MD05G1072400.v1.1

PetM family of cytochrome b6f complex subunit 7

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0005452
Physical Location & Seq
Reverse (-)
15265191 .. 15265577
387 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1072400.v1.1.491

Sequence Viewer

Length: 387 bp
ATGGCAACAGCCTCAGTCACTGTCTCTCCAGCAATGGTTGCTGCCACCACAGTGGCCAAATCAAGGAGGATGACTTCATCAGCAAACAATGTGACATACATAACAGGACTCAATGCCTTCTCTGGACTCAGAGCTCAGAGCAGTGTGGCCTCCCTTGGCCTTCCACAGAGCACTGAGCCGTCCTTTGCTAGGGTTGTGAGCTCATTGAGAGCCCCATCAAGGGGCAGTGGTGGTGGTGGTGGTGCACTGTCTTCCACCTGCAATGCTGTTGGTGAGATTTTTAAGATTGCTGCCATCCTCAATGGACTCACTCTCGTCGGAGTTGCAGTCGGATTCGTGCTTCTTCGGATTGAAGCAGCGGTTGAGGAGGCGGCTGAGGCTGAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PetM

Protein Analysis

129

Amino Acids

12.73

Weight (kDa)

9.18

Isoelectric Point (pI)

54.95

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PetM PF08041 91 - 118 1.3e-10 PetM family of cytochrome b6f complex subunit 7
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 266
Acc36I ACCTGC 1 cut(s) 266
AciI CCGC 2 cut(s) 359, 371
AcoI YGGCCR 1 cut(s) 54
AfiI CCNNNNNNNGG 5 cut(s) 63, 189, 219, 220, 221
AgsI TTSAA 1 cut(s) 353
AleI CACNNNNGTG 1 cut(s) 50
AluBI AGCT 2 cut(s) 134, 201
AluI AGCT 2 cut(s) 134, 201
Alw21I GWGCWC 4 cut(s) 136, 173, 203, 247
Alw26I GTCTC 1 cut(s) 28
Alw44I GTGCAC 1 cut(s) 243
AlwNI CAGNNNCTG 1 cut(s) 20
AoxI GGCC 3 cut(s) 54, 147, 157
ApaLI GTGCAC 1 cut(s) 243
ApeKI GCWGC 3 cut(s) 41, 290, 356
AsuHPI GGTGA 1 cut(s) 284
BaeGI GKGCMC 1 cut(s) 247
BalI TGGCCA 1 cut(s) 56
BanII GRGCYC 3 cut(s) 136, 203, 214
BbsI GAAGAC 1 cut(s) 243
Bbv12I GWGCWC 4 cut(s) 136, 173, 203, 247
BbvCI CCTCAGC 1 cut(s) 375
BbvI GCAGC 3 cut(s) 28, 277, 368
BccI CCATC 2 cut(s) 223, 302
BceAI ACGGC 1 cut(s) 163
BcoDI GTCTC 1 cut(s) 28
BfaI CTAG 1 cut(s) 189
BfuAI ACCTGC 1 cut(s) 266
BisI GCNGC 4 cut(s) 42, 291, 357, 372
BlsI GCNGC 4 cut(s) 43, 292, 358, 373
BpiI GAAGAC 1 cut(s) 243
BpmI CTGGAG 1 cut(s) 12
Bpu10I CCTNAGC 1 cut(s) 375
BsaJI CCNNGG 1 cut(s) 154
BsaXI ACNNNNNCTCC 4 cut(s) 10, 40, 312, 342
Bsc4I CCNNNNNNNGG 5 cut(s) 63, 189, 219, 220, 221
Bse3DI GCAATG 2 cut(s) 39, 268
BseDI CCNNGG 1 cut(s) 154
BseGI GGATG 2 cut(s) 75, 294
BseLI CCNNNNNNNGG 5 cut(s) 63, 189, 219, 220, 221
BseMI GCAATG 2 cut(s) 39, 268
BseMII CTCAG 6 cut(s) 27, 142, 149, 165, 366, 372
BseRI GAGGAG 1 cut(s) 380
BseSI GKGCMC 1 cut(s) 247
BseXI GCAGC 3 cut(s) 28, 277, 368
BshFI GGCC 3 cut(s) 56, 149, 159
BsiHKAI GWGCWC 4 cut(s) 136, 173, 203, 247
BslI CCNNNNNNNGG 5 cut(s) 63, 189, 219, 220, 221
BsmAI GTCTC 1 cut(s) 28
BsnI GGCC 3 cut(s) 56, 149, 159
Bsp1286I GDGCHC 5 cut(s) 136, 173, 203, 214, 247
BspACI CCGC 2 cut(s) 359, 371
BspANI GGCC 3 cut(s) 56, 149, 159
BspCNI CTCAG 6 cut(s) 26, 141, 148, 166, 367, 373
BspMI ACCTGC 1 cut(s) 266
BsrDI GCAATG 2 cut(s) 39, 268
BssECI CCNNGG 1 cut(s) 154
BssT1I CCWWGG 1 cut(s) 154
Bst4CI ACNGT 3 cut(s) 22, 52, 249
BstAPI GCANNNNNTGC 1 cut(s) 38
BstDEI CTNAG 6 cut(s) 13, 128, 135, 174, 375, 381
BstENI CCTNNNNNAGG 1 cut(s) 187
BstF5I GGATG 2 cut(s) 75, 294
BstMAI GTCTC 1 cut(s) 28
