RchiOBHm_Chr6g0281701

Phototropin-2-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
6
OG0004987
Physical Location & Seq
Reverse (-)
45013150 .. 45023860
10711 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ25266

Sequence Viewer

Length: 2871 bp
ATGGAGAAACCAGAGGCCGCTGGTGGAGATAAGCAGAAATTAGCAGCGCGAAAGAGCAATGATGTTGGAGCACATCCTAGTAGTAAGCTGGAAAGTGGATCATCAAGTACTTCATTTAAGTGGATGGCATTCGATACACAAGCAGCTGAAGAGTCTAACGACACTGCTGCTGCTGCTGCTTCTGGTGACGTTGATGGAATTTTAGACTCAAAAATCATAACGGAGAAGGCGAGCATAGTGGCAAGGACAGCAGAATGGGGAGTTGTGGTGAAGCCGGATGATTCTGGTGAAGGAACTTTTAAGGCCATAGGGAGGTCATCAGATGATGGCAACAAAAGCAAAAACACATCAGGGAGATTTGAATCAAGGACATCATCAGAGTCAAGTCATGGGTCTGATCAGTTGCCTAATCCAAGGGTGTCAAGTGAGTTGAAGACAGCTTTGGCTACATTGCAGCAGACTTTTGTTGTGTCTGATGCCACAAAGCCTGATTGCCCAATCGTGTATGCCAGCAGTGGCTTCTTTACTATGACTGGTTACTCTTCAAAAGAGGTCATTGGAAGAAACTGTCGCTTTCTTCAGGGACCGGAAACAGACCAGAATGAAGTGGCCAAAATCCGAAACGCAGTCAAAACTGGGTCTAGCTACTGCGGTAGGCTCTTCAACTACAAGAAAGATGGCACTCCTTTTTGGAATCTTCTCACCGTCACCCCAATCAAAGATGACAATGGAAAAACCATCAGGTTTATTGGAATGCAGGTTGAGGTCAGCAAGTACACAGAAGGGGTGAATGAGAAGGCATTGAGGCCAAATGGGTTGTCCAAGTCACTAATCCGTTACGACGCTCGACAGAAGGAGAAGGCATTAGGCTCCATTGTTGAAGTTGTACAAACTGTGAAACATCCACGTTCTCACATGCAGGATGCTAGCAATGAGAATACTAAAAATCATGAAGAGCTGGATAACATGAACCTTAATTATGTTCTTCCAAAATCTGCTGCAATGAGTACACCTGTTAGAGAGACTCCTCAGGTGGATGTGAAGGGTGATGCAACTCGGGGGAGATTTTCCCAGGATGCAGGCAAAAATCCTAAAAAGTCAGGGCGCAGCCCCTCAATGGGATTTAGATCAAAGTCTCTAGGCTCTGGAGGCAGGCTTGAAAAAGAACCAAGTGTTGAACCAGAGGATTTGATGACCATAGATATAGAAAATGCTGATAGTTGGGACCGCACAGAAAGAGAAAGGGATATACGCCAAGGAATTGACCTAGCAACCACATTGGAACGCATTGAAAAGAACTTTGTGATTTCTGATCCTAGAATTCCTGATAATCCAATTATATTTGCATCTGATAGTTTTCTAGAACTGACGGAATATACACGTGAGGAAATTCTGGGAAGAAATTGTCGTTTTCTTCAAGGTCCTGAAACAGATCAAGCAACAGTCTCAAAAATAAGAGATGCCATCAGAGAACAACGGGAAATTACTGTACAGTTAATCAACTATACTAAGAGTGGAAAGAAGTTTTGGAATTTGTTCCACTTGCAACCCATGCGTGACCATAAGGGTGAACTTCAATATTTCATTGGTGTCCAATTGGATGGTAGTGATCATGTAGAACCTCTGCGAAATCGCCTGTCAGAGAGAGCAGAGCTAGAAAGTTCTAAAGTGGTGAAAGCCACTGCAGAAAATGTTAACGAGGCTGTCCGAGAACTTCCTGATGCCAACTTGAGACCAGAAGATCTGTGGGCAATTCATTCTCGTCCTGTCTTCCCAAGACCTCACAAAAGGGGTAGTGCTAGTTGGTTAGCAATACAAGAGATTACTTCACGTGGTGAAAAAATTGATCTGCATCATTTTAAGCCCATAAAACCCTTGGGTTGTGGTGATACTGGCAGTGTCCATTTGGTGGAACTACGAGGTACTTCTATACTGTTTGCTATGAAGGCAATGGAGAAGTCAATAATGTTGAATCGTAACAAGGTTCACCGAGCATGCGCTGAACGGGAAATAATTTCACAACTGGATCATCCATTTCTTCCTACGCTATACACTTCATTTGAGACTTCTACACACGTTTGTTTGATAACAGACTTTTGCTCGGGCGGAGAGTTATTTGCTTTGCTTGATAAGCAGCCTATGAAATTCTTCAAGGAGGACTCTGCTAGGTTCTATGCTGCAGAGGTTGTCATTGCCTTGGAATATCTTCACTGTCTAGGTATAATTTATCGCGACCTTAAGCCTGAAAATATTTTACTCCAGAAGGATGGACATATTGTCTTAACAGACTTTGATTTATCATTTATGGCCTCCTGTAAACCACAGATTATAAGGCATCAATCACCTAAGAAGAGAAGGAGATCTAGTAGTCAACCACCACCAACATTTGTTGCAGAACCAGTTAATCAATCAAATTCCTTCGTTGGAACTGAAGAGTACATTGCTCCTGAAATTGTTACCGGTACAGGCCACAGCAGTGCTATTGATTGGTGGGCTCTTGGTATTTTGTTGTATGAGCTGCTATATGGCCGCACCCCTTTCAGGGGGAAAAACAGACAAAGAACATTCACCAACATTTTGCACAAAGATCTGACCTTTCCAAGTAGCATTCCGGTGAGCCTTGCAGCACGGCAGTTGATCAATGCGTTGTTGCAAAGAGACCCTGCCACACGTTTAGGATCCACTACTGGTGCAAACGAAATCAAACAACATCCTTTTTTCCGTGGAATTACTTGGCCTTTGATCCGTTGCATGAGCCCTCCACCGTTAGAGGTGCCTCTTCAGCCTATTGGTTTTGATCCACAGGCCAAGGATATATCGTGGGAAGACGATGGGGTTCTTGTAAATTCAATGGATATGGATATTTTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000166 GO:0003674 GO:0003676 GO:0003723 GO:0003729 GO:0003824 GO:0004672 GO:0004674 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005773 GO:0005794 GO:0005886 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0006996 GO:0007154 GO:0007165 GO:0007623 GO:0008150 GO:0008152 GO:0009314 GO:0009416 GO:0009605 GO:0009606 GO:0009628 GO:0009637 GO:0009638 GO:0009657 GO:0009658 GO:0009719 GO:0009725 GO:0009735 GO:0009785 GO:0009881 GO:0009882 GO:0009898 GO:0009902 GO:0009903 GO:0009904 GO:0009986 GO:0009987 GO:0010033 GO:0010118 GO:0010119 GO:0010155 GO:0010181 GO:0010359 GO:0010360 GO:0010361 GO:0010362 GO:0012505 GO:0016020 GO:0016043 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019538 GO:0019750 GO:0022898 GO:0023052 GO:0030522 GO:0032409 GO:0032410 GO:0032412 GO:0032413 GO:0032553 GO:0032879 GO:0034762 GO:0034763 GO:0034765 GO:0034766 GO:0035556 GO:0036094 GO:0036211 GO:0038023 GO:0042221 GO:0042802 GO:0043167 GO:0043168 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043269 GO:0043271 GO:0043412 GO:0044070 GO:0044092 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044425 GO:0044444 GO:0044459 GO:0044464 GO:0046777 GO:0048037 GO:0048511 GO:0048519 GO:0050662 GO:0050789 GO:0050794 GO:0050896 GO:0051049 GO:0051051 GO:0051179 GO:0051234 GO:0051640 GO:0051641 GO:0051644 GO:0051649 GO:0051656 GO:0051667 GO:0051716 GO:0060089 GO:0065007 GO:0065009 GO:0071214 GO:0071478 GO:0071482 GO:0071483 GO:0071704 GO:0071840 GO:0071944 GO:0097159 GO:0097367 GO:0098552 GO:0098562 GO:0104004 GO:0140096 GO:1901265 GO:1901363 GO:1901564 GO:1903792 GO:1903959 GO:1903960 GO:1904062
