Rroxscaffold_1G00061440

Protein BREAST CANCER SUSCEPTIBILITY 1 homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0008716
Physical Location & Seq
Reverse (-)
83568871 .. 83574768
5898 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00061440.1

Sequence Viewer

Length: 3270 bp
ATGGGCAATCTGGCGCACCTCGAGAAAATGGGCAGAGAACTCAAATGCCCAATATGTTTGAGTCTGTTTACTTCAGCCGTTTCGCTGAATTGCAACCATGTCTTCTGCAACTCTTGTATTGTGAAATCAATGAAATCGGGTTCCAATTGCCCTGTTTGTAAGATCCCATATCAGCCGAGAGAGGTTCGCCCTGCTCCTCATATGGACAATTTGGTTAGTATTTACAAAAGCATGGAGGATGCTTCGGGTATTAACATGTTTATGACTCAAAGTGCGCTGTCAGCTAAATCATCAGATGGAAAGCAGCAAGCTGATGATGAGAACCCTGATGATCAAGATCCTCCTAAACTTTCTCAGAATAGAGCTGGGAACCGGAGAACTGTAAGAGGGAAAAGATCATGGAAAACTAAATCCAATCTAAAGAACTCTGAATCTGTTTCGGTTAAGCCTTCATTTCCAACCAAGAAAAGGGTTCAGGTTCCACAATGTCCTCCTTCAGAAACCCCTACACGGCCTAAACAGTTACCGGGTGGTTTTATTGATGAAAAGTCGAAGCAGGAGTCTACAACACTAAAGGAGAAGCCTGTTTTAAATGAAAAGGGAGAACCGGTGCTTTCACCTTTCTTTTGGTTGAGTGAAAAAGATGCTGAAAATTTGAGTCAGCCTTCTAGTGGTGATCAGCTTAATGATATTCCATCTCCCAATGTTCCTACTTTCAGTGATATCAAGGACTCAGATGATGAAGACTGTTCTAGATTGAGCCCACTGGGCAAAGTGCAGGGGAAATCTTCCAATGTTGCTGATTTTTTTGATAGTGAAATGTTCGAATGGACGCAAAGGCCGTGCTCCCCTGAGCTCTTTGCAAGTCCTTCTAAGATGCAGGTTGTGGATAGTGATCAAATTGATAGAATTCAAGTGAAGGAACTAAAAGAAGCTTTACTAAACAAGAAAATAGGTGAACAAGTCACTGCTGAAAAAGAAAGGTCCAGAAATTCTAGACAGAGTAGTGGAAACTTAATCTTGCTGCCTGATCTTCCACATGATAAAACTGAAGATGCTAATCATCAATTTCTTTGCAAACCATCTAACAAGAGAGGCAGACAAGCAAGAAAAATAGCTAAAAGAAAGTGTGCTACAAGTCATATGGACCTGGTTGAGAATAGTAGTGCTAATTTGAAGGGTTCTGAAGATTTTTATCAAGAACACGGCTGCAAGAATAGTGACAGTTCTATGGCCAAGGCTGGCAAAAGGAATAAGAAAGCTCATCCGTCTCGGCTGGCAACTAAGCCAACATGTGAAAGTGTTTGCGTCCTCTCTATTGGTGCTGAAAGGCAGAATGAAGGCAGTGCTGAGAAGGGGTTAACTGAATTACCTACTTCACTAGAAAAGAATGAAGGCAGTGATGAAGCTTTTGCCCAGGAGAAGGCTAAGAAAATATGTACGGAGATAAATGCCAAGAACCAGATGTGTTGCTCTGTAAGGTCAAGGAAACAAAAGGTGGTCTCTATGCCCAACATACTCGATGAGGTATCGGAAATCAAGAAGCAAGCAAATAAGAATACTACAACTGAGTCGTCCCATGTAACTGTCCCTACAGATGATAACAGGAAAGTCTCTGACGTCAAGAATATATCCACAAAACTTGCTAGCGAAGCCAAGTCATCTGATCGAGAATTGAAATTCAACAAAAGGGTCAGAGTTTCCTCTGGTGATGACTCAAAGGATAAGGAGGTATCAGAGATCAAGAAGCAAGCAAATAAGAATGCTCCAACTGAGTTGCCCCTTATAAATCTCCCTACAAATGTTAGTAGGGAACTATCAGGAGTCAGAAAACGTGCTAGAGAAGCCAAGTCATGTGATCGAAAAACTACCAAAAAGTTGAGAGTTTCCTTTGATGACAGTTCAAAAGATGAGGCTGTTATTGAGATTCGAGAGGGTCAACATAATGTTAATGAAAATGGTAACCCACCCTCTGAGAAGAGCAAAGGCAATTCCAATGCTAGAATTTTTACTGACCGATCATCAAGGCTTCCTACATTGAAGAATGATGTGGTTTTGAGGAGATGTGATGCCGTTACTAGTAACATTCAATGTGCCTTTTGTCTTTCATCAGAAGAATCAGAGGCATCAGGAGAAATAGTTCACTATTATAATGGCAAGCCTGTTGCTGCAGATCACAATGGAGGGTCCAAGGTTATACATTCACACAGGAACTGCACTGAATGGGCTCCCAACGTATATTTTGAAGATGATATTGCTATGAATCTTGAAGCTGAGCTAACTAGAAGTCGTAGAATTAAATGTTGTTGCTGTGGAATTAAAGGAGCATCTCTTGGGTGTTATGAGAAGAGTTGTCGTAAGAGCTTTCATGTTAGCTGTGCAAAGATGACTCCTGAATGTCGATGGGATTCTGATAATTTTGTCATGTTATGCCCCATTCATGCCTCTTCTAAGTTGCCAAATGAAAGTTCTGAATCTGAAGCAAGGAGGAAGAAGAGCAACCCTAGAAAACAACCTGGTGCTGATCATAATAAAGTTGCTGTTAAACAAGACAGCAATACCTGTCAGGATCTGAAATTTTGTGGATCATCCAAGAAATTGGTTCTCTGTTGTTCAACTCTCACAAATGCAGAGCGGGAATATGTTGCGGAATTTGAAAAATTATCTGGAGTTACAGTCTTAAAGAAGTGGGACTCTAGTATCACACATATTGTTGCGCCAATAGATGAAAATGGAGCATGCAGAAGAACCCTTAAAGTATTGTTGGGAATCTTGGAGGGGAAGTGGATACTGAGCATGGGATGGATTAAAGCTTGCATGGAAGCCATGAAACTAGTGAATGAGGAGCCTTATGAAATTGGCGTTGACATTTATGGAATCAGGGATGGCCCTCGACTTGGTAGACTAAGACTGCAGAACAAGGAACCAAAGCTTTTTGATGGGCTTAAGTTTTACTTCATGGGAGATTTTGTACCTTCATACAAGGGGTATCTACAAGACCTTGTAATAGCTGCTGGAGGAACTGTTTTGCACAGAAAGCCTGTTCCAGAGAGTCAGAAGGGGTCGTCTGGAAGTCCCCTCGAATGTCGAACCTTCATTATCTTTAGTCTTGAGCTACCTGATCAATGTCATCCTAGCAAGAAGGGTACCATTTTCAACCAAAGGCAGGCGGATGCAAAGTCTTTGGCAAGTTCTGCTGGAGCCAATGTAGTAAGCAATTCATGGATTTTGAACTCTGTTGCAGCCTGCAAGTTACAAAATCTCTCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000151 GO:0000152 GO:0000724 GO:0000725 GO:0003674 GO:0003824 GO:0004842 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005886 GO:0006139 GO:0006259 GO:0006281 GO:0006302 GO:0006310 GO:0006355 GO:0006357 GO:0006464 GO:0006725 GO:0006807 GO:0006950 GO:0006974 GO:0008150 GO:0008152 GO:0009314 GO:0009628 GO:0009889 GO:0009890 GO:0009891 GO:0009892 GO:0009893 GO:0009987 GO:0010212 GO:0010332 GO:0010468 GO:0010556 GO:0010557 GO:0010564 GO:0010565 GO:0010604 GO:0010605 GO:0010628 GO:0010638 GO:0010639 GO:0016020 GO:0016567 GO:0016740 GO:0019216 GO:0019217 GO:0019219 GO:0019222 GO:0019538 GO:0019787 GO:0031056 GO:0031057 GO:0031058 GO:0031323 GO:0031324 GO:0031325 GO:0031326 GO:0031327 GO:0031328 GO:0031399 GO:0031400 GO:0031401 GO:0031436 GO:0032268 GO:0032269 GO:0032270 GO:0032446 GO:0032991 GO:0033043 GO:0033044 GO:0033554 GO:0034641 GO:0035065 GO:0035066 GO:0035067 GO:0036211 GO:0042127 GO:0042304 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044464 GO:0045717 GO:0045787 GO:0045833 GO:0045893 GO:0045922 GO:0045935 GO:0045944 GO:0046483 GO:0046890 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0050789 