MD05G1130500.v1.1
NAC Family

NAC domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0010486
Physical Location & Seq
Forward (+)
25567413 .. 25569020
1608 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1130500.v1.1.491

Sequence Viewer

Length: 879 bp
ATGGCAGCCAACACCATAAATCCTGATCATGAAATGACTCCGGAATTCGAACTTCCGGGATTTCGGTTCCATCCAACAGAAGAAGAACTTCTTGAATTCTACCTTAGAAGCATGGTTTTTGGCAAGAGGTTGCGTTTTGACATAATTGGTTTCCTCAATATCTACCATCATGACCCTTGGGACTTACCTGGGTTGTCAAAGATCGGAGAAAGGGAGTGGTATTTTTTTGTGCCAAGGGACAGGAAGCATGGCAGTGGAGGAAGGCCTAACAGAACCACTGAAACTGGGTTTTGGAAAGCCACCGGCTCTGACCGCAAGATCGTGAGCCTCTCGGATCCAAAGAGGATCATAGGGTTGAGAAAGACACTTGTTTTTTACAAAGGGAGAGCCCCAAGAGGAAGCAAGACTGATTGGGTCATGAACGAGTATCGTTTGCCTGATAACTGCCAGTATCTCAAGGATATTGTACTCTGCAAGATATATAGAAAGGCAACTTCCTTGAAAGTGCTAGAGCAAAGGGCAGCAATGGAGGAAAATCAGATAAAGGACTTTCATGCTTCCCCTAACTCATCATCTCCAACATCCATGGAGACATTCTCACTTGGCAGCCAAGCAATACAGGAAGCTCTGACTCCACAAATTCCCACAAAGCATGTAGTATTCAAGCAAGAAGTGGAAGATGCAACTCTAGTGGTAGCGCAAGAGCAAAAGGGTGAGAATGCAATGGAGATTAAAGGGTCTCCTCATCCCATATACAATTTACCAGAACTCCAAGTGCCTAAATTTAGCATGGACTGGACCCAAGACACAGTTTGGACCCAAATGAGTAGCCCTTGGCTTCAAAACTTGACCCCTTTGGCGAACATTCTCAACTTCTAA

Protein Analysis

293

Amino Acids

33.71

Weight (kDa)

7.1

Isoelectric Point (pI)

39.61

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NAM PF02365 19 - 145 1.8e-44 No apical meristem (NAM) protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010486)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G17040
fragaria_vesca FvH4_2g16180 FvH4_2g16180
malus_domestica MD05G1130500.v1.1 MD10G1133400.v1.1
prunus_persica Prupe.8G174800_v2.0.a1
pyrus_communis pycom05g12740 pycom10g11550
rosa_chinensis RchiOBHm_Chr1g0324811 RchiOBHm_Chr6g0279801
rosa_laevigata RLG00000013115
rosa_multiflora Rmu_sc0002340.1_g000019
rosa_roxburghii Rroxscaffold_7G00188620
rosa_rugosa Rorug06G0128400
rosa_samantha Rh1CG065600 Rh6AG236500 Rh6BG240700 Rh6CG243100 Rh6DG234300
rosa_wichuraiana Rw1G005380 Rw6G020610