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PAS_9 Pkinase

Protein Analysis

956

Amino Acids

106.67

Weight (kDa)

6.77

Isoelectric Point (pI)

42.9

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PAS_9 PF13426 161 - 257 1.3e-20 PAS domain
PAS_3 PF08447 167 - 251 3.4e-09 PAS fold
PAS PF00989 167 - 252 3e-08 PAS fold
PAS_9 PF13426 442 - 534 5.5e-20 PAS domain
PAS_3 PF08447 446 - 531 1.9e-06 PAS fold
PAS PF00989 446 - 534 6.6e-07 PAS fold
Pkinase PF00069 622 - 907 3.6e-60 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 623 - 770 1.3e-16 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 2330
Acc36I ACCTGC 1 cut(s) 748
AccB1I GGYRCC 1 cut(s) 2773
AccB7I CCANNNNNTGG 1 cut(s) 1909
AccII CGCG 2 cut(s) 49, 2232
AciI CCGC 5 cut(s) 18, 651, 1228, 2106, 2530
AclWI GGATC 7 cut(s) 106, 1307, 2034, 2673, 2686, 2737, 2792
AcoI YGGCCR 2 cut(s) 609, 2527
AcsI RAATTY 7 cut(s) 198, 1320, 1389, 1531, 2144, 2413, 2845
AcuI CTGAAG 4 cut(s) 168, 563, 2451, 2765
AcvI CACGTG 2 cut(s) 1382, 1832
AdeI CACNNNGTG 1 cut(s) 1835
AfaI GTAC 8 cut(s) 109, 776, 888, 1009, 1491, 1924, 2438, 2464
AfiI CCNNNNNNNGG 7 cut(s) 312, 1117, 1118, 1909, 2541, 2542, 2543
AflII CTTAAG 1 cut(s) 2237
AflIII ACRYGT 3 cut(s) 1379, 2074, 2669
AgeI ACCGGT 1 cut(s) 2459
AhdI GACNNNNNGTC 1 cut(s) 2276
AjnI CCWGG 1 cut(s) 1071
AjuI GAANNNNNNNTTGG 4 cut(s) 425, 457, 1510, 1542
AleI CACNNNNGTG 1 cut(s) 2475
AluBI AGCT 7 cut(s) 88, 146, 440, 645, 958, 1654, 2518
AluI AGCT 7 cut(s) 88, 146, 440, 645, 958, 1654, 2518
Alw21I GWGCWC 1 cut(s) 73
Alw26I GTCTC 6 cut(s) 1016, 1140, 1450, 1726, 2057, 2652
AlwI GGATC 7 cut(s) 106, 1307, 2034, 2673, 2686, 2737, 2792
Ama87I CYCGRG 2 cut(s) 1056, 2101
AoxI GGCC 9 cut(s) 15, 303, 609, 806, 2307, 2467, 2527, 2735, 2805
ApoI RAATTY 7 cut(s) 198, 1320, 1389, 1531, 2144, 2413, 2845
ArsI GACNNNNNNTTYG 2 cut(s) 803, 835
AsiGI ACCGGT 1 cut(s) 2459
Asp700I GAANNNNTTC 3 cut(s) 1535, 2147, 2205
AspLEI GCGC 3 cut(s) 49, 1107, 2000
AspS9I GGNCC 3 cut(s) 584, 1225, 1421
AsuNHI GCTAGC 1 cut(s) 926
AvaI CYCGRG 2 cut(s) 1056, 2101
AvaII GGWCC 3 cut(s) 584, 1225, 1421
AxyI CCTNAGG 1 cut(s) 1029
BalI TGGCCA 1 cut(s) 611
BamHI GGATCC 1 cut(s) 2678
BanI GGYRCC 1 cut(s) 2773
