Rh5BG116500

Protein BREAST CANCER SUSCEPTIBILITY 1 homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5B
OG0008716
Physical Location & Seq
Forward (+)
10972920 .. 10978762
5843 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5BG116500.1

Sequence Viewer

Length: 3267 bp
ATGGGCAATCTGGCGCACCTCGAGAAAATGGGCAGAGAACTCAAATGCCCAATATGTTTGAGTCTGTTTAGTTCAGCCGTTTCGCTGAATTGCAATCATGTCTTCTGCAACTCTTGTATTGTGAAATCAATGAAATCGGGTTCCAATTGTCCTGTTTGTAAGATCCCATTTCAGCGGAGAGAGGTTCGCCCTGCTCCTCATATGGACAATTTGGTTAGTATTTACAAAAGCATGGAGGATGCTTCGGGTATTAACATGTTTATGACTCAAAGTGCGCTGTCAGCTAAATCATCAGATGGAAAGCAGCAAGCTGAAGATGAGAACCCTGATGATCAAGATCCTCCTAAACTTTCTCAGAATAGAGCTGGGAACAGGAGAACCGTAAGAGGGAAAAGATCTGAATCCAATCTAAAGAACTCTGAATCTGTTTCGGTTAAGCCTTCATTTCCAACCAAGAAAAGGGTTCAGGTTCCACAATGTCCTCCTTCAGAAACCCCTACACGGCCTAAACAGTTACCGGGTGGTTTTATTGATGAAAAGTCGAAGCAGGAGTCTACAACACTAAAGGAGAAGCCTGTTTTAAATGAAAAGGGAGAACTGGTGCTTTCACCTTTCTTTTGGTTGAGTGACAAAGATGCTGAAAATTTGAGTCAACCTTCTAGTGGTGATCAGCTTAATGATATTCCATCTCCCAATGTTCCTACTTTCAGTGATATCAAGGACTCAGATGATGAAGACTGTTCTAGATTGAGCCCACTGGGCAAAGTGCAGGGGAAATCTTCCAATGTTGCTGATTTTTTTGATAGTGAAATGTTCGAATGGACGCAAAGGCCGTGCTCCCCTGAGCTCTTTGCAAGTCCTTCTAAGATGCAGGTTGTGGATAGTGATCAAATTGATAGAATTCAAGTGAAGGAACTAAAAGAAGCTTTACTAAACAAGAAAATAGGTGAACAAGTCACTGCTGAAAAAGAAAGGTCCAGAAATTCTAGACAGAGTAGTGGAAACTTAATCTTGCTGCCTGATCTTCCACATGATAAAACTGAAGATGCTAATCATCAATTTCTTTGCAAACCATCTAACAAGAGAGGCAGACAAGCAAGAAAAATAGCTAAAAGAAAGTGTGCTACAAGTCATATGGACCTGGTTGAGAATAGTAGTGCTAATTTGAAGGGTTCTGAAGATTTTTATCAAGAACACGGCTGCAAGAATAGTGACAGTTCTATGGCCAAGGCTGGCAAAAGGAAGAAGAAAGCTCATCTGTCTCGGCTCGCAACTAAGCCAACATGTGAAAGTGTTTGCATCCTCTCTATTGGTGCTGAAAGGCAGAATGAAGGCAGTGCTGAGAAGGGGTTAACTGAATTACCTACTTCACTAGAAAAGAATGAAGGCAGTGATGAAGCTTTTGCCCAAGAGAAGGCTAAGAAAATATGTACGGAGATAAATGCCAAGAACCAGATGTGTCGCTCTGTAAGGTCAAGGAAACAAAAGGTGGTCTCTATGCCCAACATACTCGATGAGGTATCGGAAATCAAGAAGCAAGCAAATAAGAATACTACAACTGAGTCGTCCCATGTAACTGTCCCTACAGATGATAACAGGAAAGTCTCTGACGTCAAGAATATATCCACAAAACTTGCTAGCGAAGCCAAGTCATCTGATCGAGAATTGAAATTCAACAAAAGGGTCAGAGTTTCCTCTGGTGATGACTCAAAGGATAAGGAGGTATCAGAGATCAAGAAGCAAGCAAATAAGAATGCTCCAACTGAGTTGCCCCTTAGAAATCTCCCTACAAATGTTAGCAGGGAACTATCAGGAGTCAGAAAACGTGCTAGAGAAACCAAGTCATGTGATCGAAAAACTACCAAAAAGTTGAGAGTTTCCTTTGATGACAGTTCAAAAGATGAGGCTGTTATTGAGATTCGAGAGGGTCAACATAATGTTAATGAAAATAGTAACCAACCCTCCAAGAAGAGCAAAGGCAATTCCAATGCTAGAATTTTTACTGACCGATCATCAACGCAGAAGCTTCCTACATTGAAGAATGATGTGGTTTTGAGGAGATGTGATGCCGTTACTAGTAACATTCAATGTGCCTTTTGTCTTTCATCAGAAGAATCAGAGGCATCAGGAGAAATAGTTCACTATTATAATGGCAAGCCTGTTGCTGCAGATCACAATGGGGGGTCCAAGGTTATACATTCACACAGGAACTGCACTGAATGGGCTCCCAACGTATATTTTGAAGATGATATTGCTATGAATCTTGAAGCTGAGCTAACTAGAAGTCGTAGAATTAAATGTTGTTGCTGTGGAATTAAAGGAGCATCTCTTGGGTGTTATGAGAAGAGTTGTCGCAAGAGCTTTCATGTTAGCTGTGCAAAGATGACTCCTGAATGTCGATGGGATTCTGATAATTTCGTTATGTTATGCCCCATTCATGCCTCTTCTAAGTTGCCAAATGAAAGTTCTGAATCTGAAGCAAGGAGGAAGAAGAGCAACCCTAGAAAACAACCTGGTGCTGATCATAATAAAGTTGCTGTTAAACAAGACAGCAATACCTGTCAGGATCTGAAATTTTGTGGATCATCCAAGAAATTGGTTCTCTGTTGTTCAACTCTCACAAATGCAGAGCGGGAATATGTTGCAGAATTTGAAAAATTATCTGGAGTTACAGTCTTAAAGAAGTGGGACTCTAGTATCACACATATTGTTGCGCCAATAGATGAAAATGGAGCATGCAGAAGAACCCTCAAAGTATTGTTGGGAATCTTGGAGGGGAAGTGGATACTGAGCATGGGATGGATTAAAGCTTGCATGGAAGCCATGAAACTAGTGAATGAGGAGCCTTATGAAATTAGCGTTGACATTTATGGAATCAGGGATGGCCCTCGACTTGGTAGACTAAGACTGCAGAACAAGGAACCAAAGCTTTTTGATGGGCTTAAGTTTTACTTCATGGGAGATTTTGTACCTTCATACAAGGGGTATCTACAAGACCTTGTAATAGCTGCTGGAGGAACTGTTTTGCACAGAAAGCCTGTTCCAGAGAGCCAGAAAGGGTCGTCTGGAAGCCCCCTCGAATGTCGAACCTTCATTATCTTTAGTCTTGAGCTACCTGATCAATGTCATCCTAGCAAGAAGGGTACCATTTTCAACCAAAGGCAGGCGGATGCAAAGTCTTTGGCAAGTTCTGCTGGAGCCAATGTAGTAAGCAATTCATGGATTTTGAACTCTGTTGCAGCCTGCAAGTTACAAAATCTCTCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000151 GO:0000152 GO:0000724 GO:0000725 GO:0003674 GO:0003824 GO:0004842 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005886 GO:0006139 GO:0006259 GO:0006281 GO:0006302 GO:0006310 GO:0006355 GO:0006357 GO:0006464 GO:0006725 GO:0006807 GO:0006950 GO:0006974 GO:0008150 GO:0008152 GO:0009314 GO:0009628 GO:0009889 GO:0009890 GO:0009891 GO:0009892 GO:0009893 GO:0009987 GO:0010212 GO:0010332 GO:0010468 GO:0010556 GO:0010557 GO:0010564 GO:0010565 GO:0010604 GO:0010605 GO:0010628 GO:0010638 GO:0010639 GO:0016020 GO:0016567 GO:0016740 GO:0019216 GO:0019217 GO:0019219 GO:0019222 GO:0019538 GO:0019787 GO:0031056 GO:0031057 GO:0031058 GO:0031323 GO:0031324 GO:0031325 GO:0031326 GO:0031327 GO:0031328 GO:0031399 GO:0031400 GO:0031401 GO:0031436 GO:0032268 GO:0032269 GO:0032270 GO:0032446 GO:0032991 GO:0033043 GO:0033044 GO:0033554 GO:0034641 GO:0035065 GO:0035066 GO:0035067 GO:0036211 GO:0042127 GO:0042304 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044464 