MD05G1285400.v1.1

Belongs to the eukaryotic ribosomal protein eL20 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0008240
Physical Location & Seq
Forward (+)
41912877 .. 41914153
1277 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1285400.v1.1.491

Sequence Viewer

Length: 537 bp
ATGGGCGTTTTCAAGTTCCACCAGTACCAGGTCGTCGGGAGGAAGCTCCCCACCGAGACCGATGAGCAGCCGAAGATTTACAGAATGAAGCTGTGGGCCACCAACGAGGTTCGCGCCAAGTCCAAGTTCTGGTATTTTCTGAGGAAGCTGAAGAAGGTGAAGAAGAGCAATGGCCAAGTTCTTGCCATCAATGAGATCTTTGAGAAGAACCCGACAACCATCAAGAACTACGGTATCTGGTTGCGGTATCAAAGCAGGACGGGCTACCACAACATGTACAAGGAATACCGCGACACCACGCTAAATGGTGCGGTGGAGCAGATGTACATCGAGATGGCATCTCGCCACAGGGTCAGGTTTCCTTGTATCCAGATCATCAAGACCGCCACCATCCCCGCTAAGCTTTGCAAGAGGGAAAGCACCAAGCAATTCCACAACTCCAAGATCAAGTTCCCGTTGGTGTTCAGGAAGGTTAGACCACCATCCAGGAAGTTGAAGACTACGTACAAGGCGTCAAGGCCCAACTTGTTTATGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03010 map03010
Pfam Domains
Protein Families
Ribosomal_L18A

Protein Analysis

179

Amino Acids

21.36

Weight (kDa)

10.42

Isoelectric Point (pI)