GO:0050794 GO:0050896 GO:0051055 GO:0051128 GO:0051129 GO:0051130 GO:0051171 GO:0051172 GO:0051173 GO:0051246 GO:0051247 GO:0051248 GO:0051252 GO:0051254 GO:0051716 GO:0051726 GO:0060255 GO:0062012 GO:0062014 GO:0065007 GO:0070531 GO:0070647 GO:0071156 GO:0071158 GO:0071214 GO:0071478 GO:0071479 GO:0071480 GO:0071704 GO:0071944 GO:0080090 GO:0090068 GO:0090304 GO:0104004 GO:0140096 GO:1901360 GO:1901564 GO:1901983 GO:1901984 GO:1901985 GO:1902275 GO:1902494 GO:1902680 GO:1903506 GO:1903508 GO:1905268 GO:1905269 GO:1990234 GO:2000112 GO:2000756 GO:2000757 GO:2000758 GO:2001141 GO:2001251 GO:2001252
KEGG Pathways
Metabolic & Signaling
ko03440 map03440
Pfam Domains
Protein Families
BRCT BRCT_2 DYW_deaminase PPR zf-C3HC4_2 zf-HC5HC2H zf-RING_2

Protein Analysis

1089

Amino Acids

120.76

Weight (kDa)

8.92

Isoelectric Point (pI)

52.3

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-RING_6 PF14835 4 - 64 2.8e-06 zf-RING of BARD1-type protein
zf-C3HC4_3 PF13920 13 - 56 1.7e-06 Zinc finger, C3HC4 type (RING finger)
zf-C3HC4_2 PF13923 15 - 53 3.1e-10 Zinc finger, C3HC4 type (RING finger)
zf-RING_2 PF13639 15 - 54 9.1e-08 Ring finger domain
zf-RING_UBOX PF13445 16 - 51 2.2e-07 RING-type zinc-finger
zf-C3HC4 PF00097 16 - 53 7e-08 Zinc finger, C3HC4 type (RING finger)
zf-C3HC4_4 PF15227 16 - 53 1.5e-06 zinc finger of C3HC4-type, RING
zf-HC5HC2H PF13771 736 - 814 3.8e-09 PHD-like zinc-binding domain
BRCT PF00533 866 - 940 1.2e-08 BRCA1 C Terminus (BRCT) domain
BRCT_2 PF16589 977 - 1088 4.5e-08 BRCT domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 1787, 2151
AatII GACGTC 1 cut(s) 1623
Acc36I ACCTGC 1 cut(s) 871
Acc65I GGTACC 1 cut(s) 3146
AccB1I GGYRCC 1 cut(s) 3146
AccBSI CCGCTC 1 cut(s) 2635
AccI GTMKAC 2 cut(s) 563, 2902
AciI CCGC 3 cut(s) 2635, 2648, 3170
AclWI GGATC 4 cut(s) 157, 332, 2577, 2593
AcoI YGGCCR 1 cut(s) 1233
AcsI RAATTY 7 cut(s) 652, 909, 991, 1679, 2004, 2576, 2651
AcuI CTGAAG 5 cut(s) 57, 480, 1071, 1206, 2499
AcyI GRCGYC 1 cut(s) 1620
AfaI GTAC 3 cut(s) 1441, 2973, 3148
AfiI CCNNNNNNNGG 6 cut(s) 468, 510, 671, 1423, 2897, 3166
AflII CTTAAG 1 cut(s) 2945
AflIII ACRYGT 2 cut(s) 255, 1292
AgeI ACCGGT 1 cut(s) 607
AhlI ACTAGT 2 cut(s) 2078, 2833
AjnI CCWGG 3 cut(s) 1149, 1416, 2515
AjuI GAANNNNNNNTTGG 2 cut(s) 2452, 2484
AloI GAACNNNNNNTCC 2 cut(s) 2124, 2156
Alw21I GWGCWC 2 cut(s) 848, 858
