MD10G1086700.v1.1

PetM family of cytochrome b6f complex subunit 7

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0005452
Physical Location & Seq
Reverse (-)
12849087 .. 12850081
995 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1086700.v1.1.491

Sequence Viewer

Length: 360 bp
ATGGTTGCTGCCACCACAGTGGCCAAATCAAGGAGGAGGATGTCATCAGCAAACAACGTGACTTACCTAACAGGACTCAATGCCTTTTCTGGACTCAAAGCTCAGAGCAGTGTGGCCTCCCTTGGCCTCCCACGGACTACTGAGCAGTCCTTTGCTAGGGTTGTGAGCTCCTTGAGATCCCCATCAAAGGGCAACGGAAGTGGTGGTGGTGGTGGTGCACTGTCGTCCACCTGCAATGCTGTTGGTGAGATTTTCAAGATTGCTGCCATCCTCAATGGACTCACTCTCGTCGGAGTTGCGGTTGGATTCGTGCTTCTTCGCATCGAAGCCGCGGTTGAGGAGGCAGCTGAGGCTGAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PetM

Protein Analysis

120

Amino Acids

11.97

Weight (kDa)

9.85

Isoelectric Point (pI)

57.43

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PetM PF08041 82 - 109 1.1e-10 PetM family of cytochrome b6f complex subunit 7
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 239
Acc36I ACCTGC 1 cut(s) 239
AccII CGCG 1 cut(s) 332
AciI CCGC 3 cut(s) 299, 330, 332
AclWI GGATC 1 cut(s) 171
AcoI YGGCCR 1 cut(s) 21
AfiI CCNNNNNNNGG 4 cut(s) 30, 156, 187, 188
AgsI TTSAA 1 cut(s) 256
AleI CACNNNNGTG 1 cut(s) 17
AluBI AGCT 3 cut(s) 101, 168, 347
AluI AGCT 3 cut(s) 101, 168, 347
Alw21I GWGCWC 2 cut(s) 170, 220
Alw44I GTGCAC 1 cut(s) 216
AlwI GGATC 1 cut(s) 171
AoxI GGCC 3 cut(s) 21, 114, 124
ApaLI GTGCAC 1 cut(s) 216
ApeKI GCWGC 3 cut(s) 8, 263, 344
AsuHPI GGTGA 1 cut(s) 257
BaeGI GKGCMC 1 cut(s) 220
BalI TGGCCA 1 cut(s) 23
BanII GRGCYC 1 cut(s) 170
Bbv12I GWGCWC 2 cut(s) 170, 220
BbvCI CCTCAGC 1 cut(s) 348
BbvI GCAGC 2 cut(s) 250, 356
BccI CCATC 2 cut(s) 190, 275
BfaI CTAG 1 cut(s) 156
BfuAI ACCTGC 1 cut(s) 239
BisI GCNGC 4 cut(s) 9, 264, 330, 345
BlsI GCNGC 4 cut(s) 10, 265, 331, 346
BmsI GCATC 1 cut(s) 330
Bpu10I CCTNAGC 1 cut(s) 348
BpuEI CTTGAG 1 cut(s) 193
BsaBI GATNNNNATC 1 cut(s) 181
BsaJI CCNNGG 3 cut(s) 121, 131, 330
BsaXI ACNNNNNCTCC 2 cut(s) 285, 315
Bsc4I CCNNNNNNNGG 4 cut(s) 30, 156, 187, 188
Bse3DI GCAATG 1 cut(s) 241
Bse8I GATNNNNATC 1 cut(s) 181
BseDI CCNNGG 3 cut(s) 121, 131, 330
BseGI GGATG 2 cut(s) 45, 267
BseJI GATNNNNATC 1 cut(s) 181
BseLI CCNNNNNNNGG 4 cut(s) 30, 156, 187, 188
