MD10G1145100.v1.1

Phototropin-2-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0004987
Physical Location & Seq
Reverse (-)
23139154 .. 23150281
11128 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1145100.v1.1.491

Sequence Viewer

Length: 2826 bp
ATGCTCAGTGAAAACTTTCTATCTGGGATATCAAACTCGAATTTTCAGAAGGTGGTGGATTTTCTGTTTTCAATGGAGGTCTTCAACCTGAAAGATGTTGGAGCAAACCCTAGCAACAAGCAAGCTGCTGATACTGTGGAAATTGGACCAGGAAGCAGTTCTTCAACAAAAACTTCCGCTCCTGCTGCCAAAAGGAATTCCATCACCAAGTGGATGGCATTCGAAACTGCTCAAGCTTCAGCCGATCCGAAAAGCACTTTTGCCACTGATTCAAATGAGGGGCATGCTTCCAATTCCGGCAACAACCAGATTATCACAGCTGAGTGGGGGGTTGTGGTGAAGTCATCAGATATTGGGGAACGAAGCTTTAAGGGCATATCTGGAAGAAAAAACTCTTCAGAGAGATTTGAGTCATCTGCAAGGAATTCTGAGGACTCAAATCATGGGGGTGAAATGGGAAATATTCCAAGGGTTTCCAATGAGTTGATGGCAGCTCTGTCTACACTTCAGCAGACTTTTGTTGTTTCTGATGCCACTAAGCCTGATTGCCCAATTGTGTATGCTAGTAGTGGCTTCTTTGGGATGACTGGGTATTCTTCAAAGGAGGTCATTGGCAGGAACTGCCGATTTCTTCAGGGACCGGAAACGGATCGGGATGAAGTGTCGAAAATCCGAGACGCAGTGAAGACCGGGAAAAGTTACTGCGGTAGGCTCTTCAACTACAAGAAAGATGGAACTCCCTTCTGGAATCTTCTCACCATTGCCCCAATCAAAGATGAGCAAGGAAACACCATCAAATTTATTGGAATGCAGGTTGAGGTCAACAAGTACACAGAAGGGGTGAATGACAAGGCACTGAGGGCGAATGGATTGCCCAAGTCACTAATCCGCTCTGATGCTCGTCAGAAGGAAAGCGCGTTAGGCTCCATCATTGAAGTTTTCCAAACAGTTAAACATCCACGTTCTCACATCCGGGTTGCGAGTCATGACACTGTCAGCATTCATGAGGAGCAGGACAGCCTCAACCTTGATTATGTTCTGCCTAAATCCGCTGCAACTGCAAATATGATTACGCCTGGTACACAACCTCCTCAATCGGATGTGAAAGGCGATAAGTTTCACAGCATGAGCTCTCCTATTGATGCGGGAAAAATATCTCGAAAGTCAGGACGTGCTTCCTCCATGGGATTTATAACAAGGTCTCTAAGCTCTGCAAGCAGGCATGAAAAAGAACCTATTATTGAACCGGAGGTTTTGATGACCATAGATATAGAGCCCTCTAACAATTGGGACCGTGCTGAAAGAGAAAGGGATATATGCCAAGGATTTGACTTGGCAACCACATTGGAACGCATTGAAAAGAACTTTGTGATTAGTGATCCTAGAATTCCTGATTGCCCCATTATATTTGCATCTGATAGCTTCTTAGAACTGACAGAATATACCCGTGAAGAAATTTTGGGAAGAAAATGTTGTTTTCTCCAGAGACCTGAAACAGAACAAGCAAGAGAACAAAGGGAAGTTACTGTCCAGTTGATCAACTATACCAAGAGTGGAAAAAAGTTTTTGAATTTATTTCATTTGCAGCCCATGCGTGACCAGCAGGGTGAACTTCAATATTTTATTGGTGTCCAACTGGATGAGAGTGGTCATGTGGAACCTATGGGAAATCGCCTATCAGAGAGCACAGAAATAGAAAGTGCTAAATTGGTCAAAGCTACCGCACACAATGTTGATGAGGCTGTCCGAGAACTTCCTGATGCCAACTTGGTAAGACTAGAAGATTTATGGGCAATTCATTCTCAACCTGTCTGGCCAAGGCCTCACAAAAGGGAGAGTCCTTCTTGGACAGCAATACGGGAGATTACTGCTCGTGGTGAAAAAATTGGTCTACGTCATTTTAAGCCTGTAAAACCATTGGGATGTGGTAATACTGGAAGTGTACATTTGGTGGAACTACAAGGTACAGGTGAACTGTATGCTATGAAGGCAATGGAGAAGTCAAGAATGTTGAACCGCAACAAGGTTCACCGAGCATGCATTGAGAGGGAGATAATTTCACTACTCGACCATCCTTTTCTCCCCACACTTTACACTTCATTTGAGACCTCCACACACGTTTGTTTGATATCAGACTTTTTCAGTGGTGGAGAGTTATTTGCTTTGCTTGACCAGCAGCCAATGAAATTATTTAAGGAGGAATCTGCGAGGTTTTATGCAGCAGAGGTGGTTATCGCCTTGGAATATCTTCACTGTCTAGGAATAGTATATCGCGACCTTAAGCCTGAAAATATTTTACTCCAGAAGGATGGGCATATTGTTTTAACAGATTTTGATTTATCATTTATGACATCGTGTAAACCCCTGATTATAAAGCATCAATCGCTTAACAATAGAAGACAGTTGAGCAGTCAACCACCACCAACATTTATTGCAGAACCAGTCTCACAATCAAATTCATTTGTCGGAACTGAAGATTACATTGCTCCTGAAATTGTTACTGGTGCAGGCCACAGCAGTGCTATTGATTGGTGGGCTCTTGGTATTTTGTTGTATGAGATGCTGTATGGCCGTACACCTTTCAGGGGCAAAAATAGGCAGAGGACATTCACCAACATCCTGCACAAAGATCTCACGTTTCCAGGCAGTATTCCGGTAAGCCTTGCAGCACGGCAGTTGATCAATGCATTGTTGCAAAGAGACCCGGACACACGTTTAGGATCCAATACTGGTGCAAATGAAATCAAGCAACATCCTTTCTTCCGTGGATTTAATTGGCCTTTGATTCGTTGCATGAGCCCTCCGACATTCTCTTCAACCCATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000166 GO:0003674 GO:0003676 GO:0003723 GO:0003729 GO:0003824 GO:0004672 GO:0004674 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005773 GO:0005794 GO:0005886 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0006996 GO:0007154 GO:0007165 GO:0007623 GO:0008150 GO:0008152 GO:0009314 GO:0009416 GO:0009605 GO:0009606 GO:0009628 GO:0009637 GO:0009638 GO:0009657 GO:0009658 GO:0009719 GO:0009725 GO:0009735 GO:0009785 GO:0009881 GO:0009882 GO:0009898 GO:0009902 GO:0009903 GO:0009904 GO:0009986 GO:0009987 GO:0010033 GO:0010118 GO:0010119 GO:0010155 GO:0010181 GO:0010359 GO:0010360 GO:0010361 GO:0010362 GO:0012505 GO:0016020 GO:0016043 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019538 GO:0019750 GO:0022898 GO:0023052 GO:0030522 GO:0032409 GO:0032410 GO:0032412 GO:0032413 GO:0032553 GO:0032879 GO:0034762 GO:0034763 GO:0034765 GO:0034766 GO:0035556 GO:0036094 GO:0036211 GO:0038023 GO:0042221 GO:0042802 GO:0043167 GO:0043168 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043269 GO:0043271 GO:0043412 GO:0044070 GO:0044092 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044425 GO:0044444 GO:0044459 GO:0044464 GO:0046777 GO:0048037 GO:0048511 GO:0048519 GO:0050662 GO:0050789 GO:0050794 GO:0050896 GO:0051049 GO:0051051 GO:0051179 GO:0051234 GO:0051640 GO:0051641 GO:0051644 GO:0051649 GO:0051656 GO:0051667 GO:0051716 GO:0060089 GO:0065007 GO:0065009 GO:0071214 GO:0071478 GO:0071482 GO:0071483 GO:0071704 GO:0071840 GO:0071944 GO:0097159 GO:0097367 GO:0098552 GO:0098562 GO:0104004 GO:0140096 GO:1901265 GO:1901363 GO:1901564 GO:1903792 GO:1903959 GO:1903960 GO:1904062
