MD05G1099900.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0011131
Physical Location & Seq
Reverse (-)
20728209 .. 20731419
3211 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1099900.v1.1.491

Sequence Viewer

Length: 990 bp
ATGTCGCGACGAGACAGTCGGGATTCCGACTCCAGACGACACCGTTCCAGGTTTGACAGAGAACCCAGTCCCAAGAGGTCCCGGAGGGGTGAGAAAGACGAAAAAGACAGAATCGCCAGCAAAGGTAATTTAGAAAGTGGGAAGCAGAGTGACCAGGATCAGAAGCAACGCCAGAGGCTGCAAGATACACTGCCACTTGAATCTACATTAGCACCTGACTCAAAGGGGGAAAATGGGGATTCCAGAAAAGAATCCAATAAGAAACCCAGTGGACCTCATGAAGGAACCAAACACTCCACTAATCCATCTGAAATACCCCGGTCTCGATCTTATTTTCAGCACAATGAGCGTGGTAATGCTGGGCAAGTTGTTCGAAGCTCTGGTCGTGGTTCAACTGCTGGTGAGCATGGATCGTGGAGGGATCCAAAGGATAGGCATGATGATAGGACAGTGAGCAAGACAACAACTAATGATTCACGGTCAAGAAATGAGAAAGCTAAGGCGGGTGAAAACAGGAACTGGCGCCATGATGGGTTCTTTGAAATGGAGGCTGATCCACCATCGGCAAGGAAGAGACCTGCATTCAGGGAGAAGAAGATTCCAGTGGAGTCTGATAATGCTGACAAGACAGCAGCAGAGACTGCAAAGTCAAGCCATCCTGACCCTTCCATGGAAGGAAGCAGAAAAAAGGAGGACAGAGGGCACAACCCACGCCACACAGATAGATCTGATAAGCAATTTGCAGGAGAGAGAGGGTCTTACCGAAGAGATGCACCACCGGGTGTCTTTCCTTCGAGAGAGAGGTACACTGGTGGCGCTGGCCGTAACAACAGGGGAAGAGATGGATTCAGTGGGAGACAAGGTTATAATTCTAGTATGGGTCGTGCTGAGAAATGGACGCATGATTTGTATAACGAGGCTAATAGGAGTCCAACGCCGAAAAATGAAGAGGATCAAATTGCAAAGGTGGAAGCACTTTTGGCTTCATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Btz

Protein Analysis

330

Amino Acids

37.22

Weight (kDa)

9.95

Isoelectric Point (pI)

59.2

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011131)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G19650
fragaria_vesca FvH4_2g03741 FvH4_2g04340 FvH4_2g04340
malus_domestica MD05G1099900.v1.1
prunus_persica Prupe.8G144600_v2.0.a1 Prupe.8G144600_v2.0.a1
pyrus_communis pycom05g09870
rosa_chinensis RchiOBHm_Chr6g0253781
rosa_laevigata RLG00000014982 RLG00000014983
rosa_multiflora Rmu_co8218150.1_g000001 Rmu_sc0001351.1_g000015
rosa_roxburghii Rroxscaffold_7G00211640
rosa_rugosa Rorug05G0546400
rosa_samantha Rh6AG062100 Rh6BG055600 Rh6CG055500 Rh6DG053100
rosa_wichuraiana Rw6G005520

