RLG00000032579

Protein SUPPRESSOR OF FRI

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0006315
Physical Location & Seq
Forward (+)
16708944 .. 16713053
4110 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000032579

Sequence Viewer

Length: 1095 bp
ATGGGAAAGAAGAAGAAGAGGGTGGCCTCGAAGGTGTGGTGTTACTACTGCGATAGGGAGTTTGACGACGAGAAGATATTGGTGCAGCACCAGAAAGCTAAGCACTTCAAGTGCCATGTCTGCCACAAGAAGCTTTCTACAGCTGGGGGTATGGCCATTCATGTTCTCCAGGTCCACAAAGAGAACGTCACCAAGGTTCCCAATGCAAAGGCTGGGAGGGAATCGACAGATATTGAAATATATGGGATGCAAGGAATCCCACCTGACGTCTTAGCTGCACATTATGGAGAGGAAGAAGACGACGCCCCGTCAAAAATGGCTAAAGTGGAAATCCCATCAACTCAGCTTGTTAGCGGTGTGGTGCCAGGTTCAATGGGGACATATCCTCCCCAACCACCTTTTGGAATGCGGCCAATATACAATTCATCGGCCCCAATGCCTCACAACGGTTGGCAAGTTGCACCTCGTCCCCAGCCTTGGTATCCACAGCCTTTGGCAGTCTCTGTTCCTCTGCCTTCCGGAATGGGTTATTCACAACAGCCATTGTTTCCTGTGCAGCCACCTCTGCCATCAACAACATCCCCCGGACTTCAATCTTCACATGTAACTCCTCCTGGACTGCCTGCATCTATGCATCCTGTACCTGTATCACAACCTCTGTTTCCTGTTGTTGGTGTCAATAATTTACCAACTCAAAGTTCTCCATTTTCTGCACCCATGCTTTCAACAAATAATCCATCAAACCCTCTAGGCTCGATGGATGCACATCAGGGCGCTAGAACTTCAATTCCAAATAGTTACCATGCTTCAAATATTCCAGGTGGGAACTTCAATAATTCACACTCTTATGCATCTGGTCCAAATACTGGTGGCCCATCAATTGGACCACCCCCTGTAATAGCAAACAAAGCTCCCCAGCCAGCCACTAACGAGGTCTATTTAGTTTGGGATGATGAGGCTATGTCCATGGAGGAAAGAAGAATGTCCTTAGCGAAGTATCAGGTGCATGATGAAACTACCCAGATGAGCTCAATCGATGCAGCCATAGACAGAAGGATTTTGGAGAGCAGGCTTGCTGGTCGAATGGCATTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000070 GO:0000075 GO:0000226 GO:0000278 GO:0000280 GO:0000775 GO:0000776 GO:0000819 GO:0001578 GO:0003674 GO:0003700 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005694 GO:0005730 GO:0005856 GO:0006355 GO:0006996 GO:0007010 GO:0007017 GO:0007049 GO:0007051 GO:0007052 GO:0007059 GO:0007088 GO:0007093 GO:0007094 GO:0007346 GO:0008017 GO:0008092 GO:0008150 GO:0008270 GO:0008608 GO:0009889 GO:0009987 GO:0010468 GO:0010556 GO:0010564 GO:0010639 GO:0010948 GO:0010965 GO:0015631 GO:0016043 GO:0019219 GO:0019222 GO:0022402 GO:0022607 GO:0030071 GO:0031109 GO:0031323 GO:0031326 GO:0031577 GO:0031647 GO:0031974 GO:0031981 GO:0032991 GO:0033043 GO:0033044 GO:0033045 GO:0033046 GO:0033047 GO:0033048 GO:0034622 GO:0043167 GO:0043169 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043933 GO:0044085 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044430 GO:0044446 GO:0044464 GO:0045786 GO:0045839 GO:0045841 GO:0045930 GO:0046785 GO:0046872 GO:0046914 GO:0048285 GO:0048519 GO:0048523 GO:0050789 GO:0050794 GO:0050821 GO:0051128 GO:0051129 GO:0051171 GO:0051225 GO:0051252 GO:0051258 GO:0051276 GO:0051726 GO:0051783 GO:0051784 GO:0051983 GO:0051985 GO:0060255 GO:0065003 GO:0065007 GO:0065008 GO:0070013 GO:0070925 GO:0071173 GO:0071174 GO:0071840 GO:0080090 GO:0090307 GO:0097435 GO:0098687 GO:0098813 GO:0140014 GO:0140110 GO:1901987 GO:1901988 GO:1901990 GO:1901991 GO:1902099 GO:1902100 GO:1902850 GO:1903047 GO:1903506 GO:1905818 GO:1905819 GO:1990047 GO:2000112 GO:2000816 GO:2001141 GO:2001251
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

