Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr10

Orthogroup ID

OG0010452

Physical Location & Seq

Genomic Coordinates Reverse (-)

28819078 .. 28822211

Gene Structure 3134 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD10G1191400.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1068 bp

ATGGAGGAAGTTGAAGAAGCTAACAAAACAGCTGTTGAGAGCTGCCATAGAGTTCTTAGCCTATTGTCTCAGCCACAGGAACAAGTTCAGTATAGGAATCTAACGGTGGAAACTGGAAAGGCTGTGTCTAGGTTTAAGAAAGTTGTTTCCATTCTCAATACTGGTTTAGGTCATGCCAGAGTTAGAAAGCTTAAGAAGCTTCAAATCCCTTTTCCTGAAAGAATCCTTTTGGATAATCCTATATCGATTGCGGACCGTCCATCCAAAACTCCCCACTTTCTTCAATCTAGCTTCCCTGAAAACCCAACTCAAGATTTGAGTTTAGATGTTAAAAGTGCGTTGTGTTTGGGGAATCCATCGTTGGAATTGAGTACTAATGGAAAAACCCCAATTCACCCTGCCCAACAAGTGCCGCCCGCTCAATATCACTTGCTTCAGCAACAACGGCAGCACCAGCAACATCAACAACAACAGCAACTGCAGCAGAGGATGTTACTTCAGCAGCACCAGATGAAGCAGCAAACGGAAATGATGTATCGTAGGAGCAATAGTGGCATAAATCTGAATTTTGATAGCTCTAGCTGCACACCTACAATGTCATCTACAAGATCCTTCATTTCTTCCTTGAGCATAGATGGAAGTGTGGCTAACTTCGATGGTAATTCCTTTCATTTGATCGGGGCTCCTCTCTCTTCGGATCAGAATTCACAACATAAGAGGAAGTGTTCTGCTAGGGGAGACGATGGGAGTGTAAAATGCGGTGGTAGCAGTGGTAGATGTCACTGCTCAAAGAAGAGGAAGCATAGGGTTAAAAGGTCAATTAAGGTGCCTGCTATCAGTAACAAGCTTGCAGATATCCCTCCTGATGATTATTCATGGCGGAAATATGGCCAGAAGCCAATCAAGGGTTCCCCTCACCCTAGGGGATACTATAAATGCAGTAGCATGAGAGGTTGTCCAGCAAGGAAGCATGTCGAGAGGTGCTTGGAAGAACCATCCATGCTTATGGTTACCTACGAAGGTGAGCATAACCACCCAAGGATACCATCGCAATCCACAACAACATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003676 GO:0003677 GO:0003700 GO:0005488 GO:0005515 GO:0005516 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006082 GO:0006355 GO:0006790 GO:0006807 GO:0008150 GO:0008152 GO:0009889 GO:0009987 GO:0010468 GO:0010556 GO:0016143 GO:0019219 GO:0019222 GO:0019748 GO:0019757 GO:0019760 GO:0031323 GO:0031326 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0043565 GO:0044237 GO:0044281 GO:0044424 GO:0044464 GO:0050789 GO:0050794 GO:0051171 GO:0051252 GO:0060255 GO:0065007 GO:0071704 GO:0080090 GO:0097159 GO:0140110 GO:1901135 GO:1901363 GO:1901564 GO:1901657 GO:1903506 GO:2000112 GO:2001141

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Plant_zn_clust WRKY

Protein Analysis

356

Amino Acids

39.95

Weight (kDa)

9.68

Isoelectric Point (pI)

58.59

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Plant_zn_clust	PF10533	239 - 285	4.4e-18	Plant zinc cluster domain
WRKY	PF03106	289 - 346	5.1e-27	WRKY DNA -binding domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0010452)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G30590
fragaria_vesca	FvH4_2g22300 FvH4_2g22300 FvH4_2g22300 FvH4_2g22300
malus_domestica	MD05G1204400.v1.1 MD10G1191400.v1.1
prunus_persica	Prupe.8G230700_v2.0.a1
pyrus_communis	pycom05g19070 pycom10g16500
rosa_chinensis	RchiOBHm_Chr6g0289301
rosa_laevigata	RLG00000012315
rosa_multiflora	Rmu_sc0001527.1_g000020
rosa_roxburghii	Rroxscaffold_7G00179360
rosa_rugosa	Rorug06G0202500 Rorug06G0202600
rosa_samantha	Rh6AG313400 Rh6BG320900 Rh6CG327100 Rh6DG312800
rosa_wichuraiana	Rw6G027090

