MD10G1133400.v1.1
NAC Family

NAC domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0010486
Physical Location & Seq
Forward (+)
21527167 .. 21528831
1665 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1133400.v1.1.491

Sequence Viewer

Length: 885 bp
ATGGCAGCCAACACTATAGAACCTGATCACCAAATGACCCCAGAATTCGAACTTCCAGGGTTTCGATTCCATCCCACAGAAGAAGAACTCCTTGAATTCTACCTGAAGAGTATGGTTTTTGGCAAGAGATTGCGTTTCGACATAATTGGTTTTCTCAATATCTACCATCATGACCCTTGGGACTTACCTGGGTTGTCAAAGATTGGAGAAAGGGAGTGGTATTTTTTTGTGCCCAGGGACAGGAAGCATGGCAGTGGAGGAAGGCCTAACAGAACCACTGAAACTGGGTTTTGGAAAGCCACAGGCTCTGACCGGAAGATTGTGAGCCTCTCGAATCCAAAGAGGATCATAGGGTTGAGAAAGACTCTTGTTTTTTACAAAGGGAGAGCTCCAAGAGGAAGTAAGACTGATTGGGTCATGAACGAGTATCGTTTGCCTGATAACTGCCAGTATCTCAAAGATATTGTGCTGTGCAAGATATATAGAAAGGCAACTTCCTTGAAAGTGTTAGAGCAAATGGCAGCAATAGAGGGAGATCAGATAAAGAACTTGCATGCTTCCCCTAACTCATCACCTCCTCCAACATCCATGGATACCATCTCATTTTGCAGCCAAGCATTACAGGAAGATCTGACTCCGCAAATTTCCACAAACCATGTGGTATTCAAGCAAGAAATAGAAGATGCAGCTCTGGTAATAGCACAGGAGCAAAAGGATGAGAATGCAATGGAGATCAAAGGGTCTCCTCCTCCTCCCATGTACAATCTACCAGAGCTCCAAGTGCCTAAATTTAGCATGGACTGGACCCAAGACACAGTTTGGACCCAAATGAATAGCCCTTGGCTTCAAAACTTGACCCCTTTGGCCAATATTCTCAACTTCTAA

Protein Analysis

295

Amino Acids

33.86

Weight (kDa)

6.4

Isoelectric Point (pI)

44.67

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NAM PF02365 19 - 145 1.7e-44 No apical meristem (NAM) protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010486)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G17040
fragaria_vesca FvH4_2g16180 FvH4_2g16180
malus_domestica MD05G1130500.v1.1 MD10G1133400.v1.1
prunus_persica Prupe.8G174800_v2.0.a1
pyrus_communis pycom05g12740 pycom10g11550
rosa_chinensis RchiOBHm_Chr1g0324811 RchiOBHm_Chr6g0279801
rosa_laevigata RLG00000013115
rosa_multiflora Rmu_sc0002340.1_g000019
rosa_roxburghii Rroxscaffold_7G00188620
rosa_rugosa Rorug06G0128400
rosa_samantha Rh1CG065600 Rh6AG236500 Rh6BG240700 Rh6CG243100 Rh6DG234300
rosa_wichuraiana Rw1G005380 Rw6G020610