365

Amino Acids

39.42

Weight (kDa)

8.25

Isoelectric Point (pI)

67.65

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 270
AccB1I GGYRCC 1 cut(s) 361
AccB7I CCANNNNNTGG 3 cut(s) 401, 866, 881
AccIII TCCGGA 1 cut(s) 518
AciI CCGC 2 cut(s) 354, 409
AcoI YGGCCR 2 cut(s) 153, 410
AcyI GRCGYC 2 cut(s) 267, 303
AfaI GTAC 1 cut(s) 642
AfiI CCNNNNNNNGG 6 cut(s) 401, 446, 477, 671, 866, 881
AflIII ACRYGT 1 cut(s) 601
AgsI TTSAA 8 cut(s) 109, 236, 372, 593, 726, 786, 810, 832
AhdI GACNNNNNGTC 1 cut(s) 307
AjnI CCWGG 4 cut(s) 168, 364, 613, 817
AjuI GAANNNNNNNTTGG 4 cut(s) 406, 438, 682, 714
AloI GAACNNNNNNTCC 2 cut(s) 772, 804
AluBI AGCT 7 cut(s) 98, 133, 143, 275, 346, 911, 1029
AluI AGCT 7 cut(s) 98, 133, 143, 275, 346, 911, 1029
Alw21I GWGCWC 1 cut(s) 1031
Alw26I GTCTC 1 cut(s) 505
AlwNI CAGNNNCTG 1 cut(s) 503
Aor13HI TCCGGA 1 cut(s) 518
AoxI GGCC 5 cut(s) 24, 153, 410, 429, 871
ApeKI GCWGC 4 cut(s) 85, 275, 556, 1040
ArsI GACNNNNNNTTYG 2 cut(s) 171, 203
AspLEI GCGC 1 cut(s) 776
AspS9I GGNCC 5 cut(s) 172, 430, 857, 872, 884
AsuC2I CCSGG 1 cut(s) 585
AsuHPI GGTGA 1 cut(s) 181
AvaII GGWCC 3 cut(s) 172, 857, 884
BalI TGGCCA 1 cut(s) 155
BanI GGYRCC 1 cut(s) 361
BanII GRGCYC 1 cut(s) 1031
BbsI GAAGAC 1 cut(s) 303
Bbv12I GWGCWC 1 cut(s) 1031
BbvI GCAGC 4 cut(s) 97, 262, 568, 1052
BccI CCATC 5 cut(s) 343, 577, 745, 751, 883
BciT130I CCWGG 4 cut(s) 170, 366, 615, 819
BciVI GTATCC 1 cut(s) 492
BcnI CCSGG 1 cut(s) 585
BcoDI GTCTC 1 cut(s) 505
BfaI CTAG 2 cut(s) 749, 777
BfmI CTRYAG 1 cut(s) 138
BfoI RGCGCY 1 cut(s) 777
BfuI GTATCC 1 cut(s) 492
BisI GCNGC 5 cut(s) 86, 276, 410, 557, 1041
BlpI GCTNAGC 1 cut(s) 99
BlsI GCNGC 5 cut(s) 87, 277, 411, 558, 1042
Bme1390I CCNGG 5 cut(s) 170, 366, 585, 615, 819
Bme18I GGWCC 3 cut(s) 172, 857, 884
BmeRI GACNNNNNGTC 1 cut(s) 307
BmgT120I GGNCC 5 cut(s) 172, 430, 857, 872, 884
BmiI GGNNCC 3 cut(s) 198, 363, 432
BmrFI CCNGG 5 cut(s) 170, 366, 585, 615, 819
BmsI GCATC 6 cut(s) 237, 635, 643, 751, 860, 1027
BpiI GAAGAC 1 cut(s) 303
BpmI CTGGAG 1 cut(s) 152
Bpu10I CCTNAGC 1 cut(s) 988
Bpu1102I GCTNAGC 1 cut(s) 99
BpuMI CCSGG 1 cut(s) 585
Bsa29I ATCGAT 1 cut(s) 1035
BsaBI GATNNNNATC 1 cut(s) 765
BsaHI GRCGYC 2 cut(s) 267, 303
BsaJI CCNNGG 4 cut(s) 192, 476, 583, 966
BsaWI WCCGGW 1 cut(s) 518
BsaXI ACNNNNNCTCC 2 cut(s) 370, 400
Bsc4I CCNNNNNNNGG 6 cut(s) 401, 446, 477, 671, 866, 881
Bse1I ACTGG 1 cut(s) 871
