pycom05g06460

PetM family of cytochrome b6f complex subunit 7

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr5
OG0005452
Physical Location & Seq
Reverse (-)
8882156 .. 8882915
760 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom05g06460.2

Sequence Viewer

Length: 573 bp
ATGTTCTCTCTCATTAGAAACCAAAGGCTCATCCAATCTGCTCGTCCACCAACCATCCCTCTCTCACCTTATCCATTCAACATCTCTCATCCCATGCCATACAAAACTCAGCCCATTTGGTTTCCCTTCATCTCTTTACTTGTAGAAACCACAAACCCCATAACAAAAAAGAAAGTTATAGCAATGGCAACAGCCTCAGCCACTGTCTCTCCAGCAATGGTTGCTGCCACCACAGTGGCCAAATCAAGGAGGATGAATTCATCAGCAAACAATGTGACATACATAACAGGACTCAATGCCTTCTCTGGACTCAAAGCTCAGAGCAGTGTGGCCTCCCTTGGCCTTCCACAGAGCACTGAGCAATCCTTTGCTAGGGTTGTGAGCTCATTGAGAGCCCCATCAAGGGGCAATGGCAGTGGTGGTGGTGGTGCACTGTCTTCCACCTGCAATGCTGTTGGTGAGATTTTTAAGATTGCTGCCATCCTCAATGGACTCACTCTCGTCGGAGTTGCGGTCGGATTCGTGCTTCTTCGGATCGAAGCAGCGGTTGAGGAGGCGGCTGAGGCTGAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PetM

Protein Analysis

191

Amino Acids

19.89

Weight (kDa)

10.16

Isoelectric Point (pI)

