RLG00000032169

Protein BREAST CANCER SUSCEPTIBILITY 1 homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0008716
Physical Location & Seq
Forward (+)
11771913 .. 11777906
5994 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000032169

Sequence Viewer

Length: 3276 bp
ATGGGCAATCTGGCGCACCTCGAGAAAATGGGCAGAGAACTCAAATGCCCAATATGTTTGAGTCTGTTTAGTTCAGCCGTTTCGCTGAATTGCAATCATGTCTTCTGCAACTCTTGTATTGTGAAATCAATGAAATCGGGTTACAATTGTCCTGTTTGTAAGATCCCATATCAGCGGAGAGAGGTTCGCCCTGCTCCTCATATGGACAATTTGGTTAGTATTTACAAAAGCATGGAGGATGCCTCGGGTATTAACATGTTTATGACTCAAAGTGTGCTGTCAGCTAAATCATCAGATGGAAAGCAGCAAGCTGATGATGAGAACCCTGATGATCAAGATCCTCCTAAACTTTCTCAGAATAGAGCTGGGAACGGGAGAACCGTAAGAGGGAAAAGATCATGGAAAACTAAATCCAATCTAAAGAACTCTGAATCTGTTTCGGTTAAGCCTTCATTTCCAACCAAGAAAAGGGTTCAGGTTCCACAATGTCCTCCTTCAGAAACCCCTACACGGCCTAAACAGTTACCGGGTGGTTTTATTGATGAAAAGTCGAAGCAGGAGTCTACAACTCTAAAGGAGAAGCCTGTTTTAAATGAAAAGGGAGAACCGGTGCTTTCACCTTTCTTTTGGTTGAGTGACAAAGATGCTGAAAATCTGAGTGAGCCTTCTAGTGGTGATCAGCTTAATGATATTCCATCTCCAAATGTTCCTACTTTCAGTGATATCAAGGACTCAGATGATGAAGACTGTTCTAGATTGAGCCCACTGGGCAAAGTGCAGGGGAAATCTTCCAATGTTGCTGATTTTTTTGATAGTGAAATGTTTGAATGGACGCAAAGGCCGTGCTCCCCTGAGCTCTTTGCAAGTCCTTCTAAGATGCAGGTTGTGGATAGTGATCAAATTGACAGAATTCAAGTGAAGGAACTAAAAGAAGCTTTACAAAACAAGAAAATAGGTGAACAAGTCACTGCTGAAAAAGCAAGGTCCAGAAATTCTAGACAGAGTAGTGGAAACTTAATCTTGCTGCCTGATCTTCCACTTGATAAAACTGAAGATGCTAATCATCAATTTCTTTGCAAACCATCTAACAAGAGAGGCAGACAAGCAAGAAAAATAGCTAAAACAAAGTGTACTACAAGTCTTCTGGACCTGGTTGAGAATAGTAGTGCTAATTTGAAGGGTTCTGAAGATTTTTATCAAGAACACGGCTGCAAGAATAGTGACAGTTCTATGGCCAAGGCTGGCAAAAGGAATAAGAAAGCTCATCCATCTCGGCTCGCAACTAAGCCAACATGTAAAAGTGTTTGCATCCTCTCTATTGGTGCTGAAAGGCAGAATGAAGGCAGTGCTGAGAAGGAGTTAACTGAATTACCTACTTCACTAGAAAAGAATGAAGGCAGTGATGAAGCTTTTGCCCAGGAGAAGGCTAAGAAAATATGTACGGAGATAAATGCCAAGAATCAGATGTGTCGCTCTGTAAGGTCGAGGAAACAAAAGGTGGTCTCTATGCCCAACATACTCGATGAGGTATCGGAAATCAAGAAGCAAGCAAATAAGAATACTACAACTGAGTCGTCCCATGTAACTGTCCCTACAGATGATAACAGGAAAGTCTCTGACGTCAAGAATATATCCGCAAAACTTGCTAGTGAAGCCAAGTCATCTGATCGAGAATTGAAATTCAACAAAAGGGTCAGAGTTTCCTCTGGTGATGACTCAAAGGATAAGGAGGTATCAGAGATCAAGAAGCAAGCAAATAAGAATGCTCCAACTGAGTTGCCCCTTATAAATCTCCCTACAAATGTTAACAGGGAACTATCAGGAGTCGGAAAACGTGCTAGAGAAGCCAAGTCATGTGATCGAAAAACTACCAAAAAGTTGAGAGTTTCCTTTGATGACAATTCAAAAGATGAGGCTGTTATTGAGATTCGAGAGGGTCAACATAATGTTAATGAAAATGGTAACCAACCCTCTGAGAAGAGCAAAGGCAATTCCAATGCTAGACTTTTTACTGATCGATCATCAATGCAGAAGCTTCCTACGTTGAAGAATGATGTGGTTTTGAGGAGATGTGATGCCGTTACTAGTAACATTCAATGTGCTTTTTGTCTTTCATCAGAAGAATCAGAGGCATCAGGAGAAATAGTCCACTATTATAATGGCAAGCCTGTTGCTGCAGATCACAATAGAGGGTCCAAGGTTATACATTCACACAGGAACTGCACTGAATGGGCTCCCAACGTATATTTTGAAGATGATATTGCTATGAATCTTGAAGCTGAGTTAACTAGAAGTCGTAGAATTAAATGTTGTTGCTGTGGAATTAAAGGAGCATCTCTTGGGTGTTATGAGAAGAGTTGTCGTAAGAGCTTTCATGTTAGCTGTGCAAAGATGACTCCCGAATGCCGATGGGATTCTGATAATTTTGTTATGTTATGCCCCATTCATGCCTCTTCTAAGTTGCCGAATGAAAGTTCTGAATCTGAAGCAAGGAGGAAGAAGAGCAACCCTAGAAAACAACCTGGTGCTGATCATAATAAAGTTGCTGTTAAACAAGACAGCAATACCTGTCAGGATCTGAAATTCTGTGGATCATCCAAGAAATTGGTTCTCTGTTGTTCAACTCTCACAAATGCAGAGCGGGAATATGTTGCAGAATTTGAAAAATTATCTGGAGTTACAGTCTTAAAGAAGTGGGACTCTAATATCACACATATTGTTGCGCCAATAGATGAAAATGGAGCATGCAGAAGGACCCTCAAAGTATTGTTGGGAATCTTGGAGGGGAAGTGGATACTGAGCATGGGATGGATTAAAGCTTGCATGGAAGCCATGAAACTAGTGAATGAGGAGCCTTATGAAATTAGTGTTGACATTTATGGAATCAGGGATGGCCCTCGACTTGGTAGACTAAGACTGCAGAACAAGGAACCAAAGCTTTTTGATGGGCTTAAGTTTTACTTCATGGGAGATTTTGTACCTTCATACAAGGGGTATCTACAAGACATTGTAATAGCTGCTGGAGGAACTGTTTTGCACAGAAAGCCAGTTCCAGAGAGCCAGAAAGGGTCCTCTGGAAGTCCCCTCGAATGTCAAACCTTCATCATCTTTAGTCTTGAGCTACCTGATCAATGTCATCCTAGCAAGAAGGGTACCATTTTCAACCAAAGGCAGGCGGATGCAAAGTCTTTGGCAAGTTCTGCTGGAGCCAATGTAGTAAGCAATTCATGGATTTTGAACTCCGTTGCAGCCTGCAAGTTCCAAAGTCTTTCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000151 GO:0000152 GO:0000724 GO:0000725 GO:0003674 GO:0003824 GO:0004842 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005886 GO:0006139 GO:0006259 GO:0006281 GO:0006302 GO:0006310 GO:0006355 GO:0006357 GO:0006464 GO:0006725 GO:0006807 GO:0006950 GO:0006974 GO:0008150 GO:0008152 GO:0009314 GO:0009628 GO:0009889 GO:0009890 GO:0009891 GO:0009892 GO:0009893 GO:0009987 GO:0010212 GO:0010332 GO:0010468 GO:0010556 GO:0010557 GO:0010564 GO:0010565 GO:0010604 GO:0010605 GO:0010628 GO:0010638 GO:0010639 GO:0016020 GO:0016567 GO:0016740 GO:0019216 GO:0019217 GO:0019219 GO:0019222 GO:0019538 GO:0019787 GO:0031056 GO:0031057 GO:0031058 GO:0031323 GO:0031324 GO:0031325 GO:0031326 GO:0031327 GO:0031328 GO:0031399 GO:0031400 GO:0031401 GO:0031436 GO:0032268 GO:0032269 GO:0032270 GO:0032446 GO:0032991 GO:0033043 GO:0033044 GO:0033554 GO:0034641 GO:0035065 GO:0035066 GO:0035067 GO:0036211 GO:0042127 GO:0042304 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044464 GO:0045717 GO:0045787 GO:0045833 GO:0045893 GO:0045922 GO:0045935 GO:0045944 GO:0046483 GO:0046890 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0050789 