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PAS_9 Pkinase

Protein Analysis

942

Amino Acids

105.04

Weight (kDa)

6.61

Isoelectric Point (pI)

43.87

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PAS_9 PF13426 179 - 274 8.2e-20 PAS domain
PAS PF00989 185 - 270 1.3e-07 PAS fold
PAS_3 PF08447 185 - 269 3.6e-07 PAS fold
PAS_9 PF13426 464 - 547 1.4e-14 PAS domain
Pkinase PF00069 636 - 921 2.6e-59 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 638 - 783 5.7e-14 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 1193, 2372
Acc36I ACCTGC 1 cut(s) 802
AccBSI CCGCTC 2 cut(s) 179, 891
AccI GTMKAC 2 cut(s) 500, 1891
AccII CGCG 2 cut(s) 917, 2274
AciI CCGC 7 cut(s) 177, 705, 889, 1050, 1145, 1722, 2017
AclWI GGATC 5 cut(s) 239, 657, 1373, 2715, 2728
AcoI YGGCCR 2 cut(s) 1814, 2569
AcsI RAATTY 8 cut(s) 40, 196, 424, 797, 1386, 1455, 1570, 2455
AcuI CTGAAG 5 cut(s) 222, 381, 491, 617, 2493
AdeI CACNNNGTG 1 cut(s) 210
AfaI GTAC 5 cut(s) 830, 1081, 1944, 1966, 2575
AfiI CCNNNNNNNGG 2 cut(s) 2023, 2585
AflII CTTAAG 1 cut(s) 2279
AflIII ACRYGT 2 cut(s) 2116, 2711
AjiI CACGTC 1 cut(s) 1172
AjnI CCWGG 3 cut(s) 148, 1075, 2641
AleI CACNNNNGTG 1 cut(s) 2517
AluBI AGCT 9 cut(s) 125, 236, 320, 366, 494, 1131, 1209, 1422, 1718
AluI AGCT 9 cut(s) 125, 236, 320, 366, 494, 1131, 1209, 1422, 1718
Alw21I GWGCWC 2 cut(s) 1133, 1688
Alw26I GTCTC 6 cut(s) 669, 1206, 1480, 2099, 2449, 2694
AlwI GGATC 5 cut(s) 239, 657, 1373, 2715, 2728
AlwNI CAGNNNCTG 1 cut(s) 621
AoxI GGCC 5 cut(s) 1814, 1820, 2509, 2569, 2777
ApeKI GCWGC 8 cut(s) 125, 185, 491, 1052, 1585, 2176, 2219, 2666
ApoI RAATTY 8 cut(s) 40, 196, 424, 797, 1386, 1455, 1570, 2455
Asp700I GAANNNNTTC 3 cut(s) 15, 157, 2247
AspLEI GCGC 1 cut(s) 917
AspS9I GGNCC 3 cut(s) 146, 638, 1291
AsuC2I CCSGG 3 cut(s) 691, 974, 2705
AsuII TTCGAA 1 cut(s) 222
AvaII GGWCC 3 cut(s) 146, 638, 1291
BalI TGGCCA 1 cut(s) 1816
BamHI GGATCC 1 cut(s) 2720
BanII GRGCYC 4 cut(s) 1133, 1278, 2539, 2801
BauI CACGAG 1 cut(s) 1872
BbsI GAAGAC 3 cut(s) 73, 692, 2404
Bbv12I GWGCWC 2 cut(s) 1133, 1688
BbvI GCAGC 8 cut(s) 112, 172, 503, 1039, 1597, 2188, 2231, 2678
BccI CCATC 8 cut(s) 208, 209, 481, 725, 800, 935, 2079, 2303
BceAI ACGGC 2 cut(s) 2556, 2687
BcgI CGANNNNNNTGC 2 cut(s) 853, 887
BciT130I CCWGG 3 cut(s) 150, 1077, 2643
