Rmu_ssc0000244.1_g000016

Protein SUPPRESSOR OF FRI

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_ssc0000244.1
OG0006315
Physical Location & Seq
Forward (+)
93908 .. 97659
3752 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_ssc0000244.1_g000016.1.cds

Sequence Viewer

Length: 945 bp
atggctcagtttcaaagcaccagaatgaaaaaaagacatgaactggttcccaatgcaaaggctgggagggaatcgacagatattgaaatatatgggatgcaaggaatcccacctgacgtcttagctgcacattatggagaggaagaagacgacaccccgtcaaaaatggctaaagtggaaatcccatcaaatcagcttgttagcagtgtggtgccaggttcattggggacatatcctccccaaccacctgttggaatgcggccaatatacaattcatcgattccaatgcctcataatggttggcaagttgcacctcatccccagccttggtatccacagcctgtggcagcctctgttcctccgccttccggaatgggttattcacaacagccattgtttcctgtgcagccacctctaccatcaacaacatcccctggacttcaatcttcacatgtaactcctcctggactgcctgcatctatgcatcctgtaccagtatcacaacctctgtttcctgttgttggtgtcaataatttaccaactcaaagttctccattttctgctcccatgctttcaacaaataatccatcaaaccctctaggcttgatggatgcacatcagggcgctagaacttcgattccaaatagttaccatgcatcaaatattccaggtgggaacttcaataattcacactcttatgcatctggtccaaatactggtggcccatcaatcggaccaccccctgtaatagctaacaaagctccccaaccagccactaatgaggtctatttagtttgggatgatgaggctatgtccatggaggaaagaagaatggccttagcgaagtatcaggtgcatgatgaaactacccagatgagctcaattgatgcagccatagacagacggattttggagagcaggcttgctggtcgaatggcattttag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000070 GO:0000075 GO:0000226 GO:0000278 GO:0000280 GO:0000775 GO:0000776 GO:0000819 GO:0001578 GO:0003674 GO:0003700 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005694 GO:0005730 GO:0005856 GO:0006355 GO:0006996 GO:0007010 GO:0007017 GO:0007049 GO:0007051 GO:0007052 GO:0007059 GO:0007088 GO:0007093 GO:0007094 GO:0007346 GO:0008017 GO:0008092 GO:0008150 GO:0008270 GO:0008608 GO:0009889 GO:0009987 GO:0010468 GO:0010556 GO:0010564 GO:0010639 GO:0010948 GO:0010965 GO:0015631 GO:0016043 GO:0019219 GO:0019222 GO:0022402 GO:0022607 GO:0030071 GO:0031109 GO:0031323 GO:0031326 GO:0031577 GO:0031647 GO:0031974 GO:0031981 GO:0032991 GO:0033043 GO:0033044 GO:0033045 GO:0033046 GO:0033047 GO:0033048 GO:0034622 GO:0043167 GO:0043169 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043933 GO:0044085 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044430 GO:0044446 GO:0044464 GO:0045786 GO:0045839 GO:0045841 GO:0045930 GO:0046785 GO:0046872 GO:0046914 GO:0048285 GO:0048519 GO:0048523 GO:0050789 GO:0050794 GO:0050821 GO:0051128 GO:0051129 GO:0051171 GO:0051225 GO:0051252 GO:0051258 GO:0051276 GO:0051726 GO:0051783 GO:0051784 GO:0051983 GO:0051985 GO:0060255 GO:0065003 GO:0065007 GO:0065008 GO:0070013 GO:0070925 GO:0071173 GO:0071174 GO:0071840 GO:0080090 GO:0090307 GO:0097435 GO:0098687 GO:0098813 GO:0140014 GO:0140110 GO:1901987 GO:1901988 GO:1901990 GO:1901991 GO:1902099 GO:1902100 GO:1902850 GO:1903047 GO:1903506 GO:1905818 GO:1905819 GO:1990047 GO:2000112 GO:2000816 GO:2001141 GO:2001251
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

