Rh5BG153200

Protein SUPPRESSOR OF FRI

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5B
OG0006315
Physical Location & Seq
Forward (+)
15676931 .. 15681730
4800 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5BG153200.1

Sequence Viewer

Length: 939 bp
ATGGGAAAGAAGAAGAAGAGGGTGGCCTCGAAGGTGTGGTGTTACTACTGCGATAGGGAGTTTGACGACGAGAAGATATTGGTGCAGCACCAGAAAGCTAAGCACTTTAAGTGCCATGTCTGCCACAAGAAGCTTTCTACAGCTGGGGGTATGGCCATTCATGTCCTCCAGGTCCACAAAGAGAACGTCACCAAGGTTCCCAATGCAAAGGCTGGGAGGGAATCGACAGATATTGAAATATATGGGATGCAAGGAATCCCACCTGACGTCTTAGCTGCACATTATGGAGAGGAAGAAGACGACGCCCCGTCAAAAATGGCTAAAGTGGAAATCCCATCAACTCAGCTTGTTAGCAGTGTGGTGCCAGGTTCATTGGGGACATATCCTCCCCAACCACCTTTTGGAATGCGGCCAATATACAATTCATCGATCCCAATGCCTCATAACGGTTGGCAAGTTGCACCTCGTCCCCAGCCTTGGTATCCACAGCCTCTGGCAGTCTCTGTTCCTCCGCCTTCCGGAATGGGTTATTCACAACAGCCATTGTTTCCTGTGCAGCCACCTCTACCATCAACAACATCCCCTGGACTTCAATCTTCACATGTAACTCCTCCTGGACTGCCTGCATCTATGCATCCTGTACCTGTATCACAACCTCTGTTTCCTGTTGTTGGTGTCAATAATTTACCAACTCAAAGTTCTCCATTTTCTGCTCCCATGCTTTCAACAAATAATCCATCAAACCCTCTAGGCTCGATGGATGCACATCAGGGCGCTAGAACTTCGATTCCAAATAGTTACCATGCTTCAAATATTCCAGGTTTGATTTCTTTTTATTGTATTATTTCTACAGTGATTCAAATCTGGTTATGCTACAAAATAATTGTTGTTTTTTTTTTTTTTTTTTTTTTTTTTTTCATTTTTGTTCTTTCAAAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000070 GO:0000075 GO:0000226 GO:0000278 GO:0000280 GO:0000775 GO:0000776 GO:0000819 GO:0001578 GO:0003674 GO:0003700 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005694 GO:0005730 GO:0005856 GO:0006355 GO:0006996 GO:0007010 GO:0007017 GO:0007049 GO:0007051 GO:0007052 GO:0007059 GO:0007088 GO:0007093 GO:0007094 GO:0007346 GO:0008017 GO:0008092 GO:0008150 GO:0008270 GO:0008608 GO:0009889 GO:0009987 GO:0010468 GO:0010556 GO:0010564 GO:0010639 GO:0010948 GO:0010965 GO:0015631 GO:0016043 GO:0019219 GO:0019222 GO:0022402 GO:0022607 GO:0030071 GO:0031109 GO:0031323 GO:0031326 GO:0031577 GO:0031647 GO:0031974 GO:0031981 GO:0032991 GO:0033043 GO:0033044 GO:0033045 GO:0033046 GO:0033047 GO:0033048 GO:0034622 GO:0043167 GO:0043169 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043933 GO:0044085 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044430 GO:0044446 GO:0044464 GO:0045786 GO:0045839 GO:0045841 GO:0045930 GO:0046785 GO:0046872 GO:0046914 GO:0048285 GO:0048519 GO:0048523 GO:0050789 GO:0050794 GO:0050821 GO:0051128 GO:0051129 GO:0051171 GO:0051225 GO:0051252 GO:0051258 GO:0051276 GO:0051726 GO:0051783 GO:0051784 GO:0051983 GO:0051985 GO:0060255 GO:0065003 GO:0065007 GO:0065008 GO:0070013 GO:0070925 GO:0071173 GO:0071174 GO:0071840 GO:0080090 GO:0090307 GO:0097435 GO:0098687 GO:0098813 GO:0140014 GO:0140110 GO:1901987 GO:1901988 GO:1901990 GO:1901991 GO:1902099 GO:1902100 GO:1902850 GO:1903047 GO:1903506 GO:1905818 GO:1905819 GO:1990047 GO:2000112 GO:2000816 GO:2001141 GO:2001251
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