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 867
AasI GACNNNNNNGTC 2 cut(s) 15, 646
Acc36I ACCTGC 1 cut(s) 586
AccB1I GGYRCC 1 cut(s) 522
AccII CGCG 1 cut(s) 7
AciI CCGC 1 cut(s) 503
AclWI GGATC 6 cut(s) 165, 416, 418, 429, 548, 960
AcoI YGGCCR 1 cut(s) 820
AcyI GRCGYC 1 cut(s) 523
AdeI CACNNNGTG 1 cut(s) 782
AfaI GTAC 1 cut(s) 806
AfiI CCNNNNNNNGG 2 cut(s) 281, 670
AgsI TTSAA 3 cut(s) 200, 393, 542
AjnI CCWGG 2 cut(s) 47, 153
AluBI AGCT 2 cut(s) 378, 497
AluI AGCT 2 cut(s) 378, 497
Alw26I GTCTC 5 cut(s) 6, 327, 568, 632, 850
AlwI GGATC 6 cut(s) 165, 416, 418, 429, 548, 960
AlwNI CAGNNNCTG 3 cut(s) 178, 519, 641
AoxI GGCC 1 cut(s) 820
ApeKI GCWGC 2 cut(s) 178, 632
ArsI GACNNNNNNTTYG 4 cut(s) 367, 399, 889, 921
AspLEI GCGC 2 cut(s) 525, 818
AspS9I GGNCC 2 cut(s) 78, 272
AsuC2I CCSGG 3 cut(s) 82, 319, 780
AsuHPI GGTGA 3 cut(s) 101, 413, 518
AsuII TTCGAA 1 cut(s) 373
AvaII GGWCC 2 cut(s) 78, 272
BaeGI GKGCMC 1 cut(s) 705
BaeI ACNNNNGTAYC 2 cut(s) 796, 829
BamHI GGATCC 1 cut(s) 421
BanI GGYRCC 1 cut(s) 522
BbvI GCAGC 2 cut(s) 165, 644
BccI CCATC 5 cut(s) 313, 524, 568, 663, 836
BceAI ACGGC 1 cut(s) 807
BcgI CGANNNNNNTGC 2 cut(s) 353, 387
BciT130I CCWGG 2 cut(s) 49, 155
BcnI CCSGG 3 cut(s) 82, 319, 780
BcoDI GTCTC 5 cut(s) 6, 327, 568, 632, 850
BfaI CTAG 1 cut(s) 873
BfoI RGCGCY 2 cut(s) 526, 819
BfuAI ACCTGC 1 cut(s) 586
BglII AGATCT 1 cut(s) 725
BisI GCNGC 2 cut(s) 179, 633
BlsI GCNGC 2 cut(s) 180, 634
Bme1390I CCNGG 5 cut(s) 49, 82, 155, 319, 780
Bme18I GGWCC 2 cut(s) 78, 272
BmgT120I GGNCC 2 cut(s) 78, 272
BmiI GGNNCC 4 cut(s) 80, 286, 423, 524
BmrFI CCNGG 5 cut(s) 49, 82, 155, 319, 780
BmrI ACTGGG 2 cut(s) 60, 261
BmsI GCATC 1 cut(s) 760
BmuI ACTGGG 2 cut(s) 60, 261
BpmI CTGGAG 1 cut(s) 16
Bpu10I CCTNAGC 1 cut(s) 498
Bpu14I TTCGAA 1 cut(s) 373
BpuMI CCSGG 3 cut(s) 82, 319, 780
BsaHI GRCGYC 1 cut(s) 523
BsaI GGTCTC 2 cut(s) 327, 568
BsaJI CCNNGG 2 cut(s) 317, 669
BsaXI ACNNNNNCTCC 2 cut(s) 847, 877
Bsc4I CCNNNNNNNGG 2 cut(s) 281, 670
Bse1I ACTGG 5 cut(s) 66, 267, 524, 602, 814
BseBI CCWGG 2 cut(s) 49, 155
BseDI CCNNGG 2 cut(s) 317, 669
BseGI GGATG 1 cut(s) 655
BseLI CCNNNNNNNGG 2 cut(s) 281, 670
BseMII CTCAG 1 cut(s) 879
BseNI ACTGG 5 cut(s) 66, 267, 524, 602, 814
BseSI GKGCMC 1 cut(s) 705
BseXI GCAGC 2 cut(s) 165, 644
BseYI CCCAGC 1 cut(s) 359
Bsh1236I CGCG 1 cut(s) 7
BshFI GGCC 1 cut(s) 822
