Prupe.4G117400_v2.0.a1

Protein SUPPRESSOR OF FRI

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp04
OG0006315
Physical Location & Seq
Forward (+)
6350829 .. 6355875
5047 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.4G117400.2

Sequence Viewer

Length: 1071 bp
ATGGGTAGGAAGAAGAAGAGGGTTTCGTCGAAGGTTTGGTGCTACTACTGTGATAGAGAATTTGACGATGAGAAGATACTGGTGCAGCACCAGAAAGCCAAGCACTTTAAGTGCCATGTCTGCCACAAGAAGCTATCTACCGCTGGTGGCATGGCCATTCATGTCCTCCAGGTCCACAAAGAGAGCGTCACCAAAGTTCCCAATGCAAAGACTGGAAGAGAGTCAACGGATATTGAAATATATGGGATGCAAGGAATCCCACCTGATGTCTTGGCTGCACATTATGGAGAGGAAGATGAGGACGCTCCATCAAAAGTAGCTAAAGTGGACATCCCGTCAACCCAGTTTGTTGGTGGTATGGTGCCAGGTTCGATGGGGATTGGATATCCTCCTCAACCAGCTTTGGGTGCAATCCGGCCAATTTACAGTCCTGCAGTTCCAATGCCTCCAAATGCTTGGCAACCTCGTCCCCAGCCATGGTTTCCACAACTTCCAGCAGTCTCAATTCCTCCTCCTTCCTCATTGGGTTATGTGCCGCAGCCATTGTTTCCTGTGCAGAATGTGAGGCCACCCCCACCATCATCAACCCCTGCACATCTGCCGTCACATATAACTCCGCCTGGACTTCCTACATCCATGCCTCCTGTTCCTGTATCACAACCCCTGTTTCCTGTTGTTGGTATAAATAATGTACCAACTCAAAGTTCTCCCTTTTCAGCTCCTATGCCTTCAACTAGTATTCTCTCAAATTCTCCAGCTGAACTTAAAGGCTCAACAGATGCGCATCAAGGTGGGACCTTAATTAATTCACACTCTTATGCATCTGGTCCAAATACTGGTGGTCCATCAATTGGACCACCGCCTGTAATTGCAAACAAAGCTCCTGCTCCTCAGCCAGCAGCTAATGAGGTGTATCTGGTTTGGGATGATGAGGCTATGTCCATGGAGGAAAGAAGAATGTCCTTAGTGAAGTATCAGGTGCATGATGAAACTAGCCAGATGAGTTCAATTGATGCAGCCATAGACAGAAGGATTTTGGAGAGCCGGCTCGCTGGCCGGATGGCATTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000070 GO:0000075 GO:0000226 GO:0000278 GO:0000280 GO:0000775 GO:0000776 GO:0000819 GO:0001578 GO:0003674 GO:0003700 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005694 GO:0005730 GO:0005856 GO:0006355 GO:0006996 GO:0007010 GO:0007017 GO:0007049 GO:0007051 GO:0007052 GO:0007059 GO:0007088 GO:0007093 GO:0007094 GO:0007346 GO:0008017 GO:0008092 GO:0008150 GO:0008270 GO:0008608 GO:0009889 GO:0009987 GO:0010468 GO:0010556 GO:0010564 GO:0010639 GO:0010948 GO:0010965 GO:0015631 GO:0016043 GO:0019219 GO:0019222 GO:0022402 GO:0022607 GO:0030071 GO:0031109 GO:0031323 GO:0031326 GO:0031577 GO:0031647 GO:0031974 GO:0031981 GO:0032991 GO:0033043 GO:0033044 GO:0033045 GO:0033046 GO:0033047 GO:0033048 GO:0034622 GO:0043167 GO:0043169 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043933 GO:0044085 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044430 GO:0044446 GO:0044464 GO:0045786 GO:0045839 GO:0045841 GO:0045930 GO:0046785 GO:0046872 GO:0046914 GO:0048285 GO:0048519 GO:0048523 GO:0050789 GO:0050794 GO:0050821 GO:0051128 GO:0051129 GO:0051171 GO:0051225 GO:0051252 GO:0051258 GO:0051276 GO:0051726 GO:0051783 GO:0051784 GO:0051983 GO:0051985 GO:0060255 GO:0065003 GO:0065007 GO:0065008 GO:0070013 GO:0070925 GO:0071173 GO:0071174 GO:0071840 GO:0080090 GO:0090307 GO:0097435 GO:0098687 GO:0098813 GO:0140014 GO:0140110 GO:1901987 GO:1901988 GO:1901990 GO:1901991 GO:1902099 GO:1902100 GO:1902850 GO:1903047 GO:1903506 GO:1905818 GO:1905819 GO:1990047 GO:2000112 GO:2000816 GO:2001141 GO:2001251
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

