MD08G1025000.v1.1

Protein BREAST CANCER SUSCEPTIBILITY 1 homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr08
OG0008716
Physical Location & Seq
Reverse (-)
1796553 .. 1797678
1126 bp
Loading structure...
UTR
Exon/CDS
Intron
MD08G1025000.v1.1.491

Sequence Viewer

Length: 351 bp
ATGAAAATATTCCACAATAAAACCTCTCATTCTCCTCCTTGCGCTGGTGCCTTCTCCCTTCCCCCTTCCTTCCCCCTTTTCTCTTCTCCCCATTTCCTTTTCCTTTGTATTTTTGATTTTGACAAGGAGTCTGTCTCTGGATTTGAAAGATTATTTGGACTTACAGTTTTGAAGAATTGGGATTCAAGTGTTACATATATTATTGCATCGACAGATGAAAATGGAGCATGCAGAAGAACCCTCAAAGTTTTGATGGATATCTTGGAGGGGAAGTGGATTCTAAGTGGCACGGTTGACACACCCCGATCCGGAGGATCCAGGTGTGCTCGCCGTCACGTGGGCGTGACGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000151 GO:0000152 GO:0000724 GO:0000725 GO:0003674 GO:0003824 GO:0004842 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005886 GO:0006139 GO:0006259 GO:0006281 GO:0006302 GO:0006310 GO:0006355 GO:0006357 GO:0006464 GO:0006725 GO:0006807 GO:0006950 GO:0006974 GO:0008150 GO:0008152 GO:0009314 GO:0009628 GO:0009889 GO:0009890 GO:0009891 GO:0009892 GO:0009893 GO:0009987 GO:0010212 GO:0010332 GO:0010468 GO:0010556 GO:0010557 GO:0010564 GO:0010565 GO:0010604 GO:0010605 GO:0010628 GO:0010638 GO:0010639 GO:0016020 GO:0016567 GO:0016740 GO:0019216 GO:0019217 GO:0019219 GO:0019222 GO:0019538 GO:0019787 GO:0031056 GO:0031057 GO:0031058 GO:0031323 GO:0031324 GO:0031325 GO:0031326 GO:0031327 GO:0031328 GO:0031399 GO:0031400 GO:0031401 GO:0031436 GO:0032268 GO:0032269 GO:0032270 GO:0032446 GO:0032991 GO:0033043 GO:0033044 GO:0033554 GO:0034641 GO:0035065 GO:0035066 GO:0035067 GO:0036211 GO:0042127 GO:0042304 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044464 GO:0045717 GO:0045787 GO:0045833 GO:0045893 GO:0045922 GO:0045935 GO:0045944 GO:0046483 GO:0046890 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0050789 GO:0050794 GO:0050896 GO:0051055 GO:0051128 GO:0051129 GO:0051130 GO:0051171 GO:0051172 GO:0051173 GO:0051246 GO:0051247 GO:0051248 GO:0051252 GO:0051254 GO:0051716 GO:0051726 GO:0060255 GO:0062012 GO:0062014 GO:0065007 GO:0070531 GO:0070647 GO:0071156 GO:0071158 GO:0071214 GO:0071478 GO:0071479 GO:0071480 GO:0071704 GO:0071944 GO:0080090 GO:0090068 GO:0090304 GO:0104004 GO:0140096 GO:1901360 GO:1901564 GO:1901983 GO:1901984 GO:1901985 GO:1902275 GO:1902494 GO:1902680 GO:1903506 GO:1903508 GO:1905268 GO:1905269 GO:1990234 GO:2000112 GO:2000756 GO:2000757 GO:2000758 GO:2001141 GO:2001251 GO:2001252
KEGG Pathways
Metabolic & Signaling
ko03440 map03440
Pfam Domains
Protein Families
BRCT BRCT_2 DYW_deaminase PPR zf-C3HC4_2 zf-HC5HC2H zf-RING_2

Protein Analysis

117

Amino Acids

12.85

Weight (kDa)

8.82

Isoelectric Point (pI)