BshNI GGYRCC 1 cut(s) 522
BsiSI CCGG 3 cut(s) 82, 319, 779
BslFI GGGAC 2 cut(s) 54, 64
BslI CCNNNNNNNGG 2 cut(s) 281, 670
BsmAI GTCTC 5 cut(s) 6, 327, 568, 632, 850
BsmFI GGGAC 2 cut(s) 54, 64
BsmI GAATGC 1 cut(s) 581
BsnI GGCC 1 cut(s) 822
Bso31I GGTCTC 2 cut(s) 327, 568
Bsp119I TTCGAA 1 cut(s) 373
Bsp1286I GDGCHC 1 cut(s) 705
Bsp143I GATC 7 cut(s) 157, 326, 410, 421, 553, 725, 952
Bsp19I CCATGG 1 cut(s) 669
Bsp68I TCGCGA 1 cut(s) 7
BspACI CCGC 1 cut(s) 503
BspANI GGCC 1 cut(s) 822
BspCNI CTCAG 1 cut(s) 880
BspFNI CGCG 1 cut(s) 7
BspHI TCATGA 1 cut(s) 277
BspLI GGNNCC 4 cut(s) 80, 286, 423, 524
BspMI ACCTGC 1 cut(s) 586
BspPI GGATC 6 cut(s) 165, 416, 418, 429, 548, 960
BspT104I TTCGAA 1 cut(s) 373
BspT107I GGYRCC 1 cut(s) 522
BspTNI GGTCTC 2 cut(s) 327, 568
BsrI ACTGG 5 cut(s) 66, 267, 524, 602, 814
BssECI CCNNGG 2 cut(s) 317, 669
BssMI GATC 7 cut(s) 157, 326, 410, 421, 553, 725, 952
BssNI GRCGYC 1 cut(s) 523
BssT1I CCWWGG 1 cut(s) 669
Bst2UI CCWGG 2 cut(s) 49, 155
Bst4CI ACNGT 4 cut(s) 17, 44, 451, 480
Bst6I CTCTTC 4 cut(s) 566, 760, 832, 942
BstACI GRCGYC 1 cut(s) 523
BstAPI GCANNNNNTGC 1 cut(s) 641
BstBI TTCGAA 1 cut(s) 373
BstC8I GCNNGC 2 cut(s) 118, 820
BstDEI CTNAG 2 cut(s) 498, 888
BstDSI CCRYGG 1 cut(s) 669
BstENI CCTNNNNNAGG 1 cut(s) 279
BstF5I GGATG 1 cut(s) 655
BstFNI CGCG 1 cut(s) 7
BstH2I RGCGCY 2 cut(s) 526, 819
BstHHI GCGC 2 cut(s) 525, 818
BstKTI GATC 7 cut(s) 160, 329, 413, 424, 556, 728, 955
BstMAI GTCTC 5 cut(s) 6, 327, 568, 632, 850
BstMBI GATC 7 cut(s) 157, 326, 410, 421, 553, 725, 952
BstMWI GCNNNNNNNGC 3 cut(s) 346, 641, 980
BstNI CCWGG 2 cut(s) 49, 155
BstSCI CCNGG 5 cut(s) 47, 80, 153, 317, 778
BstSLI GKGCMC 1 cut(s) 705
BstUI CGCG 1 cut(s) 7
BstV1I GCAGC 2 cut(s) 165, 644
BstX2I RGATCY 2 cut(s) 421, 725
BstYI RGATCY 2 cut(s) 421, 725
BsuRI GGCC 1 cut(s) 822
BtgI CCRYGG 1 cut(s) 669
BtsCI GGATG 1 cut(s) 655
BtsI GCAGTG 1 cut(s) 188
BtsIMutI CAGTG 6 cut(s) 188, 274, 456, 609, 807, 856
BtuMI TCGCGA 1 cut(s) 7
BveI ACCTGC 1 cut(s) 586
Cac8I GCNNGC 2 cut(s) 118, 820
CaiI CAGNNNCTG 3 cut(s) 178, 519, 641
CciI TCATGA 1 cut(s) 277
CfoI GCGC 2 cut(s) 525, 818
Cfr13I GGNCC 2 cut(s) 78, 272
CseI GACGC 1 cut(s) 907
Csp6I GTAC 1 cut(s) 805
CspCI CAANNNNNGTGG 2 cut(s) 331, 366
CviAII CATG 6 cut(s) 278, 407, 437, 527, 670, 902
CviJI RGCY 8 cut(s) 178, 378, 497, 551, 654, 822, 920, 983