BseMI GCAATG 1 cut(s) 241
BseMII CTCAG 4 cut(s) 116, 132, 339, 345
BseRI GAGGAG 2 cut(s) 49, 353
BseSI GKGCMC 1 cut(s) 220
BseXI GCAGC 2 cut(s) 250, 356
Bsh1236I CGCG 1 cut(s) 332
BshFI GGCC 3 cut(s) 23, 116, 126
BsiHKAI GWGCWC 2 cut(s) 170, 220
BslI CCNNNNNNNGG 4 cut(s) 30, 156, 187, 188
BsnI GGCC 3 cut(s) 23, 116, 126
Bsp1286I GDGCHC 2 cut(s) 170, 220
Bsp143I GATC 1 cut(s) 176
BspACI CCGC 3 cut(s) 299, 330, 332
BspANI GGCC 3 cut(s) 23, 116, 126
BspCNI CTCAG 4 cut(s) 115, 133, 340, 346
BspFNI CGCG 1 cut(s) 332
BspMI ACCTGC 1 cut(s) 239
BspPI GGATC 1 cut(s) 171
BsrDI GCAATG 1 cut(s) 241
BssECI CCNNGG 3 cut(s) 121, 131, 330
BssMI GATC 1 cut(s) 176
BssT1I CCWWGG 1 cut(s) 121
Bst4CI ACNGT 2 cut(s) 19, 222
BstDEI CTNAG 4 cut(s) 102, 141, 348, 354
BstDSI CCRYGG 2 cut(s) 131, 330
BstENI CCTNNNNNAGG 1 cut(s) 154
BstF5I GGATG 2 cut(s) 45, 267
BstFNI CGCG 1 cut(s) 332
BstKTI GATC 1 cut(s) 179
BstMBI GATC 1 cut(s) 176
BstMWI GCNNNNNNNGC 1 cut(s) 350
BstSLI GKGCMC 1 cut(s) 220
BstUI CGCG 1 cut(s) 332
BstV1I GCAGC 2 cut(s) 250, 356
BstX2I RGATCY 1 cut(s) 176
BstXI CCANNNNNNTGG 1 cut(s) 19
BstYI RGATCY 1 cut(s) 176
BsuRI GGCC 3 cut(s) 23, 116, 126
BtgI CCRYGG 2 cut(s) 131, 330
BtsCI GGATG 2 cut(s) 45, 267
BtsI GCAGTG 1 cut(s) 115
BtsIMutI CAGTG 3 cut(s) 24, 115, 218
BveI ACCTGC 1 cut(s) 239
Cfr42I CCGCGG 1 cut(s) 333
CspCI CAANNNNNGTGG 2 cut(s) 181, 216
CviJI RGCY 8 cut(s) 23, 101, 116, 126, 168, 329, 347, 353
CviKI_1 RGCY 8 cut(s) 23, 101, 116, 126, 168, 329, 347, 353
DdeI CTNAG 4 cut(s) 102, 141, 348, 354
DpnI GATC 1 cut(s) 178
DpnII GATC 1 cut(s) 176
EaeI YGGCCR 1 cut(s) 21
Ecl136II GAGCTC 1 cut(s) 168
Eco130I CCWWGG 1 cut(s) 121
Eco24I GRGCYC 1 cut(s) 170
Eco53kI GAGCTC 1 cut(s) 168
EcoICRI GAGCTC 1 cut(s) 168
EcoNI CCTNNNNNAGG 1 cut(s) 154
EcoT14I CCWWGG 1 cut(s) 121
EcoT38I GRGCYC 1 cut(s) 170
ErhI CCWWGG 1 cut(s) 121
Fnu4HI GCNGC 4 cut(s) 9, 264, 330, 345
FokI GGATG 2 cut(s) 52, 254
FriOI GRGCYC 1 cut(s) 170
Fsp4HI GCNGC 4 cut(s) 9, 264, 330, 345
FspBI CTAG 1 cut(s) 156
GluI GCNGC 4 cut(s) 9, 264, 330, 345
HaeIII GGCC 3 cut(s) 23, 116, 126
HinfI GANTC 4 cut(s) 75, 93, 279, 306
HphI GGTGA 1 cut(s) 257
Hpy166II GTNNAC 2 cut(s) 218, 228
Hpy188I TCNGA 2 cut(s) 105, 293
Hpy188III TCNNGA 2 cut(s) 90, 256
