Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr05

Orthogroup ID

OG0005162

Physical Location & Seq

Genomic Coordinates Reverse (-)

12406233 .. 12420808

Gene Structure 14576 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD05G1066200.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1500 bp

ATGGCGGAGGAGGCAATTCTAGGATACCTGGAGTCAAAGGAGGAAATATCAGATTCCGGTCAGTTTGCAGCTGAACGTGGGCTCGATCACAACGAGGTTGTCAACGTTATCAAGAGCCTCCATGGTTTCAGATATGTTGTCGCACAGGACATCAAAAGAGAGATGTGGATGCTTACTGATGAAGGAAGGGCATACACTGCTACCGGGTCACCGGAAGTGCAGCTGTTCCTGGCCATACCACCAGAGGGTATCCCCAAAGAAGAATTGCAGAAAAAGCTGGATCCATCAGTTTTCAAAATTGGAAGCGCCCAGGCTGCGAAGAGAAAATGGGTGGATATGGGCAAGCAACTAGTAACCCGAAGGATCCAACATGTGGAGGACGAAGTAAAAAATTTGCTTCTACAGATACAAGATGGGCAGGCCATCAACCAAGATGAAATTAAGGCTCTCAAAGCAAGAAAGCTTATTGTTGCACAAACATGGAAGGGCTACTTGGTGAAAAAGGGCCCCAATTATGCTCCAAAGAGAAAGAAAGTAGCTACAGATTTGACTCGAGAGAATCTTCAAAGGGGCGATTGGAAGGAACTGGAGTTCAAAGAGTATAACTTCAGCGCTAAAGGTCTGCCTATTGAAAGTGGTCATCTTCATCCGCTGCTCAAGGCAAGAACAGATTTCCATTGTATATTTTTAAAAGTAGTACGACATCAGTTGAAGGGTATTTTCAGGCAGATGGGGTTTGAGGAGATGCCAACAAACAATTATGTCGAGAGCAGCTTCTGGAACTTTGATGCGCTTTTCCAGCCACAACAACACCCTGCCCGTGATTCGCATGATACATTTTTCCTACAAGTTCCTTCAACCACCAATGAGCTGCCTGAAGATTACGTTGAGCGGGTGAAGCAGATTCATGAGTCTGGTGGTTATCAATCCCGAGGATATGGGTATGATTGGAAAAGAGAGGAAGCAGACAAAAATCTTCTGCGAACTCACACAACTGCTGTTTCCTCCAGGATGCTGTACGCACTAGCCCAGAAACCATTTGTGCCCAAAAAGTACTACTCTATTGATCGTGTGTTCAGAAATGAAGCAGTAGATCGAACACATCTTGCAGAGTTCCACCAGATAGAAGGTTTGGTATGTGATCGAGGGCTTGCTCTTGGTGACTTGTTAGGAGTACTCAAAGAATTCTTCTCACGTCTGGGCATGTCGAAACTGCGATTCAAGCCAGCTTATAATCCATATACCGAGCCTAGCATGGAGATTTTCAGTTATCATGAAGGCTTGGGGAAATGGGTAGAAATTGGAAATTCAGGCATGTTTAGACCTGAAATGCTGCTTCCTATGGGACTCCCGGAAGATGTTCGTGTTATTGCATGGGGCCTCTCCCTCGAAAGACCGACGATGATTCTCTATGGGATTGATAACATCAGAGATCTTTTCGGACACAAGGTCGATCTTGGTCTCATCAAGAGAAACCCAGTCTGTCGGCTTGGATTATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004812 GO:0004826 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0006082 GO:0006139 GO:0006399 GO:0006412 GO:0006418 GO:0006432 GO:0006518 GO:0006520 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009328 GO:0009987 GO:0010467 GO:0016070 GO:0016874 GO:0016875 GO:0019538 GO:0019752 GO:0032991 GO:0034641 GO:0034645 GO:0034660 GO:0043038 GO:0043039 GO:0043043 GO:0043170 GO:0043436 GO:0043603 GO:0043604 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044271 GO:0044281 GO:0044424 GO:0044444 GO:0044464 GO:0046483 GO:0071704 GO:0090304 GO:0140098 GO:0140101 GO:1901360 GO:1901564 GO:1901566 GO:1901576 GO:1902494

