Rh5BG077800

Uncharacterised protein family UPF0052

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5B
OG0012644
Physical Location & Seq
Reverse (-)
6591754 .. 6594615
2862 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5BG077800.1

Sequence Viewer

Length: 1053 bp
ATGTCGACTCTCCGCTCCCAAAACCCTAACCCTTCCTGCTCCTCCATTCCCAATCCCACCCCCGCCGCCCAGCCTTCCCTCCTCGTCTTCTCCGGCGGCACCGCCTTCAACGGCGTCGTCGAGGACCTCAAGACTTTCACCACCCGCGTCGCCCACGTCCTTCCCGTTTCCGACGACGGAGGCAGCACCGCCGAGATCGTCCGCGTCCTCGGTGGTCCGGCCGTCGGAGATATACGGTCCAGGTGCTTGAGATTGGCCGATAAAAGCACCTCCGAGGCCGTCGCCGTCCGGCAATTGCTCGGTCATCGTTTACCGCTTGATGCACGCCAAGCCAAATCCGAATGGTATGATATTGTGGAAGGAGAACACAATTTATGGACAGGAGTATCAAAGCCCTATAGGGAAACCATTCGAGCTTTTCTGGTTTATTTTCAGAATCAGATTCTCCGTCGATCTGATGAATCGTTTTGCTTCAGTAATGGCAGCTCTTCAGCTCCTGCACTCTCTTCGAGGATAAAGAGGGTATTCTACATGTCAAGTGAGGGCCAAAACTTGCTTCATGAGGTCTTCCCTGCTCCAAACCCAGCTGTGATGGATCAGCTACGTAATGTGGATTGCATTGTTTATGCCATGGGTTCTCTCTTGACTTCCGTTTGCCCATCACTGGTATTAACTGGCATTGGGGAGATAATATCTTCAAGGTCCTGCCCCAAGGTACTTTTGTTAAACGGTACCCATGATCGAGAGACAAGTGGCTTCTCAGCTTCTTGCTTTGTAACTGCTATTACAGATGCACTAAATCGAACATATGGAGCCCCTCATAATTGTCTCAAAAACCTTCCGAGTCATTATATCAATACCCTATTAGTGCCCAAAGATGGTGAGATACCTGTTAATGTTCAATGCTTAGCTGACCAAGGAATTTTTGGAGTGATTGTTGTTGACTCTGTACATGATCCAAAAGTAGGTGTGGTATTTGACCCCAAGTCGTTAATACAAGCCCTTGATGATTTGATAGGCAGACACATGCCTACAAATGTTAAGGTGGTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
UPF0052

Protein Analysis

350

Amino Acids

37.87

Weight (kDa)

6.75

Isoelectric Point (pI)

