MD05G1216100.v1.1

Snapin/Pallidin

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0009347
Physical Location & Seq
Reverse (-)
34723439 .. 34726058
2620 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1216100.v1.1.491

Sequence Viewer

Length: 441 bp
ATGGAAAGCCAGAAAACCGAGCTGGAGTCATCAGAGGAGAAGAGTGTCACTGTCGACCAGAACGAGAACGCGAAGAACAGATTACAGTCGACGGAGGCGCTGGCAAAGGCGCTTTCGTCGACGCTCACCACCGTCATCAAAGACTTCGATTCCAAAGCTCAGCAGACCTTCGCCAGCCAGGACCACCTCTCTCAGTCGCTCGATCGCCTCACCCGCGAGCTGGATAAGTTGTTGGACGATGCTCCGTTTCTGTTCATCATGCAACACGCGGCGAAGATTTCCGCCGTTAGGAAGAGAGTTTCGTCGTTGAATTCGGTTCTCAAGTCCGTACAGCGCCGGCTTGATAACATTGACAGAATGCTGTTTGTTGGCGTCCGAATCCAGCATCAGCAGCAACAACAACATGATAAGACGGCCACAGGACATTCAGGACTGCCGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Snapin_Pallidin

Protein Analysis

147

Amino Acids

16.46

Weight (kDa)

8.06

Isoelectric Point (pI)

