pycom10g21190

Protein BREAST CANCER SUSCEPTIBILITY 1 homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr10
OG0008716
Physical Location & Seq
Reverse (-)
23899854 .. 23904572
4719 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom10g21190.5

Sequence Viewer

Length: 3192 bp
ATGAAATCGGGTTCCAATTGTCCTGTTTGTAAAATCCCATATCGGCGTAGAGGTATGAATCATGATGCTTTTATTTTTGTGCCGAAGAGTTTGAAGGTTCGCCCGGCTCCTCACATGGATAATTTGGTTAGTATTTACAAGAGCATGGAAGATGATTTGGGAATTAATATATTTGTCACTCAGAATGCACCCCCAACTAAATCATCAGGTATTATTTATGTACATGCATTTGAGAATGTTACGTTGCAGTATAAAATTTATGAGTACTATTTAAAACAACAGATATGCAATAGTTTCATTTGCAATTTGCATACAGGTTTAGTGTGGTTTTTAGCTTTTTGGTTTTATATATACTATAGTTTGACATGTGCACTTTTTTTGTTTCCATTTGATTTGCTTTTATTTATTACTGACGGAAAACATCACGCTGAAGATGATTTTAGCGACGGCGAGAAAGATCTTCCTGAGTTTCAGGACAGAGCTCAGAAACAGAAAACCTTAAGAGGAAAAAGATCCAGGAAAGCTAAGGCCAACCTGGAAACTTCTGGTTCTATTTCAATTAAACCTTCATTTCCAAACAAGAAAAGGGTCCAGGTTCCACAGTCTCCTCTCTCAGAAACCCCAACCTGCTATGAAAAGTTAGAAGGTGATTTAATGGCTGATCGAGCGAAGCAGAGTTCAAAAACACTGAAGGAGAAGCCTGTTCTAAATGAGAAGGGAGAACTGGTTCTTTCACCTTTCTTTTGGTTGAATGACAAAGATGTAGAAAATTTGAGTCAGCATTCTTCCGGTGATCAACTTAGTGATCTTCCATCTCCAAATGTTCCCACATTCAGTGATATCAAAGACTCTGATGATGAAAATTCTGTTAGGTCCCCACCGGGAGAAGTGCACGACACATCTTCTAATGCTGCAGATCTATATGATAGTGAGATGTTTGAATGGACACAAAGAGCTTGCTCTCCTGAACTCTTCCCAAGTCCTTCTGAGATGCAGCTTGCAGATGATGAACTCGATAGAGTTCAAGAGAAGGAACCAAAAGAAGTCTCACAAAACAAGAAATCAGATGAACTTTCTGCTAAAAGTGCAAGGTCTAAAAGAAACAAAAAAAGTAGTGGAAATATGGATTTTATGCCAGAGCTTCCCACTGCTGGAACTAAAGATGTTAGTCACGAAGTTGTTAGTAATGAATCTGACAAGCGAGGTAGGAAGGCAAGGAATCTCACGAAAAGAAAATGTGCTACAAGCCATACAGATCCAGGAACAGATATTAATGTCACTTCAAAGGGATCTAAGGTATTTTATGAAGAACAAGCATGTGAGAATAATGGTGGTTCTTTAGCTAAGACTCACAAAAGGAGTAAGAAGGAACATTCATCTGTTGTAACCAAGCCCACATCTGAAAATGTTCATGTCCTCTCTACCAGAGCTGATAGTCGAATTAATGGTGATGAAAATAGGGTAACTGAATCACCTACTTCAATAGGAAAAAATGCTGGCAGTGATGAACCGTCCAGTAAGAAGGTTGGAAATATCTATGGGGAGGTCAGTGACAAGAACAAGATTGACCGGCCAGTAAGGTCAAAAATACAAAAGATGGTCTCCATGAAAAAGACAATGCTTGAGGATGTATCAGAAATACAGAAGCAAGCAAATAAGGTTACTCCTACTGAGTTGTCTCCTACAAATCTTCCCACAGATGATAACCAGAAAGCCTCTGAATCCAGAAATAAATCCAAAAAACTTGCTAGAAAAGCCAAGTCAGGTGATCAAGAACTGAAAAGTAAGAAAAAGCTTTCAGCTTCTTCTGATGGCAACTCAAAGGATGATACTTCTGTTGAGATTGGAGAAGGTCATGGGGATGTTAACGAAAATGGAAATGAAACCACTGAGAAGAGCAAGGACAATCGTGATGTTATAACTGATCAATCACCAGTGCAGAAGCTTCTTTCATTTACCAATGTGGTTTTGAAGAGATGTGAGGCTGTTCCTAGTAAAATCCAGTGTGCTTTCTGTCTTTCATCAGAAGAATCAGAGGCTTCTGGAGAGATAGTCCACTATTGTAATGGCAAGCCTGTTGCTGCGGATCACGATGGAGGACTCAAGGTTATACATGTACACAGGACCTGCGCTGAATGGGCTCCTAATGTCTATTTTGAAGATGATATGGCTATTAATCTTGAAGCTGAATTAACTAGGAGTCGTAAAATTAAATGTTCTTGCTGCGCAATCAAAGGAGCTGCTCTTGGGTGCAGCAAGAAGAGTTGTCGTAGGAGCTTTCATGTTACCTGTGCAAAGCTGATGCCTCAATGTCGCTGGGATACTGATAACTTTATTATGTTATGCCCCATTCATGCCTCCTCCAAGTTGCCAAATGAAAGCCCTGGATCTCAAGCAAGGAGGAAAAAGGGTAATCCTAGAAAAAAACCTAATATTGAATGTCCTAAAGTTGCTGCTAAACAAGACAGCAATATCCCCCCAGATCAGAAGTTTTGTGGATCATCCAAGAAATTGATTCTTTGTTGTTCATCTCTCACAAATGCAGAGAGGGAGTCTGTCTCTGGATTTGAAAGTTTATCTGGACTTACGGTTTTGAAGAATTGGGATTCTAGTGTTACACATATTATTGCATCGACAGATGAAAATGGAGCATGCAGAAGAACCCTCAAGGTTTTGATGGGTATCTTGGAGGGGAAGTGGATTCTGAGTGTGGAGTGGATTAATGCTTGCACAGAAGCAATGAAACTAGTTGATGAGGAGCCTTATGAAATCAACGTTGACATTTATGGAATCAGGGATGGTCCTCGACTTGGTAGACTAAGGCTGAAGAACAAGCAACCAAAGCTTTTTGAGGGGTTCAAGTTTTACTTCATGGGAAACTTCATACCTTCATACAAGGGGTATTTACAAGACCTTGTAATAGCTGCTGGAGGAACGATTTGGCAAAGAAAACCTGTTCCAGAGGGCCCGAGAGCCTTGTCAGTCGATTCCCCCAAATGTCAAACCTTCATAATTTACAGCCTCGAGCTACCTGACCAATATCATTGCAGCAAGAAGAGTACAATTTTCAACACTAGGCAGGCTGATGCGAAGGCTTTGGCCACCTCCGCTGGAGCCAAGGTAGCAAGCAACTCATGGGTATTGAACTCCATTGCTGCCTGCAAGTTACAAAATCTCTCTGATTCCAAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000151 GO:0000152 GO:0000724 GO:0000725 GO:0003674 GO:0003824 GO:0004842 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005886 GO:0006139 GO:0006259 GO:0006281 GO:0006302 GO:0006310 GO:0006355 GO:0006357 GO:0006464 GO:0006725 GO:0006807 GO:0006950 GO:0006974 GO:0008150 GO:0008152 GO:0009314 GO:0009628 GO:0009889 GO:0009890 GO:0009891 GO:0009892 GO:0009893 GO:0009987 GO:0010212 GO:0010332 GO:0010468 GO:0010556 GO:0010557 GO:0010564 GO:0010565 GO:0010604 GO:0010605 GO:0010628 GO:0010638 GO:0010639 GO:0016020 GO:0016567 GO:0016740 GO:0019216 GO:0019217 GO:0019219 GO:0019222 GO:0019538 GO:0019787 GO:0031056 GO:0031057 GO:0031058 GO:0031323 GO:0031324 GO:0031325 GO:0031326 GO:0031327 GO:0031328 GO:0031399 GO:0031400 GO:0031401 GO:0031436 GO:0032268 GO:0032269 