pycom10g06920

PetM family of cytochrome b6f complex subunit 7

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr10
OG0005452
Physical Location & Seq
Reverse (-)
8072766 .. 8073122
357 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom10g06920.1

Sequence Viewer

Length: 357 bp
ATGGTTGCTGCCACCACAGTGGCCAAATCAAGGAGGAGGATGTCATCAGCAAACAACGTGACTTACCTAACAGGACTCAATGCCTTCTCTGGACTCAAAGCTCAGAGCAGTGTGGCCTCCCTTGGCCTCCCACGGACCACTGAGCACTCCTTTGCTAGGGTTGTGAGCTCCTTGAGAGCCCCATCAAAGGGTAACGGCAGTGGTGGTGGTGGTGCACTGTCGTCCACCTGCAATGCTGTTGATGAGATTTTCAAGATTGCTGCCATCCTCAATGGACTCACTCGCGTCGGAGTTGCGGTTGGATTCGTGCTTCTTCGGATCGAAGCCGCGGTTGAGGAGGCAGCTGAGGCTGAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PetM

Protein Analysis

119

Amino Acids

12.01

Weight (kDa)

9.85

Isoelectric Point (pI)

45.07

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PetM PF08041 82 - 108 5.4e-09 PetM family of cytochrome b6f complex subunit 7
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 236
Acc36I ACCTGC 1 cut(s) 236
AccII CGCG 2 cut(s) 285, 329
AciI CCGC 3 cut(s) 296, 327, 329
AclWI GGATC 1 cut(s) 326
AcoI YGGCCR 1 cut(s) 21
AfiI CCNNNNNNNGG 4 cut(s) 30, 156, 187, 188
AgsI TTSAA 1 cut(s) 253
AleI CACNNNNGTG 1 cut(s) 17
AluBI AGCT 3 cut(s) 101, 168, 344
AluI AGCT 3 cut(s) 101, 168, 344
Alw21I GWGCWC 3 cut(s) 147, 170, 217
Alw44I GTGCAC 1 cut(s) 213
AlwI GGATC 1 cut(s) 326
AoxI GGCC 3 cut(s) 21, 114, 124
ApaLI GTGCAC 1 cut(s) 213
ApeKI GCWGC 3 cut(s) 8, 260, 341
AspS9I GGNCC 1 cut(s) 135
AvaII GGWCC 1 cut(s) 135
BaeGI GKGCMC 1 cut(s) 217
BalI TGGCCA 1 cut(s) 23
BanII GRGCYC 2 cut(s) 170, 181
Bbv12I GWGCWC 3 cut(s) 147, 170, 217
BbvCI CCTCAGC 1 cut(s) 345
BbvI GCAGC 2 cut(s) 247, 353
BccI CCATC 2 cut(s) 190, 272
BceAI ACGGC 1 cut(s) 211
BfaI CTAG 1 cut(s) 156
BfuAI ACCTGC 1 cut(s) 236
BisI GCNGC 4 cut(s) 9, 261, 327, 342
BlsI GCNGC 4 cut(s) 10, 262, 328, 343
Bme18I GGWCC 1 cut(s) 135
BmgT120I GGNCC 1 cut(s) 135
Bpu10I CCTNAGC 1 cut(s) 345
BpuEI CTTGAG 1 cut(s) 193
BsaJI CCNNGG 3 cut(s) 121, 131, 327
BsaXI ACNNNNNCTCC 2 cut(s) 282, 312
Bsc4I CCNNNNNNNGG 4 cut(s) 30, 156, 187, 188
Bse3DI GCAATG 1 cut(s) 238
BseDI CCNNGG 3 cut(s) 121, 131, 327
BseGI GGATG 2 cut(s) 45, 264
BseLI CCNNNNNNNGG 4 cut(s) 30, 156, 187, 188
BseMI GCAATG 1 cut(s) 238