Alw26I GTCTC 3 cut(s) 1275, 1507, 1618
AlwI GGATC 4 cut(s) 157, 332, 2577, 2593
AlwNI CAGNNNCTG 2 cut(s) 2214, 2572
Ama87I CYCGRG 1 cut(s) 20
AoxI GGCC 4 cut(s) 512, 839, 1233, 2887
ApeKI GCWGC 6 cut(s) 304, 1024, 1209, 2168, 3011, 3242
ApoI RAATTY 7 cut(s) 652, 909, 991, 1679, 2004, 2576, 2651
ArsI GACNNNNNNTTYG 2 cut(s) 545, 577
AsiGI ACCGGT 1 cut(s) 607
Asp700I GAANNNNTTC 1 cut(s) 2139
Asp718I GGTACC 1 cut(s) 3146
AspLEI GCGC 3 cut(s) 16, 277, 2719
AspS9I GGNCC 4 cut(s) 984, 1147, 2187, 2888
AsuC2I CCSGG 1 cut(s) 528
AsuHPI GGTGA 4 cut(s) 609, 686, 968, 1721
AsuII TTCGAA 1 cut(s) 825
AsuNHI GCTAGC 1 cut(s) 1646
AvaI CYCGRG 1 cut(s) 20
AvaII GGWCC 3 cut(s) 984, 1147, 2187
BaeI ACNNNNGTAYC 2 cut(s) 2683, 2716
BalI TGGCCA 1 cut(s) 1235
BanI GGYRCC 1 cut(s) 3146
BanII GRGCYC 3 cut(s) 764, 858, 2230
BbsI GAAGAC 2 cut(s) 94, 750
Bbv12I GWGCWC 2 cut(s) 848, 858
BbvI GCAGC 6 cut(s) 316, 1011, 1196, 2155, 2998, 3254
BccI CCATC 7 cut(s) 290, 703, 1090, 2397, 2796, 2879, 2933
BceAI ACGGC 5 cut(s) 62, 527, 826, 1222, 2057
BciT130I CCWGG 3 cut(s) 1151, 1418, 2517
BciVI GTATCC 1 cut(s) 2781
BclI TGATCA 5 cut(s) 331, 676, 895, 2524, 3121
BcnI CCSGG 1 cut(s) 528
BcoDI GTCTC 3 cut(s) 1275, 1507, 1618
BcuI ACTAGT 2 cut(s) 2078, 2833
BfmI CTRYAG 3 cut(s) 1593, 2169, 2912
BfrI CTTAAG 1 cut(s) 2945
BfuAI ACCTGC 1 cut(s) 871
BfuI GTATCC 1 cut(s) 2781
BglI GCCNNNNNGGC 1 cut(s) 768
BisI GCNGC 6 cut(s) 305, 1025, 1210, 2169, 3012, 3243
BlpI GCTNAGC 1 cut(s) 2274
BlsI GCNGC 6 cut(s) 306, 1026, 1211, 2170, 3013, 3244
Bme1390I CCNGG 4 cut(s) 528, 1151, 1418, 2517
Bme18I GGWCC 3 cut(s) 984, 1147, 2187
BmeT110I CYCGRG 1 cut(s) 20
BmgT120I GGNCC 4 cut(s) 984, 1147, 2187, 2888
BmiI GGNNCC 9 cut(s) 142, 371, 480, 2188, 2229, 2847, 2925, 3148, 3202
BmrFI CCNGG 4 cut(s) 528, 1151, 1418, 2517
BmrI ACTGGG 1 cut(s) 776
BmsI GCATC 8 cut(s) 229, 634, 867, 1045, 2059, 2135, 2336, 3163
BmtI GCTAGC 1 cut(s) 1650
BmuI ACTGGG 1 cut(s) 776
BpiI GAAGAC 2 cut(s) 94, 750
BpmI CTGGAG 3 cut(s) 2688, 3036, 3219
Bpu10I CCTNAGC 1 cut(s) 852
Bpu1102I GCTNAGC 1 cut(s) 2274
Bpu14I TTCGAA 1 cut(s) 825
BpuEI CTTGAG 1 cut(s) 3131
BpuMI CCSGG 1 cut(s) 528
BsaBI GATNNNNATC 4 cut(s) 336, 894, 1059, 1194
BsaHI GRCGYC 1 cut(s) 1620
BsaI GGTCTC 1 cut(s) 1507
BsaJI CCNNGG 3 cut(s) 1236, 1416, 2190
BsaWI WCCGGW 2 cut(s) 372, 607
Bsc4I CCNNNNNNNGG 6 cut(s) 468, 510, 671, 1423, 2897, 3166
