Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr05

Orthogroup ID

OG0010452

Physical Location & Seq

Genomic Coordinates Reverse (-)

33402565 .. 33405458

Gene Structure 2894 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD05G1204400.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1065 bp

ATGGAGGAAGTTGAAGAAGCTAACAAAGCAGCTGTTGAGAGCTGCCATAGAGTTCTTAGCATCTTGTCTCAGCCACAGGATCAAGTTCAGTATAGGAATCTAATGGTGGAAACTGGAAAGGCAGTGTCTAGGTTTAAGAAAGTTGTCTCCATTCTCAATACTGGTTTAGGTCATGCTAGAGTTAGAAAGCTCAAGAAGCTTCAAATCCCTTTTCCTGAAAGAATCCTTTTGGATAATCCTAACTCGATTGGGGACCGTCCATCCAAAACTCCCCACTTTCTTCAATCTAGATTTCCTGAAAACCCAACTCAAGATTTGAGTTTAGATGTTAAAAGTTCCTTGTGTTTGGGGAATCCATCGTTGGAATTGAGTGCTAATGGGAAAACCCCAATTCATCCTGCCCAACAAGTGCCGCCGGCGCAATATCACTTCTTTCACCAACAACAGCAGCAGCAACATCAACAACAACAGCAACAGCAGCAGAGGATGTTACTTCAGCAGCAGCAGATGAAGCCCCAAACAGAAATGATGTATCGTAGGAGCAACAGCGGCATAAATCTGAATTTTGATAGCTCTAGCTACACACCTACAATGTCATCTACGAGATCCTTCATTTCTTCCTTGAGCATAGATGGAAGTGTGGCTAACTTCGATGGTAATTCCTTCCATTTGATCGGGGCTCCTCTCTCTTCGGATCAGAATTCACATCACAAGAGGAAGTGTTCTGCTAGGGGAGACGATGGGAGTGTTAAATGCGTTGGTAGCAGTGGTAGATGTCACTGCTCAAAGAAGAGGAAGCATAGGGTGAAAAGGTCAATTAAGGTGCCTGCTATCAGTAACAAGCTTGCAGATATCCCTCCTGATGATTATTCATGGCGGAAATATGGCCAGAAGCCAATCAAGGGTTCCCCTCACCCTAGGGGATACTATAAATGCAGTAGCATGAGAGGTTGTCCAGCAAGGAAGCATGTCGAGAGGTGCTTGGAAGAACCCTCGATGCTTATTGTTACCTATGAAGGTGAGCATAACCACCCAAGGATACCATCGCAATCCACAACAACATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003676 GO:0003677 GO:0003700 GO:0005488 GO:0005515 GO:0005516 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006082 GO:0006355 GO:0006790 GO:0006807 GO:0008150 GO:0008152 GO:0009889 GO:0009987 GO:0010468 GO:0010556 GO:0016143 GO:0019219 GO:0019222 GO:0019748 GO:0019757 GO:0019760 GO:0031323 GO:0031326 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0043565 GO:0044237 GO:0044281 GO:0044424 GO:0044464 GO:0050789 GO:0050794 GO:0051171 GO:0051252 GO:0060255 GO:0065007 GO:0071704 GO:0080090 GO:0097159 GO:0140110 GO:1901135 GO:1901363 GO:1901564 GO:1901657 GO:1903506 GO:2000112 GO:2001141

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Plant_zn_clust WRKY

Protein Analysis

355

Amino Acids

39.96

Weight (kDa)

9.7

Isoelectric Point (pI)