BseMII CTCAG 4 cut(s) 116, 132, 336, 342
BseRI GAGGAG 2 cut(s) 49, 350
BseSI GKGCMC 1 cut(s) 217
BseXI GCAGC 2 cut(s) 247, 353
Bsh1236I CGCG 2 cut(s) 285, 329
BshFI GGCC 3 cut(s) 23, 116, 126
BsiHKAI GWGCWC 3 cut(s) 147, 170, 217
BslI CCNNNNNNNGG 4 cut(s) 30, 156, 187, 188
BsnI GGCC 3 cut(s) 23, 116, 126
Bsp1286I GDGCHC 4 cut(s) 147, 170, 181, 217
Bsp143I GATC 1 cut(s) 318
BspACI CCGC 3 cut(s) 296, 327, 329
BspANI GGCC 3 cut(s) 23, 116, 126
BspCNI CTCAG 4 cut(s) 115, 133, 337, 343
BspFNI CGCG 2 cut(s) 285, 329
BspMI ACCTGC 1 cut(s) 236
BspPI GGATC 1 cut(s) 326
BsrDI GCAATG 1 cut(s) 238
BssECI CCNNGG 3 cut(s) 121, 131, 327
BssMI GATC 1 cut(s) 318
BssT1I CCWWGG 1 cut(s) 121
Bst4CI ACNGT 2 cut(s) 19, 219
BstDEI CTNAG 4 cut(s) 102, 141, 345, 351
BstDSI CCRYGG 2 cut(s) 131, 327
BstENI CCTNNNNNAGG 1 cut(s) 154
BstF5I GGATG 2 cut(s) 45, 264
BstFNI CGCG 2 cut(s) 285, 329
BstKTI GATC 1 cut(s) 321
BstMBI GATC 1 cut(s) 318
BstMWI GCNNNNNNNGC 1 cut(s) 347
BstSLI GKGCMC 1 cut(s) 217
BstUI CGCG 2 cut(s) 285, 329
BstV1I GCAGC 2 cut(s) 247, 353
BstXI CCANNNNNNTGG 1 cut(s) 19
BsuRI GGCC 3 cut(s) 23, 116, 126
BtgI CCRYGG 2 cut(s) 131, 327
BtsCI GGATG 2 cut(s) 45, 264
BtsI GCAGTG 2 cut(s) 115, 205
BtsIMutI CAGTG 5 cut(s) 24, 115, 138, 205, 215
BveI ACCTGC 1 cut(s) 236
Cfr13I GGNCC 1 cut(s) 135
Cfr42I CCGCGG 1 cut(s) 330
CseI GACGC 1 cut(s) 274
CviJI RGCY 9 cut(s) 23, 101, 116, 126, 168, 179, 326, 344, 350
CviKI_1 RGCY 9 cut(s) 23, 101, 116, 126, 168, 179, 326, 344, 350
DdeI CTNAG 4 cut(s) 102, 141, 345, 351
DpnI GATC 1 cut(s) 320
DpnII GATC 1 cut(s) 318
EaeI YGGCCR 1 cut(s) 21
Ecl136II GAGCTC 1 cut(s) 168
Eco130I CCWWGG 1 cut(s) 121
Eco24I GRGCYC 2 cut(s) 170, 181
Eco47I GGWCC 1 cut(s) 135
Eco53kI GAGCTC 1 cut(s) 168
EcoICRI GAGCTC 1 cut(s) 168
EcoNI CCTNNNNNAGG 1 cut(s) 154
EcoT14I CCWWGG 1 cut(s) 121
EcoT38I GRGCYC 2 cut(s) 170, 181
ErhI CCWWGG 1 cut(s) 121
Fnu4HI GCNGC 4 cut(s) 9, 261, 327, 342
FokI GGATG 2 cut(s) 52, 251
FriOI GRGCYC 2 cut(s) 170, 181
Fsp4HI GCNGC 4 cut(s) 9, 261, 327, 342
FspBI CTAG 1 cut(s) 156
GluI GCNGC 4 cut(s) 9, 261, 327, 342
HaeIII GGCC 3 cut(s) 23, 116, 126
HgaI GACGC 1 cut(s) 274
HinfI GANTC 4 cut(s) 75, 93, 276, 303
Hpy166II GTNNAC 2 cut(s) 215, 225
Hpy188I TCNGA 3 cut(s) 105, 290, 318
Hpy188III TCNNGA 2 cut(s) 90, 253
Hpy8I GTNNAC 2 cut(s) 215, 225