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 638
AclWI GGATC 1 cut(s) 353
AcoI YGGCCR 1 cut(s) 864
AcsI RAATTY 4 cut(s) 44, 95, 642, 788
AcuI CTGAAG 1 cut(s) 125
AfaI GTAC 1 cut(s) 761
AfiI CCNNNNNNNGG 1 cut(s) 240
AgsI TTSAA 4 cut(s) 95, 502, 667, 848
AjnI CCWGG 3 cut(s) 55, 187, 233
AluBI AGCT 3 cut(s) 389, 689, 775
AluI AGCT 3 cut(s) 389, 689, 775
Alw21I GWGCWC 2 cut(s) 391, 777
Alw26I GTCTC 1 cut(s) 747
AlwI GGATC 1 cut(s) 353
AlwNI CAGNNNCTG 1 cut(s) 308
AoxI GGCC 2 cut(s) 263, 864
ApeKI GCWGC 4 cut(s) 5, 521, 609, 686
ApoI RAATTY 4 cut(s) 44, 95, 642, 788
AspS9I GGNCC 2 cut(s) 804, 822
AsuHPI GGTGA 2 cut(s) 20, 564
AsuII TTCGAA 1 cut(s) 48
AvaII GGWCC 2 cut(s) 804, 822
BaeGI GKGCMC 1 cut(s) 234
BalI TGGCCA 1 cut(s) 866
BanII GRGCYC 2 cut(s) 391, 777
Bbv12I GWGCWC 2 cut(s) 391, 777
BbvI GCAGC 4 cut(s) 17, 533, 621, 698
BccI CCATC 3 cut(s) 78, 174, 605
BciT130I CCWGG 3 cut(s) 57, 189, 235
BciVI GTATCC 1 cut(s) 586
BclI TGATCA 1 cut(s) 25
BcoDI GTCTC 1 cut(s) 747
BfmI CTRYAG 1 cut(s) 15
BfuI GTATCC 1 cut(s) 586
BglII AGATCT 1 cut(s) 628
BisI GCNGC 4 cut(s) 6, 522, 610, 687
BlsI GCNGC 4 cut(s) 7, 523, 611, 688
Bme1390I CCNGG 3 cut(s) 57, 189, 235
Bme18I GGWCC 2 cut(s) 804, 822
BmgT120I GGNCC 2 cut(s) 804, 822
BmiI GGNNCC 2 cut(s) 806, 824
BmrFI CCNGG 3 cut(s) 57, 189, 235
BmrI ACTGGG 1 cut(s) 294
BmsI GCATC 1 cut(s) 673
BmuI ACTGGG 1 cut(s) 294
BplI GAGNNNNNCTC 2 cut(s) 349, 381
Bpu14I TTCGAA 1 cut(s) 48
BsaI GGTCTC 1 cut(s) 747
BsaJI CCNNGG 7 cut(s) 56, 176, 188, 233, 234, 588, 839
BsaWI WCCGGW 1 cut(s) 312
BsaXI ACNNNNNCTCC 2 cut(s) 759, 789
Bsc4I CCNNNNNNNGG 1 cut(s) 240
Bse1I ACTGG 3 cut(s) 289, 448, 806
Bse3DI GCAATG 1 cut(s) 732
BseBI CCWGG 3 cut(s) 57, 189, 235
BseDI CCNNGG 7 cut(s) 56, 176, 188, 233, 234, 588, 839
BseGI GGATG 3 cut(s) 70, 584, 721
BseLI CCNNNNNNNGG 1 cut(s) 240
BseMI GCAATG 1 cut(s) 732
BseNI ACTGG 3 cut(s) 289, 448, 806
BseRI GAGGAG 4 cut(s) 567, 735, 738, 741
BseSI GKGCMC 1 cut(s) 234
BseXI GCAGC 4 cut(s) 17, 533, 621, 698
BshFI GGCC 2 cut(s) 265, 866
BsiHKAI GWGCWC 2 cut(s) 391, 777
BsiSI CCGG 1 cut(s) 313
BslFI GGGAC 2 cut(s) 194, 251
BslI CCNNNNNNNGG 1 cut(s) 240
BsmAI GTCTC 1 cut(s) 747
BsmFI GGGAC 2 cut(s) 194, 251
BsmI GAATGC 1 cut(s) 727
BsnI GGCC 2 cut(s) 265, 866
Bso31I GGTCTC 1 cut(s) 747
Bsp119I TTCGAA 1 cut(s) 48
Bsp1286I GDGCHC 3 cut(s) 234, 391, 777
Bsp1407I TGTACA 1 cut(s) 759
Bsp143I GATC 5 cut(s) 25, 345, 535, 628, 732
Bsp19I CCATGG 1 cut(s) 588
BspACI CCGC 1 cut(s) 638
BspANI GGCC 2 cut(s) 265, 866
BspHI TCATGA 2 cut(s) 169, 417
BspLI GGNNCC 2 cut(s) 806, 824
BspPI GGATC 1 cut(s) 353
BspT104I TTCGAA 1 cut(s) 48
BspTNI GGTCTC 1 cut(s) 747
BsrDI GCAATG 1 cut(s) 732
BsrGI TGTACA 1 cut(s) 759