47.39

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
BRCT PF00533 23 - 95 4.1e-06 BRCA1 C Terminus (BRCT) domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 47
AccIII TCCGGA 1 cut(s) 308
AclWI GGATC 3 cut(s) 300, 309, 322
AcvI CACGTG 1 cut(s) 337
AfiI CCNNNNNNNGG 3 cut(s) 44, 308, 337
AgsI TTSAA 3 cut(s) 146, 172, 186
AhdI GACNNNNNGTC 1 cut(s) 127
AjnI CCWGG 1 cut(s) 317
AjuI GAANNNNNNNTTGG 2 cut(s) 138, 170
Alw21I GWGCWC 1 cut(s) 328
Alw26I GTCTC 1 cut(s) 139
AlwI GGATC 3 cut(s) 300, 309, 322
Aor13HI TCCGGA 1 cut(s) 308
Asp700I GAANNNNTTC 1 cut(s) 8
AspLEI GCGC 1 cut(s) 44
BamHI GGATCC 1 cut(s) 314
BanI GGYRCC 1 cut(s) 47
BbrPI CACGTG 1 cut(s) 337
Bbv12I GWGCWC 1 cut(s) 328
BccI CCATC 1 cut(s) 247
BceAI ACGGC 1 cut(s) 315
BciT130I CCWGG 1 cut(s) 319
BcoDI GTCTC 1 cut(s) 139
Bme1390I CCNGG 1 cut(s) 319
BmeRI GACNNNNNGTC 1 cut(s) 127
BmiI GGNNCC 2 cut(s) 49, 316
BmrFI CCNGG 1 cut(s) 319
BmsI GCATC 1 cut(s) 215
BplI GAGNNNNNCTC 2 cut(s) 119, 151
BsaAI YACGTR 1 cut(s) 337
BsaBI GATNNNNATC 1 cut(s) 257
BsaWI WCCGGW 1 cut(s) 308
BsaXI ACNNNNNCTCC 2 cut(s) 257, 287
Bsc4I CCNNNNNNNGG 3 cut(s) 44, 308, 337
Bse8I GATNNNNATC 1 cut(s) 257
BseAI TCCGGA 1 cut(s) 308
BseBI CCWGG 1 cut(s) 319
BseJI GATNNNNATC 1 cut(s) 257
BseLI CCNNNNNNNGG 3 cut(s) 44, 308, 337
BseRI GAGGAG 1 cut(s) 24
BshNI GGYRCC 1 cut(s) 47
BsiHKAI GWGCWC 1 cut(s) 328
BsiSI CCGG 1 cut(s) 309
BslI CCNNNNNNNGG 3 cut(s) 44, 308, 337
BsmAI GTCTC 1 cut(s) 139
Bsp1286I GDGCHC 1 cut(s) 328
Bsp13I TCCGGA 1 cut(s) 308
Bsp143I GATC 2 cut(s) 305, 314
BspEI TCCGGA 1 cut(s) 308
BspLI GGNNCC 2 cut(s) 49, 316
BspPI GGATC 3 cut(s) 300, 309, 322
BspT107I GGYRCC 1 cut(s) 47
BssMI GATC 2 cut(s) 305, 314
Bst2UI CCWGG 1 cut(s) 319
Bst4CI ACNGT 2 cut(s) 166, 292
Bst6I CTCTTC 1 cut(s) 88
BstBAI YACGTR 1 cut(s) 337
BstC8I GCNNGC 2 cut(s) 229, 328
BstDEI CTNAG 1 cut(s) 281
BstHHI GCGC 1 cut(s) 44
BstKTI GATC 2 cut(s) 308, 317
BstMAI GTCTC 1 cut(s) 139
BstMBI GATC 2 cut(s) 305, 314
BstNI CCWGG 1 cut(s) 319
BstNSI RCATGY 1 cut(s) 231
BstSCI CCNGG 1 cut(s) 317
BstX2I RGATCY 1 cut(s) 314
BstYI RGATCY 1 cut(s) 314
Cac8I GCNNGC 2 cut(s) 229, 328
CfoI GCGC 1 cut(s) 44
CviAII CATG 1 cut(s) 228
DdeI CTNAG 1 cut(s) 281
DpnI GATC 2 cut(s) 307, 316
DpnII GATC 2 cut(s) 305, 314
DriI GACNNNNNGTC 1 cut(s) 127
Eam1104I CTCTTC 1 cut(s) 88
Eam1105I GACNNNNNGTC 1 cut(s) 127
EarI CTCTTC 1 cut(s) 88
Eco32I GATATC 1 cut(s) 259
