MD10G1227600.v1.1

Protein SUPPRESSOR OF FRI

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0006315
Physical Location & Seq
Reverse (-)
32444573 .. 32449441
4869 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1227600.v1.1.491

Sequence Viewer

Length: 1071 bp
ATGGGAAAGAAGAAGAAGAGAGTGGCGTCGAAGGTGTGGTGTTACTACTGCGATAGAGAGTTCGACGATGAGAAGATACTGGTGCAGCACCAGAAAGCCAAGCATTTTAAGTGCCATGTCTGCCACAAGAAGCTCTCCACCGCCGGCGGCATGGCCATTCACGTCCTCCAGGTCCACAAAGAGAGCGTCACCAAGGTTCCCAATGCGAAGGCTGACAGGGAGTCGACGGATATTGAAATATATGGGATGCAAGGAATCCCACCTCACGTTTTGGCTGCACATTATGGAGAGGAAGATGAAGATGGTCCATCAAAAGTAGCTAAAGTAGACATCCCAACAACCCAGTTTGTTGGTGGTATGGTGCCAGGTTCGATGGGGATTGCATATCCTCCCCAACCACCTTTGGGTGCAATGCGGCCAATGTACAGTTCTGCAGTTCGAGTGCCTCCGAATGGTTGGCAAGTTCCACCTCGTCCCCAGCCATGGTATCCACAGCCTCCATCAGTCTCAGTACCTCCTCCTTCCTCATTGGGTTATGTGCAGCAACCATTGTTCCCTGTGCAGAATGGGAGGCCACCTCTACCATCAACAACAATCCCCGGACCTCTGCCTCCACATATAGCTCCTCCTGGGCTTACTACATCCATGCCTCCTGCTCCTGTATCACAACCCCTGTTTCCTGTTGTTGGTATAAATAATGTACCAACACAAAGTTCTCCCTTTTCTCCTCCTTTGCTTTCAATCAGTGTTCCCTTTAGTTCTCCAGCTGAAGTTAAAGGCTCAACAGATGCTTATCAAGGTGTTAATTCACACTCTTATGCATCTGGTCCGAACACTGGTGGTCCATCAATTGGACCACCCCCTGTAATTGCAAACAAAGCTCCTGCTCCCCAGCCAGCTGCTAATGAGGTGTATCTAGTTTGGGATGATGAGGCTATGTCCATGGAGGAAAGAAGAATGTCATTGGCAAAGTATCAGGTGCATGATGAAACTAGCCAGATGAGTTCAATTGATGCTGCCATAGACAGAAGGATTATGGAGGGCAGGCTTGCTGGTCGAATGGCATTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000070 GO:0000075 GO:0000226 GO:0000278 GO:0000280 GO:0000775 GO:0000776 GO:0000819 GO:0001578 GO:0003674 GO:0003700 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005694 GO:0005730 GO:0005856 GO:0006355 GO:0006996 GO:0007010 GO:0007017 GO:0007049 GO:0007051 GO:0007052 GO:0007059 GO:0007088 GO:0007093 GO:0007094 GO:0007346 GO:0008017 GO:0008092 GO:0008150 GO:0008270 GO:0008608 GO:0009889 GO:0009987 GO:0010468 GO:0010556 GO:0010564 GO:0010639 GO:0010948 GO:0010965 GO:0015631 GO:0016043 GO:0019219 GO:0019222 GO:0022402 GO:0022607 GO:0030071 GO:0031109 GO:0031323 GO:0031326 GO:0031577 GO:0031647 GO:0031974 GO:0031981 GO:0032991 GO:0033043 GO:0033044 GO:0033045 GO:0033046 GO:0033047 GO:0033048 GO:0034622 GO:0043167 GO:0043169 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043933 GO:0044085 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044430 GO:0044446 GO:0044464 GO:0045786 GO:0045839 GO:0045841 GO:0045930 GO:0046785 GO:0046872 GO:0046914 GO:0048285 GO:0048519 GO:0048523 GO:0050789 GO:0050794 GO:0050821 GO:0051128 GO:0051129 GO:0051171 GO:0051225 GO:0051252 GO:0051258 GO:0051276 GO:0051726 GO:0051783 GO:0051784 GO:0051983 GO:0051985 GO:0060255 GO:0065003 GO:0065007 GO:0065008 GO:0070013 GO:0070925 GO:0071173 GO:0071174 GO:0071840 GO:0080090 GO:0090307 GO:0097435 GO:0098687 GO:0098813 GO:0140014 GO:0140110 GO:1901987 GO:1901988 GO:1901990 GO:1901991 GO:1902099 GO:1902100 GO:1902850 GO:1903047 GO:1903506 GO:1905818 GO:1905819 GO:1990047 GO:2000112 GO:2000816 GO:2001141 GO:2001251
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