55.97

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Plant_zn_clust	PF10533	238 - 284	1.1e-17	Plant zinc cluster domain
WRKY	PF03106	288 - 345	3.5e-27	WRKY DNA -binding domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0010452)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G30590
fragaria_vesca	FvH4_2g22300 FvH4_2g22300 FvH4_2g22300 FvH4_2g22300
malus_domestica	MD05G1204400.v1.1 MD10G1191400.v1.1
prunus_persica	Prupe.8G230700_v2.0.a1
pyrus_communis	pycom05g19070 pycom10g16500
rosa_chinensis	RchiOBHm_Chr6g0289301
rosa_laevigata	RLG00000012315
rosa_multiflora	Rmu_sc0001527.1_g000020
rosa_roxburghii	Rroxscaffold_7G00179360
rosa_rugosa	Rorug06G0202500 Rorug06G0202600
rosa_samantha	Rh6AG313400 Rh6BG320900 Rh6CG327100 Rh6DG312800
rosa_wichuraiana	Rw6G027090

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB1I	GGYRCC	1 cut(s)	823
AciI	CCGC	3 cut(s)	413, 549, 877
AclWI	GGATC	3 cut(s)	87, 600, 702
AcoI	YGGCCR	1 cut(s)	886
AcsI	RAATTY	2 cut(s)	562, 700
AcuI	CTGAAG	1 cut(s)	479
AfiI	CCNNNNNNNGG	1 cut(s)	902
AgsI	TTSAA	3 cut(s)	14, 203, 284
AjuI	GAANNNNNNNTTGG	4 cut(s)	344, 376, 889, 921
AluBI	AGCT	8 cut(s)	20, 32, 42, 190, 199, 573, 579, 844
AluI	AGCT	8 cut(s)	20, 32, 42, 190, 199, 573, 579, 844
Alw26I	GTCTC	3 cut(s)	72, 151, 729
AlwI	GGATC	3 cut(s)	87, 600, 702
AoxI	GGCC	1 cut(s)	886
ApeKI	GCWGC	7 cut(s)	29, 42, 448, 451, 478, 499, 502
ApoI	RAATTY	2 cut(s)	562, 700
AspA2I	CCTAGG	1 cut(s)	917
AspLEI	GCGC	1 cut(s)	421
AspS9I	GGNCC	1 cut(s)	253
AsuHPI	GGTGA	4 cut(s)	428, 817, 905, 1031
AvaII	GGWCC	1 cut(s)	253
AvrII	CCTAGG	1 cut(s)	917
BalI	TGGCCA	1 cut(s)	888
BanI	GGYRCC	1 cut(s)	823
BanII	GRGCYC	1 cut(s)	682
BbvI	GCAGC	7 cut(s)	29, 41, 460, 463, 490, 511, 514
BccI	CCATC	6 cut(s)	268, 364, 626, 647, 734, 1051
BciVI	GTATCC	2 cut(s)	917, 1032
BcoDI	GTCTC	3 cut(s)	72, 151, 729
BfaI	CTAG	6 cut(s)	129, 177, 288, 576, 729, 918
BfuI	GTATCC	2 cut(s)	917, 1032
BisI	GCNGC	9 cut(s)	30, 43, 413, 449, 452, 479, 500, 503, 550
BlnI	CCTAGG	1 cut(s)	917
BlsI	GCNGC	9 cut(s)	31, 44, 414, 450, 453, 480, 501, 504, 551
Bme18I	GGWCC	1 cut(s)	253