312

Amino Acids

34.33

Weight (kDa)

9.04

Isoelectric Point (pI)

62.38

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 270
AccB1I GGYRCC 1 cut(s) 361
AccB7I CCANNNNNTGG 1 cut(s) 401
AccIII TCCGGA 1 cut(s) 518
AciI CCGC 2 cut(s) 409, 512
AclWI GGATC 1 cut(s) 424
AcoI YGGCCR 2 cut(s) 153, 410
AcyI GRCGYC 2 cut(s) 267, 303
AfaI GTAC 1 cut(s) 642
AfiI CCNNNNNNNGG 5 cut(s) 401, 446, 477, 518, 671
AflIII ACRYGT 1 cut(s) 601
AgsI TTSAA 6 cut(s) 236, 593, 726, 810, 860, 933
AhdI GACNNNNNGTC 1 cut(s) 307
AjnI CCWGG 5 cut(s) 168, 364, 583, 613, 817
AjuI GAANNNNNNNTTGG 4 cut(s) 406, 438, 682, 714
AloI GAACNNNNNNTCC 2 cut(s) 772, 804
AluBI AGCT 5 cut(s) 98, 133, 143, 275, 346
AluI AGCT 5 cut(s) 98, 133, 143, 275, 346
Alw26I GTCTC 1 cut(s) 505
AlwI GGATC 1 cut(s) 424
AlwNI CAGNNNCTG 2 cut(s) 493, 503
Aor13HI TCCGGA 1 cut(s) 518
AoxI GGCC 3 cut(s) 24, 153, 410
ApeKI GCWGC 3 cut(s) 85, 275, 556
ArsI GACNNNNNNTTYG 2 cut(s) 171, 203
AspLEI GCGC 1 cut(s) 776
AspS9I GGNCC 1 cut(s) 172
AsuHPI GGTGA 1 cut(s) 181
AvaII GGWCC 1 cut(s) 172
BalI TGGCCA 1 cut(s) 155
BanI GGYRCC 1 cut(s) 361
BbsI GAAGAC 1 cut(s) 303
BbvI GCAGC 3 cut(s) 97, 262, 568
BccI CCATC 4 cut(s) 343, 577, 745, 751
BcgI CGANNNNNNTGC 2 cut(s) 418, 452
BciT130I CCWGG 5 cut(s) 170, 366, 585, 615, 819
BciVI GTATCC 1 cut(s) 492
BcoDI GTCTC 1 cut(s) 505
BfaI CTAG 2 cut(s) 749, 777
BfmI CTRYAG 2 cut(s) 138, 849
BfoI RGCGCY 1 cut(s) 777
BfuI GTATCC 1 cut(s) 492
BisI GCNGC 4 cut(s) 86, 276, 410, 557
BlpI GCTNAGC 1 cut(s) 99
BlsI GCNGC 4 cut(s) 87, 277, 411, 558
Bme1390I CCNGG 5 cut(s) 170, 366, 585, 615, 819
Bme18I GGWCC 1 cut(s) 172
BmeRI GACNNNNNGTC 1 cut(s) 307
BmgT120I GGNCC 1 cut(s) 172
BmiI GGNNCC 2 cut(s) 198, 363
BmrFI CCNGG 5 cut(s) 170, 366, 585, 615, 819
BmsI GCATC 4 cut(s) 237, 635, 643, 751
BpiI GAAGAC 1 cut(s) 303
BpmI CTGGAG 1 cut(s) 152
Bpu1102I GCTNAGC 1 cut(s) 99
Bsa29I ATCGAT 1 cut(s) 428
BsaBI GATNNNNATC 2 cut(s) 765, 860
BsaHI GRCGYC 2 cut(s) 267, 303
BsaJI CCNNGG 3 cut(s) 192, 476, 583
BsaWI WCCGGW 1 cut(s) 518
BsaXI ACNNNNNCTCC 2 cut(s) 370, 400
Bsc4I CCNNNNNNNGG 5 cut(s) 401, 446, 477, 518, 671
Bse8I GATNNNNATC 2 cut(s) 765, 860
BseAI TCCGGA 1 cut(s) 518