314

Amino Acids

33.67

Weight (kDa)

6.04

Isoelectric Point (pI)

72.4

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 120
AccB1I GGYRCC 1 cut(s) 211
AccB7I CCANNNNNTGG 2 cut(s) 251, 716
AccIII TCCGGA 1 cut(s) 368
AciI CCGC 2 cut(s) 259, 362
AcoI YGGCCR 1 cut(s) 260
AcyI GRCGYC 1 cut(s) 117
AfaI GTAC 1 cut(s) 492
AfiI CCNNNNNNNGG 7 cut(s) 251, 296, 327, 368, 521, 716, 731
AflIII ACRYGT 1 cut(s) 451
AgsI TTSAA 5 cut(s) 14, 86, 443, 576, 682
AhdI GACNNNNNGTC 1 cut(s) 157
AjnI CCWGG 4 cut(s) 214, 433, 463, 667
AjuI GAANNNNNNNTTGG 4 cut(s) 256, 288, 532, 564
AloI GAACNNNNNNTCC 2 cut(s) 622, 654
AluBI AGCT 5 cut(s) 125, 196, 752, 761, 879
AluI AGCT 5 cut(s) 125, 196, 752, 761, 879
Alw21I GWGCWC 1 cut(s) 881
AlwNI CAGNNNCTG 1 cut(s) 353
Aor13HI TCCGGA 1 cut(s) 368
AoxI GGCC 3 cut(s) 260, 721, 834
ApeKI GCWGC 4 cut(s) 125, 347, 406, 890
Asp700I GAANNNNTTC 1 cut(s) 45
AspLEI GCGC 1 cut(s) 626
AspS9I GGNCC 3 cut(s) 707, 722, 734
AvaII GGWCC 2 cut(s) 707, 734
BanI GGYRCC 1 cut(s) 211
BanII GRGCYC 1 cut(s) 881
BbsI GAAGAC 1 cut(s) 153
Bbv12I GWGCWC 1 cut(s) 881
BbvI GCAGC 4 cut(s) 112, 359, 418, 902
BccI CCATC 5 cut(s) 193, 427, 595, 601, 733
BcgI CGANNNNNNTGC 2 cut(s) 268, 302
BciT130I CCWGG 4 cut(s) 216, 435, 465, 669
BciVI GTATCC 1 cut(s) 342
BfaI CTAG 2 cut(s) 599, 627
BfoI RGCGCY 1 cut(s) 627
BfuI GTATCC 1 cut(s) 342
BisI GCNGC 5 cut(s) 126, 260, 348, 407, 891
BlsI GCNGC 5 cut(s) 127, 261, 349, 408, 892
Bme1390I CCNGG 4 cut(s) 216, 435, 465, 669
Bme18I GGWCC 2 cut(s) 707, 734
BmeRI GACNNNNNGTC 1 cut(s) 157
BmgT120I GGNCC 3 cut(s) 707, 722, 734
BmiI GGNNCC 2 cut(s) 48, 213
BmrFI CCNGG 4 cut(s) 216, 435, 465, 669
BmsI GCATC 7 cut(s) 87, 485, 493, 601, 665, 710, 877
BpiI GAAGAC 1 cut(s) 153
Bpu10I CCTNAGC 1 cut(s) 838
Bsa29I ATCGAT 1 cut(s) 278
BsaBI GATNNNNATC 1 cut(s) 615
BsaHI GRCGYC 1 cut(s) 117
BsaJI CCNNGG 3 cut(s) 326, 433, 816
BsaWI WCCGGW 1 cut(s) 368
BsaXI ACNNNNNCTCC 2 cut(s) 220, 250
Bsc4I CCNNNNNNNGG 7 cut(s) 251, 296, 327, 368, 521, 716, 731
Bse1I ACTGG 3 cut(s) 48, 494, 721
Bse8I GATNNNNATC 1 cut(s) 615