BmgT120I	GGNCC	1 cut(s)	253
BmiI	GGNNCC	4 cut(s)	254, 681, 825, 907
BmsI	GCATC	2 cut(s)	69, 987
BpuEI	CTTGAG	3 cut(s)	176, 294, 643
BsaJI	CCNNGG	2 cut(s)	917, 1034
Bsc4I	CCNNNNNNNGG	1 cut(s)	902
Bse118I	RCCGGY	1 cut(s)	415
Bse1I	ACTGG	2 cut(s)	118, 166
BseDI	CCNNGG	2 cut(s)	917, 1034
BseGI	GGATG	3 cut(s)	260, 394, 492
BseLI	CCNNNNNNNGG	1 cut(s)	902
BseMII	CTCAG	1 cut(s)	83
BseNI	ACTGG	2 cut(s)	118, 166
BseRI	GAGGAG	1 cut(s)	672
BseXI	GCAGC	7 cut(s)	29, 41, 460, 463, 490, 511, 514
BshFI	GGCC	1 cut(s)	888
BshNI	GGYRCC	1 cut(s)	823
BsiSI	CCGG	1 cut(s)	416
BslFI	GGGAC	1 cut(s)	266
BslI	CCNNNNNNNGG	1 cut(s)	902
BsmAI	GTCTC	3 cut(s)	72, 151, 729
BsmBI	CGTCTC	1 cut(s)	729
BsmFI	GGGAC	1 cut(s)	266
BsnI	GGCC	1 cut(s)	888
Bsp1286I	GDGCHC	1 cut(s)	682
Bsp143I	GATC	4 cut(s)	79, 605, 672, 694
BspACI	CCGC	3 cut(s)	413, 549, 877
BspANI	GGCC	1 cut(s)	888
BspCNI	CTCAG	1 cut(s)	82
BspLI	GGNNCC	4 cut(s)	254, 681, 825, 907
BspPI	GGATC	3 cut(s)	87, 600, 702
BspT107I	GGYRCC	1 cut(s)	823
BsrFI	RCCGGY	1 cut(s)	415
BsrI	ACTGG	2 cut(s)	118, 166
BssAI	RCCGGY	1 cut(s)	415
BssECI	CCNNGG	2 cut(s)	917, 1034
BssMI	GATC	4 cut(s)	79, 605, 672, 694
BssT1I	CCWWGG	2 cut(s)	917, 1034
Bst4CI	ACNGT	1 cut(s)	257
Bst6I	CTCTTC	2 cut(s)	694, 785
BstC8I	GCNNGC	3 cut(s)	417, 828, 846
BstDEI	CTNAG	2 cut(s)	56, 69
BstF5I	GGATG	3 cut(s)	260, 394, 492
BstHHI	GCGC	1 cut(s)	421
BstKTI	GATC	4 cut(s)	82, 608, 675, 697
BstMAI	GTCTC	3 cut(s)	72, 151, 729
BstMBI	GATC	4 cut(s)	79, 605, 672, 694
BstMWI	GCNNNNNNNGC	7 cut(s)	26, 196, 418, 478, 511, 549, 762
BstNSI	RCATGY	1 cut(s)	971
BstV1I	GCAGC	7 cut(s)	29, 41, 460, 463, 490, 511, 514
BstX2I	RGATCY	1 cut(s)	605
BstYI	RGATCY	1 cut(s)	605
BsuI	GTATCC	2 cut(s)	917, 1032
BsuRI	GGCC	1 cut(s)	888
BtgZI	GCGATG	1 cut(s)	1029
BtsCI	GGATG	3 cut(s)	260, 394, 492
BtsI	GCAGTG	3 cut(s)	129, 772, 778
BtsIMutI	CAGTG	3 cut(s)	129, 772, 778
Cac8I	GCNNGC	3 cut(s)	417, 828, 846
CfoI	GCGC	1 cut(s)	421
Cfr10I	RCCGGY	1 cut(s)	415
Cfr13I	GGNCC	1 cut(s)	253
CviAII	CATG	5 cut(s)	173, 873, 943, 968, 1062
DdeI	CTNAG	2 cut(s)	56, 69
DpnI	GATC	4 cut(s)	81, 607, 674, 696