Bse8I GATNNNNATC 1 cut(s) 765
BseAI TCCGGA 1 cut(s) 518
BseBI CCWGG 4 cut(s) 170, 366, 615, 819
BseCI ATCGAT 1 cut(s) 1035
BseDI CCNNGG 4 cut(s) 192, 476, 583, 966
BseGI GGATG 5 cut(s) 252, 578, 634, 766, 955
BseJI GATNNNNATC 1 cut(s) 765
BseLI CCNNNNNNNGG 6 cut(s) 401, 446, 477, 671, 866, 881
BseMII CTCAG 1 cut(s) 356
BseNI ACTGG 1 cut(s) 871
BseRI GAGGAG 1 cut(s) 600
BseXI GCAGC 4 cut(s) 97, 262, 568, 1052
BseYI CCCAGC 4 cut(s) 143, 212, 471, 915
BsgI GTGCAG 4 cut(s) 104, 261, 575, 696
BshFI GGCC 5 cut(s) 26, 155, 412, 431, 873
BshNI GGYRCC 1 cut(s) 361
BshVI ATCGAT 1 cut(s) 1035
BsiHKAI GWGCWC 1 cut(s) 1031
BsiSI CCGG 2 cut(s) 519, 585
BslFI GGGAC 2 cut(s) 391, 453
BslI CCNNNNNNNGG 6 cut(s) 401, 446, 477, 671, 866, 881
BsmAI GTCTC 1 cut(s) 505
BsmFI GGGAC 2 cut(s) 391, 453
BsmI GAATGC 1 cut(s) 411
BsnI GGCC 5 cut(s) 26, 155, 412, 431, 873
Bsp1286I GDGCHC 1 cut(s) 1031
Bsp13I TCCGGA 1 cut(s) 518
Bsp1720I GCTNAGC 1 cut(s) 99
Bsp19I CCATGG 1 cut(s) 966
BspACI CCGC 2 cut(s) 354, 409
BspANI GGCC 5 cut(s) 26, 155, 412, 431, 873
BspCNI CTCAG 1 cut(s) 355
BspDI ATCGAT 1 cut(s) 1035
BspEI TCCGGA 1 cut(s) 518
BspLI GGNNCC 3 cut(s) 198, 363, 432
BspT107I GGYRCC 1 cut(s) 361
BsrI ACTGG 1 cut(s) 871
BssECI CCNNGG 4 cut(s) 192, 476, 583, 966
BssNI GRCGYC 2 cut(s) 267, 303
BssT1I CCWWGG 3 cut(s) 192, 476, 966
Bst2UI CCWGG 4 cut(s) 170, 366, 615, 819
Bst4CI ACNGT 1 cut(s) 449
Bst6I CTCTTC 1 cut(s) 11
BstACI GRCGYC 2 cut(s) 267, 303
BstC8I GCNNGC 4 cut(s) 624, 921, 1070, 1074
BstDEI CTNAG 4 cut(s) 99, 271, 342, 988
BstDSI CCRYGG 1 cut(s) 966
BstF5I GGATG 5 cut(s) 252, 578, 634, 766, 955
BstH2I RGCGCY 1 cut(s) 777
BstHHI GCGC 1 cut(s) 776
BstMAI GTCTC 1 cut(s) 505
BstMWI GCNNNNNNNGC 3 cut(s) 120, 565, 908
BstNI CCWGG 4 cut(s) 170, 366, 615, 819
BstNSI RCATGY 1 cut(s) 605
BstSCI CCNGG 5 cut(s) 168, 364, 583, 613, 817
BstSFI CTRYAG 1 cut(s) 138
BstV1I GCAGC 4 cut(s) 97, 262, 568, 1052
BstV2I GAAGAC 1 cut(s) 303
Bsu15I ATCGAT 1 cut(s) 1035
BsuI GTATCC 1 cut(s) 492
BsuRI GGCC 5 cut(s) 26, 155, 412, 431, 873
BsuTUI ATCGAT 1 cut(s) 1035
BtgI CCRYGG 1 cut(s) 966
BtsCI GGATG 5 cut(s) 252, 578, 634, 766, 955
Cac8I GCNNGC 4 cut(s) 624, 921, 1070, 1074
CaiI CAGNNNCTG 1 cut(s) 503
CfoI GCGC 1 cut(s) 776
Cfr13I GGNCC 5 cut(s) 172, 430, 857, 872, 884
ClaI ATCGAT 1 cut(s) 1035
CseI GACGC 1 cut(s) 311
Csp6I GTAC 1 cut(s) 641
CspCI CAANNNNNGTGG 2 cut(s) 474, 509