BanII GRGCYC 2 cut(s) 2497, 2759
BbrPI CACGTG 2 cut(s) 1382, 1832
BbsI GAAGAC 3 cut(s) 440, 1762, 2832
Bbv12I GWGCWC 1 cut(s) 73
BccI CCATC 9 cut(s) 118, 188, 320, 671, 746, 1472, 1595, 2261, 2825
BceAI ACGGC 1 cut(s) 2645
BcgI CGANNNNNNTGC 2 cut(s) 149, 183
BciT130I CCWGG 1 cut(s) 1073
BclI TGATCA 3 cut(s) 397, 1609, 2637
BcoDI GTCTC 6 cut(s) 1016, 1140, 1450, 1726, 2057, 2652
BfmI CTRYAG 2 cut(s) 1683, 2178
BfrI CTTAAG 1 cut(s) 2237
BfuAI ACCTGC 1 cut(s) 748
BglII AGATCT 3 cut(s) 1741, 2360, 2587
BmcAI AGTACT 1 cut(s) 109
Bme1390I CCNGG 1 cut(s) 1073
Bme18I GGWCC 3 cut(s) 584, 1225, 1421
BmeRI GACNNNNNGTC 1 cut(s) 2276
BmeT110I CYCGRG 2 cut(s) 1056, 2101
BmgT120I GGNCC 3 cut(s) 584, 1225, 1421
BmiI GGNNCC 5 cut(s) 585, 871, 1226, 2680, 2775
BmrFI CCNGG 1 cut(s) 1073
BmrI ACTGGG 1 cut(s) 645
BmsI GCATC 9 cut(s) 466, 913, 1039, 1066, 1355, 1450, 1711, 1861, 2344
BmtI GCTAGC 1 cut(s) 930
BmuI ACTGGG 1 cut(s) 645
BpiI GAAGAC 3 cut(s) 440, 1762, 2832
BpmI CTGGAG 2 cut(s) 1167, 2243
BpuEI CTTGAG 1 cut(s) 1750
BsaAI YACGTR 2 cut(s) 1382, 1832
BsaBI GATNNNNATC 3 cut(s) 361, 1126, 1851
BsaI GGTCTC 2 cut(s) 1726, 2652
BsaJI CCNNGG 7 cut(s) 413, 1071, 1255, 1875, 2196, 2722, 2808
BsaWI WCCGGW 3 cut(s) 586, 2459, 2611
BsaXI ACNNNNNCTCC 4 cut(s) 252, 282, 2350, 2380
Bsc4I CCNNNNNNNGG 7 cut(s) 312, 1117, 1118, 1909, 2541, 2542, 2543
Bse118I RCCGGY 1 cut(s) 2459
Bse1I ACTGG 6 cut(s) 538, 640, 1897, 2028, 2399, 2692
Bse21I CCTNAGG 1 cut(s) 1029
Bse3DI GCAATG 7 cut(s) 64, 449, 937, 1008, 1956, 2190, 2439
Bse8I GATNNNNATC 3 cut(s) 361, 1126, 1851
BseBI CCWGG 1 cut(s) 1073
BseDI CCNNGG 7 cut(s) 413, 1071, 1255, 1875, 2196, 2722, 2808
BseJI GATNNNNATC 3 cut(s) 361, 1126, 1851
BseLI CCNNNNNNNGG 7 cut(s) 312, 1117, 1118, 1909, 2541, 2542, 2543
BseMI GCAATG 7 cut(s) 64, 449, 937, 1008, 1956, 2190, 2439
BseMII CTCAG 1 cut(s) 1043
BseNI ACTGG 6 cut(s) 538, 640, 1897, 2028, 2399, 2692
BseRI GAGGAG 1 cut(s) 1017
Bsh1236I CGCG 2 cut(s) 49, 2232
BshFI GGCC 9 cut(s) 17, 305, 611, 808, 2309, 2469, 2529, 2737, 2807
BshNI GGYRCC 1 cut(s) 2773
BshTI ACCGGT 1 cut(s) 2459
BsiHKAI GWGCWC 1 cut(s) 73
BsiHKCI CYCGRG 2 cut(s) 1056, 2101
BsiSI CCGG 4 cut(s) 275, 587, 2460, 2612
BslFI GGGAC 2 cut(s) 597, 1238
BslI CCNNNNNNNGG 7 cut(s) 312, 1117, 1118, 1909, 2541, 2542, 2543
BsmAI GTCTC 6 cut(s) 1016, 1140, 1450, 1726, 2057, 2652