BclI TGATCA 2 cut(s) 1536, 2679
BcnI CCSGG 3 cut(s) 691, 974, 2705
BcoDI GTCTC 6 cut(s) 669, 1206, 1480, 2099, 2449, 2694
BfaI CTAG 5 cut(s) 111, 564, 1383, 1778, 2258
BfrI CTTAAG 1 cut(s) 2279
BfuAI ACCTGC 1 cut(s) 802
BglII AGATCT 1 cut(s) 2629
BisI GCNGC 8 cut(s) 126, 186, 492, 1053, 1586, 2177, 2220, 2667
BlsI GCNGC 8 cut(s) 127, 187, 493, 1054, 1587, 2178, 2221, 2668
Bme1390I CCNGG 6 cut(s) 150, 691, 974, 1077, 2643, 2705
Bme18I GGWCC 3 cut(s) 146, 638, 1291
BmgBI CACGTC 1 cut(s) 1172
BmgT120I GGNCC 3 cut(s) 146, 638, 1291
BmiI GGNNCC 5 cut(s) 639, 925, 1292, 1659, 2722
BmrFI CCNGG 6 cut(s) 150, 691, 974, 1077, 2643, 2705
BmrI ACTGGG 1 cut(s) 597
BmsI GCATC 7 cut(s) 520, 886, 1132, 1421, 1750, 2386, 2550
BmuI ACTGGG 1 cut(s) 597
BpiI GAAGAC 3 cut(s) 73, 692, 2404
BpmI CTGGAG 2 cut(s) 1466, 2285
Bpu14I TTCGAA 1 cut(s) 222
BpuEI CTTGAG 1 cut(s) 216
BpuMI CCSGG 3 cut(s) 691, 974, 2705
BsaI GGTCTC 4 cut(s) 1206, 1480, 2099, 2694
BsaJI CCNNGG 6 cut(s) 467, 1182, 1321, 1817, 2238, 2764
BsaWI WCCGGW 3 cut(s) 640, 1246, 2653
BsaXI ACNNNNNCTCC 4 cut(s) 163, 193, 1072, 1102
Bsc4I CCNNNNNNNGG 2 cut(s) 2023, 2585
Bse1I ACTGG 7 cut(s) 592, 1531, 1641, 1939, 2441, 2506, 2734
Bse3DI GCAATG 3 cut(s) 759, 1998, 2481
BseBI CCWGG 3 cut(s) 150, 1077, 2643
BseDI CCNNGG 6 cut(s) 467, 1182, 1321, 1817, 2238, 2764
BseLI CCNNNNNNNGG 2 cut(s) 2023, 2585
BseMI GCAATG 3 cut(s) 759, 1998, 2481
BseMII CTCAG 4 cut(s) 19, 312, 420, 848
BseNI ACTGG 7 cut(s) 592, 1531, 1641, 1939, 2441, 2506, 2734
BseRI GAGGAG 2 cut(s) 1022, 1080
BseXI GCAGC 8 cut(s) 112, 172, 503, 1039, 1597, 2188, 2231, 2678
BsgI GTGCAG 2 cut(s) 2526, 2606
Bsh1236I CGCG 2 cut(s) 917, 2274
BshFI GGCC 5 cut(s) 1816, 1822, 2511, 2571, 2779
BsiHKAI GWGCWC 2 cut(s) 1133, 1688
BsiSI CCGG 7 cut(s) 297, 641, 690, 973, 1247, 2654, 2705
BslFI GGGAC 2 cut(s) 651, 1304
BslI CCNNNNNNNGG 2 cut(s) 2023, 2585
BsmAI GTCTC 6 cut(s) 669, 1206, 1480, 2099, 2449, 2694
BsmBI CGTCTC 1 cut(s) 669
BsmFI GGGAC 2 cut(s) 651, 1304
BsmI GAATGC 3 cut(s) 218, 813, 999
BsnI GGCC 5 cut(s) 1816, 1822, 2511, 2571, 2779
Bso31I GGTCTC 4 cut(s) 1206, 1480, 2099, 2694
Bsp119I TTCGAA 1 cut(s) 222
Bsp1286I GDGCHC 5 cut(s) 1133, 1278, 1688, 2539, 2801
Bsp1407I TGTACA 1 cut(s) 1942
Bsp143I GATC 7 cut(s) 244, 649, 1378, 1536, 2629, 2679, 2720
Bsp19I CCATGG 1 cut(s) 1182
Bsp68I TCGCGA 1 cut(s) 2274