GO:0045717 GO:0045787 GO:0045833 GO:0045893 GO:0045922 GO:0045935 GO:0045944 GO:0046483 GO:0046890 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0050789 GO:0050794 GO:0050896 GO:0051055 GO:0051128 GO:0051129 GO:0051130 GO:0051171 GO:0051172 GO:0051173 GO:0051246 GO:0051247 GO:0051248 GO:0051252 GO:0051254 GO:0051716 GO:0051726 GO:0060255 GO:0062012 GO:0062014 GO:0065007 GO:0070531 GO:0070647 GO:0071156 GO:0071158 GO:0071214 GO:0071478 GO:0071479 GO:0071480 GO:0071704 GO:0071944 GO:0080090 GO:0090068 GO:0090304 GO:0104004 GO:0140096 GO:1901360 GO:1901564 GO:1901983 GO:1901984 GO:1901985 GO:1902275 GO:1902494 GO:1902680 GO:1903506 GO:1903508 GO:1905268 GO:1905269 GO:1990234 GO:2000112 GO:2000756 GO:2000757 GO:2000758 GO:2001141 GO:2001251 GO:2001252
KEGG Pathways
Metabolic & Signaling
ko03440 map03440
Pfam Domains
Protein Families
BRCT BRCT_2 DYW_deaminase PPR zf-C3HC4_2 zf-HC5HC2H zf-RING_2

Protein Analysis

1088

Amino Acids

120.85

Weight (kDa)

9.0

Isoelectric Point (pI)

53.25

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-RING_6 PF14835 4 - 69 2.9e-06 zf-RING of BARD1-type protein
zf-C3HC4_3 PF13920 13 - 59 5.1e-07 Zinc finger, C3HC4 type (RING finger)
zf-C3HC4_2 PF13923 15 - 53 1.3e-10 Zinc finger, C3HC4 type (RING finger)
zf-RING_2 PF13639 15 - 54 7.3e-08 Ring finger domain
zf-C3HC4 PF00097 16 - 53 2.8e-08 Zinc finger, C3HC4 type (RING finger)
zf-RING_UBOX PF13445 16 - 51 3.7e-07 RING-type zinc-finger
zf-C3HC4_4 PF15227 16 - 53 9.6e-07 zinc finger of C3HC4-type, RING
zf-HC5HC2H PF13771 735 - 813 3.8e-09 PHD-like zinc-binding domain
BRCT PF00533 865 - 939 1.2e-08 BRCA1 C Terminus (BRCT) domain
BRCT_2 PF16589 976 - 1087 4.5e-08 BRCT domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 2148
AatII GACGTC 1 cut(s) 1614
Acc36I ACCTGC 1 cut(s) 862
Acc65I GGTACC 1 cut(s) 3143
AccB1I GGYRCC 1 cut(s) 3143
AccBSI CCGCTC 1 cut(s) 2632
AccI GTMKAC 2 cut(s) 554, 2899
AciI CCGC 3 cut(s) 175, 2632, 3167
AclWI GGATC 4 cut(s) 157, 332, 2574, 2590
AcoI YGGCCR 1 cut(s) 1224
AcsI RAATTY 7 cut(s) 643, 900, 982, 1670, 1995, 2573, 2648
AcuI CTGAAG 5 cut(s) 333, 471, 1062, 1197, 2496
AcyI GRCGYC 1 cut(s) 1611
AfaI GTAC 3 cut(s) 1432, 2970, 3145
AfiI CCNNNNNNNGG 7 cut(s) 387, 459, 501, 662, 1414, 2894, 3163
AflII CTTAAG 1 cut(s) 2942
AflIII ACRYGT 2 cut(s) 255, 1283
AhlI ACTAGT 2 cut(s) 2075, 2830
AjnI CCWGG 2 cut(s) 1140, 2512
AjuI GAANNNNNNNTTGG 2 cut(s) 2449, 2481
AloI GAACNNNNNNTCC 2 cut(s) 2121, 2153
Alw21I GWGCWC 2 cut(s) 839, 849
Alw26I GTCTC 3 cut(s) 1266, 1498, 1609
AlwI GGATC 4 cut(s) 157, 332, 2574, 2590
AlwNI CAGNNNCTG 2 cut(s) 2211, 2569
Ama87I CYCGRG 1 cut(s) 20
AoxI GGCC 4 cut(s) 503, 830, 1224, 2884
ApeKI GCWGC 6 cut(s) 304, 1015, 1200, 2165, 3008, 3239
ApoI RAATTY 7 cut(s) 643, 900, 982, 1670, 1995, 2573, 2648
ArsI GACNNNNNNTTYG 2 cut(s) 536, 568
Asp700I GAANNNNTTC 1 cut(s) 2136
Asp718I GGTACC 1 cut(s) 3143
AspLEI GCGC 3 cut(s) 16, 277, 2716
AspS9I GGNCC 4 cut(s) 975, 1138, 2184, 2885
AsuC2I CCSGG 1 cut(s) 519
AsuHPI GGTGA 4 cut(s) 600, 677, 959, 1712
AsuII TTCGAA 1 cut(s) 816
AsuNHI GCTAGC 1 cut(s) 1637
AvaI CYCGRG 1 cut(s) 20
AvaII GGWCC 3 cut(s) 975, 1138, 2184
BaeI ACNNNNGTAYC 2 cut(s) 2680, 2713
BalI TGGCCA 1 cut(s) 1226
BanI GGYRCC 1 cut(s) 3143
BanII GRGCYC 3 cut(s) 755, 849, 2227
BbsI GAAGAC 2 cut(s) 94, 741
Bbv12I GWGCWC 2 cut(s) 839, 849
BbvI GCAGC 6 cut(s) 316, 1002, 1187, 2152, 2995, 3251
BccI CCATC 7 cut(s) 290, 694, 1081, 2394, 2793, 2876, 2930
BceAI ACGGC 5 cut(s) 62, 518, 817, 1213, 2054
BciT130I CCWGG 2 cut(s) 1142, 2514
BciVI GTATCC 1 cut(s) 2778
BclI TGATCA 5 cut(s) 331, 667, 886, 2521, 3118
BcnI CCSGG 1 cut(s) 519
BcoDI GTCTC 3 cut(s) 1266, 1498, 1609
BcuI ACTAGT 2 cut(s) 2075, 2830
BfmI CTRYAG 3 cut(s) 1584, 2166, 2909
BfrI CTTAAG 1 cut(s) 2942
BfuAI ACCTGC 1 cut(s) 862
BfuI GTATCC 1 cut(s) 2778
BglI GCCNNNNNGGC 1 cut(s) 759
BglII AGATCT 1 cut(s) 395
BisI GCNGC 6 cut(s) 305, 1016, 1201, 2166, 3009, 3240
BlpI GCTNAGC 1 cut(s) 2271
BlsI GCNGC 6 cut(s) 306, 1017, 1202, 2167, 3010, 3241
Bme1390I CCNGG 3 cut(s) 519, 1142, 2514
Bme18I GGWCC 3 cut(s) 975, 1138, 2184
BmeT110I CYCGRG 1 cut(s) 20
BmgT120I GGNCC 4 cut(s) 975, 1138, 2184, 2885
BmiI GGNNCC 8 cut(s) 142, 471, 2185, 2226, 2844, 2922, 3145, 3199
BmrFI CCNGG 3 cut(s) 519, 1142, 2514
BmrI ACTGGG 1 cut(s) 767
BmsI GCATC 9 cut(s) 229, 625, 858, 1036, 1308, 2056, 2132, 2333, 3160
BmtI GCTAGC 1 cut(s) 1641
BmuI ACTGGG 1 cut(s) 767
BpiI GAAGAC 2 cut(s) 94, 741
BpmI CTGGAG 3 cut(s) 2685, 3033, 3216
Bpu10I CCTNAGC 1 cut(s) 843
Bpu1102I GCTNAGC 1 cut(s) 2271
Bpu14I TTCGAA 1 cut(s) 816
BpuEI CTTGAG 1 cut(s) 3128
