MD05G1247000.v1.1

Protein SUPPRESSOR OF FRI

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0006315
Physical Location & Seq
Reverse (-)
37823791 .. 37828493
4703 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1247000.v1.1.491

Sequence Viewer

Length: 1068 bp
ATGGGAAAGAAGAAGAAGAGAGTGGCGTCGAAGGTGTGGTGCTACTACTGCGATAGAGAATTCGACGATGAGAAGATACTGGTGCAGCATCAGAAAGCCAAGCACTTCAAGTGCCATGTCTGCCATAAGAAGCTCTCCACCGCCGGCGGCATGGCCATTCACGTCCTCCAGGTCCACAAAGAGAGCGTCACCAAGGTTCCCAATGCGAAGGATGGCAGGGAGTCAACGGATATTGAAATTTATGGGATGCAAGGAATCCCACCTGACGTCTTGGCTGCACATTATGGAGAGGAAGAGGAAGACGGTCCATCAAAAGTAGCTAAGGTAAACATCCCAACAACCCAGTTTGTTGGTGGTATGGTGCCAGGTTCGATGGGGGTTGCATATCCTCCCCAACCACCTTTGGGTGCAATGCGGCCAATGTACAATTCTGCAGTTCCGGTGACTCCGAATGCTTGGCAAGTTCCACCTCGTCCCCAGCCATGGTATCCACAGCCTCCAGCAGTCTCAGTACCTGCTTCCTCATTGGGTTATGTGCAGCAGCCATTGTTTCCTGTGCACAATGGGAGACCACCTCTACCATCAACAACCACCCCTGGACTTCTGCCTCCACATATAGCCCCTCCTGGCCTTCCTACATCCATGCCTCCTGTTCCTGTATCACAACCCCTATTTCCTGTTGTTGGTATAAATAATGTACCAACACAAAGTTCTCCCTTTTCTGCTCCCATGCTTTCAACAAGTATTCCTTTAAATTCTCCAGCTGAAGTTAAAGGACCAACGGATGCTTATCAAGGTGTTAATTCACACTCTTATGCATCTGGTCCAAACACTGGTGGTCCATCAATTGGACCACCCCCTGTTATTGCAAACAAAGCTCCTGCTCCCCAGCCAGCTGCTAATGAGGTGTATCTAGTTTGGGATGATGAAGCTATGTCCATGGAGGAAAGAAGAATGTCCTTAGTGAAGTATCAGGTTCATGATGAAACTAGCCAGATGAGTTCAATCGATGCAGCCATAGACAGAAGGATTTTGGAGAGCAGGCTTGCTGGTCGAATGGCATTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000070 GO:0000075 GO:0000226 GO:0000278 GO:0000280 GO:0000775 GO:0000776 GO:0000819 GO:0001578 GO:0003674 GO:0003700 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005694 GO:0005730 GO:0005856 GO:0006355 GO:0006996 GO:0007010 GO:0007017 GO:0007049 GO:0007051 GO:0007052 GO:0007059 GO:0007088 GO:0007093 GO:0007094 GO:0007346 GO:0008017 GO:0008092 GO:0008150 GO:0008270 GO:0008608 GO:0009889 GO:0009987 GO:0010468 GO:0010556 GO:0010564 GO:0010639 GO:0010948 GO:0010965 GO:0015631 GO:0016043 GO:0019219 GO:0019222 GO:0022402 GO:0022607 GO:0030071 GO:0031109 GO:0031323 GO:0031326 GO:0031577 GO:0031647 GO:0031974 GO:0031981 GO:0032991 GO:0033043 GO:0033044 GO:0033045 GO:0033046 GO:0033047 GO:0033048 GO:0034622 GO:0043167 GO:0043169 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043933 GO:0044085 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044430 GO:0044446 GO:0044464 GO:0045786 GO:0045839 GO:0045841 GO:0045930 GO:0046785 GO:0046872 GO:0046914 GO:0048285 GO:0048519 GO:0048523 GO:0050789 GO:0050794 GO:0050821 GO:0051128 GO:0051129 GO:0051171 GO:0051225 GO:0051252 GO:0051258 GO:0051276 GO:0051726 GO:0051783 GO:0051784 GO:0051983 GO:0051985 GO:0060255 GO:0065003 GO:0065007 GO:0065008 GO:0070013 GO:0070925 GO:0071173 GO:0071174 GO:0071840 GO:0080090 GO:0090307 GO:0097435 GO:0098687 GO:0098813 GO:0140014 GO:0140110 GO:1901987 GO:1901988 GO:1901990 GO:1901991 GO:1902099 GO:1902100 GO:1902850 GO:1903047 GO:1903506 GO:1905818 GO:1905819 GO:1990047 GO:2000112 GO:2000816 GO:2001141 GO:2001251
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