GO:0032270 GO:0032446 GO:0032991 GO:0033043 GO:0033044 GO:0033554 GO:0034641 GO:0035065 GO:0035066 GO:0035067 GO:0036211 GO:0042127 GO:0042304 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044464 GO:0045717 GO:0045787 GO:0045833 GO:0045893 GO:0045922 GO:0045935 GO:0045944 GO:0046483 GO:0046890 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0050789 GO:0050794 GO:0050896 GO:0051055 GO:0051128 GO:0051129 GO:0051130 GO:0051171 GO:0051172 GO:0051173 GO:0051246 GO:0051247 GO:0051248 GO:0051252 GO:0051254 GO:0051716 GO:0051726 GO:0060255 GO:0062012 GO:0062014 GO:0065007 GO:0070531 GO:0070647 GO:0071156 GO:0071158 GO:0071214 GO:0071478 GO:0071479 GO:0071480 GO:0071704 GO:0071944 GO:0080090 GO:0090068 GO:0090304 GO:0104004 GO:0140096 GO:1901360 GO:1901564 GO:1901983 GO:1901984 GO:1901985 GO:1902275 GO:1902494 GO:1902680 GO:1903506 GO:1903508 GO:1905268 GO:1905269 GO:1990234 GO:2000112 GO:2000756 GO:2000757 GO:2000758 GO:2001141 GO:2001251 GO:2001252
KEGG Pathways
Metabolic & Signaling
ko03440 map03440
Pfam Domains
Protein Families
BRCT BRCT_2 DYW_deaminase PPR zf-C3HC4_2 zf-HC5HC2H zf-RING_2

Protein Analysis

1064

Amino Acids

118.36

Weight (kDa)

8.71

Isoelectric Point (pI)

47.26

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1919
Acc16I TGCGCA 1 cut(s) 2227
Acc36I ACCTGC 2 cut(s) 635, 2135
AccI GTMKAC 1 cut(s) 2815
AciI CCGC 2 cut(s) 2084, 3108
AclI AACGTT 1 cut(s) 2775
AclWI GGATC 6 cut(s) 507, 1250, 1297, 2094, 2395, 2506
AcoI YGGCCR 2 cut(s) 1571, 3099
AcsI RAATTY 3 cut(s) 255, 769, 862
AcuI CTGAAG 3 cut(s) 450, 710, 2846
AfaI GTAC 4 cut(s) 222, 266, 2118, 3061
AfiI CCNNNNNNNGG 2 cut(s) 1151, 2810
AflII CTTAAG 1 cut(s) 499
AflIII ACRYGT 2 cut(s) 365, 2113
AhlI ACTAGT 1 cut(s) 2746
AjnI CCWGG 5 cut(s) 515, 534, 591, 1258, 2383
AloI GAACNNNNNNTCC 2 cut(s) 711, 743
Alw21I GWGCWC 3 cut(s) 373, 484, 894
Alw26I GTCTC 5 cut(s) 609, 1051, 1606, 1683, 2563
Alw44I GTGCAC 2 cut(s) 369, 890
AlwI GGATC 6 cut(s) 507, 1250, 1297, 2094, 2395, 2506
AlwNI CAGNNNCTG 1 cut(s) 2127
Ama87I CYCGRG 2 cut(s) 2968, 3023
AoxI GGCC 4 cut(s) 528, 1571, 2965, 3099
ApaI GGGCCC 1 cut(s) 2969
ApaLI GTGCAC 2 cut(s) 369, 890