BseAI TCCGGA 1 cut(s) 368
BseBI CCWGG 4 cut(s) 216, 435, 465, 669
BseCI ATCGAT 1 cut(s) 278
BseDI CCNNGG 3 cut(s) 326, 433, 816
BseGI GGATG 6 cut(s) 102, 316, 428, 484, 616, 805
BseJI GATNNNNATC 1 cut(s) 615
BseLI CCNNNNNNNGG 7 cut(s) 251, 296, 327, 368, 521, 716, 731
BseMII CTCAG 1 cut(s) 20
BseNI ACTGG 3 cut(s) 48, 494, 721
BseRI GAGGAG 1 cut(s) 450
BseXI GCAGC 4 cut(s) 112, 359, 418, 902
BseYI CCCAGC 2 cut(s) 62, 321
BsgI GTGCAG 2 cut(s) 111, 425
BshFI GGCC 3 cut(s) 262, 723, 836
BshNI GGYRCC 1 cut(s) 211
BshVI ATCGAT 1 cut(s) 278
BsiHKAI GWGCWC 1 cut(s) 881
BsiSI CCGG 1 cut(s) 369
BslFI GGGAC 1 cut(s) 241
BslI CCNNNNNNNGG 7 cut(s) 251, 296, 327, 368, 521, 716, 731
BsmFI GGGAC 1 cut(s) 241
BsmI GAATGC 1 cut(s) 261
BsnI GGCC 3 cut(s) 262, 723, 836
Bsp1286I GDGCHC 1 cut(s) 881
Bsp13I TCCGGA 1 cut(s) 368
Bsp19I CCATGG 1 cut(s) 816
BspACI CCGC 2 cut(s) 259, 362
BspANI GGCC 3 cut(s) 262, 723, 836
BspCNI CTCAG 1 cut(s) 19
BspDI ATCGAT 1 cut(s) 278
BspEI TCCGGA 1 cut(s) 368
BspLI GGNNCC 2 cut(s) 48, 213
BspT107I GGYRCC 1 cut(s) 211
BsrI ACTGG 3 cut(s) 48, 494, 721
BssECI CCNNGG 3 cut(s) 326, 433, 816
BssNI GRCGYC 1 cut(s) 117
BssT1I CCWWGG 2 cut(s) 326, 816
Bst2UI CCWGG 4 cut(s) 216, 435, 465, 669
BstACI GRCGYC 1 cut(s) 117
BstC8I GCNNGC 3 cut(s) 474, 920, 924
BstDEI CTNAG 3 cut(s) 6, 121, 838
BstDSI CCRYGG 1 cut(s) 816
BstF5I GGATG 6 cut(s) 102, 316, 428, 484, 616, 805
BstH2I RGCGCY 1 cut(s) 627
BstHHI GCGC 1 cut(s) 626
BstMWI GCNNNNNNNGC 1 cut(s) 758
BstNI CCWGG 4 cut(s) 216, 435, 465, 669
BstNSI RCATGY 1 cut(s) 455
BstSCI CCNGG 4 cut(s) 214, 433, 463, 667
BstV1I GCAGC 4 cut(s) 112, 359, 418, 902
BstV2I GAAGAC 1 cut(s) 153
Bsu15I ATCGAT 1 cut(s) 278
BsuI GTATCC 1 cut(s) 342
BsuRI GGCC 3 cut(s) 262, 723, 836
BsuTUI ATCGAT 1 cut(s) 278
BtgI CCRYGG 1 cut(s) 816
BtsCI GGATG 6 cut(s) 102, 316, 428, 484, 616, 805
BtsI GCAGTG 1 cut(s) 211
BtsIMutI CAGTG 1 cut(s) 211
Cac8I GCNNGC 3 cut(s) 474, 920, 924
CaiI CAGNNNCTG 1 cut(s) 353
CfoI GCGC 1 cut(s) 626
Cfr13I GGNCC 3 cut(s) 707, 722, 734
ClaI ATCGAT 1 cut(s) 278
Csp6I GTAC 1 cut(s) 491