50.65

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Snapin_Pallidin PF14712 32 - 117 6.6e-22 Snapin/Pallidin
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 3 cut(s) 54, 89, 119
AccII CGCG 3 cut(s) 71, 216, 269
AciI CCGC 3 cut(s) 214, 269, 282
AcoI YGGCCR 1 cut(s) 414
AcsI RAATTY 1 cut(s) 310
AcyI GRCGYC 1 cut(s) 372
AfaI GTAC 1 cut(s) 330
AfiI CCNNNNNNNGG 2 cut(s) 220, 288
AgsI TTSAA 1 cut(s) 310
AjnI CCWGG 1 cut(s) 177
AluBI AGCT 3 cut(s) 22, 158, 220
AluI AGCT 3 cut(s) 22, 158, 220
AoxI GGCC 1 cut(s) 414
ApeKI GCWGC 1 cut(s) 391
ApoI RAATTY 1 cut(s) 310
AspLEI GCGC 3 cut(s) 100, 112, 336
AspS9I GGNCC 1 cut(s) 181
AsuHPI GGTGA 2 cut(s) 118, 202
AvaII GGWCC 1 cut(s) 181
BbvI GCAGC 1 cut(s) 403
BceAI ACGGC 3 cut(s) 269, 421, 429
BciT130I CCWGG 1 cut(s) 179
BfoI RGCGCY 3 cut(s) 101, 113, 337
BisI GCNGC 2 cut(s) 270, 392
BlpI GCTNAGC 1 cut(s) 159
BlsI GCNGC 2 cut(s) 271, 393
Bme1390I CCNGG 1 cut(s) 179
Bme18I GGWCC 1 cut(s) 181
BmgT120I GGNCC 1 cut(s) 181
BmrFI CCNGG 1 cut(s) 179
BmsI GCATC 2 cut(s) 229, 394
BpmI CTGGAG 1 cut(s) 44
Bpu1102I GCTNAGC 1 cut(s) 159
BpuEI CTTGAG 1 cut(s) 305
BsaHI GRCGYC 1 cut(s) 372
Bsc4I CCNNNNNNNGG 2 cut(s) 220, 288
Bse118I RCCGGY 1 cut(s) 336
BseBI CCWGG 1 cut(s) 179
BseLI CCNNNNNNNGG 2 cut(s) 220, 288
BseMII CTCAG 2 cut(s) 173, 206
BseRI GAGGAG 1 cut(s) 50
BseXI GCAGC 1 cut(s) 403
Bsh1236I CGCG 3 cut(s) 71, 216, 269
Bsh1285I CGRYCG 1 cut(s) 205
BshFI GGCC 1 cut(s) 416
BsiEI CGRYCG 1 cut(s) 205
BsiSI CCGG 1 cut(s) 337
BslI CCNNNNNNNGG 2 cut(s) 220, 288
BsmI GAATGC 1 cut(s) 363
BsnI GGCC 1 cut(s) 416
Bsp143I GATC 1 cut(s) 202
Bsp1720I GCTNAGC 1 cut(s) 159
BspACI CCGC 3 cut(s) 214, 269, 282
BspANI GGCC 1 cut(s) 416
BspCNI CTCAG 2 cut(s) 172, 205
BspFNI CGCG 3 cut(s) 71, 216, 269
BsrFI RCCGGY 1 cut(s) 336
BssAI RCCGGY 1 cut(s) 336
BssMI GATC 1 cut(s) 202
BssNI GRCGYC 1 cut(s) 372
Bst2UI CCWGG 1 cut(s) 179
Bst4CI ACNGT 3 cut(s) 52, 87, 133
Bst6I CTCTTC 2 cut(s) 35, 287
BstACI GRCGYC 1 cut(s) 372
BstC8I GCNNGC 4 cut(s) 102, 175, 218, 338
BstDEI CTNAG 2 cut(s) 159, 192
BstFNI CGCG 3 cut(s) 71, 216, 269
BstH2I RGCGCY 3 cut(s) 101, 113, 337
BstHHI GCGC 3 cut(s) 100, 112, 336
BstKTI GATC 1 cut(s) 205
BstMBI GATC 1 cut(s) 202
BstMCI CGRYCG 1 cut(s) 205
BstMWI GCNNNNNNNGC 2 cut(s) 213, 391
BstNI CCWGG 1 cut(s) 179
BstSCI CCNGG 1 cut(s) 177
BstUI CGCG 3 cut(s) 71, 216, 269
BstV1I GCAGC 1 cut(s) 403
BsuRI GGCC 1 cut(s) 416
BtsIMutI CAGTG 1 cut(s) 48
Cac8I GCNNGC 4 cut(s) 102, 175, 218, 338
CfoI GCGC 3 cut(s) 100, 112, 336
Cfr10I RCCGGY 1 cut(s) 336
Cfr13I GGNCC 1 cut(s) 181
CseI GACGC 2 cut(s) 130, 361
Csp6I GTAC 1 cut(s) 329
CviAII CATG 2 cut(s) 259, 404
CviJI RGCY 7 cut(s) 9, 22, 158, 177, 220, 340, 416
CviKI_1 RGCY 7 cut(s) 9, 22, 158, 177, 220, 340, 416
CviQI GTAC 1 cut(s) 329
DdeI CTNAG 2 cut(s) 159, 192
DpnI GATC 1 cut(s) 204
DpnII GATC 1 cut(s) 202
EaeI YGGCCR 1 cut(s) 414
Eam1104I CTCTTC 2 cut(s) 35, 287
EarI CTCTTC 2 cut(s) 35, 287
EciI GGCGGA 1 cut(s) 271
Eco47I GGWCC 1 cut(s) 181
EcoRI GAATTC 1 cut(s) 310
EcoRII CCWGG 1 cut(s) 177
FaeI CATG 2 cut(s) 262, 407
