Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr05

Orthogroup ID

OG0012644

Physical Location & Seq

Genomic Coordinates Forward (+)

43513957 .. 43522321

Gene Structure 8365 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD05G1302500.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 849 bp

ATGTGCGGTCGGAAAGACGGGGAAATACGGCGAACAGTTGGTTGGGTCCCTGTCCCTCTCTGCTTCGTCTCACTCTCACTGTCCCCCAATTCCCTCTCAGATCTCATTCCACCATGTCTACCCTCCGCTCCCAAAGCCCTAACCACCTCGCTACTCCTCCTCTCCCCCCCCATCCCCAGCCCTACCCCTCCCACGCCCTCATTGCTCGTCTTCTCAGGCGGGACCGCCTTCAACTGCGTCGTCGAGGAACTCAAGAGTTTCACCACTCGCGTCGCCCATGTCCTTCCTGTTTCCGACGACGGCGGAAGCACGGCGGAGATTTTCCGCGTTCTTGGCGGTCCCGCTGTCGGAGATATACGGTCGAGATGCTTGAGATTGGCTGATAAGAGCACTTCCGAAGCGGTGGCCGTCCATAGATTGCTTGGCCACCGGTTAGCGCTGGATTCGCGGCAGGCCAAATCCGAATGGTATGATATTGTGGCAAGAGAACATTCTCTATGGACGGGAGCATCGAAATTCGAAACCATTCAGGGAAACCATTCGAGCTTTTCTGGCTTATTTTCCGAATCAAATTCCGGCGATCAGATGAATCATTTTGCTTCAGTAATGGCAGGTGTCTTCCCTGCCGCAAACCCAGTTTTCCTGGATCAGTTACGTAATGTGGACTGCGCGGTTTATGCCATGGGATCCCTCATCACTTGTATTTGCCCATCACTGGTATTGACTGGCATTGGGGAGATTATATCTTCAAAGTCCTGCCCGAAGGTGCTTTTGTTAAATGGTACCCGTGATCGAGAGACAAGTGGATTTTCAACTTCTTGCTTTGTAACTGCTATTACAGAGTCTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

UPF0052

Protein Analysis

283

Amino Acids

30.0

Weight (kDa)

6.58

Isoelectric Point (pI)

43.58

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
CofD	PF01933	70 - 149	1.1e-12	2-phospho-L-lactate transferase CofD
CofD	PF01933	209 - 281	2.1e-08	2-phospho-L-lactate transferase CofD

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0012644)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_6g52500
malus_domestica	MD05G1302500.v1.1 MD16G1033100.v1.1
rosa_chinensis	RchiOBHm_Chr6g0259491 RchiOBHm_Chr6g0290121
rosa_laevigata	RLG00000014201 RLG00000015278
rosa_multiflora	Rmu_sc0005711.1_g000010
rosa_roxburghii	Rroxscaffold_7G00177350
rosa_rugosa	Rorug06G0209700 Rorug06G0209900
rosa_samantha	Rh1CG249200 Rh5BG077800 Rh6AG320300 Rh6BG327600 Rh6CG094100 Rh6CG333700
rosa_wichuraiana	Rw6G027640 Rw6G027670

