pycom10g21970

Belongs to the eukaryotic ribosomal protein eL20 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr10
OG0008240
Physical Location & Seq
Forward (+)
24512423 .. 24513904
1482 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom10g21970.2

Sequence Viewer

Length: 534 bp
ATGGGTGTTTTCAAGTTCCACCAGTACCAGGTCGTCGGGAGGAAGCTCCCCACCGAGACCGATGAGCAGCCGAAGATTTACAGAATGAAGCTGTGGGCCACCAATGAGGTTCGGGCCAAGTCCAAGTTCTGGTATTTTCTGAGGAAGCTGAAGAAGGTGAAGAAAAGCAATGGCCAAGTTCTTGCCATCAATGAGATCTTTGAGAAGAACCCAACAACCATCAAGAACTACGGTATCTGGTTGAGGTATCAAAGCCGGACAGGTTACCACAACATGTACAAGGAGTACCGTGACACCACTCTAAATGGTGCCGTGGAGCAGATGTACATTGAGATGGCATCTCGCCACAGGGTCAGGTTTCCTTGTATCCAGATCATCAAGACTGCCACCATCCCCGCTAAGCTTTGCAAGAGGGAAAGCACCAAGCAATTCCACAACTCGAAGATCAAGTTCCCGTTGGTGTTCAGGAAGGTTAGACCACCATCCAGGAAGTTGAAGACTACGTACAAGGCATCGAGGCCCAACTTGGGTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03010 map03010
Pfam Domains
Protein Families
Ribosomal_L18A

Protein Analysis

178

Amino Acids

21.14

Weight (kDa)

10.42

Isoelectric Point (pI)