GO:0050794 GO:0050896 GO:0051055 GO:0051128 GO:0051129 GO:0051130 GO:0051171 GO:0051172 GO:0051173 GO:0051246 GO:0051247 GO:0051248 GO:0051252 GO:0051254 GO:0051716 GO:0051726 GO:0060255 GO:0062012 GO:0062014 GO:0065007 GO:0070531 GO:0070647 GO:0071156 GO:0071158 GO:0071214 GO:0071478 GO:0071479 GO:0071480 GO:0071704 GO:0071944 GO:0080090 GO:0090068 GO:0090304 GO:0104004 GO:0140096 GO:1901360 GO:1901564 GO:1901983 GO:1901984 GO:1901985 GO:1902275 GO:1902494 GO:1902680 GO:1903506 GO:1903508 GO:1905268 GO:1905269 GO:1990234 GO:2000112 GO:2000756 GO:2000757 GO:2000758 GO:2001141 GO:2001251 GO:2001252
KEGG Pathways
Metabolic & Signaling
ko03440 map03440
Pfam Domains
Protein Families
BRCT BRCT_2 DYW_deaminase PPR zf-C3HC4_2 zf-HC5HC2H zf-RING_2

Protein Analysis

1092

Amino Acids

121.12

Weight (kDa)

8.93

Isoelectric Point (pI)

51.83

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-RING_6 PF14835 4 - 69 7.4e-06 zf-RING of BARD1-type protein
zf-C3HC4_3 PF13920 13 - 59 8.9e-07 Zinc finger, C3HC4 type (RING finger)
zf-C3HC4_2 PF13923 15 - 53 4.3e-10 Zinc finger, C3HC4 type (RING finger)
zf-RING_2 PF13639 15 - 54 1.3e-07 Ring finger domain
zf-RING_UBOX PF13445 16 - 51 4.2e-08 RING-type zinc-finger
zf-C3HC4 PF00097 16 - 53 5.1e-08 Zinc finger, C3HC4 type (RING finger)
zf-C3HC4_4 PF15227 16 - 53 8.8e-07 zinc finger of C3HC4-type, RING
zf-HC5HC2H_2 PF13832 736 - 798 9.7e-06 PHD-zinc-finger like domain
zf-HC5HC2H PF13771 738 - 816 3.8e-09 PHD-like zinc-binding domain
BRCT PF00533 868 - 942 9.6e-09 BRCA1 C Terminus (BRCT) domain
BRCT_2 PF16589 979 - 1088 3.2e-07 BRCT domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 1787, 2157
AatII GACGTC 1 cut(s) 1623
Acc36I ACCTGC 1 cut(s) 871
Acc65I GGTACC 1 cut(s) 3152
AccB1I GGYRCC 1 cut(s) 3152
AccBSI CCGCTC 1 cut(s) 2641
AccI GTMKAC 2 cut(s) 563, 2908
AciI CCGC 4 cut(s) 175, 1635, 2641, 3176
AclWI GGATC 4 cut(s) 157, 332, 2583, 2599
AcoI YGGCCR 1 cut(s) 1233
AcsI RAATTY 5 cut(s) 909, 991, 1679, 2582, 2657
AcuI CTGAAG 4 cut(s) 480, 1071, 1206, 2505
AcyI GRCGYC 1 cut(s) 1620
AfaI GTAC 4 cut(s) 1132, 1441, 2979, 3154
AfiI CCNNNNNNNGG 7 cut(s) 387, 468, 510, 671, 1423, 2903, 3172
AflII CTTAAG 1 cut(s) 2951
AflIII ACRYGT 2 cut(s) 255, 1292
AgeI ACCGGT 1 cut(s) 607
AhlI ACTAGT 2 cut(s) 2084, 2839
AjnI CCWGG 3 cut(s) 1149, 1416, 2521
Alw21I GWGCWC 2 cut(s) 848, 858