CviKI_1 RGCY 8 cut(s) 178, 378, 497, 551, 654, 822, 920, 983
CviQI GTAC 1 cut(s) 805
DdeI CTNAG 2 cut(s) 498, 888
DinI GGCGCC 1 cut(s) 524
DpnI GATC 7 cut(s) 159, 328, 412, 423, 555, 727, 954
DpnII GATC 7 cut(s) 157, 326, 410, 421, 553, 725, 952
DraIII CACNNNGTG 1 cut(s) 782
DrdI GACNNNNNNGTC 2 cut(s) 15, 646
DseDI GACNNNNNNGTC 2 cut(s) 15, 646
EaeI YGGCCR 1 cut(s) 820
Eam1104I CTCTTC 4 cut(s) 566, 760, 832, 942
EarI CTCTTC 4 cut(s) 566, 760, 832, 942
Eco130I CCWWGG 1 cut(s) 669
Eco31I GGTCTC 2 cut(s) 327, 568
Eco47I GGWCC 2 cut(s) 78, 272
EcoNI CCTNNNNNAGG 1 cut(s) 279
EcoO109I RGGNCCY 1 cut(s) 78
EcoRII CCWGG 2 cut(s) 47, 153
EcoT14I CCWWGG 1 cut(s) 669
EgeI GGCGCC 1 cut(s) 524
EheI GGCGCC 1 cut(s) 524
ErhI CCWWGG 1 cut(s) 669
FaeI CATG 6 cut(s) 281, 410, 440, 530, 673, 905
FaqI GGGAC 2 cut(s) 54, 64
FatI CATG 6 cut(s) 277, 406, 436, 526, 669, 901
FauI CCCGC 1 cut(s) 496
Fnu4HI GCNGC 2 cut(s) 179, 633
FokI GGATG 1 cut(s) 642
Fsp4HI GCNGC 2 cut(s) 179, 633
FspBI CTAG 1 cut(s) 873
GlaI GCGC 2 cut(s) 524, 817
GluI GCNGC 2 cut(s) 179, 633
GsaI CCCAGC 1 cut(s) 363
GsuI CTGGAG 1 cut(s) 16
HaeII RGCGCY 2 cut(s) 526, 819
HaeIII GGCC 1 cut(s) 822
HapII CCGG 3 cut(s) 82, 319, 779
HgaI GACGC 1 cut(s) 907
HhaI GCGC 2 cut(s) 525, 818
Hin1I GRCGYC 1 cut(s) 523
Hin1II CATG 6 cut(s) 281, 410, 440, 530, 673, 905
Hin6I GCGC 2 cut(s) 523, 816
HinP1I GCGC 2 cut(s) 523, 816
HpaII CCGG 3 cut(s) 82, 319, 779
HphI GGTGA 3 cut(s) 101, 413, 518
Hpy166II GTNNAC 2 cut(s) 272, 807
Hpy188I TCNGA 5 cut(s) 28, 162, 310, 613, 730
Hpy188III TCNNGA 9 cut(s) 6, 20, 33, 243, 278, 324, 483, 659, 795
Hpy8I GTNNAC 2 cut(s) 272, 807
Hpy99I CGWCG 1 cut(s) 12
HpyAV CCTTC 4 cut(s) 275, 668, 675, 801
HpyCH4III ACNGT 4 cut(s) 17, 44, 451, 480
HpyCH4V TGCA 6 cut(s) 181, 581, 644, 743, 773, 962
HpyF10VI GCNNNNNNNGC 3 cut(s) 346, 641, 980
HpyF3I CTNAG 2 cut(s) 498, 888
Hsp92I GRCGYC 1 cut(s) 523
Hsp92II CATG 6 cut(s) 281, 410, 440, 530, 673, 905
HspAI GCGC 2 cut(s) 523, 816
KasI GGCGCC 1 cut(s) 522
Kzo9I GATC 7 cut(s) 157, 326, 410, 421, 553, 725, 952
Lsp1109I GCAGC 2 cut(s) 165, 644
LweI GCATC 1 cut(s) 760
MaeI CTAG 1 cut(s) 873
MaeIII GTNAC 2 cut(s) 149, 824
MalI GATC 7 cut(s) 159, 328, 412, 423, 555, 727, 954
MboI GATC 7 cut(s) 157, 326, 410, 421, 553, 725, 952
MboII GAAGA 6 cut(s) 583, 604, 607, 777, 849, 959
MflI RGATCY 2 cut(s) 421, 725