ApoI RAATTY 3 cut(s) 255, 769, 862
AseI ATTAAT 5 cut(s) 165, 1272, 1443, 2175, 2721
Asp700I GAANNNNTTC 2 cut(s) 726, 1407
AspLEI GCGC 2 cut(s) 2132, 2228
AspS9I GGNCC 6 cut(s) 589, 873, 2124, 2801, 2965, 2966
AsuC2I CCSGG 2 cut(s) 104, 882
AsuHPI GGTGA 7 cut(s) 659, 726, 803, 1460, 1464, 1778, 1923
AvaI CYCGRG 2 cut(s) 2968, 3023
AvaII GGWCC 4 cut(s) 589, 873, 2124, 2801
BaeGI GKGCMC 3 cut(s) 373, 894, 2969
BaeI ACNNNNGTAYC 2 cut(s) 1821, 1854
BalI TGGCCA 1 cut(s) 3101
BanII GRGCYC 3 cut(s) 484, 2143, 2969
Bbv12I GWGCWC 3 cut(s) 373, 484, 894
BccI CCATC 6 cut(s) 820, 1591, 1805, 2087, 2671, 2792
BceAI ACGGC 1 cut(s) 463
BciT130I CCWGG 5 cut(s) 517, 536, 593, 1260, 2385
BciVI GTATCC 1 cut(s) 2314
BclI TGATCA 3 cut(s) 793, 1768, 1924
BcnI CCSGG 2 cut(s) 104, 882
BcoDI GTCTC 5 cut(s) 609, 1051, 1606, 1683, 2563
BcuI ACTAGT 1 cut(s) 2746
BfaI CTAG 7 cut(s) 1749, 1992, 2196, 2418, 2610, 2747, 3075
BfmI CTRYAG 2 cut(s) 355, 912
BfrI CTTAAG 1 cut(s) 499
BfuAI ACCTGC 2 cut(s) 635, 2135
BfuI GTATCC 1 cut(s) 2314
BglII AGATCT 2 cut(s) 457, 916
BmcAI AGTACT 1 cut(s) 266
Bme1390I CCNGG 7 cut(s) 104, 517, 536, 593, 882, 1260, 2385
Bme18I GGWCC 4 cut(s) 589, 873, 2124, 2801
BmeT110I CYCGRG 2 cut(s) 2968, 3023
BmgT120I GGNCC 6 cut(s) 589, 873, 2124, 2801, 2965, 2966
BmrFI CCNGG 7 cut(s) 104, 517, 536, 593, 882, 1260, 2385
BmsI GCATC 5 cut(s) 55, 981, 2292, 2639, 3076
BplI GAGNNNNNCTC 2 cut(s) 2543, 2575
BpmI CTGGAG 3 cut(s) 2064, 2949, 3132
Bpu10I CCTNAGC 1 cut(s) 525
BpuEI CTTGAG 4 cut(s) 1643, 2087, 2376, 2651
BpuMI CCSGG 2 cut(s) 104, 882
BsaBI GATNNNNATC 1 cut(s) 2681
BsaI GGTCTC 1 cut(s) 1606
BsaJI CCNNGG 2 cut(s) 2383, 3117
BsaWI WCCGGW 1 cut(s) 788
BsaXI ACNNNNNCTCC 8 cut(s) 711, 741, 2037, 2067, 2681, 2711, 3105, 3135
Bsc4I CCNNNNNNNGG 2 cut(s) 1151, 2810
Bse118I RCCGGY 1 cut(s) 1569
Bse1I ACTGG 5 cut(s) 729, 1515, 1574, 1934, 2002
Bse3DI GCAATG 3 cut(s) 2745, 3043, 3150
Bse8I GATNNNNATC 1 cut(s) 2681
BseBI CCWGG 5 cut(s) 517, 536, 593, 1260, 2385
BseDI CCNNGG 2 cut(s) 2383, 3117
BseGI GGATG 5 cut(s) 1633, 1831, 1867, 2501, 2803
BseJI GATNNNNATC 1 cut(s) 2681
BseLI CCNNNNNNNGG 2 cut(s) 1151, 2810
BseMI GCAATG 3 cut(s) 2745, 3043, 3150