357

Amino Acids

38.38

Weight (kDa)

8.26

Isoelectric Point (pI)

73.43

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 783
AccB1I GGYRCC 1 cut(s) 361
AccB7I CCANNNNNTGG 2 cut(s) 836, 851
AciI CCGC 4 cut(s) 141, 536, 617, 860
AcoI YGGCCR 3 cut(s) 153, 416, 1054
AcsI RAATTY 2 cut(s) 59, 748
AfaI GTAC 1 cut(s) 693
AfiI CCNNNNNNNGG 5 cut(s) 404, 477, 677, 836, 851
AgsI TTSAA 3 cut(s) 236, 732, 1008
AhdI GACNNNNNGTC 1 cut(s) 334
AhlI ACTAGT 1 cut(s) 734
AjnI CCWGG 3 cut(s) 168, 364, 619
AjuI GAANNNNNNNTTGG 2 cut(s) 688, 720
AluBI AGCT 7 cut(s) 133, 320, 401, 719, 758, 881, 902
AluI AGCT 7 cut(s) 133, 320, 401, 719, 758, 881, 902
Alw26I GTCTC 1 cut(s) 505
AoxI GGCC 4 cut(s) 153, 416, 566, 1054
ApeKI GCWGC 5 cut(s) 85, 275, 538, 899, 1016
ApoI RAATTY 2 cut(s) 59, 748
ArsI GACNNNNNNTTYG 2 cut(s) 171, 203
AseI ATTAAT 1 cut(s) 804
AspLEI GCGC 1 cut(s) 784
AspS9I GGNCC 5 cut(s) 172, 795, 827, 842, 854
AsuHPI GGTGA 1 cut(s) 181
AvaII GGWCC 5 cut(s) 172, 795, 827, 842, 854
BalI TGGCCA 1 cut(s) 155
BanI GGYRCC 1 cut(s) 361
BbvCI CCTCAGC 1 cut(s) 891
BbvI GCAGC 5 cut(s) 97, 262, 550, 911, 1028
BccI CCATC 5 cut(s) 316, 367, 586, 853, 1054
BceAI ACGGC 1 cut(s) 586
BciT130I CCWGG 3 cut(s) 170, 366, 621
BcoDI GTCTC 1 cut(s) 505
BcuI ACTAGT 1 cut(s) 734
BfaI CTAG 2 cut(s) 735, 993
BfmI CTRYAG 1 cut(s) 432
BisI GCNGC 6 cut(s) 86, 276, 536, 539, 900, 1017
BlsI GCNGC 6 cut(s) 87, 277, 537, 540, 901, 1018
Bme1390I CCNGG 3 cut(s) 170, 366, 621
Bme18I GGWCC 5 cut(s) 172, 795, 827, 842, 854
BmeRI GACNNNNNGTC 1 cut(s) 334
BmgT120I GGNCC 5 cut(s) 172, 795, 827, 842, 854
BmiI GGNNCC 2 cut(s) 363, 796
BmrFI CCNGG 3 cut(s) 170, 366, 621
BmrI ACTGGG 1 cut(s) 337
BmsI GCATC 5 cut(s) 237, 769, 793, 830, 1003
BmuI ACTGGG 1 cut(s) 337
BpmI CTGGAG 2 cut(s) 152, 738
Bpu10I CCTNAGC 1 cut(s) 891
BsaBI GATNNNNATC 1 cut(s) 783
BsaJI CCNNGG 2 cut(s) 476, 942
Bsc4I CCNNNNNNNGG 5 cut(s) 404, 477, 677, 836, 851
Bse118I RCCGGY 1 cut(s) 1044
Bse1I ACTGG 4 cut(s) 84, 217, 343, 841
Bse8I GATNNNNATC 1 cut(s) 783
BseBI CCWGG 3 cut(s) 170, 366, 621
BseDI CCNNGG 2 cut(s) 476, 942
BseGI GGATG 5 cut(s) 252, 330, 632, 931, 1065
BseJI GATNNNNATC 1 cut(s) 783
BseLI CCNNNNNNNGG 5 cut(s) 404, 477, 677, 836, 851
