MD05G1145700.v1.1

Phototropin-2-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0004987
Physical Location & Seq
Reverse (-)
27671862 .. 27682736
10875 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1145700.v1.1.491

Sequence Viewer

Length: 2937 bp
ATGGAGGTCTTGAATCTGAAAGATGTTGGAGCAAACCCTAGCAACAAGCAAGCTGCTGATATTGTGGAAAGTGGATCAGGAAGCACTTCTGCTCCAGCTGGTAGAAGGGATTCCATCAACAAGTGGATGGCATTCAGAACTGATCAGGCTTCGGCTGATCCTAAAAGCACTTCTGCCACTGGTTCAAATGGAGGGTATGCTTCCAATTCCGGCAACAACCAGATTATCACAGAGAAGGAAACAATAGCAGCAAGAACAGCTGAGTGGGGTGTGGTTGTGAAATCATCAGACATTGGAGAAGGAAGCTTCACGGGCGTATCTGGAAGGAAAACTTCATCGGGGAGATTTGATTCGTCTGCAAGGAGTTCTGAGGACTCAAATTATGGGGGTGAGATGGGAAATATTCCTAGGGTATCTAATGAGTTGAAGGCAGCTCTGTCAACACTTCAGCAGACTTTTGTTGTTTCTGACGCCACAAAACCTGATTGCCCAATTATGTATGCTAGTAGTGGCTTCTTTGGGATGACTGGGTACTCTTCGAAGGAGGTCATTGGCAGGAACTGCCGATTTCTTCAGGGACCGGAAACGGACCGGAACGAAGTGGCGAAAATCCGAGACGCAGTCAAGACCGGGAAAAGCTACTGCGGTAGGCTCTTCAACTACAAGAAAGATGGAACACCCTTCTGGAATCTTCTCACCATTACCCCAATCAAAGATGAGAAAGGAAACACCATCAAATTTATTGGAATGCAGGTTGAGGTCAGCAAGTACACAGAAGGAGTGAATGACAGGGCACTGAGGCCGAATGGATTGCCGAAGTCGCTAATCCGCTATGATTCTCGCCAGAAGGAGACGGCGTTAGGCTCCATTACGGAAGTTGTCCAAACAGTAAGACATCCACGTTCTCACATCCAGGATGTGCGTCATGACACTGCCAGCATTCATGAGGAGCAGGACAGCCTCAACGCTGATTATGTTCTGCCTAAATCTGCTGCAACTGCAAGTATGAGTACACCTGGTAGACAAACTCCTCAATCGGATGTGAAAGGCGATAAATTTCGCAGCATGAGCTCTCCTATTGATGCTGGCAAAATATCTCGAAAGTCAGGACGCACTTCCTCTATTGGATTTAAAACACGGTCTCTAAGCTCTGCAAGCAGGCATGAAAAAGAACCTATTATTGAACCGGAGGTTTTGATGACCACAGATATAGAGCCCTCTGACAATTGGGATGGTACTGAAAGAGAAAGGGATATACGCCAAGGAATTGACTTGGCAACCACATTGGAACGCATTGAAAAGAACTTTGTGATTAGTGATCCTAGAATTCCAGATTGCCCCATTATATTTGCATCTGATAGCTTCTTAGAACTGACAGAATATACCCGTGAAGAAATTTTGGGAAGAAATTGTCGTTTTCTCCAGGGACCTGAAACAGATCAAGCTACTGTCGCAAAGATAAGAGATGCCATTAGAGAACAAAGGGAAATTACTGTACAGTTGATCAACTATACAAAGAGTGGAAAAAAGTTTTGGAATTTATTTCATTTGCAGCCCATGCGTGACCAGAAGGGTGAACTTCAATATTTCATTGGTGTCCAACTGGATGGGAGTGGTCATGTGGAACCTCTGCGAAATCGCCTATCAGAGACCACAGAGCTAGAAGGTTCTAAATTGGTCAAAGCTACAGCACACAATGTTGATGAGGCTGTTCGAGAACTTCCTGATGCCAACTTGAAACCAGAAGATTTATGGGCAATTCATTCTCAGCCTGTCTTCCCAAGGCCTCACAAAAGGGAAAATCCTTCTTGGACAGCAATACGGGAGATTACTGCTCGCGGTGAAAAAATTGGTCTACATCATTTTAAGCCCATAAAACCATTGGGATGCGGTGATACTGGAAGTGTACATTTGGTGGAACTACAAGGTACAGGTGAACTGTATGCTATGAAGGCAATGGAGAAGTCAATAATGTTGAACCGTAACAAGGTTCACCGAGCATGCATTGAGAGGGAGATAATTTCGCTACTCGACCATCCTTTTCTTCCCACACTGTACACCTCATTTGAGACTTCCACACACGTTTGTTTGATATCAGACTTTTGCAGTGGTGGAGAGTTATTTGCTTTACTTGACAAGCAGCCGATGAAATTATTTAAGGAGGAATCTGCGAGGTTCTATGCGGCAGAGGTGGTTATTGCATTGGAATATCTTCACTGTCTAGGAATAGTATATCGCGACCTTAAGCCTGAAAATATTTTACTCCATAAGGATGGGCATATTGTCTTAACAGATTTTGATTTGTCGTTTATGACATCATGTAAACCCCTGATTATAAGGCATCAATCGCCTAACAAGAGAAGAAGATCGAGGAGTCAACCACCACCAACATTTGTTGCAGAACCAGTCACACAATCAAATTCATTTGTTGGAACTGAAGAGTACATTGCTCCTGAAATTATTACTGGTGCAGGCCACAGCAGTGCTATTGATTGGTGGGCTCTTGGTATTTTGTTGTATGAGATGCTGTATGGCCGTACACCTTTCAGGGGTAAAAATAGGCAGAGGACGTTCACCAACATCCTGCACAAAGATCTCACGTTTCCAGGCAGTATTCCGGTTAGCCTTGCAGCACGGCAGTTGATAAATGCATTGTTGCAGAGAGACCCAGACGCACGTTTAGGATCCAATACTGGTGCAAATGAAATCAAGCAACATCCTTTCTTCCGTGGAATTAATTGGCCTTTGATTCGTTGCATGAGCCCTCCGCCATTAGAAGCGCCTCTTCAACCTATTGAAAGAGATCCAAAGGCCAAGGATATAACGTGGGAAGATGATGGAGTCCTTGACAAGGTCTTTTCCCCCAGTCTTCCTGTACAGCAAACCTCTCCTTCGGAGCATGAAATTCAAATCTTTCAGGCGGAAAGAAACGGATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000166 GO:0003674 GO:0003676 GO:0003723 GO:0003729 GO:0003824 GO:0004672 GO:0004674 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005773 GO:0005794 GO:0005886 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0006996 GO:0007154 GO:0007165 GO:0007623 GO:0008150 GO:0008152 GO:0009314 GO:0009416 GO:0009605 GO:0009606 GO:0009628 GO:0009637 GO:0009638 GO:0009657 GO:0009658 GO:0009719 GO:0009725 GO:0009735 GO:0009785 GO:0009881 GO:0009882 GO:0009898 GO:0009902 GO:0009903 GO:0009904 GO:0009986 GO:0009987 GO:0010033 GO:0010118 GO:0010119 GO:0010155 GO:0010181 GO:0010359 GO:0010360 GO:0010361 GO:0010362 GO:0012505 GO:0016020 GO:0016043 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019538 GO:0019750 GO:0022898 GO:0023052 GO:0030522 GO:0032409 GO:0032410 GO:0032412 GO:0032413 GO:0032553 GO:0032879 GO:0034762 GO:0034763 GO:0034765 GO:0034766 GO:0035556 GO:0036094 GO:0036211 GO:0038023 GO:0042221 GO:0042802 GO:0043167 GO:0043168 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043269 GO:0043271 GO:0043412 GO:0044070 GO:0044092 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044425 GO:0044444 GO:0044459 GO:0044464 GO:0046777 GO:0048037 GO:0048511 GO:0048519 GO:0050662 GO:0050789 GO:0050794 GO:0050896 GO:0051049 GO:0051051 GO:0051179 GO:0051234 GO:0051640 GO:0051641 GO:0051644 GO:0051649 GO:0051656 GO:0051667 GO:0051716 GO:0060089 GO:0065007 GO:0065009 GO:0071214 GO:0071478 GO:0071482 GO:0071483 GO:0071704 GO:0071840 GO:0071944 GO:0097159 GO:0097367 GO:0098552 GO:0098562 GO:0104004 GO:0140096 GO:1901265 GO:1901363 GO:1901564 GO:1903792 GO:1903959 GO:1903960 GO:1904062