Alw26I GTCTC 2 cut(s) 1507, 1618
AlwI GGATC 4 cut(s) 157, 332, 2583, 2599
AlwNI CAGNNNCTG 2 cut(s) 2220, 2578
Ama87I CYCGRG 2 cut(s) 20, 244
AoxI GGCC 4 cut(s) 512, 839, 1233, 2893
ApeKI GCWGC 6 cut(s) 304, 1024, 1209, 2174, 3017, 3248
ApoI RAATTY 5 cut(s) 909, 991, 1679, 2582, 2657
ArsI GACNNNNNNTTYG 2 cut(s) 545, 577
AsiGI ACCGGT 1 cut(s) 607
Asp718I GGTACC 1 cut(s) 3152
AspLEI GCGC 2 cut(s) 16, 2725
AspS9I GGNCC 6 cut(s) 984, 1147, 2193, 2754, 2894, 3069
AsuC2I CCSGG 1 cut(s) 528
AsuHPI GGTGA 4 cut(s) 609, 686, 968, 1721
AvaI CYCGRG 2 cut(s) 20, 244
AvaII GGWCC 5 cut(s) 984, 1147, 2193, 2754, 3069
BalI TGGCCA 1 cut(s) 1235
BanI GGYRCC 1 cut(s) 3152
BanII GRGCYC 3 cut(s) 764, 858, 2236
BbsI GAAGAC 3 cut(s) 94, 750, 1133
Bbv12I GWGCWC 2 cut(s) 848, 858
BbvI GCAGC 6 cut(s) 316, 1011, 1196, 2161, 3004, 3260
BccI CCATC 8 cut(s) 290, 703, 1090, 1276, 2403, 2802, 2885, 2939
BceAI ACGGC 5 cut(s) 62, 527, 826, 1222, 2063
BciT130I CCWGG 3 cut(s) 1151, 1418, 2523
BciVI GTATCC 1 cut(s) 2787
BclI TGATCA 5 cut(s) 331, 676, 895, 2530, 3127
BcnI CCSGG 1 cut(s) 528
BcoDI GTCTC 2 cut(s) 1507, 1618
BcuI ACTAGT 2 cut(s) 2084, 2839
BfmI CTRYAG 3 cut(s) 1593, 2175, 2918
BfrI CTTAAG 1 cut(s) 2951
BfuAI ACCTGC 1 cut(s) 871
BfuI GTATCC 1 cut(s) 2787
BglI GCCNNNNNGGC 1 cut(s) 768
BisI GCNGC 6 cut(s) 305, 1025, 1210, 2175, 3018, 3249
BlsI GCNGC 6 cut(s) 306, 1026, 1211, 2176, 3019, 3250
Bme1390I CCNGG 4 cut(s) 528, 1151, 1418, 2523
Bme18I GGWCC 5 cut(s) 984, 1147, 2193, 2754, 3069
BmeT110I CYCGRG 2 cut(s) 20, 244
BmgT120I GGNCC 6 cut(s) 984, 1147, 2193, 2754, 2894, 3069
BmiI GGNNCC 9 cut(s) 480, 2194, 2235, 2756, 2853, 2931, 3070, 3154, 3208
BmrFI CCNGG 4 cut(s) 528, 1151, 1418, 2523
BmrI ACTGGG 1 cut(s) 776
BmsI GCATC 9 cut(s) 229, 634, 867, 1045, 1317, 2065, 2141, 2342, 3169
BmuI ACTGGG 1 cut(s) 776
BpiI GAAGAC 3 cut(s) 94, 750, 1133
BplI GAGNNNNNCTC 2 cut(s) 227, 259
BpmI CTGGAG 3 cut(s) 2694, 3042, 3225
Bpu10I CCTNAGC 1 cut(s) 852
BpuEI CTTGAG 1 cut(s) 3137
BpuMI CCSGG 1 cut(s) 528
Bsa29I ATCGAT 1 cut(s) 2017
BsaBI GATNNNNATC 4 cut(s) 336, 894, 1059, 1194
BsaHI GRCGYC 1 cut(s) 1620
BsaI GGTCTC 1 cut(s) 1507
BsaJI CCNNGG 4 cut(s) 243, 1236, 1416, 2196
BsaWI WCCGGW 1 cut(s) 607
Bsc4I CCNNNNNNNGG 7 cut(s) 387, 468, 510, 671, 1423, 2903, 3172
Bse118I RCCGGY 1 cut(s) 607
Bse1I ACTGG 2 cut(s) 771, 3047
Bse8I GATNNNNATC 4 cut(s) 336, 894, 1059, 1194