KEGG Pathways

Metabolic & Signaling

ko00970 map00970

Pfam Domains

Protein Families

tRNA-synt_2d

Protein Analysis

500

Amino Acids

57.42

Weight (kDa)

8.43

Isoelectric Point (pI)

39.56

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PheRS_DBD1	PF18552	3 - 53	2.8e-09	PheRS DNA binding domain 1
PheRS_DBD3	PF18553	70 - 124	7.3e-13	PheRS DNA binding domain 3
tRNA-synt_2d	PF01409	228 - 485	2.8e-86	tRNA synthetases class II core domain (F)

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0005162)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G39280 AT4G39280
fragaria_vesca	FvH4_2g08790 FvH4_2g08790 FvH4_3g28200 FvH4_3g28200
malus_domestica	MD05G1066200.v1.1 MD10G1076200.v1.1
prunus_persica	Prupe.8G096800_v2.0.a1 Prupe.8G096800_v2.0.a1 Prupe.8G096800_v2.0.a1
pyrus_communis	pycom10g05960
rosa_chinensis	RchiOBHm_Chr6g0264251 RchiOBHm_Chr7g0204471
rosa_laevigata	RLG00000003457 RLG00000014275
rosa_multiflora	Rmu_co8442849.1_g000001 Rmu_sc0010483.1_g000023
rosa_roxburghii	Rroxscaffold_176G00431660 Rroxscaffold_3G00253060
rosa_rugosa	Rorug06G0011500 Rorug07G0084400
rosa_samantha	Rh6AG130900 Rh6BG128000 Rh6CG126400 Rh6DG113500 Rh7AG213800 Rh7CG226600 Rh7DG221200
rosa_wichuraiana	Rw6G011440