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB1I	GGYRCC	1 cut(s)	826
AccBSI	CCGCTC	1 cut(s)	419
AciI	CCGC	5 cut(s)	251, 413, 417, 759, 880
AclWI	GGATC	2 cut(s)	603, 705
AcoI	YGGCCR	1 cut(s)	889
AcsI	RAATTY	2 cut(s)	565, 703
AcuI	CTGAAG	2 cut(s)	419, 482
AfaI	GTAC	1 cut(s)	373
AfiI	CCNNNNNNNGG	1 cut(s)	905
AflII	CTTAAG	1 cut(s)	191
AgsI	TTSAA	3 cut(s)	14, 203, 284
AjuI	GAANNNNNNNTTGG	4 cut(s)	344, 376, 892, 924
AluBI	AGCT	9 cut(s)	20, 32, 42, 190, 199, 291, 576, 582, 847
AluI	AGCT	9 cut(s)	20, 32, 42, 190, 199, 291, 576, 582, 847
Alw26I	GTCTC	2 cut(s)	72, 732
AlwI	GGATC	2 cut(s)	603, 705
AlwNI	CAGNNNCTG	1 cut(s)	478
AoxI	GGCC	1 cut(s)	889
ApeKI	GCWGC	6 cut(s)	42, 448, 481, 502, 517, 582
ApoI	RAATTY	2 cut(s)	565, 703
Asp700I	GAANNNNTTC	1 cut(s)	84
AspA2I	CCTAGG	1 cut(s)	920
AspS9I	GGNCC	1 cut(s)	253
AsuHPI	GGTGA	3 cut(s)	386, 908, 1034
AvaII	GGWCC	1 cut(s)	253
AvrII	CCTAGG	1 cut(s)	920
BalI	TGGCCA	1 cut(s)	891
BanI	GGYRCC	1 cut(s)	826
BanII	GRGCYC	1 cut(s)	685
BbvI	GCAGC	6 cut(s)	29, 460, 493, 514, 529, 569
BccI	CCATC	7 cut(s)	268, 364, 629, 650, 737, 1003, 1054
BceAI	ACGGC	1 cut(s)	461
BciVI	GTATCC	2 cut(s)	920, 1035
BcoDI	GTCTC	2 cut(s)	72, 732
BfaI	CTAG	5 cut(s)	129, 288, 579, 732, 921
BfmI	CTRYAG	1 cut(s)	479
BfrI	CTTAAG	1 cut(s)	191
BfuI	GTATCC	2 cut(s)	920, 1035
BisI	GCNGC	7 cut(s)	43, 413, 449, 482, 503, 518, 583
BlnI	CCTAGG	1 cut(s)	920
BlsI	GCNGC	7 cut(s)	44, 414, 450, 483, 504, 519, 584
BmcAI	AGTACT	1 cut(s)	373
Bme18I	GGWCC	1 cut(s)	253
BmgT120I	GGNCC	1 cut(s)	253
BmiI	GGNNCC	3 cut(s)	684, 828, 910
BpuEI	CTTGAG	2 cut(s)	294, 646
Bsa29I	ATCGAT	1 cut(s)	245
BsaJI	CCNNGG	2 cut(s)	920, 1037
BsaXI	ACNNNNNCTCC	2 cut(s)	535, 565
Bsc4I	CCNNNNNNNGG	1 cut(s)	905
Bse1I	ACTGG	2 cut(s)	118, 166
BseCI	ATCGAT	1 cut(s)	245
BseDI	CCNNGG	2 cut(s)	920, 1037
BseGI	GGATG	3 cut(s)	260, 495, 995
BseLI	CCNNNNNNNGG	1 cut(s)	905
BseMII	CTCAG	1 cut(s)	83
BseNI	ACTGG	2 cut(s)	118, 166
BseRI	GAGGAG	1 cut(s)	675
BseXI	GCAGC	6 cut(s)	29, 460, 493, 514, 529, 569
BsgI	GTGCAG	1 cut(s)	568