BstMWI GCNNNNNNNGC 2 cut(s) 38, 377
BstSLI GKGCMC 1 cut(s) 247
BstV1I GCAGC 3 cut(s) 28, 277, 368
BstV2I GAAGAC 1 cut(s) 243
BstXI CCANNNNNNTGG 1 cut(s) 52
BsuRI GGCC 3 cut(s) 56, 149, 159
BtsCI GGATG 2 cut(s) 75, 294
BtsI GCAGTG 2 cut(s) 148, 232
BtsIMutI CAGTG 6 cut(s) 18, 57, 148, 171, 232, 245
BveI ACCTGC 1 cut(s) 266
CaiI CAGNNNCTG 1 cut(s) 20
DdeI CTNAG 6 cut(s) 13, 128, 135, 174, 375, 381
EaeI YGGCCR 1 cut(s) 54
Ecl136II GAGCTC 2 cut(s) 134, 201
Eco130I CCWWGG 1 cut(s) 154
Eco24I GRGCYC 3 cut(s) 136, 203, 214
Eco53kI GAGCTC 2 cut(s) 134, 201
EcoICRI GAGCTC 2 cut(s) 134, 201
EcoNI CCTNNNNNAGG 1 cut(s) 187
EcoT14I CCWWGG 1 cut(s) 154
EcoT38I GRGCYC 3 cut(s) 136, 203, 214
ErhI CCWWGG 1 cut(s) 154
FaiI YATR 2 cut(s) 97, 101
Fnu4HI GCNGC 4 cut(s) 42, 291, 357, 372
FokI GGATG 2 cut(s) 82, 281
FriOI GRGCYC 3 cut(s) 136, 203, 214
Fsp4HI GCNGC 4 cut(s) 42, 291, 357, 372
FspBI CTAG 1 cut(s) 189
GluI GCNGC 4 cut(s) 42, 291, 357, 372
GsuI CTGGAG 1 cut(s) 12
HaeIII GGCC 3 cut(s) 56, 149, 159
HinfI GANTC 4 cut(s) 108, 126, 306, 333
HphI GGTGA 1 cut(s) 284
Hpy166II GTNNAC 1 cut(s) 245
Hpy188I TCNGA 5 cut(s) 131, 138, 320, 332, 348
Hpy188III TCNNGA 1 cut(s) 123
Hpy8I GTNNAC 1 cut(s) 245
Hpy99I CGWCG 1 cut(s) 320
HpyAV CCTTC 2 cut(s) 127, 170
HpyCH4III ACNGT 3 cut(s) 22, 52, 249
HpyCH4V TGCA 3 cut(s) 245, 261, 326
HpyF10VI GCNNNNNNNGC 2 cut(s) 38, 377
HpyF3I CTNAG 6 cut(s) 13, 128, 135, 174, 375, 381
LpnPI CCDG 4 cut(s) 42, 90, 108, 271
Lsp1109I GCAGC 3 cut(s) 28, 277, 368
MaeI CTAG 1 cut(s) 189
MaeIII GTNAC 2 cut(s) 16, 91
MboII GAAGA 2 cut(s) 243, 335
MhlI GDGCHC 5 cut(s) 136, 173, 203, 214, 247
MlsI TGGCCA 1 cut(s) 56
MluNI TGGCCA 1 cut(s) 56
MlyI GAGTC 3 cut(s) 102, 120, 300
MmeI TCCRAC 2 cut(s) 298, 310
MnlI CCTC 7 cut(s) 22, 60, 160, 308, 358, 361, 370
Mox20I TGGCCA 1 cut(s) 56
MscI TGGCCA 1 cut(s) 56
MseI TTAA 1 cut(s) 282
MslI CAYNNNNRTG 1 cut(s) 50
Msp20I TGGCCA 1 cut(s) 56
MspA1I CMGCKG 1 cut(s) 359
MwoI GCNNNNNNNGC 2 cut(s) 38, 377
NmuCI GTSAC 2 cut(s) 16, 91
OliI CACNNNNGTG 1 cut(s) 50
PaqCI CACCTGC 1 cut(s) 266
PfeI GAWTC 1 cut(s) 333
PkrI GCNGC 4 cut(s) 43, 292, 358, 373
PleI GAGTC 3 cut(s) 102, 120, 300
PpsI GAGTC 3 cut(s) 102, 120, 300
Psp124BI GAGCTC 2 cut(s) 136, 203
PstNI CAGNNNCTG 1 cut(s) 20
RseI CAYNNNNRTG 1 cut(s) 50
SacI GAGCTC 2 cut(s) 136, 203
SaqAI TTAA 1 cut(s) 282
SatI GCNGC 4 cut(s) 42, 291, 357, 372
SchI GAGTC 3 cut(s) 102, 120, 300
SduI GDGCHC 5 cut(s) 136, 173, 203, 214, 247
SetI ASST 3 cut(s) 136, 203, 260
SmiMI CAYNNNNRTG 1 cut(s) 50
SsiI CCGC 2 cut(s) 359, 371
SspMI CTAG 1 cut(s) 189
SstI GAGCTC 2 cut(s) 136, 203
StyI CCWWGG 1 cut(s) 154
TaaI ACNGT 3 cut(s) 22, 52, 249
TauI GCSGC 1 cut(s) 374
TfiI GAWTC 1 cut(s) 333
Tru1I TTAA 1 cut(s) 282
Tru9I TTAA 1 cut(s) 282
TscAI CASTG 6 cut(s) 25, 57, 148, 178, 232, 252
TseFI GTSAC 2 cut(s) 16, 91
TseI GCWGC 3 cut(s) 41, 290, 356
Tsp45I GTSAC 2 cut(s) 16, 91
TspDTI ATGAA 1 cut(s) 66
TspRI CASTG 6 cut(s) 25, 57, 148, 178, 232, 252
VneI GTGCAC 1 cut(s) 243
XagI CCTNNNNNAGG 1 cut(s) 187
XspI CTAG 1 cut(s) 189
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.