BseBI CCWGG 3 cut(s) 1151, 1418, 2523
BseCI ATCGAT 1 cut(s) 2017
BseDI CCNNGG 4 cut(s) 243, 1236, 1416, 2196
BseGI GGATG 8 cut(s) 244, 1264, 1308, 2594, 2813, 2896, 3136, 3184
BseJI GATNNNNATC 4 cut(s) 336, 894, 1059, 1194
BseLI CCNNNNNNNGG 7 cut(s) 387, 468, 510, 671, 1423, 2903, 3172
BseNI ACTGG 2 cut(s) 771, 3047
BseRI GAGGAG 3 cut(s) 186, 2080, 2864
BseXI GCAGC 6 cut(s) 316, 1011, 1196, 2161, 3004, 3260
BseYI CCCAGC 1 cut(s) 365
BsgI GTGCAG 2 cut(s) 797, 2206
BshFI GGCC 4 cut(s) 514, 841, 1235, 2895
BshNI GGYRCC 1 cut(s) 3152
BshTI ACCGGT 1 cut(s) 607
BshVI ATCGAT 1 cut(s) 2017
BsiHKAI GWGCWC 2 cut(s) 848, 858
BsiHKCI CYCGRG 2 cut(s) 20, 244
BsiSI CCGG 2 cut(s) 527, 608
BslFI GGGAC 4 cut(s) 1561, 1574, 2711, 3066
BslI CCNNNNNNNGG 7 cut(s) 387, 468, 510, 671, 1423, 2903, 3172
BsmAI GTCTC 2 cut(s) 1507, 1618
BsmFI GGGAC 4 cut(s) 1561, 1574, 2711, 3066
BsmI GAATGC 2 cut(s) 1768, 2408
BsnI GGCC 4 cut(s) 514, 841, 1235, 2895
Bso31I GGTCTC 1 cut(s) 1507
BsoBI CYCGRG 2 cut(s) 20, 244
Bsp1286I GDGCHC 4 cut(s) 764, 848, 858, 2236
BspACI CCGC 4 cut(s) 175, 1635, 2641, 3176
BspANI GGCC 4 cut(s) 514, 841, 1235, 2895
BspDI ATCGAT 1 cut(s) 2017
BspLI GGNNCC 9 cut(s) 480, 2194, 2235, 2756, 2853, 2931, 3070, 3154, 3208
BspMAI CTGCAG 2 cut(s) 2179, 2922
BspMI ACCTGC 1 cut(s) 871
BspPI GGATC 4 cut(s) 157, 332, 2583, 2599
BspQI GCTCTTC 2 cut(s) 1973, 2495
BspT107I GGYRCC 1 cut(s) 3152
BspTI CTTAAG 1 cut(s) 2951
BspTNI GGTCTC 1 cut(s) 1507
BsrBI CCGCTC 1 cut(s) 2641
BsrFI RCCGGY 1 cut(s) 607
BsrI ACTGG 2 cut(s) 771, 3047
BssAI RCCGGY 1 cut(s) 607
BssECI CCNNGG 4 cut(s) 243, 1236, 1416, 2196
BssNI GRCGYC 1 cut(s) 1620
BssT1I CCWWGG 2 cut(s) 1236, 2196
Bst2UI CCWGG 3 cut(s) 1151, 1418, 2523
Bst4CI ACNGT 7 cut(s) 382, 522, 749, 1226, 1588, 2683, 3031
Bst6I CTCTTC 4 cut(s) 1973, 2348, 2458, 2495
BstACI GRCGYC 1 cut(s) 1620
BstAFI CTTAAG 1 cut(s) 2951
BstAPI GCANNNNNTGC 2 cut(s) 1643, 3200
BstEII GGTNACC 1 cut(s) 1961
BstF5I GGATG 8 cut(s) 244, 1264, 1308, 2594, 2813, 2896, 3136, 3184
BstHHI GCGC 2 cut(s) 16, 2725
BstMAI GTCTC 2 cut(s) 1507, 1618
BstMWI GCNNNNNNNGC 7 cut(s) 768, 977, 1643, 1652, 1844, 3043, 3200
BstNI CCWGG 3 cut(s) 1151, 1418, 2523
BstNSI RCATGY 3 cut(s) 259, 1296, 2748
BstPI GGTNACC 1 cut(s) 1961
BstSCI CCNGG 4 cut(s) 526, 1149, 1416, 2521
BstSFI CTRYAG 3 cut(s) 1593, 2175, 2918
BstV1I GCAGC 6 cut(s) 316, 1011, 1196, 2161, 3004, 3260