DpnII	GATC	4 cut(s)	79, 605, 672, 694
EaeI	YGGCCR	1 cut(s)	886
Eam1104I	CTCTTC	2 cut(s)	694, 785
EarI	CTCTTC	2 cut(s)	694, 785
EciI	GGCGGA	1 cut(s)	892
Eco130I	CCWWGG	2 cut(s)	917, 1034
Eco24I	GRGCYC	1 cut(s)	682
Eco32I	GATATC	1 cut(s)	853
Eco47I	GGWCC	1 cut(s)	253
Eco57I	CTGAAG	1 cut(s)	479
EcoRI	GAATTC	1 cut(s)	700
EcoRV	GATATC	1 cut(s)	853
EcoT14I	CCWWGG	2 cut(s)	917, 1034
EcoT38I	GRGCYC	1 cut(s)	682
ErhI	CCWWGG	2 cut(s)	917, 1034
Esp3I	CGTCTC	1 cut(s)	729
FaeI	CATG	5 cut(s)	176, 876, 946, 971, 1065
FaqI	GGGAC	1 cut(s)	266
FatI	CATG	5 cut(s)	172, 872, 942, 967, 1061
Fnu4HI	GCNGC	9 cut(s)	30, 43, 413, 449, 452, 479, 500, 503, 550
FokI	GGATG	3 cut(s)	247, 381, 499
FriOI	GRGCYC	1 cut(s)	682
Fsp4HI	GCNGC	9 cut(s)	30, 43, 413, 449, 452, 479, 500, 503, 550
FspBI	CTAG	6 cut(s)	129, 177, 288, 576, 729, 918
GlaI	GCGC	1 cut(s)	420
GluI	GCNGC	9 cut(s)	30, 43, 413, 449, 452, 479, 500, 503, 550
HaeIII	GGCC	1 cut(s)	888
HapII	CCGG	1 cut(s)	416
HhaI	GCGC	1 cut(s)	421
Hin1II	CATG	5 cut(s)	176, 876, 946, 971, 1065
Hin6I	GCGC	1 cut(s)	419
HinP1I	GCGC	1 cut(s)	419
HindIII	AAGCTT	2 cut(s)	197, 842
HinfI	GANTC	3 cut(s)	97, 222, 352
HpaII	CCGG	1 cut(s)	416
HphI	GGTGA	4 cut(s)	428, 817, 905, 1031
Hpy188I	TCNGA	3 cut(s)	561, 694, 699
Hpy188III	TCNNGA	7 cut(s)	193, 215, 288, 296, 311, 860, 973
HpyAV	CCTTC	3 cut(s)	619, 673, 1010
HpyCH4III	ACNGT	1 cut(s)	257
HpyCH4V	TGCA	2 cut(s)	848, 936
HpyF10VI	GCNNNNNNNGC	7 cut(s)	26, 196, 418, 478, 511, 549, 762
HpyF3I	CTNAG	2 cut(s)	56, 69
Hsp92II	CATG	5 cut(s)	176, 876, 946, 971, 1065
HspAI	GCGC	1 cut(s)	419
KroI	GCCGGC	1 cut(s)	415
KroNI	GCCGGC	1 cut(s)	417
Kzo9I	GATC	4 cut(s)	79, 605, 672, 694
LmnI	GCTCC	2 cut(s)	540, 685
Lsp1109I	GCAGC	7 cut(s)	29, 41, 460, 463, 490, 511, 514
LweI	GCATC	2 cut(s)	69, 987
MaeI	CTAG	6 cut(s)	129, 177, 288, 576, 729, 918
MaeIII	GTNAC	4 cut(s)	489, 776, 836, 1006
MalI	GATC	4 cut(s)	81, 607, 674, 696
MboI	GATC	4 cut(s)	79, 605, 672, 694
MboII	GAAGA	6 cut(s)	26, 272, 609, 681, 802, 998
MflI	RGATCY	1 cut(s)	605
MhlI	GDGCHC	1 cut(s)	682
MlsI	TGGCCA	1 cut(s)	888
MluCI	AATT	6 cut(s)	365, 390, 562, 658, 700, 816