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	1233, 1498
AccB7I	CCANNNNNTGG	1 cut(s)	373
AccBSI	CCGCTC	1 cut(s)	892
AciI	CCGC	3 cut(s)	5, 650, 892
AclI	AACGTT	1 cut(s)	105
AclWI	GGATC	4 cut(s)	275, 288, 358, 371
AcoI	YGGCCR	1 cut(s)	231
AcsI	RAATTY	3 cut(s)	391, 1184, 1306
AcuI	CTGAAG	2 cut(s)	592, 897
AfaI	GTAC	4 cut(s)	699, 1019, 1055, 1176
AfeI	AGCGCT	1 cut(s)	613
AfiI	CCNNNNNNNGG	2 cut(s)	245, 373
AflIII	ACRYGT	1 cut(s)	370
AgsI	TTSAA	7 cut(s)	295, 566, 595, 632, 712, 858, 1222
AhdI	GACNNNNNGTC	1 cut(s)	1448
AhlI	ACTAGT	1 cut(s)	349
AjiI	CACGTC	1 cut(s)	1196
AjnI	CCWGG	4 cut(s)	27, 228, 309, 1007
AjuI	GAANNNNNNNTTGG	2 cut(s)	476, 508
AloI	GAACNNNNNNTCC	2 cut(s)	575, 607
AluBI	AGCT	8 cut(s)	71, 223, 277, 463, 539, 774, 871, 1229
AluI	AGCT	8 cut(s)	71, 223, 277, 463, 539, 774, 871, 1229
Alw26I	GTCTC	1 cut(s)	1466
AlwI	GGATC	4 cut(s)	275, 288, 358, 371
AlwNI	CAGNNNCTG	1 cut(s)	777
Ama87I	CYCGRG	2 cut(s)	552, 930
Aor51HI	AGCGCT	1 cut(s)	613
AoxI	GGCC	4 cut(s)	231, 420, 505, 1378
ApaI	GGGCCC	1 cut(s)	509
ApeKI	GCWGC	7 cut(s)	68, 220, 314, 652, 771, 871, 1333
ApoI	RAATTY	3 cut(s)	391, 1184, 1306
Asp700I	GAANNNNTTC	1 cut(s)	1359
AspLEI	GCGC	3 cut(s)	308, 614, 793
AspS9I	GGNCC	3 cut(s)	505, 506, 1378
AsuC2I	CCSGG	2 cut(s)	205, 1352
AsuHPI	GGTGA	4 cut(s)	201, 508, 907, 1172
AvaI	CYCGRG	2 cut(s)	552, 930
BaeGI	GKGCMC	2 cut(s)	509, 1047
BalI	TGGCCA	1 cut(s)	233
BamHI	GGATCC	2 cut(s)	280, 363
BanII	GRGCYC	2 cut(s)	84, 509
BbvI	GCAGC	7 cut(s)	80, 232, 301, 639, 783, 858, 1320
BccI	CCATC	4 cut(s)	292, 407, 431, 724
BciT130I	CCWGG	4 cut(s)	29, 230, 311, 1009
BciVI	GTATCC	2 cut(s)	17, 260
BcnI	CCSGG	2 cut(s)	205, 1352
BcoDI	GTCTC	1 cut(s)	1466
BcuI	ACTAGT	1 cut(s)	349
BfaI	CTAG	4 cut(s)	20, 350, 1025, 1251
BfmI	CTRYAG	2 cut(s)	401, 540
BfoI	RGCGCY	2 cut(s)	309, 615
BfuI	GTATCC	2 cut(s)	17, 260
BglII	AGATCT	1 cut(s)	1432
BisI	GCNGC	7 cut(s)	69, 221, 315, 653, 772, 872, 1334
BlsI	GCNGC	7 cut(s)	70, 222, 316, 654, 773, 873, 1335
BmcAI	AGTACT	2 cut(s)	1055, 1176
Bme1390I	CCNGG	6 cut(s)	29, 205, 230, 311, 1009, 1352
BmeRI	GACNNNNNGTC	1 cut(s)	1448
BmeT110I	CYCGRG	2 cut(s)	552, 930
BmgBI	CACGTC	1 cut(s)	1196
BmgT120I	GGNCC	3 cut(s)	505, 506, 1378