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	602
Acc36I	ACCTGC	1 cut(s)	602
Acc65I	GGTACC	1 cut(s)	782
AccB1I	GGYRCC	1 cut(s)	782
AccBSI	CCGCTC	1 cut(s)	128
AccI	GTMKAC	1 cut(s)	118
AccII	CGCG	4 cut(s)	270, 327, 448, 671
AclWI	GGATC	3 cut(s)	654, 681, 694
AcoI	YGGCCR	2 cut(s)	405, 424
AcsI	RAATTY	2 cut(s)	515, 571
AcuI	CTGAAG	1 cut(s)	585
AfaI	GTAC	1 cut(s)	784
AfeI	AGCGCT	1 cut(s)	438
AfiI	CCNNNNNNNGG	2 cut(s)	347, 715
AgeI	ACCGGT	1 cut(s)	429
AgsI	TTSAA	3 cut(s)	232, 750, 813
AjnI	CCWGG	1 cut(s)	642
AjuI	GAANNNNNNNTTGG	2 cut(s)	25, 57
AluBI	AGCT	1 cut(s)	546
AluI	AGCT	1 cut(s)	546
Alw21I	GWGCWC	1 cut(s)	392
Alw26I	GTCTC	2 cut(s)	73, 791
AlwI	GGATC	3 cut(s)	654, 681, 694
Aor51HI	AGCGCT	1 cut(s)	438
AoxI	GGCC	3 cut(s)	405, 424, 453
ApoI	RAATTY	2 cut(s)	515, 571
AsiGI	ACCGGT	1 cut(s)	429
Asp700I	GAANNNNTTC	2 cut(s)	525, 538
Asp718I	GGTACC	1 cut(s)	782
AspLEI	GCGC	2 cut(s)	439, 671
AspS9I	GGNCC	3 cut(s)	46, 222, 338
AsuHPI	GGTGA	1 cut(s)	253
AsuII	TTCGAA	1 cut(s)	519
AvaII	GGWCC	3 cut(s)	46, 222, 338
BalI	TGGCCA	1 cut(s)	426
BamHI	GGATCC	1 cut(s)	686
BanI	GGYRCC	1 cut(s)	782
BbsI	GAAGAC	2 cut(s)	202, 610
Bbv12I	GWGCWC	1 cut(s)	392
BccI	CCATC	2 cut(s)	179, 718
BceAI	ACGGC	4 cut(s)	44, 316, 327, 392
BciT130I	CCWGG	1 cut(s)	644
BcoDI	GTCTC	2 cut(s)	73, 791
BfoI	RGCGCY	1 cut(s)	440
BfuAI	ACCTGC	1 cut(s)	602
BglII	AGATCT	1 cut(s)	100
BisI	GCNGC	2 cut(s)	449, 627
BlsI	GCNGC	2 cut(s)	450, 628
Bme1390I	CCNGG	1 cut(s)	644
Bme18I	GGWCC	3 cut(s)	46, 222, 338
BmgT120I	GGNCC	3 cut(s)	46, 222, 338
BmiI	GGNNCC	6 cut(s)	47, 48, 223, 340, 688, 784
BmrFI	CCNGG	1 cut(s)	644
BmrI	ACTGGG	1 cut(s)	629
BmsI	GCATC	2 cut(s)	356, 518
BmuI	ACTGGG	1 cut(s)	629
BpiI	GAAGAC	2 cut(s)	202, 610
Bpu14I	TTCGAA	1 cut(s)	519
BpuEI	CTTGAG	2 cut(s)	236, 391
BsaAI	YACGTR	1 cut(s)	656
BsaJI	CCNNGG	1 cut(s)	681
BsaWI	WCCGGW	1 cut(s)	429
Bsc4I	CCNNNNNNNGG	2 cut(s)	347, 715
Bse118I	RCCGGY	1 cut(s)	429
Bse1I	ACTGG	3 cut(s)	635, 720, 730
Bse3DI	GCAATG	1 cut(s)	200
BseBI	CCWGG	1 cut(s)	644
BseDI	CCNNGG	1 cut(s)	681
BseGI	GGATG	1 cut(s)	171
BseLI	CCNNNNNNNGG	2 cut(s)	347, 715
BseMI	GCAATG	1 cut(s)	200
BseMII	CTCAG	2 cut(s)	111, 228
BseNI	ACTGG	3 cut(s)	635, 720, 730
BseRI	GAGGAG	2 cut(s)	146, 149
BseYI	CCCAGC	1 cut(s)	176