BseMII CTCAG 8 cut(s) 194, 456, 497, 627, 978, 1662, 1881, 2696
BseNI ACTGG 5 cut(s) 729, 1515, 1574, 1934, 2002
BseRI GAGGAG 4 cut(s) 99, 597, 2350, 2771
BseSI GKGCMC 3 cut(s) 373, 894, 2969
BseYI CCCAGC 1 cut(s) 2316
BsgI GTGCAG 2 cut(s) 1958, 2272
BshFI GGCC 4 cut(s) 530, 1573, 2967, 3101
BsiHKAI GWGCWC 3 cut(s) 373, 484, 894
BsiHKCI CYCGRG 2 cut(s) 2968, 3023
BsiSI CCGG 4 cut(s) 104, 789, 881, 1570
BslFI GGGAC 1 cut(s) 859
BslI CCNNNNNNNGG 2 cut(s) 1151, 2810
BsmAI GTCTC 5 cut(s) 609, 1051, 1606, 1683, 2563
BsmFI GGGAC 1 cut(s) 859
BsmI GAATGC 2 cut(s) 190, 781
BsnI GGCC 4 cut(s) 530, 1573, 2967, 3101
Bso31I GGTCTC 1 cut(s) 1606
BsoBI CYCGRG 2 cut(s) 2968, 3023
Bsp120I GGGCCC 1 cut(s) 2965
Bsp1286I GDGCHC 5 cut(s) 373, 484, 894, 2143, 2969
Bsp1407I TGTACA 2 cut(s) 220, 2116
BspACI CCGC 2 cut(s) 2084, 3108
BspANI GGCC 4 cut(s) 530, 1573, 2967, 3101
BspCNI CTCAG 8 cut(s) 193, 457, 496, 626, 979, 1663, 1882, 2697
BspHI TCATGA 1 cut(s) 61
BspMAI CTGCAG 1 cut(s) 916
BspMI ACCTGC 2 cut(s) 635, 2135
BspPI GGATC 6 cut(s) 507, 1250, 1297, 2094, 2395, 2506
BspQI GCTCTTC 1 cut(s) 1889
BspTI CTTAAG 1 cut(s) 499
BspTNI GGTCTC 1 cut(s) 1606
BsrDI GCAATG 3 cut(s) 2745, 3043, 3150
BsrFI RCCGGY 1 cut(s) 1569
BsrGI TGTACA 2 cut(s) 220, 2116
BsrI ACTGG 5 cut(s) 729, 1515, 1574, 1934, 2002
BssAI RCCGGY 1 cut(s) 1569
BssECI CCNNGG 2 cut(s) 2383, 3117
BssT1I CCWWGG 1 cut(s) 3117
Bst2UI CCWGG 5 cut(s) 517, 536, 593, 1260, 2385
Bst4CI ACNGT 3 cut(s) 603, 1512, 2590
Bst6I CTCTTC 6 cut(s) 80, 977, 1889, 1967, 2255, 3050
BstAFI CTTAAG 1 cut(s) 499
BstAUI TGTACA 2 cut(s) 220, 2116
BstF5I GGATG 5 cut(s) 1633, 1831, 1867, 2501, 2803
BstHHI GCGC 2 cut(s) 2132, 2228
BstMAI GTCTC 5 cut(s) 609, 1051, 1606, 1683, 2563
BstMWI GCNNNNNNNGC 7 cut(s) 665, 1085, 1754, 2138, 2842, 3107, 3122
BstNI CCWGG 5 cut(s) 517, 536, 593, 1260, 2385
BstNSI RCATGY 5 cut(s) 227, 369, 1320, 2117, 2655
BstSCI CCNGG 7 cut(s) 102, 515, 534, 591, 880, 1258, 2383
BstSFI CTRYAG 2 cut(s) 355, 912
BstSLI GKGCMC 3 cut(s) 373, 894, 2969
BstX2I RGATCY 6 cut(s) 457, 512, 916, 1255, 1289, 2387
BstYI RGATCY 6 cut(s) 457, 512, 916, 1255, 1289, 2387
BsuI GTATCC 1 cut(s) 2314