Bse118I RCCGGY 1 cut(s) 607
Bse1I ACTGG 1 cut(s) 771
Bse8I GATNNNNATC 4 cut(s) 336, 894, 1059, 1194
BseBI CCWGG 3 cut(s) 1151, 1418, 2517
BseDI CCNNGG 3 cut(s) 1236, 1416, 2190
BseGI GGATG 7 cut(s) 244, 1264, 2588, 2807, 2890, 3130, 3178
BseJI GATNNNNATC 4 cut(s) 336, 894, 1059, 1194
BseLI CCNNNNNNNGG 6 cut(s) 468, 510, 671, 1423, 2897, 3166
BseMII CTCAG 9 cut(s) 368, 747, 843, 1341, 1560, 1764, 1965, 2265, 2783
BseNI ACTGG 1 cut(s) 771
BseRI GAGGAG 3 cut(s) 186, 2074, 2858
BseXI GCAGC 6 cut(s) 316, 1011, 1196, 2155, 2998, 3254
BseYI CCCAGC 1 cut(s) 365
BsgI GTGCAG 2 cut(s) 797, 2200
BshFI GGCC 4 cut(s) 514, 841, 1235, 2889
BshNI GGYRCC 1 cut(s) 3146
BshTI ACCGGT 1 cut(s) 607
BsiHKAI GWGCWC 2 cut(s) 848, 858
BsiHKCI CYCGRG 1 cut(s) 20
BsiSI CCGG 3 cut(s) 373, 527, 608
BslFI GGGAC 4 cut(s) 1561, 1574, 2705, 3060
BslI CCNNNNNNNGG 6 cut(s) 468, 510, 671, 1423, 2897, 3166
BsmAI GTCTC 3 cut(s) 1275, 1507, 1618
BsmBI CGTCTC 1 cut(s) 1275
BsmFI GGGAC 4 cut(s) 1561, 1574, 2705, 3060
BsmI GAATGC 1 cut(s) 1768
BsnI GGCC 4 cut(s) 514, 841, 1235, 2889
Bso31I GGTCTC 1 cut(s) 1507
BsoBI CYCGRG 1 cut(s) 20
Bsp119I TTCGAA 1 cut(s) 825
Bsp1286I GDGCHC 4 cut(s) 764, 848, 858, 2230
Bsp1720I GCTNAGC 1 cut(s) 2274
BspACI CCGC 3 cut(s) 2635, 2648, 3170
BspANI GGCC 4 cut(s) 514, 841, 1235, 2889
BspCNI CTCAG 9 cut(s) 367, 746, 844, 1342, 1561, 1765, 1966, 2266, 2784
BspLI GGNNCC 9 cut(s) 142, 371, 480, 2188, 2229, 2847, 2925, 3148, 3202
BspMAI CTGCAG 2 cut(s) 2173, 2916
BspMI ACCTGC 1 cut(s) 871
BspOI GCTAGC 1 cut(s) 1650
BspPI GGATC 4 cut(s) 157, 332, 2577, 2593
BspQI GCTCTTC 2 cut(s) 1973, 2489
BspT104I TTCGAA 1 cut(s) 825
BspT107I GGYRCC 1 cut(s) 3146
BspTI CTTAAG 1 cut(s) 2945
BspTNI GGTCTC 1 cut(s) 1507
BsrBI CCGCTC 1 cut(s) 2635
BsrFI RCCGGY 1 cut(s) 607
BsrI ACTGG 1 cut(s) 771
BssAI RCCGGY 1 cut(s) 607
BssECI CCNNGG 3 cut(s) 1236, 1416, 2190
BssNI GRCGYC 1 cut(s) 1620
BssT1I CCWWGG 2 cut(s) 1236, 2190
Bst2UI CCWGG 3 cut(s) 1151, 1418, 2517
Bst4CI ACNGT 8 cut(s) 382, 522, 749, 1226, 1588, 1901, 2677, 3025
Bst6I CTCTTC 4 cut(s) 1973, 2342, 2452, 2489
BstACI GRCGYC 1 cut(s) 1620
BstAFI CTTAAG 1 cut(s) 2945
BstAPI GCANNNNNTGC 1 cut(s) 3194
BstBI TTCGAA 1 cut(s) 825
BstEII GGTNACC 1 cut(s) 1961
BstF5I GGATG 7 cut(s) 244, 1264, 2588, 2807, 2890, 3130, 3178
BstHHI GCGC 3 cut(s) 16, 277, 2719
BstMAI GTCTC 3 cut(s) 1275, 1507, 1618