BstV2I GAAGAC 3 cut(s) 94, 750, 1133
BstX2I RGATCY 3 cut(s) 162, 337, 2575
BstXI CCANNNNNNTGG 1 cut(s) 2605
BstYI RGATCY 3 cut(s) 162, 337, 2575
Bsu15I ATCGAT 1 cut(s) 2017
BsuI GTATCC 1 cut(s) 2787
BsuRI GGCC 4 cut(s) 514, 841, 1235, 2895
BsuTUI ATCGAT 1 cut(s) 2017
BtsCI GGATG 8 cut(s) 244, 1264, 1308, 2594, 2813, 2896, 3136, 3184
BtsI GCAGTG 3 cut(s) 966, 1351, 1405
BtsIMutI CAGTG 6 cut(s) 724, 764, 966, 1351, 1405, 2223
BveI ACCTGC 1 cut(s) 871
CaiI CAGNNNCTG 2 cut(s) 2220, 2578
CfoI GCGC 2 cut(s) 16, 2725
Cfr10I RCCGGY 1 cut(s) 607
Cfr13I GGNCC 6 cut(s) 984, 1147, 2193, 2754, 2894, 3069
ClaI ATCGAT 1 cut(s) 2017
CseI GACGC 1 cut(s) 841
CsiI ACCWGGT 2 cut(s) 1149, 2521
Csp6I GTAC 4 cut(s) 1131, 1440, 2978, 3153
CspAI ACCGGT 1 cut(s) 607
CviQI GTAC 4 cut(s) 1131, 1440, 2978, 3153
DraI TTTAAA 1 cut(s) 591
EaeI YGGCCR 1 cut(s) 1233
Eam1104I CTCTTC 4 cut(s) 1973, 2348, 2458, 2495
EarI CTCTTC 4 cut(s) 1973, 2348, 2458, 2495
EciI GGCGGA 1 cut(s) 3191
Ecl136II GAGCTC 1 cut(s) 856
Eco130I CCWWGG 2 cut(s) 1236, 2196
Eco24I GRGCYC 3 cut(s) 764, 858, 2236
Eco31I GGTCTC 1 cut(s) 1507
Eco32I GATATC 1 cut(s) 724
Eco47I GGWCC 5 cut(s) 984, 1147, 2193, 2754, 3069
Eco53kI GAGCTC 1 cut(s) 856
Eco57I CTGAAG 4 cut(s) 480, 1071, 1206, 2505
Eco88I CYCGRG 2 cut(s) 20, 244
Eco91I GGTNACC 1 cut(s) 1961
EcoICRI GAGCTC 1 cut(s) 856
EcoO109I RGGNCCY 2 cut(s) 2754, 3069
EcoO65I GGTNACC 1 cut(s) 1961
EcoRI GAATTC 1 cut(s) 909
EcoRII CCWGG 3 cut(s) 1149, 1416, 2521
EcoRV GATATC 1 cut(s) 724
EcoT14I CCWWGG 2 cut(s) 1236, 2196
EcoT38I GRGCYC 3 cut(s) 764, 858, 2236
ErhI CCWWGG 2 cut(s) 1236, 2196
FalI AAGNNNNNCTT 3 cut(s) 2945, 2977, 3256
FaqI GGGAC 4 cut(s) 1561, 1574, 2711, 3066
FauI CCCGC 1 cut(s) 2634
FauNDI CATATG 1 cut(s) 201
FbaI TGATCA 5 cut(s) 331, 676, 895, 2530, 3127
FblI GTMKAC 2 cut(s) 563, 2908
Fnu4HI GCNGC 6 cut(s) 305, 1025, 1210, 2175, 3018, 3249
FokI GGATG 8 cut(s) 251, 1251, 1295, 2581, 2820, 2903, 3123, 3191
FriOI GRGCYC 3 cut(s) 764, 858, 2236
Fsp4HI GCNGC 6 cut(s) 305, 1025, 1210, 2175, 3018, 3249
GlaI GCGC 2 cut(s) 15, 2724
GluI GCNGC 6 cut(s) 305, 1025, 1210, 2175, 3018, 3249
GsaI CCCAGC 1 cut(s) 369
GsuI CTGGAG 3 cut(s) 2694, 3042, 3225
HaeIII GGCC 4 cut(s) 514, 841, 1235, 2895
HapII CCGG 2 cut(s) 527, 608
HgaI GACGC 1 cut(s) 841
HhaI GCGC 2 cut(s) 16, 2725
Hin1I GRCGYC 1 cut(s) 1620
Hin6I GCGC 2 cut(s) 14, 2723
HinP1I GCGC 2 cut(s) 14, 2723