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccIII TCCGGA 1 cut(s) 40
AciI CCGC 1 cut(s) 313
AclWI GGATC 3 cut(s) 329, 342, 353
AcsI RAATTY 4 cut(s) 44, 95, 639, 782
AfaI GTAC 1 cut(s) 468
AgsI TTSAA 4 cut(s) 95, 502, 664, 842
AjnI CCWGG 1 cut(s) 187
AluBI AGCT 1 cut(s) 626
AluI AGCT 1 cut(s) 626
Alw26I GTCTC 2 cut(s) 584, 744
AlwI GGATC 3 cut(s) 329, 342, 353
Aor13HI TCCGGA 1 cut(s) 40
AoxI GGCC 1 cut(s) 263
ApeKI GCWGC 3 cut(s) 5, 521, 606
ApoI RAATTY 4 cut(s) 44, 95, 639, 782
Asp700I GAANNNNTTC 1 cut(s) 87
AspLEI GCGC 1 cut(s) 700
AspS9I GGNCC 2 cut(s) 798, 816
AsuC2I CCSGG 1 cut(s) 57
AsuHPI GGTGA 1 cut(s) 725
AsuII TTCGAA 1 cut(s) 48
AvaII GGWCC 2 cut(s) 798, 816
BamHI GGATCC 1 cut(s) 334
BanII GRGCYC 1 cut(s) 391
BbvI GCAGC 3 cut(s) 17, 533, 618
BccI CCATC 2 cut(s) 78, 174
BciT130I CCWGG 1 cut(s) 189
BclI TGATCA 1 cut(s) 25
BcnI CCSGG 1 cut(s) 57
BcoDI GTCTC 2 cut(s) 584, 744
BfaI CTAG 2 cut(s) 509, 689
BisI GCNGC 3 cut(s) 6, 522, 607
BlsI GCNGC 3 cut(s) 7, 523, 608
Bme1390I CCNGG 2 cut(s) 57, 189
Bme18I GGWCC 2 cut(s) 798, 816
BmgT120I GGNCC 2 cut(s) 798, 816
BmiI GGNNCC 4 cut(s) 68, 336, 800, 818
BmrFI CCNGG 2 cut(s) 57, 189
BmrI ACTGGG 1 cut(s) 294
BmsI GCATC 1 cut(s) 670
BmuI ACTGGG 1 cut(s) 294
BplI GAGNNNNNCTC 2 cut(s) 581, 613
Bpu14I TTCGAA 1 cut(s) 48
BpuEI CTTGAG 1 cut(s) 440
BpuMI CCSGG 1 cut(s) 57
BsaI GGTCTC 1 cut(s) 744
BsaJI CCNNGG 5 cut(s) 176, 188, 233, 585, 833
BsaWI WCCGGW 1 cut(s) 40
BsaXI ACNNNNNCTCC 2 cut(s) 753, 783
Bse118I RCCGGY 1 cut(s) 302
Bse1I ACTGG 3 cut(s) 289, 448, 800
Bse3DI GCAATG 2 cut(s) 531, 729
BseAI TCCGGA 1 cut(s) 40
BseBI CCWGG 1 cut(s) 189
BseDI CCNNGG 5 cut(s) 176, 188, 233, 585, 833
BseGI GGATG 3 cut(s) 70, 581, 745
BseMI GCAATG 2 cut(s) 531, 729
BseNI ACTGG 3 cut(s) 289, 448, 800
BseRI GAGGAG 1 cut(s) 732
BseXI GCAGC 3 cut(s) 17, 533, 618
BshFI GGCC 1 cut(s) 265
BsiSI CCGG 3 cut(s) 41, 56, 303
BslFI GGGAC 2 cut(s) 194, 251
BsmAI GTCTC 2 cut(s) 584, 744
BsmFI GGGAC 2 cut(s) 194, 251
BsmI GAATGC 1 cut(s) 724
BsnI GGCC 1 cut(s) 265
Bso31I GGTCTC 1 cut(s) 744
Bsp119I TTCGAA 1 cut(s) 48
Bsp1286I GDGCHC 1 cut(s) 391
Bsp13I TCCGGA 1 cut(s) 40
Bsp143I GATC 5 cut(s) 25, 201, 318, 334, 345
Bsp19I CCATGG 1 cut(s) 585
BspACI CCGC 1 cut(s) 313
BspANI GGCC 1 cut(s) 265
BspEI TCCGGA 1 cut(s) 40
BspHI TCATGA 3 cut(s) 28, 169, 417
BspLI GGNNCC 4 cut(s) 68, 336, 800, 818
BspPI GGATC 3 cut(s) 329, 342, 353
BspT104I TTCGAA 1 cut(s) 48
BspTNI GGTCTC 1 cut(s) 744
BsrDI GCAATG 2 cut(s) 531, 729
BsrFI RCCGGY 1 cut(s) 302
BsrI ACTGG 3 cut(s) 289, 448, 800
BssAI RCCGGY 1 cut(s) 302