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PAS_9 Pkinase

Protein Analysis

979

Amino Acids

108.96

Weight (kDa)

6.94

Isoelectric Point (pI)

47.35

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PAS PF00989 144 - 250 1.4e-07 PAS fold
PAS_9 PF13426 159 - 255 4.6e-19 PAS domain
PAS_3 PF08447 165 - 249 1.6e-07 PAS fold
PAS_9 PF13426 444 - 536 1.3e-20 PAS domain
PAS_3 PF08447 448 - 533 1.3e-06 PAS fold
PAS PF00989 448 - 536 1.2e-06 PAS fold
Pkinase PF00069 624 - 909 2.6e-59 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 625 - 770 2.1e-14 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 2336
Acc36I ACCTGC 1 cut(s) 742
AccI GTMKAC 2 cut(s) 1021, 1855
AccII CGCG 2 cut(s) 1839, 2238
AciI CCGC 7 cut(s) 645, 829, 1839, 1890, 2183, 2768, 2921
AclWI GGATC 6 cut(s) 82, 152, 1313, 2679, 2692, 2798
AcoI YGGCCR 1 cut(s) 2533
AcsI RAATTY 7 cut(s) 737, 1055, 1326, 1395, 1537, 2419, 2904
AcuI CTGAAG 3 cut(s) 431, 557, 2457
AcyI GRCGYC 1 cut(s) 471
AfiI CCNNNNNNNGG 3 cut(s) 1987, 2549, 2851
AflII CTTAAG 1 cut(s) 2243
AflIII ACRYGT 1 cut(s) 2080
AjnI CCWGG 4 cut(s) 912, 1015, 1422, 2605
AjuI GAANNNNNNNTTGG 2 cut(s) 1516, 1548
AleI CACNNNNGTG 1 cut(s) 2481
Alw21I GWGCWC 1 cut(s) 1073
Alw26I GTCTC 6 cut(s) 609, 845, 1146, 1643, 2063, 2658
AlwI GGATC 6 cut(s) 82, 152, 1313, 2679, 2692, 2798
AlwNI CAGNNNCTG 1 cut(s) 561
AoxI GGCC 6 cut(s) 800, 1784, 2473, 2533, 2741, 2811
ApeKI GCWGC 8 cut(s) 53, 248, 431, 992, 1062, 1552, 2140, 2630
ApoI RAATTY 7 cut(s) 737, 1055, 1326, 1395, 1537, 2419, 2904
AseI ATTAAT 1 cut(s) 2736
Asp700I GAANNNNTTC 3 cut(s) 85, 109, 2211
AspA2I CCTAGG 1 cut(s) 407
AspLEI GCGC 1 cut(s) 2782
AspS9I GGNCC 3 cut(s) 578, 589, 1427
AsuC2I CCSGG 1 cut(s) 631
AsuHPI GGTGA 8 cut(s) 401, 688, 1586, 1853, 1904, 1946, 1985, 2566
AsuII TTCGAA 1 cut(s) 539
AvaII GGWCC 3 cut(s) 578, 589, 1427
AvrII CCTAGG 1 cut(s) 407
BaeGI GKGCMC 1 cut(s) 796
BaeI ACNNNNGTAYC 2 cut(s) 300, 333
BamHI GGATCC 1 cut(s) 2684
BanII GRGCYC 4 cut(s) 1073, 1218, 2503, 2765
BbsI GAAGAC 2 cut(s) 1768, 2861
Bbv12I GWGCWC 1 cut(s) 1073
BbvI GCAGC 8 cut(s) 40, 260, 443, 979, 1074, 1564, 2152, 2642