BmiI	GGNNCC	5 cut(s)	282, 365, 507, 508, 1379
BmrFI	CCNGG	6 cut(s)	29, 205, 230, 311, 1009, 1352
BmrI	ACTGGG	1 cut(s)	1472
BmsI	GCATC	4 cut(s)	159, 735, 778, 1002
BmuI	ACTGGG	1 cut(s)	1472
BpmI	CTGGAG	3 cut(s)	50, 608, 991
BpuEI	CTTGAG	1 cut(s)	641
BpuMI	CCSGG	2 cut(s)	205, 1352
BsaI	GGTCTC	1 cut(s)	1466
BsaJI	CCNNGG	3 cut(s)	121, 309, 931
BsaWI	WCCGGW	2 cut(s)	56, 211
Bsc4I	CCNNNNNNNGG	2 cut(s)	245, 373
Bse1I	ACTGG	2 cut(s)	591, 1478
BseBI	CCWGG	4 cut(s)	29, 230, 311, 1009
BseDI	CCNNGG	3 cut(s)	121, 309, 931
BseGI	GGATG	3 cut(s)	174, 646, 1017
BseLI	CCNNNNNNNGG	2 cut(s)	245, 373
BseNI	ACTGG	2 cut(s)	591, 1478
BseRI	GAGGAG	2 cut(s)	23, 755
BseSI	GKGCMC	2 cut(s)	509, 1047
BseXI	GCAGC	7 cut(s)	80, 232, 301, 639, 783, 858, 1320
BsgI	GTGCAG	1 cut(s)	239
BshFI	GGCC	4 cut(s)	233, 422, 507, 1380
BsiHKCI	CYCGRG	2 cut(s)	552, 930
BsiSI	CCGG	4 cut(s)	57, 204, 212, 1352
BslFI	GGGAC	1 cut(s)	1359
BslI	CCNNNNNNNGG	2 cut(s)	245, 373
BsmAI	GTCTC	1 cut(s)	1466
BsmFI	GGGAC	1 cut(s)	1359
BsnI	GGCC	4 cut(s)	233, 422, 507, 1380
Bso31I	GGTCTC	1 cut(s)	1466
BsoBI	CYCGRG	2 cut(s)	552, 930
Bsp120I	GGGCCC	1 cut(s)	505
Bsp1286I	GDGCHC	3 cut(s)	84, 509, 1047
Bsp143I	GATC	8 cut(s)	85, 280, 363, 1066, 1093, 1141, 1432, 1453
Bsp19I	CCATGG	1 cut(s)	121
BspACI	CCGC	3 cut(s)	5, 650, 892
BspANI	GGCC	4 cut(s)	233, 422, 507, 1380
BspHI	TCATGA	2 cut(s)	907, 1273
BspLI	GGNNCC	5 cut(s)	282, 365, 507, 508, 1379
BspPI	GGATC	4 cut(s)	275, 288, 358, 371
BspTNI	GGTCTC	1 cut(s)	1466
BsrBI	CCGCTC	1 cut(s)	892
BsrI	ACTGG	2 cut(s)	591, 1478
BssECI	CCNNGG	3 cut(s)	121, 309, 931
BssMI	GATC	8 cut(s)	85, 280, 363, 1066, 1093, 1141, 1432, 1453
BssT1I	CCWWGG	1 cut(s)	121
Bst2UI	CCWGG	4 cut(s)	29, 230, 311, 1009
Bst6I	CTCTTC	1 cut(s)	314
BstAPI	GCANNNNNTGC	1 cut(s)	197
BstC8I	GCNNGC	4 cut(s)	344, 420, 1152, 1227
BstDSI	CCRYGG	1 cut(s)	121
BstEII	GGTNACC	1 cut(s)	207
BstF5I	GGATG	3 cut(s)	174, 646, 1017
BstH2I	RGCGCY	2 cut(s)	309, 615
BstHHI	GCGC	3 cut(s)	308, 614, 793
BstKTI	GATC	8 cut(s)	88, 283, 366, 1069, 1096, 1144, 1435, 1456
BstMAI	GTCTC	1 cut(s)	1466
BstMBI	GATC	8 cut(s)	85, 280, 363, 1066, 1093, 1141, 1432, 1453
BstMWI	GCNNNNNNNGC	8 cut(s)	11, 197, 274, 314, 452, 799, 898, 1222
BstNI	CCWGG	4 cut(s)	29, 230, 311, 1009