357

Amino Acids

38.42

Weight (kDa)

8.54

Isoelectric Point (pI)

68.09

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 361
AccB7I CCANNNNNTGG 1 cut(s) 851
AccI GTMKAC 2 cut(s) 224, 327
AciI CCGC 3 cut(s) 141, 147, 415
AcoI YGGCCR 2 cut(s) 153, 416
AcuI CTGAAG 1 cut(s) 789
AcyI GRCGYC 1 cut(s) 26
AfaI GTAC 3 cut(s) 425, 513, 702
AfiI CCNNNNNNNGG 6 cut(s) 404, 452, 483, 686, 836, 851
AgsI TTSAA 3 cut(s) 236, 741, 1008
AhdI GACNNNNNGTC 1 cut(s) 220
AjiI CACGTC 1 cut(s) 163
AjnI CCWGG 3 cut(s) 168, 364, 628
AjuI GAANNNNNNNTTGG 4 cut(s) 412, 444, 697, 729
AluBI AGCT 6 cut(s) 133, 320, 623, 767, 881, 899
AluI AGCT 6 cut(s) 133, 320, 623, 767, 881, 899
Alw26I GTCTC 1 cut(s) 511
AoxI GGCC 3 cut(s) 153, 416, 572
ApeKI GCWGC 5 cut(s) 85, 275, 541, 899, 1016
ArsI GACNNNNNNTTYG 2 cut(s) 171, 203
AspS9I GGNCC 6 cut(s) 172, 305, 602, 827, 842, 854
AsuC2I CCSGG 1 cut(s) 600
AsuHPI GGTGA 1 cut(s) 181
AvaII GGWCC 6 cut(s) 172, 305, 602, 827, 842, 854
BalI TGGCCA 1 cut(s) 155
BanI GGYRCC 1 cut(s) 361
BbvI GCAGC 5 cut(s) 97, 262, 553, 886, 1003
BccI CCATC 6 cut(s) 296, 316, 367, 508, 592, 853
BciT130I CCWGG 3 cut(s) 170, 366, 630
BciVI GTATCC 1 cut(s) 498
BcnI CCSGG 1 cut(s) 600
BcoDI GTCTC 1 cut(s) 511
BfaI CTAG 2 cut(s) 917, 993
BfmI CTRYAG 1 cut(s) 432
BfuI GTATCC 1 cut(s) 498
BisI GCNGC 7 cut(s) 86, 148, 276, 416, 542, 900, 1017
BlsI GCNGC 7 cut(s) 87, 149, 277, 417, 543, 901, 1018
Bme1390I CCNGG 4 cut(s) 170, 366, 600, 630
Bme18I GGWCC 6 cut(s) 172, 305, 602, 827, 842, 854
BmeRI GACNNNNNGTC 1 cut(s) 220
BmgBI CACGTC 1 cut(s) 163
BmgT120I GGNCC 6 cut(s) 172, 305, 602, 827, 842, 854
BmiI GGNNCC 2 cut(s) 198, 363
BmrFI CCNGG 4 cut(s) 170, 366, 600, 630
BmrI ACTGGG 1 cut(s) 337
BmsI GCATC 4 cut(s) 237, 778, 830, 1003
BmuI ACTGGG 1 cut(s) 337
BplI GAGNNNNNCTC 2 cut(s) 562, 594
BpmI CTGGAG 2 cut(s) 152, 747
BpuMI CCSGG 1 cut(s) 600
BsaBI GATNNNNATC 1 cut(s) 792
BsaHI GRCGYC 1 cut(s) 26
BsaJI CCNNGG 5 cut(s) 192, 482, 598, 629, 942
Bsc4I CCNNNNNNNGG 6 cut(s) 404, 452, 483, 686, 836, 851
Bse118I RCCGGY 1 cut(s) 143
Bse1I ACTGG 3 cut(s) 84, 343, 841
Bse3DI GCAATG 1 cut(s) 417
Bse8I GATNNNNATC 1 cut(s) 792
BseBI CCWGG 3 cut(s) 170, 366, 630
BseDI CCNNGG 5 cut(s) 192, 482, 598, 629, 942