BsmFI GGGAC 2 cut(s) 597, 1238
BsmI GAATGC 3 cut(s) 128, 759, 2607
BsnI GGCC 9 cut(s) 17, 305, 611, 808, 2309, 2469, 2529, 2737, 2807
Bso31I GGTCTC 2 cut(s) 1726, 2652
BsoBI CYCGRG 2 cut(s) 1056, 2101
Bsp1286I GDGCHC 3 cut(s) 73, 2497, 2759
Bsp1407I TGTACA 2 cut(s) 886, 1489
Bsp68I TCGCGA 1 cut(s) 2232
BspACI CCGC 5 cut(s) 18, 651, 1228, 2106, 2530
BspANI GGCC 9 cut(s) 17, 305, 611, 808, 2309, 2469, 2529, 2737, 2807
BspCNI CTCAG 1 cut(s) 1042
BspFNI CGCG 2 cut(s) 49, 2232
BspHI TCATGA 1 cut(s) 949
BspLI GGNNCC 5 cut(s) 585, 871, 1226, 2680, 2775
BspMAI CTGCAG 2 cut(s) 1687, 2182
BspMI ACCTGC 1 cut(s) 748
BspOI GCTAGC 1 cut(s) 930
BspPI GGATC 7 cut(s) 106, 1307, 2034, 2673, 2686, 2737, 2792
BspQI GCTCTTC 2 cut(s) 665, 948
BspT107I GGYRCC 1 cut(s) 2773
BspTI CTTAAG 1 cut(s) 2237
BspTNI GGTCTC 2 cut(s) 1726, 2652
BsrDI GCAATG 7 cut(s) 64, 449, 937, 1008, 1956, 2190, 2439
BsrFI RCCGGY 1 cut(s) 2459
BsrGI TGTACA 2 cut(s) 886, 1489
BsrI ACTGG 6 cut(s) 538, 640, 1897, 2028, 2399, 2692
BssAI RCCGGY 1 cut(s) 2459
BssECI CCNNGG 7 cut(s) 413, 1071, 1255, 1875, 2196, 2722, 2808
BssT1I CCWWGG 5 cut(s) 413, 1255, 1875, 2196, 2808
Bst2UI CCWGG 1 cut(s) 1073
Bst4CI ACNGT 9 cut(s) 569, 706, 895, 1444, 1489, 1494, 1935, 2213, 2766
Bst6I CTCTTC 7 cut(s) 144, 547, 665, 948, 2345, 2427, 2784
BstAFI CTTAAG 1 cut(s) 2237
BstAPI GCANNNNNTGC 1 cut(s) 1552
BstAUI TGTACA 2 cut(s) 886, 1489
BstBAI YACGTR 2 cut(s) 1382, 1832
BstC8I GCNNGC 6 cut(s) 232, 511, 928, 1081, 1154, 1996
BstDEI CTNAG 3 cut(s) 1029, 1509, 2346
BstDSI CCRYGG 1 cut(s) 2722
BstFNI CGCG 2 cut(s) 49, 2232
BstHHI GCGC 3 cut(s) 49, 1107, 2000
BstMAI GTCTC 6 cut(s) 1016, 1140, 1450, 1726, 2057, 2652
BstMWI GCNNNNNNNGC 9 cut(s) 173, 176, 248, 336, 1149, 1552, 1946, 2131, 2782
BstNI CCWGG 1 cut(s) 1073
BstNSI RCATGY 2 cut(s) 919, 1998
BstSCI CCNGG 1 cut(s) 1071
BstSFI CTRYAG 2 cut(s) 1683, 2178
BstUI CGCG 2 cut(s) 49, 2232
BstV2I GAAGAC 3 cut(s) 440, 1762, 2832
BstX2I RGATCY 4 cut(s) 1741, 2360, 2587, 2678
BstXI CCANNNNNNTGG 2 cut(s) 1601, 2267
BstYI RGATCY 4 cut(s) 1741, 2360, 2587, 2678
Bsu36I CCTNAGG 1 cut(s) 1029
BsuRI GGCC 9 cut(s) 17, 305, 611, 808, 2309, 2469, 2529, 2737, 2807
BtgI CCRYGG 1 cut(s) 2722
BtsI GCAGTG 5 cut(s) 162, 520, 1680, 1903, 2482
BtsIMutI CAGTG 6 cut(s) 162, 520, 1680, 1903, 2209, 2482
BtuMI TCGCGA 1 cut(s) 2232