BstNSI	RCATGY	3 cut(s)	374, 1207, 1318
BstPI	GGTNACC	1 cut(s)	207
BstSCI	CCNGG	6 cut(s)	27, 203, 228, 309, 1007, 1350
BstSFI	CTRYAG	2 cut(s)	401, 540
BstSLI	GKGCMC	2 cut(s)	509, 1047
BstV1I	GCAGC	7 cut(s)	80, 232, 301, 639, 783, 858, 1320
BstX2I	RGATCY	3 cut(s)	280, 363, 1432
BstYI	RGATCY	3 cut(s)	280, 363, 1432
BsuI	GTATCC	2 cut(s)	17, 260
BsuRI	GGCC	4 cut(s)	233, 422, 507, 1380
BtgI	CCRYGG	1 cut(s)	121
BtrI	CACGTC	1 cut(s)	1196
BtsCI	GGATG	3 cut(s)	174, 646, 1017
BtsI	GCAGTG	1 cut(s)	195
BtsIMutI	CAGTG	1 cut(s)	195
Cac8I	GCNNGC	4 cut(s)	344, 420, 1152, 1227
CaiI	CAGNNNCTG	1 cut(s)	777
CciI	TCATGA	2 cut(s)	907, 1273
CfoI	GCGC	3 cut(s)	308, 614, 793
Cfr13I	GGNCC	3 cut(s)	505, 506, 1378
Csp6I	GTAC	4 cut(s)	698, 1018, 1054, 1175
CviQI	GTAC	4 cut(s)	698, 1018, 1054, 1175
DpnI	GATC	8 cut(s)	87, 282, 365, 1068, 1095, 1143, 1434, 1455
DpnII	GATC	8 cut(s)	85, 280, 363, 1066, 1093, 1141, 1432, 1453
DraI	TTTAAA	1 cut(s)	690
DriI	GACNNNNNGTC	1 cut(s)	1448
EaeI	YGGCCR	1 cut(s)	231
Eam1104I	CTCTTC	1 cut(s)	314
Eam1105I	GACNNNNNGTC	1 cut(s)	1448
EarI	CTCTTC	1 cut(s)	314
EciI	GGCGGA	1 cut(s)	20
Eco130I	CCWWGG	1 cut(s)	121
Eco24I	GRGCYC	2 cut(s)	84, 509
Eco31I	GGTCTC	1 cut(s)	1466
Eco47III	AGCGCT	1 cut(s)	613
Eco57I	CTGAAG	2 cut(s)	592, 897
Eco88I	CYCGRG	2 cut(s)	552, 930
Eco91I	GGTNACC	1 cut(s)	207
EcoO109I	RGGNCCY	3 cut(s)	505, 506, 1378
EcoO65I	GGTNACC	1 cut(s)	207
EcoRI	GAATTC	1 cut(s)	1184
EcoRII	CCWGG	4 cut(s)	27, 228, 309, 1007
EcoT14I	CCWWGG	1 cut(s)	121
EcoT38I	GRGCYC	2 cut(s)	84, 509
ErhI	CCWWGG	1 cut(s)	121
FalI	AAGNNNNNCTT	2 cut(s)	476, 508
FaqI	GGGAC	1 cut(s)	1359
FauI	CCCGC	1 cut(s)	885
Fnu4HI	GCNGC	7 cut(s)	69, 221, 315, 653, 772, 872, 1334
FokI	GGATG	3 cut(s)	181, 633, 1024
FriOI	GRGCYC	2 cut(s)	84, 509
Fsp4HI	GCNGC	7 cut(s)	69, 221, 315, 653, 772, 872, 1334
FspBI	CTAG	4 cut(s)	20, 350, 1025, 1251
GlaI	GCGC	3 cut(s)	307, 613, 792
GluI	GCNGC	7 cut(s)	69, 221, 315, 653, 772, 872, 1334
GsuI	CTGGAG	3 cut(s)	50, 608, 991
HaeII	RGCGCY	2 cut(s)	309, 615
HaeIII	GGCC	4 cut(s)	233, 422, 507, 1380
HapII	CCGG	4 cut(s)	57, 204, 212, 1352
HhaI	GCGC	3 cut(s)	308, 614, 793
Hin6I	GCGC	3 cut(s)	306, 612, 791
HinP1I	GCGC	3 cut(s)	306, 612, 791
HincII	GTYRAC	1 cut(s)	103