BspACI CCGC 7 cut(s) 177, 705, 889, 1050, 1145, 1722, 2017
BspANI GGCC 5 cut(s) 1816, 1822, 2511, 2571, 2779
BspCNI CTCAG 4 cut(s) 18, 313, 421, 849
BspFNI CGCG 2 cut(s) 917, 2274
BspHI TCATGA 2 cut(s) 985, 1003
BspLI GGNNCC 5 cut(s) 639, 925, 1292, 1659, 2722
BspMI ACCTGC 1 cut(s) 802
BspPI GGATC 5 cut(s) 239, 657, 1373, 2715, 2728
BspQI GCTCTTC 1 cut(s) 719
BspT104I TTCGAA 1 cut(s) 222
BspTI CTTAAG 1 cut(s) 2279
BspTNI GGTCTC 4 cut(s) 1206, 1480, 2099, 2694
BsrBI CCGCTC 2 cut(s) 179, 891
BsrDI GCAATG 3 cut(s) 759, 1998, 2481
BsrGI TGTACA 1 cut(s) 1942
BsrI ACTGG 7 cut(s) 592, 1531, 1641, 1939, 2441, 2506, 2734
BssECI CCNNGG 6 cut(s) 467, 1182, 1321, 1817, 2238, 2764
BssMI GATC 7 cut(s) 244, 649, 1378, 1536, 2629, 2679, 2720
BssSI CACGAG 1 cut(s) 1872
BssT1I CCWWGG 5 cut(s) 467, 1182, 1321, 1817, 2238
Bst2BI CACGAG 1 cut(s) 1872
Bst2UI CCWGG 3 cut(s) 150, 1077, 2643
Bst4CI ACNGT 8 cut(s) 136, 949, 994, 1295, 1528, 1977, 2255, 2403
Bst6I CTCTTC 3 cut(s) 400, 719, 2818
BstAFI CTTAAG 1 cut(s) 2279
BstAPI GCANNNNNTGC 2 cut(s) 621, 1591
BstAUI TGTACA 1 cut(s) 1942
BstBI TTCGAA 1 cut(s) 222
BstC8I GCNNGC 6 cut(s) 123, 285, 1216, 1220, 2038, 2509
BstDEI CTNAG 7 cut(s) 5, 321, 429, 537, 857, 1205, 1426
BstDSI CCRYGG 2 cut(s) 1182, 2764
BstFNI CGCG 2 cut(s) 917, 2274
BstHHI GCGC 1 cut(s) 917
BstKTI GATC 7 cut(s) 247, 652, 1381, 1539, 2632, 2682, 2723
BstMAI GTCTC 6 cut(s) 669, 1206, 1480, 2099, 2449, 2694
BstMBI GATC 7 cut(s) 244, 649, 1378, 1536, 2629, 2679, 2720
BstNI CCWGG 3 cut(s) 150, 1077, 2643
BstNSI RCATGY 2 cut(s) 287, 2040
BstSCI CCNGG 6 cut(s) 148, 689, 972, 1075, 2641, 2703
BstUI CGCG 2 cut(s) 917, 2274
BstV1I GCAGC 8 cut(s) 112, 172, 503, 1039, 1597, 2188, 2231, 2678
BstV2I GAAGAC 3 cut(s) 73, 692, 2404
BstX2I RGATCY 2 cut(s) 2629, 2720
BstXI CCANNNNNNTGG 2 cut(s) 214, 2309
BstYI RGATCY 2 cut(s) 2629, 2720
BsuRI GGCC 5 cut(s) 1816, 1822, 2511, 2571, 2779
BtgI CCRYGG 2 cut(s) 1182, 2764
BtrI CACGTC 1 cut(s) 1172
BtsI GCAGTG 2 cut(s) 687, 2524
BtsIMutI CAGTG 8 cut(s) 13, 264, 687, 854, 990, 2149, 2251, 2524
BtuMI TCGCGA 1 cut(s) 2274
BveI ACCTGC 1 cut(s) 802
Cac8I GCNNGC 6 cut(s) 123, 285, 1216, 1220, 2038, 2509
CaiI CAGNNNCTG 1 cut(s) 621
CciI TCATGA 2 cut(s) 985, 1003
CfoI GCGC 1 cut(s) 917
Cfr13I GGNCC 3 cut(s) 146, 638, 1291
CseI GACGC 1 cut(s) 686
Csp6I GTAC 5 cut(s) 829, 1080, 1943, 1965, 2574