BshFI	GGCC	1 cut(s)	891
BshNI	GGYRCC	1 cut(s)	826
BshVI	ATCGAT	1 cut(s)	245
BslI	CCNNNNNNNGG	1 cut(s)	905
BsmAI	GTCTC	2 cut(s)	72, 732
BsmBI	CGTCTC	1 cut(s)	732
BsnI	GGCC	1 cut(s)	891
Bsp1286I	GDGCHC	1 cut(s)	685
Bsp143I	GATC	3 cut(s)	608, 675, 697
BspACI	CCGC	5 cut(s)	251, 413, 417, 759, 880
BspANI	GGCC	1 cut(s)	891
BspCNI	CTCAG	1 cut(s)	82
BspDI	ATCGAT	1 cut(s)	245
BspLI	GGNNCC	3 cut(s)	684, 828, 910
BspMAI	CTGCAG	1 cut(s)	483
BspPI	GGATC	2 cut(s)	603, 705
BspT107I	GGYRCC	1 cut(s)	826
BspTI	CTTAAG	1 cut(s)	191
BsrBI	CCGCTC	1 cut(s)	419
BsrI	ACTGG	2 cut(s)	118, 166
BssECI	CCNNGG	2 cut(s)	920, 1037
BssMI	GATC	3 cut(s)	608, 675, 697
BssT1I	CCWWGG	2 cut(s)	920, 1037
Bst4CI	ACNGT	2 cut(s)	106, 257
Bst6I	CTCTTC	2 cut(s)	697, 788
BstAFI	CTTAAG	1 cut(s)	191
BstC8I	GCNNGC	3 cut(s)	417, 831, 849
BstDEI	CTNAG	2 cut(s)	56, 69
BstEII	GGTNACC	1 cut(s)	1009
BstF5I	GGATG	3 cut(s)	260, 495, 995
BstKTI	GATC	3 cut(s)	611, 678, 700
BstMAI	GTCTC	2 cut(s)	72, 732
BstMBI	GATC	3 cut(s)	608, 675, 697
BstMWI	GCNNNNNNNGC	7 cut(s)	196, 445, 454, 481, 552, 582, 765
BstNSI	RCATGY	1 cut(s)	974
BstPI	GGTNACC	1 cut(s)	1009
BstSFI	CTRYAG	1 cut(s)	479
BstV1I	GCAGC	6 cut(s)	29, 460, 493, 514, 529, 569
BstX2I	RGATCY	1 cut(s)	608
BstXI	CCANNNNNNTGG	1 cut(s)	1006
BstYI	RGATCY	1 cut(s)	608
Bsu15I	ATCGAT	1 cut(s)	245
BsuI	GTATCC	2 cut(s)	920, 1035
BsuRI	GGCC	1 cut(s)	891
BsuTUI	ATCGAT	1 cut(s)	245
BtgZI	GCGATG	1 cut(s)	1032
BtsCI	GGATG	3 cut(s)	260, 495, 995
BtsI	GCAGTG	2 cut(s)	775, 781
BtsIMutI	CAGTG	2 cut(s)	775, 781
Cac8I	GCNNGC	3 cut(s)	417, 831, 849
CaiI	CAGNNNCTG	1 cut(s)	478
Cfr13I	GGNCC	1 cut(s)	253
ClaI	ATCGAT	1 cut(s)	245
CpoI	CGGWCCG	1 cut(s)	253
Csp6I	GTAC	1 cut(s)	372
CspI	CGGWCCG	1 cut(s)	253
CviAII	CATG	6 cut(s)	173, 876, 946, 971, 1000, 1065
CviQI	GTAC	1 cut(s)	372
DdeI	CTNAG	2 cut(s)	56, 69
DpnI	GATC	3 cut(s)	610, 677, 699
DpnII	GATC	3 cut(s)	608, 675, 697
EaeI	YGGCCR	1 cut(s)	889
Eam1104I	CTCTTC	2 cut(s)	697, 788
EarI	CTCTTC	2 cut(s)	697, 788
EciI	GGCGGA	1 cut(s)	895
Eco130I	CCWWGG	2 cut(s)	920, 1037