MluNI	TGGCCA	1 cut(s)	888
MmeI	TCCRAC	1 cut(s)	342
MnlI	CCTC	9 cut(s)	477, 693, 708, 786, 867, 921, 941, 969, 1003
Mox20I	TGGCCA	1 cut(s)	888
MreI	CGCCGGCG	1 cut(s)	415
MroNI	GCCGGC	1 cut(s)	415
MscI	TGGCCA	1 cut(s)	888
MseI	TTAA	4 cut(s)	135, 330, 750, 819
Msp20I	TGGCCA	1 cut(s)	888
MspA1I	CMGCKG	2 cut(s)	32, 549
MspI	CCGG	1 cut(s)	416
MwoI	GCNNNNNNNGC	7 cut(s)	26, 196, 418, 478, 511, 549, 762
NaeI	GCCGGC	1 cut(s)	417
NdeII	GATC	4 cut(s)	79, 605, 672, 694
NgoMIV	GCCGGC	1 cut(s)	415
NlaIII	CATG	5 cut(s)	176, 876, 946, 971, 1065
NlaIV	GGNNCC	4 cut(s)	254, 681, 825, 907
NmuCI	GTSAC	1 cut(s)	776
NspI	RCATGY	1 cut(s)	971
PdiI	GCCGGC	1 cut(s)	417
PfeI	GAWTC	3 cut(s)	97, 222, 352
PkrI	GCNGC	9 cut(s)	31, 44, 414, 450, 453, 480, 501, 504, 551
PspN4I	GGNNCC	4 cut(s)	254, 681, 825, 907
PspPI	GGNCC	1 cut(s)	253
PsuI	RGATCY	1 cut(s)	605
PvuII	CAGCTG	1 cut(s)	32
SaqAI	TTAA	4 cut(s)	135, 330, 750, 819
SatI	GCNGC	9 cut(s)	30, 43, 413, 449, 452, 479, 500, 503, 550
Sau3AI	GATC	4 cut(s)	79, 605, 672, 694
Sau96I	GGNCC	1 cut(s)	253
SduI	GDGCHC	1 cut(s)	682
SfaNI	GCATC	2 cut(s)	69, 987
SgrAI	CRCCGGYG	1 cut(s)	415
SinI	GGWCC	1 cut(s)	253
SmlI	CTYRAG	3 cut(s)	191, 309, 622
SmoI	CTYRAG	3 cut(s)	191, 309, 622
Sse9I	AATT	6 cut(s)	365, 390, 562, 658, 700, 816
SsiI	CCGC	3 cut(s)	413, 549, 877
SspMI	CTAG	6 cut(s)	129, 177, 288, 576, 729, 918
StyI	CCWWGG	2 cut(s)	917, 1034
TaaI	ACNGT	1 cut(s)	257
TaqI	TCGA	4 cut(s)	245, 651, 972, 995
TasI	AATT	6 cut(s)	365, 390, 562, 658, 700, 816
TauI	GCSGC	2 cut(s)	415, 552
TfiI	GAWTC	3 cut(s)	97, 222, 352
Tru1I	TTAA	4 cut(s)	135, 330, 750, 819
Tru9I	TTAA	4 cut(s)	135, 330, 750, 819
TscAI	CASTG	3 cut(s)	129, 772, 785
TseFI	GTSAC	1 cut(s)	776
TseI	GCWGC	7 cut(s)	29, 42, 448, 451, 478, 499, 502
Tsp45I	GTSAC	1 cut(s)	776
TspDTI	ATGAA	5 cut(s)	383, 524, 601, 861, 1029
TspRI	CASTG	3 cut(s)	129, 772, 785
VpaK11BI	GGWCC	1 cut(s)	253
XapI	RAATTY	2 cut(s)	562, 700
XbaI	TCTAGA	1 cut(s)	287
XceI	RCATGY	1 cut(s)	971
XmaJI	CCTAGG	1 cut(s)	917
XspI	CTAG	6 cut(s)	129, 177, 288, 576, 729, 918

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.