BseMII CTCAG 1 cut(s) 905
BseNI ACTGG 4 cut(s) 84, 217, 343, 841
BseRI GAGGAG 3 cut(s) 381, 501, 879
BseXI GCAGC 5 cut(s) 97, 262, 550, 911, 1028
BseYI CCCAGC 1 cut(s) 471
BsgI GTGCAG 4 cut(s) 104, 261, 575, 576
BshFI GGCC 4 cut(s) 155, 418, 568, 1056
BshNI GGYRCC 1 cut(s) 361
BsiSI CCGG 3 cut(s) 415, 1045, 1057
BslFI GGGAC 2 cut(s) 453, 808
BslI CCNNNNNNNGG 5 cut(s) 404, 477, 677, 836, 851
BsmAI GTCTC 1 cut(s) 505
BsmFI GGGAC 2 cut(s) 453, 808
BsnI GGCC 4 cut(s) 155, 418, 568, 1056
Bsp19I CCATGG 2 cut(s) 476, 942
BspACI CCGC 4 cut(s) 141, 536, 617, 860
BspANI GGCC 4 cut(s) 155, 418, 568, 1056
BspCNI CTCAG 1 cut(s) 904
BspLI GGNNCC 2 cut(s) 363, 796
BspMAI CTGCAG 1 cut(s) 436
BspT107I GGYRCC 1 cut(s) 361
BsrFI RCCGGY 1 cut(s) 1044
BsrI ACTGG 4 cut(s) 84, 217, 343, 841
BssAI RCCGGY 1 cut(s) 1044
BssECI CCNNGG 2 cut(s) 476, 942
BssT1I CCWWGG 2 cut(s) 476, 942
Bst2UI CCWGG 3 cut(s) 170, 366, 621
Bst4CI ACNGT 2 cut(s) 50, 428
Bst6I CTCTTC 2 cut(s) 11, 211
BstC8I GCNNGC 4 cut(s) 897, 1046, 1050, 1054
BstDEI CTNAG 2 cut(s) 891, 964
BstDSI CCRYGG 2 cut(s) 476, 942
BstF5I GGATG 5 cut(s) 252, 330, 632, 931, 1065
BstHHI GCGC 1 cut(s) 784
BstMAI GTCTC 1 cut(s) 505
BstMWI GCNNNNNNNGC 3 cut(s) 120, 407, 878
BstNI CCWGG 3 cut(s) 170, 366, 621
BstSCI CCNGG 3 cut(s) 168, 364, 619
BstSFI CTRYAG 1 cut(s) 432
BstV1I GCAGC 5 cut(s) 97, 262, 550, 911, 1028
BstXI CCANNNNNNTGG 2 cut(s) 350, 456
BsuRI GGCC 4 cut(s) 155, 418, 568, 1056
BtgI CCRYGG 2 cut(s) 476, 942
BtsCI GGATG 5 cut(s) 252, 330, 632, 931, 1065
Cac8I GCNNGC 4 cut(s) 897, 1046, 1050, 1054
CfoI GCGC 1 cut(s) 784
Cfr10I RCCGGY 1 cut(s) 1044
Cfr13I GGNCC 5 cut(s) 172, 795, 827, 842, 854
CseI GACGC 2 cut(s) 175, 311
Csp6I GTAC 1 cut(s) 692
CviAII CATG 7 cut(s) 116, 151, 161, 477, 637, 943, 983
CviQI GTAC 1 cut(s) 692
DdeI CTNAG 2 cut(s) 891, 964
DriI GACNNNNNGTC 1 cut(s) 334
EaeI YGGCCR 3 cut(s) 153, 416, 1054
Eam1104I CTCTTC 2 cut(s) 11, 211
Eam1105I GACNNNNNGTC 1 cut(s) 334
EarI CTCTTC 2 cut(s) 11, 211
EciI GGCGGA 1 cut(s) 606
Eco130I CCWWGG 2 cut(s) 476, 942
Eco32I GATATC 1 cut(s) 386
Eco47I GGWCC 5 cut(s) 172, 795, 827, 842, 854
EcoO109I RGGNCCY 1 cut(s) 795