BceAI ACGGC 3 cut(s) 870, 2520, 2651
BcgI CGANNNNNNTGC 2 cut(s) 793, 827
BciT130I CCWGG 4 cut(s) 914, 1017, 1424, 2607
BclI TGATCA 2 cut(s) 142, 1503
BcnI CCSGG 1 cut(s) 631
BcoDI GTCTC 6 cut(s) 609, 845, 1146, 1643, 2063, 2658
BfaI CTAG 6 cut(s) 39, 408, 504, 1323, 1661, 2222
BfmI CTRYAG 1 cut(s) 1686
BfoI RGCGCY 1 cut(s) 2783
BfrI CTTAAG 1 cut(s) 2243
BfuAI ACCTGC 1 cut(s) 742
BglII AGATCT 1 cut(s) 2593
BisI GCNGC 9 cut(s) 54, 249, 432, 993, 1063, 1553, 2141, 2184, 2631
BlnI CCTAGG 1 cut(s) 407
BlsI GCNGC 9 cut(s) 55, 250, 433, 994, 1064, 1554, 2142, 2185, 2632
Bme1390I CCNGG 5 cut(s) 631, 914, 1017, 1424, 2607
Bme18I GGWCC 3 cut(s) 578, 589, 1427
BmgT120I GGNCC 3 cut(s) 578, 589, 1427
BmiI GGNNCC 5 cut(s) 579, 865, 1428, 1626, 2686
BmrFI CCNGG 5 cut(s) 631, 914, 1017, 1424, 2607
BmrI ACTGGG 2 cut(s) 537, 2859
BmsI GCATC 7 cut(s) 1072, 1361, 1456, 1717, 1877, 2350, 2514
BmuI ACTGGG 2 cut(s) 537, 2859
BpiI GAAGAC 2 cut(s) 1768, 2861
BpmI CTGGAG 2 cut(s) 78, 1406
Bpu14I TTCGAA 1 cut(s) 539
BpuMI CCSGG 1 cut(s) 631
BsaHI GRCGYC 1 cut(s) 471
BsaI GGTCTC 3 cut(s) 1146, 1643, 2658
BsaJI CCNNGG 6 cut(s) 407, 1261, 1423, 1781, 2728, 2814
BsaWI WCCGGW 4 cut(s) 580, 591, 1186, 2617
BsaXI ACNNNNNCTCC 4 cut(s) 76, 106, 842, 872
Bsc4I CCNNNNNNNGG 3 cut(s) 1987, 2549, 2851
Bse1I ACTGG 8 cut(s) 184, 532, 1608, 1903, 2405, 2470, 2698, 2865
Bse3DI GCAATG 2 cut(s) 1962, 2445
BseBI CCWGG 4 cut(s) 914, 1017, 1424, 2607
BseDI CCNNGG 6 cut(s) 407, 1261, 1423, 1781, 2728, 2814
BseLI CCNNNNNNNGG 3 cut(s) 1987, 2549, 2851
BseMI GCAATG 2 cut(s) 1962, 2445
BseMII CTCAG 4 cut(s) 252, 360, 788, 1781
BseNI ACTGG 8 cut(s) 184, 532, 1608, 1903, 2405, 2470, 2698, 2865
BseRI GAGGAG 3 cut(s) 962, 1020, 2386
BseSI GKGCMC 1 cut(s) 796
BseXI GCAGC 8 cut(s) 40, 260, 443, 979, 1074, 1564, 2152, 2642
BsgI GTGCAG 2 cut(s) 2490, 2570
Bsh1236I CGCG 2 cut(s) 1839, 2238
BshFI GGCC 6 cut(s) 802, 1786, 2475, 2535, 2743, 2813
BsiHKAI GWGCWC 1 cut(s) 1073
BsiSI CCGG 6 cut(s) 210, 581, 592, 630, 1187, 2618
BslFI GGGAC 2 cut(s) 591, 1440
BslI CCNNNNNNNGG 3 cut(s) 1987, 2549, 2851
BsmAI GTCTC 6 cut(s) 609, 845, 1146, 1643, 2063, 2658
BsmBI CGTCTC 2 cut(s) 609, 845
BsmFI GGGAC 2 cut(s) 591, 1440
BsmI GAATGC 3 cut(s) 131, 753, 939
BsnI GGCC 6 cut(s) 802, 1786, 2475, 2535, 2743, 2813
Bso31I GGTCTC 3 cut(s) 1146, 1643, 2658