Hpy8I GTNNAC 2 cut(s) 218, 228
Hpy99I CGWCG 1 cut(s) 293
HpyCH4III ACNGT 2 cut(s) 19, 222
HpyCH4IV ACGT 1 cut(s) 57
HpyCH4V TGCA 2 cut(s) 218, 234
HpyF10VI GCNNNNNNNGC 1 cut(s) 350
HpyF3I CTNAG 4 cut(s) 102, 141, 348, 354
HpySE526I ACGT 1 cut(s) 57
KspI CCGCGG 1 cut(s) 333
Kzo9I GATC 1 cut(s) 176
LmnI GCTCC 1 cut(s) 173
LpnPI CCDG 3 cut(s) 57, 75, 244
Lsp1109I GCAGC 2 cut(s) 250, 356
LweI GCATC 1 cut(s) 330
MaeI CTAG 1 cut(s) 156
MaeII ACGT 1 cut(s) 57
MaeIII GTNAC 1 cut(s) 58
MalI GATC 1 cut(s) 178
MboI GATC 1 cut(s) 176
MboII GAAGA 1 cut(s) 308
MflI RGATCY 1 cut(s) 176
MhlI GDGCHC 2 cut(s) 170, 220
MlsI TGGCCA 1 cut(s) 23
MluNI TGGCCA 1 cut(s) 23
MlyI GAGTC 3 cut(s) 69, 87, 273
MmeI TCCRAC 2 cut(s) 271, 283
MnlI CCTC 8 cut(s) 27, 30, 127, 137, 281, 331, 334, 343
Mox20I TGGCCA 1 cut(s) 23
MscI TGGCCA 1 cut(s) 23
MslI CAYNNNNRTG 1 cut(s) 17
Msp20I TGGCCA 1 cut(s) 23
MspA1I CMGCKG 2 cut(s) 332, 347
MvnI CGCG 1 cut(s) 332
MwoI GCNNNNNNNGC 1 cut(s) 350
NdeII GATC 1 cut(s) 176
NmuCI GTSAC 1 cut(s) 58
OliI CACNNNNGTG 1 cut(s) 17
PaqCI CACCTGC 1 cut(s) 239
PfeI GAWTC 1 cut(s) 306
PkrI GCNGC 4 cut(s) 10, 265, 331, 346
PleI GAGTC 3 cut(s) 69, 87, 273
PpsI GAGTC 3 cut(s) 69, 87, 273
Psp124BI GAGCTC 1 cut(s) 170
PsuI RGATCY 1 cut(s) 176
PvuII CAGCTG 1 cut(s) 347
RseI CAYNNNNRTG 1 cut(s) 17
SacI GAGCTC 1 cut(s) 170
SacII CCGCGG 1 cut(s) 333
SatI GCNGC 4 cut(s) 9, 264, 330, 345
Sau3AI GATC 1 cut(s) 176
SchI GAGTC 3 cut(s) 69, 87, 273
SduI GDGCHC 2 cut(s) 170, 220
SetI ASST 6 cut(s) 60, 69, 103, 170, 233, 349
SfaNI GCATC 1 cut(s) 330
Sfr303I CCGCGG 1 cut(s) 333
SgrBI CCGCGG 1 cut(s) 333
SmiMI CAYNNNNRTG 1 cut(s) 17
SmlI CTYRAG 1 cut(s) 172
SmoI CTYRAG 1 cut(s) 172
SsiI CCGC 3 cut(s) 299, 330, 332
SspMI CTAG 1 cut(s) 156
SstI GAGCTC 1 cut(s) 170
StyI CCWWGG 1 cut(s) 121
TaaI ACNGT 2 cut(s) 19, 222
TaiI ACGT 1 cut(s) 60
TaqI TCGA 1 cut(s) 324
TauI GCSGC 1 cut(s) 332
TfiI GAWTC 1 cut(s) 306
TscAI CASTG 3 cut(s) 24, 115, 225
TseFI GTSAC 1 cut(s) 58
TseI GCWGC 3 cut(s) 8, 263, 344
Tsp45I GTSAC 1 cut(s) 58
TspGWI ACGGA 2 cut(s) 148, 210
TspRI CASTG 3 cut(s) 24, 115, 225
VneI GTGCAC 1 cut(s) 216
XagI CCTNNNNNAGG 1 cut(s) 154
XspI CTAG 1 cut(s) 156
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.