CviAII CATG 6 cut(s) 38, 452, 568, 653, 817, 857
CviQI GTAC 1 cut(s) 491
DdeI CTNAG 3 cut(s) 6, 121, 838
DriI GACNNNNNGTC 1 cut(s) 157
EaeI YGGCCR 1 cut(s) 260
Eam1105I GACNNNNNGTC 1 cut(s) 157
EciI GGCGGA 1 cut(s) 351
Ecl136II GAGCTC 1 cut(s) 879
Eco130I CCWWGG 2 cut(s) 326, 816
Eco24I GRGCYC 1 cut(s) 881
Eco47I GGWCC 2 cut(s) 707, 734
Eco53kI GAGCTC 1 cut(s) 879
EcoICRI GAGCTC 1 cut(s) 879
EcoRII CCWGG 4 cut(s) 214, 433, 463, 667
EcoT14I CCWWGG 2 cut(s) 326, 816
EcoT22I ATGCAT 3 cut(s) 486, 658, 703
EcoT38I GRGCYC 1 cut(s) 881
ErhI CCWWGG 2 cut(s) 326, 816
FaeI CATG 6 cut(s) 41, 455, 571, 656, 820, 860
FaqI GGGAC 1 cut(s) 241
FatI CATG 6 cut(s) 37, 451, 567, 652, 816, 856
Fnu4HI GCNGC 5 cut(s) 126, 260, 348, 407, 891
FokI GGATG 6 cut(s) 109, 303, 415, 471, 623, 812
FriOI GRGCYC 1 cut(s) 881
Fsp4HI GCNGC 5 cut(s) 126, 260, 348, 407, 891
FspBI CTAG 2 cut(s) 599, 627
GlaI GCGC 1 cut(s) 625
GluI GCNGC 5 cut(s) 126, 260, 348, 407, 891
GsaI CCCAGC 2 cut(s) 66, 325
HaeII RGCGCY 1 cut(s) 627
HaeIII GGCC 3 cut(s) 262, 723, 836
HapII CCGG 1 cut(s) 369
HhaI GCGC 1 cut(s) 626
Hin1I GRCGYC 1 cut(s) 117
Hin1II CATG 6 cut(s) 41, 455, 571, 656, 820, 860
Hin6I GCGC 1 cut(s) 624
HinP1I GCGC 1 cut(s) 624
HinfI GANTC 4 cut(s) 71, 105, 280, 637
HpaII CCGG 1 cut(s) 369
Hpy188I TCNGA 1 cut(s) 734
Hpy188III TCNNGA 1 cut(s) 369
HpyAV CCTTC 1 cut(s) 375
HpyCH4IV ACGT 1 cut(s) 117
HpyF10VI GCNNNNNNNGC 1 cut(s) 758
HpyF3I CTNAG 3 cut(s) 6, 121, 838
HpySE526I ACGT 1 cut(s) 117
Hsp92I GRCGYC 1 cut(s) 117
Hsp92II CATG 6 cut(s) 41, 455, 571, 656, 820, 860
HspAI GCGC 1 cut(s) 624
Kpn2I TCCGGA 1 cut(s) 368
LmnI GCTCC 2 cut(s) 568, 766
Lsp1109I GCAGC 4 cut(s) 112, 359, 418, 902
LweI GCATC 7 cut(s) 87, 485, 493, 601, 665, 710, 877
MaeI CTAG 2 cut(s) 599, 627
MaeII ACGT 1 cut(s) 117
MaeIII GTNAC 2 cut(s) 454, 647
MboII GAAGA 4 cut(s) 155, 158, 438, 840
MfeI CAATTG 1 cut(s) 882
MhlI GDGCHC 1 cut(s) 881
MluCI AATT 4 cut(s) 271, 532, 685, 882
MmeI TCCRAC 1 cut(s) 232
Mph1103I ATGCAT 3 cut(s) 486, 658, 703
MroI TCCGGA 1 cut(s) 368
MroXI GAANNNNTTC 1 cut(s) 45