Bsp119I TTCGAA 1 cut(s) 539
Bsp1286I GDGCHC 5 cut(s) 796, 1073, 1218, 2503, 2765
Bsp1407I TGTACA 4 cut(s) 1495, 1906, 2055, 2875
Bsp68I TCGCGA 1 cut(s) 2238
BspACI CCGC 7 cut(s) 645, 829, 1839, 1890, 2183, 2768, 2921
BspANI GGCC 6 cut(s) 802, 1786, 2475, 2535, 2743, 2813
BspCNI CTCAG 4 cut(s) 253, 361, 789, 1780
BspFNI CGCG 2 cut(s) 1839, 2238
BspHI TCATGA 2 cut(s) 925, 943
BspLI GGNNCC 5 cut(s) 579, 865, 1428, 1626, 2686
BspMI ACCTGC 1 cut(s) 742
BspPI GGATC 6 cut(s) 82, 152, 1313, 2679, 2692, 2798
BspQI GCTCTTC 1 cut(s) 659
BspT104I TTCGAA 1 cut(s) 539
BspTI CTTAAG 1 cut(s) 2243
BspTNI GGTCTC 3 cut(s) 1146, 1643, 2658
BsrDI GCAATG 2 cut(s) 1962, 2445
BsrGI TGTACA 4 cut(s) 1495, 1906, 2055, 2875
BsrI ACTGG 8 cut(s) 184, 532, 1608, 1903, 2405, 2470, 2698, 2865
BssECI CCNNGG 6 cut(s) 407, 1261, 1423, 1781, 2728, 2814
BssNI GRCGYC 1 cut(s) 471
BssT1I CCWWGG 4 cut(s) 407, 1261, 1781, 2814
Bst2UI CCWGG 4 cut(s) 914, 1017, 1424, 2607
Bst4CI ACNGT 9 cut(s) 889, 1140, 1450, 1495, 1500, 1941, 1982, 2055, 2219
Bst6I CTCTTC 4 cut(s) 541, 659, 2433, 2790
BstACI GRCGYC 1 cut(s) 471
BstAFI CTTAAG 1 cut(s) 2243
BstAPI GCANNNNNTGC 2 cut(s) 561, 1558
BstAUI TGTACA 4 cut(s) 1495, 1906, 2055, 2875
BstBI TTCGAA 1 cut(s) 539
BstC8I GCNNGC 8 cut(s) 51, 937, 1087, 1156, 1160, 1837, 2002, 2473
BstDEI CTNAG 6 cut(s) 261, 369, 797, 1145, 1366, 1767
BstDSI CCRYGG 1 cut(s) 2728
BstENI CCTNNNNNAGG 1 cut(s) 2849
BstFNI CGCG 2 cut(s) 1839, 2238
BstH2I RGCGCY 1 cut(s) 2783
BstHHI GCGC 1 cut(s) 2782
BstMAI GTCTC 6 cut(s) 609, 845, 1146, 1643, 2063, 2658
BstNI CCWGG 4 cut(s) 914, 1017, 1424, 2607
BstNSI RCATGY 1 cut(s) 2004
BstSCI CCNGG 5 cut(s) 629, 912, 1015, 1422, 2605
BstSFI CTRYAG 1 cut(s) 1686
BstSLI GKGCMC 1 cut(s) 796
BstUI CGCG 2 cut(s) 1839, 2238
BstV1I GCAGC 8 cut(s) 40, 260, 443, 979, 1074, 1564, 2152, 2642
BstV2I GAAGAC 2 cut(s) 1768, 2861
BstX2I RGATCY 3 cut(s) 2593, 2684, 2803
BstXI CCANNNNNNTGG 2 cut(s) 1607, 2273
BstYI RGATCY 3 cut(s) 2593, 2684, 2803
BsuRI GGCC 6 cut(s) 802, 1786, 2475, 2535, 2743, 2813
BtgI CCRYGG 1 cut(s) 2728
BtsI GCAGTG 3 cut(s) 930, 2113, 2488
BtsIMutI CAGTG 7 cut(s) 177, 794, 930, 2051, 2113, 2215, 2488
BtuMI TCGCGA 1 cut(s) 2238
BveI ACCTGC 1 cut(s) 742
Cac8I GCNNGC 8 cut(s) 51, 937, 1087, 1156, 1160, 1837, 2002, 2473
CaiI CAGNNNCTG 1 cut(s) 561