CviAII CATG 7 cut(s) 116, 161, 602, 718, 803, 967, 1007
CviQI GTAC 1 cut(s) 641
DdeI CTNAG 4 cut(s) 99, 271, 342, 988
DriI GACNNNNNGTC 1 cut(s) 307
EaeI YGGCCR 2 cut(s) 153, 410
Eam1104I CTCTTC 1 cut(s) 11
Eam1105I GACNNNNNGTC 1 cut(s) 307
EarI CTCTTC 1 cut(s) 11
Ecl136II GAGCTC 1 cut(s) 1029
Eco130I CCWWGG 3 cut(s) 192, 476, 966
Eco24I GRGCYC 1 cut(s) 1031
Eco47I GGWCC 3 cut(s) 172, 857, 884
Eco53kI GAGCTC 1 cut(s) 1029
EcoICRI GAGCTC 1 cut(s) 1029
EcoRII CCWGG 4 cut(s) 168, 364, 613, 817
EcoT14I CCWWGG 3 cut(s) 192, 476, 966
EcoT22I ATGCAT 2 cut(s) 636, 853
EcoT38I GRGCYC 1 cut(s) 1031
ErhI CCWWGG 3 cut(s) 192, 476, 966
FaeI CATG 7 cut(s) 119, 164, 605, 721, 806, 970, 1010
FaqI GGGAC 2 cut(s) 391, 453
FatI CATG 7 cut(s) 115, 160, 601, 717, 802, 966, 1006
Fnu4HI GCNGC 5 cut(s) 86, 276, 410, 557, 1041
FokI GGATG 5 cut(s) 259, 565, 621, 773, 962
FriOI GRGCYC 1 cut(s) 1031
Fsp4HI GCNGC 5 cut(s) 86, 276, 410, 557, 1041
FspBI CTAG 2 cut(s) 749, 777
GlaI GCGC 1 cut(s) 775
GluI GCNGC 5 cut(s) 86, 276, 410, 557, 1041
GsaI CCCAGC 4 cut(s) 147, 216, 475, 919
GsuI CTGGAG 1 cut(s) 152
HaeII RGCGCY 1 cut(s) 777
HaeIII GGCC 5 cut(s) 26, 155, 412, 431, 873
HapII CCGG 2 cut(s) 519, 585
HgaI GACGC 1 cut(s) 311
HhaI GCGC 1 cut(s) 776
Hin1I GRCGYC 2 cut(s) 267, 303
Hin1II CATG 7 cut(s) 119, 164, 605, 721, 806, 970, 1010
Hin6I GCGC 1 cut(s) 774
HinP1I GCGC 1 cut(s) 774
HindIII AAGCTT 1 cut(s) 131
HinfI GANTC 2 cut(s) 221, 255
HpaII CCGG 2 cut(s) 519, 585
HphI GGTGA 1 cut(s) 181
Hpy166II GTNNAC 1 cut(s) 175
Hpy188III TCNNGA 1 cut(s) 519
Hpy8I GTNNAC 1 cut(s) 175
Hpy99I CGWCG 2 cut(s) 71, 305
HpyAV CCTTC 3 cut(s) 25, 525, 1047
HpyCH4III ACNGT 1 cut(s) 449
HpyCH4IV ACGT 2 cut(s) 186, 267
HpyF10VI GCNNNNNNNGC 3 cut(s) 120, 565, 908
HpyF3I CTNAG 4 cut(s) 99, 271, 342, 988
HpySE526I ACGT 2 cut(s) 186, 267
Hsp92I GRCGYC 2 cut(s) 267, 303
Hsp92II CATG 7 cut(s) 119, 164, 605, 721, 806, 970, 1010
HspAI GCGC 1 cut(s) 774
Kpn2I TCCGGA 1 cut(s) 518
LmnI GCTCC 1 cut(s) 916
Lsp1109I GCAGC 4 cut(s) 97, 262, 568, 1052
LweI GCATC 6 cut(s) 237, 635, 643, 751, 860, 1027
MaeI CTAG 2 cut(s) 749, 777
MaeII ACGT 2 cut(s) 186, 267
MaeIII GTNAC 4 cut(s) 41, 187, 604, 797
MboII GAAGA 8 cut(s) 22, 25, 28, 85, 305, 308, 588, 990
MfeI CAATTG 1 cut(s) 879
MhlI GDGCHC 1 cut(s) 1031
MlsI TGGCCA 1 cut(s) 155
MluCI AATT 5 cut(s) 421, 682, 786, 835, 879
MluNI TGGCCA 1 cut(s) 155