BveI ACCTGC 1 cut(s) 748
Cac8I GCNNGC 6 cut(s) 232, 511, 928, 1081, 1154, 1996
CciI TCATGA 1 cut(s) 949
CfoI GCGC 3 cut(s) 49, 1107, 2000
Cfr10I RCCGGY 1 cut(s) 2459
Cfr13I GGNCC 3 cut(s) 584, 1225, 1421
CseI GACGC 1 cut(s) 851
Csp6I GTAC 8 cut(s) 108, 775, 887, 1008, 1490, 1923, 2437, 2463
CspAI ACCGGT 1 cut(s) 2459
CviAII CATG 8 cut(s) 389, 916, 950, 967, 1552, 1613, 1995, 2752
CviQI GTAC 8 cut(s) 108, 775, 887, 1008, 1490, 1923, 2437, 2463
DdeI CTNAG 3 cut(s) 1029, 1509, 2346
DraIII CACNNNGTG 1 cut(s) 1835
DriI GACNNNNNGTC 1 cut(s) 2276
EaeI YGGCCR 2 cut(s) 609, 2527
Eam1104I CTCTTC 7 cut(s) 144, 547, 665, 948, 2345, 2427, 2784
Eam1105I GACNNNNNGTC 1 cut(s) 2276
EarI CTCTTC 7 cut(s) 144, 547, 665, 948, 2345, 2427, 2784
EciI GGCGGA 1 cut(s) 2121
Eco130I CCWWGG 5 cut(s) 413, 1255, 1875, 2196, 2808
Eco24I GRGCYC 2 cut(s) 2497, 2759
Eco31I GGTCTC 2 cut(s) 1726, 2652
Eco47I GGWCC 3 cut(s) 584, 1225, 1421
Eco57I CTGAAG 4 cut(s) 168, 563, 2451, 2765
Eco72I CACGTG 2 cut(s) 1382, 1832
Eco81I CCTNAGG 1 cut(s) 1029
Eco88I CYCGRG 2 cut(s) 1056, 2101
EcoO109I RGGNCCY 1 cut(s) 1421
EcoRI GAATTC 1 cut(s) 1320
EcoRII CCWGG 1 cut(s) 1071
EcoT14I CCWWGG 5 cut(s) 413, 1255, 1875, 2196, 2808
EcoT38I GRGCYC 2 cut(s) 2497, 2759
ErhI CCWWGG 5 cut(s) 413, 1255, 1875, 2196, 2808
FaeI CATG 8 cut(s) 392, 919, 953, 970, 1555, 1616, 1998, 2755
FaqI GGGAC 2 cut(s) 597, 1238
FatI CATG 8 cut(s) 388, 915, 949, 966, 1551, 1612, 1994, 2751
FbaI TGATCA 3 cut(s) 397, 1609, 2637
FriOI GRGCYC 2 cut(s) 2497, 2759
GlaI GCGC 3 cut(s) 48, 1106, 1999
GsuI CTGGAG 2 cut(s) 1167, 2243
HaeIII GGCC 9 cut(s) 17, 305, 611, 808, 2309, 2469, 2529, 2737, 2807
HapII CCGG 4 cut(s) 275, 587, 2460, 2612
HgaI GACGC 1 cut(s) 851
HhaI GCGC 3 cut(s) 49, 1107, 2000
Hin1II CATG 8 cut(s) 392, 919, 953, 970, 1555, 1616, 1998, 2755
Hin6I GCGC 3 cut(s) 47, 1105, 1998
HinP1I GCGC 3 cut(s) 47, 1105, 1998
HincII GTYRAC 2 cut(s) 1696, 2372
HindII GTYRAC 2 cut(s) 1696, 2372
HinfI GANTC 9 cut(s) 152, 206, 281, 362, 380, 694, 1024, 1972, 2159
HpaI GTTAAC 1 cut(s) 1696
HpaII CCGG 4 cut(s) 275, 587, 2460, 2612
Hpy166II GTNNAC 7 cut(s) 777, 1010, 1571, 1696, 1987, 2318, 2372
Hpy188III TCNNGA 9 cut(s) 950, 1146, 1325, 1361, 1424, 1718, 2231, 2260, 2447
Hpy8I GTNNAC 7 cut(s) 777, 1010, 1571, 1696, 1987, 2318, 2372
Hpy99I CGWCG 1 cut(s) 845