BstMWI GCNNNNNNNGC 6 cut(s) 281, 768, 1652, 1844, 3037, 3194
BstNI CCWGG 3 cut(s) 1151, 1418, 2517
BstNSI RCATGY 3 cut(s) 259, 1296, 2742
BstPI GGTNACC 1 cut(s) 1961
BstSCI CCNGG 4 cut(s) 526, 1149, 1416, 2515
BstSFI CTRYAG 3 cut(s) 1593, 2169, 2912
BstV1I GCAGC 6 cut(s) 316, 1011, 1196, 2155, 2998, 3254
BstV2I GAAGAC 2 cut(s) 94, 750
BstX2I RGATCY 3 cut(s) 162, 337, 2569
BstXI CCANNNNNNTGG 1 cut(s) 2599
BstYI RGATCY 3 cut(s) 162, 337, 2569
BsuI GTATCC 1 cut(s) 2781
BsuRI GGCC 4 cut(s) 514, 841, 1235, 2889
BtsCI GGATG 7 cut(s) 244, 1264, 2588, 2807, 2890, 3130, 3178
BtsI GCAGTG 3 cut(s) 966, 1351, 1405
BtsIMutI CAGTG 6 cut(s) 724, 764, 966, 1351, 1405, 2217
BveI ACCTGC 1 cut(s) 871
CaiI CAGNNNCTG 2 cut(s) 2214, 2572
CfoI GCGC 3 cut(s) 16, 277, 2719
Cfr10I RCCGGY 1 cut(s) 607
Cfr13I GGNCC 4 cut(s) 984, 1147, 2187, 2888
CseI GACGC 2 cut(s) 841, 1297
CsiI ACCWGGT 2 cut(s) 1149, 2515
Csp6I GTAC 3 cut(s) 1440, 2972, 3147
CspAI ACCGGT 1 cut(s) 607
CspCI CAANNNNNGTGG 2 cut(s) 1624, 1659
CviQI GTAC 3 cut(s) 1440, 2972, 3147
DraI TTTAAA 1 cut(s) 591
EaeI YGGCCR 1 cut(s) 1233
Eam1104I CTCTTC 4 cut(s) 1973, 2342, 2452, 2489
EarI CTCTTC 4 cut(s) 1973, 2342, 2452, 2489
EciI GGCGGA 1 cut(s) 3185
Ecl136II GAGCTC 1 cut(s) 856
Eco130I CCWWGG 2 cut(s) 1236, 2190
Eco24I GRGCYC 3 cut(s) 764, 858, 2230
Eco31I GGTCTC 1 cut(s) 1507
Eco32I GATATC 1 cut(s) 724
Eco47I GGWCC 3 cut(s) 984, 1147, 2187
Eco53kI GAGCTC 1 cut(s) 856
Eco57I CTGAAG 5 cut(s) 57, 480, 1071, 1206, 2499
Eco88I CYCGRG 1 cut(s) 20
Eco91I GGTNACC 1 cut(s) 1961
EcoICRI GAGCTC 1 cut(s) 856
EcoO65I GGTNACC 1 cut(s) 1961
EcoRI GAATTC 1 cut(s) 909
EcoRII CCWGG 3 cut(s) 1149, 1416, 2515
EcoRV GATATC 1 cut(s) 724
EcoT14I CCWWGG 2 cut(s) 1236, 2190
EcoT38I GRGCYC 3 cut(s) 764, 858, 2230
ErhI CCWWGG 2 cut(s) 1236, 2190
Esp3I CGTCTC 1 cut(s) 1275
FalI AAGNNNNNCTT 2 cut(s) 2939, 2971
FaqI GGGAC 4 cut(s) 1561, 1574, 2705, 3060
FauI CCCGC 1 cut(s) 2628
FauNDI CATATG 2 cut(s) 201, 1143
FbaI TGATCA 5 cut(s) 331, 676, 895, 2524, 3121
FblI GTMKAC 2 cut(s) 563, 2902
Fnu4HI GCNGC 6 cut(s) 305, 1025, 1210, 2169, 3012, 3243
FokI GGATG 7 cut(s) 251, 1251, 2575, 2814, 2897, 3117, 3185
FriOI GRGCYC 3 cut(s) 764, 858, 2230
Fsp4HI GCNGC 6 cut(s) 305, 1025, 1210, 2169, 3012, 3243
GlaI GCGC 3 cut(s) 15, 276, 2718
GluI GCNGC 6 cut(s) 305, 1025, 1210, 2169, 3012, 3243
GsaI CCCAGC 1 cut(s) 369
GsuI CTGGAG 3 cut(s) 2688, 3036, 3219