Mox20I TGGCCA 1 cut(s) 155
Mph1103I ATGCAT 2 cut(s) 636, 853
MroI TCCGGA 1 cut(s) 518
MscI TGGCCA 1 cut(s) 155
MslI CAYNNNNRTG 1 cut(s) 846
Msp20I TGGCCA 1 cut(s) 155
MspA1I CMGCKG 1 cut(s) 143
MspI CCGG 2 cut(s) 519, 585
MspR9I CCNGG 5 cut(s) 170, 366, 585, 615, 819
MunI CAATTG 1 cut(s) 879
Mva1269I GAATGC 1 cut(s) 411
MvaI CCWGG 4 cut(s) 170, 366, 615, 819
MwoI GCNNNNNNNGC 3 cut(s) 120, 565, 908
NciI CCSGG 1 cut(s) 585
NcoI CCATGG 1 cut(s) 966
NlaIII CATG 7 cut(s) 119, 164, 605, 721, 806, 970, 1010
NlaIV GGNNCC 3 cut(s) 198, 363, 432
NmuCI GTSAC 1 cut(s) 187
NsiI ATGCAT 2 cut(s) 636, 853
NspI RCATGY 1 cut(s) 605
PciI ACATGT 1 cut(s) 601
PctI GAATGC 1 cut(s) 411
PfeI GAWTC 2 cut(s) 221, 255
PflMI CCANNNNNTGG 3 cut(s) 401, 866, 881
PfoI TCCNGGA 1 cut(s) 613
PkrI GCNGC 5 cut(s) 87, 277, 411, 558, 1042
PscI ACATGT 1 cut(s) 601
Psp124BI GAGCTC 1 cut(s) 1031
Psp6I CCWGG 4 cut(s) 168, 364, 613, 817
PspFI CCCAGC 4 cut(s) 143, 212, 471, 915
PspGI CCWGG 4 cut(s) 168, 364, 613, 817
PspN4I GGNNCC 3 cut(s) 198, 363, 432
PspPI GGNCC 5 cut(s) 172, 430, 857, 872, 884
PstNI CAGNNNCTG 1 cut(s) 503
PvuII CAGCTG 1 cut(s) 143
RsaI GTAC 1 cut(s) 642
RsaNI GTAC 1 cut(s) 641
RseI CAYNNNNRTG 1 cut(s) 846
SacI GAGCTC 1 cut(s) 1031
SatI GCNGC 5 cut(s) 86, 276, 410, 557, 1041
Sau96I GGNCC 5 cut(s) 172, 430, 857, 872, 884
ScrFI CCNGG 5 cut(s) 170, 366, 585, 615, 819
SduI GDGCHC 1 cut(s) 1031
SfaNI GCATC 6 cut(s) 237, 635, 643, 751, 860, 1027
SfcI CTRYAG 1 cut(s) 138
SinI GGWCC 3 cut(s) 172, 857, 884
SmiMI CAYNNNNRTG 1 cut(s) 846
Sse9I AATT 5 cut(s) 421, 682, 786, 835, 879
SsiI CCGC 2 cut(s) 354, 409
SspI AATATT 1 cut(s) 814
SspMI CTAG 2 cut(s) 749, 777
SstI GAGCTC 1 cut(s) 1031
StyD4I CCNGG 5 cut(s) 168, 364, 583, 613, 817
StyI CCWWGG 3 cut(s) 192, 476, 966
TaaI ACNGT 1 cut(s) 449
TaiI ACGT 2 cut(s) 189, 270
TaqI TCGA 5 cut(s) 29, 224, 755, 1035, 1081
TasI AATT 5 cut(s) 421, 682, 786, 835, 879
TauI GCSGC 1 cut(s) 412
TfiI GAWTC 2 cut(s) 221, 255
TseFI GTSAC 1 cut(s) 187
TseI GCWGC 4 cut(s) 85, 275, 556, 1040
Tsp45I GTSAC 1 cut(s) 187
TspDTI ATGAA 3 cut(s) 149, 414, 1026
Van91I CCANNNNNTGG 3 cut(s) 401, 866, 881
VpaK11BI GGWCC 3 cut(s) 172, 857, 884
XceI RCATGY 1 cut(s) 605
XcmI CCANNNNNNNNNTGG 1 cut(s) 398
XspI CTAG 2 cut(s) 749, 777
ZraI GACGTC 1 cut(s) 268
Zsp2I ATGCAT 2 cut(s) 636, 853
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.