HpyCH4III ACNGT 9 cut(s) 569, 706, 895, 1444, 1489, 1494, 1935, 2213, 2766
HpyCH4IV ACGT 6 cut(s) 189, 907, 1381, 1831, 2076, 2671
HpyF10VI GCNNNNNNNGC 9 cut(s) 173, 176, 248, 336, 1149, 1552, 1946, 2131, 2782
HpyF3I CTNAG 3 cut(s) 1029, 1509, 2346
HpySE526I ACGT 6 cut(s) 189, 907, 1381, 1831, 2076, 2671
Hsp92II CATG 8 cut(s) 392, 919, 953, 970, 1555, 1616, 1998, 2755
HspAI GCGC 3 cut(s) 47, 1105, 1998
Ksp22I TGATCA 3 cut(s) 397, 1609, 2637
KspAI GTTAAC 1 cut(s) 1696
LguI GCTCTTC 2 cut(s) 665, 948
LmnI GCTCC 3 cut(s) 68, 875, 2449
LweI GCATC 9 cut(s) 466, 913, 1039, 1066, 1355, 1450, 1711, 1861, 2344
MaeII ACGT 6 cut(s) 189, 907, 1381, 1831, 2076, 2671
MaeIII GTNAC 8 cut(s) 185, 536, 706, 825, 836, 1556, 1976, 2455
MfeI CAATTG 1 cut(s) 1595
MflI RGATCY 4 cut(s) 1741, 2360, 2587, 2678
MhlI GDGCHC 3 cut(s) 73, 2497, 2759
MlsI TGGCCA 1 cut(s) 611
MluNI TGGCCA 1 cut(s) 611
MlyI GAGTC 5 cut(s) 161, 200, 389, 1018, 2153
MmeI TCCRAC 2 cut(s) 46, 2404
Mox20I TGGCCA 1 cut(s) 611
MroXI GAANNNNTTC 3 cut(s) 1535, 2147, 2205
MscI TGGCCA 1 cut(s) 611
MseI TTAA 9 cut(s) 117, 300, 975, 1496, 1695, 1860, 2238, 2282, 2403
MslI CAYNNNNRTG 4 cut(s) 118, 1566, 2475, 2612
Msp20I TGGCCA 1 cut(s) 611
MspA1I CMGCKG 2 cut(s) 20, 146
MspCI CTTAAG 1 cut(s) 2237
MspI CCGG 4 cut(s) 275, 587, 2460, 2612
MspR9I CCNGG 1 cut(s) 1073
MunI CAATTG 1 cut(s) 1595
Mva1269I GAATGC 3 cut(s) 128, 759, 2607
MvaI CCWGG 1 cut(s) 1073
MvnI CGCG 2 cut(s) 49, 2232
MwoI GCNNNNNNNGC 9 cut(s) 173, 176, 248, 336, 1149, 1552, 1946, 2131, 2782
NheI GCTAGC 1 cut(s) 926
NlaIII CATG 8 cut(s) 392, 919, 953, 970, 1555, 1616, 1998, 2755
NlaIV GGNNCC 5 cut(s) 585, 871, 1226, 2680, 2775
NmuCI GTSAC 4 cut(s) 185, 706, 825, 1556
NruI TCGCGA 1 cut(s) 2232
NspI RCATGY 2 cut(s) 919, 1998
OliI CACNNNNGTG 1 cut(s) 2475
PaeI GCATGC 1 cut(s) 1998
PagI TCATGA 1 cut(s) 949
PciSI GCTCTTC 2 cut(s) 665, 948
PcsI WCGNNNNNNNCGW 1 cut(s) 227
PctI GAATGC 3 cut(s) 128, 759, 2607
PdmI GAANNNNTTC 3 cut(s) 1535, 2147, 2205
PfeI GAWTC 4 cut(s) 281, 362, 694, 1972
PflMI CCANNNNNTGG 1 cut(s) 1909
PinAI ACCGGT 1 cut(s) 2459
PleI GAGTC 5 cut(s) 160, 200, 388, 1018, 2153
PmaCI CACGTG 2 cut(s) 1382, 1832
PmlI CACGTG 2 cut(s) 1382, 1832
PpsI GAGTC 5 cut(s) 160, 200, 388, 1018, 2153
Ppu21I YACGTR 2 cut(s) 1382, 1832
PpuMI RGGWCCY 1 cut(s) 1421
PsiI TTATAA 1 cut(s) 2330
Psp5II RGGWCCY 1 cut(s) 1421
