2496
HhaI GCGC 3 cut(s) 62, 2009, 2105
Hin6I GCGC 3 cut(s) 60, 2007, 2103
HinP1I GCGC 3 cut(s) 60, 2007, 2103
HincII GTYRAC 2 cut(s) 2511, 2656
HindII GTYRAC 2 cut(s) 2511, 2656
HindIII AAGCTT 2 cut(s) 1817, 2720
HpaII CCGG 5 cut(s) 194, 611, 770, 2805, 2862
Hpy166II GTNNAC 6 cut(s) 781, 2029, 2375, 2511, 2656, 2693
Hpy8I GTNNAC 6 cut(s) 781, 2029, 2375, 2511, 2656, 2693
Hpy99I CGWCG 2 cut(s) 2512, 2657
HpyCH4III ACNGT 5 cut(s) 489, 785, 1205, 2379, 2467
HpyCH4IV ACGT 1 cut(s) 2652
HpySE526I ACGT 1 cut(s) 2652
HspAI GCGC 3 cut(s) 60, 2007, 2103
Ksp22I TGATCA 2 cut(s) 679, 1642
LguI GCTCTTC 1 cut(s) 1763
LmnI GCTCC 6 cut(s) 202, 2023, 2114, 2151, 2525, 2635
Lsp1109I GCAGC 9 cut(s) 787, 1024, 1945, 2087, 2129, 2317, 2688, 2788, 3019
LweI GCATC 6 cut(s) 232, 870, 1355, 1514, 2169, 2953
MaeI CTAG 5 cut(s) 1623, 1869, 2073, 2624, 2952
MaeII ACGT 1 cut(s) 2652
MfeI CAATTG 2 cut(s) 94, 914
MflI RGATCY 6 cut(s) 398, 1027, 1129, 1163, 2001, 2577
MhlI GDGCHC 5 cut(s) 53, 370, 783, 2020, 2846
MlsI TGGCCA 2 cut(s) 1217, 2978
MluNI TGGCCA 2 cut(s) 1217, 2978
MlyI GAGTC 5 cut(s) 670, 728, 1216, 1971, 2086
MmeI TCCRAC 1 cut(s) 868
Mox20I TGGCCA 2 cut(s) 1217, 2978
MroXI GAANNNNTTC 2 cut(s) 1284, 1863
MscI TGGCCA 2 cut(s) 1217, 2978
MslI CAYNNNNRTG 2 cut(s) 1734, 1740
Msp20I TGGCCA 2 cut(s) 1217, 2978
MspA1I CMGCKG 2 cut(s) 1037, 2987
MspI CCGG 5 cut(s) 194, 611, 770, 2805, 2862
MspR9I CCNGG 5 cut(s) 194, 403, 771, 1134, 1600
MunI CAATTG 2 cut(s) 94, 914
Mva1269I GAATGC 4 cut(s) 280, 412, 716, 1375
MvaI CCWGG 3 cut(s) 403, 1134, 1600
NciI CCSGG 2 cut(s) 194, 771
NcoI CCATGG 1 cut(s) 331
NmuCI GTSAC 5 cut(s) 265, 618, 1150, 1931, 2161
NsbI TGCGCA 1 cut(s) 2104
NspI RCATGY 5 cut(s) 1194, 1739, 1994, 2501, 2532
NspV TTCGAA 1 cut(s) 3063
PaeI GCATGC 1 cut(s) 2532
PaeR7I CTCGAG 2 cut(s) 23, 2900
PciI ACATGT 1 cut(s) 2497
PciSI GCTCTTC 1 cut(s) 1763
PcsI WCGNNNNNNNCGW 1 cut(s) 2907
PctI GAATGC 4 cut(s) 280, 412, 716, 1375
PdmI GAANNNNTTC 2 cut(s) 1284, 1863
PfeI GAWTC 9 cut(s) 1064, 1093, 1595, 1907, 2264, 2483, 2667, 2733, 2815
PflMI CCANNNNNTGG 1 cut(s) 1040
PfoI TCCNGGA 3 cut(s) 401, 1132, 1598
PinAI ACCGGT 1 cut(s) 610
PkrI GCNGC 9 cut(s) 802, 1039, 1960, 2102, 2119, 2332, 2703, 2803, 3034
PleI GAGTC 5 cut(s) 669, 728, 1216, 1971, 2085
PpsI GAGTC 5 cut(s) 669, 728, 1216, 1971, 2085
PpuMI RGGWCCY 1 cut(s) 2538
PscI ACATGT 1 cut(s) 2497
PshBI ATTAAT 5 cut(s) 255, 1146, 1320, 2052, 2598
Psp124BI GAGCTC 1 cut(s) 370