52.58

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 452
Acc36I ACCTGC 1 cut(s) 452
AciI CCGC 3 cut(s) 512, 545, 557
AclWI GGATC 1 cut(s) 542
AcoI YGGCCR 1 cut(s) 237
AcsI RAATTY 1 cut(s) 256
AfiI CCNNNNNNNGG 5 cut(s) 246, 372, 402, 403, 404
AgsI TTSAA 1 cut(s) 79
AleI CACNNNNGTG 1 cut(s) 233
AluBI AGCT 2 cut(s) 317, 384
AluI AGCT 2 cut(s) 317, 384
Alw21I GWGCWC 3 cut(s) 356, 386, 433
Alw26I GTCTC 1 cut(s) 211
Alw44I GTGCAC 1 cut(s) 429
AlwI GGATC 1 cut(s) 542
AlwNI CAGNNNCTG 1 cut(s) 203
AoxI GGCC 3 cut(s) 237, 330, 340
ApaLI GTGCAC 1 cut(s) 429
ApeKI GCWGC 3 cut(s) 224, 476, 542
ApoI RAATTY 1 cut(s) 256
AsuHPI GGTGA 2 cut(s) 57, 470
BaeGI GKGCMC 1 cut(s) 433
BalI TGGCCA 1 cut(s) 239
BanII GRGCYC 2 cut(s) 386, 397
BbsI GAAGAC 1 cut(s) 429
Bbv12I GWGCWC 3 cut(s) 356, 386, 433
BbvCI CCTCAGC 2 cut(s) 196, 561
BbvI GCAGC 3 cut(s) 211, 463, 554
BccI CCATC 3 cut(s) 62, 406, 488
BcoDI GTCTC 1 cut(s) 211
BfaI CTAG 1 cut(s) 372
BfuAI ACCTGC 1 cut(s) 452
BisI GCNGC 4 cut(s) 225, 477, 543, 558
BlsI GCNGC 4 cut(s) 226, 478, 544, 559
BpiI GAAGAC 1 cut(s) 429
BpmI CTGGAG 1 cut(s) 195
Bpu10I CCTNAGC 2 cut(s) 196, 561
BsaJI CCNNGG 1 cut(s) 337
BsaXI ACNNNNNCTCC 4 cut(s) 193, 223, 498, 528
Bsc4I CCNNNNNNNGG 5 cut(s) 246, 372, 402, 403, 404
Bse3DI GCAATG 4 cut(s) 189, 222, 415, 454
BseDI CCNNGG 1 cut(s) 337
BseGI GGATG 5 cut(s) 30, 54, 88, 258, 480
BseLI CCNNNNNNNGG 5 cut(s) 246, 372, 402, 403, 404
BseMI GCAATG 4 cut(s) 189, 222, 415, 454
BseMII CTCAG 6 cut(s) 122, 210, 332, 348, 552, 558
BseRI GAGGAG 1 cut(s) 566
BseSI GKGCMC 1 cut(s) 433
BseXI GCAGC 3 cut(s) 211, 463, 554
Bsh1285I CGRYCG 1 cut(s) 516
BshFI GGCC 3 cut(s) 239, 332, 342
BsiEI CGRYCG 1 cut(s) 516
BsiHKAI GWGCWC 3 cut(s) 356, 386, 433
BslI CCNNNNNNNGG 5 cut(s) 246, 372, 402, 403, 404
BsmAI GTCTC 1 cut(s) 211
BsnI GGCC 3 cut(s) 239, 332, 342
Bsp1286I GDGCHC 4 cut(s) 356, 386, 397, 433
Bsp143I GATC 1 cut(s) 534
BspACI CCGC 3 cut(s) 512, 545, 557
BspANI GGCC 3 cut(s) 239, 332, 342
BspCNI CTCAG 6 cut(s) 121, 209, 331, 349, 553, 559
BspMI ACCTGC 1 cut(s) 452
BspPI GGATC 1 cut(s) 542
BsrDI GCAATG 4 cut(s) 189, 222, 415, 454
BssECI CCNNGG 1 cut(s) 337
BssMI GATC 1 cut(s) 534
BssT1I CCWWGG 1 cut(s) 337
Bst4CI ACNGT 3 cut(s) 205, 235, 435
BstAPI GCANNNNNTGC 1 cut(s) 221
BstDEI CTNAG 6 cut(s) 108, 196, 318, 357, 561, 567
BstENI CCTNNNNNAGG 1 cut(s) 370
BstF5I GGATG 5 cut(s) 30, 54, 88, 258, 480
BstKTI GATC 1 cut(s) 537
BstMAI GTCTC 1 cut(s) 211
BstMBI GATC 1 cut(s) 534
BstMCI CGRYCG 1 cut(s) 516
BstMWI GCNNNNNNNGC 2 cut(s) 221, 563
BstSLI GKGCMC 1 cut(s) 433
BstV1I GCAGC 3 cut(s) 211, 463, 554
BstV2I GAAGAC 1 cut(s) 429
BstXI CCANNNNNNTGG 1 cut(s) 235
BsuRI GGCC 3 cut(s) 239, 332, 342
BtsCI GGATG 5 cut(s) 30, 54, 88, 258, 480
BtsI GCAGTG 2 cut(s) 331, 421
BtsIMutI CAGTG 6 cut(s) 201, 240, 331, 354, 421, 431
BveI ACCTGC 1 cut(s) 452
CaiI CAGNNNCTG 1 cut(s) 203
CspCI CAANNNNNGTGG 2 cut(s) 397, 432
CviAII CATG 1 cut(s) 94
DdeI CTNAG 6 cut(s) 108, 196, 318, 357, 561, 567
DpnI GATC 1 cut(s) 536
DpnII GATC 1 cut(s) 534
EaeI YGGCCR 1 cut(s) 237
Ecl136II GAGCTC 1 cut(s) 384