BsuRI GGCC 4 cut(s) 530, 1573, 2967, 3101
BtsCI GGATG 5 cut(s) 1633, 1831, 1867, 2501, 2803
BtsI GCAGTG 2 cut(s) 1146, 1507
BtsIMutI CAGTG 8 cut(s) 686, 841, 1146, 1507, 1555, 1887, 1941, 2009
BveI ACCTGC 2 cut(s) 635, 2135
CaiI CAGNNNCTG 1 cut(s) 2127
CciI TCATGA 1 cut(s) 61
CfoI GCGC 2 cut(s) 2132, 2228
Cfr10I RCCGGY 1 cut(s) 1569
Cfr13I GGNCC 6 cut(s) 589, 873, 2124, 2801, 2965, 2966
Csp6I GTAC 4 cut(s) 221, 265, 2117, 3060
CviQI GTAC 4 cut(s) 221, 265, 2117, 3060
DraI TTTAAA 1 cut(s) 273
EaeI YGGCCR 2 cut(s) 1571, 3099
Eam1104I CTCTTC 6 cut(s) 80, 977, 1889, 1967, 2255, 3050
EarI CTCTTC 6 cut(s) 80, 977, 1889, 1967, 2255, 3050
Ecl136II GAGCTC 1 cut(s) 482
Eco130I CCWWGG 1 cut(s) 3117
Eco24I GRGCYC 3 cut(s) 484, 2143, 2969
Eco31I GGTCTC 1 cut(s) 1606
Eco32I GATATC 1 cut(s) 841
Eco47I GGWCC 4 cut(s) 589, 873, 2124, 2801
Eco53kI GAGCTC 1 cut(s) 482
Eco57I CTGAAG 3 cut(s) 450, 710, 2846
Eco88I CYCGRG 2 cut(s) 2968, 3023
EcoICRI GAGCTC 1 cut(s) 482
EcoO109I RGGNCCY 3 cut(s) 873, 2124, 2965
EcoRII CCWGG 5 cut(s) 515, 534, 591, 1258, 2383
EcoRV GATATC 1 cut(s) 841
EcoT14I CCWWGG 1 cut(s) 3117
EcoT22I ATGCAT 1 cut(s) 229
EcoT38I GRGCYC 3 cut(s) 484, 2143, 2969
ErhI CCWWGG 1 cut(s) 3117
FalI AAGNNNNNCTT 2 cut(s) 2852, 2884
FaqI GGGAC 1 cut(s) 859
FbaI TGATCA 3 cut(s) 793, 1768, 1924
FblI GTMKAC 1 cut(s) 2815
FokI GGATG 5 cut(s) 1640, 1838, 1874, 2488, 2810
FriOI GRGCYC 3 cut(s) 484, 2143, 2969
FspBI CTAG 7 cut(s) 1749, 1992, 2196, 2418, 2610, 2747, 3075
FspI TGCGCA 1 cut(s) 2227
GlaI GCGC 2 cut(s) 2131, 2227
GsaI CCCAGC 1 cut(s) 2320
GsuI CTGGAG 3 cut(s) 2064, 2949, 3132
HaeIII GGCC 4 cut(s) 530, 1573, 2967, 3101
HapII CCGG 4 cut(s) 104, 789, 881, 1570
HhaI GCGC 2 cut(s) 2132, 2228
Hin6I GCGC 2 cut(s) 2130, 2226
HinP1I GCGC 2 cut(s) 2130, 2226
HincII GTYRAC 2 cut(s) 1867, 2779
HindII GTYRAC 2 cut(s) 1867, 2779
HindIII AAGCTT 3 cut(s) 1793, 1943, 2843
HpaI GTTAAC 1 cut(s) 1867
HpaII CCGG 4 cut(s) 104, 789, 881, 1570
HphI GGTGA 7 cut(s) 659, 726, 803, 1460, 1464, 1778, 1923
Hpy166II GTNNAC 7 cut(s) 371, 892, 1867, 2056, 2119, 2779, 2816
Hpy8I GTNNAC 7 cut(s) 371, 892, 1867, 2056, 2119, 2779, 2816
Hpy99I CGWCG 1 cut(s) 449