BpuMI CCSGG 1 cut(s) 519
BsaBI GATNNNNATC 5 cut(s) 336, 400, 885, 1050, 1185
BsaHI GRCGYC 1 cut(s) 1611
BsaI GGTCTC 1 cut(s) 1498
BsaJI CCNNGG 2 cut(s) 1227, 2187
Bsc4I CCNNNNNNNGG 7 cut(s) 387, 459, 501, 662, 1414, 2894, 3163
Bse1I ACTGG 2 cut(s) 603, 762
Bse8I GATNNNNATC 5 cut(s) 336, 400, 885, 1050, 1185
BseBI CCWGG 2 cut(s) 1142, 2514
BseDI CCNNGG 2 cut(s) 1227, 2187
BseGI GGATG 7 cut(s) 244, 1299, 2585, 2804, 2887, 3127, 3175
BseJI GATNNNNATC 5 cut(s) 336, 400, 885, 1050, 1185
BseLI CCNNNNNNNGG 7 cut(s) 387, 459, 501, 662, 1414, 2894, 3163
BseMII CTCAG 8 cut(s) 368, 738, 834, 1332, 1551, 1755, 2262, 2780
BseNI ACTGG 2 cut(s) 603, 762
BseRI GAGGAG 3 cut(s) 186, 2071, 2855
BseXI GCAGC 6 cut(s) 316, 1002, 1187, 2152, 2995, 3251
BseYI CCCAGC 1 cut(s) 365
BsgI GTGCAG 2 cut(s) 788, 2197
BshFI GGCC 4 cut(s) 505, 832, 1226, 2886
BshNI GGYRCC 1 cut(s) 3143
BsiHKAI GWGCWC 2 cut(s) 839, 849
BsiHKCI CYCGRG 1 cut(s) 20
BsiSI CCGG 1 cut(s) 518
BslFI GGGAC 3 cut(s) 1552, 1565, 2702
BslI CCNNNNNNNGG 7 cut(s) 387, 459, 501, 662, 1414, 2894, 3163
BsmAI GTCTC 3 cut(s) 1266, 1498, 1609
BsmFI GGGAC 3 cut(s) 1552, 1565, 2702
BsmI GAATGC 1 cut(s) 1759
BsnI GGCC 4 cut(s) 505, 832, 1226, 2886
Bso31I GGTCTC 1 cut(s) 1498
BsoBI CYCGRG 1 cut(s) 20
Bsp119I TTCGAA 1 cut(s) 816
Bsp1286I GDGCHC 4 cut(s) 755, 839, 849, 2227
Bsp1720I GCTNAGC 1 cut(s) 2271
BspACI CCGC 3 cut(s) 175, 2632, 3167
BspANI GGCC 4 cut(s) 505, 832, 1226, 2886
BspCNI CTCAG 8 cut(s) 367, 737, 835, 1333, 1552, 1756, 2263, 2781
BspLI GGNNCC 8 cut(s) 142, 471, 2185, 2226, 2844, 2922, 3145, 3199
BspMAI CTGCAG 2 cut(s) 2170, 2913
BspMI ACCTGC 1 cut(s) 862
BspOI GCTAGC 1 cut(s) 1641
BspPI GGATC 4 cut(s) 157, 332, 2574, 2590
BspQI GCTCTTC 2 cut(s) 1964, 2486
BspT104I TTCGAA 1 cut(s) 816
BspT107I GGYRCC 1 cut(s) 3143
BspTI CTTAAG 1 cut(s) 2942
BspTNI GGTCTC 1 cut(s) 1498
BsrBI CCGCTC 1 cut(s) 2632
BsrI ACTGG 2 cut(s) 603, 762
BssECI CCNNGG 2 cut(s) 1227, 2187
BssNI GRCGYC 1 cut(s) 1611
BssT1I CCWWGG 2 cut(s) 1227, 2187
Bst2UI CCWGG 2 cut(s) 1142, 2514
Bst4CI ACNGT 8 cut(s) 382, 513, 740, 1217, 1579, 1892, 2674, 3022
Bst6I CTCTTC 4 cut(s) 1964, 2339, 2449, 2486
BstACI GRCGYC 1 cut(s) 1611
BstAFI CTTAAG 1 cut(s) 2942
BstAPI GCANNNNNTGC 1 cut(s) 3191
BstBI TTCGAA 1 cut(s) 816
BstF5I GGATG 7 cut(s) 244, 1299, 2585, 2804, 2887, 3127, 3175