EcoRII CCWGG 3 cut(s) 168, 364, 619
EcoRV GATATC 1 cut(s) 386
EcoT14I CCWWGG 2 cut(s) 476, 942
EcoT22I ATGCAT 1 cut(s) 823
ErhI CCWWGG 2 cut(s) 476, 942
FaeI CATG 7 cut(s) 119, 154, 164, 480, 640, 946, 986
FaqI GGGAC 2 cut(s) 453, 808
FatI CATG 7 cut(s) 115, 150, 160, 476, 636, 942, 982
Fnu4HI GCNGC 6 cut(s) 86, 276, 536, 539, 900, 1017
FokI GGATG 4 cut(s) 259, 317, 619, 938
Fsp4HI GCNGC 6 cut(s) 86, 276, 536, 539, 900, 1017
FspAI RTGCGCAY 1 cut(s) 783
FspBI CTAG 2 cut(s) 735, 993
FspI TGCGCA 1 cut(s) 783
GlaI GCGC 1 cut(s) 783
GluI GCNGC 6 cut(s) 86, 276, 536, 539, 900, 1017
GsaI CCCAGC 1 cut(s) 475
GsuI CTGGAG 2 cut(s) 152, 738
HaeIII GGCC 4 cut(s) 155, 418, 568, 1056
HapII CCGG 3 cut(s) 415, 1045, 1057
HgaI GACGC 2 cut(s) 175, 311
HhaI GCGC 1 cut(s) 784
Hin1II CATG 7 cut(s) 119, 154, 164, 480, 640, 946, 986
Hin6I GCGC 1 cut(s) 782
HinP1I GCGC 1 cut(s) 782
HincII GTYRAC 2 cut(s) 225, 339
HindII GTYRAC 2 cut(s) 225, 339
HinfI GANTC 2 cut(s) 221, 255
HpaII CCGG 3 cut(s) 415, 1045, 1057
HphI GGTGA 1 cut(s) 181
Hpy166II GTNNAC 4 cut(s) 175, 225, 328, 339
Hpy8I GTNNAC 4 cut(s) 175, 225, 328, 339
Hpy99I CGWCG 1 cut(s) 31
HpyAV CCTTC 4 cut(s) 25, 525, 738, 1023
HpyCH4III ACNGT 2 cut(s) 50, 428
HpyF10VI GCNNNNNNNGC 3 cut(s) 120, 407, 878
HpyF3I CTNAG 2 cut(s) 891, 964
Hsp92II CATG 7 cut(s) 119, 154, 164, 480, 640, 946, 986
HspAI GCGC 1 cut(s) 782
KroI GCCGGC 1 cut(s) 1044
KroNI GCCGGC 1 cut(s) 1046
LmnI GCTCC 4 cut(s) 310, 724, 886, 892
Lsp1109I GCAGC 5 cut(s) 97, 262, 550, 911, 1028
LweI GCATC 5 cut(s) 237, 769, 793, 830, 1003
MaeI CTAG 2 cut(s) 735, 993
MaeIII GTNAC 2 cut(s) 187, 603
MboII GAAGA 7 cut(s) 22, 25, 28, 85, 228, 305, 966
MfeI CAATTG 2 cut(s) 849, 1008
MlsI TGGCCA 1 cut(s) 155
MluCI AATT 9 cut(s) 59, 420, 504, 748, 801, 805, 849, 867, 1008
MluNI TGGCCA 1 cut(s) 155
MlyI GAGTC 1 cut(s) 230
Mox20I TGGCCA 1 cut(s) 155
Mph1103I ATGCAT 1 cut(s) 823
MroNI GCCGGC 1 cut(s) 1044
MscI TGGCCA 1 cut(s) 155
MseI TTAA 4 cut(s) 108, 765, 800, 804
MslI CAYNNNNRTG 2 cut(s) 789, 816
Msp20I TGGCCA 1 cut(s) 155
MspA1I CMGCKG 2 cut(s) 143, 758
MspI CCGG 3 cut(s) 415, 1045, 1057
MspR9I CCNGG 3 cut(s) 170, 366, 621
MunI CAATTG 2 cut(s) 849, 1008
MvaI CCWGG 3 cut(s) 170, 366, 621