MslI CAYNNNNRTG 2 cut(s) 23, 696
MspI CCGG 1 cut(s) 369
MspR9I CCNGG 4 cut(s) 216, 435, 465, 669
MunI CAATTG 1 cut(s) 882
Mva1269I GAATGC 1 cut(s) 261
MvaI CCWGG 4 cut(s) 216, 435, 465, 669
MwoI GCNNNNNNNGC 1 cut(s) 758
NcoI CCATGG 1 cut(s) 816
NlaIII CATG 6 cut(s) 41, 455, 571, 656, 820, 860
NlaIV GGNNCC 2 cut(s) 48, 213
NsiI ATGCAT 3 cut(s) 486, 658, 703
NspI RCATGY 1 cut(s) 455
PciI ACATGT 1 cut(s) 451
PctI GAATGC 1 cut(s) 261
PdmI GAANNNNTTC 1 cut(s) 45
PfeI GAWTC 4 cut(s) 71, 105, 280, 637
PflMI CCANNNNNTGG 2 cut(s) 251, 716
PfoI TCCNGGA 1 cut(s) 463
PkrI GCNGC 5 cut(s) 127, 261, 349, 408, 892
PscI ACATGT 1 cut(s) 451
Psp124BI GAGCTC 1 cut(s) 881
Psp6I CCWGG 4 cut(s) 214, 433, 463, 667
PspFI CCCAGC 2 cut(s) 62, 321
PspGI CCWGG 4 cut(s) 214, 433, 463, 667
PspN4I GGNNCC 2 cut(s) 48, 213
PspPI GGNCC 3 cut(s) 707, 722, 734
PstNI CAGNNNCTG 1 cut(s) 353
RsaI GTAC 1 cut(s) 492
RsaNI GTAC 1 cut(s) 491
RseI CAYNNNNRTG 2 cut(s) 23, 696
SacI GAGCTC 1 cut(s) 881
SatI GCNGC 5 cut(s) 126, 260, 348, 407, 891
Sau96I GGNCC 3 cut(s) 707, 722, 734
ScrFI CCNGG 4 cut(s) 216, 435, 465, 669
SduI GDGCHC 1 cut(s) 881
SfaNI GCATC 7 cut(s) 87, 485, 493, 601, 665, 710, 877
SinI GGWCC 2 cut(s) 707, 734
SmiMI CAYNNNNRTG 2 cut(s) 23, 696
Sse9I AATT 4 cut(s) 271, 532, 685, 882
SsiI CCGC 2 cut(s) 259, 362
SspI AATATT 1 cut(s) 664
SspMI CTAG 2 cut(s) 599, 627
SstI GAGCTC 1 cut(s) 881
StyD4I CCNGG 4 cut(s) 214, 433, 463, 667
StyI CCWWGG 2 cut(s) 326, 816
TaiI ACGT 1 cut(s) 120
TaqI TCGA 4 cut(s) 74, 278, 635, 931
TasI AATT 4 cut(s) 271, 532, 685, 882
TauI GCSGC 1 cut(s) 262
TfiI GAWTC 4 cut(s) 71, 105, 280, 637
TscAI CASTG 1 cut(s) 211
TseI GCWGC 4 cut(s) 125, 347, 406, 890
TspDTI ATGAA 5 cut(s) 41, 54, 210, 264, 876
TspGWI ACGGA 1 cut(s) 919
TspRI CASTG 1 cut(s) 211
Van91I CCANNNNNTGG 2 cut(s) 251, 716
VpaK11BI GGWCC 2 cut(s) 707, 734
XceI RCATGY 1 cut(s) 455
XcmI CCANNNNNNNNNTGG 1 cut(s) 248
XmnI GAANNNNTTC 1 cut(s) 45
XspI CTAG 2 cut(s) 599, 627
ZraI GACGTC 1 cut(s) 118
Zsp2I ATGCAT 3 cut(s) 486, 658, 703
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.