BsrI ACTGG 3 cut(s) 289, 448, 806
BssECI CCNNGG 7 cut(s) 56, 176, 188, 233, 234, 588, 839
BssMI GATC 5 cut(s) 25, 345, 535, 628, 732
BssT1I CCWWGG 3 cut(s) 176, 588, 839
Bst2UI CCWGG 3 cut(s) 57, 189, 235
Bst4CI ACNGT 1 cut(s) 817
Bst6I CTCTTC 1 cut(s) 101
BstAUI TGTACA 1 cut(s) 759
BstBI TTCGAA 1 cut(s) 48
BstC8I GCNNGC 1 cut(s) 555
BstDSI CCRYGG 1 cut(s) 588
BstF5I GGATG 3 cut(s) 70, 584, 721
BstKTI GATC 5 cut(s) 28, 348, 538, 631, 735
BstMAI GTCTC 1 cut(s) 747
BstMBI GATC 5 cut(s) 25, 345, 535, 628, 732
BstMWI GCNNNNNNNGC 1 cut(s) 781
BstNI CCWGG 3 cut(s) 57, 189, 235
BstNSI RCATGY 1 cut(s) 557
BstSCI CCNGG 3 cut(s) 55, 187, 233
BstSFI CTRYAG 1 cut(s) 15
BstSLI GKGCMC 1 cut(s) 234
BstV1I GCAGC 4 cut(s) 17, 533, 621, 698
BstX2I RGATCY 1 cut(s) 628
BstYI RGATCY 1 cut(s) 628
BsuI GTATCC 1 cut(s) 586
BsuRI GGCC 2 cut(s) 265, 866
BtgI CCRYGG 1 cut(s) 588
BtsCI GGATG 3 cut(s) 70, 584, 721
BtsI GCAGTG 1 cut(s) 259
BtsIMutI CAGTG 2 cut(s) 259, 276
Cac8I GCNNGC 1 cut(s) 555
CaiI CAGNNNCTG 1 cut(s) 308
CciI TCATGA 2 cut(s) 169, 417
Cfr13I GGNCC 2 cut(s) 804, 822
Csp6I GTAC 1 cut(s) 760
CspCI CAANNNNNGTGG 2 cut(s) 639, 674
CviAII CATG 8 cut(s) 170, 248, 418, 554, 589, 656, 757, 796
CviQI GTAC 1 cut(s) 760
DpnI GATC 5 cut(s) 27, 347, 537, 630, 734
DpnII GATC 5 cut(s) 25, 345, 535, 628, 732
EaeI YGGCCR 1 cut(s) 864
Eam1104I CTCTTC 1 cut(s) 101
EarI CTCTTC 1 cut(s) 101
Ecl136II GAGCTC 2 cut(s) 389, 775
Eco130I CCWWGG 3 cut(s) 176, 588, 839
Eco147I AGGCCT 1 cut(s) 265
Eco24I GRGCYC 2 cut(s) 391, 777
Eco31I GGTCTC 1 cut(s) 747
Eco47I GGWCC 2 cut(s) 804, 822
Eco53kI GAGCTC 2 cut(s) 389, 775
Eco57I CTGAAG 1 cut(s) 125
EcoICRI GAGCTC 2 cut(s) 389, 775
EcoRI GAATTC 2 cut(s) 44, 95
EcoRII CCWGG 3 cut(s) 55, 187, 233
EcoT14I CCWWGG 3 cut(s) 176, 588, 839
EcoT38I GRGCYC 2 cut(s) 391, 777
ErhI CCWWGG 3 cut(s) 176, 588, 839
FaeI CATG 8 cut(s) 173, 251, 421, 557, 592, 659, 760, 799
FalI AAGNNNNNCTT 2 cut(s) 75, 107
FaqI GGGAC 2 cut(s) 194, 251
FatI CATG 8 cut(s) 169, 247, 417, 553, 588, 655, 756, 795
FbaI TGATCA 1 cut(s) 25
Fnu4HI GCNGC 4 cut(s) 6, 522, 610, 687
FokI GGATG 3 cut(s) 57, 571, 728
FriOI GRGCYC 2 cut(s) 391, 777
Fsp4HI GCNGC 4 cut(s) 6, 522, 610, 687
GluI GCNGC 4 cut(s) 6, 522, 610, 687
HaeIII GGCC 2 cut(s) 265, 866
HapII CCGG 1 cut(s) 313
Hin1II CATG 8 cut(s) 173, 251, 421, 557, 592, 659, 760, 799
HinfI GANTC 4 cut(s) 66, 334, 364, 634
HpaII CCGG 1 cut(s) 313
HphI GGTGA 2 cut(s) 20, 564
Hpy188I TCNGA 3 cut(s) 310, 540, 633
Hpy188III TCNNGA 3 cut(s) 170, 331, 418
HpyAV CCTTC 1 cut(s) 255
HpyCH4III ACNGT 1 cut(s) 817
HpyCH4V TGCA 5 cut(s) 474, 553, 609, 686, 725
HpyF10VI GCNNNNNNNGC 1 cut(s) 781
Hsp92II CATG 8 cut(s) 173, 251, 421, 557, 592, 659, 760, 799