BssECI CCNNGG 5 cut(s) 176, 188, 233, 585, 833
BssMI GATC 5 cut(s) 25, 201, 318, 334, 345
BssT1I CCWWGG 4 cut(s) 176, 233, 585, 833
Bst2UI CCWGG 1 cut(s) 189
Bst4CI ACNGT 1 cut(s) 811
BstBI TTCGAA 1 cut(s) 48
BstDEI CTNAG 1 cut(s) 104
BstDSI CCRYGG 1 cut(s) 585
BstF5I GGATG 3 cut(s) 70, 581, 745
BstHHI GCGC 1 cut(s) 700
BstKTI GATC 5 cut(s) 28, 204, 321, 337, 348
BstMAI GTCTC 2 cut(s) 584, 744
BstMBI GATC 5 cut(s) 25, 201, 318, 334, 345
BstMWI GCNNNNNNNGC 1 cut(s) 312
BstNI CCWGG 1 cut(s) 189
BstNSI RCATGY 1 cut(s) 656
BstSCI CCNGG 2 cut(s) 55, 187
BstV1I GCAGC 3 cut(s) 17, 533, 618
BstX2I RGATCY 1 cut(s) 334
BstYI RGATCY 1 cut(s) 334
BsuRI GGCC 1 cut(s) 265
BtgI CCRYGG 1 cut(s) 585
BtsCI GGATG 3 cut(s) 70, 581, 745
BtsI GCAGTG 1 cut(s) 259
BtsIMutI CAGTG 2 cut(s) 259, 276
CciI TCATGA 3 cut(s) 28, 169, 417
CfoI GCGC 1 cut(s) 700
Cfr10I RCCGGY 1 cut(s) 302
Cfr13I GGNCC 2 cut(s) 798, 816
Csp6I GTAC 1 cut(s) 467
CspCI CAANNNNNGTGG 2 cut(s) 672, 707
CviAII CATG 9 cut(s) 29, 112, 170, 248, 418, 554, 586, 653, 790
CviQI GTAC 1 cut(s) 467
DdeI CTNAG 1 cut(s) 104
DpnI GATC 5 cut(s) 27, 203, 320, 336, 347
DpnII GATC 5 cut(s) 25, 201, 318, 334, 345
Eco130I CCWWGG 4 cut(s) 176, 233, 585, 833
Eco147I AGGCCT 1 cut(s) 265
Eco24I GRGCYC 1 cut(s) 391
Eco31I GGTCTC 1 cut(s) 744
Eco47I GGWCC 2 cut(s) 798, 816
EcoRI GAATTC 2 cut(s) 44, 95
EcoRII CCWGG 1 cut(s) 187
EcoT14I CCWWGG 4 cut(s) 176, 233, 585, 833
EcoT38I GRGCYC 1 cut(s) 391
ErhI CCWWGG 4 cut(s) 176, 233, 585, 833
FaeI CATG 9 cut(s) 32, 115, 173, 251, 421, 557, 589, 656, 793
FalI AAGNNNNNCTT 4 cut(s) 72, 104, 75, 107
FaqI GGGAC 2 cut(s) 194, 251
FatI CATG 9 cut(s) 28, 111, 169, 247, 417, 553, 585, 652, 789
FbaI TGATCA 1 cut(s) 25
Fnu4HI GCNGC 3 cut(s) 6, 522, 607
FokI GGATG 3 cut(s) 57, 568, 732
FriOI GRGCYC 1 cut(s) 391
Fsp4HI GCNGC 3 cut(s) 6, 522, 607
FspBI CTAG 2 cut(s) 509, 689
GlaI GCGC 1 cut(s) 699
GluI GCNGC 3 cut(s) 6, 522, 607
HaeIII GGCC 1 cut(s) 265
HapII CCGG 3 cut(s) 41, 56, 303
HhaI GCGC 1 cut(s) 700
Hin1II CATG 9 cut(s) 32, 115, 173, 251, 421, 557, 589, 656, 793
Hin6I GCGC 1 cut(s) 698
HinP1I GCGC 1 cut(s) 698
HinfI GANTC 2 cut(s) 37, 631
HpaII CCGG 3 cut(s) 41, 56, 303
HphI GGTGA 1 cut(s) 725
Hpy188I TCNGA 5 cut(s) 206, 310, 334, 540, 630
Hpy188III TCNNGA 7 cut(s) 23, 29, 41, 92, 170, 322, 418
HpyAV CCTTC 1 cut(s) 255
HpyCH4III ACNGT 1 cut(s) 811
HpyCH4V TGCA 3 cut(s) 474, 683, 722
HpyF10VI GCNNNNNNNGC 1 cut(s) 312
HpyF3I CTNAG 1 cut(s) 104
Hsp92II CATG 9 cut(s) 32, 115, 173, 251, 421, 557, 589, 656, 793
HspAI GCGC 1 cut(s) 698
Kpn2I TCCGGA 1 cut(s) 40