30.07

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 308
AccB7I CCANNNNNTGG 1 cut(s) 129
AciI CCGC 1 cut(s) 396
AcoI YGGCCR 1 cut(s) 172
AcuI CTGAAG 1 cut(s) 170
AfaI GTAC 5 cut(s) 26, 278, 287, 326, 506
AfiI CCNNNNNNNGG 3 cut(s) 28, 129, 527
AflIII ACRYGT 1 cut(s) 273
AgsI TTSAA 2 cut(s) 13, 496
AjnI CCWGG 2 cut(s) 27, 485
AjuI GAANNNNNNNTTGG 2 cut(s) 110, 142
AluBI AGCT 4 cut(s) 46, 91, 148, 403
AluI AGCT 4 cut(s) 46, 91, 148, 403
Alw26I GTCTC 1 cut(s) 50
AoxI GGCC 4 cut(s) 96, 114, 172, 518
ApeKI GCWGC 1 cut(s) 67
AspS9I GGNCC 3 cut(s) 96, 114, 519
AsuHPI GGTGA 1 cut(s) 169
BaeI ACNNNNGTAYC 2 cut(s) 217, 250
BalI TGGCCA 1 cut(s) 174
BanI GGYRCC 1 cut(s) 308
BarI GAAGNNNNNNTAC 2 cut(s) 488, 520
BbsI GAAGAC 1 cut(s) 503
BbvI GCAGC 1 cut(s) 79
BccI CCATC 5 cut(s) 194, 227, 328, 398, 490
BceAI ACGGC 1 cut(s) 296
BciT130I CCWGG 2 cut(s) 29, 487
BciVI GTATCC 1 cut(s) 377
BcoDI GTCTC 1 cut(s) 50
BfuI GTATCC 1 cut(s) 377
BglII AGATCT 1 cut(s) 195
BisI GCNGC 1 cut(s) 68
BlpI GCTNAGC 1 cut(s) 399
BlsI GCNGC 1 cut(s) 69
Bme1390I CCNGG 2 cut(s) 29, 487
BmgT120I GGNCC 3 cut(s) 96, 114, 519
BmiI GGNNCC 1 cut(s) 310
BmrFI CCNGG 2 cut(s) 29, 487
BmsI GCATC 2 cut(s) 347, 521
BpiI GAAGAC 1 cut(s) 503
Bpu1102I GCTNAGC 1 cut(s) 399
BsaAI YACGTR 1 cut(s) 504
BsaI GGTCTC 1 cut(s) 50
BsaJI CCNNGG 1 cut(s) 312
BsaXI ACNNNNNCTCC 2 cut(s) 308, 338
Bsc4I CCNNNNNNNGG 3 cut(s) 28, 129, 527
Bse1I ACTGG 1 cut(s) 22
Bse3DI GCAATG 1 cut(s) 175
BseBI CCWGG 2 cut(s) 29, 487
BseDI CCNNGG 1 cut(s) 312
BseGI GGATG 2 cut(s) 390, 482
BseLI CCNNNNNNNGG 3 cut(s) 28, 129, 527
BseMI GCAATG 1 cut(s) 175
BseMII CTCAG 1 cut(s) 131
BseNI ACTGG 1 cut(s) 22
BseXI GCAGC 1 cut(s) 79
BshFI GGCC 4 cut(s) 98, 116, 174, 520
BshNI GGYRCC 1 cut(s) 308
BsiSI CCGG 1 cut(s) 256
BslI CCNNNNNNNGG 3 cut(s) 28, 129, 527
BsmAI GTCTC 1 cut(s) 50
BsnI GGCC 4 cut(s) 98, 116, 174, 520
Bso31I GGTCTC 1 cut(s) 50
Bsp1407I TGTACA 2 cut(s) 276, 324
Bsp143I GATC 3 cut(s) 195, 372, 444
Bsp1720I GCTNAGC 1 cut(s) 399
BspACI CCGC 1 cut(s) 396
BspANI GGCC 4 cut(s) 98, 116, 174, 520
BspCNI CTCAG 1 cut(s) 132
BspLI GGNNCC 1 cut(s) 310
BspT107I GGYRCC 1 cut(s) 308
BspTNI GGTCTC 1 cut(s) 50
BsrDI GCAATG 1 cut(s) 175
BsrGI TGTACA 2 cut(s) 276, 324
BsrI ACTGG 1 cut(s) 22
BssECI CCNNGG 1 cut(s) 312
BssMI GATC 3 cut(s) 195, 372, 444
Bst2UI CCWGG 2 cut(s) 29, 487
Bst4CI ACNGT 2 cut(s) 233, 290
BstAUI TGTACA 2 cut(s) 276, 324
BstBAI YACGTR 1 cut(s) 504
BstDEI CTNAG 2 cut(s) 140, 399
BstDSI CCRYGG 1 cut(s) 312
BstEII GGTNACC 1 cut(s) 263
BstF5I GGATG 2 cut(s) 390, 482
BstKTI GATC 3 cut(s) 198, 375, 447
BstMAI GTCTC 1 cut(s) 50
BstMBI GATC 3 cut(s) 195, 372, 444
BstNI CCWGG 2 cut(s) 29, 487
BstNSI RCATGY 1 cut(s) 277
BstPI GGTNACC 1 cut(s) 263
BstSCI CCNGG 2 cut(s) 27, 485
BstSNI TACGTA 1 cut(s) 504
BstV1I GCAGC 1 cut(s) 79
BstV2I GAAGAC 1 cut(s) 503
BstX2I RGATCY 1 cut(s) 195
BstYI RGATCY 1 cut(s) 195
BsuI GTATCC 1 cut(s) 377
BsuRI GGCC 4 cut(s) 98, 116, 174, 520
BtgI CCRYGG 1 cut(s) 312
BtsCI GGATG 2 cut(s) 390, 482
Cfr13I GGNCC 3 cut(s) 96, 114, 519