Eco24I	GRGCYC	1 cut(s)	685
Eco32I	GATATC	1 cut(s)	856
Eco47I	GGWCC	1 cut(s)	253
Eco57I	CTGAAG	2 cut(s)	419, 482
Eco91I	GGTNACC	1 cut(s)	1009
EcoO65I	GGTNACC	1 cut(s)	1009
EcoRI	GAATTC	1 cut(s)	703
EcoRV	GATATC	1 cut(s)	856
EcoT14I	CCWWGG	2 cut(s)	920, 1037
EcoT38I	GRGCYC	1 cut(s)	685
ErhI	CCWWGG	2 cut(s)	920, 1037
Esp3I	CGTCTC	1 cut(s)	732
FaeI	CATG	6 cut(s)	176, 879, 949, 974, 1003, 1068
FatI	CATG	6 cut(s)	172, 875, 945, 970, 999, 1064
FauI	CCCGC	1 cut(s)	424
Fnu4HI	GCNGC	7 cut(s)	43, 413, 449, 482, 503, 518, 583
FokI	GGATG	3 cut(s)	247, 502, 982
FriOI	GRGCYC	1 cut(s)	685
Fsp4HI	GCNGC	7 cut(s)	43, 413, 449, 482, 503, 518, 583
FspBI	CTAG	5 cut(s)	129, 288, 579, 732, 921
GluI	GCNGC	7 cut(s)	43, 413, 449, 482, 503, 518, 583
HaeIII	GGCC	1 cut(s)	891
Hin1II	CATG	6 cut(s)	176, 879, 949, 974, 1003, 1068
HindIII	AAGCTT	3 cut(s)	188, 197, 845
HinfI	GANTC	3 cut(s)	97, 222, 352
HphI	GGTGA	3 cut(s)	386, 908, 1034
Hpy188I	TCNGA	3 cut(s)	564, 697, 702
Hpy188III	TCNNGA	4 cut(s)	215, 311, 863, 976
HpyAV	CCTTC	2 cut(s)	622, 1013
HpyCH4III	ACNGT	2 cut(s)	106, 257
HpyCH4V	TGCA	4 cut(s)	481, 585, 851, 939
HpyF10VI	GCNNNNNNNGC	7 cut(s)	196, 445, 454, 481, 552, 582, 765
HpyF3I	CTNAG	2 cut(s)	56, 69
Hsp92II	CATG	6 cut(s)	176, 879, 949, 974, 1003, 1068
Kzo9I	GATC	3 cut(s)	608, 675, 697
LmnI	GCTCC	2 cut(s)	543, 688
Lsp1109I	GCAGC	6 cut(s)	29, 460, 493, 514, 529, 569
MaeI	CTAG	5 cut(s)	129, 288, 579, 732, 921
MaeIII	GTNAC	4 cut(s)	492, 779, 839, 1009
MalI	GATC	3 cut(s)	610, 677, 699
MbiI	CCGCTC	1 cut(s)	419
MboI	GATC	3 cut(s)	608, 675, 697
MboII	GAAGA	6 cut(s)	26, 272, 612, 684, 805, 1001
MflI	RGATCY	1 cut(s)	608
MhlI	GDGCHC	1 cut(s)	685
MlsI	TGGCCA	1 cut(s)	891
MluCI	AATT	6 cut(s)	365, 390, 565, 661, 703, 819
MluNI	TGGCCA	1 cut(s)	891
MmeI	TCCRAC	1 cut(s)	342
MnlI	CCTC	8 cut(s)	480, 696, 711, 789, 870, 924, 944, 972
Mox20I	TGGCCA	1 cut(s)	891
MroXI	GAANNNNTTC	1 cut(s)	84
MscI	TGGCCA	1 cut(s)	891
MseI	TTAA	5 cut(s)	135, 192, 330, 810, 822
MslI	CAYNNNNRTG	1 cut(s)	1004
Msp20I	TGGCCA	1 cut(s)	891
MspA1I	CMGCKG	1 cut(s)	32