48.64

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CofD PF01933 29 - 293 2.5e-27 2-phospho-L-lactate transferase CofD
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 731
AccB1I GGYRCC 2 cut(s) 98, 731
AccBSI CCGCTC 1 cut(s) 15
AccI GTMKAC 1 cut(s) 5
AccII CGCG 2 cut(s) 147, 204
AciI CCGC 9 cut(s) 13, 63, 66, 96, 102, 145, 189, 202, 314
AclWI GGATC 2 cut(s) 603, 950
AcoI YGGCCR 2 cut(s) 219, 255
AcsI RAATTY 1 cut(s) 921
AcuI CTGAAG 2 cut(s) 457, 474
AcyI GRCGYC 1 cut(s) 114
AfaI GTAC 3 cut(s) 717, 733, 951
AfiI CCNNNNNNNGG 4 cut(s) 224, 664, 878, 965
AflIII ACRYGT 1 cut(s) 531
AgsI TTSAA 3 cut(s) 109, 699, 902
AhdI GACNNNNNGTC 1 cut(s) 985
AjiI CACGTC 1 cut(s) 157
AjnI CCWGG 1 cut(s) 239
AjuI GAANNNNNNNTTGG 2 cut(s) 540, 572
AluBI AGCT 7 cut(s) 416, 486, 494, 587, 601, 764, 911
AluI AGCT 7 cut(s) 416, 486, 494, 587, 601, 764, 911
Alw26I GTCTC 2 cut(s) 740, 833
AlwI GGATC 2 cut(s) 603, 950
AlwNI CAGNNNCTG 1 cut(s) 497
AoxI GGCC 4 cut(s) 219, 255, 276, 544
ApeKI GCWGC 2 cut(s) 183, 483
ApoI RAATTY 1 cut(s) 921
Asp700I GAANNNNTTC 1 cut(s) 408
Asp718I GGTACC 1 cut(s) 731
AspS9I GGNCC 5 cut(s) 124, 215, 237, 544, 702
AsuHPI GGTGA 2 cut(s) 130, 893
AvaII GGWCC 4 cut(s) 124, 215, 237, 702
BaeGI GKGCMC 1 cut(s) 873
BaeI ACNNNNGTAYC 4 cut(s) 369, 402, 707, 740
BanI GGYRCC 2 cut(s) 98, 731
BanII GRGCYC 1 cut(s) 817
BbsI GAAGAC 2 cut(s) 79, 559
BbvI GCAGC 2 cut(s) 195, 495
BccI CCATC 3 cut(s) 586, 667, 872
BceAI ACGGC 4 cut(s) 127, 206, 263, 269
BcgI CGANNNNNNTGC 2 cut(s) 489, 523
BciT130I CCWGG 1 cut(s) 241
BcoDI GTCTC 2 cut(s) 740, 833
BfmI CTRYAG 1 cut(s) 397
BisI GCNGC 4 cut(s) 66, 97, 184, 484
BlpI GCTNAGC 1 cut(s) 907
BlsI GCNGC 4 cut(s) 67, 98, 185, 485
Bme1390I CCNGG 1 cut(s) 241
Bme18I GGWCC 4 cut(s) 124, 215, 237, 702
BmeRI GACNNNNNGTC 1 cut(s) 985
BmgBI CACGTC 1 cut(s) 157
BmgT120I GGNCC 5 cut(s) 124, 215, 237, 544, 702
BmiI GGNNCC 3 cut(s) 100, 733, 814
BmrFI CCNGG 1 cut(s) 241
BmsI GCATC 2 cut(s) 310, 781
BpiI GAAGAC 2 cut(s) 79, 559
Bpu1102I GCTNAGC 1 cut(s) 907
BpuEI CTTGAG 2 cut(s) 113, 268
BsaAI YACGTR 1 cut(s) 605
BsaHI GRCGYC 1 cut(s) 114
BsaJI CCNNGG 5 cut(s) 208, 273, 630, 711, 916
BsaXI ACNNNNNCTCC 2 cut(s) 921, 951
Bsc4I CCNNNNNNNGG 4 cut(s) 224, 664, 878, 965
Bse1I ACTGG 2 cut(s) 669, 679
BseBI CCWGG 1 cut(s) 241
BseDI CCNNGG 5 cut(s) 208, 273, 630, 711, 916
BseLI CCNNNNNNNGG 4 cut(s) 224, 664, 878, 965
BseMII CTCAG 1 cut(s) 774
BseNI ACTGG 2 cut(s) 669, 679
BseRI GAGGAG 2 cut(s) 31, 71