BseGI GGATG 4 cut(s) 252, 330, 641, 931
BseJI GATNNNNATC 1 cut(s) 792
BseLI CCNNNNNNNGG 6 cut(s) 404, 452, 483, 686, 836, 851
BseMI GCAATG 1 cut(s) 417
BseMII CTCAG 1 cut(s) 522
BseNI ACTGG 3 cut(s) 84, 343, 841
BseRI GAGGAG 3 cut(s) 507, 615, 717
BseXI GCAGC 5 cut(s) 97, 262, 553, 886, 1003
BseYI CCCAGC 2 cut(s) 477, 891
BsgI GTGCAG 4 cut(s) 104, 261, 560, 581
BshFI GGCC 3 cut(s) 155, 418, 574
BshNI GGYRCC 1 cut(s) 361
BsiSI CCGG 2 cut(s) 144, 600
BslFI GGGAC 1 cut(s) 459
BslI CCNNNNNNNGG 6 cut(s) 404, 452, 483, 686, 836, 851
BsmAI GTCTC 1 cut(s) 511
BsmFI GGGAC 1 cut(s) 459
BsnI GGCC 3 cut(s) 155, 418, 574
Bsp1407I TGTACA 1 cut(s) 423
Bsp19I CCATGG 2 cut(s) 482, 942
BspACI CCGC 3 cut(s) 141, 147, 415
BspANI GGCC 3 cut(s) 155, 418, 574
BspCNI CTCAG 1 cut(s) 521
BspLI GGNNCC 2 cut(s) 198, 363
BspMAI CTGCAG 1 cut(s) 436
BspT107I GGYRCC 1 cut(s) 361
BsrDI GCAATG 1 cut(s) 417
BsrFI RCCGGY 1 cut(s) 143
BsrGI TGTACA 1 cut(s) 423
BsrI ACTGG 3 cut(s) 84, 343, 841
BssAI RCCGGY 1 cut(s) 143
BssECI CCNNGG 5 cut(s) 192, 482, 598, 629, 942
BssNI GRCGYC 1 cut(s) 26
BssT1I CCWWGG 3 cut(s) 192, 482, 942
Bst2UI CCWGG 3 cut(s) 170, 366, 630
Bst4CI ACNGT 1 cut(s) 428
Bst6I CTCTTC 1 cut(s) 11
BstACI GRCGYC 1 cut(s) 26
BstAUI TGTACA 1 cut(s) 423
BstC8I GCNNGC 4 cut(s) 145, 897, 1046, 1050
BstDEI CTNAG 1 cut(s) 508
BstDSI CCRYGG 2 cut(s) 482, 942
BstF5I GGATG 4 cut(s) 252, 330, 641, 931
BstMAI GTCTC 1 cut(s) 511
BstMWI GCNNNNNNNGC 2 cut(s) 120, 878
BstNI CCWGG 3 cut(s) 170, 366, 630
BstSCI CCNGG 4 cut(s) 168, 364, 598, 628
BstSFI CTRYAG 1 cut(s) 432
BstV1I GCAGC 5 cut(s) 97, 262, 553, 886, 1003
BstXI CCANNNNNNTGG 1 cut(s) 350
BsuI GTATCC 1 cut(s) 498
BsuRI GGCC 3 cut(s) 155, 418, 574
BtgI CCRYGG 2 cut(s) 482, 942
BtrI CACGTC 1 cut(s) 163
BtsCI GGATG 4 cut(s) 252, 330, 641, 931
BtsIMutI CAGTG 2 cut(s) 751, 834
Cac8I GCNNGC 4 cut(s) 145, 897, 1046, 1050
Cfr10I RCCGGY 1 cut(s) 143
Cfr13I GGNCC 6 cut(s) 172, 305, 602, 827, 842, 854
CseI GACGC 2 cut(s) 15, 175
Csp6I GTAC 3 cut(s) 424, 512, 701
CviAII CATG 6 cut(s) 116, 151, 483, 646, 943, 983
CviQI GTAC 3 cut(s) 424, 512, 701
DdeI CTNAG 1 cut(s) 508
DriI GACNNNNNGTC 1 cut(s) 220
EaeI YGGCCR 2 cut(s) 153, 416
Eam1104I CTCTTC 1 cut(s) 11
Eam1105I GACNNNNNGTC 1 cut(s) 220