MspCI	CTTAAG	1 cut(s)	191
MwoI	GCNNNNNNNGC	7 cut(s)	196, 445, 454, 481, 552, 582, 765
NdeII	GATC	3 cut(s)	608, 675, 697
NlaIII	CATG	6 cut(s)	176, 879, 949, 974, 1003, 1068
NlaIV	GGNNCC	3 cut(s)	684, 828, 910
NmuCI	GTSAC	1 cut(s)	779
NspI	RCATGY	1 cut(s)	974
PdmI	GAANNNNTTC	1 cut(s)	84
PfeI	GAWTC	3 cut(s)	97, 222, 352
PkrI	GCNGC	7 cut(s)	44, 414, 450, 483, 504, 519, 584
PspEI	GGTNACC	1 cut(s)	1009
PspN4I	GGNNCC	3 cut(s)	684, 828, 910
PspPI	GGNCC	1 cut(s)	253
PstI	CTGCAG	1 cut(s)	483
PstNI	CAGNNNCTG	1 cut(s)	478
PsuI	RGATCY	1 cut(s)	608
PvuII	CAGCTG	1 cut(s)	32
RsaI	GTAC	1 cut(s)	373
RsaNI	GTAC	1 cut(s)	372
RseI	CAYNNNNRTG	1 cut(s)	1004
Rsr2I	CGGWCCG	1 cut(s)	253
RsrII	CGGWCCG	1 cut(s)	253
SaqAI	TTAA	5 cut(s)	135, 192, 330, 810, 822
SatI	GCNGC	7 cut(s)	43, 413, 449, 482, 503, 518, 583
Sau3AI	GATC	3 cut(s)	608, 675, 697
Sau96I	GGNCC	1 cut(s)	253
ScaI	AGTACT	1 cut(s)	373
SduI	GDGCHC	1 cut(s)	685
SfcI	CTRYAG	1 cut(s)	479
SinI	GGWCC	1 cut(s)	253
SmiMI	CAYNNNNRTG	1 cut(s)	1004
SmlI	CTYRAG	3 cut(s)	191, 309, 625
SmoI	CTYRAG	3 cut(s)	191, 309, 625
Sse9I	AATT	6 cut(s)	365, 390, 565, 661, 703, 819
SsiI	CCGC	5 cut(s)	251, 413, 417, 759, 880
SspMI	CTAG	5 cut(s)	129, 288, 579, 732, 921
StyI	CCWWGG	2 cut(s)	920, 1037
TaaI	ACNGT	2 cut(s)	106, 257
TaqI	TCGA	3 cut(s)	245, 654, 975
TasI	AATT	6 cut(s)	365, 390, 565, 661, 703, 819
TatI	WGTACW	1 cut(s)	371
TauI	GCSGC	1 cut(s)	415
TfiI	GAWTC	3 cut(s)	97, 222, 352
Tru1I	TTAA	5 cut(s)	135, 192, 330, 810, 822
Tru9I	TTAA	5 cut(s)	135, 192, 330, 810, 822
TscAI	CASTG	2 cut(s)	775, 788
TseFI	GTSAC	1 cut(s)	779
TseI	GCWGC	6 cut(s)	42, 448, 481, 502, 517, 582
Tsp45I	GTSAC	1 cut(s)	779
TspDTI	ATGAA	4 cut(s)	527, 604, 659, 864
TspGWI	ACGGA	1 cut(s)	539
TspRI	CASTG	2 cut(s)	775, 788
Vha464I	CTTAAG	1 cut(s)	191
VpaK11BI	GGWCC	1 cut(s)	253
XapI	RAATTY	2 cut(s)	565, 703
XceI	RCATGY	1 cut(s)	974
XmaJI	CCTAGG	1 cut(s)	920
XmnI	GAANNNNTTC	1 cut(s)	84
XspI	CTAG	5 cut(s)	129, 288, 579, 732, 921
ZrmI	AGTACT	1 cut(s)	373

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.