BseSI GKGCMC 1 cut(s) 873
BseX3I CGGCCG 1 cut(s) 219
BseXI GCAGC 2 cut(s) 195, 495
BseYI CCCAGC 2 cut(s) 69, 583
BsgI GTGCAG 1 cut(s) 483
Bsh1236I CGCG 2 cut(s) 147, 204
Bsh1285I CGRYCG 1 cut(s) 222
BshFI GGCC 4 cut(s) 221, 257, 278, 546
BshNI GGYRCC 2 cut(s) 98, 731
BsiEI CGRYCG 1 cut(s) 222
BsiSI CCGG 3 cut(s) 93, 218, 289
BslI CCNNNNNNNGG 4 cut(s) 224, 664, 878, 965
BsmAI GTCTC 2 cut(s) 740, 833
BsnI GGCC 4 cut(s) 221, 257, 278, 546
Bsp1286I GDGCHC 2 cut(s) 817, 873
Bsp1407I TGTACA 1 cut(s) 949
Bsp143I GATC 5 cut(s) 195, 452, 595, 739, 955
Bsp1720I GCTNAGC 1 cut(s) 907
Bsp19I CCATGG 1 cut(s) 630
BspACI CCGC 9 cut(s) 13, 63, 66, 96, 102, 145, 189, 202, 314
BspANI GGCC 4 cut(s) 221, 257, 278, 546
BspCNI CTCAG 1 cut(s) 773
BspFNI CGCG 2 cut(s) 147, 204
BspHI TCATGA 1 cut(s) 559
BspLI GGNNCC 3 cut(s) 100, 733, 814
BspPI GGATC 2 cut(s) 603, 950
BspQI GCTCTTC 1 cut(s) 493
BspT107I GGYRCC 2 cut(s) 98, 731
BsrBI CCGCTC 1 cut(s) 15
BsrGI TGTACA 1 cut(s) 949
BsrI ACTGG 2 cut(s) 669, 679
BssECI CCNNGG 5 cut(s) 208, 273, 630, 711, 916
BssMI GATC 5 cut(s) 195, 452, 595, 739, 955
BssNI GRCGYC 1 cut(s) 114
BssT1I CCWWGG 3 cut(s) 630, 711, 916
Bst2UI CCWGG 1 cut(s) 241
Bst4CI ACNGT 2 cut(s) 237, 731
Bst6I CTCTTC 2 cut(s) 493, 511
BstACI GRCGYC 1 cut(s) 114
BstAUI TGTACA 1 cut(s) 949
BstBAI YACGTR 1 cut(s) 605
BstC8I GCNNGC 1 cut(s) 325
BstDEI CTNAG 2 cut(s) 760, 907
BstDSI CCRYGG 1 cut(s) 630
BstFNI CGCG 2 cut(s) 147, 204
BstKTI GATC 5 cut(s) 198, 455, 598, 742, 958
BstMAI GTCTC 2 cut(s) 740, 833
BstMBI GATC 5 cut(s) 195, 452, 595, 739, 955
BstMCI CGRYCG 1 cut(s) 222
BstMWI GCNNNNNNNGC 1 cut(s) 329
BstNI CCWGG 1 cut(s) 241
BstNSI RCATGY 2 cut(s) 535, 1030
BstSCI CCNGG 1 cut(s) 239
BstSFI CTRYAG 1 cut(s) 397
BstSLI GKGCMC 1 cut(s) 873
BstSNI TACGTA 1 cut(s) 605
BstUI CGCG 2 cut(s) 147, 204
BstV1I GCAGC 2 cut(s) 195, 495
BstV2I GAAGAC 2 cut(s) 79, 559
BstZI CGGCCG 1 cut(s) 219
BsuRI GGCC 4 cut(s) 221, 257, 278, 546
BtgI CCRYGG 1 cut(s) 630
BtrI CACGTC 1 cut(s) 157
BtsIMutI CAGTG 1 cut(s) 662
Cac8I GCNNGC 1 cut(s) 325
CaiI CAGNNNCTG 1 cut(s) 497
CciI TCATGA 1 cut(s) 559
Cfr13I GGNCC 5 cut(s) 124, 215, 237, 544, 702
CseI GACGC 3 cut(s) 103, 136, 193
Csp6I GTAC 3 cut(s) 716, 732, 950
CviAII CATG 6 cut(s) 532, 560, 631, 737, 953, 1027
CviQI GTAC 3 cut(s) 716, 732, 950
DdeI CTNAG 2 cut(s) 760, 907
DpnI GATC 5 cut(s) 197, 454, 597, 741, 957
DpnII GATC 5 cut(s) 195, 452, 595, 739, 955
DriI GACNNNNNGTC 1 cut(s) 985
EaeI YGGCCR 2 cut(s) 219, 255