CciI TCATGA 2 cut(s) 925, 943
CfoI GCGC 1 cut(s) 2782
Cfr13I GGNCC 3 cut(s) 578, 589, 1427
CpoI CGGWCCG 1 cut(s) 589
CseI GACGC 5 cut(s) 479, 626, 911, 1119, 2681
CsiI ACCWGGT 1 cut(s) 1015
CspI CGGWCCG 1 cut(s) 589
DdeI CTNAG 6 cut(s) 261, 369, 797, 1145, 1366, 1767
DraI TTTAAA 1 cut(s) 1132
EaeI YGGCCR 1 cut(s) 2533
Eam1104I CTCTTC 4 cut(s) 541, 659, 2433, 2790
EarI CTCTTC 4 cut(s) 541, 659, 2433, 2790
EciI GGCGGA 2 cut(s) 2757, 2936
Ecl136II GAGCTC 1 cut(s) 1071
Eco130I CCWWGG 4 cut(s) 407, 1261, 1781, 2814
Eco147I AGGCCT 1 cut(s) 1786
Eco24I GRGCYC 4 cut(s) 1073, 1218, 2503, 2765
Eco31I GGTCTC 3 cut(s) 1146, 1643, 2658
Eco32I GATATC 1 cut(s) 2094
Eco47I GGWCC 3 cut(s) 578, 589, 1427
Eco53kI GAGCTC 1 cut(s) 1071
Eco57I CTGAAG 3 cut(s) 431, 557, 2457
EcoICRI GAGCTC 1 cut(s) 1071
EcoNI CCTNNNNNAGG 1 cut(s) 2849
EcoO109I RGGNCCY 1 cut(s) 1427
EcoRI GAATTC 1 cut(s) 1326
EcoRII CCWGG 4 cut(s) 912, 1015, 1422, 2605
EcoRV GATATC 1 cut(s) 2094
EcoT14I CCWWGG 4 cut(s) 407, 1261, 1781, 2814
EcoT22I ATGCAT 2 cut(s) 2006, 2653
EcoT38I GRGCYC 4 cut(s) 1073, 1218, 2503, 2765
ErhI CCWWGG 4 cut(s) 407, 1261, 1781, 2814
Esp3I CGTCTC 2 cut(s) 609, 845
FalI AAGNNNNNCTT 4 cut(s) 316, 348, 2769, 2801
FaqI GGGAC 2 cut(s) 591, 1440
FbaI TGATCA 2 cut(s) 142, 1503
FblI GTMKAC 2 cut(s) 1021, 1855
Fnu4HI GCNGC 9 cut(s) 54, 249, 432, 993, 1063, 1553, 2141, 2184, 2631
FriOI GRGCYC 4 cut(s) 1073, 1218, 2503, 2765
Fsp4HI GCNGC 9 cut(s) 54, 249, 432, 993, 1063, 1553, 2141, 2184, 2631
FspBI CTAG 6 cut(s) 39, 408, 504, 1323, 1661, 2222
GlaI GCGC 1 cut(s) 2781
GluI GCNGC 9 cut(s) 54, 249, 432, 993, 1063, 1553, 2141, 2184, 2631
GsuI CTGGAG 2 cut(s) 78, 1406
HaeII RGCGCY 1 cut(s) 2783
HaeIII GGCC 6 cut(s) 802, 1786, 2475, 2535, 2743, 2813
HapII CCGG 6 cut(s) 210, 581, 592, 630, 1187, 2618
HgaI GACGC 5 cut(s) 479, 626, 911, 1119, 2681
HhaI GCGC 1 cut(s) 2782
Hin1I GRCGYC 1 cut(s) 471
Hin6I GCGC 1 cut(s) 2780
HinP1I GCGC 1 cut(s) 2780
HincII GTYRAC 2 cut(s) 441, 2378
HindII GTYRAC 2 cut(s) 441, 2378
HindIII AAGCTT 1 cut(s) 304
HpaII CCGG 6 cut(s) 210, 581, 592, 630, 1187, 2618
HphI GGTGA 8 cut(s) 401, 688, 1586, 1853, 1904, 1946, 1985, 2566
HpyCH4III ACNGT 9 cut(s) 889, 1140, 1450, 1495, 1500, 1941, 1982, 2055, 2219
HpyCH4IV ACGT 6 cut(s) 901, 2082, 2570, 2600, 2677, 2825
HpyF3I CTNAG 6 cut(s) 261, 369, 797, 1145, 1366, 1767