356

Amino Acids

38.3

Weight (kDa)

8.24

Isoelectric Point (pI)

69.57

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 270
Acc36I ACCTGC 1 cut(s) 523
AccB1I GGYRCC 1 cut(s) 361
AccB7I CCANNNNNTGG 2 cut(s) 833, 848
AciI CCGC 3 cut(s) 141, 147, 415
AcoI YGGCCR 2 cut(s) 153, 416
AcsI RAATTY 3 cut(s) 59, 237, 754
AcuI CTGAAG 1 cut(s) 786
AcyI GRCGYC 2 cut(s) 26, 267
AfaI GTAC 3 cut(s) 425, 513, 699
AfiI CCNNNNNNNGG 5 cut(s) 404, 483, 683, 833, 848
AgsI TTSAA 4 cut(s) 109, 236, 738, 1005
AjiI CACGTC 1 cut(s) 163
AjnI CCWGG 4 cut(s) 168, 364, 595, 625
AjuI GAANNNNNNNTTGG 4 cut(s) 412, 444, 694, 726
AluBI AGCT 6 cut(s) 133, 320, 764, 878, 896, 932
AluI AGCT 6 cut(s) 133, 320, 764, 878, 896, 932
Alw21I GWGCWC 1 cut(s) 561
Alw26I GTCTC 2 cut(s) 511, 562
Alw44I GTGCAC 1 cut(s) 557
AlwNI CAGNNNCTG 1 cut(s) 515
AoxI GGCC 3 cut(s) 153, 416, 628
ApaLI GTGCAC 1 cut(s) 557
ApeKI GCWGC 6 cut(s) 85, 275, 538, 541, 896, 1013
ApoI RAATTY 3 cut(s) 59, 237, 754
ArsI GACNNNNNNTTYG 2 cut(s) 171, 203
AspS9I GGNCC 6 cut(s) 172, 305, 776, 824, 839, 851
AsuHPI GGTGA 2 cut(s) 181, 454
AvaII GGWCC 6 cut(s) 172, 305, 776, 824, 839, 851
BaeGI GKGCMC 1 cut(s) 561
BalI TGGCCA 1 cut(s) 155
BanI GGYRCC 1 cut(s) 361
BbsI GAAGAC 1 cut(s) 306
Bbv12I GWGCWC 1 cut(s) 561
BbvI GCAGC 6 cut(s) 97, 262, 550, 553, 883, 1025
BccI CCATC 5 cut(s) 206, 316, 367, 589, 850
BciT130I CCWGG 4 cut(s) 170, 366, 597, 627
BciVI GTATCC 1 cut(s) 498
BcoDI GTCTC 2 cut(s) 511, 562
BfaI CTAG 2 cut(s) 914, 990
BfmI CTRYAG 1 cut(s) 432
BfuAI ACCTGC 1 cut(s) 523
BfuI GTATCC 1 cut(s) 498
BisI GCNGC 8 cut(s) 86, 148, 276, 416, 539, 542, 897, 1014
BlsI GCNGC 8 cut(s) 87, 149, 277, 417, 540, 543, 898, 1015
Bme1390I CCNGG 4 cut(s) 170, 366, 597, 627
Bme18I GGWCC 6 cut(s) 172, 305, 776, 824, 839, 851
BmgBI CACGTC 1 cut(s) 163
BmgT120I GGNCC 6 cut(s) 172, 305, 776, 824, 839, 851
BmiI GGNNCC 2 cut(s) 198, 363
BmrFI CCNGG 4 cut(s) 170, 366, 597, 627
BmrI ACTGGG 1 cut(s) 337
BmsI GCATC 5 cut(s) 97, 237, 775, 827, 1000
BmuI ACTGGG 1 cut(s) 337
BpiI GAAGAC 1 cut(s) 306