HpyCH4III ACNGT 3 cut(s) 603, 1512, 2590
HpyCH4IV ACGT 2 cut(s) 242, 2775
HpyF10VI GCNNNNNNNGC 7 cut(s) 665, 1085, 1754, 2138, 2842, 3107, 3122
HpySE526I ACGT 2 cut(s) 242, 2775
HspAI GCGC 2 cut(s) 2130, 2226
Ksp22I TGATCA 3 cut(s) 793, 1768, 1924
KspAI GTTAAC 1 cut(s) 1867
LguI GCTCTTC 1 cut(s) 1889
LmnI GCTCC 7 cut(s) 112, 2146, 2237, 2274, 2648, 2758, 3113
LweI GCATC 5 cut(s) 55, 981, 2292, 2639, 3076
MaeI CTAG 7 cut(s) 1749, 1992, 2196, 2418, 2610, 2747, 3075
MaeII ACGT 2 cut(s) 242, 2775
MfeI CAATTG 1 cut(s) 16
MflI RGATCY 6 cut(s) 457, 512, 916, 1255, 1289, 2387
MhlI GDGCHC 5 cut(s) 373, 484, 894, 2143, 2969
MlsI TGGCCA 1 cut(s) 3101
MluNI TGGCCA 1 cut(s) 3101
MlyI GAGTC 6 cut(s) 784, 842, 1342, 2094, 2209, 2561
MmeI TCCRAC 1 cut(s) 1507
Mox20I TGGCCA 1 cut(s) 3101
Mph1103I ATGCAT 1 cut(s) 229
MroXI GAANNNNTTC 2 cut(s) 726, 1407
MscI TGGCCA 1 cut(s) 3101
MslI CAYNNNNRTG 1 cut(s) 1860
Msp20I TGGCCA 1 cut(s) 3101
MspA1I CMGCKG 1 cut(s) 3110
MspCI CTTAAG 1 cut(s) 499
MspI CCGG 4 cut(s) 104, 789, 881, 1570
MspR9I CCNGG 7 cut(s) 104, 517, 536, 593, 882, 1260, 2385
MunI CAATTG 1 cut(s) 16
Mva1269I GAATGC 2 cut(s) 190, 781
MvaI CCWGG 5 cut(s) 517, 536, 593, 1260, 2385
MwoI GCNNNNNNNGC 7 cut(s) 665, 1085, 1754, 2138, 2842, 3107, 3122
NciI CCSGG 2 cut(s) 104, 882
NmuCI GTSAC 4 cut(s) 175, 1169, 1276, 1550
NsbI TGCGCA 1 cut(s) 2227
NsiI ATGCAT 1 cut(s) 229
NspI RCATGY 5 cut(s) 227, 369, 1320, 2117, 2655
PaeI GCATGC 1 cut(s) 2655
PaeR7I CTCGAG 1 cut(s) 3023
PagI TCATGA 1 cut(s) 61
PciI ACATGT 2 cut(s) 365, 2113
PciSI GCTCTTC 1 cut(s) 1889
PctI GAATGC 2 cut(s) 190, 781
PdmI GAANNNNTTC 2 cut(s) 726, 1407
PfoI TCCNGGA 2 cut(s) 515, 1258
PleI GAGTC 6 cut(s) 783, 842, 1342, 2094, 2208, 2560
PpsI GAGTC 6 cut(s) 783, 842, 1342, 2094, 2208, 2560
PpuMI RGGWCCY 2 cut(s) 873, 2124
PscI ACATGT 2 cut(s) 365, 2113
PshBI ATTAAT 5 cut(s) 165, 1272, 1443, 2175, 2721
PsiI TTATAA 1 cut(s) 1919
Psp124BI GAGCTC 1 cut(s) 484
Psp1406I AACGTT 1 cut(s) 2775
Psp5II RGGWCCY 2 cut(s) 873, 2124
Psp6I CCWGG 5 cut(s) 515, 534, 591, 1258, 2383
PspFI CCCAGC 1 cut(s) 2316
PspGI CCWGG 5 cut(s) 515, 534, 591, 1258, 2383
PspOMI GGGCCC 1 cut(s) 2965