HpySE526I ACGT 6 cut(s) 901, 2082, 2570, 2600, 2677, 2825
Hsp92I GRCGYC 1 cut(s) 471
HspAI GCGC 1 cut(s) 2780
Ksp22I TGATCA 2 cut(s) 142, 1503
LguI GCTCTTC 1 cut(s) 659
LmnI GCTCC 6 cut(s) 29, 97, 869, 949, 2455, 2896
Lsp1109I GCAGC 8 cut(s) 40, 260, 443, 979, 1074, 1564, 2152, 2642
LweI GCATC 7 cut(s) 1072, 1361, 1456, 1717, 1877, 2350, 2514
MabI ACCWGGT 1 cut(s) 1015
MaeI CTAG 6 cut(s) 39, 408, 504, 1323, 1661, 2222
MaeII ACGT 6 cut(s) 901, 2082, 2570, 2600, 2677, 2825
MaeIII GTNAC 3 cut(s) 1562, 1982, 2407
MfeI CAATTG 1 cut(s) 1225
MflI RGATCY 3 cut(s) 2593, 2684, 2803
MhlI GDGCHC 5 cut(s) 796, 1073, 1218, 2503, 2765
MlyI GAGTC 3 cut(s) 368, 2383, 2850
MmeI TCCRAC 3 cut(s) 7, 1624, 2410
Mph1103I ATGCAT 2 cut(s) 2006, 2653
MroXI GAANNNNTTC 3 cut(s) 85, 109, 2211
MseI TTAA 6 cut(s) 1131, 1866, 2157, 2244, 2288, 2736
MslI CAYNNNNRTG 3 cut(s) 1885, 2271, 2481
MspA1I CMGCKG 2 cut(s) 98, 260
MspCI CTTAAG 1 cut(s) 2243
MspI CCGG 6 cut(s) 210, 581, 592, 630, 1187, 2618
MspR9I CCNGG 5 cut(s) 631, 914, 1017, 1424, 2607
MunI CAATTG 1 cut(s) 1225
Mva1269I GAATGC 3 cut(s) 131, 753, 939
MvaI CCWGG 4 cut(s) 914, 1017, 1424, 2607
MvnI CGCG 2 cut(s) 1839, 2238
NciI CCSGG 1 cut(s) 631
NlaIV GGNNCC 5 cut(s) 579, 865, 1428, 1626, 2686
NmuCI GTSAC 2 cut(s) 1562, 2407
NruI TCGCGA 1 cut(s) 2238
NsiI ATGCAT 2 cut(s) 2006, 2653
NspI RCATGY 1 cut(s) 2004
NspV TTCGAA 1 cut(s) 539
OliI CACNNNNGTG 1 cut(s) 2481
PaeI GCATGC 1 cut(s) 2004
PagI TCATGA 2 cut(s) 925, 943
PceI AGGCCT 1 cut(s) 1786
PciSI GCTCTTC 1 cut(s) 659
PctI GAATGC 3 cut(s) 131, 753, 939
PdmI GAANNNNTTC 3 cut(s) 85, 109, 2211
PfeI GAWTC 7 cut(s) 13, 110, 350, 688, 836, 2165, 2749
PflFI GACNNNGTC 2 cut(s) 620, 2852
PfoI TCCNGGA 1 cut(s) 912
PkrI GCNGC 9 cut(s) 55, 250, 433, 994, 1064, 1554, 2142, 2185, 2632
PleI GAGTC 3 cut(s) 368, 2382, 2849
PpsI GAGTC 3 cut(s) 368, 2382, 2849
PpuMI RGGWCCY 1 cut(s) 1427
PshBI ATTAAT 1 cut(s) 2736
PsiI TTATAA 1 cut(s) 2336
Psp124BI GAGCTC 1 cut(s) 1073
Psp5II RGGWCCY 1 cut(s) 1427
Psp6I CCWGG 4 cut(s) 912, 1015, 1422, 2605
PspGI CCWGG 4 cut(s) 912, 1015, 1422, 2605
PspN4I GGNNCC 5 cut(s) 579, 865, 1428, 1626, 2686
PspPI GGNCC 3 cut(s) 578, 589, 1427
PspPPI RGGWCCY 1 cut(s) 1427
PstNI CAGNNNCTG 1 cut(s) 561
PsuI RGATCY 3 cut(s) 2593, 2684, 2803
PsyI GACNNNGTC 2 cut(s) 620, 2852
PvuII CAGCTG 2 cut(s) 98, 260