Bsh1236I	CGCG	4 cut(s)	270, 327, 448, 671
Bsh1285I	CGRYCG	2 cut(s)	10, 362
BshFI	GGCC	3 cut(s)	407, 426, 455
BshNI	GGYRCC	1 cut(s)	782
BshTI	ACCGGT	1 cut(s)	429
BsiEI	CGRYCG	2 cut(s)	10, 362
BsiHKAI	GWGCWC	1 cut(s)	392
BsiSI	CCGG	2 cut(s)	430, 576
BslFI	GGGAC	5 cut(s)	32, 38, 67, 235, 324
BslI	CCNNNNNNNGG	2 cut(s)	347, 715
BsmAI	GTCTC	2 cut(s)	73, 791
BsmBI	CGTCTC	1 cut(s)	73
BsmFI	GGGAC	5 cut(s)	32, 38, 67, 235, 324
BsnI	GGCC	3 cut(s)	407, 426, 455
Bsp119I	TTCGAA	1 cut(s)	519
Bsp1286I	GDGCHC	1 cut(s)	392
Bsp143I	GATC	5 cut(s)	100, 580, 646, 686, 790
Bsp19I	CCATGG	1 cut(s)	681
BspANI	GGCC	3 cut(s)	407, 426, 455
BspCNI	CTCAG	2 cut(s)	110, 227
BspFNI	CGCG	4 cut(s)	270, 327, 448, 671
BspLI	GGNNCC	6 cut(s)	47, 48, 223, 340, 688, 784
BspMI	ACCTGC	1 cut(s)	602
BspPI	GGATC	3 cut(s)	654, 681, 694
BspT104I	TTCGAA	1 cut(s)	519
BspT107I	GGYRCC	1 cut(s)	782
BsrBI	CCGCTC	1 cut(s)	128
BsrDI	GCAATG	1 cut(s)	200
BsrFI	RCCGGY	1 cut(s)	429
BsrI	ACTGG	3 cut(s)	635, 720, 730
BssAI	RCCGGY	1 cut(s)	429
BssECI	CCNNGG	1 cut(s)	681
BssMI	GATC	5 cut(s)	100, 580, 646, 686, 790
BssT1I	CCWWGG	1 cut(s)	681
Bst2UI	CCWGG	1 cut(s)	644
Bst4CI	ACNGT	3 cut(s)	37, 81, 360
BstBAI	YACGTR	1 cut(s)	656
BstBI	TTCGAA	1 cut(s)	519
BstC8I	GCNNGC	1 cut(s)	453
BstDEI	CTNAG	2 cut(s)	97, 214
BstDSI	CCRYGG	1 cut(s)	681
BstF5I	GGATG	1 cut(s)	171
BstFNI	CGCG	4 cut(s)	270, 327, 448, 671
BstH2I	RGCGCY	1 cut(s)	440
BstHHI	GCGC	2 cut(s)	439, 671
BstKTI	GATC	5 cut(s)	103, 583, 649, 689, 793
BstMAI	GTCTC	2 cut(s)	73, 791
BstMBI	GATC	5 cut(s)	100, 580, 646, 686, 790
BstMCI	CGRYCG	2 cut(s)	10, 362
BstMWI	GCNNNNNNNGC	6 cut(s)	134, 202, 333, 445, 552, 677
BstNI	CCWGG	1 cut(s)	644
BstSCI	CCNGG	1 cut(s)	642
BstSNI	TACGTA	1 cut(s)	656
BstUI	CGCG	4 cut(s)	270, 327, 448, 671
BstV2I	GAAGAC	2 cut(s)	202, 610
BstX2I	RGATCY	2 cut(s)	100, 686
BstYI	RGATCY	2 cut(s)	100, 686
BsuRI	GGCC	3 cut(s)	407, 426, 455
BtgI	CCRYGG	1 cut(s)	681
BtsCI	GGATG	1 cut(s)	171
BtsIMutI	CAGTG	2 cut(s)	77, 713
BveI	ACCTGC	1 cut(s)	602
Cac8I	GCNNGC	1 cut(s)	453
CfoI	GCGC	2 cut(s)	439, 671
Cfr10I	RCCGGY	1 cut(s)	429
Cfr13I	GGNCC	3 cut(s)	46, 222, 338
CseI	GACGC	2 cut(s)	226, 259
Csp6I	GTAC	1 cut(s)	783
CspAI	ACCGGT	1 cut(s)	429
CviAII	CATG	3 cut(s)	114, 278, 682