BstHHI GCGC 3 cut(s) 16, 277, 2716
BstMAI GTCTC 3 cut(s) 1266, 1498, 1609
BstMWI GCNNNNNNNGC 5 cut(s) 281, 759, 1643, 3034, 3191
BstNI CCWGG 2 cut(s) 1142, 2514
BstNSI RCATGY 3 cut(s) 259, 1287, 2739
BstSCI CCNGG 3 cut(s) 517, 1140, 2512
BstSFI CTRYAG 3 cut(s) 1584, 2166, 2909
BstV1I GCAGC 6 cut(s) 316, 1002, 1187, 2152, 2995, 3251
BstV2I GAAGAC 2 cut(s) 94, 741
BstX2I RGATCY 4 cut(s) 162, 337, 395, 2566
BstXI CCANNNNNNTGG 1 cut(s) 2596
BstYI RGATCY 4 cut(s) 162, 337, 395, 2566
BsuI GTATCC 1 cut(s) 2778
BsuRI GGCC 4 cut(s) 505, 832, 1226, 2886
BtsCI GGATG 7 cut(s) 244, 1299, 2585, 2804, 2887, 3127, 3175
BtsI GCAGTG 3 cut(s) 957, 1342, 1396
BtsIMutI CAGTG 6 cut(s) 715, 755, 957, 1342, 1396, 2214
BveI ACCTGC 1 cut(s) 862
CaiI CAGNNNCTG 2 cut(s) 2211, 2569
CfoI GCGC 3 cut(s) 16, 277, 2716
Cfr13I GGNCC 4 cut(s) 975, 1138, 2184, 2885
CseI GACGC 1 cut(s) 832
CsiI ACCWGGT 2 cut(s) 1140, 2512
Csp6I GTAC 3 cut(s) 1431, 2969, 3144
CspCI CAANNNNNGTGG 2 cut(s) 1615, 1650
CviQI GTAC 3 cut(s) 1431, 2969, 3144
DraI TTTAAA 1 cut(s) 582
EaeI YGGCCR 1 cut(s) 1224
Eam1104I CTCTTC 4 cut(s) 1964, 2339, 2449, 2486
EarI CTCTTC 4 cut(s) 1964, 2339, 2449, 2486
EciI GGCGGA 1 cut(s) 3182
Ecl136II GAGCTC 1 cut(s) 847
Eco130I CCWWGG 2 cut(s) 1227, 2187
Eco24I GRGCYC 3 cut(s) 755, 849, 2227
Eco31I GGTCTC 1 cut(s) 1498
Eco32I GATATC 1 cut(s) 715
Eco47I GGWCC 3 cut(s) 975, 1138, 2184
Eco53kI GAGCTC 1 cut(s) 847
Eco57I CTGAAG 5 cut(s) 333, 471, 1062, 1197, 2496
Eco88I CYCGRG 1 cut(s) 20
EcoICRI GAGCTC 1 cut(s) 847
EcoRI GAATTC 1 cut(s) 900
EcoRII CCWGG 2 cut(s) 1140, 2512
EcoRV GATATC 1 cut(s) 715
EcoT14I CCWWGG 2 cut(s) 1227, 2187
EcoT38I GRGCYC 3 cut(s) 755, 849, 2227
ErhI CCWWGG 2 cut(s) 1227, 2187
FalI AAGNNNNNCTT 2 cut(s) 2936, 2968
FaqI GGGAC 3 cut(s) 1552, 1565, 2702
FauI CCCGC 1 cut(s) 2625
FauNDI CATATG 2 cut(s) 201, 1134
FbaI TGATCA 5 cut(s) 331, 667, 886, 2521, 3118
FblI GTMKAC 2 cut(s) 554, 2899
Fnu4HI GCNGC 6 cut(s) 305, 1016, 1201, 2166, 3009, 3240
FokI GGATG 7 cut(s) 251, 1286, 2572, 2811, 2894, 3114, 3182
FriOI GRGCYC 3 cut(s) 755, 849, 2227
Fsp4HI GCNGC 6 cut(s) 305, 1016, 1201, 2166, 3009, 3240
GlaI GCGC 3 cut(s) 15, 276, 2715
GluI GCNGC 6 cut(s) 305, 1016, 1201, 2166, 3009, 3240
GsaI CCCAGC 1 cut(s) 369
GsuI CTGGAG 3 cut(s) 2685, 3033, 3216