HaeIII GGCC 4 cut(s) 514, 841, 1235, 2889
HapII CCGG 3 cut(s) 373, 527, 608
HgaI GACGC 2 cut(s) 841, 1297
HhaI GCGC 3 cut(s) 16, 277, 2719
Hin1I GRCGYC 1 cut(s) 1620
Hin6I GCGC 3 cut(s) 14, 275, 2717
HinP1I GCGC 3 cut(s) 14, 275, 2717
HincII GTYRAC 3 cut(s) 1362, 1940, 2866
HindII GTYRAC 3 cut(s) 1362, 1940, 2866
HindIII AAGCTT 4 cut(s) 933, 1407, 2811, 2930
HpaI GTTAAC 1 cut(s) 1362
HpaII CCGG 3 cut(s) 373, 527, 608
HphI GGTGA 4 cut(s) 609, 686, 968, 1721
Hpy166II GTNNAC 8 cut(s) 69, 564, 959, 1362, 1940, 2143, 2866, 2903
Hpy8I GTNNAC 8 cut(s) 69, 564, 959, 1362, 1940, 2143, 2866, 2903
HpyCH4III ACNGT 8 cut(s) 382, 522, 749, 1226, 1588, 1901, 2677, 3025
HpyCH4IV ACGT 3 cut(s) 1620, 1834, 2235
HpyF10VI GCNNNNNNNGC 6 cut(s) 281, 768, 1652, 1844, 3037, 3194
HpySE526I ACGT 3 cut(s) 1620, 1834, 2235
Hsp92I GRCGYC 1 cut(s) 1620
HspAI GCGC 3 cut(s) 14, 275, 2717
KpnI GGTACC 1 cut(s) 3150
Ksp22I TGATCA 5 cut(s) 331, 676, 895, 2524, 3121
KspAI GTTAAC 1 cut(s) 1362
LguI GCTCTTC 2 cut(s) 1973, 2489
LmnI GCTCC 8 cut(s) 199, 851, 1771, 2233, 2324, 2735, 2845, 3200
Lsp1109I GCAGC 6 cut(s) 316, 1011, 1196, 2155, 2998, 3254
LweI GCATC 8 cut(s) 229, 634, 867, 1045, 2059, 2135, 2336, 3163
MabI ACCWGGT 2 cut(s) 1149, 2515
MaeII ACGT 3 cut(s) 1620, 1834, 2235
MaeIII GTNAC 9 cut(s) 522, 964, 1220, 1582, 1961, 2074, 2081, 2671, 3252
MbiI CCGCTC 1 cut(s) 2635
MfeI CAATTG 1 cut(s) 145
MflI RGATCY 3 cut(s) 162, 337, 2569
MhlI GDGCHC 4 cut(s) 764, 848, 858, 2230
MlsI TGGCCA 1 cut(s) 1235
MluNI TGGCCA 1 cut(s) 1235
MmeI TCCRAC 2 cut(s) 482, 1793
Mox20I TGGCCA 1 cut(s) 1235
MroXI GAANNNNTTC 1 cut(s) 2139
MscI TGGCCA 1 cut(s) 1235
MslI CAYNNNNRTG 1 cut(s) 260
Msp20I TGGCCA 1 cut(s) 1235
MspCI CTTAAG 1 cut(s) 2945
MspI CCGG 3 cut(s) 373, 527, 608
MspR9I CCNGG 4 cut(s) 528, 1151, 1418, 2517
MunI CAATTG 1 cut(s) 145
Mva1269I GAATGC 1 cut(s) 1768
MvaI CCWGG 3 cut(s) 1151, 1418, 2517
MwoI GCNNNNNNNGC 6 cut(s) 281, 768, 1652, 1844, 3037, 3194
NciI CCSGG 1 cut(s) 528
NdeI CATATG 2 cut(s) 201, 1143
NheI GCTAGC 1 cut(s) 1646
NlaIV GGNNCC 9 cut(s) 142, 371, 480, 2188, 2229, 2847, 2925, 3148, 3202
NmeAIII GCCGAG 2 cut(s) 201, 1252
NmuCI GTSAC 2 cut(s) 964, 1220
NspI RCATGY 3 cut(s) 259, 1296, 2742
NspV TTCGAA 1 cut(s) 825
PaeI GCATGC 1 cut(s) 2742
PaeR7I CTCGAG 1 cut(s) 20
PciI ACATGT 2 cut(s) 255, 1292
PciSI GCTCTTC 2 cut(s) 1973, 2489