Hpy99I CGWCG 1 cut(s) 290
HpyAV CCTTC 1 cut(s) 94
HpyCH4III ACNGT 2 cut(s) 19, 219
HpyCH4IV ACGT 1 cut(s) 57
HpyCH4V TGCA 2 cut(s) 215, 231
HpyF10VI GCNNNNNNNGC 1 cut(s) 347
HpyF3I CTNAG 4 cut(s) 102, 141, 345, 351
HpySE526I ACGT 1 cut(s) 57
KspI CCGCGG 1 cut(s) 330
Kzo9I GATC 1 cut(s) 318
LmnI GCTCC 1 cut(s) 173
LpnPI CCDG 3 cut(s) 57, 75, 241
Lsp1109I GCAGC 2 cut(s) 247, 353
MaeI CTAG 1 cut(s) 156
MaeII ACGT 1 cut(s) 57
MaeIII GTNAC 2 cut(s) 58, 191
MalI GATC 1 cut(s) 320
MboI GATC 1 cut(s) 318
MboII GAAGA 1 cut(s) 305
MhlI GDGCHC 4 cut(s) 147, 170, 181, 217
MlsI TGGCCA 1 cut(s) 23
MluNI TGGCCA 1 cut(s) 23
MlyI GAGTC 3 cut(s) 69, 87, 270
MmeI TCCRAC 2 cut(s) 268, 280
MnlI CCTC 8 cut(s) 27, 30, 127, 137, 278, 328, 331, 340
Mox20I TGGCCA 1 cut(s) 23
MscI TGGCCA 1 cut(s) 23
MslI CAYNNNNRTG 1 cut(s) 17
Msp20I TGGCCA 1 cut(s) 23
MspA1I CMGCKG 2 cut(s) 329, 344
MvnI CGCG 2 cut(s) 285, 329
MwoI GCNNNNNNNGC 1 cut(s) 347
NdeII GATC 1 cut(s) 318
NmuCI GTSAC 1 cut(s) 58
OliI CACNNNNGTG 1 cut(s) 17
PaqCI CACCTGC 1 cut(s) 236
PfeI GAWTC 1 cut(s) 303
PkrI GCNGC 4 cut(s) 10, 262, 328, 343
PleI GAGTC 3 cut(s) 69, 87, 270
PpsI GAGTC 3 cut(s) 69, 87, 270
Psp124BI GAGCTC 1 cut(s) 170
PspPI GGNCC 1 cut(s) 135
PvuII CAGCTG 1 cut(s) 344
RseI CAYNNNNRTG 1 cut(s) 17
SacI GAGCTC 1 cut(s) 170
SacII CCGCGG 1 cut(s) 330
SatI GCNGC 4 cut(s) 9, 261, 327, 342
Sau3AI GATC 1 cut(s) 318
Sau96I GGNCC 1 cut(s) 135
SchI GAGTC 3 cut(s) 69, 87, 270
SduI GDGCHC 4 cut(s) 147, 170, 181, 217
SetI ASST 6 cut(s) 60, 69, 103, 170, 230, 346
Sfr303I CCGCGG 1 cut(s) 330
SgrBI CCGCGG 1 cut(s) 330
SinI GGWCC 1 cut(s) 135
SmiMI CAYNNNNRTG 1 cut(s) 17
SmlI CTYRAG 1 cut(s) 172
SmoI CTYRAG 1 cut(s) 172
SsiI CCGC 3 cut(s) 296, 327, 329
SspMI CTAG 1 cut(s) 156
SstI GAGCTC 1 cut(s) 170
StyI CCWWGG 1 cut(s) 121
TaaI ACNGT 2 cut(s) 19, 219
TaiI ACGT 1 cut(s) 60
TaqI TCGA 1 cut(s) 321
TauI GCSGC 1 cut(s) 329
TfiI GAWTC 1 cut(s) 303
TscAI CASTG 5 cut(s) 24, 115, 145, 205, 222
TseFI GTSAC 1 cut(s) 58
TseI GCWGC 3 cut(s) 8, 260, 341
Tsp45I GTSAC 1 cut(s) 58
TspGWI ACGGA 1 cut(s) 148
TspRI CASTG 5 cut(s) 24, 115, 145, 205, 222
VneI GTGCAC 1 cut(s) 213
VpaK11BI GGWCC 1 cut(s) 135
XagI CCTNNNNNAGG 1 cut(s) 154
XspI CTAG 1 cut(s) 156
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.