HincII GTYRAC 5 cut(s) 1362, 1807, 1940, 2286, 2872
HindII GTYRAC 5 cut(s) 1362, 1807, 1940, 2286, 2872
HindIII AAGCTT 5 cut(s) 933, 1407, 2033, 2817, 2936
HpaI GTTAAC 3 cut(s) 1362, 1807, 2286
HpaII CCGG 2 cut(s) 527, 608
HphI GGTGA 4 cut(s) 609, 686, 968, 1721
HpyCH4III ACNGT 7 cut(s) 382, 522, 749, 1226, 1588, 2683, 3031
HpyCH4IV ACGT 4 cut(s) 1620, 1834, 2042, 2241
HpyF10VI GCNNNNNNNGC 7 cut(s) 768, 977, 1643, 1652, 1844, 3043, 3200
HpySE526I ACGT 4 cut(s) 1620, 1834, 2042, 2241
Hsp92I GRCGYC 1 cut(s) 1620
HspAI GCGC 2 cut(s) 14, 2723
KpnI GGTACC 1 cut(s) 3156
Ksp22I TGATCA 5 cut(s) 331, 676, 895, 2530, 3127
KspAI GTTAAC 3 cut(s) 1362, 1807, 2286
LguI GCTCTTC 2 cut(s) 1973, 2495
LmnI GCTCC 8 cut(s) 199, 851, 1771, 2239, 2330, 2741, 2851, 3206
Lsp1109I GCAGC 6 cut(s) 316, 1011, 1196, 2161, 3004, 3260
LweI GCATC 9 cut(s) 229, 634, 867, 1045, 1317, 2065, 2141, 2342, 3169
MabI ACCWGGT 2 cut(s) 1149, 2521
MaeII ACGT 4 cut(s) 1620, 1834, 2042, 2241
MbiI CCGCTC 1 cut(s) 2641
MfeI CAATTG 1 cut(s) 145
MflI RGATCY 3 cut(s) 162, 337, 2575
MhlI GDGCHC 4 cut(s) 764, 848, 858, 2236
MlsI TGGCCA 1 cut(s) 1235
MluNI TGGCCA 1 cut(s) 1235
MlyI GAGTC 9 cut(s) 70, 259, 569, 725, 1580, 1709, 1833, 2389, 2693
MmeI TCCRAC 3 cut(s) 482, 1793, 1807
Mox20I TGGCCA 1 cut(s) 1235
MscI TGGCCA 1 cut(s) 1235
MslI CAYNNNNRTG 1 cut(s) 260
Msp20I TGGCCA 1 cut(s) 1235
MspA1I CMGCKG 1 cut(s) 175
MspCI CTTAAG 1 cut(s) 2951
MspI CCGG 2 cut(s) 527, 608
MspR9I CCNGG 4 cut(s) 528, 1151, 1418, 2523
MunI CAATTG 1 cut(s) 145
Mva1269I GAATGC 2 cut(s) 1768, 2408
MvaI CCWGG 3 cut(s) 1151, 1418, 2523
MwoI GCNNNNNNNGC 7 cut(s) 768, 977, 1643, 1652, 1844, 3043, 3200
NciI CCSGG 1 cut(s) 528
NdeI CATATG 1 cut(s) 201
NlaIV GGNNCC 9 cut(s) 480, 2194, 2235, 2756, 2853, 2931, 3070, 3154, 3208
NmeAIII GCCGAG 1 cut(s) 1252
NmuCI GTSAC 3 cut(s) 635, 964, 1220
NspI RCATGY 3 cut(s) 259, 1296, 2748
PaeI GCATGC 1 cut(s) 2748
PaeR7I CTCGAG 1 cut(s) 20
PciI ACATGT 2 cut(s) 255, 1292
PciSI GCTCTTC 2 cut(s) 1973, 2495
PcsI WCGNNNNNNNCGW 2 cut(s) 378, 839
PctI GAATGC 2 cut(s) 1768, 2408
PfeI GAWTC 9 cut(s) 431, 1459, 1927, 2123, 2269, 2414, 2480, 2775, 2883
PinAI ACCGGT 1 cut(s) 607
PkrI GCNGC 6 cut(s) 306, 1026, 1211, 2176, 3019, 3250
PleI GAGTC 9 cut(s) 69, 259, 568, 725, 1579, 1709, 1832, 2389, 2693
PpsI GAGTC 9 cut(s) 69, 259, 568, 725, 1579, 1709, 1832, 2389, 2693