EarI CTCTTC 1 cut(s) 11
Eco130I CCWWGG 3 cut(s) 192, 482, 942
Eco47I GGWCC 6 cut(s) 172, 305, 602, 827, 842, 854
Eco57I CTGAAG 1 cut(s) 789
EcoRII CCWGG 3 cut(s) 168, 364, 628
EcoT14I CCWWGG 3 cut(s) 192, 482, 942
EcoT22I ATGCAT 1 cut(s) 823
ErhI CCWWGG 3 cut(s) 192, 482, 942
FaeI CATG 6 cut(s) 119, 154, 486, 649, 946, 986
FaqI GGGAC 1 cut(s) 459
FatI CATG 6 cut(s) 115, 150, 482, 645, 942, 982
FblI GTMKAC 2 cut(s) 224, 327
Fnu4HI GCNGC 7 cut(s) 86, 148, 276, 416, 542, 900, 1017
FokI GGATG 4 cut(s) 259, 317, 628, 938
Fsp4HI GCNGC 7 cut(s) 86, 148, 276, 416, 542, 900, 1017
FspBI CTAG 2 cut(s) 917, 993
GluI GCNGC 7 cut(s) 86, 148, 276, 416, 542, 900, 1017
GsaI CCCAGC 2 cut(s) 481, 895
GsuI CTGGAG 2 cut(s) 152, 747
HaeIII GGCC 3 cut(s) 155, 418, 574
HapII CCGG 2 cut(s) 144, 600
HgaI GACGC 2 cut(s) 15, 175
Hin1I GRCGYC 1 cut(s) 26
Hin1II CATG 6 cut(s) 119, 154, 486, 649, 946, 986
HincII GTYRAC 1 cut(s) 225
HindII GTYRAC 1 cut(s) 225
HinfI GANTC 2 cut(s) 221, 255
HpaII CCGG 2 cut(s) 144, 600
HphI GGTGA 1 cut(s) 181
Hpy166II GTNNAC 3 cut(s) 175, 225, 328
Hpy188I TCNGA 2 cut(s) 450, 831
Hpy8I GTNNAC 3 cut(s) 175, 225, 328
Hpy99I CGWCG 3 cut(s) 31, 68, 229
HpyAV CCTTC 4 cut(s) 25, 202, 531, 1023
HpyCH4III ACNGT 1 cut(s) 428
HpyCH4IV ACGT 2 cut(s) 162, 267
HpyF10VI GCNNNNNNNGC 2 cut(s) 120, 878
HpyF3I CTNAG 1 cut(s) 508
HpySE526I ACGT 2 cut(s) 162, 267
Hsp92I GRCGYC 1 cut(s) 26
Hsp92II CATG 6 cut(s) 119, 154, 486, 649, 946, 986
KroI GCCGGC 1 cut(s) 143
KroNI GCCGGC 1 cut(s) 145
LmnI GCTCC 4 cut(s) 628, 661, 886, 892
Lsp1109I GCAGC 5 cut(s) 97, 262, 553, 886, 1003
LweI GCATC 4 cut(s) 237, 778, 830, 1003
MaeI CTAG 2 cut(s) 917, 993
MaeII ACGT 2 cut(s) 162, 267
MaeIII GTNAC 2 cut(s) 41, 187
MboII GAAGA 7 cut(s) 22, 25, 28, 85, 305, 311, 966
MfeI CAATTG 2 cut(s) 849, 1008
MlsI TGGCCA 1 cut(s) 155
MluCI AATT 4 cut(s) 805, 849, 867, 1008
MluNI TGGCCA 1 cut(s) 155
MlyI GAGTC 1 cut(s) 230
Mox20I TGGCCA 1 cut(s) 155
Mph1103I ATGCAT 1 cut(s) 823
MreI CGCCGGCG 1 cut(s) 143
MroNI GCCGGC 1 cut(s) 143
MscI TGGCCA 1 cut(s) 155
MseI TTAA 3 cut(s) 108, 774, 804
MslI CAYNNNNRTG 1 cut(s) 816
Msp20I TGGCCA 1 cut(s) 155
MspA1I CMGCKG 2 cut(s) 767, 899
MspI CCGG 2 cut(s) 144, 600