RruI TCGCGA 1 cut(s) 2238
RseI CAYNNNNRTG 3 cut(s) 1885, 2271, 2481
Rsr2I CGGWCCG 1 cut(s) 589
RsrII CGGWCCG 1 cut(s) 589
SacI GAGCTC 1 cut(s) 1073
SapI GCTCTTC 1 cut(s) 659
SaqAI TTAA 6 cut(s) 1131, 1866, 2157, 2244, 2288, 2736
SatI GCNGC 9 cut(s) 54, 249, 432, 993, 1063, 1553, 2141, 2184, 2631
Sau96I GGNCC 3 cut(s) 578, 589, 1427
SchI GAGTC 3 cut(s) 368, 2383, 2850
ScrFI CCNGG 5 cut(s) 631, 914, 1017, 1424, 2607
SduI GDGCHC 5 cut(s) 796, 1073, 1218, 2503, 2765
SexAI ACCWGGT 1 cut(s) 1015
SfaNI GCATC 7 cut(s) 1072, 1361, 1456, 1717, 1877, 2350, 2514
SfcI CTRYAG 1 cut(s) 1686
SfuI TTCGAA 1 cut(s) 539
SinI GGWCC 3 cut(s) 578, 589, 1427
SmiMI CAYNNNNRTG 3 cut(s) 1885, 2271, 2481
SmlI CTYRAG 1 cut(s) 2243
SmoI CTYRAG 1 cut(s) 2243
SphI GCATGC 1 cut(s) 2004
SseBI AGGCCT 1 cut(s) 1786
SsiI CCGC 7 cut(s) 645, 829, 1839, 1890, 2183, 2768, 2921
SspI AATATT 3 cut(s) 403, 1586, 2257
SspMI CTAG 6 cut(s) 39, 408, 504, 1323, 1661, 2222
SstI GAGCTC 1 cut(s) 1073
StuI AGGCCT 1 cut(s) 1786
StyD4I CCNGG 5 cut(s) 629, 912, 1015, 1422, 2605
StyI CCWWGG 4 cut(s) 407, 1261, 1781, 2814
TaaI ACNGT 9 cut(s) 889, 1140, 1450, 1495, 1500, 1941, 1982, 2055, 2219
TaiI ACGT 6 cut(s) 904, 2085, 2573, 2603, 2680, 2828
TaqI TCGA 5 cut(s) 539, 1099, 1714, 2031, 2369
TatI WGTACW 7 cut(s) 768, 1010, 1495, 1906, 2055, 2442, 2875
TauI GCSGC 1 cut(s) 2186
TfiI GAWTC 7 cut(s) 13, 110, 350, 688, 836, 2165, 2749
Tru1I TTAA 6 cut(s) 1131, 1866, 2157, 2244, 2288, 2736
Tru9I TTAA 6 cut(s) 1131, 1866, 2157, 2244, 2288, 2736
TscAI CASTG 7 cut(s) 184, 801, 937, 2058, 2113, 2222, 2488
TseFI GTSAC 2 cut(s) 1562, 2407
TseI GCWGC 8 cut(s) 53, 248, 431, 992, 1062, 1552, 2140, 2630
Tsp45I GTSAC 2 cut(s) 1562, 2407
TspGWI ACGGA 3 cut(s) 602, 887, 2717
TspRI CASTG 7 cut(s) 184, 801, 937, 2058, 2113, 2222, 2488
Tth111I GACNNNGTC 2 cut(s) 620, 2852
Vha464I CTTAAG 1 cut(s) 2243
VpaK11BI GGWCC 3 cut(s) 578, 589, 1427
VspI ATTAAT 1 cut(s) 2736
XagI CCTNNNNNAGG 1 cut(s) 2849
XapI RAATTY 7 cut(s) 737, 1055, 1326, 1395, 1537, 2419, 2904
XceI RCATGY 1 cut(s) 2004
XcmI CCANNNNNNNNNTGG 2 cut(s) 1749, 1879
XmaJI CCTAGG 1 cut(s) 407
XmiI GTMKAC 2 cut(s) 1021, 1855
XmnI GAANNNNTTC 3 cut(s) 85, 109, 2211
XspI CTAG 6 cut(s) 39, 408, 504, 1323, 1661, 2222
Zsp2I ATGCAT 2 cut(s) 2006, 2653
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.