CviQI GTAC 5 cut(s) 829, 1080, 1943, 1965, 2574
DdeI CTNAG 7 cut(s) 5, 321, 429, 537, 857, 1205, 1426
DpnI GATC 7 cut(s) 246, 651, 1380, 1538, 2631, 2681, 2722
DpnII GATC 7 cut(s) 244, 649, 1378, 1536, 2629, 2679, 2720
DraIII CACNNNGTG 1 cut(s) 210
EaeI YGGCCR 2 cut(s) 1814, 2569
Eam1104I CTCTTC 3 cut(s) 400, 719, 2818
EarI CTCTTC 3 cut(s) 400, 719, 2818
Ecl136II GAGCTC 1 cut(s) 1131
Eco130I CCWWGG 5 cut(s) 467, 1182, 1321, 1817, 2238
Eco147I AGGCCT 1 cut(s) 1822
Eco24I GRGCYC 4 cut(s) 1133, 1278, 2539, 2801
Eco31I GGTCTC 4 cut(s) 1206, 1480, 2099, 2694
Eco32I GATATC 2 cut(s) 30, 2130
Eco47I GGWCC 3 cut(s) 146, 638, 1291
Eco53kI GAGCTC 1 cut(s) 1131
Eco57I CTGAAG 5 cut(s) 222, 381, 491, 617, 2493
EcoICRI GAGCTC 1 cut(s) 1131
EcoRI GAATTC 3 cut(s) 196, 424, 1386
EcoRII CCWGG 3 cut(s) 148, 1075, 2641
EcoRV GATATC 2 cut(s) 30, 2130
EcoT14I CCWWGG 5 cut(s) 467, 1182, 1321, 1817, 2238
EcoT22I ATGCAT 2 cut(s) 2042, 2689
EcoT38I GRGCYC 4 cut(s) 1133, 1278, 2539, 2801
ErhI CCWWGG 5 cut(s) 467, 1182, 1321, 1817, 2238
Esp3I CGTCTC 1 cut(s) 669
FalI AAGNNNNNCTT 2 cut(s) 145, 177
FaqI GGGAC 2 cut(s) 651, 1304
FauI CCCGC 1 cut(s) 1138
FbaI TGATCA 2 cut(s) 1536, 2679
FblI GTMKAC 2 cut(s) 500, 1891
Fnu4HI GCNGC 8 cut(s) 126, 186, 492, 1053, 1586, 2177, 2220, 2667
FriOI GRGCYC 4 cut(s) 1133, 1278, 2539, 2801
Fsp4HI GCNGC 8 cut(s) 126, 186, 492, 1053, 1586, 2177, 2220, 2667
FspBI CTAG 5 cut(s) 111, 564, 1383, 1778, 2258
GlaI GCGC 1 cut(s) 916
GluI GCNGC 8 cut(s) 126, 186, 492, 1053, 1586, 2177, 2220, 2667
GsuI CTGGAG 2 cut(s) 1466, 2285
HaeIII GGCC 5 cut(s) 1816, 1822, 2511, 2571, 2779
HapII CCGG 7 cut(s) 297, 641, 690, 973, 1247, 2654, 2705
HgaI GACGC 1 cut(s) 686
HhaI GCGC 1 cut(s) 917
Hin6I GCGC 1 cut(s) 915
HinP1I GCGC 1 cut(s) 915
HincII GTYRAC 2 cut(s) 823, 2414
HindII GTYRAC 2 cut(s) 823, 2414
HindIII AAGCTT 2 cut(s) 234, 364
HinfI GANTC 8 cut(s) 269, 410, 434, 748, 982, 1837, 2201, 2785
HpaII CCGG 7 cut(s) 297, 641, 690, 973, 1247, 2654, 2705
HpyAV CCTTC 7 cut(s) 43, 751, 830, 901, 1851, 1981, 2299
HpyCH4III ACNGT 8 cut(s) 136, 949, 994, 1295, 1528, 1977, 2255, 2403
HpyCH4IV ACGT 6 cut(s) 961, 1171, 1894, 2118, 2636, 2713
HpyF3I CTNAG 7 cut(s) 5, 321, 429, 537, 857, 1205, 1426
HpySE526I ACGT 6 cut(s) 961, 1171, 1894, 2118, 2636, 2713
HspAI GCGC 1 cut(s) 915
Ksp22I TGATCA 2 cut(s) 1536, 2679