MwoI GCNNNNNNNGC 3 cut(s) 120, 407, 878
NaeI GCCGGC 1 cut(s) 1046
NcoI CCATGG 2 cut(s) 476, 942
NgoMIV GCCGGC 1 cut(s) 1044
NlaIII CATG 7 cut(s) 119, 154, 164, 480, 640, 946, 986
NlaIV GGNNCC 2 cut(s) 363, 796
NmuCI GTSAC 2 cut(s) 187, 603
NsbI TGCGCA 1 cut(s) 783
NsiI ATGCAT 1 cut(s) 823
PacI TTAATTAA 1 cut(s) 804
PdiI GCCGGC 1 cut(s) 1046
PfeI GAWTC 1 cut(s) 255
PflMI CCANNNNNTGG 2 cut(s) 836, 851
PkrI GCNGC 6 cut(s) 87, 277, 537, 540, 901, 1018
PleI GAGTC 1 cut(s) 229
PpsI GAGTC 1 cut(s) 229
PpuMI RGGWCCY 1 cut(s) 795
PshBI ATTAAT 1 cut(s) 804
Psp5II RGGWCCY 1 cut(s) 795
Psp6I CCWGG 3 cut(s) 168, 364, 619
PspFI CCCAGC 1 cut(s) 471
PspGI CCWGG 3 cut(s) 168, 364, 619
PspN4I GGNNCC 2 cut(s) 363, 796
PspPI GGNCC 5 cut(s) 172, 795, 827, 842, 854
PspPPI RGGWCCY 1 cut(s) 795
PstI CTGCAG 1 cut(s) 436
PvuII CAGCTG 1 cut(s) 758
RsaI GTAC 1 cut(s) 693
RsaNI GTAC 1 cut(s) 692
RseI CAYNNNNRTG 2 cut(s) 789, 816
SaqAI TTAA 4 cut(s) 108, 765, 800, 804
SatI GCNGC 6 cut(s) 86, 276, 536, 539, 900, 1017
Sau96I GGNCC 5 cut(s) 172, 795, 827, 842, 854
SchI GAGTC 1 cut(s) 230
ScrFI CCNGG 3 cut(s) 170, 366, 621
SfaNI GCATC 5 cut(s) 237, 769, 793, 830, 1003
SfcI CTRYAG 1 cut(s) 432
SinI GGWCC 5 cut(s) 172, 795, 827, 842, 854
SmiMI CAYNNNNRTG 2 cut(s) 789, 816
SpeI ACTAGT 1 cut(s) 734
Sse9I AATT 9 cut(s) 59, 420, 504, 748, 801, 805, 849, 867, 1008
SsiI CCGC 4 cut(s) 141, 536, 617, 860
SspMI CTAG 2 cut(s) 735, 993
StyD4I CCNGG 3 cut(s) 168, 364, 619
StyI CCWWGG 2 cut(s) 476, 942
TaaI ACNGT 2 cut(s) 50, 428
TaqI TCGA 2 cut(s) 29, 371
TasI AATT 9 cut(s) 59, 420, 504, 748, 801, 805, 849, 867, 1008
TauI GCSGC 1 cut(s) 538
TfiI GAWTC 1 cut(s) 255
Tru1I TTAA 4 cut(s) 108, 765, 800, 804
Tru9I TTAA 4 cut(s) 108, 765, 800, 804
TseFI GTSAC 2 cut(s) 187, 603
TseI GCWGC 5 cut(s) 85, 275, 538, 899, 1016
Tsp45I GTSAC 2 cut(s) 187, 603
TspDTI ATGAA 2 cut(s) 149, 1002
TspGWI ACGGA 1 cut(s) 242
Van91I CCANNNNNTGG 2 cut(s) 836, 851
VpaK11BI GGWCC 5 cut(s) 172, 795, 827, 842, 854
VspI ATTAAT 1 cut(s) 804
XapI RAATTY 2 cut(s) 59, 748
XcmI CCANNNNNNNNNTGG 1 cut(s) 350
XspI CTAG 2 cut(s) 735, 993
Zsp2I ATGCAT 1 cut(s) 823
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.