EagI CGGCCG 1 cut(s) 219
Eam1104I CTCTTC 2 cut(s) 493, 511
Eam1105I GACNNNNNGTC 1 cut(s) 985
EarI CTCTTC 2 cut(s) 493, 511
EclXI CGGCCG 1 cut(s) 219
Eco105I TACGTA 1 cut(s) 605
Eco130I CCWWGG 3 cut(s) 630, 711, 916
Eco24I GRGCYC 1 cut(s) 817
Eco47I GGWCC 4 cut(s) 124, 215, 237, 702
Eco52I CGGCCG 1 cut(s) 219
Eco57I CTGAAG 2 cut(s) 457, 474
EcoO109I RGGNCCY 2 cut(s) 124, 702
EcoRII CCWGG 1 cut(s) 239
EcoT14I CCWWGG 3 cut(s) 630, 711, 916
EcoT38I GRGCYC 1 cut(s) 817
ErhI CCWWGG 3 cut(s) 630, 711, 916
FaeI CATG 6 cut(s) 535, 563, 634, 740, 956, 1030
FatI CATG 6 cut(s) 531, 559, 630, 736, 952, 1026
FauI CCCGC 2 cut(s) 70, 152
FauNDI CATATG 1 cut(s) 808
FblI GTMKAC 1 cut(s) 5
Fnu4HI GCNGC 4 cut(s) 66, 97, 184, 484
FriOI GRGCYC 1 cut(s) 817
Fsp4HI GCNGC 4 cut(s) 66, 97, 184, 484
GluI GCNGC 4 cut(s) 66, 97, 184, 484
GsaI CCCAGC 2 cut(s) 73, 587
HaeIII GGCC 4 cut(s) 221, 257, 278, 546
HapII CCGG 3 cut(s) 93, 218, 289
HgaI GACGC 3 cut(s) 103, 136, 193
Hin1I GRCGYC 1 cut(s) 114
Hin1II CATG 6 cut(s) 535, 563, 634, 740, 956, 1030
HincII GTYRAC 2 cut(s) 6, 943
HindII GTYRAC 2 cut(s) 6, 943
HinfI GANTC 6 cut(s) 7, 436, 442, 461, 844, 944
HpaII CCGG 3 cut(s) 93, 218, 289
HphI GGTGA 2 cut(s) 130, 893
Hpy166II GTNNAC 3 cut(s) 6, 311, 943
Hpy188I TCNGA 8 cut(s) 172, 227, 274, 340, 435, 441, 457, 843
Hpy188III TCNNGA 4 cut(s) 130, 560, 643, 743
Hpy8I GTNNAC 3 cut(s) 6, 311, 943
Hpy99I CGWCG 8 cut(s) 119, 122, 152, 176, 179, 227, 284, 453
HpyAV CCTTC 6 cut(s) 42, 84, 115, 170, 353, 848
HpyCH4III ACNGT 2 cut(s) 237, 731
HpyCH4IV ACGT 2 cut(s) 156, 604
HpyCH4V TGCA 4 cut(s) 323, 500, 618, 794
HpyF10VI GCNNNNNNNGC 1 cut(s) 329
HpyF3I CTNAG 2 cut(s) 760, 907
HpySE526I ACGT 2 cut(s) 156, 604
Hsp92I GRCGYC 1 cut(s) 114
Hsp92II CATG 6 cut(s) 535, 563, 634, 740, 956, 1030
KpnI GGTACC 1 cut(s) 735
Kzo9I GATC 5 cut(s) 195, 452, 595, 739, 955
LguI GCTCTTC 1 cut(s) 493
LmnI GCTCC 5 cut(s) 20, 44, 499, 580, 812
Lsp1109I GCAGC 2 cut(s) 195, 495
LweI GCATC 2 cut(s) 310, 781
MaeII ACGT 2 cut(s) 156, 604
MaeIII GTNAC 1 cut(s) 775
MalI GATC 5 cut(s) 197, 454, 597, 741, 957
MbiI CCGCTC 1 cut(s) 15
MboI GATC 5 cut(s) 195, 452, 595, 739, 955
MboII GAAGA 5 cut(s) 79, 480, 498, 559, 687
MfeI CAATTG 1 cut(s) 293
MhlI GDGCHC 2 cut(s) 817, 873
MluCI AATT 4 cut(s) 293, 370, 823, 921
MlyI GAGTC 2 cut(s) 853, 938
MmeI TCCRAC 2 cut(s) 195, 205
MroXI GAANNNNTTC 1 cut(s) 408
MseI TTAA 5 cut(s) 671, 725, 894, 992, 1041
MspA1I CMGCKG 1 cut(s) 587
MspI CCGG 3 cut(s) 93, 218, 289