Kzo9I GATC 7 cut(s) 244, 649, 1378, 1536, 2629, 2679, 2720
LguI GCTCTTC 1 cut(s) 719
LmnI GCTCC 5 cut(s) 101, 184, 929, 1009, 2491
Lsp1109I GCAGC 8 cut(s) 112, 172, 503, 1039, 1597, 2188, 2231, 2678
LweI GCATC 7 cut(s) 520, 886, 1132, 1421, 1750, 2386, 2550
MaeI CTAG 5 cut(s) 111, 564, 1383, 1778, 2258
MaeII ACGT 6 cut(s) 961, 1171, 1894, 2118, 2636, 2713
MaeIII GTNAC 5 cut(s) 698, 879, 1522, 1595, 2497
MalI GATC 7 cut(s) 246, 651, 1380, 1538, 2631, 2681, 2722
MbiI CCGCTC 2 cut(s) 179, 891
MboI GATC 7 cut(s) 244, 649, 1378, 1536, 2629, 2679, 2720
MfeI CAATTG 2 cut(s) 552, 1285
MflI RGATCY 2 cut(s) 2629, 2720
MhlI GDGCHC 5 cut(s) 1133, 1278, 1688, 2539, 2801
MlsI TGGCCA 1 cut(s) 1816
MluNI TGGCCA 1 cut(s) 1816
MlyI GAGTC 4 cut(s) 419, 428, 991, 1846
MmeI TCCRAC 3 cut(s) 79, 1657, 2446
Mox20I TGGCCA 1 cut(s) 1816
Mph1103I ATGCAT 2 cut(s) 2042, 2689
MroXI GAANNNNTTC 3 cut(s) 15, 157, 2247
MscI TGGCCA 1 cut(s) 1816
MseI TTAA 8 cut(s) 369, 951, 1902, 2193, 2280, 2324, 2388, 2772
MslI CAYNNNNRTG 3 cut(s) 447, 1921, 2517
Msp20I TGGCCA 1 cut(s) 1816
MspA1I CMGCKG 2 cut(s) 320, 1052
MspCI CTTAAG 1 cut(s) 2279
MspI CCGG 7 cut(s) 297, 641, 690, 973, 1247, 2654, 2705
MspR9I CCNGG 6 cut(s) 150, 691, 974, 1077, 2643, 2705
MunI CAATTG 2 cut(s) 552, 1285
Mva1269I GAATGC 3 cut(s) 218, 813, 999
MvaI CCWGG 3 cut(s) 150, 1077, 2643
MvnI CGCG 2 cut(s) 917, 2274
NciI CCSGG 3 cut(s) 691, 974, 2705
NcoI CCATGG 1 cut(s) 1182
NdeII GATC 7 cut(s) 244, 649, 1378, 1536, 2629, 2679, 2720
NlaIV GGNNCC 5 cut(s) 639, 925, 1292, 1659, 2722
NmuCI GTSAC 2 cut(s) 879, 1595
NruI TCGCGA 1 cut(s) 2274
NsiI ATGCAT 2 cut(s) 2042, 2689
NspI RCATGY 2 cut(s) 287, 2040
NspV TTCGAA 1 cut(s) 222
OliI CACNNNNGTG 1 cut(s) 2517
PaeI GCATGC 2 cut(s) 287, 2040
PagI TCATGA 2 cut(s) 985, 1003
PceI AGGCCT 1 cut(s) 1822
PciSI GCTCTTC 1 cut(s) 719
PctI GAATGC 3 cut(s) 218, 813, 999
PdmI GAANNNNTTC 3 cut(s) 15, 157, 2247
PfeI GAWTC 4 cut(s) 269, 748, 2201, 2785
PflFI GACNNNGTC 1 cut(s) 992
PkrI GCNGC 8 cut(s) 127, 187, 493, 1054, 1587, 2178, 2221, 2668
PleI GAGTC 4 cut(s) 418, 428, 990, 1845
PpsI GAGTC 4 cut(s) 418, 428, 990, 1845
PsiI TTATAA 2 cut(s) 1193, 2372
Psp124BI GAGCTC 1 cut(s) 1133
Psp6I CCWGG 3 cut(s) 148, 1075, 2641
PspGI CCWGG 3 cut(s) 148, 1075, 2641
PspN4I GGNNCC 5 cut(s) 639, 925, 1292, 1659, 2722
PspPI GGNCC 3 cut(s) 146, 638, 1291