PcsI WCGNNNNNNNCGW 1 cut(s) 839
PctI GAATGC 1 cut(s) 1768
PdmI GAANNNNTTC 1 cut(s) 2139
PfeI GAWTC 8 cut(s) 431, 1927, 2117, 2263, 2408, 2474, 2769, 2877
PinAI ACCGGT 1 cut(s) 607
PkrI GCNGC 6 cut(s) 306, 1026, 1211, 2170, 3013, 3244
PscI ACATGT 2 cut(s) 255, 1292
PsiI TTATAA 2 cut(s) 1787, 2151
Psp124BI GAGCTC 1 cut(s) 858
Psp6I CCWGG 3 cut(s) 1149, 1416, 2515
PspEI GGTNACC 1 cut(s) 1961
PspFI CCCAGC 1 cut(s) 365
PspGI CCWGG 3 cut(s) 1149, 1416, 2515
PspN4I GGNNCC 9 cut(s) 142, 371, 480, 2188, 2229, 2847, 2925, 3148, 3202
PspPI GGNCC 4 cut(s) 984, 1147, 2187, 2888
PstI CTGCAG 2 cut(s) 2173, 2916
PstNI CAGNNNCTG 2 cut(s) 2214, 2572
PsuI RGATCY 3 cut(s) 162, 337, 2569
RsaI GTAC 3 cut(s) 1441, 2973, 3148
RsaNI GTAC 3 cut(s) 1440, 2972, 3147
RseI CAYNNNNRTG 1 cut(s) 260
SacI GAGCTC 1 cut(s) 858
SapI GCTCTTC 2 cut(s) 1973, 2489
SatI GCNGC 6 cut(s) 305, 1025, 1210, 2169, 3012, 3243
Sau96I GGNCC 4 cut(s) 984, 1147, 2187, 2888
ScrFI CCNGG 4 cut(s) 528, 1151, 1418, 2517
SduI GDGCHC 4 cut(s) 764, 848, 858, 2230
SexAI ACCWGGT 2 cut(s) 1149, 2515
SfaNI GCATC 8 cut(s) 229, 634, 867, 1045, 2059, 2135, 2336, 3163
SfcI CTRYAG 3 cut(s) 1593, 2169, 2912
Sfr274I CTCGAG 1 cut(s) 20
SfuI TTCGAA 1 cut(s) 825
SinI GGWCC 3 cut(s) 984, 1147, 2187
SlaI CTCGAG 1 cut(s) 20
SmiMI CAYNNNNRTG 1 cut(s) 260
SmlI CTYRAG 3 cut(s) 20, 2945, 3110
SmoI CTYRAG 3 cut(s) 20, 2945, 3110
SpeI ACTAGT 2 cut(s) 2078, 2833
SphI GCATGC 1 cut(s) 2742
SsiI CCGC 3 cut(s) 2635, 2648, 3170
SstI GAGCTC 1 cut(s) 858
StyD4I CCNGG 4 cut(s) 526, 1149, 1416, 2515
StyI CCWWGG 2 cut(s) 1236, 2190
TaaI ACNGT 8 cut(s) 382, 522, 749, 1226, 1588, 1901, 2677, 3025
TaiI ACGT 3 cut(s) 1623, 1837, 2238
TaqII GACCGA 1 cut(s) 2031
TfiI GAWTC 8 cut(s) 431, 1927, 2117, 2263, 2408, 2474, 2769, 2877
TscAI CASTG 6 cut(s) 724, 771, 973, 1351, 1405, 2224
TseFI GTSAC 2 cut(s) 964, 1220
TseI GCWGC 6 cut(s) 304, 1024, 1209, 2168, 3011, 3242
Tsp45I GTSAC 2 cut(s) 964, 1220
TspGWI ACGGA 2 cut(s) 1257, 1457
TspRI CASTG 6 cut(s) 724, 771, 973, 1351, 1405, 2224
Vha464I CTTAAG 1 cut(s) 2945
VpaK11BI GGWCC 3 cut(s) 984, 1147, 2187
XapI RAATTY 7 cut(s) 652, 909, 991, 1679, 2004, 2576, 2651
XbaI TCTAGA 2 cut(s) 752, 995
XceI RCATGY 3 cut(s) 259, 1296, 2742
XhoI CTCGAG 1 cut(s) 20
XmiI GTMKAC 2 cut(s) 563, 2902
XmnI GAANNNNTTC 1 cut(s) 2139
ZraI GACGTC 1 cut(s) 1621
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.