HindII	GTYRAC	1 cut(s)	103
HindIII	AAGCTT	1 cut(s)	461
HpaII	CCGG	4 cut(s)	57, 204, 212, 1352
HphI	GGTGA	4 cut(s)	201, 508, 907, 1172
Hpy166II	GTNNAC	1 cut(s)	103
Hpy188I	TCNGA	5 cut(s)	52, 131, 1079, 1430, 1442
Hpy188III	TCNNGA	8 cut(s)	112, 554, 766, 778, 908, 930, 1274, 1468
Hpy8I	GTNNAC	1 cut(s)	103
Hpy99I	CGWCG	1 cut(s)	1402
HpyAV	CCTTC	9 cut(s)	176, 180, 354, 478, 574, 706, 864, 1121, 1271
HpyCH4IV	ACGT	4 cut(s)	76, 105, 885, 1195
HpyCH4V	TGCA	6 cut(s)	68, 220, 268, 473, 1109, 1373
HpyF10VI	GCNNNNNNNGC	8 cut(s)	11, 197, 274, 314, 452, 799, 898, 1222
HpySE526I	ACGT	4 cut(s)	76, 105, 885, 1195
HspAI	GCGC	3 cut(s)	306, 612, 791
Kzo9I	GATC	8 cut(s)	85, 280, 363, 1066, 1093, 1141, 1432, 1453
LmnI	GCTCC	1 cut(s)	523
Lsp1109I	GCAGC	7 cut(s)	80, 232, 301, 639, 783, 858, 1320
LweI	GCATC	4 cut(s)	159, 735, 778, 1002
MaeI	CTAG	4 cut(s)	20, 350, 1025, 1251
MaeII	ACGT	4 cut(s)	76, 105, 885, 1195
MaeIII	GTNAC	3 cut(s)	207, 352, 1160
MalI	GATC	8 cut(s)	87, 282, 365, 1068, 1095, 1143, 1434, 1455
MbiI	CCGCTC	1 cut(s)	892
MboI	GATC	8 cut(s)	85, 280, 363, 1066, 1093, 1141, 1432, 1453
MboII	GAAGA	8 cut(s)	272, 331, 554, 635, 890, 968, 1180, 1367
MflI	RGATCY	3 cut(s)	280, 363, 1432
MhlI	GDGCHC	3 cut(s)	84, 509, 1047
MlsI	TGGCCA	1 cut(s)	233
MluNI	TGGCCA	1 cut(s)	233
MlyI	GAGTC	4 cut(s)	41, 544, 920, 1341
MmeI	TCCRAC	1 cut(s)	391
Mox20I	TGGCCA	1 cut(s)	233
MroXI	GAANNNNTTC	1 cut(s)	1359
MscI	TGGCCA	1 cut(s)	233
MseI	TTAA	2 cut(s)	441, 689
MslI	CAYNNNNRTG	1 cut(s)	478
Msp20I	TGGCCA	1 cut(s)	233
MspA1I	CMGCKG	3 cut(s)	71, 223, 652
MspI	CCGG	4 cut(s)	57, 204, 212, 1352
MspR9I	CCNGG	6 cut(s)	29, 205, 230, 311, 1009, 1352
MvaI	CCWGG	4 cut(s)	29, 230, 311, 1009
MwoI	GCNNNNNNNGC	8 cut(s)	11, 197, 274, 314, 452, 799, 898, 1222
NciI	CCSGG	2 cut(s)	205, 1352
NcoI	CCATGG	1 cut(s)	121
NdeII	GATC	8 cut(s)	85, 280, 363, 1066, 1093, 1141, 1432, 1453
NlaIV	GGNNCC	5 cut(s)	282, 365, 507, 508, 1379
NmuCI	GTSAC	2 cut(s)	207, 1160
NspI	RCATGY	3 cut(s)	374, 1207, 1318
PaeR7I	CTCGAG	1 cut(s)	552
PagI	TCATGA	2 cut(s)	907, 1273
PciI	ACATGT	1 cut(s)	370
PcsI	WCGNNNNNNNCGW	1 cut(s)	90
PdmI	GAANNNNTTC	1 cut(s)	1359
PfeI	GAWTC	6 cut(s)	53, 559, 824, 904, 1218, 1405
PflMI	CCANNNNNTGG	1 cut(s)	373