MhlI GDGCHC 1 cut(s) 705
MluCI AATT 4 cut(s) 127, 737, 868, 957
Mly113I GGCGCC 1 cut(s) 523
MlyI GAGTC 4 cut(s) 23, 212, 617, 937
MmeI TCCRAC 2 cut(s) 51, 956
MspI CCGG 3 cut(s) 82, 319, 779
MspR9I CCNGG 5 cut(s) 49, 82, 155, 319, 780
Mva1269I GAATGC 1 cut(s) 581
MvaI CCWGG 2 cut(s) 49, 155
MvnI CGCG 1 cut(s) 7
MwoI GCNNNNNNNGC 3 cut(s) 346, 641, 980
NarI GGCGCC 1 cut(s) 523
NciI CCSGG 3 cut(s) 82, 319, 780
NcoI CCATGG 1 cut(s) 669
NdeII GATC 7 cut(s) 157, 326, 410, 421, 553, 725, 952
NlaIII CATG 6 cut(s) 281, 410, 440, 530, 673, 905
NlaIV GGNNCC 4 cut(s) 80, 286, 423, 524
NmuCI GTSAC 1 cut(s) 149
NruI TCGCGA 1 cut(s) 7
NspV TTCGAA 1 cut(s) 373
PagI TCATGA 1 cut(s) 277
PctI GAATGC 1 cut(s) 581
PfeI GAWTC 8 cut(s) 23, 111, 200, 239, 251, 473, 598, 846
PfoI TCCNGGA 1 cut(s) 80
PkrI GCNGC 2 cut(s) 180, 634
PleI GAGTC 4 cut(s) 23, 212, 616, 936
PluTI GGCGCC 1 cut(s) 526
PpsI GAGTC 4 cut(s) 23, 212, 616, 936
PpuMI RGGWCCY 1 cut(s) 78
PsiI TTATAA 1 cut(s) 867
Psp5II RGGWCCY 1 cut(s) 78
Psp6I CCWGG 2 cut(s) 47, 153
PspFI CCCAGC 1 cut(s) 359
PspGI CCWGG 2 cut(s) 47, 153
PspN4I GGNNCC 4 cut(s) 80, 286, 423, 524
PspPI GGNCC 2 cut(s) 78, 272
PspPPI RGGWCCY 1 cut(s) 78
PstNI CAGNNNCTG 3 cut(s) 178, 519, 641
PsuI RGATCY 2 cut(s) 421, 725
RruI TCGCGA 1 cut(s) 7
RsaI GTAC 1 cut(s) 806
RsaNI GTAC 1 cut(s) 805
SatI GCNGC 2 cut(s) 179, 633
Sau3AI GATC 7 cut(s) 157, 326, 410, 421, 553, 725, 952
Sau96I GGNCC 2 cut(s) 78, 272
SchI GAGTC 4 cut(s) 23, 212, 617, 937
ScrFI CCNGG 5 cut(s) 49, 82, 155, 319, 780
SduI GDGCHC 1 cut(s) 705
SfaNI GCATC 1 cut(s) 760
SfoI GGCGCC 1 cut(s) 524
SfuI TTCGAA 1 cut(s) 373
SinI GGWCC 2 cut(s) 78, 272
Sse9I AATT 4 cut(s) 127, 737, 868, 957
SsiI CCGC 1 cut(s) 503
SspDI GGCGCC 1 cut(s) 522
SspMI CTAG 1 cut(s) 873
StyD4I CCNGG 5 cut(s) 47, 80, 153, 317, 778
StyI CCWWGG 1 cut(s) 669
TaaI ACNGT 4 cut(s) 17, 44, 451, 480
TaqI TCGA 3 cut(s) 325, 373, 794
TasI AATT 4 cut(s) 127, 737, 868, 957
TfiI GAWTC 8 cut(s) 23, 111, 200, 239, 251, 473, 598, 846
TscAI CASTG 6 cut(s) 195, 274, 456, 609, 814, 856
TseFI GTSAC 1 cut(s) 149
TseI GCWGC 2 cut(s) 178, 632
Tsp45I GTSAC 1 cut(s) 149
TspDTI ATGAA 3 cut(s) 294, 960, 975
TspRI CASTG 6 cut(s) 195, 274, 456, 609, 814, 856
VpaK11BI GGWCC 2 cut(s) 78, 272
XagI CCTNNNNNAGG 1 cut(s) 279
XspI CTAG 1 cut(s) 873
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.