CsiI ACCWGGT 1 cut(s) 27
Csp6I GTAC 5 cut(s) 25, 277, 286, 325, 505
CviAII CATG 1 cut(s) 274
CviQI GTAC 5 cut(s) 25, 277, 286, 325, 505
DdeI CTNAG 2 cut(s) 140, 399
DpnI GATC 3 cut(s) 197, 374, 446
DpnII GATC 3 cut(s) 195, 372, 444
EaeI YGGCCR 1 cut(s) 172
Eco105I TACGTA 1 cut(s) 504
Eco31I GGTCTC 1 cut(s) 50
Eco57I CTGAAG 1 cut(s) 170
Eco91I GGTNACC 1 cut(s) 263
EcoO65I GGTNACC 1 cut(s) 263
EcoRII CCWGG 2 cut(s) 27, 485
FaeI CATG 1 cut(s) 277
FaiI YATR 1 cut(s) 275
FatI CATG 1 cut(s) 273
FauI CCCGC 1 cut(s) 403
Fnu4HI GCNGC 1 cut(s) 68
FokI GGATG 2 cut(s) 377, 469
Fsp4HI GCNGC 1 cut(s) 68
GluI GCNGC 1 cut(s) 68
HaeIII GGCC 4 cut(s) 98, 116, 174, 520
HapII CCGG 1 cut(s) 256
Hin1II CATG 1 cut(s) 277
HindIII AAGCTT 1 cut(s) 401
HpaII CCGG 1 cut(s) 256
HphI GGTGA 1 cut(s) 169
Hpy188I TCNGA 1 cut(s) 141
Hpy188III TCNNGA 5 cut(s) 37, 223, 370, 379, 466
Hpy99I CGWCG 1 cut(s) 38
HpyAV CCTTC 2 cut(s) 148, 463
HpyCH4III ACNGT 2 cut(s) 233, 290
HpyCH4IV ACGT 1 cut(s) 503
HpyCH4V TGCA 1 cut(s) 408
HpyF3I CTNAG 2 cut(s) 140, 399
HpySE526I ACGT 1 cut(s) 503
Hsp92II CATG 1 cut(s) 277
Kzo9I GATC 3 cut(s) 195, 372, 444
LmnI GCTCC 2 cut(s) 51, 316
Lsp1109I GCAGC 1 cut(s) 79
LweI GCATC 2 cut(s) 347, 521
MabI ACCWGGT 1 cut(s) 27
MaeII ACGT 1 cut(s) 503
MaeIII GTNAC 2 cut(s) 263, 290
MalI GATC 3 cut(s) 197, 374, 446
MboI GATC 3 cut(s) 195, 372, 444
MboII GAAGA 6 cut(s) 85, 163, 172, 217, 454, 508
MflI RGATCY 1 cut(s) 195
MlsI TGGCCA 1 cut(s) 174
MluCI AATT 1 cut(s) 428
MluNI TGGCCA 1 cut(s) 174
MnlI CCTC 6 cut(s) 33, 100, 135, 237, 405, 510
Mox20I TGGCCA 1 cut(s) 174
MscI TGGCCA 1 cut(s) 174
MslI CAYNNNNRTG 1 cut(s) 332
Msp20I TGGCCA 1 cut(s) 174
MspI CCGG 1 cut(s) 256
MspR9I CCNGG 2 cut(s) 29, 487
MvaI CCWGG 2 cut(s) 29, 487
NdeII GATC 3 cut(s) 195, 372, 444
NlaIII CATG 1 cut(s) 277
NlaIV GGNNCC 1 cut(s) 310
NmuCI GTSAC 1 cut(s) 290
NspI RCATGY 1 cut(s) 277
PciI ACATGT 1 cut(s) 273
PflMI CCANNNNNTGG 1 cut(s) 129
PfoI TCCNGGA 1 cut(s) 485
PkrI GCNGC 1 cut(s) 69
Ppu21I YACGTR 1 cut(s) 504
PscI ACATGT 1 cut(s) 273
Psp6I CCWGG 2 cut(s) 27, 485
PspEI GGTNACC 1 cut(s) 263
PspGI CCWGG 2 cut(s) 27, 485
PspN4I GGNNCC 1 cut(s) 310
PspPI GGNCC 3 cut(s) 96, 114, 519
PsuI RGATCY 1 cut(s) 195
RsaI GTAC 5 cut(s) 26, 278, 287, 326, 506
RsaNI GTAC 5 cut(s) 25, 277, 286, 325, 505
RseI CAYNNNNRTG 1 cut(s) 332
SatI GCNGC 1 cut(s) 68
Sau3AI GATC 3 cut(s) 195, 372, 444
Sau96I GGNCC 3 cut(s) 96, 114, 519
ScrFI CCNGG 2 cut(s) 29, 487
SexAI ACCWGGT 1 cut(s) 27
SfaNI GCATC 2 cut(s) 347, 521
SmiMI CAYNNNNRTG 1 cut(s) 332
SnaBI TACGTA 1 cut(s) 504
Sse9I AATT 1 cut(s) 428
SsiI CCGC 1 cut(s) 396
StyD4I CCNGG 2 cut(s) 27, 485
TaaI ACNGT 2 cut(s) 233, 290
TaiI ACGT 1 cut(s) 506
TaqI TCGA 2 cut(s) 440, 515
TaqII GACCGA 1 cut(s) 74
TasI AATT 1 cut(s) 428
TatI WGTACW 2 cut(s) 276, 324
TseFI GTSAC 1 cut(s) 290
TseI GCWGC 1 cut(s) 67
Tsp45I GTSAC 1 cut(s) 290
TspDTI ATGAA 1 cut(s) 101
Van91I CCANNNNNTGG 1 cut(s) 129
XceI RCATGY 1 cut(s) 277
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.