BplI GAGNNNNNCTC 2 cut(s) 559, 591
BpmI CTGGAG 3 cut(s) 152, 483, 744
Bpu10I CCTNAGC 1 cut(s) 321
Bsa29I ATCGAT 1 cut(s) 1008
BsaBI GATNNNNATC 1 cut(s) 789
BsaHI GRCGYC 2 cut(s) 26, 267
BsaI GGTCTC 1 cut(s) 562
BsaJI CCNNGG 4 cut(s) 192, 482, 595, 939
BsaWI WCCGGW 1 cut(s) 439
Bsc4I CCNNNNNNNGG 5 cut(s) 404, 483, 683, 833, 848
Bse118I RCCGGY 1 cut(s) 143
Bse1I ACTGG 3 cut(s) 84, 343, 838
Bse3DI GCAATG 1 cut(s) 417
Bse8I GATNNNNATC 1 cut(s) 789
BseBI CCWGG 4 cut(s) 170, 366, 597, 627
BseCI ATCGAT 1 cut(s) 1008
BseDI CCNNGG 4 cut(s) 192, 482, 595, 939
BseGI GGATG 6 cut(s) 217, 252, 330, 638, 790, 928
BseJI GATNNNNATC 1 cut(s) 789
BseLI CCNNNNNNNGG 5 cut(s) 404, 483, 683, 833, 848
BseMI GCAATG 1 cut(s) 417
BseMII CTCAG 1 cut(s) 522
BseNI ACTGG 3 cut(s) 84, 343, 838
BseSI GKGCMC 1 cut(s) 561
BseXI GCAGC 6 cut(s) 97, 262, 550, 553, 883, 1025
BseYI CCCAGC 2 cut(s) 477, 888
BsgI GTGCAG 3 cut(s) 104, 261, 557
BshFI GGCC 3 cut(s) 155, 418, 630
BshNI GGYRCC 1 cut(s) 361
BshVI ATCGAT 1 cut(s) 1008
BsiHKAI GWGCWC 1 cut(s) 561
BsiSI CCGG 2 cut(s) 144, 440
BslFI GGGAC 1 cut(s) 459
BslI CCNNNNNNNGG 5 cut(s) 404, 483, 683, 833, 848
BsmAI GTCTC 2 cut(s) 511, 562
BsmFI GGGAC 1 cut(s) 459
BsmI GAATGC 1 cut(s) 457
BsnI GGCC 3 cut(s) 155, 418, 630
Bso31I GGTCTC 1 cut(s) 562
Bsp1286I GDGCHC 1 cut(s) 561
Bsp1407I TGTACA 1 cut(s) 423
Bsp19I CCATGG 2 cut(s) 482, 939
BspACI CCGC 3 cut(s) 141, 147, 415
BspANI GGCC 3 cut(s) 155, 418, 630
BspCNI CTCAG 1 cut(s) 521
BspDI ATCGAT 1 cut(s) 1008
BspHI TCATGA 1 cut(s) 979
BspLI GGNNCC 2 cut(s) 198, 363
BspMAI CTGCAG 1 cut(s) 436
BspMI ACCTGC 1 cut(s) 523
BspT107I GGYRCC 1 cut(s) 361
BspTNI GGTCTC 1 cut(s) 562
BsrDI GCAATG 1 cut(s) 417
BsrFI RCCGGY 1 cut(s) 143
BsrGI TGTACA 1 cut(s) 423
BsrI ACTGG 3 cut(s) 84, 343, 838
BssAI RCCGGY 1 cut(s) 143
BssECI CCNNGG 4 cut(s) 192, 482, 595, 939
BssNI GRCGYC 2 cut(s) 26, 267
BssT1I CCWWGG 3 cut(s) 192, 482, 939
Bst2UI CCWGG 4 cut(s) 170, 366, 597, 627
Bst4CI ACNGT 1 cut(s) 305
Bst6I CTCTTC 2 cut(s) 11, 288
BstACI GRCGYC 2 cut(s) 26, 267