MspR9I CCNGG 4 cut(s) 170, 366, 600, 630
MunI CAATTG 2 cut(s) 849, 1008
MvaI CCWGG 3 cut(s) 170, 366, 630
MwoI GCNNNNNNNGC 2 cut(s) 120, 878
NaeI GCCGGC 1 cut(s) 145
NciI CCSGG 1 cut(s) 600
NcoI CCATGG 2 cut(s) 482, 942
NgoMIV GCCGGC 1 cut(s) 143
NlaIII CATG 6 cut(s) 119, 154, 486, 649, 946, 986
NlaIV GGNNCC 2 cut(s) 198, 363
NmuCI GTSAC 1 cut(s) 187
NsiI ATGCAT 1 cut(s) 823
PdiI GCCGGC 1 cut(s) 145
PfeI GAWTC 1 cut(s) 255
PflMI CCANNNNNTGG 1 cut(s) 851
PkrI GCNGC 7 cut(s) 87, 149, 277, 417, 543, 901, 1018
PleI GAGTC 1 cut(s) 229
PpsI GAGTC 1 cut(s) 229
Psp6I CCWGG 3 cut(s) 168, 364, 628
PspFI CCCAGC 2 cut(s) 477, 891
PspGI CCWGG 3 cut(s) 168, 364, 628
PspN4I GGNNCC 2 cut(s) 198, 363
PspPI GGNCC 6 cut(s) 172, 305, 602, 827, 842, 854
PstI CTGCAG 1 cut(s) 436
PvuII CAGCTG 2 cut(s) 767, 899
RsaI GTAC 3 cut(s) 425, 513, 702
RsaNI GTAC 3 cut(s) 424, 512, 701
RseI CAYNNNNRTG 1 cut(s) 816
SalI GTCGAC 1 cut(s) 223
SaqAI TTAA 3 cut(s) 108, 774, 804
SatI GCNGC 7 cut(s) 86, 148, 276, 416, 542, 900, 1017
Sau96I GGNCC 6 cut(s) 172, 305, 602, 827, 842, 854
SchI GAGTC 1 cut(s) 230
ScrFI CCNGG 4 cut(s) 170, 366, 600, 630
SfaNI GCATC 4 cut(s) 237, 778, 830, 1003
SfcI CTRYAG 1 cut(s) 432
SgrAI CRCCGGYG 1 cut(s) 143
SinI GGWCC 6 cut(s) 172, 305, 602, 827, 842, 854
SmiMI CAYNNNNRTG 1 cut(s) 816
Sse9I AATT 4 cut(s) 805, 849, 867, 1008
SsiI CCGC 3 cut(s) 141, 147, 415
SspMI CTAG 2 cut(s) 917, 993
StyD4I CCNGG 4 cut(s) 168, 364, 598, 628
StyI CCWWGG 3 cut(s) 192, 482, 942
TaaI ACNGT 1 cut(s) 428
TaiI ACGT 2 cut(s) 165, 270
TaqI TCGA 6 cut(s) 29, 63, 224, 371, 439, 1057
TasI AATT 4 cut(s) 805, 849, 867, 1008
TatI WGTACW 1 cut(s) 423
TauI GCSGC 2 cut(s) 150, 418
TfiI GAWTC 1 cut(s) 255
Tru1I TTAA 3 cut(s) 108, 774, 804
Tru9I TTAA 3 cut(s) 108, 774, 804
TscAI CASTG 2 cut(s) 751, 841
TseFI GTSAC 1 cut(s) 187
TseI GCWGC 5 cut(s) 85, 275, 541, 899, 1016
Tsp45I GTSAC 1 cut(s) 187
TspDTI ATGAA 2 cut(s) 312, 1002
TspGWI ACGGA 1 cut(s) 242
TspRI CASTG 2 cut(s) 751, 841
Van91I CCANNNNNTGG 1 cut(s) 851
VpaK11BI GGWCC 6 cut(s) 172, 305, 602, 827, 842, 854
XcmI CCANNNNNNNNNTGG 1 cut(s) 350
XmiI GTMKAC 2 cut(s) 224, 327
XspI CTAG 2 cut(s) 917, 993
Zsp2I ATGCAT 1 cut(s) 823
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.