PspPI GGNCC 6 cut(s) 589, 873, 2124, 2801, 2965, 2966
PspPPI RGGWCCY 2 cut(s) 873, 2124
PspXI VCTCGAGB 1 cut(s) 3023
PstI CTGCAG 1 cut(s) 916
PstNI CAGNNNCTG 1 cut(s) 2127
PsuI RGATCY 6 cut(s) 457, 512, 916, 1255, 1289, 2387
RsaI GTAC 4 cut(s) 222, 266, 2118, 3061
RsaNI GTAC 4 cut(s) 221, 265, 2117, 3060
RseI CAYNNNNRTG 1 cut(s) 1860
SacI GAGCTC 1 cut(s) 484
SapI GCTCTTC 1 cut(s) 1889
Sau96I GGNCC 6 cut(s) 589, 873, 2124, 2801, 2965, 2966
ScaI AGTACT 1 cut(s) 266
SchI GAGTC 6 cut(s) 784, 842, 1342, 2094, 2209, 2561
ScrFI CCNGG 7 cut(s) 104, 517, 536, 593, 882, 1260, 2385
SduI GDGCHC 5 cut(s) 373, 484, 894, 2143, 2969
SfaNI GCATC 5 cut(s) 55, 981, 2292, 2639, 3076
SfcI CTRYAG 2 cut(s) 355, 912
Sfr274I CTCGAG 1 cut(s) 3023
SinI GGWCC 4 cut(s) 589, 873, 2124, 2801
SlaI CTCGAG 1 cut(s) 3023
SmiMI CAYNNNNRTG 1 cut(s) 1860
SmlI CTYRAG 6 cut(s) 499, 1622, 2102, 2391, 2666, 3023
SmoI CTYRAG 6 cut(s) 499, 1622, 2102, 2391, 2666, 3023
SpeI ACTAGT 1 cut(s) 2746
SphI GCATGC 1 cut(s) 2655
SsiI CCGC 2 cut(s) 2084, 3108
SspI AATATT 1 cut(s) 2434
SspMI CTAG 7 cut(s) 1749, 1992, 2196, 2418, 2610, 2747, 3075
SstI GAGCTC 1 cut(s) 484
StyD4I CCNGG 7 cut(s) 102, 515, 534, 591, 880, 1258, 2383
StyI CCWWGG 1 cut(s) 3117
TaaI ACNGT 3 cut(s) 603, 1512, 2590
TaiI ACGT 2 cut(s) 245, 2778
TaqI TCGA 7 cut(s) 664, 1014, 1438, 2633, 2806, 2985, 3024
TatI WGTACW 4 cut(s) 220, 264, 2116, 3059
TscAI CASTG 8 cut(s) 693, 841, 1153, 1507, 1555, 1894, 1941, 2009
TseFI GTSAC 4 cut(s) 175, 1169, 1276, 1550
Tsp45I GTSAC 4 cut(s) 175, 1169, 1276, 1550
TspGWI ACGGA 1 cut(s) 429
TspRI CASTG 8 cut(s) 693, 841, 1153, 1507, 1555, 1894, 1941, 2009
Vha464I CTTAAG 1 cut(s) 499
VneI GTGCAC 2 cut(s) 369, 890
VpaK11BI GGWCC 4 cut(s) 589, 873, 2124, 2801
VspI ATTAAT 5 cut(s) 165, 1272, 1443, 2175, 2721
XapI RAATTY 3 cut(s) 255, 769, 862
XceI RCATGY 5 cut(s) 227, 369, 1320, 2117, 2655
XcmI CCANNNNNNNNNTGG 1 cut(s) 2063
XhoI CTCGAG 1 cut(s) 3023
XmiI GTMKAC 1 cut(s) 2815
XmnI GAANNNNTTC 2 cut(s) 726, 1407
XspI CTAG 7 cut(s) 1749, 1992, 2196, 2418, 2610, 2747, 3075
ZrmI AGTACT 1 cut(s) 266
Zsp2I ATGCAT 1 cut(s) 229
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.