HaeIII GGCC 4 cut(s) 505, 832, 1226, 2886
HapII CCGG 1 cut(s) 518
HgaI GACGC 1 cut(s) 832
HhaI GCGC 3 cut(s) 16, 277, 2716
Hin1I GRCGYC 1 cut(s) 1611
Hin6I GCGC 3 cut(s) 14, 275, 2714
HinP1I GCGC 3 cut(s) 14, 275, 2714
HincII GTYRAC 4 cut(s) 653, 1353, 1931, 2863
HindII GTYRAC 4 cut(s) 653, 1353, 1931, 2863
HindIII AAGCTT 5 cut(s) 924, 1398, 2024, 2808, 2927
HpaI GTTAAC 1 cut(s) 1353
HpaII CCGG 1 cut(s) 518
HphI GGTGA 4 cut(s) 600, 677, 959, 1712
Hpy166II GTNNAC 8 cut(s) 555, 653, 950, 1353, 1931, 2140, 2863, 2900
Hpy8I GTNNAC 8 cut(s) 555, 653, 950, 1353, 1931, 2140, 2863, 2900
HpyCH4III ACNGT 8 cut(s) 382, 513, 740, 1217, 1579, 1892, 2674, 3022
HpyCH4IV ACGT 3 cut(s) 1611, 1825, 2232
HpyF10VI GCNNNNNNNGC 5 cut(s) 281, 759, 1643, 3034, 3191
HpySE526I ACGT 3 cut(s) 1611, 1825, 2232
Hsp92I GRCGYC 1 cut(s) 1611
HspAI GCGC 3 cut(s) 14, 275, 2714
KpnI GGTACC 1 cut(s) 3147
Ksp22I TGATCA 5 cut(s) 331, 667, 886, 2521, 3118
KspAI GTTAAC 1 cut(s) 1353
LguI GCTCTTC 2 cut(s) 1964, 2486
LmnI GCTCC 8 cut(s) 199, 842, 1762, 2230, 2321, 2732, 2842, 3197
Lsp1109I GCAGC 6 cut(s) 316, 1002, 1187, 2152, 2995, 3251
LweI GCATC 9 cut(s) 229, 625, 858, 1036, 1308, 2056, 2132, 2333, 3160
MabI ACCWGGT 2 cut(s) 1140, 2512
MaeII ACGT 3 cut(s) 1611, 1825, 2232
MbiI CCGCTC 1 cut(s) 2632
MfeI CAATTG 1 cut(s) 145
MflI RGATCY 4 cut(s) 162, 337, 395, 2566
MhlI GDGCHC 4 cut(s) 755, 839, 849, 2227
MlsI TGGCCA 1 cut(s) 1226
MluNI TGGCCA 1 cut(s) 1226
MmeI TCCRAC 2 cut(s) 473, 1784
Mox20I TGGCCA 1 cut(s) 1226
MroXI GAANNNNTTC 1 cut(s) 2136
MscI TGGCCA 1 cut(s) 1226
MslI CAYNNNNRTG 1 cut(s) 260
Msp20I TGGCCA 1 cut(s) 1226
MspA1I CMGCKG 1 cut(s) 175
MspCI CTTAAG 1 cut(s) 2942
MspI CCGG 1 cut(s) 518
MspR9I CCNGG 3 cut(s) 519, 1142, 2514
MunI CAATTG 1 cut(s) 145
Mva1269I GAATGC 1 cut(s) 1759
MvaI CCWGG 2 cut(s) 1142, 2514
MwoI GCNNNNNNNGC 5 cut(s) 281, 759, 1643, 3034, 3191
NciI CCSGG 1 cut(s) 519
NdeI CATATG 2 cut(s) 201, 1134
NheI GCTAGC 1 cut(s) 1637
NlaIV GGNNCC 8 cut(s) 142, 471, 2185, 2226, 2844, 2922, 3145, 3199
NmeAIII GCCGAG 1 cut(s) 1243
NmuCI GTSAC 3 cut(s) 626, 955, 1211
NspI RCATGY 3 cut(s) 259, 1287, 2739
NspV TTCGAA 1 cut(s) 816
PaeI GCATGC 1 cut(s) 2739
PaeR7I CTCGAG 1 cut(s) 20
PciI ACATGT 2 cut(s) 255, 1283
PciSI GCTCTTC 2 cut(s) 1964, 2486
PcsI WCGNNNNNNNCGW 1 cut(s) 830