PpuMI RGGWCCY 2 cut(s) 2754, 3069
PscI ACATGT 2 cut(s) 255, 1292
PsiI TTATAA 2 cut(s) 1787, 2157
Psp124BI GAGCTC 1 cut(s) 858
Psp5II RGGWCCY 2 cut(s) 2754, 3069
Psp6I CCWGG 3 cut(s) 1149, 1416, 2521
PspEI GGTNACC 1 cut(s) 1961
PspFI CCCAGC 1 cut(s) 365
PspGI CCWGG 3 cut(s) 1149, 1416, 2521
PspN4I GGNNCC 9 cut(s) 480, 2194, 2235, 2756, 2853, 2931, 3070, 3154, 3208
PspPI GGNCC 6 cut(s) 984, 1147, 2193, 2754, 2894, 3069
PspPPI RGGWCCY 2 cut(s) 2754, 3069
PstI CTGCAG 2 cut(s) 2179, 2922
PstNI CAGNNNCTG 2 cut(s) 2220, 2578
PsuI RGATCY 3 cut(s) 162, 337, 2575
RsaI GTAC 4 cut(s) 1132, 1441, 2979, 3154
RsaNI GTAC 4 cut(s) 1131, 1440, 2978, 3153
RseI CAYNNNNRTG 1 cut(s) 260
SacI GAGCTC 1 cut(s) 858
SapI GCTCTTC 2 cut(s) 1973, 2495
SatI GCNGC 6 cut(s) 305, 1025, 1210, 2175, 3018, 3249
Sau96I GGNCC 6 cut(s) 984, 1147, 2193, 2754, 2894, 3069
SchI GAGTC 9 cut(s) 70, 259, 569, 725, 1580, 1709, 1833, 2389, 2693
ScrFI CCNGG 4 cut(s) 528, 1151, 1418, 2523
SduI GDGCHC 4 cut(s) 764, 848, 858, 2236
SexAI ACCWGGT 2 cut(s) 1149, 2521
SfaNI GCATC 9 cut(s) 229, 634, 867, 1045, 1317, 2065, 2141, 2342, 3169
SfcI CTRYAG 3 cut(s) 1593, 2175, 2918
Sfr274I CTCGAG 1 cut(s) 20
SinI GGWCC 5 cut(s) 984, 1147, 2193, 2754, 3069
SlaI CTCGAG 1 cut(s) 20
SmiMI CAYNNNNRTG 1 cut(s) 260
SmlI CTYRAG 3 cut(s) 20, 2951, 3116
SmoI CTYRAG 3 cut(s) 20, 2951, 3116
SpeI ACTAGT 2 cut(s) 2084, 2839
SphI GCATGC 1 cut(s) 2748
SsiI CCGC 4 cut(s) 175, 1635, 2641, 3176
SstI GAGCTC 1 cut(s) 858
StyD4I CCNGG 4 cut(s) 526, 1149, 1416, 2521
StyI CCWWGG 2 cut(s) 1236, 2196
TaaI ACNGT 7 cut(s) 382, 522, 749, 1226, 1588, 2683, 3031
TaiI ACGT 4 cut(s) 1623, 1837, 2045, 2244
TatI WGTACW 1 cut(s) 1130
TfiI GAWTC 9 cut(s) 431, 1459, 1927, 2123, 2269, 2414, 2480, 2775, 2883
TscAI CASTG 6 cut(s) 724, 771, 973, 1351, 1405, 2230
TseFI GTSAC 3 cut(s) 635, 964, 1220
TseI GCWGC 6 cut(s) 304, 1024, 1209, 2174, 3017, 3248
Tsp45I GTSAC 3 cut(s) 635, 964, 1220
TspGWI ACGGA 2 cut(s) 1457, 3232
TspRI CASTG 6 cut(s) 724, 771, 973, 1351, 1405, 2230
Vha464I CTTAAG 1 cut(s) 2951
VpaK11BI GGWCC 5 cut(s) 984, 1147, 2193, 2754, 3069
XapI RAATTY 5 cut(s) 909, 991, 1679, 2582, 2657
XbaI TCTAGA 2 cut(s) 752, 995
XceI RCATGY 3 cut(s) 259, 1296, 2748
XcmI CCANNNNNNNNNTGG 1 cut(s) 2156
XhoI CTCGAG 1 cut(s) 20
XmiI GTMKAC 2 cut(s) 563, 2908
ZraI GACGTC 1 cut(s) 1621
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.