Eco130I CCWWGG 1 cut(s) 337
Eco24I GRGCYC 2 cut(s) 386, 397
Eco53kI GAGCTC 1 cut(s) 384
EcoICRI GAGCTC 1 cut(s) 384
EcoNI CCTNNNNNAGG 1 cut(s) 370
EcoRI GAATTC 1 cut(s) 256
EcoT14I CCWWGG 1 cut(s) 337
EcoT38I GRGCYC 2 cut(s) 386, 397
ErhI CCWWGG 1 cut(s) 337
FaeI CATG 1 cut(s) 97
FaiI YATR 6 cut(s) 95, 100, 161, 179, 280, 284
FatI CATG 1 cut(s) 93
Fnu4HI GCNGC 4 cut(s) 225, 477, 543, 558
FokI GGATG 5 cut(s) 17, 41, 75, 265, 467
FriOI GRGCYC 2 cut(s) 386, 397
Fsp4HI GCNGC 4 cut(s) 225, 477, 543, 558
FspBI CTAG 1 cut(s) 372
GluI GCNGC 4 cut(s) 225, 477, 543, 558
GsuI CTGGAG 1 cut(s) 195
HaeIII GGCC 3 cut(s) 239, 332, 342
Hin1II CATG 1 cut(s) 97
HinfI GANTC 4 cut(s) 291, 309, 492, 519
HphI GGTGA 2 cut(s) 57, 470
Hpy166II GTNNAC 2 cut(s) 47, 431
Hpy188I TCNGA 4 cut(s) 321, 506, 518, 534
Hpy188III TCNNGA 1 cut(s) 306
Hpy8I GTNNAC 2 cut(s) 47, 431
Hpy99I CGWCG 1 cut(s) 506
HpyAV CCTTC 3 cut(s) 136, 310, 353
HpyCH4III ACNGT 3 cut(s) 205, 235, 435
HpyCH4V TGCA 2 cut(s) 431, 447
HpyF10VI GCNNNNNNNGC 2 cut(s) 221, 563
HpyF3I CTNAG 6 cut(s) 108, 196, 318, 357, 561, 567
Hsp92II CATG 1 cut(s) 97
Kzo9I GATC 1 cut(s) 534
LpnPI CCDG 4 cut(s) 225, 273, 291, 457
Lsp1109I GCAGC 3 cut(s) 211, 463, 554
MaeI CTAG 1 cut(s) 372
MaeIII GTNAC 1 cut(s) 274
MalI GATC 1 cut(s) 536
MboI GATC 1 cut(s) 534
MboII GAAGA 2 cut(s) 429, 521
MhlI GDGCHC 4 cut(s) 356, 386, 397, 433
MlsI TGGCCA 1 cut(s) 239
MluCI AATT 1 cut(s) 256
MluNI TGGCCA 1 cut(s) 239
MlyI GAGTC 3 cut(s) 285, 303, 486
MmeI TCCRAC 2 cut(s) 484, 496
MnlI CCTC 8 cut(s) 69, 205, 243, 343, 494, 544, 547, 556
Mox20I TGGCCA 1 cut(s) 239
MscI TGGCCA 1 cut(s) 239
MseI TTAA 1 cut(s) 468
MslI CAYNNNNRTG 1 cut(s) 233
Msp20I TGGCCA 1 cut(s) 239
MspA1I CMGCKG 1 cut(s) 545
MwoI GCNNNNNNNGC 2 cut(s) 221, 563
NdeII GATC 1 cut(s) 534
NlaIII CATG 1 cut(s) 97
NmuCI GTSAC 1 cut(s) 274
OliI CACNNNNGTG 1 cut(s) 233
PaqCI CACCTGC 1 cut(s) 452
PfeI GAWTC 1 cut(s) 519
PkrI GCNGC 4 cut(s) 226, 478, 544, 559
PleI GAGTC 3 cut(s) 285, 303, 486
PpsI GAGTC 3 cut(s) 285, 303, 486
Psp124BI GAGCTC 1 cut(s) 386
PstNI CAGNNNCTG 1 cut(s) 203
RseI CAYNNNNRTG 1 cut(s) 233
SacI GAGCTC 1 cut(s) 386
SaqAI TTAA 1 cut(s) 468
SatI GCNGC 4 cut(s) 225, 477, 543, 558
Sau3AI GATC 1 cut(s) 534
SchI GAGTC 3 cut(s) 285, 303, 486
SduI GDGCHC 4 cut(s) 356, 386, 397, 433
SetI ASST 4 cut(s) 70, 319, 386, 446
SmiMI CAYNNNNRTG 1 cut(s) 233
Sse9I AATT 1 cut(s) 256
SsiI CCGC 3 cut(s) 512, 545, 557
SspMI CTAG 1 cut(s) 372
SstI GAGCTC 1 cut(s) 386
StyI CCWWGG 1 cut(s) 337
TaaI ACNGT 3 cut(s) 205, 235, 435
TaqI TCGA 1 cut(s) 537
TasI AATT 1 cut(s) 256
TauI GCSGC 1 cut(s) 560
TfiI GAWTC 1 cut(s) 519
Tru1I TTAA 1 cut(s) 468
Tru9I TTAA 1 cut(s) 468
TscAI CASTG 6 cut(s) 208, 240, 331, 361, 421, 438
TseFI GTSAC 1 cut(s) 274
TseI GCWGC 3 cut(s) 224, 476, 542
Tsp45I GTSAC 1 cut(s) 274
TspDTI ATGAA 3 cut(s) 118, 249, 269
TspRI CASTG 6 cut(s) 208, 240, 331, 361, 421, 438
VneI GTGCAC 1 cut(s) 429
XagI CCTNNNNNAGG 1 cut(s) 370
XapI RAATTY 1 cut(s) 256
XspI CTAG 1 cut(s) 372
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.