MspR9I CCNGG 1 cut(s) 241
MunI CAATTG 1 cut(s) 293
MvaI CCWGG 1 cut(s) 241
MvnI CGCG 2 cut(s) 147, 204
MwoI GCNNNNNNNGC 1 cut(s) 329
NcoI CCATGG 1 cut(s) 630
NdeI CATATG 1 cut(s) 808
NdeII GATC 5 cut(s) 195, 452, 595, 739, 955
NlaIII CATG 6 cut(s) 535, 563, 634, 740, 956, 1030
NlaIV GGNNCC 3 cut(s) 100, 733, 814
NmeAIII GCCGAG 1 cut(s) 217
NspI RCATGY 2 cut(s) 535, 1030
PagI TCATGA 1 cut(s) 559
PciI ACATGT 1 cut(s) 531
PciSI GCTCTTC 1 cut(s) 493
PcsI WCGNNNNNNNCGW 2 cut(s) 117, 162
PdmI GAANNNNTTC 1 cut(s) 408
PfeI GAWTC 3 cut(s) 436, 442, 461
PkrI GCNGC 4 cut(s) 67, 98, 185, 485
PleI GAGTC 2 cut(s) 852, 938
PpsI GAGTC 2 cut(s) 852, 938
Ppu21I YACGTR 1 cut(s) 605
PpuMI RGGWCCY 2 cut(s) 124, 702
PscI ACATGT 1 cut(s) 531
Psp5II RGGWCCY 2 cut(s) 124, 702
Psp6I CCWGG 1 cut(s) 239
PspFI CCCAGC 2 cut(s) 69, 583
PspGI CCWGG 1 cut(s) 239
PspN4I GGNNCC 3 cut(s) 100, 733, 814
PspPI GGNCC 5 cut(s) 124, 215, 237, 544, 702
PspPPI RGGWCCY 2 cut(s) 124, 702
PstNI CAGNNNCTG 1 cut(s) 497
PvuII CAGCTG 1 cut(s) 587
RsaI GTAC 3 cut(s) 717, 733, 951
RsaNI GTAC 3 cut(s) 716, 732, 950
SalI GTCGAC 1 cut(s) 4
SapI GCTCTTC 1 cut(s) 493
SaqAI TTAA 5 cut(s) 671, 725, 894, 992, 1041
SatI GCNGC 4 cut(s) 66, 97, 184, 484
Sau3AI GATC 5 cut(s) 195, 452, 595, 739, 955
Sau96I GGNCC 5 cut(s) 124, 215, 237, 544, 702
SchI GAGTC 2 cut(s) 853, 938
ScrFI CCNGG 1 cut(s) 241
SduI GDGCHC 2 cut(s) 817, 873
SfaNI GCATC 2 cut(s) 310, 781
SfcI CTRYAG 1 cut(s) 397
SinI GGWCC 4 cut(s) 124, 215, 237, 702
SmlI CTYRAG 2 cut(s) 128, 247
SmoI CTYRAG 2 cut(s) 128, 247
SnaBI TACGTA 1 cut(s) 605
Sse9I AATT 4 cut(s) 293, 370, 823, 921
SsiI CCGC 9 cut(s) 13, 63, 66, 96, 102, 145, 189, 202, 314
StyD4I CCNGG 1 cut(s) 239
StyI CCWWGG 3 cut(s) 630, 711, 916
TaaI ACNGT 2 cut(s) 237, 731
TaiI ACGT 2 cut(s) 159, 607
TaqI TCGA 7 cut(s) 5, 120, 412, 451, 509, 742, 802
TaqII GACCGA 1 cut(s) 290
TasI AATT 4 cut(s) 293, 370, 823, 921
TatI WGTACW 1 cut(s) 949
TauI GCSGC 2 cut(s) 68, 99
TfiI GAWTC 3 cut(s) 436, 442, 461
Tru1I TTAA 5 cut(s) 671, 725, 894, 992, 1041
Tru9I TTAA 5 cut(s) 671, 725, 894, 992, 1041
TscAI CASTG 1 cut(s) 669
TseI GCWGC 2 cut(s) 183, 483
TspDTI ATGAA 2 cut(s) 474, 548
TspGWI ACGGA 3 cut(s) 192, 437, 640
TspRI CASTG 1 cut(s) 669
VpaK11BI GGWCC 4 cut(s) 124, 215, 237, 702
XapI RAATTY 1 cut(s) 921
XceI RCATGY 2 cut(s) 535, 1030
XcmI CCANNNNNNNNNTGG 1 cut(s) 923
XmiI GTMKAC 1 cut(s) 5
XmnI GAANNNNTTC 1 cut(s) 408
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.