BstAUI TGTACA 1 cut(s) 423
BstC8I GCNNGC 4 cut(s) 145, 894, 1043, 1047
BstDEI CTNAG 3 cut(s) 321, 508, 961
BstDSI CCRYGG 2 cut(s) 482, 939
BstF5I GGATG 6 cut(s) 217, 252, 330, 638, 790, 928
BstMAI GTCTC 2 cut(s) 511, 562
BstMWI GCNNNNNNNGC 3 cut(s) 48, 120, 875
BstNI CCWGG 4 cut(s) 170, 366, 597, 627
BstSCI CCNGG 4 cut(s) 168, 364, 595, 625
BstSFI CTRYAG 1 cut(s) 432
BstSLI GKGCMC 1 cut(s) 561
BstV1I GCAGC 6 cut(s) 97, 262, 550, 553, 883, 1025
BstV2I GAAGAC 1 cut(s) 306
BstXI CCANNNNNNTGG 1 cut(s) 350
Bsu15I ATCGAT 1 cut(s) 1008
BsuI GTATCC 1 cut(s) 498
BsuRI GGCC 3 cut(s) 155, 418, 630
BsuTUI ATCGAT 1 cut(s) 1008
BtgI CCRYGG 2 cut(s) 482, 939
BtrI CACGTC 1 cut(s) 163
BtsCI GGATG 6 cut(s) 217, 252, 330, 638, 790, 928
BtsIMutI CAGTG 1 cut(s) 831
BveI ACCTGC 1 cut(s) 523
Cac8I GCNNGC 4 cut(s) 145, 894, 1043, 1047
CaiI CAGNNNCTG 1 cut(s) 515
CciI TCATGA 1 cut(s) 979
Cfr10I RCCGGY 1 cut(s) 143
Cfr13I GGNCC 6 cut(s) 172, 305, 776, 824, 839, 851
ClaI ATCGAT 1 cut(s) 1008
CseI GACGC 2 cut(s) 15, 175
Csp6I GTAC 3 cut(s) 424, 512, 698
CviAII CATG 7 cut(s) 116, 151, 483, 643, 730, 940, 980
CviQI GTAC 3 cut(s) 424, 512, 698
DdeI CTNAG 3 cut(s) 321, 508, 961
DraI TTTAAA 1 cut(s) 753
EaeI YGGCCR 2 cut(s) 153, 416
Eam1104I CTCTTC 2 cut(s) 11, 288
EarI CTCTTC 2 cut(s) 11, 288
Eco130I CCWWGG 3 cut(s) 192, 482, 939
Eco31I GGTCTC 1 cut(s) 562
Eco47I GGWCC 6 cut(s) 172, 305, 776, 824, 839, 851
Eco57I CTGAAG 1 cut(s) 786
EcoRI GAATTC 1 cut(s) 59
EcoRII CCWGG 4 cut(s) 168, 364, 595, 625
EcoT14I CCWWGG 3 cut(s) 192, 482, 939
EcoT22I ATGCAT 1 cut(s) 820
ErhI CCWWGG 3 cut(s) 192, 482, 939
FaeI CATG 7 cut(s) 119, 154, 486, 646, 733, 943, 983
FalI AAGNNNNNCTT 2 cut(s) 733, 765
FaqI GGGAC 1 cut(s) 459
FatI CATG 7 cut(s) 115, 150, 482, 642, 729, 939, 979
Fnu4HI GCNGC 8 cut(s) 86, 148, 276, 416, 539, 542, 897, 1014
FokI GGATG 6 cut(s) 224, 259, 317, 625, 797, 935
Fsp4HI GCNGC 8 cut(s) 86, 148, 276, 416, 539, 542, 897, 1014
FspBI CTAG 2 cut(s) 914, 990
GluI GCNGC 8 cut(s) 86, 148, 276, 416, 539, 542, 897, 1014
GsaI CCCAGC 2 cut(s) 481, 892