CviJI	RGCY	8 cut(s)	137, 180, 380, 407, 426, 455, 546, 555
CviKI_1	RGCY	8 cut(s)	137, 180, 380, 407, 426, 455, 546, 555
CviQI	GTAC	1 cut(s)	783
DdeI	CTNAG	2 cut(s)	97, 214
DpnI	GATC	5 cut(s)	102, 582, 648, 688, 792
DpnII	GATC	5 cut(s)	100, 580, 646, 686, 790
EaeI	YGGCCR	2 cut(s)	405, 424
EciI	GGCGGA	2 cut(s)	318, 329
Eco105I	TACGTA	1 cut(s)	656
Eco130I	CCWWGG	1 cut(s)	681
Eco47I	GGWCC	3 cut(s)	46, 222, 338
Eco47III	AGCGCT	1 cut(s)	438
Eco57I	CTGAAG	1 cut(s)	585
EcoO109I	RGGNCCY	1 cut(s)	46
EcoRII	CCWGG	1 cut(s)	642
EcoT14I	CCWWGG	1 cut(s)	681
ErhI	CCWWGG	1 cut(s)	681
Esp3I	CGTCTC	1 cut(s)	73
FaeI	CATG	3 cut(s)	117, 281, 685
FaiI	YATR	9 cut(s)	115, 279, 356, 414, 471, 499, 678, 683, 743
FaqI	GGGAC	5 cut(s)	32, 38, 67, 235, 324
FatI	CATG	3 cut(s)	113, 277, 681
FauI	CCCGC	2 cut(s)	212, 349
FblI	GTMKAC	1 cut(s)	118
Fnu4HI	GCNGC	2 cut(s)	449, 627
FokI	GGATG	1 cut(s)	158
Fsp4HI	GCNGC	2 cut(s)	449, 627
GlaI	GCGC	2 cut(s)	438, 670
GluI	GCNGC	2 cut(s)	449, 627
GsaI	CCCAGC	1 cut(s)	180
HaeII	RGCGCY	1 cut(s)	440
HaeIII	GGCC	3 cut(s)	407, 426, 455
HapII	CCGG	2 cut(s)	430, 576
HgaI	GACGC	2 cut(s)	226, 259
HhaI	GCGC	2 cut(s)	439, 671
Hin1II	CATG	3 cut(s)	117, 281, 685
Hin6I	GCGC	2 cut(s)	437, 669
HinP1I	GCGC	2 cut(s)	437, 669
HinfI	GANTC	4 cut(s)	443, 566, 589, 842
HpaII	CCGG	2 cut(s)	430, 576
HphI	GGTGA	1 cut(s)	253
Hpy166II	GTNNAC	2 cut(s)	119, 664
Hpy188I	TCNGA	8 cut(s)	12, 100, 295, 350, 397, 463, 565, 585
Hpy188III	TCNNGA	4 cut(s)	253, 363, 794, 846
Hpy8I	GTNNAC	2 cut(s)	119, 664
Hpy99I	CGWCG	5 cut(s)	242, 245, 275, 299, 302
HpyAV	CCTTC	3 cut(s)	238, 293, 757
HpyCH4III	ACNGT	3 cut(s)	37, 81, 360
HpyCH4IV	ACGT	1 cut(s)	655
HpyF10VI	GCNNNNNNNGC	6 cut(s)	134, 202, 333, 445, 552, 677
HpyF3I	CTNAG	2 cut(s)	97, 214
HpySE526I	ACGT	1 cut(s)	655
Hsp92II	CATG	3 cut(s)	117, 281, 685
HspAI	GCGC	2 cut(s)	437, 669
KflI	GGGWCCC	1 cut(s)	46
KpnI	GGTACC	1 cut(s)	786
Kzo9I	GATC	5 cut(s)	100, 580, 646, 686, 790
LmnI	GCTCC	2 cut(s)	133, 506
LweI	GCATC	2 cut(s)	356, 518
MaeII	ACGT	1 cut(s)	655
MaeIII	GTNAC	2 cut(s)	651, 826
MalI	GATC	5 cut(s)	102, 582, 648, 688, 792
MbiI	CCGCTC	1 cut(s)	128
MboI	GATC	5 cut(s)	100, 580, 646, 686, 790
MboII	GAAGA	3 cut(s)	202, 610, 738
MflI	RGATCY	2 cut(s)	100, 686
MhlI	GDGCHC	1 cut(s)	392