PctI GAATGC 1 cut(s) 1759
PdmI GAANNNNTTC 1 cut(s) 2136
PfeI GAWTC 9 cut(s) 401, 422, 1918, 2114, 2260, 2405, 2471, 2766, 2874
PkrI GCNGC 6 cut(s) 306, 1017, 1202, 2167, 3010, 3241
PscI ACATGT 2 cut(s) 255, 1283
PsiI TTATAA 1 cut(s) 2148
Psp124BI GAGCTC 1 cut(s) 849
Psp6I CCWGG 2 cut(s) 1140, 2512
PspFI CCCAGC 1 cut(s) 365
PspGI CCWGG 2 cut(s) 1140, 2512
PspN4I GGNNCC 8 cut(s) 142, 471, 2185, 2226, 2844, 2922, 3145, 3199
PspPI GGNCC 4 cut(s) 975, 1138, 2184, 2885
PstI CTGCAG 2 cut(s) 2170, 2913
PstNI CAGNNNCTG 2 cut(s) 2211, 2569
PsuI RGATCY 4 cut(s) 162, 337, 395, 2566
RsaI GTAC 3 cut(s) 1432, 2970, 3145
RsaNI GTAC 3 cut(s) 1431, 2969, 3144
RseI CAYNNNNRTG 1 cut(s) 260
SacI GAGCTC 1 cut(s) 849
SapI GCTCTTC 2 cut(s) 1964, 2486
SatI GCNGC 6 cut(s) 305, 1016, 1201, 2166, 3009, 3240
Sau96I GGNCC 4 cut(s) 975, 1138, 2184, 2885
ScrFI CCNGG 3 cut(s) 519, 1142, 2514
SduI GDGCHC 4 cut(s) 755, 839, 849, 2227
SexAI ACCWGGT 2 cut(s) 1140, 2512
SfaNI GCATC 9 cut(s) 229, 625, 858, 1036, 1308, 2056, 2132, 2333, 3160
SfcI CTRYAG 3 cut(s) 1584, 2166, 2909
Sfr274I CTCGAG 1 cut(s) 20
SfuI TTCGAA 1 cut(s) 816
SinI GGWCC 3 cut(s) 975, 1138, 2184
SlaI CTCGAG 1 cut(s) 20
SmiMI CAYNNNNRTG 1 cut(s) 260
SmlI CTYRAG 3 cut(s) 20, 2942, 3107
SmoI CTYRAG 3 cut(s) 20, 2942, 3107
SpeI ACTAGT 2 cut(s) 2075, 2830
SphI GCATGC 1 cut(s) 2739
SsiI CCGC 3 cut(s) 175, 2632, 3167
SstI GAGCTC 1 cut(s) 849
StyD4I CCNGG 3 cut(s) 517, 1140, 2512
StyI CCWWGG 2 cut(s) 1227, 2187
TaaI ACNGT 8 cut(s) 382, 513, 740, 1217, 1579, 1892, 2674, 3022
TaiI ACGT 3 cut(s) 1614, 1828, 2235
TaqII GACCGA 1 cut(s) 2022
TfiI GAWTC 9 cut(s) 401, 422, 1918, 2114, 2260, 2405, 2471, 2766, 2874
TscAI CASTG 6 cut(s) 715, 762, 964, 1342, 1396, 2221
TseFI GTSAC 3 cut(s) 626, 955, 1211
TseI GCWGC 6 cut(s) 304, 1015, 1200, 2165, 3008, 3239
Tsp45I GTSAC 3 cut(s) 626, 955, 1211
TspGWI ACGGA 1 cut(s) 1448
TspRI CASTG 6 cut(s) 715, 762, 964, 1342, 1396, 2221
Vha464I CTTAAG 1 cut(s) 2942
VpaK11BI GGWCC 3 cut(s) 975, 1138, 2184
XapI RAATTY 7 cut(s) 643, 900, 982, 1670, 1995, 2573, 2648
XbaI TCTAGA 2 cut(s) 743, 986
XceI RCATGY 3 cut(s) 259, 1287, 2739
XhoI CTCGAG 1 cut(s) 20
XmiI GTMKAC 2 cut(s) 554, 2899
XmnI GAANNNNTTC 1 cut(s) 2136
ZraI GACGTC 1 cut(s) 1612
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.