BseBI CCWGG 5 cut(s) 170, 366, 585, 615, 819
BseCI ATCGAT 1 cut(s) 428
BseDI CCNNGG 3 cut(s) 192, 476, 583
BseGI GGATG 4 cut(s) 252, 578, 634, 766
BseJI GATNNNNATC 2 cut(s) 765, 860
BseLI CCNNNNNNNGG 5 cut(s) 401, 446, 477, 518, 671
BseMII CTCAG 1 cut(s) 356
BseRI GAGGAG 1 cut(s) 600
BseXI GCAGC 3 cut(s) 97, 262, 568
BseYI CCCAGC 3 cut(s) 143, 212, 471
BsgI GTGCAG 3 cut(s) 104, 261, 575
BshFI GGCC 3 cut(s) 26, 155, 412
BshNI GGYRCC 1 cut(s) 361
BshVI ATCGAT 1 cut(s) 428
BsiSI CCGG 1 cut(s) 519
BslFI GGGAC 2 cut(s) 391, 453
BslI CCNNNNNNNGG 5 cut(s) 401, 446, 477, 518, 671
BsmAI GTCTC 1 cut(s) 505
BsmFI GGGAC 2 cut(s) 391, 453
BsmI GAATGC 1 cut(s) 411
BsnI GGCC 3 cut(s) 26, 155, 412
Bsp13I TCCGGA 1 cut(s) 518
Bsp143I GATC 1 cut(s) 429
Bsp1720I GCTNAGC 1 cut(s) 99
BspACI CCGC 2 cut(s) 409, 512
BspANI GGCC 3 cut(s) 26, 155, 412
BspCNI CTCAG 1 cut(s) 355
BspDI ATCGAT 1 cut(s) 428
BspEI TCCGGA 1 cut(s) 518
BspLI GGNNCC 2 cut(s) 198, 363
BspPI GGATC 1 cut(s) 424
BspT107I GGYRCC 1 cut(s) 361
BssECI CCNNGG 3 cut(s) 192, 476, 583
BssMI GATC 1 cut(s) 429
BssNI GRCGYC 2 cut(s) 267, 303
BssT1I CCWWGG 2 cut(s) 192, 476
Bst2UI CCWGG 5 cut(s) 170, 366, 585, 615, 819
Bst4CI ACNGT 2 cut(s) 449, 853
Bst6I CTCTTC 1 cut(s) 11
BstACI GRCGYC 2 cut(s) 267, 303
BstC8I GCNNGC 1 cut(s) 624
BstDEI CTNAG 3 cut(s) 99, 271, 342
BstF5I GGATG 4 cut(s) 252, 578, 634, 766
BstH2I RGCGCY 1 cut(s) 777
BstHHI GCGC 1 cut(s) 776
BstKTI GATC 1 cut(s) 432
BstMAI GTCTC 1 cut(s) 505
BstMBI GATC 1 cut(s) 429
BstMWI GCNNNNNNNGC 1 cut(s) 120
BstNI CCWGG 5 cut(s) 170, 366, 585, 615, 819
BstNSI RCATGY 1 cut(s) 605
BstSCI CCNGG 5 cut(s) 168, 364, 583, 613, 817
BstSFI CTRYAG 2 cut(s) 138, 849
BstV1I GCAGC 3 cut(s) 97, 262, 568
BstV2I GAAGAC 1 cut(s) 303
Bsu15I ATCGAT 1 cut(s) 428
BsuI GTATCC 1 cut(s) 492
BsuRI GGCC 3 cut(s) 26, 155, 412
BsuTUI ATCGAT 1 cut(s) 428
BtsCI GGATG 4 cut(s) 252, 578, 634, 766
BtsI GCAGTG 1 cut(s) 361
BtsIMutI CAGTG 2 cut(s) 361, 858
Cac8I GCNNGC 1 cut(s) 624
CaiI CAGNNNCTG 2 cut(s) 493, 503
CfoI GCGC 1 cut(s) 776
Cfr13I GGNCC 1 cut(s) 172
ClaI ATCGAT 1 cut(s) 428
CseI GACGC 1 cut(s) 311
Csp6I GTAC 1 cut(s) 641
CviAII CATG 5 cut(s) 116, 161, 602, 718, 803