GsuI CTGGAG 3 cut(s) 152, 483, 744
HaeIII GGCC 3 cut(s) 155, 418, 630
HapII CCGG 2 cut(s) 144, 440
HgaI GACGC 2 cut(s) 15, 175
Hin1I GRCGYC 2 cut(s) 26, 267
Hin1II CATG 7 cut(s) 119, 154, 486, 646, 733, 943, 983
HincII GTYRAC 1 cut(s) 225
HindII GTYRAC 1 cut(s) 225
HinfI GANTC 3 cut(s) 221, 255, 445
HpaII CCGG 2 cut(s) 144, 440
HphI GGTGA 2 cut(s) 181, 454
Hpy166II GTNNAC 4 cut(s) 175, 225, 328, 559
Hpy188I TCNGA 2 cut(s) 93, 450
Hpy188III TCNNGA 1 cut(s) 980
Hpy8I GTNNAC 4 cut(s) 175, 225, 328, 559
Hpy99I CGWCG 2 cut(s) 31, 68
HpyAV CCTTC 4 cut(s) 25, 202, 641, 1020
HpyCH4III ACNGT 1 cut(s) 305
HpyCH4IV ACGT 2 cut(s) 162, 267
HpyF10VI GCNNNNNNNGC 3 cut(s) 48, 120, 875
HpyF3I CTNAG 3 cut(s) 321, 508, 961
HpySE526I ACGT 2 cut(s) 162, 267
Hsp92I GRCGYC 2 cut(s) 26, 267
Hsp92II CATG 7 cut(s) 119, 154, 486, 646, 733, 943, 983
KroI GCCGGC 1 cut(s) 143
KroNI GCCGGC 1 cut(s) 145
LmnI GCTCC 3 cut(s) 730, 883, 889
Lsp1109I GCAGC 6 cut(s) 97, 262, 550, 553, 883, 1025
LweI GCATC 5 cut(s) 97, 237, 775, 827, 1000
MaeI CTAG 2 cut(s) 914, 990
MaeII ACGT 2 cut(s) 162, 267
MaeIII GTNAC 2 cut(s) 187, 442
MboII GAAGA 7 cut(s) 22, 25, 28, 85, 305, 311, 963
MfeI CAATTG 1 cut(s) 846
MhlI GDGCHC 1 cut(s) 561
MlsI TGGCCA 1 cut(s) 155
MluCI AATT 6 cut(s) 59, 237, 427, 754, 802, 846
MluNI TGGCCA 1 cut(s) 155
MlyI GAGTC 2 cut(s) 230, 439
Mox20I TGGCCA 1 cut(s) 155
Mph1103I ATGCAT 1 cut(s) 820
MreI CGCCGGCG 1 cut(s) 143
MroNI GCCGGC 1 cut(s) 143
MscI TGGCCA 1 cut(s) 155
MseI TTAA 3 cut(s) 752, 771, 801
MslI CAYNNNNRTG 1 cut(s) 813
Msp20I TGGCCA 1 cut(s) 155
MspA1I CMGCKG 2 cut(s) 764, 896
MspI CCGG 2 cut(s) 144, 440
MspR9I CCNGG 4 cut(s) 170, 366, 597, 627
MunI CAATTG 1 cut(s) 846
Mva1269I GAATGC 1 cut(s) 457
MvaI CCWGG 4 cut(s) 170, 366, 597, 627
MwoI GCNNNNNNNGC 3 cut(s) 48, 120, 875
NaeI GCCGGC 1 cut(s) 145
NcoI CCATGG 2 cut(s) 482, 939
NgoMIV GCCGGC 1 cut(s) 143
NlaIII CATG 7 cut(s) 119, 154, 486, 646, 733, 943, 983
NlaIV GGNNCC 2 cut(s) 198, 363
NmuCI GTSAC 2 cut(s) 187, 442
NsiI ATGCAT 1 cut(s) 820
PagI TCATGA 1 cut(s) 979