PfoI	TCCNGGA	2 cut(s)	1007, 1350
PkrI	GCNGC	7 cut(s)	70, 222, 316, 654, 773, 873, 1335
PleI	GAGTC	4 cut(s)	40, 544, 919, 1341
PpsI	GAGTC	4 cut(s)	40, 544, 919, 1341
PscI	ACATGT	1 cut(s)	370
PsiI	TTATAA	2 cut(s)	1233, 1498
Psp1406I	AACGTT	1 cut(s)	105
Psp6I	CCWGG	4 cut(s)	27, 228, 309, 1007
PspEI	GGTNACC	1 cut(s)	207
PspGI	CCWGG	4 cut(s)	27, 228, 309, 1007
PspN4I	GGNNCC	5 cut(s)	282, 365, 507, 508, 1379
PspOMI	GGGCCC	1 cut(s)	505
PspPI	GGNCC	3 cut(s)	505, 506, 1378
PstNI	CAGNNNCTG	1 cut(s)	777
PsuI	RGATCY	3 cut(s)	280, 363, 1432
PvuII	CAGCTG	2 cut(s)	71, 223
RsaI	GTAC	4 cut(s)	699, 1019, 1055, 1176
RsaNI	GTAC	4 cut(s)	698, 1018, 1054, 1175
RseI	CAYNNNNRTG	1 cut(s)	478
SaqAI	TTAA	2 cut(s)	441, 689
SatI	GCNGC	7 cut(s)	69, 221, 315, 653, 772, 872, 1334
Sau3AI	GATC	8 cut(s)	85, 280, 363, 1066, 1093, 1141, 1432, 1453
Sau96I	GGNCC	3 cut(s)	505, 506, 1378
ScaI	AGTACT	2 cut(s)	1055, 1176
SchI	GAGTC	4 cut(s)	41, 544, 920, 1341
ScrFI	CCNGG	6 cut(s)	29, 205, 230, 311, 1009, 1352
SduI	GDGCHC	3 cut(s)	84, 509, 1047
SfaNI	GCATC	4 cut(s)	159, 735, 778, 1002
SfcI	CTRYAG	2 cut(s)	401, 540
Sfr274I	CTCGAG	1 cut(s)	552
SlaI	CTCGAG	1 cut(s)	552
SmiMI	CAYNNNNRTG	1 cut(s)	478
SmlI	CTYRAG	2 cut(s)	552, 656
SmoI	CTYRAG	2 cut(s)	552, 656
SpeI	ACTAGT	1 cut(s)	349
SsiI	CCGC	3 cut(s)	5, 650, 892
SspMI	CTAG	4 cut(s)	20, 350, 1025, 1251
StyD4I	CCNGG	6 cut(s)	27, 203, 228, 309, 1007, 1350
StyI	CCWWGG	1 cut(s)	121
TaiI	ACGT	4 cut(s)	79, 108, 888, 1198
TaqI	TCGA	8 cut(s)	84, 553, 765, 1096, 1144, 1208, 1389, 1452
TaqII	GACCGA	1 cut(s)	1411
TatI	WGTACW	2 cut(s)	1053, 1174
TfiI	GAWTC	6 cut(s)	53, 559, 824, 904, 1218, 1405
Tru1I	TTAA	2 cut(s)	441, 689
Tru9I	TTAA	2 cut(s)	441, 689
TscAI	CASTG	1 cut(s)	202
TseFI	GTSAC	2 cut(s)	207, 1160
TseI	GCWGC	7 cut(s)	68, 220, 314, 652, 771, 871, 1333
Tsp45I	GTSAC	2 cut(s)	207, 1160
TspDTI	ATGAA	6 cut(s)	195, 450, 635, 896, 1098, 1290
TspRI	CASTG	1 cut(s)	202
Van91I	CCANNNNNTGG	1 cut(s)	373
XapI	RAATTY	3 cut(s)	391, 1184, 1306
XceI	RCATGY	3 cut(s)	374, 1207, 1318
XhoI	CTCGAG	1 cut(s)	552
XmnI	GAANNNNTTC	1 cut(s)	1359
XspI	CTAG	4 cut(s)	20, 350, 1025, 1251
ZrmI	AGTACT	2 cut(s)	1055, 1176

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.