CviQI GTAC 1 cut(s) 641
DdeI CTNAG 3 cut(s) 99, 271, 342
DpnI GATC 1 cut(s) 431
DpnII GATC 1 cut(s) 429
DriI GACNNNNNGTC 1 cut(s) 307
EaeI YGGCCR 2 cut(s) 153, 410
Eam1104I CTCTTC 1 cut(s) 11
Eam1105I GACNNNNNGTC 1 cut(s) 307
EarI CTCTTC 1 cut(s) 11
EciI GGCGGA 1 cut(s) 501
Eco130I CCWWGG 2 cut(s) 192, 476
Eco47I GGWCC 1 cut(s) 172
EcoRII CCWGG 5 cut(s) 168, 364, 583, 613, 817
EcoT14I CCWWGG 2 cut(s) 192, 476
EcoT22I ATGCAT 1 cut(s) 636
ErhI CCWWGG 2 cut(s) 192, 476
FaeI CATG 5 cut(s) 119, 164, 605, 721, 806
FaqI GGGAC 2 cut(s) 391, 453
FatI CATG 5 cut(s) 115, 160, 601, 717, 802
Fnu4HI GCNGC 4 cut(s) 86, 276, 410, 557
FokI GGATG 4 cut(s) 259, 565, 621, 773
Fsp4HI GCNGC 4 cut(s) 86, 276, 410, 557
FspBI CTAG 2 cut(s) 749, 777
GlaI GCGC 1 cut(s) 775
GluI GCNGC 4 cut(s) 86, 276, 410, 557
GsaI CCCAGC 3 cut(s) 147, 216, 475
GsuI CTGGAG 1 cut(s) 152
HaeII RGCGCY 1 cut(s) 777
HaeIII GGCC 3 cut(s) 26, 155, 412
HapII CCGG 1 cut(s) 519
HgaI GACGC 1 cut(s) 311
HhaI GCGC 1 cut(s) 776
Hin1I GRCGYC 2 cut(s) 267, 303
Hin1II CATG 5 cut(s) 119, 164, 605, 721, 806
Hin6I GCGC 1 cut(s) 774
HinP1I GCGC 1 cut(s) 774
HindIII AAGCTT 1 cut(s) 131
HinfI GANTC 4 cut(s) 221, 255, 787, 856
HpaII CCGG 1 cut(s) 519
HphI GGTGA 1 cut(s) 181
Hpy166II GTNNAC 1 cut(s) 175
Hpy188III TCNNGA 1 cut(s) 519
Hpy8I GTNNAC 1 cut(s) 175
Hpy99I CGWCG 2 cut(s) 71, 305
HpyAV CCTTC 2 cut(s) 25, 525
HpyCH4III ACNGT 2 cut(s) 449, 853
HpyCH4IV ACGT 2 cut(s) 186, 267
HpyCH4V TGCA 9 cut(s) 85, 206, 250, 278, 461, 556, 626, 634, 764
HpyF10VI GCNNNNNNNGC 1 cut(s) 120
HpyF3I CTNAG 3 cut(s) 99, 271, 342
HpySE526I ACGT 2 cut(s) 186, 267
Hsp92I GRCGYC 2 cut(s) 267, 303
Hsp92II CATG 5 cut(s) 119, 164, 605, 721, 806
HspAI GCGC 1 cut(s) 774
Kpn2I TCCGGA 1 cut(s) 518
Kzo9I GATC 1 cut(s) 429
LmnI GCTCC 1 cut(s) 718
Lsp1109I GCAGC 3 cut(s) 97, 262, 568
LweI GCATC 4 cut(s) 237, 635, 643, 751
MaeI CTAG 2 cut(s) 749, 777
MaeII ACGT 2 cut(s) 186, 267
MaeIII GTNAC 4 cut(s) 41, 187, 604, 797
MalI GATC 1 cut(s) 431
MboI GATC 1 cut(s) 429
MboII GAAGA 7 cut(s) 22, 25, 28, 85, 305, 308, 588
MlsI TGGCCA 1 cut(s) 155
MluCI AATT 3 cut(s) 421, 682, 882
MluNI TGGCCA 1 cut(s) 155