PctI GAATGC 1 cut(s) 457
PdiI GCCGGC 1 cut(s) 145
PfeI GAWTC 1 cut(s) 255
PflMI CCANNNNNTGG 2 cut(s) 833, 848
PkrI GCNGC 8 cut(s) 87, 149, 277, 417, 540, 543, 898, 1015
PleI GAGTC 2 cut(s) 229, 439
PpsI GAGTC 2 cut(s) 229, 439
Psp6I CCWGG 4 cut(s) 168, 364, 595, 625
PspFI CCCAGC 2 cut(s) 477, 888
PspGI CCWGG 4 cut(s) 168, 364, 595, 625
PspN4I GGNNCC 2 cut(s) 198, 363
PspPI GGNCC 6 cut(s) 172, 305, 776, 824, 839, 851
PstI CTGCAG 1 cut(s) 436
PstNI CAGNNNCTG 1 cut(s) 515
PvuII CAGCTG 2 cut(s) 764, 896
RsaI GTAC 3 cut(s) 425, 513, 699
RsaNI GTAC 3 cut(s) 424, 512, 698
RseI CAYNNNNRTG 1 cut(s) 813
SaqAI TTAA 3 cut(s) 752, 771, 801
SatI GCNGC 8 cut(s) 86, 148, 276, 416, 539, 542, 897, 1014
Sau96I GGNCC 6 cut(s) 172, 305, 776, 824, 839, 851
SchI GAGTC 2 cut(s) 230, 439
ScrFI CCNGG 4 cut(s) 170, 366, 597, 627
SduI GDGCHC 1 cut(s) 561
SfaNI GCATC 5 cut(s) 97, 237, 775, 827, 1000
SfcI CTRYAG 1 cut(s) 432
SgrAI CRCCGGYG 1 cut(s) 143
SinI GGWCC 6 cut(s) 172, 305, 776, 824, 839, 851
SmiMI CAYNNNNRTG 1 cut(s) 813
Sse9I AATT 6 cut(s) 59, 237, 427, 754, 802, 846
SsiI CCGC 3 cut(s) 141, 147, 415
SspMI CTAG 2 cut(s) 914, 990
StyD4I CCNGG 4 cut(s) 168, 364, 595, 625
StyI CCWWGG 3 cut(s) 192, 482, 939
TaaI ACNGT 1 cut(s) 305
TaiI ACGT 2 cut(s) 165, 270
TaqI TCGA 5 cut(s) 29, 63, 371, 1008, 1054
TasI AATT 6 cut(s) 59, 237, 427, 754, 802, 846
TatI WGTACW 1 cut(s) 423
TauI GCSGC 2 cut(s) 150, 418
TfiI GAWTC 1 cut(s) 255
Tru1I TTAA 3 cut(s) 752, 771, 801
Tru9I TTAA 3 cut(s) 752, 771, 801
TscAI CASTG 1 cut(s) 838
TseFI GTSAC 2 cut(s) 187, 442
TseI GCWGC 6 cut(s) 85, 275, 538, 541, 896, 1013
Tsp45I GTSAC 2 cut(s) 187, 442
TspDTI ATGAA 3 cut(s) 942, 968, 999
TspGWI ACGGA 2 cut(s) 242, 797
TspRI CASTG 1 cut(s) 838
Van91I CCANNNNNTGG 2 cut(s) 833, 848
VneI GTGCAC 1 cut(s) 557
VpaK11BI GGWCC 6 cut(s) 172, 305, 776, 824, 839, 851
XapI RAATTY 3 cut(s) 59, 237, 754
XcmI CCANNNNNNNNNTGG 1 cut(s) 350
XspI CTAG 2 cut(s) 914, 990
ZraI GACGTC 1 cut(s) 268
Zsp2I ATGCAT 1 cut(s) 820
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.