MlsI	TGGCCA	1 cut(s)	426
MluCI	AATT	3 cut(s)	88, 515, 571
MluNI	TGGCCA	1 cut(s)	426
MmeI	TCCRAC	2 cut(s)	318, 328
Mox20I	TGGCCA	1 cut(s)	426
MroXI	GAANNNNTTC	2 cut(s)	525, 538
MscI	TGGCCA	1 cut(s)	426
MseI	TTAA	1 cut(s)	776
Msp20I	TGGCCA	1 cut(s)	426
MspA1I	CMGCKG	1 cut(s)	344
MspI	CCGG	2 cut(s)	430, 576
MspR9I	CCNGG	1 cut(s)	644
MvaI	CCWGG	1 cut(s)	644
MvnI	CGCG	4 cut(s)	270, 327, 448, 671
MwoI	GCNNNNNNNGC	6 cut(s)	134, 202, 333, 445, 552, 677
NcoI	CCATGG	1 cut(s)	681
NdeII	GATC	5 cut(s)	100, 580, 646, 686, 790
NlaIII	CATG	3 cut(s)	117, 281, 685
NlaIV	GGNNCC	6 cut(s)	47, 48, 223, 340, 688, 784
NspV	TTCGAA	1 cut(s)	519
PaqCI	CACCTGC	1 cut(s)	602
PcsI	WCGNNNNNNNCGW	1 cut(s)	509
PdmI	GAANNNNTTC	2 cut(s)	525, 538
PfeI	GAWTC	3 cut(s)	443, 566, 589
PfoI	TCCNGGA	1 cut(s)	642
PinAI	ACCGGT	1 cut(s)	429
PkrI	GCNGC	2 cut(s)	450, 628
Ppu21I	YACGTR	1 cut(s)	656
PpuMI	RGGWCCY	1 cut(s)	46
Psp5II	RGGWCCY	1 cut(s)	46
Psp6I	CCWGG	1 cut(s)	642
PspFI	CCCAGC	1 cut(s)	176
PspGI	CCWGG	1 cut(s)	642
PspN4I	GGNNCC	6 cut(s)	47, 48, 223, 340, 688, 784
PspPI	GGNCC	3 cut(s)	46, 222, 338
PspPPI	RGGWCCY	1 cut(s)	46
PsuI	RGATCY	2 cut(s)	100, 686
RsaI	GTAC	1 cut(s)	784
RsaNI	GTAC	1 cut(s)	783
SaqAI	TTAA	1 cut(s)	776
SatI	GCNGC	2 cut(s)	449, 627
Sau3AI	GATC	5 cut(s)	100, 580, 646, 686, 790
Sau96I	GGNCC	3 cut(s)	46, 222, 338
ScrFI	CCNGG	1 cut(s)	644
SduI	GDGCHC	1 cut(s)	392
SetI	ASST	5 cut(s)	149, 548, 616, 658, 768
SfaNI	GCATC	2 cut(s)	356, 518
SfuI	TTCGAA	1 cut(s)	519
SinI	GGWCC	3 cut(s)	46, 222, 338
SmlI	CTYRAG	2 cut(s)	251, 370
SmoI	CTYRAG	2 cut(s)	251, 370
SnaBI	TACGTA	1 cut(s)	656
Sse9I	AATT	3 cut(s)	88, 515, 571
StyD4I	CCNGG	1 cut(s)	642
StyI	CCWWGG	1 cut(s)	681
TaaI	ACNGT	3 cut(s)	37, 81, 360
TaiI	ACGT	1 cut(s)	658
TaqI	TCGA	6 cut(s)	243, 362, 512, 519, 542, 793
TasI	AATT	3 cut(s)	88, 515, 571
TauI	GCSGC	2 cut(s)	451, 629
TfiI	GAWTC	3 cut(s)	443, 566, 589
Tru1I	TTAA	1 cut(s)	776
Tru9I	TTAA	1 cut(s)	776
TscAI	CASTG	2 cut(s)	84, 720
TspDTI	ATGAA	1 cut(s)	602
TspRI	CASTG	2 cut(s)	84, 720
VpaK11BI	GGWCC	3 cut(s)	46, 222, 338
XapI	RAATTY	2 cut(s)	515, 571
XcmI	CCANNNNNNNNNTGG	1 cut(s)	419
XmiI	GTMKAC	1 cut(s)	118
XmnI	GAANNNNTTC	2 cut(s)	525, 538

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.