Mox20I TGGCCA 1 cut(s) 155
Mph1103I ATGCAT 1 cut(s) 636
MroI TCCGGA 1 cut(s) 518
MscI TGGCCA 1 cut(s) 155
MseI TTAA 1 cut(s) 108
Msp20I TGGCCA 1 cut(s) 155
MspA1I CMGCKG 1 cut(s) 143
MspI CCGG 1 cut(s) 519
MspR9I CCNGG 5 cut(s) 170, 366, 585, 615, 819
Mva1269I GAATGC 1 cut(s) 411
MvaI CCWGG 5 cut(s) 170, 366, 585, 615, 819
MwoI GCNNNNNNNGC 1 cut(s) 120
NdeII GATC 1 cut(s) 429
NlaIII CATG 5 cut(s) 119, 164, 605, 721, 806
NlaIV GGNNCC 2 cut(s) 198, 363
NmuCI GTSAC 1 cut(s) 187
NsiI ATGCAT 1 cut(s) 636
NspI RCATGY 1 cut(s) 605
PciI ACATGT 1 cut(s) 601
PctI GAATGC 1 cut(s) 411
PfeI GAWTC 4 cut(s) 221, 255, 787, 856
PflMI CCANNNNNTGG 1 cut(s) 401
PfoI TCCNGGA 1 cut(s) 613
PkrI GCNGC 4 cut(s) 87, 277, 411, 558
PscI ACATGT 1 cut(s) 601
Psp6I CCWGG 5 cut(s) 168, 364, 583, 613, 817
PspFI CCCAGC 3 cut(s) 143, 212, 471
PspGI CCWGG 5 cut(s) 168, 364, 583, 613, 817
PspN4I GGNNCC 2 cut(s) 198, 363
PspPI GGNCC 1 cut(s) 172
PstNI CAGNNNCTG 2 cut(s) 493, 503
PvuII CAGCTG 1 cut(s) 143
RsaI GTAC 1 cut(s) 642
RsaNI GTAC 1 cut(s) 641
SaqAI TTAA 1 cut(s) 108
SatI GCNGC 4 cut(s) 86, 276, 410, 557
Sau3AI GATC 1 cut(s) 429
Sau96I GGNCC 1 cut(s) 172
ScrFI CCNGG 5 cut(s) 170, 366, 585, 615, 819
SfaNI GCATC 4 cut(s) 237, 635, 643, 751
SfcI CTRYAG 2 cut(s) 138, 849
SinI GGWCC 1 cut(s) 172
Sse9I AATT 3 cut(s) 421, 682, 882
SsiI CCGC 2 cut(s) 409, 512
SspI AATATT 1 cut(s) 814
SspMI CTAG 2 cut(s) 749, 777
StyD4I CCNGG 5 cut(s) 168, 364, 583, 613, 817
StyI CCWWGG 2 cut(s) 192, 476
TaaI ACNGT 2 cut(s) 449, 853
TaiI ACGT 2 cut(s) 189, 270
TaqI TCGA 5 cut(s) 29, 224, 428, 755, 785
TasI AATT 3 cut(s) 421, 682, 882
TauI GCSGC 1 cut(s) 412
TfiI GAWTC 4 cut(s) 221, 255, 787, 856
Tru1I TTAA 1 cut(s) 108
Tru9I TTAA 1 cut(s) 108
TscAI CASTG 2 cut(s) 361, 858
TseFI GTSAC 1 cut(s) 187
TseI GCWGC 3 cut(s) 85, 275, 556
Tsp45I GTSAC 1 cut(s) 187
TspDTI ATGAA 4 cut(s) 149, 360, 414, 907
TspRI CASTG 2 cut(s) 361, 858
Van91I CCANNNNNTGG 1 cut(s) 401
VpaK11BI GGWCC 1 cut(s) 172
XceI RCATGY 1 cut(s) 605
XcmI CCANNNNNNNNNTGG 1 cut(s) 398
XspI CTAG 2 cut(s) 749, 777
ZraI GACGTC 1 cut(s) 268
Zsp2I ATGCAT 1 cut(s) 636
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.