Ksp22I TGATCA 1 cut(s) 25
Kzo9I GATC 5 cut(s) 25, 345, 535, 628, 732
LmnI GCTCC 3 cut(s) 394, 706, 780
Lsp1109I GCAGC 4 cut(s) 17, 533, 621, 698
LweI GCATC 1 cut(s) 673
MalI GATC 5 cut(s) 27, 347, 537, 630, 734
MboI GATC 5 cut(s) 25, 345, 535, 628, 732
MboII GAAGA 6 cut(s) 92, 95, 118, 328, 638, 692
MflI RGATCY 1 cut(s) 628
MhlI GDGCHC 3 cut(s) 234, 391, 777
MlsI TGGCCA 1 cut(s) 866
MluCI AATT 5 cut(s) 44, 95, 144, 642, 788
MluNI TGGCCA 1 cut(s) 866
MlyI GAGTC 2 cut(s) 358, 628
MmeI TCCRAC 1 cut(s) 605
Mox20I TGGCCA 1 cut(s) 866
MscI TGGCCA 1 cut(s) 866
MslI CAYNNNNRTG 1 cut(s) 252
Msp20I TGGCCA 1 cut(s) 866
MspI CCGG 1 cut(s) 313
MspR9I CCNGG 3 cut(s) 57, 189, 235
Mva1269I GAATGC 1 cut(s) 727
MvaI CCWGG 3 cut(s) 57, 189, 235
MwoI GCNNNNNNNGC 1 cut(s) 781
NcoI CCATGG 1 cut(s) 588
NdeII GATC 5 cut(s) 25, 345, 535, 628, 732
NlaIII CATG 8 cut(s) 173, 251, 421, 557, 592, 659, 760, 799
NlaIV GGNNCC 2 cut(s) 806, 824
NspI RCATGY 1 cut(s) 557
NspV TTCGAA 1 cut(s) 48
PaeI GCATGC 1 cut(s) 557
PagI TCATGA 2 cut(s) 169, 417
PasI CCCWGGG 1 cut(s) 234
PceI AGGCCT 1 cut(s) 265
PctI GAATGC 1 cut(s) 727
PfeI GAWTC 2 cut(s) 66, 334
PkrI GCNGC 4 cut(s) 7, 523, 611, 688
PleI GAGTC 2 cut(s) 358, 628
PpsI GAGTC 2 cut(s) 358, 628
Psp124BI GAGCTC 2 cut(s) 391, 777
Psp6I CCWGG 3 cut(s) 55, 187, 233
PspGI CCWGG 3 cut(s) 55, 187, 233
PspN4I GGNNCC 2 cut(s) 806, 824
PspPI GGNCC 2 cut(s) 804, 822
PstNI CAGNNNCTG 1 cut(s) 308
PsuI RGATCY 1 cut(s) 628
RsaI GTAC 1 cut(s) 761
RsaNI GTAC 1 cut(s) 760
RseI CAYNNNNRTG 1 cut(s) 252
SacI GAGCTC 2 cut(s) 391, 777
SatI GCNGC 4 cut(s) 6, 522, 610, 687
Sau3AI GATC 5 cut(s) 25, 345, 535, 628, 732
Sau96I GGNCC 2 cut(s) 804, 822
SchI GAGTC 2 cut(s) 358, 628
ScrFI CCNGG 3 cut(s) 57, 189, 235
SduI GDGCHC 3 cut(s) 234, 391, 777
SetI ASST 7 cut(s) 25, 105, 190, 391, 577, 691, 777
SfaNI GCATC 1 cut(s) 673
SfcI CTRYAG 1 cut(s) 15
SfuI TTCGAA 1 cut(s) 48
SinI GGWCC 2 cut(s) 804, 822
SmiMI CAYNNNNRTG 1 cut(s) 252
SphI GCATGC 1 cut(s) 557
Sse9I AATT 5 cut(s) 44, 95, 144, 642, 788
SseBI AGGCCT 1 cut(s) 265
SsiI CCGC 1 cut(s) 638
SspI AATATT 1 cut(s) 871
SstI GAGCTC 2 cut(s) 391, 777
StuI AGGCCT 1 cut(s) 265
StyD4I CCNGG 3 cut(s) 55, 187, 233
StyI CCWWGG 3 cut(s) 176, 588, 839
TaaI ACNGT 1 cut(s) 817
TaqI TCGA 4 cut(s) 48, 64, 138, 332
TasI AATT 5 cut(s) 44, 95, 144, 642, 788
TatI WGTACW 1 cut(s) 759
TfiI GAWTC 2 cut(s) 66, 334
TscAI CASTG 2 cut(s) 259, 283
TseI GCWGC 4 cut(s) 5, 521, 609, 686
TspDTI ATGAA 2 cut(s) 434, 845
TspRI CASTG 2 cut(s) 259, 283
VpaK11BI GGWCC 2 cut(s) 804, 822
XapI RAATTY 4 cut(s) 44, 95, 642, 788
XceI RCATGY 1 cut(s) 557
XcmI CCANNNNNNNNNTGG 1 cut(s) 655
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.