Ksp22I TGATCA 1 cut(s) 25
Kzo9I GATC 5 cut(s) 25, 201, 318, 334, 345
Lsp1109I GCAGC 3 cut(s) 17, 533, 618
LweI GCATC 1 cut(s) 670
MaeI CTAG 2 cut(s) 509, 689
MalI GATC 5 cut(s) 27, 203, 320, 336, 347
MboI GATC 5 cut(s) 25, 201, 318, 334, 345
MboII GAAGA 3 cut(s) 92, 95, 689
MflI RGATCY 1 cut(s) 334
MhlI GDGCHC 1 cut(s) 391
MluCI AATT 6 cut(s) 44, 95, 144, 639, 757, 782
MlyI GAGTC 2 cut(s) 31, 625
MmeI TCCRAC 2 cut(s) 98, 602
MnlI CCTC 8 cut(s) 120, 164, 251, 336, 338, 389, 523, 753
MroI TCCGGA 1 cut(s) 40
MroXI GAANNNNTTC 1 cut(s) 87
MseI TTAA 1 cut(s) 732
MslI CAYNNNNRTG 1 cut(s) 252
MspI CCGG 3 cut(s) 41, 56, 303
MspR9I CCNGG 2 cut(s) 57, 189
Mva1269I GAATGC 1 cut(s) 724
MvaI CCWGG 1 cut(s) 189
MwoI GCNNNNNNNGC 1 cut(s) 312
NciI CCSGG 1 cut(s) 57
NcoI CCATGG 1 cut(s) 585
NdeII GATC 5 cut(s) 25, 201, 318, 334, 345
NlaIII CATG 9 cut(s) 32, 115, 173, 251, 421, 557, 589, 656, 793
NlaIV GGNNCC 4 cut(s) 68, 336, 800, 818
NspI RCATGY 1 cut(s) 656
NspV TTCGAA 1 cut(s) 48
PagI TCATGA 3 cut(s) 28, 169, 417
PceI AGGCCT 1 cut(s) 265
PctI GAATGC 1 cut(s) 724
PdmI GAANNNNTTC 1 cut(s) 87
PfoI TCCNGGA 1 cut(s) 55
PkrI GCNGC 3 cut(s) 7, 523, 608
PleI GAGTC 2 cut(s) 31, 625
PpsI GAGTC 2 cut(s) 31, 625
Psp6I CCWGG 1 cut(s) 187
PspGI CCWGG 1 cut(s) 187
PspN4I GGNNCC 4 cut(s) 68, 336, 800, 818
PspPI GGNCC 2 cut(s) 798, 816
PsuI RGATCY 1 cut(s) 334
RsaI GTAC 1 cut(s) 468
RsaNI GTAC 1 cut(s) 467
RseI CAYNNNNRTG 1 cut(s) 252
SaqAI TTAA 1 cut(s) 732
SatI GCNGC 3 cut(s) 6, 522, 607
Sau3AI GATC 5 cut(s) 25, 201, 318, 334, 345
Sau96I GGNCC 2 cut(s) 798, 816
SchI GAGTC 2 cut(s) 31, 625
ScrFI CCNGG 2 cut(s) 57, 189
SduI GDGCHC 1 cut(s) 391
SetI ASST 4 cut(s) 105, 131, 190, 628
SfaNI GCATC 1 cut(s) 670
SfuI TTCGAA 1 cut(s) 48
SinI GGWCC 2 cut(s) 798, 816
SmiMI CAYNNNNRTG 1 cut(s) 252
SmlI CTYRAG 1 cut(s) 455
SmoI CTYRAG 1 cut(s) 455
Sse9I AATT 6 cut(s) 44, 95, 144, 639, 757, 782
SseBI AGGCCT 1 cut(s) 265
SsiI CCGC 1 cut(s) 313
SspMI CTAG 2 cut(s) 509, 689
StuI AGGCCT 1 cut(s) 265
StyD4I CCNGG 2 cut(s) 55, 187
StyI CCWWGG 4 cut(s) 176, 233, 585, 833
TaaI ACNGT 1 cut(s) 811
TaqI TCGA 1 cut(s) 48
TasI AATT 6 cut(s) 44, 95, 144, 639, 757, 782
TatI WGTACW 1 cut(s) 466
Tru1I TTAA 1 cut(s) 732
Tru9I TTAA 1 cut(s) 732
TscAI CASTG 2 cut(s) 259, 283
TseI GCWGC 3 cut(s) 5, 521, 606
TspDTI ATGAA 3 cut(s) 45, 434, 542
TspRI CASTG 2 cut(s) 259, 283
VpaK11BI GGWCC 2 cut(s) 798, 816
XapI RAATTY 4 cut(s) 44, 95, 639, 782
XceI RCATGY 1 cut(s) 656
XmnI GAANNNNTTC 1 cut(s) 87
XspI CTAG 2 cut(s) 509, 689
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.