29.96

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribosomal_L18A PF01775 7 - 128 4.2e-24 Ribosomal proteins 50S-L18Ae/60S-L20/60S-L18A
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 129
AccII CGCG 2 cut(s) 114, 291
AciI CCGC 5 cut(s) 244, 289, 311, 384, 396
AcoI YGGCCR 1 cut(s) 172
AcuI CTGAAG 1 cut(s) 170
AcyI GRCGYC 1 cut(s) 512
AfaI GTAC 4 cut(s) 26, 278, 326, 506
AfiI CCNNNNNNNGG 2 cut(s) 28, 129
AflIII ACRYGT 1 cut(s) 273
AgsI TTSAA 2 cut(s) 13, 496
AjnI CCWGG 2 cut(s) 27, 485
AjuI GAANNNNNNNTTGG 4 cut(s) 110, 142, 434, 466
AluBI AGCT 4 cut(s) 46, 91, 148, 403
AluI AGCT 4 cut(s) 46, 91, 148, 403
Alw26I GTCTC 1 cut(s) 50
AoxI GGCC 3 cut(s) 96, 172, 518
ApeKI GCWGC 1 cut(s) 67
AspLEI GCGC 1 cut(s) 116
AspS9I GGNCC 2 cut(s) 96, 519
AsuHPI GGTGA 1 cut(s) 169
BaeI ACNNNNGTAYC 2 cut(s) 217, 250
BalI TGGCCA 1 cut(s) 174
BarI GAAGNNNNNNTAC 2 cut(s) 488, 520
BbsI GAAGAC 1 cut(s) 503
BbvI GCAGC 1 cut(s) 79
BccI CCATC 5 cut(s) 194, 227, 328, 398, 490
BciT130I CCWGG 2 cut(s) 29, 487
BciVI GTATCC 1 cut(s) 377
BcoDI GTCTC 1 cut(s) 50
BfuI GTATCC 1 cut(s) 377
BglII AGATCT 1 cut(s) 195
BisI GCNGC 1 cut(s) 68
BlpI GCTNAGC 1 cut(s) 399
BlsI GCNGC 1 cut(s) 69
Bme1390I CCNGG 2 cut(s) 29, 487
BmgT120I GGNCC 2 cut(s) 96, 519
BmrFI CCNGG 2 cut(s) 29, 487
BmsI GCATC 1 cut(s) 347
BpiI GAAGAC 1 cut(s) 503
Bpu1102I GCTNAGC 1 cut(s) 399
BsaAI YACGTR 1 cut(s) 504
BsaBI GATNNNNATC 1 cut(s) 326
BsaHI GRCGYC 1 cut(s) 512
BsaI GGTCTC 1 cut(s) 50
BsaXI ACNNNNNCTCC 2 cut(s) 308, 338
Bsc4I CCNNNNNNNGG 2 cut(s) 28, 129
Bse1I ACTGG 1 cut(s) 22
Bse3DI GCAATG 1 cut(s) 175
Bse8I GATNNNNATC 1 cut(s) 326
BseBI CCWGG 2 cut(s) 29, 487
BseGI GGATG 2 cut(s) 390, 482
BseJI GATNNNNATC 1 cut(s) 326
BseLI CCNNNNNNNGG 2 cut(s) 28, 129
BseMI GCAATG 1 cut(s) 175
BseMII CTCAG 1 cut(s) 131
BseNI ACTGG 1 cut(s) 22
BseXI GCAGC 1 cut(s) 79
Bsh1236I CGCG 2 cut(s) 114, 291
BshFI GGCC 3 cut(s) 98, 174, 520
BslI CCNNNNNNNGG 2 cut(s) 28, 129
BsmAI GTCTC 1 cut(s) 50
BsnI GGCC 3 cut(s) 98, 174, 520
Bso31I GGTCTC 1 cut(s) 50
Bsp1407I TGTACA 2 cut(s) 276, 324
Bsp143I GATC 3 cut(s) 195, 372, 444
Bsp1720I GCTNAGC 1 cut(s) 399
BspACI CCGC 5 cut(s) 244, 289, 311, 384, 396
BspANI GGCC 3 cut(s) 98, 174, 520
BspCNI CTCAG 1 cut(s) 132
BspFNI CGCG 2 cut(s) 114, 291
BspQI GCTCTTC 1 cut(s) 158
BspTNI GGTCTC 1 cut(s) 50
BsrDI GCAATG 1 cut(s) 175
BsrGI TGTACA 2 cut(s) 276, 324
BsrI ACTGG 1 cut(s) 22
BssMI GATC 3 cut(s) 195, 372, 444
BssNI GRCGYC 1 cut(s) 512
Bst2UI CCWGG 2 cut(s) 29, 487
Bst4CI ACNGT 1 cut(s) 233
Bst6I CTCTTC 1 cut(s) 158
BstACI GRCGYC 1 cut(s) 512
BstAUI TGTACA 2 cut(s) 276, 324
BstBAI YACGTR 1 cut(s) 504
BstDEI CTNAG 2 cut(s) 140, 399
BstF5I GGATG 2 cut(s) 390, 482
BstFNI CGCG 2 cut(s) 114, 291
BstHHI GCGC 1 cut(s) 116
BstKTI GATC 3 cut(s) 198, 375, 447
BstMAI GTCTC 1 cut(s) 50
BstMBI GATC 3 cut(s) 195, 372, 444
BstMWI GCNNNNNNNGC 1 cut(s) 261
BstNI CCWGG 2 cut(s) 29, 487
BstNSI RCATGY 1 cut(s) 277
BstSCI CCNGG 2 cut(s) 27, 485
BstSNI TACGTA 1 cut(s) 504
BstUI CGCG 2 cut(s) 114, 291
BstV1I GCAGC 1 cut(s) 79
BstV2I GAAGAC 1 cut(s) 503
BstX2I RGATCY 1 cut(s) 195
BstYI RGATCY 1 cut(s) 195
BsuI GTATCC 1 cut(s) 377
BsuRI GGCC 3 cut(s) 98, 174, 520
BtsCI GGATG 2 cut(s) 390, 482
CfoI GCGC 1 cut(s) 116
Cfr13I GGNCC 2 cut(s) 96, 519