Psp5II RGGWCCY 1 cut(s) 2538
Psp6I CCWGG 3 cut(s) 401, 1132, 1598
PspGI CCWGG 3 cut(s) 401, 1132, 1598
PspOMI GGGCCC 1 cut(s) 2842
PspPI GGNCC 5 cut(s) 2375, 2538, 2678, 2842, 2843
PspPPI RGGWCCY 1 cut(s) 2538
PspXI VCTCGAGB 1 cut(s) 2900
PstI CTGCAG 2 cut(s) 805, 2706
PstNI CAGNNNCTG 2 cut(s) 1040, 2004
PsuI RGATCY 6 cut(s) 398, 1027, 1129, 1163, 2001, 2577
PvuII CAGCTG 1 cut(s) 1037
RsaI GTAC 4 cut(s) 134, 787, 1003, 2938
RsaNI GTAC 4 cut(s) 133, 786, 1002, 2937
RseI CAYNNNNRTG 2 cut(s) 1734, 1740
SacI GAGCTC 1 cut(s) 370
SalI GTCGAC 2 cut(s) 2509, 2654
SapI GCTCTTC 1 cut(s) 1763
SatI GCNGC 9 cut(s) 801, 1038, 1959, 2101, 2118, 2331, 2702, 2802, 3033
Sau96I GGNCC 5 cut(s) 2375, 2538, 2678, 2842, 2843
ScaI AGTACT 1 cut(s) 1003
SchI GAGTC 5 cut(s) 670, 728, 1216, 1971, 2086
ScrFI CCNGG 5 cut(s) 194, 403, 771, 1134, 1600
SduI GDGCHC 5 cut(s) 53, 370, 783, 2020, 2846
SfaNI GCATC 6 cut(s) 232, 870, 1355, 1514, 2169, 2953
SfcI CTRYAG 4 cut(s) 801, 1569, 2702, 2988
Sfr274I CTCGAG 2 cut(s) 23, 2900
SfuI TTCGAA 1 cut(s) 3063
SinI GGWCC 3 cut(s) 2375, 2538, 2678
SlaI CTCGAG 2 cut(s) 23, 2900
SmiMI CAYNNNNRTG 2 cut(s) 1734, 1740
SmlI CTYRAG 6 cut(s) 23, 44, 1499, 1979, 2268, 2900
SmoI CTYRAG 6 cut(s) 23, 44, 1499, 1979, 2268, 2900
SpeI ACTAGT 1 cut(s) 2623
SphI GCATGC 1 cut(s) 2532
SsiI CCGC 5 cut(s) 14, 1377, 1447, 1961, 2985
SspMI CTAG 5 cut(s) 1623, 1869, 2073, 2624, 2952
SstI GAGCTC 1 cut(s) 370
StyD4I CCNGG 5 cut(s) 192, 401, 769, 1132, 1598
StyI CCWWGG 1 cut(s) 331
TaaI ACNGT 5 cut(s) 489, 785, 1205, 2379, 2467
TaiI ACGT 1 cut(s) 2655
TatI WGTACW 1 cut(s) 1001
TfiI GAWTC 9 cut(s) 1064, 1093, 1595, 1907, 2264, 2483, 2667, 2733, 2815
TscAI CASTG 5 cut(s) 494, 727, 1458, 1815, 3006
TseFI GTSAC 5 cut(s) 265, 618, 1150, 1931, 2161
TseI GCWGC 9 cut(s) 800, 1037, 1958, 2100, 2117, 2330, 2701, 2801, 3032
Tsp45I GTSAC 5 cut(s) 265, 618, 1150, 1931, 2161
TspGWI ACGGA 1 cut(s) 315
TspRI CASTG 5 cut(s) 494, 727, 1458, 1815, 3006
Van91I CCANNNNNTGG 1 cut(s) 1040
VneI GTGCAC 1 cut(s) 779
VpaK11BI GGWCC 3 cut(s) 2375, 2538, 2678
VspI ATTAAT 5 cut(s) 255, 1146, 1320, 2052, 2598
XapI RAATTY 2 cut(s) 111, 1616
XceI RCATGY 5 cut(s) 1194, 1739, 1994, 2501, 2532
XhoI CTCGAG 2 cut(s) 23, 2900
XmiI GTMKAC 4 cut(s) 2028, 2510, 2655, 2692
XmnI GAANNNNTTC 2 cut(s) 1284, 1863
XspI CTAG 5 cut(s) 1623, 1869, 2073, 2624, 2952
ZrmI AGTACT 1 cut(s) 1003
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.