FaiI YATR 2 cut(s) 260, 405
FatI CATG 2 cut(s) 258, 403
FauI CCCGC 1 cut(s) 221
FblI GTMKAC 3 cut(s) 54, 89, 119
Fnu4HI GCNGC 2 cut(s) 270, 392
Fsp4HI GCNGC 2 cut(s) 270, 392
GlaI GCGC 3 cut(s) 99, 111, 335
GluI GCNGC 2 cut(s) 270, 392
GsuI CTGGAG 1 cut(s) 44
HaeII RGCGCY 3 cut(s) 101, 113, 337
HaeIII GGCC 1 cut(s) 416
HapII CCGG 1 cut(s) 337
HgaI GACGC 2 cut(s) 130, 361
HhaI GCGC 3 cut(s) 100, 112, 336
Hin1I GRCGYC 1 cut(s) 372
Hin1II CATG 2 cut(s) 262, 407
Hin6I GCGC 3 cut(s) 98, 110, 334
HinP1I GCGC 3 cut(s) 98, 110, 334
HincII GTYRAC 3 cut(s) 55, 90, 120
HindII GTYRAC 3 cut(s) 55, 90, 120
HinfI GANTC 3 cut(s) 26, 149, 378
HpaII CCGG 1 cut(s) 337
HphI GGTGA 2 cut(s) 118, 202
Hpy166II GTNNAC 3 cut(s) 55, 90, 120
Hpy188I TCNGA 2 cut(s) 34, 377
Hpy188III TCNNGA 1 cut(s) 429
Hpy8I GTNNAC 3 cut(s) 55, 90, 120
Hpy99I CGWCG 4 cut(s) 94, 121, 124, 307
HpyAV CCTTC 1 cut(s) 178
HpyCH4III ACNGT 3 cut(s) 52, 87, 133
HpyCH4V TGCA 1 cut(s) 262
HpyF10VI GCNNNNNNNGC 2 cut(s) 213, 391
HpyF3I CTNAG 2 cut(s) 159, 192
Hsp92I GRCGYC 1 cut(s) 372
Hsp92II CATG 2 cut(s) 262, 407
HspAI GCGC 3 cut(s) 98, 110, 334
KroI GCCGGC 1 cut(s) 336
KroNI GCCGGC 1 cut(s) 338
Kzo9I GATC 1 cut(s) 202
LmnI GCTCC 1 cut(s) 247
Lsp1109I GCAGC 1 cut(s) 403
LweI GCATC 2 cut(s) 229, 394
MaeIII GTNAC 1 cut(s) 46
MalI GATC 1 cut(s) 204
MboI GATC 1 cut(s) 202
MboII GAAGA 4 cut(s) 52, 85, 286, 304
MluCI AATT 1 cut(s) 310
MlyI GAGTC 1 cut(s) 35
MmeI TCCRAC 1 cut(s) 213
MnlI CCTC 4 cut(s) 28, 88, 197, 218
MroNI GCCGGC 1 cut(s) 336
MspI CCGG 1 cut(s) 337
MspR9I CCNGG 1 cut(s) 179
Mva1269I GAATGC 1 cut(s) 363
MvaI CCWGG 1 cut(s) 179
MvnI CGCG 3 cut(s) 71, 216, 269
MwoI GCNNNNNNNGC 2 cut(s) 213, 391
NaeI GCCGGC 1 cut(s) 338
NdeII GATC 1 cut(s) 202
NgoMIV GCCGGC 1 cut(s) 336
NlaIII CATG 2 cut(s) 262, 407
NmuCI GTSAC 1 cut(s) 46
PcsI WCGNNNNNNNCGW 1 cut(s) 60
PctI GAATGC 1 cut(s) 363
PdiI GCCGGC 1 cut(s) 338
PfeI GAWTC 2 cut(s) 149, 378
PkrI GCNGC 2 cut(s) 271, 393
Ple19I CGATCG 1 cut(s) 205
PleI GAGTC 1 cut(s) 34
PpsI GAGTC 1 cut(s) 34
Psp6I CCWGG 1 cut(s) 177
PspGI CCWGG 1 cut(s) 177
PspPI GGNCC 1 cut(s) 181
PvuI CGATCG 1 cut(s) 205
RsaI GTAC 1 cut(s) 330
RsaNI GTAC 1 cut(s) 329
SalI GTCGAC 3 cut(s) 53, 88, 118
SatI GCNGC 2 cut(s) 270, 392
Sau3AI GATC 1 cut(s) 202
Sau96I GGNCC 1 cut(s) 181
SchI GAGTC 1 cut(s) 35
ScrFI CCNGG 1 cut(s) 179
SetI ASST 5 cut(s) 24, 160, 170, 189, 222
SfaNI GCATC 2 cut(s) 229, 394
SgrDI CGTCGACG 1 cut(s) 118
SinI GGWCC 1 cut(s) 181
SmlI CTYRAG 1 cut(s) 320
SmoI CTYRAG 1 cut(s) 320
Sse9I AATT 1 cut(s) 310
SsiI CCGC 3 cut(s) 214, 269, 282
StyD4I CCNGG 1 cut(s) 177
TaaI ACNGT 3 cut(s) 52, 87, 133
TaqI TCGA 5 cut(s) 54, 89, 119, 147, 201
TasI AATT 1 cut(s) 310
TauI GCSGC 1 cut(s) 272
TfiI GAWTC 2 cut(s) 149, 378
TscAI CASTG 1 cut(s) 55
TseFI GTSAC 1 cut(s) 46
TseI GCWGC 1 cut(s) 391
Tsp45I GTSAC 1 cut(s) 46
TspDTI ATGAA 1 cut(s) 244
TspGWI ACGGA 3 cut(s) 107, 234, 316
TspRI CASTG 1 cut(s) 55
VpaK11BI GGWCC 1 cut(s) 181
XapI RAATTY 1 cut(s) 310
XmiI GTMKAC 3 cut(s) 54, 89, 119
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.