PstNI CAGNNNCTG 1 cut(s) 621
PsuI RGATCY 2 cut(s) 2629, 2720
PsyI GACNNNGTC 1 cut(s) 992
PvuII CAGCTG 1 cut(s) 320
RruI TCGCGA 1 cut(s) 2274
RsaI GTAC 5 cut(s) 830, 1081, 1944, 1966, 2575
RsaNI GTAC 5 cut(s) 829, 1080, 1943, 1965, 2574
RseI CAYNNNNRTG 3 cut(s) 447, 1921, 2517
SacI GAGCTC 1 cut(s) 1133
SapI GCTCTTC 1 cut(s) 719
SaqAI TTAA 8 cut(s) 369, 951, 1902, 2193, 2280, 2324, 2388, 2772
SatI GCNGC 8 cut(s) 126, 186, 492, 1053, 1586, 2177, 2220, 2667
Sau3AI GATC 7 cut(s) 244, 649, 1378, 1536, 2629, 2679, 2720
Sau96I GGNCC 3 cut(s) 146, 638, 1291
SchI GAGTC 4 cut(s) 419, 428, 991, 1846
ScrFI CCNGG 6 cut(s) 150, 691, 974, 1077, 2643, 2705
SduI GDGCHC 5 cut(s) 1133, 1278, 1688, 2539, 2801
SfaNI GCATC 7 cut(s) 520, 886, 1132, 1421, 1750, 2386, 2550
SfuI TTCGAA 1 cut(s) 222
SinI GGWCC 3 cut(s) 146, 638, 1291
SmiMI CAYNNNNRTG 3 cut(s) 447, 1921, 2517
SmlI CTYRAG 2 cut(s) 231, 2279
SmoI CTYRAG 2 cut(s) 231, 2279
SphI GCATGC 2 cut(s) 287, 2040
SseBI AGGCCT 1 cut(s) 1822
SsiI CCGC 7 cut(s) 177, 705, 889, 1050, 1145, 1722, 2017
SspI AATATT 3 cut(s) 463, 1619, 2293
SspMI CTAG 5 cut(s) 111, 564, 1383, 1778, 2258
SstI GAGCTC 1 cut(s) 1133
StuI AGGCCT 1 cut(s) 1822
StyD4I CCNGG 6 cut(s) 148, 689, 972, 1075, 2641, 2703
StyI CCWWGG 5 cut(s) 467, 1182, 1321, 1817, 2238
TaaI ACNGT 8 cut(s) 136, 949, 994, 1295, 1528, 1977, 2255, 2403
TaiI ACGT 6 cut(s) 964, 1174, 1897, 2121, 2639, 2716
TaqI TCGA 5 cut(s) 38, 222, 665, 1159, 2067
TatI WGTACW 2 cut(s) 828, 1942
TfiI GAWTC 4 cut(s) 269, 748, 2201, 2785
Tru1I TTAA 8 cut(s) 369, 951, 1902, 2193, 2280, 2324, 2388, 2772
Tru9I TTAA 8 cut(s) 369, 951, 1902, 2193, 2280, 2324, 2388, 2772
TscAI CASTG 8 cut(s) 13, 271, 687, 861, 997, 2149, 2258, 2524
TseFI GTSAC 2 cut(s) 879, 1595
TseI GCWGC 8 cut(s) 125, 185, 491, 1052, 1585, 2176, 2219, 2666
Tsp45I GTSAC 2 cut(s) 879, 1595
TspGWI ACGGA 2 cut(s) 662, 2753
TspRI CASTG 8 cut(s) 13, 271, 687, 861, 997, 2149, 2258, 2524
Tth111I GACNNNGTC 1 cut(s) 992
Vha464I CTTAAG 1 cut(s) 2279
VpaK11BI GGWCC 3 cut(s) 146, 638, 1291
XapI RAATTY 8 cut(s) 40, 196, 424, 797, 1386, 1455, 1570, 2455
XceI RCATGY 2 cut(s) 287, 2040
XcmI CCANNNNNNNNNTGG 1 cut(s) 484
XmiI GTMKAC 2 cut(s) 500, 1891
XmnI GAANNNNTTC 3 cut(s) 15, 157, 2247
XspI CTAG 5 cut(s) 111, 564, 1383, 1778, 2258
Zsp2I ATGCAT 2 cut(s) 2042, 2689
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.