Psp6I CCWGG 1 cut(s) 1071
PspCI CACGTG 2 cut(s) 1382, 1832
PspGI CCWGG 1 cut(s) 1071
PspN4I GGNNCC 5 cut(s) 585, 871, 1226, 2680, 2775
PspPI GGNCC 3 cut(s) 584, 1225, 1421
PspPPI RGGWCCY 1 cut(s) 1421
PstI CTGCAG 2 cut(s) 1687, 2182
PsuI RGATCY 4 cut(s) 1741, 2360, 2587, 2678
PvuII CAGCTG 1 cut(s) 146
RruI TCGCGA 1 cut(s) 2232
RsaI GTAC 8 cut(s) 109, 776, 888, 1009, 1491, 1924, 2438, 2464
RsaNI GTAC 8 cut(s) 108, 775, 887, 1008, 1490, 1923, 2437, 2463
RseI CAYNNNNRTG 4 cut(s) 118, 1566, 2475, 2612
SapI GCTCTTC 2 cut(s) 665, 948
SaqAI TTAA 9 cut(s) 117, 300, 975, 1496, 1695, 1860, 2238, 2282, 2403
Sau96I GGNCC 3 cut(s) 584, 1225, 1421
ScaI AGTACT 1 cut(s) 109
SchI GAGTC 5 cut(s) 161, 200, 389, 1018, 2153
ScrFI CCNGG 1 cut(s) 1073
SduI GDGCHC 3 cut(s) 73, 2497, 2759
SfaNI GCATC 9 cut(s) 466, 913, 1039, 1066, 1355, 1450, 1711, 1861, 2344
SfcI CTRYAG 2 cut(s) 1683, 2178
SinI GGWCC 3 cut(s) 584, 1225, 1421
SmiMI CAYNNNNRTG 4 cut(s) 118, 1566, 2475, 2612
SmlI CTYRAG 2 cut(s) 1729, 2237
SmoI CTYRAG 2 cut(s) 1729, 2237
SphI GCATGC 1 cut(s) 1998
SsiI CCGC 5 cut(s) 18, 651, 1228, 2106, 2530
SspI AATATT 2 cut(s) 1580, 2251
StyD4I CCNGG 1 cut(s) 1071
StyI CCWWGG 5 cut(s) 413, 1255, 1875, 2196, 2808
TaaI ACNGT 9 cut(s) 569, 706, 895, 1444, 1489, 1494, 1935, 2213, 2766
TaiI ACGT 6 cut(s) 192, 910, 1384, 1834, 2079, 2674
TaqI TCGA 2 cut(s) 132, 847
TatI WGTACW 6 cut(s) 107, 774, 886, 1007, 1489, 2436
TauI GCSGC 2 cut(s) 20, 2532
TfiI GAWTC 4 cut(s) 281, 362, 694, 1972
Tru1I TTAA 9 cut(s) 117, 300, 975, 1496, 1695, 1860, 2238, 2282, 2403
Tru9I TTAA 9 cut(s) 117, 300, 975, 1496, 1695, 1860, 2238, 2282, 2403
TscAI CASTG 6 cut(s) 169, 520, 1687, 1903, 2216, 2482
TseFI GTSAC 4 cut(s) 185, 706, 825, 1556
Tsp45I GTSAC 4 cut(s) 185, 706, 825, 1556
TspDTI ATGAA 9 cut(s) 102, 618, 966, 983, 1573, 1745, 1958, 2046, 2156
TspGWI ACGGA 5 cut(s) 236, 824, 1385, 2711, 2735
TspRI CASTG 6 cut(s) 169, 520, 1687, 1903, 2216, 2482
Van91I CCANNNNNTGG 1 cut(s) 1909
Vha464I CTTAAG 1 cut(s) 2237
VpaK11BI GGWCC 3 cut(s) 584, 1225, 1421
XapI RAATTY 7 cut(s) 198, 1320, 1389, 1531, 2144, 2413, 2845
XbaI TCTAGA 1 cut(s) 1360
XceI RCATGY 2 cut(s) 919, 1998
XcmI CCANNNNNNNNNTGG 2 cut(s) 1743, 1873
XmnI GAANNNNTTC 3 cut(s) 1535, 2147, 2205
ZrmI AGTACT 1 cut(s) 109
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.