Eco72I CACGTG 1 cut(s) 337
EcoRII CCWGG 1 cut(s) 317
EcoRV GATATC 1 cut(s) 259
FaeI CATG 1 cut(s) 231
FaiI YATR 3 cut(s) 196, 198, 229
FatI CATG 1 cut(s) 227
GlaI GCGC 1 cut(s) 43
HapII CCGG 1 cut(s) 309
HhaI GCGC 1 cut(s) 44
Hin1II CATG 1 cut(s) 231
Hin6I GCGC 1 cut(s) 42
HinP1I GCGC 1 cut(s) 42
HincII GTYRAC 1 cut(s) 295
HindII GTYRAC 1 cut(s) 295
HinfI GANTC 3 cut(s) 128, 182, 277
HpaII CCGG 1 cut(s) 309
Hpy166II GTNNAC 1 cut(s) 295
Hpy188III TCNNGA 2 cut(s) 138, 309
Hpy8I GTNNAC 1 cut(s) 295
HpyAV CCTTC 4 cut(s) 61, 68, 75, 79
HpyCH4III ACNGT 2 cut(s) 166, 292
HpyCH4IV ACGT 2 cut(s) 336, 347
HpyCH4V TGCA 2 cut(s) 206, 231
HpyF3I CTNAG 1 cut(s) 281
HpySE526I ACGT 2 cut(s) 336, 347
Hsp92II CATG 1 cut(s) 231
HspAI GCGC 1 cut(s) 42
Kpn2I TCCGGA 1 cut(s) 308
Kzo9I GATC 2 cut(s) 305, 314
LmnI GCTCC 1 cut(s) 224
LpnPI CCDG 5 cut(s) 30, 123, 304, 322, 331
LweI GCATC 1 cut(s) 215
MaeII ACGT 2 cut(s) 336, 347
MaeIII GTNAC 3 cut(s) 190, 332, 343
MalI GATC 2 cut(s) 307, 316
MboI GATC 2 cut(s) 305, 314
MboII GAAGA 3 cut(s) 75, 184, 246
MflI RGATCY 1 cut(s) 314
MhlI GDGCHC 1 cut(s) 328
MluCI AATT 1 cut(s) 175
MlyI GAGTC 1 cut(s) 137
MnlI CCTC 5 cut(s) 34, 45, 251, 259, 305
MroI TCCGGA 1 cut(s) 308
MroXI GAANNNNTTC 1 cut(s) 8
MspI CCGG 1 cut(s) 309
MspR9I CCNGG 1 cut(s) 319
MvaI CCWGG 1 cut(s) 319
NdeII GATC 2 cut(s) 305, 314
NlaIII CATG 1 cut(s) 231
NlaIV GGNNCC 2 cut(s) 49, 316
NmuCI GTSAC 2 cut(s) 332, 343
NspI RCATGY 1 cut(s) 231
PaeI GCATGC 1 cut(s) 231
PdmI GAANNNNTTC 1 cut(s) 8
PfeI GAWTC 2 cut(s) 182, 277
PleI GAGTC 1 cut(s) 136
PmaCI CACGTG 1 cut(s) 337
PmlI CACGTG 1 cut(s) 337
PpsI GAGTC 1 cut(s) 136
Ppu21I YACGTR 1 cut(s) 337
Psp6I CCWGG 1 cut(s) 317
PspCI CACGTG 1 cut(s) 337
PspGI CCWGG 1 cut(s) 317
PspN4I GGNNCC 2 cut(s) 49, 316
PsuI RGATCY 1 cut(s) 314
Sau3AI GATC 2 cut(s) 305, 314
SchI GAGTC 1 cut(s) 137
ScrFI CCNGG 1 cut(s) 319
SduI GDGCHC 1 cut(s) 328
SetI ASST 4 cut(s) 26, 323, 339, 350
SfaNI GCATC 1 cut(s) 215
SphI GCATGC 1 cut(s) 231
Sse9I AATT 1 cut(s) 175
SspI AATATT 1 cut(s) 9
StyD4I CCNGG 1 cut(s) 317
TaaI ACNGT 2 cut(s) 166, 292
TaiI ACGT 2 cut(s) 339, 350
TaqI TCGA 1 cut(s) 209
TasI AATT 1 cut(s) 175
TfiI GAWTC 2 cut(s) 182, 277
TseFI GTSAC 2 cut(s) 332, 343
Tsp45I GTSAC 2 cut(s) 332, 343
TspDTI ATGAA 2 cut(s) 17, 231
XceI RCATGY 1 cut(s) 231
XmnI GAANNNNTTC 1 cut(s) 8
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.