CseI GACGC 1 cut(s) 501
CsiI ACCWGGT 1 cut(s) 27
Csp6I GTAC 4 cut(s) 25, 277, 325, 505
CviAII CATG 1 cut(s) 274
CviJI RGCY 9 cut(s) 46, 70, 91, 98, 148, 174, 264, 403, 520
CviKI_1 RGCY 9 cut(s) 46, 70, 91, 98, 148, 174, 264, 403, 520
CviQI GTAC 4 cut(s) 25, 277, 325, 505
DdeI CTNAG 2 cut(s) 140, 399
DpnI GATC 3 cut(s) 197, 374, 446
DpnII GATC 3 cut(s) 195, 372, 444
EaeI YGGCCR 1 cut(s) 172
Eam1104I CTCTTC 1 cut(s) 158
EarI CTCTTC 1 cut(s) 158
Eco105I TACGTA 1 cut(s) 504
Eco31I GGTCTC 1 cut(s) 50
Eco57I CTGAAG 1 cut(s) 170
EcoRII CCWGG 2 cut(s) 27, 485
FaeI CATG 1 cut(s) 277
FaiI YATR 2 cut(s) 275, 533
FatI CATG 1 cut(s) 273
FauI CCCGC 1 cut(s) 403
Fnu4HI GCNGC 1 cut(s) 68
FokI GGATG 2 cut(s) 377, 469
Fsp4HI GCNGC 1 cut(s) 68
GlaI GCGC 1 cut(s) 115
GluI GCNGC 1 cut(s) 68
HaeIII GGCC 3 cut(s) 98, 174, 520
HgaI GACGC 1 cut(s) 501
HhaI GCGC 1 cut(s) 116
Hin1I GRCGYC 1 cut(s) 512
Hin1II CATG 1 cut(s) 277
Hin6I GCGC 1 cut(s) 114
HinP1I GCGC 1 cut(s) 114
HindIII AAGCTT 1 cut(s) 401
HphI GGTGA 1 cut(s) 169
Hpy188I TCNGA 1 cut(s) 141
Hpy188III TCNNGA 6 cut(s) 37, 223, 331, 370, 379, 466
Hpy99I CGWCG 1 cut(s) 38
HpyAV CCTTC 2 cut(s) 148, 463
HpyCH4III ACNGT 1 cut(s) 233
HpyCH4IV ACGT 1 cut(s) 503
HpyCH4V TGCA 1 cut(s) 408
HpyF10VI GCNNNNNNNGC 1 cut(s) 261
HpyF3I CTNAG 2 cut(s) 140, 399
HpySE526I ACGT 1 cut(s) 503
Hsp92I GRCGYC 1 cut(s) 512
Hsp92II CATG 1 cut(s) 277
HspAI GCGC 1 cut(s) 114
Kzo9I GATC 3 cut(s) 195, 372, 444
LguI GCTCTTC 1 cut(s) 158
LmnI GCTCC 2 cut(s) 51, 316
Lsp1109I GCAGC 1 cut(s) 79
LweI GCATC 1 cut(s) 347
MabI ACCWGGT 1 cut(s) 27
MaeII ACGT 1 cut(s) 503
MalI GATC 3 cut(s) 197, 374, 446
MboI GATC 3 cut(s) 195, 372, 444
MboII GAAGA 6 cut(s) 85, 163, 172, 175, 217, 508
MflI RGATCY 1 cut(s) 195
MlsI TGGCCA 1 cut(s) 174
MluCI AATT 1 cut(s) 428
MluNI TGGCCA 1 cut(s) 174
MnlI CCTC 4 cut(s) 33, 100, 135, 405
Mox20I TGGCCA 1 cut(s) 174
MscI TGGCCA 1 cut(s) 174
MslI CAYNNNNRTG 1 cut(s) 332
Msp20I TGGCCA 1 cut(s) 174
MspR9I CCNGG 2 cut(s) 29, 487
MvaI CCWGG 2 cut(s) 29, 487
MvnI CGCG 2 cut(s) 114, 291
MwoI GCNNNNNNNGC 1 cut(s) 261
NdeII GATC 3 cut(s) 195, 372, 444
NlaIII CATG 1 cut(s) 277
NspI RCATGY 1 cut(s) 277
PciI ACATGT 1 cut(s) 273
PciSI GCTCTTC 1 cut(s) 158
PcsI WCGNNNNNNNCGW 1 cut(s) 509
PflMI CCANNNNNTGG 1 cut(s) 129
PfoI TCCNGGA 1 cut(s) 485
PkrI GCNGC 1 cut(s) 69
Ppu21I YACGTR 1 cut(s) 504
PscI ACATGT 1 cut(s) 273
Psp6I CCWGG 2 cut(s) 27, 485
PspGI CCWGG 2 cut(s) 27, 485
PspPI GGNCC 2 cut(s) 96, 519
PsuI RGATCY 1 cut(s) 195
RsaI GTAC 4 cut(s) 26, 278, 326, 506
RsaNI GTAC 4 cut(s) 25, 277, 325, 505
RseI CAYNNNNRTG 1 cut(s) 332
SapI GCTCTTC 1 cut(s) 158
SatI GCNGC 1 cut(s) 68
Sau3AI GATC 3 cut(s) 195, 372, 444
Sau96I GGNCC 2 cut(s) 96, 519
ScrFI CCNGG 2 cut(s) 29, 487
SexAI ACCWGGT 1 cut(s) 27
SfaNI GCATC 1 cut(s) 347
SmiMI CAYNNNNRTG 1 cut(s) 332
SnaBI TACGTA 1 cut(s) 504
Sse9I AATT 1 cut(s) 428
SsiI CCGC 5 cut(s) 244, 289, 311, 384, 396
StyD4I CCNGG 2 cut(s) 27, 485
TaaI ACNGT 1 cut(s) 233
TaiI ACGT 1 cut(s) 506
TaqI TCGA 1 cut(s) 330
TaqII GACCGA 1 cut(s) 74
TasI AATT 1 cut(s) 428
TatI WGTACW 2 cut(s) 276, 324
TseI GCWGC 1 cut(s) 67
TspDTI ATGAA 1 cut(s) 101
Van91I CCANNNNNTGG 1 cut(s) 129
XceI RCATGY 1 cut(s) 277
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.